A fluorescent immunoassay microfluidic chip with controllable reaction time

Abstract

The utility model relates to the technical field of fluorescent immunoassay microfluidic chips, and discloses a fluorescent immunoassay microfluidic chip with controllable reaction time, including a chip body, wherein piston devices and gas outlet blocking devices are respectively arranged on both sides of the chip body, and the gas outlet blocking devices and the piston devices are connected through a chip reaction area, wherein a plurality of antibody labeling areas are arranged in the chip reaction area. The utility model utilizes a method combining an outlet rubber plug and a waterproof breathable membrane, and when the outlet rubber plug blocks the outlet hole, the fluid in the chip reaction area is stationary and does not generate lateral flow, so that the design is advantageously applied to projects requiring different reaction times, such as the antibody binding time of project A is 5min, and the antibody binding time of project B is 8min, in this case, the project with a long reaction time can be driven with priority to sample reagents, and the project with a short reaction time can be driven with priority to sample reagents, so as to realize immunoassay of super-multiple projects, and the use effect is excellent.

Description

Technical Field

[0001] The utility model relates to the technical field of fluorescent immune microfluidic chips, in particular to a fluorescent immune microfluidic chip with controllable reaction time. Background Art

[0002] Traditional immunochromatographic tests are in the form of NC nitrocellulose membranes or hydrophilic microfluidics. The driving principle is based on capillary action, and a special quality control line area needs to be designed to verify that the flow process of the fluid is complete. In addition, among the currently public solutions, there is no scheme that can realize dozens of superimposed multiple immunoassays like this one. According to the current public information, the multi-test immunochips all have a unified reaction time, and it is impossible to customize the reaction time and timing of different projects. Utility Model Content

[0003] The utility model aims to solve the problems existing in the prior art and proposes a fluorescent immune microfluidic chip with controllable reaction time.

[0004] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0005] A fluorescent immune microfluidic chip with controllable reaction time comprises a chip body, wherein piston devices and air outlet blocking devices are respectively arranged on both sides of the chip body, the air outlet blocking device and the piston device are connected through a chip reaction area, and a plurality of antibody labeling areas are arranged in the chip reaction area.

[0006] As a preferred embodiment of the utility model, the piston device comprises a tube column and a piston column, and the piston column is slidably arranged in the tube column.

[0007] As a preferred embodiment of the utility model, the air outlet blocking device includes an air outlet pipe and a rubber plug, and the rubber plug is arranged in the pipe opening of the air outlet pipe.

[0008] As a preferred embodiment of the utility model, a mounting seat is provided at the bottom of the tube column, the mounting seat is hollow, absorbent cotton is provided inside the mounting seat, the bottom of the tube column is connected with the inner cavity of the mounting seat, and the chip reaction area is connected with the inner cavity of the mounting seat.

[0009] As a preferred embodiment of the utility model, a waterproof and breathable membrane is arranged on the top of the mounting seat, and the waterproof and breathable membrane is located below the piston column.

[0010] As a preferred embodiment of the utility model, the chip reaction area includes a chromatographic flow channel, which is connected to the outlet blocking device through a microchannel. The depth of the chromatographic flow channel is between 0.1-1mm and the width is between 0.05-2mm. The depth and width of the microchannel are both 0.1mm.

[0011] As a preferred embodiment of the utility model, a sample cavity is provided at the bottom of the air outlet pipe, and the microchannel is communicated with the sample cavity.

[0012] Compared with the prior art, the beneficial effects of the utility model are:

[0013] The utility model utilizes a method of combining an outlet rubber plug and a piston. When the outlet rubber plug blocks the outlet hole, the fluid in the chip reaction area is stationary and no lateral flow occurs. This is advantageous for designing and applying to projects that require different reaction times. For example, the antibody binding time for project A is 5 minutes, and the antibody binding time for project B is 8 minutes. In this case, the solution can be used to first drive the sample reagent for the project with a long reaction time, and then drive the sample reagent for the project with a short reaction time, thereby realizing immunoassays for a large number of projects with excellent results. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a three-dimensional schematic diagram of a fluorescent immunomicrofluidic chip with controllable reaction time proposed by the utility model;

[0015] Figure 2 This is a top view of a fluorescent immunoassay microfluidic chip with controllable reaction time proposed by the utility model.

[0016] In the figure: 1. Chip body; 2. Chip reaction area; 3. Antibody labeling area; 4. Air outlet pipe; 5. Rubber plug; 6. Tube column; 7. Piston column; 8. Mounting seat; 9. Water-absorbent cotton; 10. Waterproof and breathable membrane; 11. Chromatographic flow channel; 12. Microchannel; 13. Sample chamber. DETAILED DESCRIPTION

[0017] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0018] Example 1

[0019] Reference Figure 1 and Figure 2A fluorescent immunoassay microfluidic chip with controllable reaction time comprises a chip body 1, wherein a piston device and an air outlet blocking device are respectively arranged on both sides of the chip body 1, and the air outlet blocking device and the piston device are connected through a chip reaction area 2, and a plurality of antibody labeling areas 3 are arranged in the chip reaction area 2.

[0020] This chip is based on the ELISA double antibody sandwich principle, and the antigen in the test sample has a colorable marker in the labeling area. For example, antibodies 1 to 2 are the detection line area. Because this patent solution is non-traditional capillary drive, it uses piston drive, and the fluid flow channel is controllable, so there is no need to design a quality control line area. In this embodiment, only limited detection lines and reaction areas are displayed, but this solution has strong scalability. When it is implemented, it can be designed according to this solution and superimposed, that is, to achieve immune detection of multiple projects;

[0021] The working principle of this chip is as follows:

[0022] By utilizing the method of combining the outlet rubber plug 5 and the piston, when the outlet rubber plug blocks the outlet hole, the fluid in the chip reaction area 2 is stationary and no lateral flow occurs. When the outlet rubber plug 5 blocks the outlet, the fluid cannot reach the corresponding waste liquid chamber. Only after the outlet rubber plug 5 is released, the fluid will be driven by the piston to slowly flow the sample along the flow channel of the reaction area, passing through the labeling area and the antibody area along the way to produce an antigen-antibody immune reaction. This is beneficial for designing and applying to projects that require different reaction times. For example, the antibody binding time for project A is 5 minutes, and the antibody binding time for project B is 8 minutes. In this case, this solution can be used to first drive the sample reagent for the project with a long reaction time, and then drive the sample reagent for the project with a short reaction time. This is particularly suitable for large-scale multiple projects of lying screening and instant detection, and the use effect is excellent.

[0023] In this embodiment, the piston device includes a tube column 6 and a piston column 7. The piston column 7 is slidably disposed in the tube column 6. By pushing the piston column 7 to slide in the tube column 6, the flow of liquid in the chip reaction area 2 is achieved.

[0024] In this embodiment, the gas outlet blocking device includes an gas outlet pipe 4 and a rubber plug 5. The rubber plug 5 is arranged in the pipe opening of the gas outlet pipe 4. The rubber plug 5 is taken out from the gas outlet pipe 4, and the flow of liquid in the chip reaction area 2 can be driven by pushing the piston column 7 to slide in the tube column 6.

[0025] In this embodiment, a mounting seat 8 is provided at the bottom of the column 6, and the mounting seat 8 is hollow. Water-absorbing cotton 9 is provided inside the mounting seat 8. The bottom of the column 6 is connected with the inner cavity of the mounting seat 8, and the chip reaction area 2 is connected with the inner cavity of the mounting seat 8. The water-absorbing cotton 9 can effectively prevent false positives caused by fluid reflux.

[0026] In this embodiment, a waterproof breathable membrane 10 is provided on the top of the mounting seat 8 , and the waterproof breathable membrane 10 is located below the piston column 7 . The waterproof breathable membrane 10 facilitates gas to enter the mounting seat 8 while preventing liquid from flowing back into the pipe column 6 .

[0027] In this embodiment, the chip reaction area 2 includes a chromatographic channel 11, which is connected to the gas outlet blocking device through a microchannel 12. The depth of the chromatographic channel 11 is between 1 mm and the width is between 2 mm. The depth and width of the microchannel 12 are both 0.1 mm.

[0028] In this embodiment, a sample cavity 13 is disposed at the bottom of the gas outlet pipe 4 , and the microchannel 12 is connected to the sample cavity 13 , so that the fluid can enter the sample cavity 13 after detection.

[0029] Example 2

[0030] Reference Figure 1 and Figure 2 A fluorescent immunoassay microfluidic chip with controllable reaction time comprises a chip body 1, wherein a piston device and an air outlet blocking device are respectively arranged on both sides of the chip body 1, and the air outlet blocking device and the piston device are connected through a chip reaction area 2, and a plurality of antibody labeling areas 3 are arranged in the chip reaction area 2.

[0031] This chip is based on the ELISA double antibody sandwich principle, and the antigen in the test sample has a colorable marker in the labeling area. For example, antibodies 1 to 2 are the detection line area. Because this patent solution is non-traditional capillary drive, it uses piston drive, and the fluid flow channel is controllable, so there is no need to design a quality control line area. In this embodiment, only limited detection lines and reaction areas are displayed, but this solution has strong scalability. When it is implemented, it can be designed according to this solution and superimposed, that is, to achieve immune detection of multiple projects;

[0032] The working principle of this chip is as follows:

[0033] By utilizing the method of combining the outlet rubber plug 5 and the piston, when the outlet rubber plug blocks the outlet hole, the fluid in the chip reaction area 2 is stationary and no lateral flow occurs. When the outlet rubber plug 5 blocks the outlet, the fluid cannot reach the corresponding waste liquid chamber. Only after the outlet rubber plug 5 is released, the fluid will be driven by the piston to slowly flow the sample along the flow channel of the reaction area, passing through the labeling area and the antibody area along the way to produce an antigen-antibody immune reaction. This is beneficial for designing and applying to projects that require different reaction times. For example, the antibody binding time for project A is 5 minutes, and the antibody binding time for project B is 8 minutes. In this case, this solution can be used to first drive the sample reagent for the project with a long reaction time, and then drive the sample reagent for the project with a short reaction time. This is particularly suitable for large-scale multiple projects of lying screening and instant detection, and the use effect is excellent.

[0034] In this embodiment, the piston device includes a tube column 6 and a piston column 7. The piston column 7 is slidably disposed in the tube column 6. By pushing the piston column 7 to slide in the tube column 6, the flow of liquid in the chip reaction area 2 is achieved.

[0035] In this embodiment, the gas outlet blocking device includes an gas outlet pipe 4 and a rubber plug 5. The rubber plug 5 is arranged in the pipe opening of the gas outlet pipe 4. The rubber plug 5 is taken out from the gas outlet pipe 4, and the flow of liquid in the chip reaction area 2 can be driven by pushing the piston column 7 to slide in the tube column 6.

[0036] In this embodiment, a mounting seat 8 is provided at the bottom of the column 6, and the mounting seat 8 is hollow. Water-absorbing cotton 9 is provided inside the mounting seat 8. The bottom of the column 6 is connected with the inner cavity of the mounting seat 8, and the chip reaction area 2 is connected with the inner cavity of the mounting seat 8. The water-absorbing cotton 9 can effectively prevent false positives caused by fluid reflux.

[0037] In this embodiment, a waterproof breathable membrane 10 is provided on the top of the mounting seat 8 , and the waterproof breathable membrane 10 is located below the piston column 7 . The waterproof breathable membrane 10 facilitates gas to enter the mounting seat 8 while preventing liquid from flowing back into the pipe column 6 .

[0038] In this embodiment, the chip reaction area 2 includes a chromatographic channel 11, which is connected to the gas outlet blocking device through a microchannel 12. The depth of the chromatographic channel 11 is between 0.1 mm and the width is between 0.05 mm. The depth and width of the microchannel 12 are both 0.1 mm.

[0039] In this embodiment, a sample cavity 13 is disposed at the bottom of the gas outlet pipe 4 , and the microchannel 12 is connected to the sample cavity 13 , so that the fluid can enter the sample cavity 13 after detection.

[0040] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A fluorescent immunoassay microfluidic chip with controllable reaction time, comprising a chip body (1), characterized in that: A piston device and an air outlet blocking device are respectively arranged on both sides of the chip body (1); the air outlet blocking device and the piston device are connected through a chip reaction area (2); and a plurality of antibody labeling areas (3) are arranged in the chip reaction area (2).

2. The fluorescent immunoassay microfluidic chip with controllable reaction time according to claim 1, characterized in that: The piston device comprises a tubular column (6) and a piston column (7), and the piston column (7) is slidably arranged in the tubular column (6).

3. The fluorescent immunoassay microfluidic chip with controllable reaction time according to claim 2, characterized in that: The air outlet blocking device comprises an air outlet pipe (4) and a rubber plug (5), wherein the rubber plug (5) is arranged in the pipe opening of the air outlet pipe (4).

4. The fluorescent immunoassay microfluidic chip with controllable reaction time according to claim 2, characterized in that: A mounting seat (8) is arranged at the bottom of the tube column (6), the mounting seat (8) is hollow, absorbent cotton (9) is arranged inside the mounting seat (8), the bottom of the tube column (6) is connected to the inner cavity of the mounting seat (8), and the chip reaction area (2) is connected to the inner cavity of the mounting seat (8).

5. The fluorescent immunoassay microfluidic chip with controllable reaction time according to claim 4, characterized in that: A waterproof and breathable membrane (10) is arranged on the top of the mounting seat (8), and the waterproof and breathable membrane (10) is located below the piston column (7).

6. The fluorescent immunoassay microfluidic chip with controllable reaction time according to claim 1, characterized in that: The chip reaction area (2) comprises a chromatographic flow channel (11), the chromatographic flow channel (11) is connected to the gas outlet blocking device through a microchannel (12), the chromatographic flow channel (11) has a depth of 0.1-1 mm and a width of 0.05-2 mm, and the microchannel (12) has a depth and a width of 0.1 mm.

7. The fluorescent immunoassay microfluidic chip with controllable reaction time according to claim 3, characterized in that: A sample cavity (13) is provided at the bottom of the air outlet pipe (4), and the microchannel (12) is in communication with the sample cavity (13).

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