A residual hydrogen peroxide sampling and detection device

Abstract

The utility model relates to the technical field of hydrogen peroxide detection devices, and discloses a residual hydrogen peroxide sampling and detection device, comprising a sampling module, a reaction module, a cleaning and degassing module, a detection module and a waste liquid collection module which are connected in sequence, wherein the sampling module comprises a wiping sampling part and a gas-liquid sampling part and an extraction part which are connected to the wiping sampling part, wherein the wiping sampling part comprises a wiping sample container; the gas-liquid sampling part comprises a collecting pump and a gas collecting pipe and a liquid collecting pipe on the collecting pump and a collector which is connected to the wiping sample container; the reaction module comprises a reaction container which is connected to the cleaning and degassing module and a color changing part which is connected to the reaction container; the device can quickly collect, color and test residual hydrogen peroxide in engineering equipment, can quickly obtain results on site, can avoid the problem of decomposition of collected materials caused by discontinuity between the collecting process and the detecting process, so that the test results are more real and accurate, can perform multi-channel detection, and can process multiple samples at the same time to further improve the detection efficiency.

Description

Technical Field

[0001] The utility model relates to the technical field of hydrogen peroxide detection devices, in particular to a residual hydrogen peroxide sampling and detection device. Background Art

[0002] The residual hydrogen peroxide in engineering equipment is divided into many forms. Not only is the hydrogen peroxide gas dispersed everywhere in space, but hydrogen peroxide is also adsorbed on materials, such as stainless steel countertops, filling pre-filled needles, rubber gloves, packaging materials, rubber stoppers, and silicone tubes. In some containers, hydrogen peroxide will also diffuse and is not easily discharged with ventilation. These residual hydrogen peroxides will denature the amino acids in protein drugs. For example, proteins such as methionine, cysteine, histidine and tryptophan are easily oxidized by hydrogen peroxide, resulting in the destruction of protein structure, aggregation or inactivation of proteins. Therefore, it is necessary to test the residual hydrogen peroxide content in engineering equipment.

[0003] At present, the detection of residual hydrogen peroxide is mainly carried out by spectrophotometry after the color reaction between hydrogen peroxide and a color-changing agent. For example, a detection kit for residual hydrogen peroxide and a detection method thereof disclosed in Publication No. CN108072650A, in which 4-aminoantipyrine and a phenol solution are derivatized under the catalysis of hydrogen peroxide to generate a stable colored compound, and then a colorimetric reaction is performed to detect the hydrogen peroxide content in water; another example is an effervescent tablet for rapid detection of residual hydrogen peroxide in food disclosed in Publication No. CN103472054, in which the color-changing agent is made into a tablet When used, it is added to the sample to be tested for color change reaction, and then detected by ultraviolet spectrophotometry; currently, the detection of hydrogen peroxide is mainly concentrated in the field of color-changing agents, and there are very few devices for detecting residual hydrogen peroxide in engineering equipment; currently, the residual hydrogen peroxide detection environment in engineering equipment is relatively complex, and it is impossible to quickly test hydrogen peroxide adsorbed on the surface, hydrogen peroxide dispersed in the air, and hydrogen peroxide in liquid form. The test process requires manual sampling and testing, and the detection process is long and inefficient, which significantly reduces the efficiency of subsequent use of engineering equipment and reduces work efficiency. Summary of the invention

[0004] In order to overcome the problems of complex detection environment, inability to perform rapid detection and low detection efficiency when detecting residual hydrogen peroxide in engineering equipment in the prior art, the present invention provides a residual hydrogen peroxide sampling and detection device, which can easily cope with the complex sampling environment in engineering equipment by integrating three sampling structures of solid, liquid and gas on the detection device. The sampling structure, color development structure and detection structure of the device are an integrated structure, the sampling and detection process is simple and continuous, and the residual hydrogen peroxide inside the engineering equipment can be quickly detected, the detection efficiency is significantly improved, and the sampling process, extraction process, color development process and spectrophotometric detection are continuous, avoiding the problem of decomposition in the hydrogen peroxide transport process, so that the detection result is true and accurate.

[0005] The specific technical scheme of the present invention is:

[0006] A residual hydrogen peroxide sampling and detection device comprises a sampling module, a reaction module, a cleaning and degassing module, a detection module and a waste liquid collection module which are connected in sequence, wherein the sampling module comprises a wiping sampling part and a gas-liquid sampling part and an extraction part which are connected to the wiping sampling part, wherein the wiping sampling part comprises a wiping sample container; the gas-liquid sampling part comprises a collection pump and a gas collecting pipe and a liquid collecting pipe on the collection pump and a collector which is connected to the wiping sample container; the reaction module comprises a reaction container which is connected to the cleaning and degassing module and a color changing part which is connected to the reaction container, and the detection module is a spectrophotometer.

[0007] The utility model provides a residual hydrogen peroxide sampling and detection device, the wiping sampling part and the gas-liquid sampling part of the device integrate the gas sampling structure, the liquid sampling structure and the solid sampling structure, and can easily cope with the complex detection environment of engineering equipment; the device integrates the sampling structure, the color change reaction structure and the detection structure into one, so that the hydrogen peroxide can be continuously carried out from sampling, separation, color development to detection, avoiding the error of sample transportation loss, and significantly improving the accuracy and authenticity of the detection result; the device can quickly measure the content of residual hydrogen peroxide in the engineering equipment, significantly speed up the detection efficiency, significantly reduce the time required for detection, and improve the work efficiency of the subsequent engineering equipment; in addition, the device is also provided with a waste liquid collection module, which can collect waste generated in the sampling, detection and reaction processes, avoid secondary pollution in the detection process, and ensure the use of subsequent engineering equipment.

[0008] Preferably, the wipe sample container is provided with a wipe sample container drain port; the wipe sample container drain port is used to discharge the waste liquid and cleaning solution after extraction.

[0009] Preferably, the wipe sample container is also provided with an ultrasonic oscillation device detachably connected to the wipe sample container; the ultrasonic oscillation device can accelerate the extraction and separation speed of hydrogen peroxide in the collected sample by the extraction liquid, and the detachable connection can facilitate the replacement of the wipe sample container.

[0010] Preferably, the gas collecting pipe is made of glass, and the liquid collecting pipe is made of glass.

[0011] Preferably, the reaction container is provided with a reaction container drain port; the reaction container drain port is used to discharge excess color developing solution and cleaning solution.

[0012] Preferably, the reaction container is further provided with a stirring member detachably connected to the reaction container, and the stirring member comprises a magnetic stirrer and a magnetic rotor located in the reaction container; the stirring member can accelerate the reaction speed and improve the reaction efficiency.

[0013] Preferably, the extraction component includes an extraction liquid container and a first diaphragm pump connecting the extraction liquid container and the wipe sample container; the extraction liquid is stored in the extraction liquid container, and the extraction liquid in the extraction liquid container is pumped out by the diaphragm pump and transferred to the wipe sample container to extract and separate the hydrogen peroxide in the collected sample. The diaphragm pump can separate impurities in the extraction liquid to ensure the purity of the extractant entering the wipe sample container.

[0014] Preferably, the color-changing component includes a color-changing agent container and a second diaphragm pump connecting the color-changing agent container and the reaction container; a color developer that reacts with hydrogen peroxide to develop color is stored in the color-changing container, and the color developer is pumped out by the second diaphragm pump and transferred to the reaction container to undergo a color development reaction with the extracted hydrogen peroxide. The diaphragm pump can separate impurities in the color developer, thereby ensuring the purity of the extractant entering the reaction container, ensuring the clarity of the liquid during subsequent spectrophotometric detection, and ensuring the accuracy of the detection results.

[0015] Preferably, the waste liquid collection module is a waste liquid collection container, which is connected to the sampling module, reaction module and detection module. The waste liquid collector is used to collect waste and cleaning liquid in the sampling module, reaction module and detection module.

[0016] Preferably, the cleaning and degassing module includes a degassing cleaning machine, which is connected to the sampling module, the reaction module and the detection module; the cleaning and degassing module is used to clean the residues after the completion of the sampling module, the reaction module and the detection module to ensure the accuracy of each detection, and can also perform vacuum degassing during use to discharge the air in the device.

[0017] Compared with the prior art, this application has the following technical effects:

[0018] 1. The utility model integrates three sampling structures of air sampling, liquid sampling and solid sampling into one device, which can easily cope with the complex residual hydrogen peroxide detection environment in engineering equipment;

[0019] 2. The utility model integrates the sampling module, the reaction module and the detection module into one, which can quickly collect, color and test the residual hydrogen peroxide in the engineering equipment, and can quickly obtain the results on site. At the same time, it can avoid the problem of inaccurate results caused by the discontinuous collection process and the detection process, and the decomposition of the collected materials can be avoided, so that the test results are more real and accurate;

[0020] 3. The utility model can perform multi-channel detection, and can perform spectrophotometric detection on one sample while performing extraction and color development reaction on another sample. It can process multiple samples at the same time, which can further improve the detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a structural schematic diagram of the residual hydrogen peroxide sampling and detection device of the utility model.

[0022] Figure 2 It is a structural schematic diagram of the sampling module, the reaction module and the color development module of the utility model.

[0023] In the figure, the sampling module 1, the sampling part 101, the wipe sample container 111, the wipe sample container discharge port 1111, the ultrasonic oscillation device 1112, the gas-liquid sampling part 102, the collection pump 121, the gas collecting pipe 122, the liquid collecting pipe 123, the collector 124, the extraction part 103, the extraction liquid container 131, the first diaphragm pump 132, the reaction module 2, the reaction container 201, the reaction container discharge port 211, the stirring part 212, the color changing part 202, the color changing agent container 221, the second diaphragm pump 222, the cleaning and degassing module 3, the detection module 4, the spectrophotometer 401, the waste liquid collection module 5, the third diaphragm pump 6 and the fourth diaphragm pump 7. DETAILED DESCRIPTION

[0024] The present invention will be further described below in conjunction with the embodiments.

[0025] Example:

[0026] like Figure 1 As shown, a residual hydrogen peroxide sampling and detection device comprises a sampling module 1, a reaction module 2, a cleaning and degassing module 3, a detection module 4 and a waste liquid collection module 5 which are sequentially connected by a hose, the waste liquid collection module is a waste liquid collection container, the waste liquid collection container is respectively connected to a wiping sample container, a reaction container and a detection module, the cleaning module is a cleaning and degassing machine, the cleaning and degassing machine is respectively connected to a wiping sample container, a reaction container and a detection module; the hose material can be selected from one of polytetrafluoroethylene and vinylidene fluoride;

[0027] The detection module is a spectrophotometer 401, which includes two LED light sources, two light-transmitting pools, two photosensitive elements and a display component. The light-transmitting pool is provided with a light-transmitting pool drainage port connected to the waste liquid collection module. The LED generates light of a fixed wavelength that passes through the light-transmitting pool and is incident on the sensory element. The photosensitive element can be selected from one of a CCD image sensor and a CMOS sensor. The light-transmitting pool is made of quartz glass. The top of the light-transmitting pool is connected to the color-changing agent container through a hose. The hose can inject the color-changed test solution into the light-transmitting pool. The light-transmitting pool drainage port is connected to the waste liquid collection module. After the detection is completed, the test sample is discharged into the waste liquid collection module, and the cleaning liquid is discharged into the waste liquid collection module after cleaning. The display component includes an information processing circuit, a main control chip, a display screen, a power supply and a button. The signal processing circuit is electrically connected to the photosensitive element and the main control chip, and the display screen, the power supply and the button are electrically connected to the main control chip. The waste liquid collector is also connected to the wiping sample container drainage port, the reaction container drainage port and the light-transmitting pool drainage port.

[0028] like Figure 2 As shown, the sampling module includes a wipe sampling part 101, a gas-liquid sampling part 102 and an extraction part 103. The wipe sampling part includes a wipe sample container 111. The wipe sample container is made of glass. The wipe sample container is used to load a wipe swab and small consumables. The wipe sample container is provided with a protective cover arranged on the wipe sample container. The wipe sample container is provided with a wipe sample container discharge port 1111 and an ultrasonic oscillation device 1112 detachably connected to the wipe sample container. The ultrasonic oscillation device is an ultrasonic oscillator; the gas-liquid sampling part includes a collection pump 121, a gas collecting pipe 122 located on the collection pump, and a liquid collecting pipe 1112. 23 and a collector 124 connected to a collecting pump, the collector is connected to the wiping sample container by a hose; the collector is a glass container, and a metering scale is provided on the collector; the gas collecting pipe and the liquid collecting pipe are made of glass; the extraction part includes an extraction liquid container 131 and a first diaphragm pump 132, the first diaphragm pump is connected to the wiping sample container and the extraction liquid container by a hose, the extraction liquid container is provided with hydrogen peroxide extraction liquid, and the extraction liquid container is a glass bottle; the first diaphragm pump is provided with a microfiltration membrane, the microfiltration membrane is a polytetrafluoroethylene microporous filter membrane with a pore size of 0.22μm, and the wiping sample container is connected to the reaction container by a third diaphragm pump 6;

[0029] The reaction module includes a reaction container 201 and a color-changing member 202 connected to the reaction container. The reaction container is provided with a stirring member 212 detachably connected to the reaction container and a reaction container discharge port 211. The stirring member includes a magnetic stirrer and a magnetic rotor. The magnetic rotor is located in the reaction container and is a polytetrafluoroethylene magnetic rotor. The color-changing member includes a color-changing agent container 221 and a second diaphragm pump 222. The second diaphragm pump is connected to the color-changing reaction container by a hose. The second diaphragm pump is provided with a microfiltration membrane, which is a polytetrafluoroethylene microporous filter membrane with a pore size of 0.22 μm. The reaction container is connected to the detection module by a fourth diaphragm pump 7.

[0030] The application process of the residual hydrogen peroxide sampling and detection device includes standard curve testing and sample testing.

[0031] The standard curve test steps include: injecting the standard solution into the wipe sample container, starting the first diaphragm pump to inject the extract into the wipe sample container to mix with the standard solution, starting the ultrasonic oscillator to extract for 15 minutes, turning off the ultrasonic oscillation device, passing the extract into the reaction container through the third diaphragm pump, starting the second diaphragm pump to inject the color-changing agent into the reaction container for color change reaction, starting the magnetic stirrer during the reaction, and injecting a fixed volume of color-developing solution into the light-transmitting cell of the spectrophotometer through the fourth diaphragm pump after the reaction is completed. After the test is completed, the test liquid in the light-transmitting cell is discharged into the waste liquid collector, and starting Start the cleaning degasser to clean the sampling module, reaction module and detection module. The cleaning liquid is injected into the waste liquid collector from the drain port of the wipe sample container, the drain port of the reaction container and the drain port of the light-transmitting pool to complete a standard sample test. When the next gradient standard solution test is carried out, the wipe sample container needs to be rinsed with the extraction liquid. The standard solution is tested in 5 concentration gradients. The test structure generates a fitting curve through the software. The fitting curve needs to judge the linear correlation. If R≥0.99, the parameters are saved for future use. If R<0.99, the standard curve is not saved and the standard curve test needs to be carried out again.

[0032] The sample testing steps include:

[0033] Solid consumable sample or wipe sample detection: place the solid consumable sample or wipe sample in a wipe sample container, start the first diaphragm pump to inject the extract into the wipe sample container to mix with the standard solution, start the ultrasonic oscillator for extraction for 30 minutes, turn off the ultrasonic oscillation device after the extraction is completed, pass the extract into the reaction container through the third diaphragm pump, and then start the second diaphragm pump to inject the color-changing agent into the reaction container for color change reaction, turn on the magnetic stirrer during the reaction, and after the reaction is completed, inject the color-developing solution into the light-transmitting cell of the spectrophotometer through the fourth diaphragm pump for detection, input the standard curve to calculate the residual hydrogen peroxide content in the test sample, and after the test is completed, discharge the test liquid in the light-transmitting cell, the excess reaction solution in the reaction container, and the excess waste liquid in the wipe sample container into the waste liquid collector, then start the cleaning degasser to clean the sampling module, the reaction module and the detection module, and inject the cleaning liquid into the waste liquid collector to complete the residual hydrogen peroxide content detection of the solid consumable sample or wipe sample.

[0034] Gas sample detection: First, start cleaning the degassing device to perform vacuum degassing on the device, then turn on the first diaphragm pump to inject the extract into the wipe sample container, then turn on the collecting pump to collect the gas into the collector through the gas collecting pipe, then pass the liquid in the collector into the extract and turn on the ultrasonic oscillation device, extract for 1 hour, after the extraction is completed, inject a fixed volume of extract into the reaction container through the third diaphragm pump, then start the second diaphragm pump to inject the color-changing agent into the reaction container for color change reaction, turn on the magnetic stirrer during the reaction, after the reaction is completed, inject the color-developing solution into the light-transmitting cell of the spectrophotometer through the fourth diaphragm pump for detection, input the standard curve to calculate the residual hydrogen peroxide content in the gas test sample, after the test is completed, discharge the test liquid in the light-transmitting cell, the excess reaction solution in the reaction container and the excess waste liquid in the wipe sample container into the waste liquid collector, then turn on the cleaning pump to clean the sampling module, the reaction module and the detection module, and inject the cleaning liquid into the waste liquid collector to complete the residual hydrogen peroxide content detection of the gas sample.

[0035] Liquid sample detection: Turn on the collection pump to inject the liquid sample into the collector through the liquid collecting pipe, and then inject the liquid in the collector into the wipe sample container, turn on the first diaphragm pump to inject the extract into the wipe sample container, turn on the ultrasonic oscillator to extract for 20 minutes, turn off the ultrasonic oscillation device after the extraction is completed, pass the extract into the reaction container through the third diaphragm pump, and then turn on the second diaphragm pump to inject the color-changing agent into the reaction container for color change reaction, turn on the magnetic stirrer during the reaction, and after the reaction is completed, inject the color-developing solution into the light-transmitting cell of the spectrophotometer through the fourth diaphragm pump for detection, input the standard curve to calculate the residual hydrogen peroxide content in the test sample, and after the test is completed, discharge the test liquid in the light-transmitting cell, the excess reaction solution in the reaction container, and the excess waste liquid in the wipe sample container into the waste liquid collector, and then turn on the cleaning pump to clean the sampling module, reaction module and detection module, and inject the cleaning liquid into the waste liquid collector to complete the residual hydrogen peroxide content detection of the liquid sample.

[0036] The spectrophotometer of the residual hydrogen peroxide sampling and detection device is provided with two sets of ultraviolet light detection components, which can perform extraction and color development reaction of other samples while performing spectrophotometric detection on one sample, and can perform rapid detection of multiple samples at the same time, which can significantly improve the detection efficiency.

[0037] The above-mentioned residual hydrogen peroxide sampling and detection device can use a variety of reagents for hydrogen peroxide spectrophotometric colorimetric method; when titanyl sulfate is selected as the reagent, the titanyl sulfate solution can be used as an extracting liquid and a color changing agent at the same time, and hydrogen peroxide can directly change color after contacting titanyl sulfate, so a color changing reaction can occur during the extraction process. In the device of the utility model, the two steps of extraction and color changing can be completed directly in the wipe sample container, and the color changing agent in the wipe sample container can be directly introduced into the spectrophotometer for testing; when testing the low-concentration hydrogen peroxide content of 3,3,5,5-tetramethylbenzidine and catalase mechanisms, an aqueous solution or an aqueous solution with a stabilizer added is selected as the extracting agent, and the color changing agent is a buffer, 3,3,5,5-tetramethylbenzidine, a catalase peroxidase stabilizer and a 3,3,5,5-tetramethylbenzidine stabilizer; the same is applicable to the test of other hydrogen peroxide color changing mechanisms, and the wavelength of the spectrophotometer needs to be adapted to the color changing mechanism.

Claims

1. A residual hydrogen peroxide sampling and detection device, characterized in that it includes A sampling module (1), a reaction module (2), a cleaning and degassing module (3), a detection module (4) and a waste liquid collection module (5) are sequentially connected, the sampling module comprising a wiping sampling component (101) and a gas-liquid sampling component (102) and an extraction component (103) connected to the wiping sampling component, the wiping sampling component comprising a wiping sample container (111); the gas-liquid sampling component comprising a collection pump (121) and a gas collecting pipe (122) and a liquid collecting pipe (123) on the collection pump, and a collector (124) connected to the wiping sample container; the reaction module comprises a reaction container (201) connected to the cleaning and degassing module and a color changing component (202) connected to the reaction container, and the detection module is a spectrophotometer (401).

2. A residual hydrogen peroxide sampling and detection device according to claim 1, characterized in that: The wipe sample container is provided with a wipe sample container liquid discharge port (1111).

3. A residual hydrogen peroxide sampling and detection device according to claim 1 or 2, characterized in that: The wipe sample container is provided with an ultrasonic oscillation device (1112) which is detachably connected to the wipe sample container.

4. The residual hydrogen peroxide sampling and detection device according to claim 1 is characterized in that: The gas collecting pipe is made of glass, and the liquid collecting pipe is made of glass.

5. The residual hydrogen peroxide sampling and detection device according to claim 1 is characterized in that: The extraction component comprises an extraction liquid container (131) and a first diaphragm pump (132) connected to the extraction liquid container and the wipe sample container.

6. The residual hydrogen peroxide sampling and detection device according to claim 1 is characterized in that: The reaction container is provided with a reaction container liquid discharge port (211).

7. A residual hydrogen peroxide sampling and detection device according to claim 1 or 6, characterized in that: The reaction container is also provided with a stirring member (212) detachably connected to the reaction container, wherein the stirring member comprises a magnetic stirrer and a magnetic rotor located in the reaction container.

8. The residual hydrogen peroxide sampling and detection device according to claim 1 is characterized in that: The color changing component comprises a color changing agent container (221) and a second diaphragm pump (222) connected to the color changing agent container and the reaction container.

9. The residual hydrogen peroxide sampling and detection device according to claim 1 is characterized in that: The waste liquid collection module is a waste liquid collection container, and the waste liquid collection container is connected to the sampling module, the reaction module and the detection module.

10. The residual hydrogen peroxide sampling and detection device according to claim 1, characterized in that: The cleaning and degassing module comprises a degassing cleaning machine, and the degassing cleaning machine is connected to a sampling module, a reaction module and a detection module.

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