Antibody concentration device

Abstract

The utility model discloses an antibody concentration device, comprising an upper pipe, the bottom of which is welded to fix an upper flange, and the bottom of which is installed with a lower flange, while a concentration cylinder is welded to fix the bottom of the lower flange, and a support is fixedly arranged on the upper side of the inner part of the concentration cylinder, and a ceramic filter element is positioned and installed on the surface of the support; a screw pipe is arranged at the bottom of the concentration cylinder, and the screw pipe is adapted to the size of a positioning seat, and the inner part of the positioning seat is screwed to fix the screw pipe. The antibody concentration device presses down a piston, and under the action of pressure, spherical monoclonal antibodies with a size greater than 10nm are retained on the surface of the ceramic filter element, and serum with a size less than 10nm is collected in the interior of a collection box, completing the separation work between monoclonal antibodies and serum, and achieving the purpose of concentrating monoclonal antibodies. When physical liquid addition filtration is adopted, the monoclonal antibodies are concentrated, and in the process of concentration, no byproducts are generated, and no loss of antibodies is caused.

Description

Technical Field

[0001] The utility model relates to the field of concentration equipment, in particular to antibody concentration equipment. Background Art

[0002] Antibodies are protein molecules produced by the immune system;

[0003] Specifically, when the body is stimulated by antigens (such as bacteria, viruses, foreign bodies, etc.), the B lymphocytes in the immune system will be activated and proliferate and differentiate, producing antibodies that can specifically recognize and bind to the antigen;

[0004] Antibodies play an extremely important role in immune defense, immune regulation, disease diagnosis and treatment. For example, in disease diagnosis, specific antibodies are used to detect the presence of antigens. In immunotherapy, monoclonal antibodies can be used to target tumors and other diseases.

[0005] Many types of antibodies can be concentrated by ultrafiltration, here are some common examples:

[0006] Monoclonal antibodies: such as monoclonal antibodies against specific targets used for disease diagnosis and treatment;

[0007] Polyclonal antibodies: For example, polyclonal antibodies raised against various antigens;

[0008] Taking monoclonal antibodies as an example, monoclonal antibodies are spherical and about 10nm in size. When cultured cells produce monoclonal antibodies, serum is present and is generally removed by salting out. Specifically, the antibodies are precipitated by adding appropriate salts, such as ammonium sulfate, while some impurities in the serum remain in the solution, and the precipitate is then collected by centrifugation and dissolved. A certain amount of antibodies may be precipitated at the same time during the salting out process, resulting in antibody loss. Utility Model Content

[0009] The purpose of the utility model is to provide an antibody concentration device to solve the defects mentioned in the above background technology.

[0010] To achieve the above object, an antibody concentration device is provided, comprising an upper pipe, an upper flange is welded and fixed at the bottom of the upper pipe, and a lower flange is installed at the bottom of the upper flange, a concentration cylinder is welded and fixed at the bottom of the lower flange, and a support is fixedly arranged on the upper side of the inner part of the concentration cylinder, and a ceramic filter element is positioned and installed on the surface of the support;

[0011] A screw pipe is arranged at the bottom of the concentrator, and the screw pipe is matched with the size of the positioning seat. Meanwhile, the screw pipe is fixed by screw connection inside the positioning seat, and a collecting box is fixedly installed at the bottom of the positioning seat.

[0012] Preferably, the upper connecting pipe is adapted to the size of the piston, and the piston is slidably disposed inside the upper connecting pipe, and the piston is a cylindrical structure made of rubber material.

[0013] Preferably, a connecting column is fixedly mounted on the top of the piston, and the axial cross-section of the piston and the upper connecting pipe is a concentric circle structure.

[0014] Preferably, the upper flange and the lower flange have the same size, and a sealing gasket is arranged between the upper flange and the lower flange. The upper flange and the lower flange are fixed by four sets of bolts, and the upper connecting pipe and the concentrating cylinder are fixedly connected by the upper flange and the lower flange.

[0015] Preferably, the ceramic filter press is a disc-shaped structure, and multiple groups of filter holes are evenly arranged on the ceramic filter press, and the pore size of the filter holes is less than 10nm, and multiple groups of exhaust holes are evenly arranged on the outer side of the concentration cylinder.

[0016] Preferably, the upper connecting pipe, the concentrating cylinder, the positioning seat and the collecting box are all cylindrical structures, and a limiting piece is fixedly installed on the outer side of the concentrating cylinder, and the diameter of the limiting piece is larger than the diameter of the positioning seat.

[0017] Compared with the prior art, the utility model has the following beneficial effects: the piston is pressed down, and under the action of pressure, spherical monoclonal antibodies with a size larger than 10 nm are retained on the surface of the ceramic filter element, and serum with a size smaller than 10 nm is collected inside the collection box, thereby completing the separation work between the monoclonal antibodies and the serum, and achieving the purpose of concentrating the monoclonal antibodies. When physical liquid addition filtration is adopted, the monoclonal antibodies are concentrated, and in the process of concentration, no by-products are generated and no loss of antibodies is caused. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of the front view of the structure of the utility model;

[0019] Figure 2 The utility model structure Figure 1 Side view of

[0020] Figure 3 The utility model structure Figure 1 A top view of

[0021] Figure 4 The utility model structure Figure 1 Bottom view of .

[0022] Numbers in the figure: 1. Connecting column; 2. Piston; 3. Upper connecting pipe; 4. Upper flange; 5. Sealing gasket; 6. Lower flange; 7. Concentrator; 8. Support platform; 9. Ceramic filter element; 10. Limiting plate; 11. Screw connecting pipe; 12. Positioning seat; 13. Collection box. DETAILED DESCRIPTION

[0023] See also Figure 1-4 The utility model provides an antibody concentration device, comprising an upper pipe 3, an upper flange 4 is welded and fixed to the bottom of the upper pipe 3, and a lower flange 6 is installed at the bottom of the upper flange 4, and a concentration cylinder 7 is welded and fixed to the bottom of the lower flange 6, and a support 8 is fixedly arranged on the upper side of the inner part of the concentration cylinder 7, and a ceramic filter element 9 is positioned and installed on the surface of the support 8;

[0024] A screw tube 11 is provided at the bottom of the concentrator cylinder 7 , and the screw tube 11 matches the size of the positioning seat 12 . The screw tube 11 is screwed and fixed inside the positioning seat 12 , and a collection box 13 is fixedly installed at the bottom of the positioning seat 12 .

[0025] Working principle: When in use, the monoclonal antibody and serum mixture is placed inside the upper pipe 3, and the piston 2 is pressed down. Under the action of pressure, spherical monoclonal antibodies with a size greater than 10nm are retained on the surface of the ceramic filter element 9, and serum with a size less than 10nm is collected inside the collection box 13, completing the separation between the monoclonal antibody and the serum, and achieving the purpose of concentrating the monoclonal antibody. When physical liquid addition filtration is used, the monoclonal antibody is concentrated. During the concentration process, no by-products are produced and no loss of antibodies is caused.

[0026] As a preferred embodiment, the upper pipe 3 is adapted to the size of the piston 2, and the piston 2 is slidably disposed inside the upper pipe 3, and the piston 2 is a cylindrical structure made of rubber material.

[0027] like Figure 1-4 As shown: the connecting column 1 is fixedly installed on the top of the piston 2, and the axial section of the piston 2 and the upper connecting pipe 3 is a concentric circle structure.

[0028] A connecting column 1 is mounted on the piston 2. The connecting column 1 can be driven manually or by an external cylinder, depending on the usage.

[0029] As a preferred embodiment, the upper flange 4 and the lower flange 6 have the same size, and a sealing gasket 5 is arranged between the upper flange 4 and the lower flange 6. At the same time, the upper flange 4 and the lower flange 6 are fixed by four sets of bolts, and the upper connecting pipe 3 and the concentration cylinder 7 are fixedly connected by the upper flange 4 and the lower flange 6.

[0030] The ceramic filter press 9 is a disc-shaped structure, and multiple groups of filter holes are evenly arranged on the ceramic filter press 9. The pore size of the filter holes is less than 10 nm. Multiple groups of exhaust holes are evenly arranged on the outer side of the concentration cylinder 7.

[0031] like Figure 1-4As shown: the ceramic filter press 9 is a disc-shaped structure, on which a large number of filter holes with a pore size of 10nm are arranged. Under the action of pressure, monoclonal antibodies larger than 10nm are retained on the surface of the ceramic filter press 9, and serum and other substances smaller than 10nm are collected inside the collection box 13. By separating the upper pipe 3 and the concentration cylinder 7, it is convenient to remove the concentrated antibodies from the surface of the ceramic filter press 9.

[0032] The upper connecting pipe 3 , the concentrating cylinder 7 , the positioning seat 12 and the collecting box 13 are all cylindrical structures, and a limiting piece 10 is fixedly installed on the outer side of the concentrating cylinder 7 , and the diameter of the limiting piece 10 is greater than the diameter of the positioning seat 12 .

Claims

1. An antibody concentration device, comprising an upper pipe (3), characterized in that: The upper flange (4) is welded and fixed to the bottom of the upper pipe (3), and the lower flange (6) is installed on the bottom of the upper flange (4). At the same time, the concentrator (7) is welded and fixed to the bottom of the lower flange (6), and a support (8) is fixedly arranged on the upper side of the inner part of the concentrator (7), and a ceramic filter element (9) is positioned and installed on the surface of the support (8); A screw pipe (11) is provided at the bottom of the concentration cylinder (7), and the screw pipe (11) matches the size of the positioning seat (12). The screw pipe (11) is screwed and fixed inside the positioning seat (12), and a collection box (13) is fixedly installed at the bottom of the positioning seat (12).

2. The antibody concentration device according to claim 1, characterized in that: The upper pipe (3) is adapted to the size of the piston (2), and the piston (2) is slidably arranged inside the upper pipe (3), while the piston (2) is a cylindrical structure made of rubber material.

3. An antibody concentration device according to claim 2, characterized in that: The connecting column (1) is fixedly mounted on the top of the piston (2), and the axial cross-sections of the piston (2) and the upper connecting pipe (3) are concentric circle structures.

4. The antibody concentration device according to claim 1, characterized in that: The upper flange (4) and the lower flange (6) have the same size, and a sealing gasket (5) is provided between the upper flange (4) and the lower flange (6). The upper flange (4) and the lower flange (6) are fixed by four sets of bolts, and the upper pipe (3) and the concentrating cylinder (7) are fixedly connected by the upper flange (4) and the lower flange (6).

5. The antibody concentration device according to claim 1, characterized in that: The ceramic filter press (9) is a disc-shaped structure, and a plurality of groups of filter holes are evenly arranged on the ceramic filter press (9), and the pore diameter of the filter holes is less than 10 nm. A plurality of groups of exhaust holes are evenly arranged on the outer side of the concentration cylinder (7).

6. The antibody concentration device according to claim 1, characterized in that: The upper pipe (3), the concentrating cylinder (7), the positioning seat (12) and the collecting box (13) are all cylindrical structures, and a limiting plate (10) is fixedly installed on the outer side of the concentrating cylinder (7), and the diameter of the limiting plate (10) is greater than the diameter of the positioning seat (12).

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