A production-type continuous flow peptide synthesis integrated column

Abstract

The utility model relates to the technical field of polypeptide synthesis, and discloses a production-type continuous-flow polypeptide synthesis integrated column, including a positioning device, a supporting device is provided at the lower end of the positioning device, the positioning device includes a base, a plurality of bottom boxes are fixedly connected to the upper end of the base, the four corners of the upper end of the base are fixedly connected to fixed columns, the upper end of the fixed column is hinged with a support rod, the outer surfaces of both ends of the support rod are fixedly connected with a clamping block, and the upper end of the support rod is provided with a limit plate. The production-type continuous-flow polypeptide synthesis integrated column is achieved by erecting the support rod, using the clamping block to limit the limit plate, tightening the nut with the support rod thread to fix the limit plate, passing the integrated column through the limit plate and clamping it into the bottom box, then putting on a sleeve and rotating it, so that the sleeve and the limit plate thread rotate to position the integrated column, thereby achieving the effect of easy disassembly and assembly of the integrated column and convenient transportation.

Description

Technical Field

[0001] The utility model relates to the technical field of polypeptide synthesis, in particular to a production-type continuous-flow polypeptide synthesis integrated column. Background Art

[0002] At present, the chemical synthesis of peptides mainly includes three methods: intermittent solid phase synthesis, liquid phase synthesis, and liquid-solid phase combination. The intermittent solid phase synthesis method has problems such as large amount of protected amino acids, large solvent consumption, solvent recovery as a mixture in the coupling process, and long peptide bond coupling time. Liquid phase synthesis has the advantage of large-scale production for medium and short peptides, but it is not suitable for long peptide synthesis, especially because the production process is complex, labor-intensive, long cycle, and low efficiency.

[0003] According to a production-type continuous flow peptide synthesis integrated column disclosed in Chinese patent publication number CN 217614684 U, by replacing a single large synthesis column with an integrated column with the same reaction volume, the difficulty of manufacturing the large synthesis column, the negative effects of peptide synthesis such as temperature rise, side reactions, and reaction control difficulties are avoided; secondly, the integrated column is used, and the fluid distribution in the column is better than that of the large synthesis column, and the flow rate and flow rate of the reaction material can be controlled, resulting in high production efficiency. However, the device is difficult to disassemble and assemble in actual operation, and is not convenient for storage and transportation.

[0004] To this end, we proposed a production-type continuous flow peptide synthesis integrated column to solve the problem that the above-mentioned device is difficult to disassemble and transport. Utility Model Content

[0005] The utility model aims to provide a production-type continuous-flow peptide synthesis integrated column to solve the problem that the above-mentioned device is difficult to disassemble and assemble and inconvenient to carry in the above-mentioned background technology.

[0006] In order to solve the above technical problems, the utility model provides the following technical solutions: a production-type continuous flow polypeptide synthesis integrated column, comprising a positioning device, wherein a supporting device is provided at the lower end of the positioning device;

[0007] The positioning device includes a base, a plurality of bottom boxes are fixedly connected to the upper end of the base, fixing columns are fixedly connected to the four corners of the upper end of the base, a support rod is hinged on the upper end of the fixing column, blocks are fixedly connected to the outer surfaces of both ends of the support rod, a limit plate is provided on the upper end of the support rod, a sleeve matching the bottom box is threadedly connected to the outer surface of the limit plate, a nut is threadedly connected to the outer surface of the support rod, and an integrated column is provided between the bottom box and the sleeve.

[0008] Preferably, the supporting device includes a frame, a pillar is fixedly connected to the right side of the lower end of the frame, a sliding sleeve is hinged to the left side of the lower end of the frame, the lower end of the sliding sleeve is rotatably connected to a swivel, an extension rod is threadedly connected to the inner surface of the swivel, the extension rod and the lower end of the pillar are hinged with a pad, a positioning plate is fixedly connected to the right side of the lower end of the frame, screw holes are provided on the pad and the positioning plate, a cylinder is hinged to the middle part of the frame, a push rod is slidably connected to the other end of the cylinder, and a locking rod is threadedly connected to one end of the cylinder close to the push rod.

[0009] Preferably, the support rod is configured as an L-shaped structure, and the four fixing columns are staggered and distributed at the four corners of the base.

[0010] Preferably, the base box and the sleeve are both provided with through holes, and an opening is provided at a corner of the base box and the sleeve on the facing sides.

[0011] Preferably, the frame is fixedly connected to the base, and the extension rod is slidably connected to the sliding sleeve.

[0012] Preferably, the other end of the push rod is hinged to the sliding sleeve, and the cushion block on the left side is provided with an inclined surface.

[0013] Preferably, recessed holes adapted to the locking rod are provided on both sides of the front end of the push rod, and the support column and the sliding sleeve are staggered and distributed on both sides of the frame.

[0014] Compared with the prior art, the beneficial effects achieved by the utility model are:

[0015] First, the utility model erects the support rod, uses the clamping block to limit the limit plate, and fixes the limit plate by tightening the nut and the support rod thread. The integrated column is inserted into the bottom box through the limit plate, and then the sleeve is put on and rotated to rotate the sleeve and the limit plate thread to position the integrated column, thereby achieving the effect of easy disassembly and assembly of the integrated column and convenient transportation.

[0016] Second, the utility model supports the equipment through the pillar, the sliding sleeve and the extension rod. The push rod is slid to retract the push rod into the cylinder, and the locking operation is performed through the locking rod. The push rod drives the sliding sleeve to rotate to tilt the sliding sleeve, and the swivel is rotated to make the swivel drive the extension rod to extend, so that the pad at the lower end of the extension rod is close to the pillar at the right end, so that the four pads and the positioning plate are located in the same vertical plane, and the bolts are passed through the screw holes to lock them with the wall, so that the equipment can be hung on the wall, thereby achieving the effect of facilitating the equipment to support the ground and the wall, and improving the practicality. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a three-dimensional schematic diagram of the structure of the utility model;

[0018] Figure 2The structure of the utility model Figure 1 The enlarged schematic diagram of part A in the middle;

[0019] Figure 3 It is a cross-sectional schematic diagram of the structural sleeve of the utility model;

[0020] Figure 4 It is a three-dimensional schematic diagram of the structural support device of the utility model.

[0021] Wherein: 1. Positioning device; 101. Base; 102. Bottom box; 103. Fixed column; 104. Support rod; 105. Block; 106. Limit plate; 107. Sleeve; 108. Nut; 109. Integrated column; 2. Support device; 201. Frame; 202. Pillar; 203. Sliding sleeve; 204. Swivel; 205. Extension rod; 206. Pad block; 207. Positioning plate; 208. Cylinder; 209. Push rod; 210. Locking rod. DETAILED DESCRIPTION

[0022] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0023] The utility model provides the following technical solutions:

[0024] Embodiment 1

[0025] See also Figure 1-3 , a production-type continuous flow peptide synthesis integrated column, comprising a positioning device 1, a supporting device 2 is provided at the lower end of the positioning device 1;

[0026] The positioning device 1 includes a base 101, and a plurality of bottom boxes 102 are fixedly connected to the upper end of the base 101. Fixed columns 103 are fixedly connected to the four corners of the upper end of the base 101. The upper end of the fixed column 103 is hinged with a support rod 104. The outer surfaces of both ends of the support rod 104 are fixedly connected with a block 105. The upper end of the support rod 104 is provided with a limit plate 106. The outer surface of the limit plate 106 is threadedly connected with a sleeve 107 matching the bottom box 102. The outer surface of the support rod 104 is threadedly connected with a nut 108. An integrated column 109 is provided between the bottom box 102 and the sleeve 107.

[0027] Through the above technical solution, by erecting the support rod 104, using the block 105 to limit and support the limit plate 106, using the nut 108 to tighten the thread on the support rod 104 to fix the limit plate 106, the integrated column 109 is passed through the limit plate 106 and inserted into the bottom box 102, and then the sleeve 107 is put on and rotated to make the sleeve 107 and the limit plate 106 threadedly rotate to position the integrated column 109, thereby achieving the effect of easy disassembly and assembly of the integrated column and convenient transportation.

[0028] Specifically, the support rod 104 is configured as an L-shaped structure, and the four fixing columns 103 are staggered and distributed at the four corners of the base 101 .

[0029] Through the above technical solution, the support rods 104 are staggered and distributed, which is convenient for staggered folding. The limiting plate 106 is positioned through the protrusions at the lower ends of the support rods 104, thereby reducing the volume and facilitating transportation.

[0030] Specifically, both the bottom box 102 and the sleeve 107 are provided with through holes, and an opening is provided at a corner of the bottom box 102 and the sleeve 107 facing each other.

[0031] Through the above technical solution, the base box 102 and the sleeve 107 open through holes to facilitate the connection of the integrated column 109 with the main pipeline for diversion and collection, so that the flow rate and flow of the reaction materials can be controlled, bringing about a high production efficiency effect. The openings opened in the base box 102 and the sleeve 107 limit the integrated column 109.

[0032] Embodiment 2

[0033] See also Figure 1 , Figure 4 , and on the basis of Example 1, it is further obtained that: the supporting device 2 includes a frame 201, a pillar 202 is fixedly connected to the right side of the lower end of the frame 201, a sliding sleeve 203 is hinged on the left side of the lower end of the frame 201, the lower end of the sliding sleeve 203 is rotatably connected to a swivel 204, the inner surface of the swivel 204 is threadedly connected to an extension rod 205, the extension rod 205 and the lower end of the pillar 202 are both hinged with a cushion block 206, a positioning plate 207 is fixedly connected to the right side of the lower end of the frame 201, screw holes are provided on the cushion block 206 and the positioning plate 207, a cylinder 208 is hinged on the middle part of the frame 201, the other end of the cylinder 208 is slidably connected to a push rod 209, and the end of the cylinder 208 close to the push rod 209 is threadedly connected to a locking rod 210.

[0034] Through the above technical scheme, the equipment is supported by the pillar 202, the sleeve 203 and the extension rod 205, the push rod 209 is slid to retract the push rod 209 into the cylinder 208, and the locking operation is performed through the locking rod 210. The push rod 209 drives the sleeve 203 to rotate, so that the sleeve 203 is tilted, and the rotating ring 204 is rotated to make the rotating ring 204 drive the extension rod 205 to extend, so that the pad 206 at the lower end of the extension rod 205 is close to the pillar 202 at the right end, so that the four pads 206 and the positioning plate 207 are located in the same vertical plane, and the bolts are passed through the screw holes to lock with the wall, so that the equipment can be hung on the wall, thereby achieving the effect of facilitating the equipment to support the ground and the wall, and improving the practicality.

[0035] Specifically, the frame 201 is fixedly connected to the base 101 , and the extension rod 205 is slidably connected to the sliding sleeve 203 .

[0036] Through the above technical solution, the frame 201 and the base 101 are fixedly connected by fastening bolts, and are detachable to increase the flexibility of use. The sliding sleeve 203 plays a limiting role on the extension rod 205.

[0037] Specifically, the other end of the push rod 209 is hinged to the sliding sleeve 203, and the left cushion block 206 is provided with an inclined surface.

[0038] Through the above technical solution, the push rod 209 supports the sliding sleeve 203, and the cushion block 206 is provided with an inclined surface to facilitate fitting with the wall.

[0039] Specifically, recessed holes adapted to the locking rod 210 are provided on both sides of the front end of the push rod 209 , and the support column 202 and the sliding sleeve 203 are staggered and distributed on both sides of the frame 201 .

[0040] Through the above technical solution, by providing two recessed holes on the push rod 209, the locking rod 210 can be easily positioned.

[0041] Although specific embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and variations may be made to these specific embodiments without departing from the principles and spirit thereof, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A production-type continuous flow peptide synthesis integrated column, comprising a positioning device (1), characterized in that: A supporting device (2) is provided at the lower end of the positioning device (1); The positioning device (1) comprises a base (101), the upper end of the base (101) is fixedly connected to a plurality of bottom boxes (102), the four corners of the upper end of the base (101) are fixedly connected to fixing columns (103), the upper ends of the fixing columns (103) are hinged to support rods (104), the outer surfaces of both ends of the support rods (104) are fixedly connected to clamping blocks (105), the upper ends of the support rods (104) are provided with limit plates (106), the outer surfaces of the limit plates (106) are threadedly connected to sleeves (107) matching the bottom boxes (102), the outer surfaces of the support rods (104) are threadedly connected to nuts (108), and an integrated column (109) is provided between the bottom boxes (102) and the sleeves (107).

2. A production-type continuous flow peptide synthesis integrated column according to claim 1, characterized in that: The support device (2) comprises a frame (201), the lower right side of the frame (201) is fixedly connected to a support (202), the lower left side of the frame (201) is hingedly connected to a sliding sleeve (203), the lower end of the sliding sleeve (203) is rotatably connected to a rotating ring (204), the inner surface of the rotating ring (204) is threadedly connected to an extension rod (205), and the extension rod (205) and the lower end of the support (202) are both hingedly connected. A cushion block (206) is connected, a positioning plate (207) is fixedly connected to the right side of the lower end of the frame (201), screw holes are provided on the cushion block (206) and the positioning plate (207), a cylinder (208) is hingedly connected to the middle of the frame (201), the other end of the cylinder (208) is slidably connected to a push rod (209), and one end of the cylinder (208) close to the push rod (209) is threadedly connected to a locking rod (210).

3. A production-type continuous flow peptide synthesis integrated column according to claim 1, characterized in that: The support rod (104) is configured as an L-shaped structure, and the four fixing columns (103) are staggered and distributed at the four corners of the base (101).

4. A production-type continuous flow peptide synthesis integrated column according to claim 1, characterized in that: The bottom box (102) and the sleeve (107) are both provided with through holes, and an opening is provided at a corner of the bottom box (102) and the sleeve (107) on the facing sides.

5. A production-type continuous flow peptide synthesis integrated column according to claim 2, characterized in that: The frame (201) is fixedly connected to the base (101), and the extension rod (205) is slidably connected to the sliding sleeve (203).

6. A production-type continuous flow peptide synthesis integrated column according to claim 2, characterized in that: The other end of the push rod (209) is hinged to the sliding sleeve (203), and the cushion block (206) on the left side is provided with an inclined surface.

7. A production-type continuous flow peptide synthesis integrated column according to claim 2, characterized in that: Concave holes matching the locking rod (210) are provided on both sides of the front end of the push rod (209), and the support (202) and the sliding sleeve (203) are staggered and distributed on both sides of the frame (201).

Images