Fluid system and sequencing device

Abstract

The utility model discloses a liquid circuit system and a sequencing device. The liquid circuit system includes a flow cell and a pump-valve assembly, the flow cell includes a plurality of fluid channels, each fluid channel is provided with an inlet and an outlet, the pump-valve assembly is used to draw a plurality of samples to be tested from a sample box, and pump each sample to be tested into the fluid channel from the outlet of a corresponding fluid channel; or, the pump-valve assembly is used to draw a single sample to be tested from the sample box, and pump the single sample to be tested into the fluid channel from the inlet of each fluid channel. In this way, different injection modes can be selected according to the number of samples to be tested, which improves the flexibility of loading the samples to be tested; in addition, by allowing each of the multiple samples to be tested to enter the fluid channel from the outlet of a corresponding fluid channel, cross contamination between the multiple samples to be tested is avoided, and the sequencing quality and sequencing efficiency are improved.

Description

Technical Field

[0001] The utility model relates to the field of gene sequencing, in particular to a liquid path system and a sequencing device. Background Art

[0002] Gene sequencing technology refers to the technical means of obtaining DNA or RNA base sequences through detection. The currently dominant sequencing technology is high-throughput sequencing technology. In the sequencing platform based on sequencing by synthesis to achieve high-throughput sequencing, the general process of gene sequencing includes fixing the nucleic acid sample to be tested on a flow cell in a manner such as hybridization; then using PCR amplification to form nucleic acid molecule clusters in the nucleic acid sample to be tested; then adding sequencing reagents, such as bases with fluorescent groups, polymerases, and primers, to the flow cell, and through the principle of base complementary pairing, the bases with fluorescent groups are combined with the bases on the nucleic acid sample to be tested; finally, the fluorescent group is excited to produce fluorescence through an optical imaging system and the fluorescence is collected to form an image, and base recognition is performed through the image, thereby realizing the base sequence determination of the nucleic acid sample to be tested.

[0003] In the prior art, high-throughput sequencing can achieve nucleic acid sequence determination of multiple samples on the same flow cell, which can be achieved through multiple sample analysis. Multiple sample analysis will add a label or index (index, barcode or tag) that uniquely corresponds to the sample from which the nucleic acid fragment comes to each nucleic acid fragment during library construction, so that the libraries of multiple samples can be mixed in a reaction system for sequencing to obtain sequencing data, and then the sequencing data can be assigned to the corresponding sample according to the label or index, thereby obtaining the sequencing data of each sample. However, it is usually difficult to avoid the allocation errors of labels or indexes between multiple libraries, so that the sequencing data from sample A is incorrectly assigned to another sample B, resulting in false positives or false negatives.

[0004] In addition, high-throughput sequencing can realize the determination of nucleic acid sequences of multiple samples on the same flow cell, and can also be realized by loading multiple sample shunt channels into the flow cell, and then performing subsequent sequencing. For example, outside the sequencer, manually, using tools such as pipette guns, multiple different samples are manually loaded into different flow channels of the flow cell, and then the flow cell is placed in the sequencer to sequence different samples. This method can avoid the problem of sequencing data allocation errors caused by label or index allocation errors, but this method is cumbersome to operate, increases sequencing time and sequencing costs, and easily causes cross contamination between samples (for example, caused by manual operation errors or pipette guns not being cleaned properly). In addition, the flexibility of sample loading is also low, the sequencing time is long, and the sequencing efficiency is low. Utility Model Content

[0005] The utility model provides a liquid path system and a sequencing device to at least solve one of the technical problems existing in the prior art.

[0006] An embodiment of the present application provides a liquid circuit system, which includes a flow pool and a pump-valve assembly. The flow pool includes multiple fluid channels, each fluid channel is provided with an inlet and an outlet, and the pump-valve assembly is used to draw multiple samples to be tested from a sample box, and pump each sample to be tested into the fluid channel from the outlet of a corresponding fluid channel; or, the pump-valve assembly is used to draw a single sample to be tested from the sample box, and pump the single sample to be tested into the fluid channel from the inlet of each fluid channel.

[0007] In this way, the pump-valve assembly can allow a single sample to be tested to enter the fluid channel from the inlet of each fluid channel, and can also allow each of a plurality of samples to be tested to enter the fluid channel from the outlet of a corresponding fluid channel, so that different injection methods can be selected according to the number of samples to be tested, thereby improving the flexibility of loading the samples to be tested; in addition, by allowing each of a plurality of samples to be tested to enter the fluid channel from the outlet of a corresponding fluid channel, cross-contamination between a plurality of samples to be tested is avoided, thereby improving sequencing quality and sequencing efficiency.

[0008] In some embodiments, the pump valve assembly includes a first flow path selection valve and a pump group, the first flow path selection valve and the pump group are both located downstream of the flow pool, the first flow path selection valve connects the pump group with the fluid channel, and / or connects the pump group with the sample box, the first flow path selection valve and the pump group are used to draw multiple samples to be tested from the sample box, and allow each sample to be tested to enter the fluid channel from the outlet of a corresponding fluid channel.

[0009] In this way, the sample box and each fluid channel can be connected through the first flow path selection valve to achieve control of multiple flow paths of samples to be tested.

[0010] In some embodiments, the liquid path system includes multiple cache areas, and the multiple cache areas are arranged in a one-to-one correspondence with the multiple fluid channels. The multiple cache areas are connected to a first flow path selection valve and a pump group. The first flow path selection valve and the pump group pump each sample to be tested to a corresponding cache area respectively, and pump the sample to be tested in each cache area into the fluid channel from the outlet of the corresponding fluid channel.

[0011] In this way, each cache area can store one of the multiple samples to be tested, avoiding cross contamination between the multiple samples to be tested. The first flow path selection valve can select the sample box and the fluid channel to be connected to the cache area, realize the control of the flow path switching of the multiple samples to be tested, and the reverse flow of the samples to be tested in the fluid channel increases the flexibility of loading the samples to be tested, and improves the sequencing quality and sequencing efficiency.

[0012] In some embodiments, the first flow path selection valve is provided with a plurality of first ports and a plurality of second ports, each first port is selectively connected to a second port, the second port is connected to the cache area, the liquid path system includes a plurality of first pipelines, the plurality of first pipelines are arranged in one-to-one correspondence with the plurality of first ports, and the two ends of each first pipeline are respectively connected to an outlet of a fluid channel and a first port.

[0013] In this way, the samples and / or reagents to be tested in each buffer area can flow from the second port and the first port into the corresponding fluid channel through the first pipeline, thereby realizing the transportation of the samples and / or reagents to be tested.

[0014] In some embodiments, the first flow path selection valve is provided with a plurality of third ports, each third port is selectively connected to a second port, the liquid path system includes a plurality of second pipelines, the plurality of second pipelines are arranged in a one-to-one correspondence with the plurality of third ports, the two ends of each second pipeline are respectively connected to a sample box and a third port, and each second pipeline is used to allow a sample to be tested in the sample box to pass through the first flow path selection valve and enter a corresponding cache area.

[0015] In this way, the samples to be tested in the sample box can flow from the multiple first ports into the multiple buffer areas through the multiple second pipelines, thereby realizing the transportation of the samples to be tested.

[0016] In certain embodiments, the first flow path selection valve is provided with a plurality of fourth ports, each fourth port being selectively connected to a second port, and the liquid path system comprises a third pipeline, the third pipeline being arranged in one-to-one correspondence with the plurality of fourth ports, and both ends of the third pipeline being respectively connected to the test kit and each fourth port, and the third pipeline being used to allow the reagent in the test kit to enter each cache area through the first flow path selection valve.

[0017] In this way, the reagents in the test kit can flow from the multiple fourth ports into the multiple buffer areas through the third pipeline, thereby realizing the transportation of the reagents.

[0018] In certain embodiments, the first flow path selection valve includes a plurality of communication grooves, and the second port is communicated with one of the first port, the third port, and the fourth port through the communication grooves.

[0019] In this way, the buffer area can be selectively connected to the outlet of the fluid channel, the sample box and the reagent box through the connecting groove, so as to realize the control of the flow path of the sample to be tested and / or the reagent.

[0020] In certain embodiments, the first flow path selection valve includes a stator and a rotor rotatably arranged with the stator, the stator is provided with a plurality of groups of ports, each group of ports includes a first port, a second port, a third port and a fourth port, and the rotor is provided with a communication groove.

[0021] In this way, by rotating the rotor, the communicating groove can selectively connect the buffer area with the outlet of the fluid channel, the sample box and the reagent box, thereby realizing the control of switching the flow path of the sample to be tested and / or the reagent.

[0022] In certain embodiments, the first port, the second port, the third port, and the fourth port are spaced apart along the circumference of the stator.

[0023] In this way, the first port, the second port, the third port and the fourth port are arranged at intervals along the circumferential direction of the stator, so that the second port is communicated with one of the first port, the third port and the fourth port through the communication groove.

[0024] In some embodiments, there are multiple communicating grooves, and the multiple communicating grooves are arranged at intervals along the circumference of the rotor.

[0025] In this way, multiple communication grooves can simultaneously connect two adjacent ports in multiple groups of ports, and multiple fluid paths can be controlled simultaneously, reducing the number of solenoid valves used, thereby reducing the cost and occupied space of the fluid path system.

[0026] In certain embodiments, the pump-valve assembly includes a second flow path selection valve, which is located upstream of the flow pool. The second flow path selection valve includes a common port and at least one connecting port, the common port is connected to one end of the third pipeline, and the connecting port is connected to the reagent kit. The second flow path selection valve is used for the pump-valve assembly to draw reagents from the reagent kit and pump the reagents to each cache area, and pump the reagents of each cache area into the fluid channel from the outlet of the corresponding fluid channel.

[0027] In this way, the second flow path selection valve can connect the reagent box and the third pipeline through the common port and the connecting port to achieve control of the reagent flow path.

[0028] In certain embodiments, the pump valve assembly is used to pump the sample to be tested and / or the reagent to the buffer area in a negative pressure driven manner.

[0029] In this way, the pump valve assembly can generate negative pressure to drive the sample to be tested and / or the reagent to move to the buffer area, thereby realizing the transportation of the sample to be tested and / or the reagent.

[0030] In certain embodiments, the pump-valve assembly is used to pump the sample to be tested and / or reagent in the buffer area from the outlet of the fluid channel into the fluid channel in a positive pressure-driven manner.

[0031] In this way, the pump valve assembly can generate positive pressure to drive the sample to be tested and / or the reagent to move from the buffer area to the fluid channel, thereby realizing the transportation of the sample to be tested and / or the reagent.

[0032] In certain embodiments, the second flow path selection valve is used for the pump valve assembly to draw reagents from the reagent cartridge and pump the reagents into the sample cartridge to mix with each sample to be tested in the sample cartridge.

[0033] In this way, each sample to be tested and the reagent can be mixed in the sample box before entering the fluid channel to process the sample to be tested, saving the time required for processing the sample to be tested before the machine, which is conducive to industrialization.

[0034] In certain embodiments, the pump valve assembly is used to pump the reagent to each buffer area, and pump the reagent in each buffer area into the sample box to mix with the corresponding sample to be tested in the sample box.

[0035] In this way, the buffer area can store the reagent, so that the pump valve assembly can pump the reagent from the reagent box through each buffer area into the sample box.

[0036] In some embodiments, the pump valve assembly is used to pump the mixture of each sample and reagent back to the corresponding buffer area, and pump the mixture of the sample and reagent in each buffer area into the fluid channel from the outlet of the corresponding fluid channel.

[0037] In this way, the cache area can store each mixture of the sample to be tested and the reagent separately, so that the pump valve assembly can pump each mixture of the sample to be tested and the reagent from the outlet of the corresponding fluid channel into the fluid channel through the corresponding cache area, thereby realizing the reverse flow of the mixture of the sample to be tested and the reagent in the fluid channel, increasing the flexibility of loading the sample to be tested, and at the same time avoiding cross contamination between the mixtures of multiple samples to be tested and reagents, thereby improving the sequencing quality and sequencing efficiency.

[0038] In certain embodiments, the pump valve assembly is used to pump the mixture of the sample to be tested and the reagent to the buffer area in a negative pressure driven manner.

[0039] In this way, the pump valve assembly can generate negative pressure to drive the mixed solution of the sample to be tested and the reagent to move to the buffer area, thereby realizing the transportation of the mixed solution of the sample to be tested and the reagent.

[0040] In certain embodiments, the pump-valve assembly is used to pump the mixture of the sample to be tested and the reagent in the buffer area from the outlet of the fluid channel into the fluid channel in a positive pressure-driven manner.

[0041] In this way, the pump valve assembly can generate positive pressure to drive the mixed solution of the sample to be tested and the reagent to move from the buffer area to the fluid channel, thereby realizing the transportation of the mixed solution of the sample to be tested and the reagent.

[0042] In certain embodiments, the pump-valve assembly includes a manifold assembly, which is located upstream of the flow pool, a second flow path selection valve connects the reagent cartridge with the manifold assembly, and / or connects the reagent cartridge with the first flow path selection valve, the manifold assembly connects a common port and each fluid channel of the flow pool, and the second flow path selection valve, the manifold assembly and the pump group are used to draw a single sample and / or reagent to be tested from the sample box and / or the reagent cartridge, and allow the single sample and / or reagent to be tested to enter the fluid channel from the inlet of each fluid channel.

[0043] In this way, the second flow path selection valve can select the manifold assembly and the first flow path selection valve to communicate with the reagent kit to achieve control of reagent flow path switching. In addition, the manifold assembly can divert a single sample to be tested and / or reagent so that the samples to be tested entering different fluid channels through the inlet of each fluid channel are the same sample to be tested or the same reagent.

[0044] An embodiment of the present application provides a sequencing device, comprising the above-mentioned liquid circuit system.

[0045] Additional aspects and advantages of the present invention will be given in part in the following description, and in part will become apparent from the following description, or will be learned through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0046] The above and / or additional aspects and advantages of the present invention will become apparent and easily understood from the description of the embodiments in conjunction with the following drawings, in which:

[0047] Figure 1 It is a structural schematic diagram of a liquid circuit system according to an embodiment of the utility model;

[0048] Figure 2 It is a schematic flow chart of the method of the embodiment of the utility model;

[0049] Figure 3 It is a schematic flow chart of the method of the embodiment of the utility model;

[0050] Figure 4 It is a structural schematic diagram of a first flow path selection valve in an embodiment of the utility model;

[0051] Figure 5 It is a structural schematic diagram of the first flow path selection valve in an embodiment of the utility model.

[0052] Explanation of the reference numerals: 100, liquid path system; 10, flow cell; 11, fluid channel; 12, inlet; 13, outlet; 20, pump valve assembly; 21, first flow path selection valve; 211, first port; 212, second port; 213, third port; 214, fourth port; 215, connecting groove; 216, stator; 217, rotor; 218, plug; 22, pump group; 23, second flow path selection valve; 231, common port; 232, connecting port; 24, manifold assembly; 30, sample box; 40, cache area; 50, reagent box; 60, waste liquid bottle; 70, solenoid valve; 81, first pipeline; 82, second pipeline; 83, third pipeline; 831, liquid inlet; 832, liquid outlet. DETAILED DESCRIPTION

[0053] The embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0054] The disclosure below provides many different embodiments or examples for realizing different structures of the utility model. In order to simplify the disclosure of the utility model, the components and settings of specific examples are described below. Of course, they are merely examples, and the purpose is not to limit the utility model. In addition, the utility model may repeat reference numbers and / or reference letters in different examples, and such repetition is for the purpose of simplification and clarity, and does not itself indicate the relationship between the various embodiments and / or settings discussed. In addition, the utility model provides various specific examples of processes and materials, but those of ordinary skill in the art may be aware of the application of other processes and / or the use of other materials.

[0055] In this article, "sequencing" refers to the determination of nucleic acid sequences, which is the same as "nucleic acid sequencing" or "gene sequencing", and refers to the determination of the order of bases in the primary structure of nucleic acid molecules, which can be achieved by synthetic sequencing (Sequencing by Synthesis, SBS), ligation sequencing (Sequencing by Ligation, SBL) or hybridization sequencing (SBH), etc. The so-called sequencing by synthesis includes not only the commonly understood SBS (typical ILLUMINA / Solexa technology) of using polymerase to catalyze the incorporation of nucleotides into the sample to be tested (synthesis reaction) and detecting the corresponding reaction signal to identify the type of the incorporated nucleotide, but also sequencing similar to SBS, which uses polymerase or non-polymerase to controllably introduce or connect nucleotides to the sample to be tested, and directly or indirectly detects the corresponding signal to determine the type of the connected nucleotide.

[0056] Sequencing may include DNA sequencing and / or RNA sequencing. It may include long-fragment sequencing and / or short-fragment sequencing, where the so-called long fragments and short fragments are relative, such as nucleic acid molecules longer than 1Kb, 2Kb, 5Kb or 10Kb may be called long fragments, and those shorter than 1Kb or 800bp may be called short fragments; it may include double-end sequencing, single-end sequencing and / or paired-end sequencing, etc., where the so-called double-end sequencing or paired-end sequencing may refer to the reading of any two segments or two parts of the same nucleic acid molecule that do not completely overlap.

[0057] Sequencing can be performed through a sequencing platform. According to the embodiments of the present application, the available sequencing platforms include, but are not limited to, Illumina's Hiseq, Miseq, Nextseq and Novasek sequencing platforms, Thermo Fisher / Life Technologies' Ion Torrent platform, BGISEQ and MGISEQ / DNBSEQ platforms of BGI, and single-molecule sequencing platforms; the sequencing method can be selected from single-end sequencing, double-end sequencing, or a sequencing method supported by the selected automated sequencing platform, etc.

[0058] Sequencing generally includes: library preparation, PCR amplification (optional), sequencing and data analysis. The sequence read out by sequencing is called sequencing sequence, also known as reads.

[0059] In some examples, sequencing by synthesis is performed for multiple rounds to obtain sequencing sequences or reads. For example, the sample to be tested is contacted with a polymerase and a modified nucleotide and placed under conditions suitable for a polymerization reaction, and the modified nucleotide is controllably incorporated into the sample to be tested or a single base extension is controllably achieved, and the corresponding reaction signal is detected, and the type of nucleotide incorporated into the sample to be tested in this reaction is determined based on the signal, and multiple controllable single base extensions and corresponding signal detections are performed in this way, so that the type of nucleotides or bases incorporated into the sample to be tested in multiple or multiple rounds of reactions can be detected according to the reaction signal information, so as to read out a portion of the sequence of the sample to be tested.

[0060] The so-called sample to be tested, also called the test template or template, can be a single molecule before amplification, or a molecular cluster or long chain containing multiple identical polynucleotide molecules after amplification, such as the clone cluster or DNA nanoball DNB formed by bridge amplification or rolling circle amplification used by mainstream sequencing platforms. The sample to be tested can be in the form of a single strand, double strand and / or a complex hybridized with a probe or primer.

[0061] The corresponding reaction signal can be, for example, a fluorescent signal, or it can be converted into image data formed by collecting these fluorescent signals. Thus, the image data are processed and analyzed to detect the nucleotides incorporated into the sample to be tested in each time or each round of reaction, so as to determine a part of the base sequence of the sample to be tested.

[0062] Specifically, in some examples, sequencing is achieved based on surface fluorescence imaging detection, and the sample to be tested is connected to a solid surface. For example, the nucleotides can be modified so that the nucleotides carry or can be combined with fluorescent labels, and carry removable inhibitory groups that can prevent other nucleotides from polymerizing and connecting to the next position of the sample to be tested (such modified nucleotides are also called reversible terminators), and after each polymerization reaction or single-base extension reaction is completed, the fluorescent label is stimulated to emit light, and these luminescent signals are collected to obtain an image of the sample to be tested in which a single-base extension reaction has occurred at a specified surface position; then, the inhibitory group and the fluorescent label are removed to perform the next polymerization reaction or the next round of polymerization reaction and signal collection (photography), and this polymerization-photography-excision is repeated multiple times or multiple rounds to obtain image set information related to the nucleotides connected to the sample to be tested in each single-base extension reaction.

[0063] It can be understood that the sample to be tested at the designated position on the surface undergoes a polymerization reaction and emits fluorescence, which generally appears as a bright spot or bright spot (spots) with a higher signal intensity than the background at the corresponding position of the image collected in this round of reaction. Therefore, based on the information of the bright spots corresponding to the specific chemical characteristics (the sample to be tested undergoing a polymerization reaction) included in the image sets, it can be determined whether the sample to be tested at the designated position undergoes a polymerization reaction, and combined with the preset correspondence between the distinguishable fluorescent luminescence signal and the type of nucleotide, the type of nucleotide that undergoes a polymerization reaction and is polymerized and connected to the sample to be tested can be detected, thereby determining at least a portion of the sequence of the sample to be tested to obtain the so-called read segment.

[0064] It should be noted that the so-called nucleotides include ribonucleic acid or deoxyribonucleic acid, including natural nucleotides or their derivatives or modifications (also called modified nucleotides or modified nucleotides, etc.). In this article, sometimes the base contained in the nucleotide is also used to refer to the nucleotide, which can be clearly known by those skilled in the art based on common knowledge and / or context.

[0065] See also Figure 1 The embodiment of the present application provides a method for controlling liquid inflow of a liquid circuit system 100, wherein the liquid circuit system 100 includes a flow cell 10 and a pump valve assembly 20, wherein the flow cell 10 includes a plurality of fluid channels 11, and each fluid channel 11 is provided with an inlet 12 and an outlet 13; the method includes:

[0066] A plurality of samples to be tested are sucked from the sample box 30 by using the pump valve assembly 20, and each sample to be tested is pumped into the fluid channel 11 from the outlet 13 of a corresponding fluid channel 11; or,

[0067] The pump-valve assembly 20 is used to draw a single sample to be tested from the sample box 30 , and the single sample to be tested is pumped into the fluid channel 11 from the inlet 12 of each fluid channel 11 .

[0068] An embodiment of the present application provides a liquid circuit system 100, which includes a flow pool 10 and a pump-valve assembly 20. The flow pool 10 includes a plurality of fluid channels 11, each of which is provided with an inlet 12 and an outlet 13. The pump-valve assembly 20 is used to draw a plurality of samples to be tested from a sample box 30, and pump each sample to be tested into the fluid channel 11 from the outlet 13 of a corresponding fluid channel 11; or, the pump-valve assembly 20 is used to draw a single sample to be tested from the sample box 30, and pump the single sample to be tested into the fluid channel 11 from the inlet 12 of each fluid channel 11.

[0069] The embodiment of the present application provides a computer device, including a processor and a memory, wherein the memory stores a computer program, and when the computer program is executed by the processor, the processor implements the method for controlling the flow of liquid in the liquid path system 100 of any of the above embodiments. For example, the processor is used to use the pump-valve assembly 20 to draw a plurality of samples to be tested from the sample box 30, and pump each sample to be tested into the fluid channel 11 from the outlet 13 of each corresponding fluid channel 11; or to use the pump-valve assembly 20 to draw a single sample to be tested from the sample box 30, and pump the single sample to be tested into the fluid channel 11 from the inlet 12 of each fluid channel 11.

[0070] In this way, the pump-valve assembly 20 can allow a single sample to be tested to enter the fluid channel 11 from the inlet 12 of each fluid channel 11, and can also allow each of the multiple samples to be tested to enter the fluid channel 11 from the outlet 13 of each corresponding fluid channel 11, so that different injection methods can be selected according to the number of samples to be tested, thereby improving the flexibility of loading the samples to be tested; in addition, by allowing each of the multiple samples to be tested to enter the fluid channel 11 from the outlet 13 of each corresponding fluid channel 11, cross-contamination between the multiple samples to be tested is avoided, thereby improving the sequencing quality and sequencing efficiency.

[0071] Specifically, the flow cell 10 is used to provide a place for sequencing and performing biochemical reactions. The flow cell 10 can also be called a chip. The flow cell 10 can be detachably connected to the pump valve assembly 20. The fluid channel 11 has a space for accommodating liquid, which can accommodate samples to be tested and reagents, so that the samples to be tested and the reagents undergo biochemical reactions, and the samples to be tested can be fixed on the inner surface of the fluid channel 11. The multiple fluid channels 11 in the flow cell 10 are arranged in parallel, and the number of fluid channels 11 can be three, four, five, six, etc. The size of each fluid channel 11 can be exactly the same or different in size. In some embodiments, the fluid channel 11 has a non-circular cross-section or an approximately rectangular cross-section, the fluid channel 11 has a width of at least 2 mm, about 4 mm, or about 7 mm, and the height of the fluid channel 11 can be at least 0.8 mm, about 1.2 mm.

[0072] The sample to be tested includes at least one nucleic acid molecule, and the reagent includes a polymerase and at least one nucleotide molecule, wherein the polymerase is used to bind the nucleotide molecule to the nucleic acid molecule. The base types between the multiple nucleotide molecules can be the same or different. The nucleotide molecule carries a blocking group, which is used to block the binding of more than one nucleotide on the nucleic acid molecule. The blocking group carries an optically detectable label, which allows the reaction between the nucleotide molecule and the nucleic acid molecule to be detected, so as to achieve the sequence determination of the nucleic acid molecule.

[0073] The pump-valve assembly 20 includes at least one pump group 22 and at least one valve, and the pump-valve assembly 20 is used to control the flow direction of the sample to be tested. The sample box 30 can be selectively connected to the inlet 12 of the fluid channel 11 or the outlet 13 of the fluid channel 11 through the pump-valve assembly 20. When the sample box 30 is connected to the inlet 12 of the fluid channel 11, the pump-valve assembly 20 pumps a single sample to be tested in the sample box 30 from the inlet 12 of each fluid channel 11 into the fluid channel 11; when the sample box 30 is connected to the outlet 13 of the fluid channel 11, the pump-valve assembly 20 pumps multiple samples to be tested in the sample box 30 from the outlet 13 of each corresponding fluid channel 11 into the fluid channel 11.

[0074] The sample box 30 is used to store samples to be tested. Furthermore, the sample box 30 can receive reagents or recovered samples.

[0075] See also Figure 1 In some embodiments, the fluid path system 100 includes a plurality of buffer regions 40, and the plurality of buffer regions 40 are arranged in one-to-one correspondence with the plurality of fluid channels 11;

[0076] Pumping each sample to be tested into the fluid channel 11 from the outlet 13 of each corresponding fluid channel 11, comprising:

[0077] Each sample to be tested is pumped to a corresponding buffer area 40 , and the sample to be tested in each buffer area 40 is pumped into the fluid channel 11 from the outlet 13 of the corresponding fluid channel 11 .

[0078] In some embodiments, the pump-valve assembly 20 is used to pump each sample to be tested to a corresponding buffer area 40 , and to pump the sample to be tested in each buffer area 40 into the fluid channel 11 from the outlet 13 of the corresponding fluid channel 11 .

[0079] In some embodiments, the processor is used to pump each sample to be tested to a corresponding buffer area 40 using the pump valve assembly 20 , and pump the sample to be tested in each buffer area 40 into the fluid channel 11 from the outlet 13 of the corresponding fluid channel 11 .

[0080] In this way, each cache area 40 can store one of the multiple samples to be tested, so that the pump-valve assembly 20 can pump each sample to be tested from the outlet 13 of the corresponding fluid channel 11 into the fluid channel 11 through the corresponding cache area 40, thereby realizing the reverse flow of the sample to be tested in the fluid channel 11, increasing the flexibility of loading the sample to be tested, and at the same time avoiding cross contamination between multiple samples to be tested, thereby improving sequencing quality and sequencing efficiency.

[0081] Specifically, the buffer area 40 can be a pipeline or a liquid storage bottle. The buffer area 40 is selectively connected to the sample box 30 and the outlet 13 of the fluid channel 11. When the buffer area 40 is connected to the sample box 30, the pump valve assembly 20 pumps each sample to be tested in the sample box 30 to the corresponding buffer area 40; when the buffer area 40 is connected to the outlet 13 of the fluid channel 11, the pump valve assembly 20 pumps the sample to be tested in each buffer area 40 from the outlet 13 of the corresponding fluid channel 11 into the fluid channel 11.

[0082] In certain embodiments, the method further comprises:

[0083] The pump-valve assembly 20 is used to draw reagents from the reagent kit 50 and pump the reagents to each buffer area 40 , and the reagents in each buffer area 40 are pumped into the fluid channel 11 from the outlet 13 of the corresponding fluid channel 11 .

[0084] In some embodiments, the pump-valve assembly 20 is used to draw reagents from the reagent kit 50 and pump the reagents to each buffer area 40 , and pump the reagents in each buffer area 40 into the fluid channel 11 from the outlet 13 of the corresponding fluid channel 11 .

[0085] In some embodiments, the processor is used to use the pump valve assembly 20 to draw reagents from the reagent kit 50 and pump the reagents to each cache area 40 , and pump the reagents in each cache area 40 into the fluid channel 11 from the outlet 13 of the corresponding fluid channel 11 .

[0086] In this way, the cache area 40 can store reagents, so that the pump-valve assembly 20 can pump the reagents into the fluid channel 11 from the outlet 13 of the corresponding fluid channel 11 through each cache area 40, thereby realizing the reverse flow of the reagents in the fluid channel 11, increasing the flexibility of reagent loading, while reducing the sequencing time and improving the sequencing efficiency.

[0087] Specifically, the reagent kit 50 is used to store reagents, including but not limited to DNA primers, DNA fragmentation enzyme, T4 DNA polymerase, Klenow enzyme, bases, ligase, DNA linker, T4 polynucleotide kinase, cleaning solution and / or purified water, etc. For example, the DNA primers in the reagent kit 50 are pumped into the fluid channel 11, so that the DNA primers can be connected to the flow cell 10 through a covalent bond.

[0088] The cache area 40 is selectively connected to the reagent box 50 and the outlet 13 of the fluid channel 11. When the cache area 40 is connected to the reagent box 50, the pump-valve assembly 20 pumps the reagent in the reagent box 50 to each cache area 40; when the cache area 40 is connected to the outlet 13 of the fluid channel 11, the pump-valve assembly 20 pumps the reagent in each cache area 40 from the outlet 13 of the corresponding fluid channel 11 into the fluid channel 11.

[0089] In certain embodiments, the pump-valve assembly 20 pumps the sample to be tested and / or the reagent to the buffer area 40 in a negative pressure driven manner.

[0090] In this way, the pump-valve assembly 20 can generate negative pressure to drive the sample to be tested and / or the reagent to move toward the buffer area 40, thereby realizing the transportation of the sample to be tested and / or the reagent.

[0091] Specifically, the pump-valve assembly 20 can pump the sample to be tested from the sample box 30 to the cache area 40 in a negative pressure-driven manner, and the pump-valve assembly 20 can also pump the reagent from the reagent box 50 to the cache area 40 in a negative pressure-driven manner. The pump-valve assembly 20 can also pump the sample to be tested and the reagent to the cache area 40 in a negative pressure-driven manner.

[0092] In certain embodiments, the pump-valve assembly 20 pumps the sample to be tested and / or reagent in the buffer area 40 into the fluid channel 11 from the outlet 13 of the fluid channel 11 in a positive pressure-driven manner.

[0093] In this way, the pump-valve assembly 20 can generate positive pressure to drive the sample to be tested and / or the reagent to move from the buffer area 40 to the fluid channel 11, thereby realizing the transportation of the sample to be tested and / or the reagent.

[0094] Specifically, the pump-valve assembly 20 can pump the sample to be tested in the cache area 40 into the fluid channel 11 from the outlet 13 of the fluid channel 11 in a positive pressure-driven manner, and can also pump the reagent in the cache area 40 into the fluid channel 11 from the outlet 13 of the fluid channel 11 in a positive pressure-driven manner, and can also pump the sample to be tested and the reagent in the cache area 40 into the fluid channel 11 from the outlet 13 of the fluid channel 11 in a positive pressure-driven manner.

[0095] See also Figure 2 In some embodiments, before using the pump valve assembly 20 to draw a plurality of samples to be tested from the sample box 30, the method includes:

[0096] S10 , using the pump valve assembly 20 to draw reagents from the reagent box 50 , and pumping the reagents into the sample box 30 to mix with each sample to be tested in the sample box 30 .

[0097] In some embodiments, the pump-valve assembly 20 is used to draw reagents from the reagent cartridge 50 and pump the reagents into the sample cartridge 30 to mix with each sample to be tested in the sample cartridge 30 .

[0098] In some embodiments, the processor is used to draw reagents from the reagent cartridge 50 using the pump-valve assembly 20 , and pump the reagents into the sample cartridge 30 to mix with each sample to be tested in the sample cartridge 30 .

[0099] In this way, each sample to be tested and the reagent can be mixed in the sample box 30 before entering the fluid channel 11 to process the sample to be tested, saving the time required for processing the sample to be tested before the machine, which is conducive to industrialization.

[0100] Specifically, the reagent and the sample to be tested can be mixed evenly by a rotation mixing method, a finger-snapping mixing method, an inversion mixing method, a pipette mixing method, a stirring mixing method, etc. For example, when preparing a library, DNA fragmentation enzyme, T4 DNA polymerase, Klenow enzyme, A base, ligase, and DNA adapter are mixed with the sample to be tested, the DNA fragmentation enzyme can fragment the sample to be tested, the T4 DNA polymerase can fill the two ends of the DNA fragment, the Klenow enzyme adds an A base to the 3' end of the DNA fragment, and the ligase connects the DNA adapter to the two ends of the DNA fragment.

[0101] See also Figure 2 In some embodiments, the pump valve assembly 20 is used to draw a plurality of samples to be tested from the sample box 30, including:

[0102] S20 , using the pump valve assembly 20 to draw a mixture of each sample to be tested and the reagent from the sample box 30 .

[0103] In some embodiments, the pump valve assembly 20 is used to draw a mixture of each sample to be tested and a reagent from the sample box 30 .

[0104] In some embodiments, the processor is used to use the pump valve assembly 20 to absorb the mixed solution of each sample to be tested and the reagent from the sample box 30.

[0105] In this way, the sample box 30 can store the mixture of each sample to be tested and the reagent, making it convenient for the pump valve assembly 20 to absorb the sample to be tested and / or the mixture of the sample to be tested and the reagent from the sample box 30, thereby improving the utilization rate of the sample box 30, reducing the number of containers for storing the mixture of the sample to be tested and the reagent, and reducing the structure of the liquid path system 100, thereby saving space.

[0106] Specifically, the sample box 30 can store each sample to be tested, or mix each sample to be tested with a reagent and store the mixed solution. The pump valve assembly 20 can absorb each sample to be tested from the sample box 30, or absorb the mixed solution of each sample to be tested and the reagent.

[0107] See also Figure 2 In some embodiments, each sample to be tested is pumped into the fluid channel 11 from the outlet 13 of each corresponding fluid channel 11, comprising:

[0108] S30 , using the pump-valve assembly 20 to pump the mixture of each sample to be tested and the reagent into the fluid channel 11 from the outlet 13 of a corresponding fluid channel 11 .

[0109] In some embodiments, the pump-valve assembly 20 is used to pump the mixture of each sample to be tested and the reagent into the fluid channel 11 from the outlet 13 of a corresponding fluid channel 11 .

[0110] In some embodiments, the processor is configured to use the pump-valve assembly 20 to pump the mixture of each sample to be tested and the reagent into the fluid channel 11 from the outlet 13 of a corresponding fluid channel 11 .

[0111] In this way, the pump-valve assembly 20 can allow each mixture of the sample to be tested and the reagent to enter the fluid channel 11 from the outlet 13 of a corresponding fluid channel 11. The reverse flow of the mixture of the sample to be tested and the reagent in the fluid channel 11 increases the flexibility of loading the sample to be tested, while reducing the sequencing time and improving the sequencing efficiency.

[0112] Specifically, the pump valve assembly 20 can pump the mixture of the sample to be tested and the reagent into the fluid channel 11 from the outlet 13 of the fluid channel 11 , and make the mixture of the sample to be tested and the reagent flow out of the fluid channel 11 from the inlet 12 of the fluid channel 11 .

[0113] See also Figure 1 In some embodiments, the fluid path system 100 includes a plurality of buffer regions 40, and the plurality of buffer regions 40 are arranged in one-to-one correspondence with the plurality of fluid channels 11;

[0114] The pump valve assembly 20 is used to draw reagents from the reagent box 50, and the reagents are pumped into the sample box 30 to mix with each sample to be tested in the sample box 30, including:

[0115] The pump valve assembly 20 is used to pump the reagent to each buffer area 40 , and the reagent in each buffer area 40 is pumped into the sample box 30 to be mixed with the corresponding sample to be tested in the sample box 30 .

[0116] In some embodiments, the pump-valve assembly 20 is used to pump the reagent to each buffer area 40 , and to pump the reagent in each buffer area 40 into the sample box 30 to mix with the corresponding sample to be tested in the sample box 30 .

[0117] In some embodiments, the processor is used to pump the reagent to each buffer area 40 using the pump valve assembly 20 , and pump the reagent in each buffer area 40 into the sample box 30 to mix with the corresponding sample to be tested in the sample box 30 .

[0118] In this way, the buffer area 40 can store the reagent, so that the pump-valve assembly 20 can pump the reagent from the reagent cartridge 50 into the sample cartridge 30 through each buffer area 40 .

[0119] Specifically, the cache area 40 is selectively connected to the reagent box 50 and the sample box 30. When the cache area 40 is connected to the reagent box 50, the pump-valve assembly 20 pumps the reagent in the reagent box 50 to each cache area 40; when the cache area 40 is connected to the sample box 30, the pump-valve assembly 20 pumps the reagent in each cache area 40 into the sample box 30 to mix with the corresponding sample to be tested in the sample box 30.

[0120] In some embodiments, the mixture of each sample to be tested and the reagent is pumped into the fluid channel 11 from the outlet 13 of a corresponding fluid channel 11, comprising:

[0121] The mixed solution of each sample to be tested and the reagent is pumped back to the corresponding buffer area 40 , and the mixed solution of each sample to be tested and the reagent in each buffer area 40 is pumped into the fluid channel 11 from the outlet 13 of the corresponding fluid channel 11 .

[0122] In some embodiments, the pump valve assembly 20 is used to pump the mixture of each sample to be tested and the reagent back to the corresponding cache area 40, and to pump the mixture of the sample to be tested and the reagent in each cache area 40 into the fluid channel 11 from the outlet 13 of the corresponding fluid channel 11.

[0123] In some embodiments, the processor is used to pump the mixture of each test sample and reagent back to the corresponding cache area 40, and pump the mixture of the test sample and reagent in each cache area 40 into the fluid channel 11 from the outlet 13 of the corresponding fluid channel 11.

[0124] In this way, the cache area 40 can store each mixture of the sample to be tested and the reagent separately, so that the pump-valve assembly 20 can pump each mixture of the sample to be tested and the reagent into the fluid channel 11 from the outlet 13 of the corresponding fluid channel 11 through the respective corresponding cache area 40, thereby realizing the reverse flow of the mixture of the sample to be tested and the reagent in the fluid channel 11, increasing the flexibility of loading the sample to be tested, and at the same time avoiding cross contamination between the mixtures of multiple samples to be tested and reagents, thereby improving the sequencing quality and sequencing efficiency.

[0125] Specifically, the cache area 40 is selectively connected to the sample box 30 and the outlet 13 of the fluid channel 11. When the cache area 40 is connected to the sample box 30, the pump-valve assembly 20 pumps each mixed liquid in the sample box 30 to each cache area 40; when the cache area 40 is connected to the outlet 13 of the fluid channel 11, the pump-valve assembly 20 pumps the mixed liquid in each cache area 40 from the outlet 13 of the corresponding fluid channel 11 into the fluid channel 11.

[0126] In some embodiments, the pump-valve assembly 20 pumps the mixture of the sample to be tested and the reagent to the buffer area 40 in a negative pressure driven manner.

[0127] In this way, the pump-valve assembly 20 can generate negative pressure to drive the mixed solution of the sample to be tested and the reagent to move toward the buffer area 40 to achieve the transportation of the mixed solution of the sample to be tested and the reagent.

[0128] In some embodiments, the pump-valve assembly 20 pumps the mixed solution of the sample to be tested and the reagent in the buffer area 40 into the fluid channel 11 from the outlet 13 of the fluid channel 11 in a positive pressure-driven manner.

[0129] In this way, the pump valve assembly 20 can generate positive pressure to drive the mixed solution of the sample to be tested and the reagent to move from the buffer area 40 to the fluid channel 11, thereby realizing the transportation of the mixed solution of the sample to be tested and the reagent.

[0130] Specifically, the pump-valve assembly 20 can pump the reagent from the test kit 50 to the cache area 40 in a negative pressure-driven manner, and then pump the reagent in the cache area 40 into the sample box 30 in a positive pressure-driven manner to mix with the sample to be tested in the sample box 30, and then pump the mixture of the sample to be tested and the reagent from the sample box 30 to the cache area 40 in a negative pressure-driven manner, and finally pump the mixture of the sample to be tested and the reagent in the cache area 40 from the outlet 13 of the fluid channel 11 into the fluid channel 11 in a positive pressure-driven manner.

[0131] In certain embodiments, the method further comprises:

[0132] The reagent is sucked from the reagent cartridge 50 by the pump-valve assembly 20 , and the reagent is pumped into the fluid channel 11 from the inlet 12 of each fluid channel 11 .

[0133] In certain embodiments, the pump-valve assembly 20 is used to draw reagents from the reagent cartridge 50 and pump the reagents into the fluid channels 11 from the inlet 12 of each fluid channel 11 .

[0134] In some embodiments, the processor is configured to draw reagents from the reagent cartridge 50 using the pump-valve assembly 20 and pump the reagents into the fluid channels 11 from the inlet 12 of each fluid channel 11 .

[0135] In this way, the pump-valve assembly 20 can allow the reagent to enter the fluid channel 11 from the outlet 13 of each fluid channel 11, and can also allow the reagent to enter the fluid channel 11 from the inlet 12 of the fluid channel 11. The bidirectional flow of the reagent in the fluid channel 11 increases the flexibility of reagent loading, while reducing the sequencing time and improving the sequencing efficiency.

[0136] Specifically, the test kit 50 can be selectively connected to the inlet 12 of the fluid channel 11 or the outlet 13 of the fluid channel 11 through the pump-valve assembly 20. When the test kit 50 is connected to the inlet 12 of the fluid channel 11, the pump-valve assembly 20 pumps the reagent in the test kit 50 from the inlet 12 of each fluid channel 11 into the fluid channel 11; when the test kit 50 is connected to the outlet 13 of the fluid channel 11, the pump-valve assembly 20 pumps the reagent in the test kit 50 from the outlet 13 of each fluid channel 11 into the fluid channel 11.

[0137] In certain embodiments, the pump-valve assembly 20 pumps a single sample to be tested and / or a reagent from the inlet 12 of each fluid channel 11 into the fluid channel 11 in a negative pressure driven manner.

[0138] In this way, the pump-valve assembly 20 can generate negative pressure to drive a single sample to be tested and / or a reagent to move toward each fluid channel 11, thereby achieving the transportation of a single sample to be tested and / or a reagent.

[0139] Specifically, the pump-valve assembly 20 can pump a single sample to be tested in the sample box 30 into each fluid channel 11 from the inlet 12 of the fluid channel 11 in a negative pressure-driven manner, and can also pump the reagent in the reagent kit 50 into each fluid channel 11 from the inlet 12 of the fluid channel 11 in a negative pressure-driven manner, and can also pump a single sample to be tested and the reagent into each fluid channel 11 from the inlet 12 of the fluid channel 11 in a negative pressure-driven manner.

[0140] See also Figure 1 , in certain embodiments, the pump valve assembly 20 includes a first flow path selection valve 21 and a pump group 22;

[0141] The first flow path selection valve 21 and the pump group 22 are both located downstream of the flow pool 10;

[0142] The first flow path selection valve 21 is used to connect the pump group 22 with the fluid channel 11, and / or connect the pump group 22 with the sample box 30;

[0143] A plurality of samples to be tested are sucked from the sample box 30 through the first flow path selection valve 21 and the pump group 22 , and each sample to be tested enters the fluid channel 11 from the outlet 13 of a corresponding fluid channel 11 .

[0144] In some embodiments, the first flow path selection valve 21 and the pump group 22 are used to draw a plurality of samples to be tested from the sample box 30 and allow each sample to be tested to enter the fluid channel 11 from the outlet 13 of a corresponding fluid channel 11 .

[0145] In some embodiments, the processor is used to draw multiple samples to be tested from the sample box 30 through the first flow path selection valve 21 and the pump group 22, and make each sample to be tested enter the fluid channel 11 from the outlet 13 of each corresponding fluid channel 11.

[0146] In this way, the sample box 30 and each fluid channel 11 can be connected through the first flow path selection valve 21 to achieve control of multiple flow paths of samples to be tested.

[0147] Specifically, the first flow path selection valve 21 and the pump group 22 are connected to the outlet 13 of each fluid channel 11. Figure 1As shown by the dotted line, the first flow path selection valve 21 can connect the pump group 22 with the sample box 30, so that the pump group 22 draws multiple samples to be tested from the sample box 30. The first flow path selection valve 21 can also connect the pump group 22 with the outlet 13 of each fluid channel 11, so that the pump group 22 drives each sample to be tested to enter the fluid channel 11 from the outlet 13 of each corresponding fluid channel 11. The pump group 22 can be a peristaltic pump, a plunger pump, a syringe pump, a gear pump or a diaphragm pump, etc. The pump group 22 can provide negative pressure or positive pressure. The pump group 22 includes multiple single pumps, and the multiple single pumps correspond to the multiple fluid channels 11 one by one.

[0148] See also Figure 1 In some embodiments, a plurality of buffer areas 40 are provided between the first flow path selection valve 21 and the pump group 22, and the plurality of buffer areas 40 are provided in one-to-one correspondence with the plurality of fluid channels 11;

[0149] A plurality of samples to be tested are sucked from the sample box 30 through the first flow path selection valve 21 and the pump group 22, and each sample to be tested enters the fluid channel 11 from the outlet 13 of each corresponding fluid channel 11, including:

[0150] Each sample to be tested is pumped from the sample box 30 to a corresponding cache area 40 through the first flow path selection valve 21 by the pump group 22, and the sample to be tested in each cache area 40 is pumped into the fluid channel 11 from the outlet 13 of the corresponding fluid channel 11 through the first flow path selection valve 21.

[0151] In some embodiments, the pump group 22 is used to pump each sample to be tested from the sample box 30 to a corresponding cache area 40 through the first flow path selection valve 21, and to pump the sample to be tested in each cache area 40 from the outlet 13 of the corresponding fluid channel 11 into the fluid channel 11 through the first flow path selection valve 21.

[0152] In some embodiments, the processor is used to pump each sample to be tested from the sample box 30 through the first flow path selection valve 21 to a corresponding cache area 40 via the pump group 22, and to pump the sample to be tested in each cache area 40 from the outlet 13 of the corresponding fluid channel 11 into the fluid channel 11 via the first flow path selection valve 21.

[0153] In this way, the first flow path selection valve 21 can select the sample box 30 and the fluid channel 11 to communicate with the buffer area 40, thereby realizing the control of switching the flow paths of multiple samples to be tested.

[0154] Specifically, the first flow path selection valve 21 can connect each cache area 40 and the sample box 30, so that the pump group 22 pumps each of the multiple samples to be tested from the sample box 30 to a corresponding cache area 40 through the first flow path selection valve 21. The first flow path selection valve 21 can also connect each cache area 40 and the outlet 13 of the corresponding fluid channel 11, so that the pump group 22 pumps the samples to be tested in each cache area 40 from the outlet 13 of the corresponding fluid channel 11 into the fluid channel 11 through the first flow path selection valve 21.

[0155] See also Figure 1 , in certain embodiments, the pump valve assembly 20 further includes a second flow path selection valve 23;

[0156] The second flow path selection valve 23 is located upstream of the flow pool 10 , and the second flow path selection valve 23 is used to connect the reagent kit 50 with the first flow path selection valve 21 ;

[0157] The reagent is sucked from the reagent cartridge 50 through the first flow path selection valve 21 , the second flow path selection valve 23 and the pump unit 22 , and is allowed to enter the fluid channel 11 from the outlet 13 of the fluid channel 11 .

[0158] In certain embodiments, the first flow path selection valve 21 , the second flow path selection valve 23 and the pump group 22 are used to draw reagents from the reagent cartridge 50 and allow the reagents to enter the fluid channel 11 from the outlet 13 of the fluid channel 11 .

[0159] In some embodiments, the processor is used to draw reagents from the reagent cartridge 50 through the first flow path selection valve 21 , the second flow path selection valve 23 and the pump unit 22 , and allow the reagents to enter the fluid channel 11 from the outlet 13 of the fluid channel 11 .

[0160] In this way, the reagent chamber 50 and the fluid channel 11 can be connected through the first flow path selection valve 21 and the second flow path selection valve 23 to achieve control of the reagent flow path.

[0161] Specifically, the second flow path selection valve 23 is connected to the reagent box 50. Figure 1 As shown by the dotted line, the first flow path selection valve 21 can connect the pump group 22 and the second flow path selection valve 23, so that the pump group 22 draws the reagent from the reagent box 50. The first flow path selection valve 21 can also connect the pump group 22 and the outlet 13 of the fluid channel 11, so that the pump group 22 drives the reagent to enter the fluid channel 11 from the outlet 13 of the fluid channel 11. The second flow path selection valve 23 can be a reversing device such as a rotary valve, a pneumatic reversing valve, an electro-hydraulic reversing valve, a manual reversing valve, a piezoelectric valve, a pinch valve or a rotary cutting valve.

[0162] In some embodiments, the reagent is drawn from the reagent box 50 through the first flow path selection valve 21, the second flow path selection valve 23 and the pump group 22, and the reagent enters the fluid channel 11 from the outlet 13 of the fluid channel 11, including:

[0163] The reagent is pumped from the reagent box 50 to each cache area 40 through the second flow path selection valve 23 and the first flow path selection valve 21 by the pump group 22, and the reagent in each cache area 40 is pumped into the fluid channel 11 from the outlet 13 of the corresponding fluid channel 11 through the first flow path selection valve 21.

[0164] In certain embodiments, the pump group 22 is used to pump the reagent from the reagent box 50 to each cache area 40 through the second flow path selection valve 23 and the first flow path selection valve 21, and to pump the reagent in each cache area 40 from the outlet 13 of the corresponding fluid channel 11 into the fluid channel 11 through the first flow path selection valve 21.

[0165] In certain embodiments, the processor is used to pump the reagent from the reagent box 50 through the second flow path selection valve 23 and the first flow path selection valve 21 to each cache area 40 via the pump group 22, and to pump the reagent of each cache area 40 from the outlet 13 of the corresponding fluid channel 11 into the fluid channel 11 through the first flow path selection valve 21.

[0166] In this way, the first flow path selection valve 21 can select the second flow path selection valve 23 and the fluid channel 11 to communicate with the buffer area 40 , thereby controlling the flow path switching of the reagent in the reagent kit 50 connected to the second flow path selection valve 23 .

[0167] Specifically, the second flow path selection valve 23 connects the reagent kit 50 and the first flow path selection valve 21, and the first flow path selection valve 21 can connect each cache area 40 and the second flow path selection valve 23, so that the pump group 22 pumps the reagent from the reagent kit 50 to each cache area 40 through the second flow path selection valve 23 and the first flow path selection valve 21. The first flow path selection valve 21 can also connect each cache area 40 and the outlet 13 of the corresponding fluid channel 11, so that the pump group 22 pumps the reagent in each cache area 40 from the outlet 13 of the corresponding fluid channel 11 into the fluid channel 11 through the first flow path selection valve 21.

[0168] In some embodiments, the pump group 22 pumps the sample to be tested and / or the reagent to the buffer area 40 through the first flow path selection valve 21 in a negative pressure driven manner.

[0169] In this way, the pump group 22 can generate negative pressure to drive the sample to be tested and / or the reagent to move to the buffer area 40, thereby realizing the transportation of the sample to be tested and / or the reagent.

[0170] Specifically, the pump group 22 can pump the sample to be tested from the sample box 30 through the first flow path selection valve 21 to the cache area 40 in a negative pressure-driven manner. The pump group 22 can also pump the reagent from the reagent box 50 through the second flow path selection valve 23 and the first flow path selection valve 21 to the cache area 40 in a negative pressure-driven manner. The pump group 22 can also pump the sample to be tested and the reagent through the first flow path selection valve 21 to the cache area 40 in a negative pressure-driven manner.

[0171] In some embodiments, the pump group 22 pumps the sample to be tested and / or reagent in the buffer area 40 into the fluid channel 11 from the outlet 13 of the fluid channel 11 through the first flow path selection valve 21 in a positive pressure driven manner.

[0172] In this way, the pump group 22 can generate positive pressure to drive the sample to be tested and / or the reagent to move from the buffer area 40 to the fluid channel 11 through the first flow path selection valve 21 to achieve the transportation of the sample to be tested and / or the reagent.

[0173] Specifically, the pump group 22 can pump the samples to be tested in the cache area 40 into the fluid channel 11 from the outlet 13 of the fluid channel 11 through the first flow path selection valve 21 in a positive pressure-driven manner, and can also pump the reagents in the cache area 40 into the fluid channel 11 from the outlet 13 of the fluid channel 11 through the first flow path selection valve 21 in a positive pressure-driven manner, and can also pump the samples to be tested and the reagents in the cache area 40 into the fluid channel 11 from the outlet 13 of the fluid channel 11 through the first flow path selection valve 21 in a positive pressure-driven manner.

[0174] See also Figure 1 , in certain embodiments, the pump valve assembly 20 further includes a second flow path selection valve 23;

[0175] The second flow path selection valve 23 is located upstream of the flow pool 10 , and the second flow path selection valve 23 is used to connect the reagent kit 50 with the first flow path selection valve 21 ;

[0176] Before aspirating a plurality of samples to be tested from the sample box 30 through the first flow path selection valve 21 and the pump group 22, the method further includes:

[0177] The reagent is drawn from the reagent box 50 through the first flow path selection valve 21 , the second flow path selection valve 23 and the pump group 22 , and is pumped into the sample box 30 to be mixed with the sample to be tested in the sample box 30 .

[0178] In some embodiments, the first flow path selection valve 21 , the second flow path selection valve 23 and the pump group 22 are used to draw reagents from the reagent box 50 and pump the reagents into the sample box 30 to mix with the sample to be tested in the sample box 30 .

[0179] In some embodiments, the processor is used to draw reagents from the reagent box 50 through the first flow path selection valve 21 , the second flow path selection valve 23 and the pump group 22 , and pump the reagents into the sample box 30 to mix with the sample to be tested in the sample box 30 .

[0180] In this way, the reagent box 50 and the sample box 30 can be connected through the first flow path selection valve 21 and the second flow path selection valve 23 to achieve control of the reagent flow path.

[0181] Specifically, Figure 1 As shown by the dotted line, the second flow path selection valve 23 is connected to the reagent box 50, and the first flow path selection valve 21 can connect the pump group 22 and the second flow path selection valve 23, so that the pump group 22 draws the reagent from the reagent box 50. The first flow path selection valve 21 can also connect the pump group 22 and the sample box 30, so that the pump group 22 drives the reagent into the sample box 30 to mix with the sample to be tested in the sample box 30.

[0182] See also Figure 3 In some embodiments, a plurality of buffer areas 40 are provided between the first flow path selection valve 21 and the pump group 22, and the plurality of buffer areas 40 are provided in one-to-one correspondence with the plurality of fluid channels 11;

[0183] The reagent is drawn from the reagent box 50 through the first flow path selection valve 21, the second flow path selection valve 23 and the pump group 22, and the reagent is pumped into the sample box 30 to mix with the sample to be tested in the sample box 30, including:

[0184] S11, the reagent is pumped from the test kit 50 into each cache area 40 through the first flow path selection valve 21 and the second flow path selection valve 23 by the pump group 22, and the reagent in each cache area 40 is pumped into the sample box 30 through the first flow path selection valve 21 to mix with the corresponding sample to be tested in the sample box 30.

[0185] In some embodiments, the pump group 22 is used to pump the reagent from the test kit 50 into each cache area 40 through the first flow path selection valve 21 and the second flow path selection valve 23, and pump the reagent in each cache area 40 into the sample box 30 through the first flow path selection valve 21 to mix with the corresponding sample to be tested in the sample box 30.

[0186] In some embodiments, the processor is used to pump the reagent from the test kit 50 into each cache area 40 through the first flow path selection valve 21 and the second flow path selection valve 23 via the pump group 22, and pump the reagent in each cache area 40 into the sample box 30 through the first flow path selection valve 21 to mix with the corresponding sample to be tested in the sample box 30.

[0187] In this way, the first flow path selection valve 21 can select the reagent box 50 and the sample box 30 to communicate with each buffer area 40, thereby realizing the control of reagent flow path switching.

[0188] Specifically, the second flow path selection valve 23 connects the reagent box 50 with the first flow path selection valve 21, and the first flow path selection valve 21 can connect each cache area 40 with the second flow path selection valve 23, so that the pump group 22 pumps the reagent from the reagent box 50 into each cache area 40 through the second flow path selection valve 23 and the first flow path selection valve 21. The first flow path selection valve 21 can also connect the pump group 22 with the sample box 30, so that the pump group 22 pumps the reagent in each cache area 40 into the sample box 30 through the first flow path selection valve 21 to mix with the corresponding sample to be tested in the sample box 30.

[0189] See also Figure 3 In some embodiments, a plurality of samples to be tested are sucked from the sample box 30 through the first flow path selection valve 21 and the pump group 22, and each sample to be tested enters the fluid channel 11 from the outlet 13 of the corresponding fluid channel 11, including:

[0190] S21, the mixed solution of each sample to be tested and the reagent is pumped to the corresponding cache area 40 through the first flow path selection valve 21 by the pump group 22, and the mixed solution of the sample to be tested and the reagent in each cache area 40 is pumped into the fluid channel 11 from the outlet 13 of the corresponding fluid channel 11 through the first flow path selection valve 21.

[0191] In some embodiments, the pump group 22 is used to pump the mixture of each sample to be tested and the reagent to each corresponding cache area 40 through the first flow path selection valve 21, and to pump the mixture of the sample to be tested and the reagent in each cache area 40 into the fluid channel 11 from the outlet 13 of the corresponding fluid channel 11 through the first flow path selection valve 21.

[0192] In some embodiments, the processor is used to pump each mixture of the sample to be tested and the reagent through the first flow path selection valve 21 to the corresponding cache area 40 through the pump group 22, and pump the mixture of the sample to be tested and the reagent in each cache area 40 into the fluid channel 11 from the outlet 13 of the corresponding fluid channel 11 through the first flow path selection valve 21.

[0193] In this way, the first flow path selection valve 21 can select the sample box 30 and the fluid channel 11 to communicate with each buffer area 40, so as to control the flow path switching of each mixed solution of the sample to be tested and the reagent.

[0194] Specifically, the first flow path selection valve 21 can connect each cache area 40 and the sample box 30, so that the pump group 22 pumps the mixture of each sample to be tested and the reagent from the sample box 30 to each cache area 40 through the first flow path selection valve 21. The first flow path selection valve 21 can also connect each cache area 40 and the outlet 13 of the corresponding fluid channel 11, so that the pump group 22 pumps the mixture of the sample to be tested and the reagent in each cache area 40 from the outlet 13 of the corresponding fluid channel 11 into the fluid channel 11 through the first flow path selection valve 21.

[0195] In some embodiments, the pump group 22 pumps the mixture of each sample to be tested and the reagent to a corresponding buffer area 40 through the first flow path selection valve 21 in a negative pressure driven manner.

[0196] In this way, the pump group 22 can generate negative pressure to drive the mixed solution of each sample to be tested and the reagent to move to the corresponding buffer area 40, thereby realizing the transportation of the mixed solution of multiple samples to be tested and the reagent.

[0197] In some embodiments, the pump group 22 pumps the mixture of the sample to be tested and the reagent in each buffer area 40 into the fluid channel 11 from the outlet 13 of the corresponding fluid channel 11 through the first flow path selection valve 21 in a positive pressure driven manner.

[0198] In this way, the pump group 22 can generate positive pressure to drive each mixed solution of the sample to be tested and the reagent to move from each buffer area 40 to the corresponding fluid channel 11, thereby realizing the transportation of multiple mixed solutions of the sample to be tested and the reagent.

[0199] Specifically, the pump group 22 can pump the reagent from the test kit 50 through the second flow path selection valve 23 and the first flow path selection valve 21 to each cache area 40 in a negative pressure-driven manner, and then pump the reagent in each cache area 40 through the first flow path selection valve 21 to the sample box 30 in a positive pressure-driven manner to mix with the corresponding sample to be tested in the sample box 30, and then pump the mixed solution of each sample to be tested and the reagent from the sample box 30 through the first flow path selection valve 21 to each cache area 40 in a negative pressure-driven manner, and finally pump the mixed solution of the sample to be tested and the reagent in each cache area 40 through the first flow path selection valve 21 from the outlet 13 of the corresponding fluid channel 11 into the fluid channel 11 in a positive pressure-driven manner.

[0200] See also Figure 1 , in certain embodiments, the pump valve assembly 20 further includes a second flow path selection valve 23 and a manifold assembly 24;

[0201] The second flow path selection valve 23 and the manifold assembly 24 are located upstream of the flow pool 10. The second flow path selection valve 23 is used to connect the reagent kit 50 with the manifold assembly 24, and / or connect the reagent kit 50 with the first flow path selection valve 21. The manifold assembly 24 is connected to the fluid channel 11 of the flow pool 10.

[0202] A single sample and / or reagent is drawn from the sample box 30 and / or reagent cartridge 50 through the second flow path selection valve 23 , the manifold assembly 24 and the pump group 22 , and enters the fluid channel 11 from the inlet 12 of each fluid channel 11 .

[0203] In some embodiments, the second flow path selection valve 23, the manifold assembly 24 and the pump group 22 are used to draw a single sample and / or reagent to be tested from the sample box 30 and / or the reagent box 50, and allow the single sample and / or reagent to be tested to enter the fluid channel 11 from the inlet 12 of each fluid channel 11.

[0204] In certain embodiments, the processor is used to draw a single sample and / or reagent to be tested from the sample box 30 and / or the reagent box 50 through the second flow path selection valve 23, the manifold assembly 24 and the pump group 22, and allow the single sample and / or reagent to be tested to enter the fluid channel 11 from the inlet 12 of each fluid channel 11.

[0205] In this way, the second flow path selection valve 23 can select the manifold assembly 24 and the first flow path selection valve 21 to communicate with the reagent box 50, so as to realize the control of the reagent flow path switching. In addition, the manifold assembly 24 can split a single sample to be tested and / or reagent, so that the samples to be tested entering different fluid channels 11 through the inlet 12 of each fluid channel 11 are the same sample to be tested or the same reagent.

[0206] Specifically, Figure 1 As shown by the double-dotted line, the manifold assembly 24 is connected to the inlet 12 of the fluid channel 11, and the second flow path selection valve 23 can connect the reagent kit 50 and the manifold assembly 24, so that the pump group 22 can pass the reagent of the reagent kit 50 through the second flow path selection valve 23 and the manifold assembly 24 from the inlet 12 of each fluid channel 11 into the fluid channel 11; the second flow path selection valve 23 can connect the sample box 30 and the manifold assembly 24, so that the pump group 22 can pass the sample to be tested in the sample box 30 through the second flow path selection valve 23 and the manifold assembly 24 from the inlet 12 of each fluid channel 11 into the fluid channel 11.

[0207] In one embodiment, the liquid path system 100 includes a waste liquid bottle 60, which is connected to the manifold assembly 24 and the pump group 22, and is used to store or discharge all or part of the waste liquid of the liquid path system 100. The waste liquid bottle 60 can be connected to the inlet 12 of each fluid channel 11 through the manifold assembly 24, so that the waste liquid after the reaction in the fluid channel 11 can flow into the waste liquid bottle 60 from the inlet 12 of each fluid channel 11. The waste liquid bottle 60 can also be connected to the outlet 13 of each fluid channel 11 through the pump group 22, so that the waste liquid after the reaction in the fluid channel 11 can flow into the waste liquid bottle 60 from the outlet 13 of each fluid channel 11. An electromagnetic valve 70 can be set between the waste liquid bottle 60 and the manifold assembly 24. When the electromagnetic valve 70 is energized, the pump group 22 can drive the waste liquid in each fluid channel 11 to flow from the inlet 12 of each fluid channel 11 into the waste liquid bottle 60 through the manifold assembly 24; when the electromagnetic valve 70 is de-energized, the pump group 22 can drive the waste liquid in each fluid channel 11 to flow from the outlet 13 of each fluid channel 11 into the waste liquid bottle 60 through the pump group 22.

[0208] In some embodiments, the pump group 22 pumps a single sample to be tested and / or a reagent into the fluid channel 11 from the inlet 12 of each fluid channel 11 in a negative pressure driven manner.

[0209] In this way, the pump group 22 can generate negative pressure to drive a single sample to be tested and / or a reagent to move toward each fluid channel 11, thereby realizing the transportation of a single sample to be tested and / or a reagent.

[0210] Specifically, the pump group 22 can pump a single sample to be tested from the sample box 30 into each fluid channel 11 through the second flow path selection valve 23 and the manifold assembly 24 in a negative pressure-driven manner. The pump group 22 can also pump the reagent from the reagent box 50 into each fluid channel 11 through the second flow path selection valve 23 and the manifold assembly 24 in a negative pressure-driven manner. The pump group 22 can also pump a single sample to be tested and the reagent into each fluid channel 11 through the second flow path selection valve 23 and the manifold assembly 24 in a negative pressure-driven manner.

[0211] See also Figure 1 and Figure 4 In some embodiments, the first flow path selection valve 21 includes multiple groups of ports, each group of ports is used to control the liquid inlet of a fluid channel 11, each group of ports includes a first port 211, a second port 212, a third port 213 and a fourth port 214, the first port 211 is connected to the outlet 13 of the fluid channel 11, the second port 212 is connected to the cache area 40, the third port 213 is connected to the sample box 30, and the fourth port 214 is connected to the reagent box 50, and any one of the first port 211, the third port 213, and the fourth port 214 is selectively connected to the second port 212.

[0212] In this way, the buffer area 40 can be selectively connected to the outlet 13 of the fluid channel 11, the sample box 30 and the reagent box 50 through the first flow path selection valve 21, so as to realize the control of the flow path switching of the sample to be tested and / or the reagent. Multiple groups of ports can control the liquid inlet of multiple fluid channels 11 at the same time, reducing the number of electromagnetic valves 70 used, thereby reducing the cost and occupied space of the liquid path system 100.

[0213] Specifically, the first port 211, the second port 212 and the third port 213 can be used as the liquid outlet of the first flow path selection valve 21, or as the liquid inlet, and the fourth port 214 can be used as the liquid inlet of the first flow path selection valve 21. The number of ports can be two groups, three groups, four groups, five groups, six groups or more, and the number of groups of ports can be consistent with the number of fluid channels 11, and the spacing between multiple groups of ports can be consistent. In one embodiment, the number of fluid channels 11 is four, and the first flow path selection valve 21 includes four groups of ports. The four first ports 211 are respectively connected to the outlets 13 of the four fluid channels 11, and the flow paths of the fluids in the four fluid channels 11 can be controlled simultaneously by the first flow path selection valve 21. The port can be a regular shape such as a circle, a polygon, or an irregular shape, and the shape of each port can be the same or different. In the embodiment of the present application, in order to facilitate the formation, manufacture and / or connection of the port with a common pipeline, the port is circular.

[0214] See also Figure 1 In some embodiments, the fluid system 100 includes a plurality of first pipelines 81, and the plurality of first pipelines 81 are arranged in one-to-one correspondence with the plurality of first ports 211, and the two ends of each first pipeline 81 are respectively connected to an outlet of a fluid channel 11 and a first port 211.

[0215] In this way, the samples and / or reagents to be tested in each buffer area 40 can flow from the second port 212 and the first port 211 into the corresponding fluid channel 11 via the first pipeline 81 to achieve the transportation of the samples and / or reagents to be tested.

[0216] Specifically, the first pipeline 81 may be a pipeline for transmitting fluid, the fluid including but not limited to gas, liquid, etc. The cross-sectional shape of the first pipeline 81 may be circular, and the length and diameter of the first pipeline 81 may be set according to actual needs. The number of first pipelines 81 may be consistent with the number of fluid channels 11, and the plurality of first pipelines 81 are respectively connected to the plurality of fluid channels 11 and the plurality of first ports 211 in a one-to-one correspondence.

[0217] See also Figure 1In some embodiments, the liquid circuit system 100 includes a plurality of second pipelines 82, and the plurality of second pipelines 82 are arranged in one-to-one correspondence with the plurality of third ports 213. The two ends of each second pipeline 82 are respectively connected to the sample box 30 and a third port 213. Each second pipeline 82 is used to allow a sample to be tested in the sample box 30 to pass through the first flow path selection valve 21 and enter the corresponding cache area 40.

[0218] In this way, the samples to be tested in the sample box 30 can flow from the multiple first ports 211 into the multiple buffer areas 40 through the multiple second pipelines 82 , thereby realizing the transportation of the samples to be tested.

[0219] Specifically, the second pipeline 82 can be a pipeline for transmitting fluid, and the fluid includes but is not limited to gas, liquid, etc. The cross-sectional shape of the second pipeline 82 can be circular, and the length and diameter of the second pipeline 82 can be set according to actual needs. The number of second pipelines 82 can be consistent with the number of fluid channels 11, and multiple second pipelines 82 are respectively connected to multiple storage devices of the sample box 30 in a one-to-one correspondence, and each storage device is used to store a separate sample to be tested.

[0220] See also Figure 1 In some embodiments, the liquid circuit system 100 includes a third pipeline 83, which is arranged in a one-to-one correspondence with multiple fourth ports 214, and the two ends of the third pipeline 83 are respectively connected to the reagent kit 50 and each fourth port 214, and the third pipeline 83 is used to allow the reagent in the reagent kit 50 to enter each cache area 40 through the first flow path selection valve 21.

[0221] In this way, the reagents in the reagent box 50 can flow from the plurality of fourth ports 214 into the plurality of buffer areas 40 via the third pipeline 83 , thereby realizing the transportation of the reagents.

[0222] Specifically, the third pipeline 83 may be a pipeline for transmitting fluid, the fluid includes but is not limited to gas, liquid, etc. The cross-sectional shape of the third pipeline 83 may be circular, and the length and diameter of the third pipeline 83 may be set according to actual needs. The third pipeline 83 has a liquid inlet 831 and a plurality of liquid outlets 832, the number of which may be consistent with the number of fluid channels 11, the liquid inlet 831 of the third pipeline 83 is connected to the reagent box 50, and the plurality of liquid outlets 832 of the third pipeline 83 are respectively connected to the plurality of fourth ports 214 in a one-to-one correspondence.

[0223] See also Figure 4 and Figure 5 In some embodiments, the first flow path selection valve 21 includes a plurality of communication grooves 215 , and the second port 212 is communicated with one of the first port 211 , the third port 213 , and the fourth port 214 through the communication grooves 215 .

[0224] In this way, the buffer area 40 can be selectively connected to the outlet 13 of the fluid channel 11, the sample box 30 and the reagent box 50 through the connecting groove 215, so as to control the flow path of the sample to be tested and / or the reagent.

[0225] Specifically, the number of the communication grooves 215 may be consistent with the number of the fluid channels 11, and one communication groove 215 corresponds to a group of ports to control the flow of the fluid in one fluid channel 11. The number of the communication grooves 215 may be four, and the four communication grooves 215 may be respectively connected to the four second ports 212 and the four first ports 211, or respectively connected to the four second ports 212 and the four third ports 213, or respectively connected to the four second ports 212 and the four fourth ports 214.

[0226] See also Figure 4 and Figure 5 In some embodiments, the first flow path selection valve 21 includes a stator 216 and a rotor 217 rotatably arranged with the stator 216 , the stator 216 is provided with a plurality of groups of ports, each group of ports includes a first port 211 , a second port 212 , a third port 213 and a fourth port 214 , and the rotor 217 is provided with a connecting groove 215 .

[0227] Thus, by rotating the rotor 217 , the connecting groove 215 can selectively connect the buffer area 40 with the outlet 13 of the fluid channel 11 , the sample box 30 and the reagent box 50 , thereby controlling the switching of the flow path of the sample to be tested and / or the reagent.

[0228] Specifically, the first port 211, the second port 212, the third port 213 and the fourth port 214 may be circular through holes, and the through holes penetrate the stator 216 along the thickness direction of the stator 216. The stator 216 and the rotor 217 may be coaxially arranged, or in other words, the central axis of the stator 216 coincides with the central axis of the rotor 217. The connecting groove 215 may be in a bent shape, a curved shape, etc., and the specific shape of the connecting groove 215 is not limited here.

[0229] See also Figure 4 and Figure 5 In some embodiments, the first port 211 , the second port 212 , the third port 213 , and the fourth port 214 are arranged at intervals along the circumferential direction of the stator 216 .

[0230] Thus, the first port 211 , the second port 212 , the third port 213 and the fourth port 214 are arranged at intervals along the circumferential direction of the stator 216 , so that the second port 212 communicates with one of the first port 211 , the third port 213 and the fourth port 214 through the communication groove 215 .

[0231] Specifically, each group of ports may include a first port 211, two second ports 212, a third port 213 and a fourth port 214, and the first port 211, the third port 213 and the fourth port 214 are respectively distributed on both sides of the two second ports 212, for example, one of the second ports 212 is located between the first port 211 and the third port 213, and the other second port 212 is located between the third port 213 and the fourth port 214. That is to say, the first port 211, the second port 212, the third port 213, the second port 212 and the fourth port 214 are sequentially arranged clockwise or counterclockwise along the circumference of the stator 216. In one embodiment, the two second ports 212 are connected to the same buffer area 40 through a Y-shaped pipeline outside the first flow path selection valve 21, and the first flow path rotary valve 21 is rotated to make the connecting groove 215 connect one of the second ports 212 with the third port 213, or connect the other second port 212 with the fourth port 214. For example, through the Y-shaped pipeline, the reagent, the sample to be tested or the mixture thereof can enter the buffer area 40 through the fourth port 214, the connecting groove 215 and the second port 212, and the reagent, the sample to be tested or the mixture thereof in the buffer area 40 can enter the corresponding fluid channel through the second port 212, the connecting groove 215 and the first port 212. In one embodiment, a plug 218 is provided on the stator 216, and the plug 218 is located on both sides of each group of ports. When the connecting groove 215 is connected to the plug 218 and an adjacent port, the fluid blocking control can be achieved.

[0232] See also Figure 4 and Figure 5 In some embodiments, there are multiple communicating grooves 215 , and the multiple communicating grooves 215 are arranged at intervals along the circumference of the rotor 217 .

[0233] In this way, multiple connecting grooves 215 can simultaneously connect two adjacent ports in multiple groups of ports, and can control multiple fluid paths at the same time, reducing the number of solenoid valves 70 used, thereby reducing the cost and occupied space of the fluid path system 100.

[0234] Specifically, the length of the connecting groove 215 can be the distance between two adjacent ports, so that the connecting groove 215 can connect the two adjacent ports, ensuring that the fluid enters from one port and flows out from one port, achieving accurate control of the fluid flow path, preventing the fluid from entering or flowing out from multiple ports, reducing the risk of cross contamination between different samples to be tested, and improving sequencing quality. The spacing between two adjacent connecting grooves 215 is consistent, so that when one of the connecting grooves 215 is connected to the first port 211 and the second port 212, the remaining connecting grooves 215 can be connected to the corresponding first port 211 and the second port 212.

[0235] See also Figure 1In some embodiments, the second flow path selection valve 23 includes a common port 231 and at least one connecting port 232 , the common port 231 is connected to one end of the third pipeline 83 , and the connecting port 232 is connected to the reagent box 50 .

[0236] In this way, the reagent box 50 and the third pipeline 83 can be connected through the common port 231 and the connecting port 232 to achieve control of the reagent flow path.

[0237] Specifically, there can be two connection ports 232 , one connection port 232 is connected to the reagent box 50 , and the other connection port 232 is connected to the sample box 30 . The common port 231 is selectively connected to one of the connection ports 232 , so that the common port 231 can be selectively connected to the reagent box 50 or the sample box 30 . For example, when the first flow path selection valve 21 connects the cache area 40 and the third pipeline 83, and the second flow path selection valve 23 connects the reagent box 50 and the third pipeline 83, the reagent of the reagent box 50 can flow from the third pipeline 83 into the cache area 40 through the first flow path selection valve 21; when the first flow path selection valve 21 connects the cache area 40 and the first pipeline 81, and the second flow path selection valve 23 connects the reagent box 50 and the manifold assembly 24, the reagent of the reagent box 50 can enter the fluid channel 11 from the inlet 12 of the fluid channel 11 through the manifold assembly 24; when the first flow path selection valve 21 connects the cache area 40 and the first pipeline 81, and the second flow path selection valve 23 connects the sample box 30 and the manifold assembly 24, the sample to be tested in the sample box 30 can enter the fluid channel 11 from the inlet 12 of the fluid channel 11 through the manifold assembly 24.

[0238] The embodiment of the present application provides a sequencing device, including a liquid circuit system 100. In this way, the liquid circuit system 100 can select different injection modes according to the number of samples to be tested, thereby improving the flexibility of loading samples to be tested, while avoiding cross contamination between multiple samples to be tested, and improving sequencing quality and sequencing efficiency.

[0239] An embodiment of the present application provides a computer storage medium. When a computer program is executed by a processor, the processor implements the method for controlling liquid inlet of a liquid circuit system 100 according to any of the above embodiments.

[0240] Specifically, in one embodiment, the processor may be a central processing unit (CPU). The processor may also be other general-purpose processors, digital signal processors (DSP), application-specific integrated circuits (ASIC), field-programmable gate arrays (FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, or a combination of the above chips.

[0241] The computer program can be stored in the memory. The memory, as a non-transitory computer-readable storage medium, can be used to store non-transitory software programs, non-transitory computer executable programs and modules, such as the program instructions / modules corresponding to the methods in the above method embodiments. The processor executes various functional applications and data processing of the processor by running the non-transitory software programs, instructions and modules stored in the memory, that is, realizing the control method in the above method embodiments.

[0242] The storage medium may include, but is not limited to, various media that can store computer programs, such as a USB flash drive, a read-only memory (ROM), a random access memory (RAM), a mobile hard disk, a magnetic disk, or an optical disk.

[0243] In the description of this specification, the description with reference to the terms "one embodiment", "certain embodiments", "illustrative embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials, or characteristics described in conjunction with the embodiments or examples are included in at least one embodiment or example of the utility model. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0244] Although the embodiments of the present invention have been shown and described, those skilled in the art will appreciate that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the claims and their equivalents.

Claims

1. A liquid circuit system, characterized in that: include: A flow cell, wherein the flow cell comprises a plurality of fluid channels, each of the fluid channels is provided with an inlet and an outlet, A pump-valve assembly, the pump-valve assembly is used to draw a plurality of samples to be tested from the sample box, and pump each of the samples to be tested into the fluid channel from an outlet of a corresponding fluid channel; or, The pump-valve assembly is used to draw a single sample to be tested from a sample box, and pump the single sample to be tested into the fluid channel from the inlet of each fluid channel.

2. The fluid circuit system according to claim 1, characterized in that: The pump-valve assembly includes a first flow path selection valve and a pump group, wherein the first flow path selection valve and the pump group are both located downstream of the flow pool, the first flow path selection valve connects the pump group with the fluid channel, and / or connects the pump group with the sample box, and the first flow path selection valve and the pump group are used to draw a plurality of samples to be tested from the sample box, and allow each of the samples to be tested to enter the fluid channel from an outlet of a corresponding fluid channel.

3. The fluid circuit system according to claim 2, characterized in that: The liquid path system includes a plurality of cache areas, and the plurality of cache areas are arranged in one-to-one correspondence with the plurality of fluid channels. The plurality of cache areas are connected to the first flow path selection valve and the pump group. The first flow path selection valve and the pump group pump each of the samples to be tested to a corresponding cache area respectively, and pump the samples to be tested in each cache area into the fluid channel from the outlet of the corresponding fluid channel.

4. The fluid circuit system according to claim 3, characterized in that: The first flow path selection valve is provided with a plurality of first ports and a plurality of second ports, each of the first ports is selectively connected to a second port, the second port is connected to the cache area, the liquid path system comprises a plurality of first pipelines, a plurality of the first pipelines are arranged in one-to-one correspondence with a plurality of the first ports, and both ends of each of the first pipelines are respectively connected to an outlet of the fluid channel and a first port.

5. The fluid circuit system according to claim 4, characterized in that: The first flow path selection valve is provided with a plurality of third ports, each of the third ports is selectively connected to a second port, the liquid path system comprises a plurality of second pipelines, the plurality of second pipelines are arranged in one-to-one correspondence with the plurality of third ports, the two ends of each second pipeline are respectively connected to the sample box and one of the third ports, and each second pipeline is used to allow a sample to be tested in the sample box to enter the corresponding cache area through the first flow path selection valve.

6. The fluid circuit system according to claim 5, characterized in that: The first flow path selection valve is provided with a plurality of fourth ports, each of the fourth ports being selectively connected to a second port, the liquid path system comprises a third pipeline, the third pipeline being arranged in one-to-one correspondence with the plurality of fourth ports, both ends of the third pipeline being respectively connected to the reagent kit and each of the fourth ports, the third pipeline being used to allow the reagent in the reagent kit to enter each of the cache areas through the first flow path selection valve.

7. The fluid circuit system according to claim 6, characterized in that: The first flow path selection valve includes a plurality of communication grooves, and the second port is communicated with one of the first port, the third port, and the fourth port through the communication grooves.

8. The fluid circuit system according to claim 7, characterized in that: The first flow path selection valve includes a stator and a rotor rotatably arranged with the stator, the stator is provided with a plurality of groups of ports, each group of ports includes the first port, the second port, the third port and the fourth port, and the rotor is provided with the communicating groove.

9. The fluid circuit system according to claim 8, characterized in that: The first port, the second port, the third port and the fourth port are arranged at intervals along the circumferential direction of the stator.

10. The fluid circuit system according to claim 8 or 9, characterized in that: There are multiple communicating grooves, and the multiple communicating grooves are arranged at intervals along the circumferential direction of the rotor.

11. The liquid circuit system according to any one of claims 6 to 9, characterized in that: The pump-valve assembly includes a second flow path selection valve, which is located upstream of the flow pool. The second flow path selection valve includes a common port and at least one connecting port. The common port is connected to one end of the third pipeline, and the connecting port is connected to the reagent kit. The second flow path selection valve is used for the pump-valve assembly to draw reagents from the reagent kit and pump the reagents to each of the cache areas, and pump the reagents in each of the cache areas into the fluid channel from the outlet of the corresponding fluid channel.

12. The fluid circuit system according to claim 11, characterized in that: The pump valve assembly is used to pump the sample to be tested and / or the reagent to the buffer area in a negative pressure driven manner.

13. The fluid circuit system according to claim 11, characterized in that: The pump-valve assembly is used to pump the sample to be tested and / or the reagent in the buffer area from the outlet of the fluid channel into the fluid channel in a positive pressure-driven manner.

14. The fluid circuit system according to any one of claims 6 to 9, characterized in that: The pump-valve assembly includes a second flow path selection valve, which is located upstream of the flow pool. The second flow path selection valve includes a common port and at least one connecting port. The common port is connected to one end of the third pipeline, and the connecting port is connected to the test kit. The second flow path selection valve is used for the pump-valve assembly to draw reagents from the test kit and pump the reagents into the sample box to mix with each of the samples to be tested in the sample box.

15. The fluid circuit system according to claim 14, characterized in that: The pump valve assembly is used to pump the reagent to each of the buffer areas, and pump the reagent in each of the buffer areas into the sample box to mix with the corresponding sample to be tested in the sample box.

16. The fluid circuit system according to claim 15, characterized in that: The pump valve assembly is used to pump the mixture of each sample to be tested and the reagent back to the corresponding buffer area, and pump the mixture of the sample to be tested and the reagent in each buffer area into the fluid channel from the outlet of the corresponding fluid channel.

17. The fluid circuit system according to claim 16, characterized in that: The pump valve assembly is used to pump the mixed solution of the sample to be tested and the reagent to the buffer area in a negative pressure driven manner.

18. The fluid circuit system according to claim 16 or 17, characterized in that: The pump-valve assembly is used to pump the mixed solution of the sample to be tested and the reagent in the buffer area from the outlet of the fluid channel into the fluid channel in a positive pressure-driven manner.

19. The fluid circuit system according to claim 11, characterized in that: The pump-valve assembly includes a manifold assembly, which is located upstream of the flow pool. The second flow path selection valve connects the reagent box with the manifold assembly, and / or connects the reagent box with the first flow path selection valve. The manifold assembly connects the common port and each fluid channel of the flow pool. The second flow path selection valve, the manifold assembly and the pump group are used to draw a single sample to be tested and / or a reagent from the sample box and / or the reagent box, and allow the single sample to be tested and / or the reagent to enter the fluid channel from the inlet of each fluid channel.

20. A sequencing device, characterized in that: A liquid circuit system comprising any one of claims 1-19.

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