A high-throughput fully automatic nucleic acid extraction workstation

Abstract

The utility model discloses a high-throughput fully automatic nucleic acid extraction workstation, which relates to the technical field of workstations, including a workbench, an automatic centrifuge is arranged on the rear side of one end of the workbench, and a handling manipulator is arranged on the top surface of the workbench on the side of the automatic centrifuge, and an automatic film sealing instrument is arranged on the workbench on the front side of the automatic centrifuge. The utility model uses an automated extraction hole workbench to automatically complete the extraction operations of adding lysate, sealing, heating and shaking, adding clarification reagent, standing, centrifuging, pipetting, and well plate collection during extraction, solving the problems of the existing nucleic acid extraction being time-consuming and labor-intensive and having many consumables, and the manual extraction of nucleic acid being time-consuming, inefficient, having a large labor input cost, high occupational safety risks, and uncontrollable quality of the obtained nucleic acid. It breaks away from the framework of traditional nucleic acid extraction and can automatically obtain purified nucleic acid in only one step.

Description

Technical Field

[0001] The utility model relates to the technical field of workstations, in particular to a high-throughput fully automatic nucleic acid extraction workstation. Background Art

[0002] Nucleic acid is a general term for deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). It is a biological macromolecular compound composed of many nucleotide monomers and is one of the most basic substances of life. Nucleic acid extraction plays a vital role in molecular biology. The extracted nucleic acid can be used for various downstream applications such as subsequent cloning, polymerase chain reaction, real-time fluorescence quantitative nucleic acid amplification detection system, library construction and sequencing to conduct biological related research. Nucleic acid extraction generally includes three steps: (1) releasing nucleic acids through cell lysis; (2) removing membrane lipids, proteins and other impurities; (3) obtaining nucleic acids through separation to complete nucleic acid collection.

[0003] However, in the prior art, when nucleic acid is extracted, whether it is the chemical reagent method, column extraction method or magnetic bead method, it is necessary to go through multiple steps of "lysis-washing-elution", which is time-consuming and labor-intensive, uses a lot of disposable consumables, and when nucleic acid is extracted manually, it takes a long time, has low work efficiency, high labor input costs, high occupational safety risks, and the quality of the obtained nucleic acid is uncontrollable. Utility Model Content

[0004] The utility model aims to solve the problems existing in the prior art and proposes a high-throughput fully automatic nucleic acid extraction workstation.

[0005] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: comprising a workbench, an automatic centrifuge is arranged at the rear side of one end of the workbench, a handling manipulator is arranged on the top surface of the workbench on the side of the automatic centrifuge, and an automatic film sealing instrument is arranged on the workbench on the front side of the automatic centrifuge;

[0006] An XYZ three-axis moving table is arranged on the top surface of the workbench of the transport robot away from the automatic centrifuge side, and a sample well plate is arranged on the top surface of the workbench in front of the XYZ three-axis moving table, a finished product well plate is arranged on the top surface of the workbench in front of the sample well plate, a mixed liquid well plate is arranged on the side of the sample well plate away from the automatic centrifuge, a transfer well plate is arranged on the side of the finished product well plate away from the automatic film sealing instrument, a material preparation well plate is arranged on the top surface of the workbench on the sides of the transfer well plate and the mixed liquid well plate away from the automatic film sealing instrument, and a pipette tip is loaded inside the material preparation well plate, and a 96-channel pipette is arranged at the output end of the XYZ three-axis moving table.

[0007] Preferably, a protective cover is provided on the fixed cover outside the workbench, an observation window which can be rotated upward to open is provided on the front side of the protective cover on the top surface of the workbench, and a handle is fixed on the middle part of the lower front side of the observation window.

[0008] Preferably, an exhaust window is provided in the middle of the top surface of the protective cover, and the upper and lower ends of the exhaust window are respectively connected with the inner and outer sides of the protective cover, and a transparent plate is embedded in the middle of the observation window.

[0009] Preferably, a controller is provided in the middle of the protective cover above the observation window, and the output end of the controller is electrically connected to the automatic centrifuge, automatic film sealing instrument, handling robot, oscillation heating module, metal refrigeration module, XYZ three-axis moving table and 96-channel pipette input end.

[0010] Preferably, the input end of the automatic film sealing instrument is provided with a well plate clamping mechanism for clamping and fixing the well plate, the bottom surface of the well plate containing the sample is provided with an oscillating heating module, and the oscillating heating module is installed on the top surface of the workbench, and the oscillating heating module is used for oscillating and heating the lysate.

[0011] Preferably, a metal refrigeration module is provided on the bottom surface of the mixed liquid orifice plate, and the bottom surface of the metal refrigeration module is installed on the top surface of the workbench. The refrigeration temperature of the metal refrigeration module is ° C, which is used to store the lysate and the clarified liquid at low temperature.

[0012] Compared with the prior art, the advantages and positive effects of the utility model are:

[0013] 1. In the utility model, through the automated extraction well workbench, during extraction, the extraction operations of adding lysis solution, sealing, heating and shaking, adding clarification reagent, standing, centrifuging, pipetting, and well plate collection are automatically completed, which solves the problems that the existing nucleic acid extraction is time-consuming and labor-intensive and has many consumables, and manual nucleic acid extraction is time-consuming, inefficient, has high labor input costs, high occupational safety risks, and the quality of the obtained nucleic acid is uncontrollable. It breaks away from the framework of traditional nucleic acid extraction and can automatically obtain purified nucleic acid in only one step.

[0014] 2. In the utility model, the handling manipulator and the XYZ platform are coordinated to ensure that the pipette tip is more stable during operation when extracting nucleic acid. In conjunction with the automatic film sealing instrument, after extraction, the finished well plate is sealed and preserved by sealing, making the well plate more convenient to handle and preventing internal liquid from leaking. The well plate is fixed by the well plate clamping mechanism, so that after the 96-channel pipette is equipped with the pipette tip, it must pass through the sealed membrane to transfer liquid into the well plate. After the well plate is clamped, the well plate will not be lifted up when the pipette is withdrawn. At the same time, the protective cover can reduce the risk of contamination during extraction, and the observation window is used to facilitate observation of the operating status. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 The utility model provides a structural schematic diagram of a fully automatic nucleic acid extraction workstation;

[0016] Figure 2This is a left view schematic diagram of the internal structure of the fully automatic nucleic acid extraction workstation proposed by the utility model;

[0017] Figure 3 This is a right view schematic diagram of the internal structure of the fully automatic nucleic acid extraction workstation proposed by the utility model;

[0018] Figure 4 This is a schematic diagram of the internal structure of the fully automatic nucleic acid extraction workstation proposed by the utility model from a bottom-up perspective;

[0019] Figure 5 The utility model provides a schematic diagram of the internal structure of the fully automatic nucleic acid extraction workstation from the back.

[0020] Legend: 1. Workbench; 2. Protective cover; 3. Controller; 4. Observation window; 5. Exhaust window; 6. Automatic centrifuge; 7. Automatic film sealing instrument; 8. Handling robot; 9. Sample well plate; 10. Mixing liquid well plate; 11. Oscillating heating module; 12. Metal refrigeration module; 13. Preparation well plate; 14. XYZ three-axis moving stage; 15. 96-channel pipette; 16. Pipette tip; 17. Transfer well plate; 18. Finished well plate; 19. Well plate clamping mechanism. DETAILED DESCRIPTION

[0021] In order to more clearly understand the above-mentioned purpose, features and advantages of the utility model, the utility model is further described below in conjunction with the accompanying drawings and embodiments. It should be noted that the embodiments of the present application and the features in the embodiments can be combined with each other without conflict.

[0022] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways than those described herein. Therefore, the present invention is not limited to the specific embodiments of the following disclosure.

[0023] Embodiment 1

[0024] like Figure 1-5As shown, the utility model provides a high-throughput fully automatic nucleic acid extraction workstation, including a workbench 1, a protective cover 2 is provided on the fixed cover outside the workbench 1, an observation window 4 that can be rotated upward to open is provided on the front side of the protective cover 2 on the top surface of the workbench 1, and a handle is fixed in the middle of the lower front side of the observation window 4, a transparent plate is embedded in the middle of the observation window 4, an exhaust window 5 is provided in the middle of the top surface of the protective cover 2, and the upper and lower ends of the exhaust window 5 are respectively connected with the inner and outer sides of the protective cover 2, and an automatic centrifuge 6 is provided on the rear side of one end of the workbench 1. The automatic centrifuge 6 uses a high-speed rotating centrifugal rotor to act on the sample to generate a large centrifugal force, so that substances of different densities are subjected to different centrifugal forces in the tube or the centrifugal chamber, thereby realizing the separation of substances, and the automatic centrifuge 6 A handling manipulator 8 is arranged on the top surface of the workbench 1 on the side. The handling manipulator 8 is controlled by PLC + touch screen + servo motor. When the handling manipulator 8 is in operation, the rising, lowering, leftward and rightward grasping and releasing operations are all completed by a double-coil three-position solenoid valve pneumatic cylinder. When a certain solenoid valve is energized, the corresponding action is maintained, and it is still maintained even if the coil is powered off again until the coil in the opposite direction is energized, and the corresponding action is completed. The equipment is equipped with six limit switches of up, down, left, right, grasping and releasing to control the end of the corresponding working steps. An automatic film sealing instrument 7 is arranged on the workbench 1 on the front side of the automatic centrifuge 6. The automatic film sealing instrument 7 is composed of a frame, a transmission system, a sealing head and a control module, etc., and can automatically complete the entire sealing process;

[0025] The top surface of the workbench 1 on the side of the transport robot 8 away from the automatic centrifuge 6 is provided with an XYZ three-axis moving table 14, and the top surface of the workbench 1 in front of the XYZ three-axis moving table 14 is provided with a sample well plate 9, and the top surface of the workbench 1 in front of the sample well plate 9 is provided with a finished product well plate 18, and the side of the sample well plate 9 away from the automatic centrifuge 6 is provided with a mixed liquid well plate 10, and the side of the finished product well plate 18 away from the automatic film sealing instrument 7 is provided with a transfer well plate 17, and the transfer well plate 17 and the mixed liquid well plate 10 are away from the automatic centrifuge 6. A material preparation orifice plate 13 is arranged on the top surface of the workbench 1 on the side of the dynamic film sealing instrument 7, and a pipette tip 16 is loaded inside the material preparation orifice plate 13, a 96-channel pipette 15 is arranged at the output end of the XYZ three-axis moving table 14, and a controller 3 is arranged in the middle of the protective cover 2 above the observation window 4, and the output end of the controller 3 is electrically connected to the input end of the automatic centrifuge 6, the automatic film sealing instrument 7, the handling robot 8, the oscillation heating module 11, the metal refrigeration module 12, the XYZ three-axis moving table 14 and the 96-channel pipette 15.

[0026] The specific configuration and function of this embodiment are described in detail below: the input end of the automatic film sealing instrument 7 is provided with a well plate clamping mechanism 19 for clamping and fixing the well plate. The function of the well plate clamping mechanism 19 is as follows: when the well plate after the film is sealed is added with liquid, the 96-channel pipette 15 is equipped with a pipette tip 16 to pass through the sealed film to transfer liquid into the well plate. After the well plate is clamped, the pipette tip 16 will not lift the well plate when it is withdrawn. An oscillation heating module 11 is provided on the bottom surface of the well plate 9 containing the sample, and the oscillation heating module 11 is installed on the top surface of the workbench 1, wherein the oscillation heating module 11 is composed of a heating tube and an oscillation generator. The oscillation heating module 11 It is used for oscillating and heating the lysate. A metal refrigeration module 12 is arranged on the bottom surface of the mixed liquid orifice plate 10, and the bottom surface of the metal refrigeration module 12 is installed on the top surface of the workbench 1. The metal refrigeration module 12, i.e., the metal material is in the magnetic field, and its free electrons are affected by the magnetic field, and spin polarization and magnetic moment change will occur, thereby generating a magnetic enthalpy change. When the metal material is under appropriate temperature and magnetic field conditions, the magnetic enthalpy change will cause the temperature of the material to drop, thereby achieving a refrigeration effect and accelerating the cooling effect of the mixed liquid orifice plate 10. The refrigeration temperature of the metal refrigeration module 12 is 4°C, which is used for low-temperature storage of lysate and clarified liquid.

[0027] The use method and working principle of the present device are as follows: the transport robot 8 is operated to load the sample well plate 9 onto the oscillating heating module 11, at which time the XYZ three-axis moving stage 14 is operated to drive the 96-channel pipette 15 to move, and the lysate is added into the wells of the sample well plate 9 one by one through the 96-channel pipette 15, the transport robot 8 is operated again, and the sample well plate 9 with the lysate added is placed in the automatic film sealing instrument 7 for film sealing, after the film sealing, the transport robot 8 moves the sample well plate 9 back to the oscillating heating module 11, and turns on the oscillating heating module 11, so that the oscillating heating module 11 is operated to heat and oscillate the sample well plate 9, after the processing is completed, the XYZ three-axis moving stage 14 is operated again, driving the 96-channel pipette 15 to add clarification reagent to the sample well plate 9 after heating and oscillation, at which time the sample well plate 9 becomes a mixed solution well plate 10, and then the transport robot 8 is operated to add the mixed solution well plate 10 with the clarification reagent The plate 10 is placed above the metal refrigeration module 12. At this time, the metal refrigeration module 12 operates to cool the mixed liquid orifice plate 10, and it is allowed to stand while cooling. After the standing is completed, the handling robot 8 operates to place the mixed liquid orifice plate 10 in the automatic centrifuge 6 for centrifugal treatment, and then transfers the liquid after treatment. During transfer, the liquid in the mixed liquid orifice plate 10 is first received by the transfer orifice plate 17, and then the transfer orifice plate 17 is moved by the handling robot 8, and the transfer orifice plate 17 is placed in the automatic centrifuge 6, so that the liquid in the transfer orifice plate 17 can be centrifuged. After centrifugation, the XYZ three-axis moving table 14 operates to move the liquid in the transfer orifice plate 17 into the finished product orifice plate 18 through the 96-channel pipette 15, and the transfer orifice plate 17 is discarded. After the extract is filled into the finished product orifice plate 18, the handling robot 8 operates to move the finished product orifice plate 18 into the automatic film sealing instrument 7 for film sealing, and finally the processed orifice plate is collected.

[0028] The above description is only a preferred embodiment of the present invention and does not limit the present invention in other forms. Any technician familiar with the profession may use the technical content disclosed above to change or modify it into an equivalent embodiment with equivalent changes and apply it to other fields. However, any simple modification, equivalent change and modification made to the above embodiment based on the technical essence of the present invention without departing from the content of the technical solution of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. A high-throughput fully automatic nucleic acid extraction workstation, comprising a workbench (1), characterized in that: An automatic centrifuge (6) is arranged at the rear side of one end of the workbench (1), and a handling robot (8) is arranged on the top surface of the workbench (1) on the side of the automatic centrifuge (6), and an automatic film sealing instrument (7) is arranged on the workbench (1) on the front side of the automatic centrifuge (6); An XYZ three-axis moving table (14) is arranged on the top surface of the workbench (1) of the transport robot (8) away from the automatic centrifuge (6), and a sample well plate (9) is arranged on the top surface of the workbench (1) in front of the XYZ three-axis moving table (14), and a finished product well plate (18) is arranged on the top surface of the workbench (1) in front of the sample well plate (9), and a mixed liquid well plate (10) is arranged on the side of the sample well plate (9) away from the automatic centrifuge (6), and a transfer well plate (17) is arranged on the side of the finished product well plate (18) away from the automatic film sealing instrument (7), and a material preparation well plate (13) is arranged on the top surface of the workbench (1) on the side of the transfer well plate (17) and the mixed liquid well plate (10) away from the automatic film sealing instrument (7), and a suction head (16) is loaded inside the material preparation well plate (13), and a 96-channel pipette (15) is arranged at the output end of the XYZ three-axis moving table (14).

2. A high-throughput fully automatic nucleic acid extraction workstation according to claim 1, characterized in that: A protective cover (2) is provided on the fixed cover outside the workbench (1), an observation window (4) that can be rotated upwards and opened is provided on the front side of the protective cover (2) on the top surface of the workbench (1), and a handle is fixed in the middle of the lower front side of the observation window (4).

3. A high-throughput fully automatic nucleic acid extraction workstation according to claim 2, characterized in that: An exhaust window (5) is provided in the middle of the top surface of the protective cover (2), and the upper and lower ends of the exhaust window (5) are respectively connected to the inner and outer sides of the protective cover (2), and a transparent plate is embedded in the middle of the observation window (4).

4. A high-throughput fully automatic nucleic acid extraction workstation according to claim 2, characterized in that: A controller (3) is provided in the middle of the protective cover (2) above the observation window (4), and the output end of the controller (3) is electrically connected to the input ends of the automatic centrifuge (6), the automatic film sealing instrument (7), the handling robot (8), the oscillating heating module (11), the metal refrigeration module (12), the XYZ three-axis moving table (14) and the 96-channel pipette (15).

5. A high-throughput fully automatic nucleic acid extraction workstation according to claim 1, characterized in that: The input end of the automatic film sealing instrument (7) is provided with a well plate clamping mechanism (19) for clamping and fixing the well plate. The bottom surface of the well plate (9) containing the sample is provided with an oscillating heating module (11), and the oscillating heating module (11) is installed on the top surface of the workbench (1). The oscillating heating module (11) is used for oscillating and heating the lysate.

6. A high-throughput fully automatic nucleic acid extraction workstation according to claim 1, characterized in that: The bottom surface of the mixed liquid orifice plate (10) is provided with a metal refrigeration module (12), and the bottom surface of the metal refrigeration module (12) is installed on the top surface of the workbench (1). The refrigeration temperature of the metal refrigeration module (12) is 4° C., and is used for low-temperature storage of lysate and clarified liquid.

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