A device for extracting and collecting exosomes

Abstract

The utility model provides an exosome extraction and collection device, including a base and a mixing cylinder and a filter box fixedly installed on the top of the base from left to right in sequence, wherein a frame plate is slidably connected in the slide rail, a filter screen is arranged on the top of the frame plate, a driving gear ring is fixedly installed on one side of the top of the frame plate through a base block, and a half gear meshing with the driving gear ring is arranged in the driving gear ring; one end of the shaft rod passes through the filter box. The driving mechanism drives the half gear to rotate, so that the half gear rotates in the driving gear ring, and drives it to move back and forth, so that the driving gear ring drives the frame plate to slide back and forth in the slide rail, thereby driving the filter screen to move back and forth, so as to disperse the exosomes and change their positions to break the blocked filter holes, thereby preventing the exosomes from aggregating and depositing on the filter screen to cause the filter holes to be blocked, thereby achieving the anti-blocking effect of the filter screen, and effectively improving the filtering and extraction effect and efficiency of the exosomes.

Description

Technical Field

[0001] The utility model relates to the technical field of exosome extraction, and in particular to an exosome extraction and collection device. Background Art

[0002] Exosomes refer to small membrane vesicles (30-150nm) containing complex RNA and proteins. Nowadays, they specifically refer to discoid vesicles with a diameter of 40-100nm. They are mainly derived from multivesicular bodies formed by the invagination of lysosomal microparticles in cells, and are released into the extracellular matrix after the multivesicular outer membrane fuses with the cell membrane.

[0003] The known patent application number is: CN202222597937.4, which discloses a filtering and extracting device for stem cell exosomes, including a base, a mixing box is fixedly installed on the left side of the top of the base, a filter box is fixedly installed on the right side of the top of the base, and support rods are fixedly installed on the left and right sides of the top of the mixing box. The utility model drives the stirring rod to rotate by the output end of the rotating motor, and the stirring rod drives the stirring blade to rotate, so as to stir and mix the sample and the diluent in the inner cavity of the mixing box. At this time, the output end of the servo motor drives the screw to rotate, the screw drives the screw sleeve to move left and right, the screw sleeve drives the fixed box to move left and right through the connecting rod, and the fixed box drives the stirring rod and the stirring blade to move left and right, so as to accelerate the mixing effect of the sample and the diluent. After mixing, the mixed sample is sucked into the inner cavity of the filter box through the input end of the water pump through the conveying pipe. At this time, the sample is filtered through the filter screen and discharged through the discharge pipe.

[0004] However, in the implementation of relevant technologies, it was found that the above scheme has the following problems: when it is used, it is not convenient to prevent the filter from being blocked. During the filtration and extraction of exosomes through the filter, since the exosomes have a certain viscosity, when the exosomes pass through the filter, the viscous components therein will attract each other and gather together and adhere to the pore size or surface of the filter, causing the filter to be blocked, thereby reducing the filtration and extraction effect and efficiency of the exosomes. Utility Model Content

[0005] In order to solve the above problems, the present invention proposes an exosome extraction and collection device. The technical solution solves the problem that it is inconvenient to prevent the filter from being blocked as mentioned in the above background technology.

[0006] To achieve the above-mentioned purpose, the utility model provides an exosome extraction and collection device, comprising a base and a mixing cylinder and a filter box fixedly installed on the top of the base from left to right, a conveying member is provided between the mixing cylinder and the filter box, and a mixing mechanism is provided in the mixing cylinder;

[0007] Slide rails are symmetrically fixedly installed on both sides of the inner wall of the filter box, and a frame plate is slidably connected inside the slide rail. A filter screen is arranged above the frame plate. A driving gear ring is fixedly installed on one side of the top of the frame plate through a base block, and a half gear meshing with the driving gear ring is arranged inside the driving gear ring.

[0008] A telescopic mechanism for moving the filter screen is provided at the bottom of the frame plate, and a shaft is rotatably connected between the two sides of the inner wall of the filter box and below the frame plate, and cams cooperating with the telescopic mechanism are fixedly installed on the surfaces of both ends of the shaft;

[0009] A power mechanism is provided on one side of the filter box, which is used to synchronously drive the half gear and the shaft rod to rotate, and one end of the shaft rod passes through the filter box.

[0010] Optionally, the telescopic mechanism includes a sliding rod that is slidably connected to the four corners of the surface of the frame plate, a movable plate that is fixedly connected to the bottom end of the sliding rod and in contact with the cam, and a spring that is sleeved on the surface of the sliding rod, the two ends of the spring are respectively connected to the frame plate and the movable plate, and the filter is fixedly connected to the top of the sliding rod.

[0011] Optionally, the power mechanism includes a motor 1 fixedly mounted on a side of a filter box through a bracket and two pulleys fixedly connected to the output end of the motor 1 and one end of a shaft rod. The output end of the motor 1 passes through the filter box and is connected to a half gear, and the two pulleys are connected by a belt tensioning connection.

[0012] Optionally, the mixing mechanism includes a shell fixedly mounted on the top of the mixing barrel and connected thereto, a moving component arranged in the shell, a second motor arranged at the output end of the moving component, and a stirring shaft fixedly connected to the output end of the second motor.

[0013] The moving component is composed of a reciprocating screw rotatably connected between the two sides of the inner wall of the shell, a guide rod fixedly installed between the two sides of the inner wall of the shell, a slider threadedly connected to the surface of the reciprocating screw and sliding with the guide rod, and a driving member arranged on one side of the shell, and the motor 2 is fixedly installed at the bottom of the slider.

[0014] The driving member is composed of a motor 3 fixedly mounted on a side surface of the housing, and the output end of the motor 3 passes through the housing and is connected to the reciprocating screw.

[0015] Optionally, the conveying member is composed of a pump body fixedly installed on a side of the filter box, the input end of the pump body is connected to the mixing cylinder through pipeline one, and the output end of the pump body extends into the filter box through pipeline two.

[0016] Optionally, a feed pipe is fixedly mounted on the top of the mixing cylinder, a box cover is hingedly connected to the top of the filter box, and a collection drawer box is disposed on the inner bottom wall of the filter box.

[0017] Compared with the prior art, the beneficial effects of the utility model are:

[0018] 1. When the utility model is used, the sample and the diluent enter the mixing cylinder, and the sample and the diluent are fully stirred and mixed by the mixing mechanism to facilitate the subsequent extraction of exosomes. The mixed sample is then transported to the filter box by the conveying member for filtration and extraction through the filter. During the process, the driving mechanism drives the half gear to rotate, so that the half gear rotates in the driving gear ring and drives it to move back and forth, so that the driving gear ring drives the frame plate to slide back and forth in the slide rail, thereby driving the filter to move back and forth, so as to disperse the exosomes and change their position to break the blocked filter holes, thereby preventing the exosomes from aggregating and depositing on the filter net to cause the filter holes to be blocked, thereby achieving the anti-blocking effect of the filter net, and effectively improving the filtering and extraction effect and efficiency of the exosomes.

[0019] 2. The utility model drives the shaft rod and the cam to rotate through the driving mechanism. When the raised end of the cam contacts the movable plate, the movable plate and the slide rod are driven to move upward to compress the spring, thereby driving the filter to move upward. Conversely, the spring drives the movable plate and the slide rod to move downward and reset, thereby achieving the effect of reciprocating movement of the filter up and down to generate vibration, so as to further destroy the aggregation of the sample, so that it can be filtered through the filter in a more dispersed manner, preventing the filter from being blocked, and greatly improving the filtration and extraction efficiency of exosomes.

[0020] In order to better understand and implement the present invention, the present invention is described in detail below with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0022] Figure 2 This is a schematic diagram of the cross-sectional structure of the filter box of the utility model;

[0023] Figure 3 It is a schematic diagram of the partial structure of the frame plate of the utility model;

[0024] Figure 4 It is a schematic diagram of the cross-sectional structure of the shell of the utility model.

[0025] In the figure: 1. base; 2. mixing cylinder; 3. filter box; 4. slide rail; 5. frame plate; 6. filter screen; 7. drive gear ring; 8. half gear; 9. shaft; 10. cam; 11. slide bar; 12. movable plate; 13. spring; 14. motor one; 15. pulley; 16. housing; 17. motor two; 18. stirring shaft; 19. reciprocating screw; 20. guide rod; 21. motor three; 22. pump body; 23. collection drawer box. DETAILED DESCRIPTION

[0026] In order to have a clearer understanding of the technical features, purposes and effects of the utility model, the specific implementation methods of the utility model are now described with reference to the accompanying drawings, but the protection scope of the utility model is not limited to the following.

[0027] Reference Figure 1-4 As shown, the exosome extraction and collection device provided by the utility model solves the problem of inconvenience in anti-clogging treatment of the filter screen raised in the above-mentioned background technology.

[0028] To achieve the above-mentioned purpose, an exosome extraction and collection device comprises a base 1, and a mixing cylinder 2 and a filter box 3 fixedly mounted on the top of the base 1 from left to right, a conveying member is provided between the mixing cylinder 2 and the filter box 3, and a mixing mechanism is provided in the mixing cylinder 2;

[0029] Slide rails 4 are symmetrically fixedly installed on both sides of the inner wall of the filter box 3, and a frame plate 5 is slidably connected in the slide rails 4. A filter screen 6 is arranged above the frame plate 5. A driving gear ring 7 is fixedly installed on one side of the top of the frame plate 5 through a base block. A half gear 8 meshing with the driving gear ring 7 is arranged in the driving gear ring 7, wherein the driving gear ring 7 is a straight notch structure, and a plurality of teeth cooperating with the half gear 8 are integrally formed in a staggered state on the upper and lower side surfaces of the inner wall;

[0030] A telescopic mechanism for moving the filter screen 6 is provided at the bottom of the frame plate 5. A shaft 9 is rotatably connected between the two sides of the inner wall of the filter box 3 and located below the frame plate 5. Cams 10 cooperating with the telescopic mechanism are fixedly installed on the surfaces of both ends of the shaft 9.

[0031] A power mechanism is provided on one side of the filter box 3 , which is used to synchronously drive the half gear 8 and the shaft rod 9 to rotate, and one end of the shaft rod 9 passes through the filter box 3 .

[0032] The utility model provides an exosome extraction and collection device. When in use, a sample and a diluent enter a mixing barrel 2, and the sample and the diluent are fully stirred and mixed by a mixing mechanism to facilitate subsequent extraction of exosomes. The mixed sample is then transported to a filter box 3 by a transport member for filtration and extraction by a filter screen 6. During the process, the driving mechanism drives the half gear 8 and the shaft 9 to rotate synchronously, so that the half gear 8 rotates in a driving gear ring 7, and drives it to move back and forth, so that the driving gear ring 7 drives the frame plate 5 to slide back and forth in a slide rail 4, thereby driving the filter screen 6 to move back and forth, so as to disperse the exosomes and change their positions to break the blocked filter holes, thereby preventing the exosomes from aggregating and depositing on the filter screen 6 to cause the filter holes to be blocked, thereby achieving the anti-blocking effect of the filter screen 6, and effectively improving the filtering and extraction effect and efficiency of the exosomes. At the same time, the shaft 9 drives the cam 10 to rotate, so that the cam 10 cooperates with the telescopic mechanism to make the filter screen 6 move back and forth up and down to vibrate, thereby achieving the effect of the filter screen 6 moving back and forth and vibrating at the same time, further improving the filtering efficiency of the exosomes.

[0033] Furthermore, the telescopic mechanism includes a sliding rod 11 that is slidably connected to the four corners of the surface of the frame plate 5, a movable plate 12 that is fixedly connected to the bottom end of the sliding rod 11 and in contact with the cam 10, and a spring 13 that is sleeved on the surface of the sliding rod 11. The two ends of the spring 13 are respectively connected to the frame plate 5 and the movable plate 12, and the filter 6 is fixedly connected to the top of the sliding rod 11.

[0034] Specifically, during the rotation of the shaft 9, when the raised end of the cam 10 contacts the movable plate 12, it can drive the movable plate 12 and the slide bar 11 to move upward to compress the spring 13, thereby driving the filter 6 to move upward. When the raised end of the cam 10 is no longer in contact with the movable plate 12, the spring 13 drives the movable plate 12 and the slide bar 11 to move downward and reset, thereby achieving the effect of reciprocating up and down movement of the filter 6 to vibrate, so as to further destroy the aggregation of the sample, so that it can be filtered through the filter 6 in a more dispersed manner, preventing the filter 6 from being blocked, and greatly improving the filtration and extraction efficiency of exosomes.

[0035] It should be noted that the vibration intensity generated by the cam 10 in cooperation with the spring 13 is moderate and will not cause damage or influence to the sample.

[0036] Furthermore, the power mechanism includes a motor 14 fixedly mounted on a side of the filter box 3 through a bracket and two pulleys 15 fixedly connected to the output end of the motor 14 and one end of the shaft 9. The output end of the motor 14 passes through the filter box 3 and is connected to the half gear 8, and the two pulleys 15 are connected by a belt tensioning connection.

[0037] Specifically, during the filtering process, the motor 14 can drive the pulley 15 and the half gear 8 at its output end to rotate, thereby driving the filter 6 to move back and forth through the cooperation of the half gear 8 and the driving gear ring 7. At the same time, under the action of the belt, the shaft 9 and the pulley 15 at its end are driven to rotate, thereby driving the cam 10 to cooperate with the telescopic mechanism to make the filter 6 move up and down and vibrate, and then the filter 6 can vibrate while moving back and forth, further reducing the clogging of the filter 6.

[0038] Furthermore, the mixing mechanism includes a shell 16 fixedly mounted on the top of the mixing barrel 2 and connected thereto, a moving component arranged in the shell 16, a motor 17 arranged at the output end of the moving component, and a stirring shaft 18 fixedly connected to the output end of the motor 17.

[0039] The moving part is composed of a reciprocating screw 19 rotatably connected between the two sides of the inner wall of the shell 16, a guide rod 20 fixedly installed between the two sides of the inner wall of the shell 16, a slider threadedly connected to the surface of the reciprocating screw 19 and sliding with the guide rod 20, and a driving member arranged on one side of the shell 16, and a motor 17 is fixedly installed at the bottom of the slider.

[0040] The driving member is composed of a motor 3 21 fixedly mounted on a side of the housing 16 , and the output end of the motor 3 21 passes through the housing 16 and is connected to the reciprocating screw 19 .

[0041] Specifically, during mixing, the stirring shaft 18 is driven by the motor 2 17 to rotate to stir and mix the cell sample and the diluent, and at the same time, the reciprocating screw 19 is driven by the motor 3 21 to rotate, so that the slider drives the motor 2 17 to move back and forth laterally along the guide rod 20, thereby driving the stirring shaft 18 to move, so as to expand the stirring and mixing range and reduce the mixing dead angle.

[0042] Furthermore, the conveying member is composed of a pump body 22 fixedly mounted on a side of the filter box 3, the input end of the pump body 22 is connected to the mixing cylinder 2 through a pipe 1, and the output end of the pump body 22 extends to the filter box 3 through a pipe 2.

[0043] Specifically, by providing the pump body 22 , it is convenient to extract and transport the mixed sample in the mixing cylinder 2 to the filter box 3 for filtering and extraction processing.

[0044] Furthermore, a feed pipe is fixedly installed on the top of the mixing barrel 2, and a sealing plug is provided in its port. A box cover is hinged on the top of the filter box 3, and a collection drawer box 23 is provided on the inner bottom wall of the filter box 3. A slot is penetrated through one side of the filter box 3 to facilitate the pulling out of the collection drawer box 23.

[0045] Specifically, by providing a feeding tube, it is convenient to feed the cell sample and the diluent into the mixing barrel 2 for processing, and by providing a collecting drawer box 23, it is convenient to collect the extracted exosomes for subsequent removal and use.

[0046] The above disclosure is only a preferred embodiment of the present invention, which certainly cannot be used to limit the scope of rights of the present invention. Therefore, equivalent changes made according to the claims of the present invention still fall within the scope covered by the present invention.

Claims

1. An exosome extraction and collection device, characterized in that: It comprises a base (1) and a mixing cylinder (2) and a filter box (3) fixedly mounted on the top of the base from left to right in sequence, a conveying member is provided between the mixing cylinder (2) and the filter box (3), and a mixing mechanism is provided inside the mixing cylinder (2); Slide rails (4) are symmetrically fixedly installed on both sides of the inner wall of the filter box (3), a frame plate (5) is slidably connected inside the slide rail (4), a filter screen (6) is provided above the frame plate (5), a driving gear ring (7) is fixedly installed on one side of the top of the frame plate (5) through a base block, and a half gear (8) meshing with the driving gear ring (7) is provided inside the driving gear ring (7); A telescopic mechanism for moving the filter screen (6) is provided at the bottom of the frame plate (5); a shaft rod (9) is rotatably connected between the two sides of the inner wall of the filter box (3) and located below the frame plate (5); cams (10) cooperating with the telescopic mechanism are fixedly mounted on the surfaces of both ends of the shaft rod (9); A power mechanism is provided on one side of the filter box (3), which is used to synchronously drive the half gear (8) and the shaft rod (9) to rotate, and one end of the shaft rod (9) passes through the filter box (3).

2. The exosome extraction and collection device according to claim 1, characterized in that: The telescopic mechanism comprises a slide bar (11) which penetrates and is slidably connected to the four corners of the surface of the frame plate (5), a movable plate (12) which is fixedly connected to the bottom end of the slide bar (11) and in contact with the cam (10), and a spring (13) which is sleeved on the surface of the slide bar (11), wherein the two ends of the spring (13) are respectively connected to the frame plate (5) and the movable plate (12), and the filter screen (6) is fixedly connected to the top end of the slide bar (11).

3. The exosome extraction and collection device according to claim 1, characterized in that: The power mechanism comprises a motor (14) fixedly mounted on a side of a filter box (3) through a bracket and two pulleys (15) fixedly connected to the output end of the motor (14) and one end of a shaft (9); the output end of the motor (14) passes through the filter box (3) and is connected to a half gear (8); and the two pulleys (15) are connected by a belt tensioning connection.

4. The exosome extraction and collection device according to claim 1, characterized in that: The mixing mechanism comprises a housing (16) fixedly mounted on the top of a mixing barrel (2) and connected thereto, a moving component arranged in the housing (16), a second motor (17) arranged at the output end of the moving component, and a stirring shaft (18) fixedly connected to the output end of the second motor (17).

5. The exosome extraction and collection device according to claim 4, characterized in that: The moving part is composed of a reciprocating screw (19) rotatably connected between the two sides of the inner wall of the housing (16), a guide rod (20) fixedly installed between the two sides of the inner wall of the housing (16), a slider threadedly connected to the surface of the reciprocating screw (19) and sliding with the guide rod (20), and a driving member arranged on one side of the housing (16), and the motor 2 (17) is fixedly installed at the bottom of the slider.

6. The exosome extraction and collection device according to claim 5, characterized in that: The driving member is composed of a motor three (21) fixedly mounted on a side surface of a housing (16), and an output end of the motor three (21) passes through the housing (16) and is connected to a reciprocating screw (19).

7. The exosome extraction and collection device according to claim 1, characterized in that: The conveying member is composed of a pump body (22) fixedly mounted on a side of the filter box (3); the input end of the pump body (22) is connected to the mixing cylinder (2) via a first pipeline, and the output end of the pump body (22) extends into the filter box (3) via a second pipeline.

8. The exosome extraction and collection device according to claim 1, characterized in that: A feed pipe is fixedly mounted on the top of the mixing cylinder (2), a box cover is hingedly connected to the top of the filter box (3), and a collection drawer box (23) is arranged on the inner bottom wall of the filter box (3).

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