A sterile sampling device for stem cell culture

Abstract

The utility model discloses a sterile sampling device for stem cell culture, comprising a box body, the front surface of the box body is rotatably connected with a box door through a hinge, a cylinder is fixedly installed on the top of the inner cavity of the box body, and a lifting seat is fixedly connected to the bottom of the cylinder, an extraction tube is fixedly installed in the middle of the bottom end of the lifting seat, and a hose is fixedly connected to one side of the bottom of the extraction tube; the combined use of the set extraction tube, the hose, the fixed cylinder and the sampling tube can facilitate subsequent sampling processing, and it is no longer necessary to take samples by opening the box door, and the sterile environment inside the box body can be effectively maintained to prevent bacteria from entering the inside of the box body and affecting the normal culture of subsequent stem cells. When the stem cells are drawn into the interior of the extraction tube, they are drawn into the fixed cylinder through the set hose, and then the set sampling tube is used for sampling processing, which is convenient, fast and more practical.

Description

Technical Field

[0001] The utility model relates to the field of aseptic sampling devices for stem cell culture, in particular to an aseptic sampling device for stem cell culture. Background Art

[0002] Stem cells are a type of cell that has unlimited or immortal self-renewal ability and can produce at least one type of highly differentiated daughter cells. Stem cell research is inseparable from stem cell culture. The qualified stem cell culture process includes promoting its division and proliferation and maintaining its undifferentiated state. Maintaining a sterile environment in stem cell culture is crucial.

[0003] For example, a sterile sampling device for stem cell culture disclosed in the utility model with authorization announcement number CN219907705U limits the positions of baffle A and baffle B by a limiting plate, so that baffle A and baffle B form a gate shape, thereby achieving an isolation effect between the disinfection chamber and the isolation chamber, and maintaining a good sterile environment for stem cell culture. Since the culture of stem cells is in a sterile environment, the current sampling and processing of stem cells requires the sampling and processing by opening the box door, but such a sampling method will cause subsequent bacteria and other impurities to enter the interior of the incubator, thereby causing the culture environment of the stem cells to be infected by bacteria, thereby affecting the subsequent culture of stem cells. Utility Model Content

[0004] In order to overcome the deficiencies of the prior art, the utility model provides a sterile sampling device for stem cell culture, which can solve the problem that the existing sterile sampling device for stem cell culture limits the positions of baffle A and baffle B by a limit plate, so that baffle A and baffle B form a gate shape, thereby achieving an isolation effect between the disinfection chamber and the isolation chamber, and maintaining a good sterile environment for stem cell culture. Since the culture of stem cells is in a sterile environment, the current sampling and processing of stem cells requires the sampling and processing by opening the box door, but such a sampling method will cause subsequent bacteria and other impurities to enter the interior of the incubator, thereby causing the culture environment of the stem cells to be infected by bacteria, thereby affecting the subsequent technical problem of stem cell culture.

[0005] In order to solve the above technical problems, the utility model provides the following technical solutions: a sterile sampling device for stem cell culture, comprising a box body, the front surface of the box body is rotatably connected to a box door by a hinge, a cylinder is fixedly installed on the top of the inner cavity of the box body, and a lifting seat is fixedly connected to the bottom of the cylinder, an extraction tube is fixedly installed in the middle of the bottom end of the lifting seat, and a hose is fixedly connected to one side of the bottom of the extraction tube, the other end of the hose is connected to a fixed cylinder, and the inner cavity of the fixed cylinder is provided with an extraction structure, an extraction cavity is opened on the inner side of the fixed cylinder, and a sampling tube is provided in the inner cavity of the extraction cavity.

[0006] As a preferred technical solution of the utility model, the extraction structure includes an extraction chamber, a piston, a pull rod and a first sealing ring. The extraction chamber is opened in the inner cavity of the fixed cylinder, and a piston is arranged on the inner side of the extraction chamber. A pull rod is fixedly installed in the middle part of the top end of the piston, and a first sealing ring is sleeved on the outer surface of the pull rod.

[0007] As a preferred technical solution of the utility model, a through hole is opened on the bottom outer surface of the sampling tube, a top cover is fixedly installed on the top of the sampling tube, and a second sealing ring is arranged on the outer side of the bottom of the top cover.

[0008] As a preferred technical solution of the utility model, an ultraviolet disinfection lamp is arranged on the inner side wall of the box, and a culture dish is arranged on the bottom of the inner cavity of the box.

[0009] As a preferred technical solution of the utility model, the piston is slidably connected to the extraction tube through the pull rod, and the pull rod and the piston are vertically distributed.

[0010] As a preferred technical solution of the utility model, the through holes are equidistantly distributed along the center point of the sampling tube, and the through holes and the sampling tube form an integrated structure.

[0011] Compared with the prior art, the utility model can achieve the following beneficial effects:

[0012] 1. The combined use of the extraction tube, the hose, the fixed cylinder and the sampling tube facilitates the subsequent sampling process. It is no longer necessary to open the box door for sampling. The sterile environment inside the box can be effectively maintained to prevent bacteria from entering the inside of the box and affecting the normal culture of subsequent stem cells. When the stem cells are drawn into the extraction tube, they are drawn into the fixed cylinder through the hose, and then the sampling tube is used for sampling. It is convenient, fast and more practical.

[0013] 2. By combining the provided cylinder, lifting seat and sealing ring, the position height of the extraction tube can be adjusted according to actual use requirements, so that the subsequent sampling and processing of the cultured stem cells can be facilitated. When the cylinder is working, it will longitudinally push the lifting seat fixedly connected to its bottom, and the longitudinal movement of the lifting seat will drive the extraction tube to move longitudinally, so that the subsequent sampling and processing can be facilitated better. The provided sealing ring can improve the sealing of the entire device itself. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a schematic diagram of the structure of the aseptic sampling device for stem cell culture of the utility model;

[0015] Figure 2This is a schematic diagram of the internal structure of the box of the utility model sterile sampling device for stem cell culture;

[0016] Figure 3 This is a schematic diagram of the cross-sectional structure of the box of the utility model aseptic sampling device for stem cell culture;

[0017] Figure 4 This is a schematic diagram of the cross-sectional structure of the extraction tube of the utility model aseptic sampling device for stem cell culture;

[0018] Among them: 1. Box body; 2. Box door; 3. Cylinder; 4. Lifting seat; 5. Extraction tube; 6. Hose; 7. Fixed cylinder; 8. Extraction chamber; 9. Piston; 10. Pull rod; 11. First sealing ring; 12. Extraction chamber; 13. Sampling tube; 14. Through hole; 15. Top cover; 16. Second sealing ring; 17. Ultraviolet disinfection lamp; 18. Culture dish. DETAILED DESCRIPTION

[0019] In order to make the technical means, creative features, objectives and effects of the present invention easy to understand, the present invention is further described below in conjunction with specific embodiments, but the following embodiments are only preferred embodiments of the present invention, not all. Based on the embodiments in the implementation mode, other embodiments obtained by those skilled in the art without creative work are all within the protection scope of the present invention.

[0020] Example

[0021] Please refer to the figure, Figure 2 and Figure 3 As shown, the utility model provides a sterile sampling device for stem cell culture, comprising a box body 1, a box door 2 is rotatably connected to the front surface of the box body 1 through a hinge, a cylinder 3 is fixedly installed at the top of the inner cavity of the box body 1, and a lifting seat 4 is fixedly connected to the bottom of the cylinder 3, an extraction tube 5 is fixedly installed at the middle of the bottom end of the lifting seat 4, and a hose 6 is fixedly connected to one side of the bottom of the extraction tube 5, and the other end of the hose 6 is connected to a fixed cylinder 7, and the inner cavity of the fixed cylinder 7 is provided with an extraction structure, an extraction cavity 12 is opened on the inner side of the fixed cylinder 7, and a sampling tube 13 is provided in the inner cavity of the extraction cavity 12;

[0022] When in use, the interior of the box 1 is firstly disinfected and sterilized by using the ultraviolet disinfection lamp 17 provided on the inner wall of the box 1, and then the stem cells to be cultured are placed in the inner cavity of the culture dish 18 for culture. When sampling, the extraction tube 5 and the sampling tube 13 provided can be used to perform rapid sampling.

[0023] As a further implementation of this embodiment, Figure 1 , Figure 2 , Figure 3 and Figure 4As shown, a cylinder 3 is fixedly installed on the top of the inner cavity of the box body 1, and a lifting seat 4 is fixedly connected to the bottom of the cylinder 3, an extraction tube 5 is fixedly installed in the middle of the bottom end of the lifting seat 4, and a hose 6 is fixedly connected to one side of the bottom of the extraction tube 5, and the two ends of the hose 6 are respectively connected to the inner cavity of the extraction tube 5 and the inner cavity of the fixed cylinder 7, and the other end of the hose 6 is connected to the fixed cylinder 7, and the inner cavity of the fixed cylinder 7 is provided with an extraction structure, and through grooves, an extraction chamber 8, a piston 9, a pull rod 10 and a first sealing ring 11 are provided on both sides of the bottom of the fixed cylinder 7. The extraction chamber 8 is opened in the inner cavity of the fixed cylinder 7, and a piston 9 is provided on the inner side of the extraction chamber 8. The piston 9 is slidably connected to the extraction tube 5 through the pull rod 10, and the pull rod 10 is connected to the movable The plug 9 is distributed vertically, a pull rod 10 is fixedly installed at the middle of the top of the piston 9, and a first sealing ring 11 is sleeved on the outer surface of the pull rod 10, an extraction structure is arranged in the inner cavity of the extraction tube 5, an extraction chamber 12 is opened on the inner side of the fixed cylinder 7, and a sampling tube 13 is arranged in the inner cavity of the extraction chamber 12, a through hole 14 is opened on the outer surface of the bottom of the sampling tube 13, the through holes 14 are equidistantly distributed along the center point of the sampling tube 13, and the through holes 14 and the sampling tube 13 form an integrated structure, a top cover 15 is fixedly installed at the top of the sampling tube 13, and a second sealing ring 16 is arranged on the outer side of the bottom of the top cover 15, an ultraviolet disinfection lamp 17 is arranged on the inner side wall of the box body 1, and a culture dish 18 is arranged at the bottom of the inner cavity of the box body 1;

[0024] When sampling is required, the cylinder 3 works to push the lifting seat 4 fixedly connected to its bottom longitudinally, and then the lifting seat 4 moves longitudinally, driving the extraction tube 5 set in the middle of its bottom end to move longitudinally, and then the pull rod 10 is pulled, and then the pull rod 10 drives the piston 9 fixedly installed at the bottom to move longitudinally. Since the outer surface of the piston 9 fits the inner wall of the extraction tube 5, when the piston 9 moves longitudinally, the cells cultured inside the culture dish 18 can be extracted under the action of pressure, and then the extracted stem cells are input into the bottom of the inner cavity of the fixed tube 7 through the hose 6, and then the sampling tube 13 fixedly installed at the bottom is driven to move longitudinally through the top cover 15, so that it is input into the inner cavity of the fixed tube 7. The second sealing ring 16 can improve the sealing performance of the joint between the two to prevent dust and other bacteria from entering the inner cavity of the fixed cylinder 7. When the sampling tube 13 is completely input into the inner cavity of the fixed cylinder 7, the bottom of the sampling tube 13 contacts the bottom of the inner cavity of the fixed cylinder 7. Since the stem cell culture fluid drawn into the fixed cylinder 7 accumulates at the bottom of the inner cavity of the fixed cylinder 7, the stem cell culture fluid can be input into the bottom of the inner cavity of the sampling tube 13 in combination with the multiple through holes 14 opened on the surface of the sampling tube 13, thereby realizing the sampling and processing of stem cells, which is more convenient, faster and more practical, and can effectively prevent bacteria from being input into the inner cavity of the box body 1 during sampling, affecting the subsequent stem cell culture;

[0025] Specific working principle:

[0026] First, the interior of the box 1 is fully disinfected and sterilized by using the ultraviolet disinfection lamp 17, and then the stem cells that need to be cultured are placed in the inner cavity of the culture dish 18 for culture. When sampling, the cylinder 3 works to push the lifting seat 4 longitudinally, and then the lifting seat 4 moves longitudinally to drive the extraction tube 5 to move longitudinally, and then the pull rod 10 is pulled, and then the pull rod 10 drives the piston 9 fixedly installed at the bottom to move longitudinally. Since the outer surface of the piston 9 fits the inner wall of the extraction tube 5, when the piston 9 moves longitudinally, the cells cultured in the culture dish 18 can be extracted under the action of pressure, and then the extracted stem cells are input into the bottom of the inner cavity of the fixed cylinder 7 through the hose 6, and then the sampling tube 1 fixedly installed at the bottom is driven by the top cover 15. 3 then moves longitudinally to be input into the inner cavity of the fixed cylinder 7, and the second sealing ring 16 can be used to improve the sealing performance of the clamping point between the two to prevent dust and other bacteria from entering the inner cavity of the fixed cylinder 7. When the sampling tube 13 is completely input into the inner cavity of the fixed cylinder 7, the bottom of the sampling tube 13 contacts the bottom of the inner cavity of the fixed cylinder 7. Since the stem cell culture fluid drawn into the fixed cylinder 7 accumulates at the bottom of the inner cavity of the fixed cylinder 7, the stem cell culture fluid can be input into the bottom of the inner cavity of the sampling tube 13 in combination with the multiple through holes 14 opened on the surface of the sampling tube 13, thereby realizing the sampling and processing of stem cells, which is more convenient, faster and more practical, and can effectively prevent bacteria from being input into the inner cavity of the box body 1 during sampling to affect the subsequent stem cell culture.

[0027] The above shows and describes the basic principle, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments. The above embodiments and descriptions are only preferred examples of the utility model and are not used to limit the utility model. Without departing from the spirit and scope of the utility model, the utility model may have various changes and improvements, which fall within the scope of the utility model to be protected. The scope of protection of the utility model is defined by the attached claims and their equivalents.

Claims

1. A sterile sampling device for stem cell culture, comprising a housing (1), characterized in that: The front surface of the box body (1) is rotatably connected to a box door (2) via a hinge, a cylinder (3) is fixedly installed at the top of the inner cavity of the box body (1), and a lifting seat (4) is fixedly connected to the bottom of the cylinder (3), an extraction tube (5) is fixedly installed in the middle of the bottom end of the lifting seat (4), and a hose (6) is fixedly connected to one side of the bottom of the extraction tube (5), and the other end of the hose (6) is connected to a fixed cylinder (7), and the inner cavity of the fixed cylinder (7) is provided with an extraction structure, and an extraction chamber (12) is opened on the inner side of the fixed cylinder (7), and a sampling tube (13) is provided in the inner cavity of the extraction chamber (12).

2. A sterile sampling device for stem cell culture according to claim 1, characterized in that: The extraction structure comprises an extraction chamber (8), a piston (9), a pull rod (10) and a first sealing ring (11); the extraction chamber (8) is opened in the inner chamber of the fixed cylinder (7), and a piston (9) is arranged on the inner side of the extraction chamber (8); a pull rod (10) is fixedly installed in the middle of the top end of the piston (9), and the outer surface of the pull rod (10) is sleeved with a first sealing ring (11).

3. A sterile sampling device for stem cell culture according to claim 1, characterized in that: A through hole (14) is provided on the outer surface of the bottom of the sampling tube (13), a top cover (15) is fixedly mounted on the top of the sampling tube (13), and a second sealing ring (16) is provided on the outer side of the bottom of the top cover (15).

4. A sterile sampling device for stem cell culture according to claim 1, characterized in that: An ultraviolet disinfection lamp (17) is arranged on the inner side wall of the box (1), and a culture dish (18) is arranged at the bottom of the inner cavity of the box (1).

5. The aseptic sampling device for stem cell culture according to claim 2, characterized in that: The piston (9) is slidably connected to the extraction pipe (5) via the pull rod (10), and the pull rod (10) and the piston (9) are vertically distributed.

6. The aseptic sampling device for stem cell culture according to claim 3, characterized in that: The through holes (14) are distributed at equal distances along the center point of the sampling tube (13), and the through holes (14) and the sampling tube (13) form an integrated structure.

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