A contamination-proof PCR plate

Abstract

The utility model relates to the technical field of PCR plates, and in particular to an anti-pollution PCR plate, comprising a fixed base, a PCR plate body, and a plate cover. The plate cover is provided with a plurality of sealing components for sealing the PCR plate body, and the sealing components comprise a mounting post arranged at the bottom of the plate cover and a silicone sealing layer arranged on the mounting post. The hard mounting post of the sealing component is wrapped with a soft silicone sealing layer, which can facilitate the insertion of a detection tube in the PCR plate body while effectively ensuring the sealing performance. At the same time, each sealing component corresponds to a detection hole in the PCR plate body one by one, and no mis-plugging will occur even if it is opened and closed multiple times, thereby effectively avoiding cross-contamination of detection samples in the PCR plate body.

Description

Technical field:

[0001] The utility model relates to the technical field of PCR plates, in particular to an anti-pollution PCR plate. Background technology:

[0002] At present, in the chemical and pharmaceutical industries, it is often necessary to use high-throughput deep-well culture plates (such as 96-well plates, 384-well plates, etc.). When using deep-well culture plates to culture certain fungi, it is sometimes necessary to replace the culture medium for induction, or replace the buffer. When culturing certain bacteria or yeasts, it is sometimes necessary to remove the culture medium from each deep well in subsequent operations, or after the lysis treatment, centrifugation is required to remove cell debris. In short, in many cases, the substances in the deep-well plate need to be separated by solid and liquid. In order to avoid liquid or sample contamination in different reaction tubes, the conventional operation is to use a sealing film or a silicone cover to seal directly on the PCR plate. However, the sealing film is directly coated with a glue layer on the PET layer, and the glue layer is continuous and cannot avoid the opening of the detection hole, which is easy to cause sample cross-contamination. When using a silicone cover for sealing, due to the soft structure of the silicone cover body, its protrusion is not easy to accurately plug into the detection hole, and the sealing effect is poor. The sealing film or silicone cover installed directly on the PCR plate for sealing is easy to fall off, and there is also a risk of sample exposure infection, which cannot meet the needs of long-distance transportation and is inconvenient to use. Utility model content:

[0003] The purpose of the utility model is to provide an anti-contamination PCR plate in view of the deficiencies in the prior art, which has a good sealing effect and can effectively ensure that the samples in each detection hole of the PCR plate can be sealed under different usage situations, thereby reducing the risk of cross contamination.

[0004] In order to achieve the above-mentioned purpose, the technical solution adopted by the utility model is: an anti-pollution PCR plate, including a fixed base, a PCR plate body, and a plate cover, the fixed base is provided with a PCR plate fixing groove for the PCR plate body to be placed, and the PCR plate fixing groove is provided with a plurality of groups of PCR tube mounting grooves for the tube body in the PCR plate body to be placed; the plate cover is provided with a plurality of sealing components for sealing the PCR plate body, and the sealing components include a mounting column arranged at the bottom of the plate cover and a silicone sealing layer arranged on the mounting column.

[0005] A further improvement to the above solution is that a cut corner is provided in the PCR plate fixing groove.

[0006] A further improvement to the above solution is that the plate cover and the fixed base are detachably connected together.

[0007] A further improvement to the above scheme is that snap connectors are provided in the side walls on both sides of the plate cover, snap connection grooves matching with the snap connectors are provided on the outer walls on both sides of the fixed base, and the snap connectors are snap-connected with the snap connection grooves in cooperation with each other.

[0008] A further improvement to the above solution is that handle grooves are provided on both sides of the plate cover.

[0009] A further improvement to the above solution is that a circular hole edge is provided outside the periphery of the opening of the tube body in the PCR plate body.

[0010] A further improvement to the above scheme is that a plurality of positioning posts are provided at the bottom of the plate cover, a plurality of positioning holes corresponding to the positioning posts are provided on the PCR plate body, and the positioning posts are inserted into the positioning holes.

[0011] The utility model has the beneficial effects of: an anti-pollution PCR plate, comprising a fixed base, a PCR plate body, and a plate cover, wherein the fixed base is provided with a PCR plate fixing groove for the PCR plate body to be placed therein, and the PCR plate fixing groove is provided with a plurality of groups of PCR tube installation grooves for the tube bodies in the PCR plate body to be placed therein; the plate cover is provided with a plurality of sealing components for sealing the PCR plate body, and the sealing components include a mounting column arranged at the bottom of the plate cover, and a silicone sealing layer arranged on the mounting column;

[0012] A plate cover that can be buckled with a fixed base is provided, and a sealing component for sealing is provided on the plate cover. A hard mounting column of the sealing component is wrapped with a soft silicone sealing layer. The structure is hard inside and soft outside. While effectively ensuring the sealing, the detection tube in the PCR plate body can be easily inserted, which is convenient for rapid positioning during sealing. The silicone sealing layer is provided to effectively improve the anti-vibration buffering performance, prevent the PCB board from being bumped during transportation, and meet the needs of long-distance transportation. The silicone sealing layer is provided to prevent the detection liquid from spilling during transportation and causing cross-contamination. At the same time, each sealing component corresponds to the detection hole in the PCR plate body one by one. Even if it is opened and closed multiple times, it will not be accidentally plugged, which effectively avoids cross-contamination of detection samples in the PCR plate body. Description of the drawings:

[0013] Figure 1 It is a schematic diagram of the structure of the utility model.

[0014] Figure 2 It is a side sectional view of the utility model.

[0015] Figure 3 for Figure 2 Enlarged view of point A in the middle.

[0016] Figure 4It is a structural schematic diagram of the PCR plate of the utility model.

[0017] Explanation of the reference numerals: fixed base 1, PCR plate fixing groove 11, cut corner 12, PCR tube mounting groove 13, PCR plate body 2, circular hole edge 21, positioning hole 22, snap connection groove 23, handle groove 24, plate cover 3, snap connector 31, positioning column 32, sealing assembly 33, mounting column 331, silicone sealing layer 332. Specific implementation method:

[0018] The utility model will be further described below in conjunction with the accompanying drawings. Figure 1-4 As shown, the utility model provides an anti-pollution PCR plate, including a fixed base 1, a PCR plate body 2, and a plate cover 3. The fixed base 1 is provided with a PCR plate fixing groove 11 for the PCR plate body 2 to be placed therein, and the PCR plate fixing groove 11 is provided with a plurality of groups of PCR tube mounting grooves 13 for the tube bodies in the PCR plate body 2 to be placed therein; the plate cover 3 is provided with a plurality of sealing components 33 for sealing the PCR plate body 2, and the sealing components 33 include a mounting column 331 arranged at the bottom of the plate cover 3, a silicone sealing layer 332 arranged on the mounting column 331, and a silicone sealing layer 333 arranged between the silicone sealing layer 332 and the plate cover 3; by providing a plate cover 3 that can be buckled with the fixed base 1, the plate cover 3 is provided with a useful The sealing component 33 is sealed, and the hard mounting column 331 of the sealing component 33 is wrapped with a soft silicone sealing layer 332. It adopts a hard-inside and soft-outside structure, which can effectively ensure the sealing while being easy to insert the detection tube in the PCR plate body 2, and is convenient for rapid positioning during sealing; by setting the silicone sealing layer 332, the anti-vibration buffering performance is effectively improved, and the PCB board is prevented from being bumped during transportation, meeting the needs of long-distance transportation; at the same time, the silicone sealing layer 332 can detect liquid that is spilled during transportation and causes cross-contamination. At the same time, each sealing component 33 corresponds to the detection hole in the PCR plate body 2 one by one, and will not be accidentally plugged even if it is opened and closed multiple times, effectively avoiding cross-contamination of detection samples in the PCR plate body 2.

[0019] A cut corner 12 is provided in the PCR plate fixing groove 11, and the position of the cut corner 12 can be used to judge whether the position of the PCR plate body 2 is consistent before and after insertion, thereby preventing operational errors caused by confusing the direction of the PCR plate body 2 after adding liquid and pipetting, and avoiding contamination of samples.

[0020] The plate cover 3 is detachably connected to the fixed base 1. In the present embodiment, snap connectors 31 are arranged in the side walls on both sides of the plate cover 3, and snap connector grooves 23 matching with the snap connectors 31 are arranged on the outer walls on both sides of the fixed base 1. The snap connectors 31 are snap-connected with the snap connector grooves 23, which facilitates the user to separate the cover plate and the fixed base 1. Of course, other detachable modes can also be provided, and the present invention is not limited thereto.

[0021] Handle grooves 24 are provided on both sides of the plate cover 3 to facilitate operators to open the cover.

[0022] A circular hole edge 21 is arranged outside the opening periphery of the inner tube body of the PCR plate body 2. The raised circular hole edge 21 can effectively prevent the detection liquid brought out when the cover is opened from flowing from the hole mouth to other detection holes, thereby reducing the risk of exposure.

[0023] A plurality of positioning posts 32 are provided at the bottom of the plate cover 3, and a plurality of positioning holes 22 corresponding to the positioning posts 32 are provided on the PCR plate body 2. The positioning posts 32 are inserted into the positioning holes 22, and the positioning posts 32 correspond to the positioning holes 22 one by one. When covering each cover plate, the position can be effectively aligned, the positioning can be quickly performed, and the sealing is convenient. At the same time, it is ensured that each sample detection hole is sealed with the same sealing component 33 before opening, so as to effectively prevent cross contamination.

[0024] Working principle:

[0025] When in use, the user puts the PCR plate body 2 into the PCR plate fixing groove 11 in the fixing base 1. The plate cover 3 is provided with a plurality of sealing components 33 for sealing the PCR plate body 2. The sealing components 33 include mounting posts 331 arranged at the bottom of the plate cover 3 and a silicone sealing layer 332 arranged on the mounting posts 331. By providing a plate cover 3 that can be buckled with the fixing base 1, the plate cover 3 is provided with a sealing component 33 for sealing, and the hard mounting posts 331 of the sealing component 33 are wrapped with a soft silicone sealing layer 332, adopting a hard inside and soft outside structure. The structure can facilitate the insertion of the detection tube in the PCR plate body 2 while effectively ensuring the sealing, and facilitates rapid positioning when sealing; by setting the silicone sealing layer 332, the anti-vibration buffering performance is effectively improved, and the PCB board is prevented from being bumped during transportation, meeting the needs of long-distance transportation; at the same time, the silicone sealing layer prevents the detection liquid from spilling during transportation and causing cross contamination. At the same time, each sealing component 33 corresponds to the detection hole in the PCR plate body 2 one by one, and there will be no accidental plugging even if it is opened and closed multiple times, which effectively avoids cross contamination of detection samples in the PCR plate body 2.

[0026] Of course, the above are only preferred implementations of the present utility model, so all equivalent changes or modifications made according to the structure, features and principles of the scope of the present utility model patent application are included in the scope of the present utility model patent application.

Claims

1. A contamination-resistant PCR plate, characterized in that: The invention comprises a fixed base (1), a PCR plate body (2), and a plate cover (3); the fixed base (1) is provided with a PCR plate fixing groove (11) for the PCR plate body (2) to be placed therein; the PCR plate fixing groove (11) is provided with a plurality of groups of PCR tube installation grooves (13) for the tube bodies in the PCR plate body (2) to be placed therein; the plate cover (3) is provided with a plurality of sealing components (33) for sealing the PCR plate body (2); the sealing components (33) comprise a mounting column (331) arranged at the bottom of the plate cover (3) and a silicone sealing layer (332) arranged on the mounting column (331).

2. The anti-contamination PCR plate according to claim 1, characterized in that: A cut corner (12) is arranged in the PCR plate fixing groove (11).

3. The anti-contamination PCR plate according to claim 1, characterized in that: The plate cover (3) is detachably connected to the fixed base (1).

4. The anti-contamination PCR plate according to claim 3, characterized in that: The side walls of the plate cover (3) are provided with snap-fit ​​connectors (31), and the outer walls of the fixed base (1) are provided with snap-fit ​​connector grooves (23) that match the snap-fit ​​connectors (31). The snap-fit ​​connectors (31) are snap-fitted with the snap-fit ​​connector grooves (23).

5. The anti-contamination PCR plate according to claim 1, characterized in that: Handle grooves (24) are provided on both sides of the plate cover (3).

6. The anti-contamination PCR plate according to claim 1, characterized in that: A circular hole edge (21) is arranged outside the peripheral edge of the opening of the inner tube body of the PCR plate body (2).

7. The anti-contamination PCR plate according to claim 1, characterized in that: The bottom of the plate cover (3) is provided with a plurality of positioning posts (32), and the PCR plate body (2) is provided with a plurality of positioning holes (22) corresponding one-to-one to the positioning posts (32), and the positioning posts (32) are inserted into the positioning holes (22).

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