Cell suspension preparation device

Abstract

The utility model relates to the field of suspension preparation, in particular to a cell suspension preparation device which comprises a shell and a washing cavity connected with the interior of the shell, a kidney-shaped hole is formed in the side face of the washing cavity and penetrates through the side face of the shell, and a sealing cover is connected to the outer side of the shell and used for replacing a biomass sample from the side face of the shell. The sealing cover is rotatably connected with a rotary knob, one end of the rotary knob is connected with a barrier plate, a sample groove is formed in the side surface of the sealing cover, the inner side wall of the sample groove is connected with an arc-shaped tray, and the arc-shaped tray is connected with a plurality of fixing needles for fixing a biomass sample. And through reciprocating water flow scouring, cells on the surface of the biomass sample can be separated in the scouring process, so that the sampling effect of the device is realized.

Description

Technical Field

[0001] The utility model relates to the technical field of suspension preparation, in particular to a cell suspension preparation device. Background Art

[0002] Flow cytometry can quickly measure cell volume, internal structure, DNA, antigens and other properties. In clinical and experimental research, mouse spleen is often used as test material for immune cell surface antigen staining, intracellular staining, cell proliferation detection, etc. These experiments require the preparation of spleen single cell suspension, which can be used for flow cytometer detection after staining and washing.

[0003] The Chinese patent with publication number CN220132210U discloses a single cell suspension preparation device, which relates to the field of single cell suspension preparation equipment, and includes a preparation body, the interior of the preparation body is hollow, one end of the preparation body is provided with a movable port, and the other end is provided with a liquid collection port, the opening area of ​​the movable port is larger than the opening area of ​​the liquid collection port; a piston push rod, which passes through the movable port, and the piston push rod is slidably inserted in the preparation body, and the sliding direction is parallel to the central axis of the preparation body; a filter cup, which is coaxially arranged in the preparation body, and the filter cup is located on the moving path of pushing and pulling the front end of the piston push rod, and the filter cup is open on the side facing the piston push rod, and the filter cup is used to hold solid tissue. The utility model has the effect of reducing resource waste, facilitating operation, and improving the accuracy of experimental results when preparing single cell suspensions.

[0004] However, the above-mentioned disclosed scheme has the following shortcomings: the above-mentioned device collects samples by squeezing the filter cup with a push rod, so that the cells in the sample are dispersed in the squeezed state, which is convenient for extracting single-cell samples. However, the above-mentioned device adopts the squeezing method for sampling. For soft biomass raw materials, the above-mentioned device will cause the cells in the sample to rupture during the sampling process, thereby causing the cell fluid inside the cell to mix into the physiological saline when preparing the single-cell suspension, thereby reducing the purity of the sample. Utility Model Content

[0005] The purpose of the utility model is to provide a cell suspension preparation device to solve the problems raised by the above background technology.

[0006] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: the device adopts a flushing method to perform live sampling on the surface of the biomass sample. Through reciprocating water flushing, the cells on the surface of the biomass sample can be detached during the flushing process, thereby achieving the sampling effect of the device. Compared with traditional devices, the device avoids the extrusion link, thereby reducing the damage to the biomass sample while reducing the risk of cell rupture.

[0007] The utility model provides a cell suspension preparation device, comprising a shell and a flushing cavity connected thereto, a waist-shaped hole is arranged on the side of the flushing cavity, the waist-shaped hole runs through the side of the shell, and a sealing cover is connected to the outside of the shell for replacing a biomass sample from the side of the shell;

[0008] The sealing cover is rotatably connected with a rotating knob, one end of which is connected with a blocking plate, a sample groove is arranged on the side of the sealing cover, an arc-shaped tray is connected with the inner wall of the sample groove, and a plurality of fixing pins are connected to the arc-shaped tray for fixing the biomass sample.

[0009] Preferably, the bottom of the flushing cavity is connected to a liquid outlet hopper, the bottom of the liquid outlet hopper is connected to a control valve, and a control knob is provided on the side of the control valve.

[0010] Preferably, the top of the flushing cavity is connected to a funnel connector, the other end of the funnel connector is connected to a hydraulic cavity, the inner wall of the hydraulic cavity is slidably connected to a hydraulic plate, the top of the hydraulic plate is connected to a sliding column, and the top of the sliding column is connected to a pressing plate.

[0011] Preferably, an observation window is provided on the side of the shell, the observation window faces the direction of the hydraulic cavity, and a convex lens is provided on the observation window.

[0012] Preferably, a waterproof component is provided at the edge of the sealing cover, and the waterproof component is in contact and extrusion with the inner wall of the waist-shaped hole on the side of the shell.

[0013] Preferably, the length of the blocking plate is greater than the width of the waist-shaped hole of the shell, and an arc-shaped abutment member is provided on the side of the blocking plate, and the arc-shaped abutment member is in contact with the inner side wall of the flushing cavity.

[0014] Preferably, a sealing ring is provided at the edge of the hydraulic plate, the sealing ring is in contact and extrusion with the side of the inner wall of the hydraulic cavity, the diameter of the pressing plate is larger than the diameter of the hydraulic cavity, and the top of the hydraulic cavity is open.

[0015] The above technical solution of the utility model has the following beneficial technical effects:

[0016] The device adopts a flushing method to perform live sampling on the surface of the biomass sample. Through reciprocating water flushing, the cells on the surface of the biomass sample can be detached during the flushing process, thereby achieving the sampling effect of the device. Compared with the traditional device, the device avoids the squeezing link, thereby reducing the damage to the biomass sample while reducing the risk of cell rupture. The sliding structure of the hydraulic plate can increase the water flushing speed of the device, so that the device can extract free single-cell samples, and the device can be discharged by controlling the valve. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1It is a front view of an embodiment of the cell suspension preparation device of the utility model;

[0018] Figure 2 This is a schematic diagram of the structure of the observation window in the embodiment of the utility model;

[0019] Figure 3 This is a structural schematic diagram of the funnel connector in the embodiment of the utility model;

[0020] Figure 4 for Figure 3 A cross-sectional view of

[0021] Figure 5 for Figure 4 A magnified view of the structure at center;

[0022] Figure numerals: 1. Shell; 2. Liquid outlet bucket; 3. Control valve; 4. Sealing cover; 5. Turning knob; 6. Observation window; 7. Sliding column; 8. Pressing plate; 9. Hydraulic chamber; 10. Funnel connector; 11. Flushing chamber; 12. Blocking plate; 13. Sample groove; 14. Arc tray; 15. Fixing needle; 16. Hydraulic plate. DETAILED DESCRIPTION

[0023] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other. The present application will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0024] In order to enable those skilled in the art to better understand the solution of the present application, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present application.

[0025] Example: See Figure 1-5 The utility model provides a technical solution: a cell suspension preparation device, comprising a shell 1 and a flushing cavity 11 connected thereto, a waist-shaped hole is provided on the side of the flushing cavity 11, and the waist-shaped hole penetrates the side of the shell 1, and a sealing cover 4 is connected to the outside of the shell 1 for replacing the biomass sample from the side of the shell 1, and an observation window 6 is provided on the side of the shell 1, and the observation window 6 faces the direction of the hydraulic cavity 9;

[0026] Furthermore, a liquid outlet bucket 2 is connected to the bottom of the flushing chamber 11, a control valve 3 is connected to the bottom of the liquid outlet bucket 2, and a control knob is provided on the side of the control valve 3. The structure of the flushing chamber 11 and the sealing cover 4 can ensure the sealing of the internal space of the device, avoiding the influence of the external environment on the interior of the device during the sampling process, thereby ensuring the purity of the sample and avoiding contamination of the suspension.

[0027] The sealing cover 4 is rotatably connected to a rotating knob 5, one end of which is connected to a blocking plate 12, a sample groove 13 is provided on the side of the sealing cover 4, an arc-shaped tray 14 is connected to the inner wall of the sample groove 13, and a plurality of fixing needles 15 are connected to the arc-shaped tray 14, and a barb is provided on the top of the fixing needle 15 for fixing the biomass sample. The barb structure can prevent the biomass sample from falling when the flushing cavity 11 is inverted;

[0028] Furthermore, a waterproof member is provided at the edge of the sealing cover 4, and the waterproof member contacts and squeezes the inner wall of the waist-shaped hole on the side of the housing 1;

[0029] Specifically, the length of the blocking plate 12 is greater than the width of the waist-shaped hole of the shell 1, and an arc-shaped abutment member is provided on the side of the blocking plate 12, which contacts the inner wall of the flushing cavity 11. By turning the knob 5 to control the blocking plate 12, it can be ensured that the sealing cover 4 can be stably sealed, effectively preventing the device from leaking liquid and other problems.

[0030] The top of the flushing cavity 11 is connected to a funnel connector 10, the other end of the funnel connector 10 is connected to a hydraulic cavity 9, the inner wall of the hydraulic cavity 9 is slidably connected to a hydraulic plate 16, the top of the hydraulic plate 16 is connected to a sliding column 7, and the top of the sliding column 7 is connected to a pressing plate 8;

[0031] Furthermore, a sealing ring is provided at the edge of the hydraulic plate 16, and the sealing ring contacts and squeezes the inner wall side of the hydraulic cavity 9. The diameter of the pressing plate 8 is larger than the diameter of the hydraulic cavity 9, and the top of the hydraulic cavity 9 is open. The structure of the hydraulic plate 16 can improve the efficiency of physiological saline passing through the funnel connector 10, thereby improving the sampling efficiency of the device. At the same time, the pressing plate 8 can form a high-pressure water flow inside the funnel connector 10, which is convenient for flushing and shedding cells on the surface of the biomass.

[0032] This device can effectively improve the sampling efficiency of intact cells. Compared with traditional devices, this device uses a reciprocating water flushing method to effectively improve the sampling efficiency of intact cells.

[0033] When the device is in working state, the blocking plate 12 can be placed in a vertical state by unlocking and rotating the knob 5, so that the sealing cover 4 can be easily removed. At this time, the biomass raw material is inserted on the top of the fixing needle 15, thereby achieving the sample fixing effect of the device.

[0034] After the sample is fixed, the overall sealing effect of the device is ensured by placing the sealing cover 4 inside the waist-shaped hole on the side of the shell 1. After the sealing cover 4 is extended into the flushing cavity 11, rotating the knob 5 can make the blocking plate 12 in a horizontal state, thereby achieving a locking sealing effect on the sealing cover 4.

[0035] After the sealing cover 4 is installed, the bottom control valve 3 is opened, and the physiological saline can be sucked from the control valve 3 by pulling the pressing plate 8 upward, so that the physiological saline remains in the flushing cavity 11 .

[0036] At this time, the device is inverted, and the control valve 3 is directed upward. At this time, pushing the pressing plate 8 upward can discharge excess air. After the excess air is discharged, the pressing plate 8 is pulled downward to allow the physiological saline to enter the hydraulic cavity 9.

[0037] When the pressing plate 8 is pulled downward, external air enters the flushing cavity 11 from the control valve 3 , thereby ensuring that the physiological saline can flow into the hydraulic cavity 9 .

[0038] When the pressing plate 8 is pulled to the bottom, the plate 8 is pressed upward, so that the saline in the hydraulic cavity 9 can be sprayed upward through the funnel connector 10. Under the pressure of the pressing plate 8 pushing upward, the saline forms a high-pressure water flow when passing through the funnel connector 10, thereby achieving the effect of flushing the surface of the biomass sample with water.

[0039] By repeatedly pulling the pressing plate 8 up and down several times, the biomass sample can be repeatedly flushed with saline. When the saline enters the hydraulic cavity 9 again, it can be observed through the observation window 6 whether there are free single-cell samples in the saline, so as to judge whether the current sampling process is successful.

[0040] After observing the presence of free single-cell samples in the physiological saline, the device is placed upright so that the control valve 3 faces directly downward. A culture dish is placed under the control valve 3. Since a portion of the physiological saline is in the hydraulic cavity 9, the physiological saline passes through the funnel connector 10 at a slower speed. At this time, by squeezing the pressing plate 8 downward, the physiological saline inside the device can flow out quickly, thereby improving the use efficiency of the device.

[0041] Those skilled in the art should understand that the discussion of any of the above embodiments is merely illustrative and is not intended to imply that the scope of the present invention (including the claims) is limited to these examples. Under the concept of the present invention, the technical features in the above embodiments or different embodiments may be combined, the steps may be implemented in any order, and there are many other variations of the different aspects of the present invention as described above, which are not provided in detail for the sake of simplicity.

[0042] The utility model is intended to cover all such substitutions, modifications and variations that fall within the broad scope of the appended claims. Therefore, any omissions, modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the utility model should be included in the scope of protection of the utility model.

Claims

1. A cell suspension preparation device, comprising a housing (1) and a flushing chamber (11) connected thereto, characterized in that: A waist-shaped hole is provided on the side of the flushing cavity (11), and the waist-shaped hole penetrates the side of the shell (1). A sealing cover (4) is connected to the outside of the shell (1) and is used to replace the biomass sample from the side of the shell (1); The sealing cover (4) is rotatably connected to a rotating knob (5), one end of the rotating knob (5) is connected to a blocking plate (12), a sample groove (13) is provided on the side of the sealing cover (4), an arc-shaped tray (14) is connected to the inner side wall of the sample groove (13), and the arc-shaped tray (14) is connected to a plurality of fixing pins (15) for fixing the biomass sample.

2. The cell suspension preparation device according to claim 1, characterized in that: The bottom of the flushing cavity (11) is connected to a liquid outlet hopper (2), the bottom of the liquid outlet hopper (2) is connected to a control valve (3), and a control knob is provided on the side of the control valve (3).

3. The cell suspension preparation device according to claim 1, characterized in that: The top of the flushing cavity (11) is connected to a funnel connector (10), the other end of the funnel connector (10) is connected to a hydraulic cavity (9), the inner wall of the hydraulic cavity (9) is slidably connected to a hydraulic plate (16), the top of the hydraulic plate (16) is connected to a sliding column (7), and the top of the sliding column (7) is connected to a pressing plate (8).

4. The cell suspension preparation device according to claim 3, characterized in that: An observation window (6) is provided on the side of the housing (1), the observation window (6) faces the direction of the hydraulic chamber (9), and a convex lens is provided on the observation window (6).

5. The cell suspension preparation device according to claim 1, characterized in that: A waterproof component is provided at the edge of the sealing cover (4), and the waterproof component is in contact and extrusion with the inner wall of the waist-shaped hole on the side of the shell (1).

6. The cell suspension preparation device according to claim 1, characterized in that: The length of the blocking plate (12) is greater than the width of the waist-shaped hole of the shell (1), and an arc-shaped abutment piece is provided on the side of the blocking plate (12), and the arc-shaped abutment piece contacts the inner side wall of the flushing cavity (11).

7. The cell suspension preparation device according to claim 3, characterized in that: A sealing ring is provided at the edge of the hydraulic plate (16), and the sealing ring contacts and squeezes the inner wall side of the hydraulic cavity (9). The diameter of the pressing plate (8) is larger than the diameter of the hydraulic cavity (9), and the top of the hydraulic cavity (9) is open.

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