Exosome centrifugal device

Abstract

The utility model relates to the technical field of exosome extraction and separation, in particular to an exosome centrifugal device which comprises a plurality of sets of centrifugal tube limiting mechanisms, each set of centrifugal tube limiting mechanism comprises a machine frame, a plurality of evenly-distributed connecting supports are fixedly installed at the bottom end of each machine frame, and the connecting supports are connected with the centrifugal tube limiting mechanisms. The bottom ends of the connecting supports are fixedly mounted at the inner bottom end of the mounting frame, a plurality of evenly-distributed guide grooves are formed in the top ends of the racks, and guide blocks are slidably mounted in the guide grooves. The device has the beneficial effects that a guide block can move along a guide groove under the cooperation of a control air cylinder, an air cylinder matched connecting plate, a connecting rod and an auxiliary plate, so that a limiting plate can abut against the periphery of a centrifugal tube, and at the moment, the centrifugal tube can be limited; according to the centrifugal device, the situation of relative displacement of centrifugal tubes with different sizes in the centrifugal process due to infirm fixation is avoided, meanwhile, the adjustable limiting plate can adapt to the centrifugal tubes with different sizes and specifications, and the practicability of the centrifugal device is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of exosome extraction and separation, and specifically to an exosome centrifugal device. Background Art

[0002] Stem cell exosomes are subcellular structures and functional factors paracrine to stem cells. After various biological information and bioactive substances of stem cell exosomes enter the receptor cells or target cells, they play and realize the cell communication and material exchange between stem cells and receptor cells or target cells, thereby ultimately exerting various biological effects and functions of stem cell exosomes, as well as regulating the proliferation, differentiation, migration, apoptosis, etc. of receptor cells or target cells.

[0003] In the prior art, methods for extracting exosomes include centrifugation, polymerization sedimentation, and ultrafiltration. However, the centrifuge tube sleeve in the centrifuge device can only adapt to matching centrifuge tubes. When centrifuge tubes of other specifications and sizes are used, the centrifuge tubes may not be firmly fixed or may be relatively displaced, affecting the normal use of the centrifuge tubes. Utility Model Content

[0004] The purpose of the utility model is to provide an exosome centrifugal device to solve the problems raised in the above background technology.

[0005] To achieve the above objectives, the utility model provides the following technical solutions: an exosome centrifugal device, comprising:

[0006] A workbench, wherein a mounting frame is rotatably mounted at the middle of the top of the workbench;

[0007] A plurality of groups of centrifuge tube limiting mechanisms, each group of the centrifuge tube limiting mechanisms comprises a frame, a plurality of evenly distributed connecting brackets are fixedly installed at the bottom end of the frame, the bottom ends of the connecting brackets are fixedly installed at the bottom end of the mounting frame, a plurality of evenly distributed guide grooves are opened at the top of the frame, guide blocks are slidably installed in the guide grooves, limiting plates are fixedly installed at the tops of the guide blocks, connecting seats are fixedly installed at the bottom ends of the guide blocks, connecting rods are rotatably installed at the lower parts of the connecting seats, connecting plates are rotatably installed at the lower parts of the connecting rods in the same group, mounting seats are fixedly installed at the middle part of the top end of the frame, a plurality of evenly distributed auxiliary plates are fixedly installed at the top end of the frame, and the lower parts of the auxiliary plates are rotatably installed at the middle part of the connecting rods on the same side;

[0008] The driving mechanism is arranged at the lower part of the workbench.

[0009] Preferably, a cylinder is fixedly installed in the lower part of the frame, and the driving ends of the cylinders are respectively fixedly installed in the middle of the bottom end of the connecting plate on the same side.

[0010] Preferably, installation grooves are provided in the middle of both sides of the guide block, and a plurality of evenly distributed balls are respectively embedded and installed in the installation grooves.

[0011] Preferably, a spring is fixedly mounted at the middle of one end of the guide block away from the mounting seat, and one end of the spring away from the guide block is respectively fixedly mounted at the middle of the side wall of the guide groove on the same side.

[0012] Preferably, the driving mechanism comprises a rotating seat, which is rotatably mounted in the middle of the workbench and connected to the mounting frame.

[0013] Preferably, a motor mounting plate is fixedly installed on one side of the bottom end of the workbench, a stepper motor is fixedly installed in the middle of the motor mounting plate, a driving wheel is fixedly installed on the driving end of the stepper motor, the driving wheel drives the driven wheel to rotate through a synchronous belt, and the driven wheel is fixedly installed on the lower part of the rotating seat.

[0014] Compared with the prior art, the beneficial effects of the utility model are:

[0015] The exosome centrifuge device proposed in the present invention is provided with a centrifuge tube limiting mechanism, which can move the guide block along the guide groove by controlling the cylinder, and the cylinder cooperates with the connecting plate, the connecting rod and the auxiliary plate, so that the limiting plate can abut against the outer periphery of the centrifuge tube. At this time, the centrifuge tube can be limited to avoid relative displacement of centrifuge tubes of different sizes due to loose fixation during the centrifugation process. At the same time, the adjustable limiting plate can adapt to centrifuge tubes of different sizes, which is conducive to enhancing the practicality of the centrifuge device. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic diagram of the main three-dimensional structure of the utility model;

[0017] Figure 2 This is a schematic diagram of the local structure of the driving mechanism of the utility model;

[0018] Figure 3 This is a schematic diagram of the internal structure of the mounting frame of the utility model;

[0019] Figure 4 This is a partial structural diagram of the centrifuge tube limiting mechanism of the utility model;

[0020] Figure 5 It is a schematic diagram of the local structure of the guide block of the utility model.

[0021] In the figure: 1. workbench; 2. mounting frame; 3. driving mechanism; 301. motor mounting plate; 302. stepping motor; 303. driving wheel; 304. synchronous belt; 305. driven wheel; 306. rotating seat; 4. centrifuge tube limiting mechanism; 401. frame; 402. limiting plate; 403. mounting seat; 404. guide block; 405. connecting seat; 406. connecting rod; 407. auxiliary plate; 408. cylinder; 409. connecting bracket; 4010. guide groove; 4011. ball; 4012. mounting groove; 4013. spring; 4014. connecting plate. DETAILED DESCRIPTION

[0022] In order to make the purpose and technical solution of the utility model clearly and completely described, and the advantages more clearly understood, the embodiments of the utility model are further described in detail in conjunction with the accompanying drawings. It should be understood that the specific embodiments described here are part of the embodiments of the utility model, not all of the embodiments, and are only used to explain the embodiments of the utility model, and are not used to limit the embodiments of the utility model. All other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0023] See also Figures 1 to 5 The utility model provides a technical solution: an exosome centrifugal device, comprising: a workbench 1, a mounting frame 2 is rotatably installed at the middle of the top of the workbench 1, a plurality of centrifuge tube limiting mechanisms 4, each group of centrifuge tube limiting mechanisms 4 includes a frame 401, a plurality of evenly distributed connecting brackets 409 are fixedly installed at the bottom end of the frame 401, the bottom ends of the connecting brackets 409 are fixedly installed at the bottom end of the mounting frame 2, a plurality of evenly distributed guide grooves 4010 are opened at the top of the frame 401, guide blocks 404 are slidably installed in the guide grooves 4010, a limiting plate 402 is fixedly installed at the top of the guide block 404, a connecting seat 405 is fixedly installed at the bottom end of the guide block 404, a connecting rod 406 is rotatably installed at the lower part of the connecting seat 405, and a connecting plate 406 is rotatably installed at the lower part of the same group of connecting rods 406. 14. A mounting seat 403 is fixedly installed at the middle of the top of the frame 401. A plurality of evenly distributed auxiliary plates 407 are fixedly installed at the top of the frame 401, and the lower parts of the auxiliary plates 407 are rotatably installed at the middle of the connecting rod 406 on the same side. A mounting groove 4012 is provided on the upper part of the mounting frame 2. At this time, the staff can place the centrifuge tube in the mounting groove 4012. At this time, by controlling the cylinder 408 in the frame 401, the cylinder 408 can push the connecting plate 4014 to move. At this time, the connecting rod 406 can move the guide block 404 along the guide groove 4010 with the cooperation of the auxiliary plate 407, and then the limiting plate 402 can abut against the outer periphery of the centrifuge tube. At this time, the centrifuge tube can be limited, avoiding the relative displacement of centrifuge tubes of different sizes due to loose fixation during the centrifugation process.

[0024] A cylinder 408 is fixedly installed in the lower part of the frame 401, and the driving end of the cylinder 408 is fixedly installed in the middle of the bottom end of the connecting plate 4014 on the same side. A mounting groove 4012 is opened in the middle of both sides of the guide block 404, and a plurality of evenly distributed balls 4011 are respectively embedded and installed in the mounting groove 4012. A spring 4013 is fixedly installed in the middle of the end of the guide block 404 away from the mounting seat 403. The end of the spring 4013 away from the guide block 404 is fixedly installed in the middle of the side wall of the guide groove 4010 on the same side. A ball 4011 is provided inside 04 to reduce the sliding friction of the guide block 404 in the guide groove 4010, thereby improving the smoothness of the guide block 404 during movement, and a spring 4013 is provided inside the guide block 404 and the guide groove 4010. The spring 4013 uses its own elasticity to limit the range of movement of the guide block 404. When the guide block 404 reaches the limit position, the spring 4013 will generate a certain elastic force to offset the inertia force of the moving parts, thereby stopping the guide block 404 at the limit position.

[0025] The driving mechanism 3 is arranged at the lower part of the workbench 1. The driving mechanism 3 includes a rotating seat 306. The rotating seat 306 is rotatably installed in the middle of the workbench 1 and is connected to the mounting frame 2. A motor mounting plate 301 is fixedly installed on one side of the bottom end of the workbench 1. A stepper motor 302 is fixedly installed in the middle of the motor mounting plate 301. A driving wheel 303 is fixedly installed at the driving end of the stepper motor 302. The driving wheel 303 drives the driven wheel 305 to rotate through a synchronous belt 304. The driven wheel 305 is fixedly installed at the lower part of the rotating seat 306. By controlling the stepper motor 302, the stepper motor 302 can drive the driving wheel 303 to rotate. Since the driving wheel 303 is connected to the driven wheel 305 through the synchronous belt 304, the driven wheel 305 cooperates with the rotating seat 306 to drive the mounting frame 2 to rotate on the workbench 1, so that the stem cell solution in the centrifuge tube can be centrifuged, so as to extract the relevant exosomes.

[0026] During actual use, the staff places the centrifuge tube containing the stem cell solution in the slot in the mounting frame 2. At this time, by controlling the cylinder 408, the cylinder 408 can push the connecting plate 4014 to move. The connecting plate 4014 cooperates with the connecting rod 406 and the auxiliary plate 407 to realize the movement of the guide block 404 in the guide slot 4010. At this time, the limit plate 402 will approach the centrifuge tube until the centrifuge tube is limited and fixed. At the same time, an arc-shaped rubber pad is provided on the surface of the limit plate 402 to prevent the limit plate 402 from making rigid contact with the centrifuge tube and causing damage to the centrifuge tube. Then, the stepper motor 302 cooperates with the driving wheel 303, the driven wheel 305, the synchronous belt 304 and the rotating seat 306 to realize the rotation of the mounting frame 2, thereby completing the centrifugal operation on the cell solution in the centrifuge tube.

[0027] Although the above describes the illustrative specific implementation methods of the present application so that technicians in this technical field can understand the present application, the present application is not limited to the scope of the specific implementation methods. For ordinary technicians in this technical field, as long as various changes are within the spirit and scope of the present application defined and determined by the attached claims, all application creations using the concept of the present application are protected.

Claims

1. An exosome centrifugal device, characterized in that: include: A workbench (1), wherein a mounting frame (2) is rotatably mounted at the middle of the top end of the workbench (1); A plurality of centrifuge tube limiting mechanisms (4), each of which comprises a frame (401), a plurality of evenly distributed connecting brackets (409) are fixedly mounted at the bottom of the frame (401), the bottom of the connecting brackets (409) are fixedly mounted at the bottom of the mounting frame (2), a plurality of evenly distributed guide grooves (4010) are opened at the top of the frame (401), a guide block (404) is slidably mounted in the guide groove (4010), and a limiting block (404) is fixedly mounted at the top of the guide block (404). The guide block (404) is fixedly mounted with a connecting seat (405) at the bottom end, and a connecting rod (406) is rotatably mounted at the bottom of the connecting seat (405). The connecting rods (406) of the same group are rotatably mounted with connecting plates (4014) at the bottom. A mounting seat (403) is fixedly mounted at the middle of the top end of the frame (401). A plurality of evenly distributed auxiliary plates (407) are fixedly mounted at the top end of the frame (401), and the lower parts of the auxiliary plates (407) are rotatably mounted at the middle of the connecting rods (406) on the same side. A driving mechanism (3), wherein the driving mechanism (3) is arranged at the lower part of the workbench (1).

2. An exosome centrifugal device according to claim 1, characterized in that: A cylinder (408) is fixedly installed in the lower part of the frame (401), and the driving end of the cylinder (408) is fixedly installed in the middle of the bottom end of the connecting plate (4014) on the same side.

3. The exosome centrifugal device according to claim 1, characterized in that: The guide block (404) is provided with mounting grooves (4012) in the middle of both sides, and a plurality of evenly distributed balls (4011) are respectively embedded and installed in the mounting grooves (4012).

4. The exosome centrifugal device according to claim 1, characterized in that: A spring (4013) is fixedly mounted at the middle of one end of the guide block (404) away from the mounting seat (403), and one end of the spring (4013) away from the guide block (404) is fixedly mounted at the middle of the side wall of the guide groove (4010) on the same side.

5. The exosome centrifugal device according to claim 1, characterized in that: The driving mechanism (3) comprises a rotating seat (306), the rotating seat (306) is rotatably mounted in the middle of the workbench (1), and the rotating seat (306) is connected to the mounting frame (2).

6. The exosome centrifugal device according to claim 1, characterized in that: A motor mounting plate (301) is fixedly mounted on one side of the bottom end of the workbench (1), a stepper motor (302) is fixedly mounted in the middle of the motor mounting plate (301), a driving wheel (303) is fixedly mounted on the driving end of the stepper motor (302), the driving wheel (303) drives a driven wheel (305) to rotate via a synchronous belt (304), and the driven wheel (305) is fixedly mounted on the lower part of a rotating seat (306).

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