Full-automatic nucleic acid extractor

Abstract

The utility model discloses a full-automatic nucleic acid extractor which comprises a bottom plate module, the bottom plate module comprises a bottom plate and ground feet, the bottom plate provides a supporting and fixing platform for other modules, and the ground feet are distributed on one side of the bottom plate in a rectangular shape; the magnetic suction uniform mixing mechanism is fixedly connected to the other surface of the bottom plate module, plays a role in uniform mixing and magnetic suction in an experiment, and comprises a motor device, a vertical plate, a screw rod, a first fixing block, a second fixing block, a linear guide rail and a screw rod end seat; and the turntable rotating mechanism is fixedly connected to the position, close to the magnetic suction mixing mechanism, of the bottom plate module and comprises a fixed support, a turntable motor, a turntable, a high-precision speed reducer and a turntable connecting piece. The full-automatic nucleic acid extractor solves the problems that an existing product control system is complex, high in cost and low in operation precision after being used for a long time, and has the advantages of being low in cost, high in control precision, capable of operating for a long time with high precision and low in after-sale difficulty.

Description

Technical Field

[0001] The utility model relates to the field of nucleic acid extraction, in particular to a full-automatic nucleic acid extraction instrument. Background Art

[0002] Nucleic acid extractors are instruments that use matching nucleic acid extraction reagents to automatically complete sample nucleic acid extraction. In almost every laboratory, separation and purification work related to biological molecules is very important and indispensable. The fully automatic nucleic acid extractor is automated in the nucleic acid extraction experiment, which greatly saves manpower and brings great convenience to the experimenters. The operation and switching of different component reagents during the extraction process is also automated. Usually, a three-dimensional mechanical motion mechanism is used to realize the magnetic rod reaching the area of ​​different component reagents, or a motor drives a synchronous pulley to drive a turntable to realize a circular motion mechanical operation mechanism to switch different component reagents to the magnetic rod position.

[0003] The fully automatic nucleic acid extractors commonly found on the market use three-dimensional mechanical motion mechanisms to enable the magnetic rod to reach the areas of different reagent components. This technology often requires a large operating space, resulting in a large instrument size, occupying a large space in the laboratory, and the electrical control system is relatively complex and costly, but the control accuracy is not high, and the coordinate accuracy of the instrument needs to be calibrated after a period of use. There are also some circular motion mechanical structures used on the market that use motors to drive synchronous pulleys to drive the turntable to achieve operation switching of different component reagents, but the synchronous belts will wear and loosen during use, and the operating accuracy will decrease after long-term use, requiring regular replacement of the synchronous belts or tensioning of the synchronous belts. The above technologies used on the market cannot achieve high-precision operation, and require after-sales calibration and maintenance in the later stage, which increases the difficulty of the work of after-sales personnel. Utility Model Content

[0004] The main purpose of the utility model is to provide a fully automatic nucleic acid extraction instrument, which overcomes the above technical problems.

[0005] In order to achieve the above purpose, the utility model proposes the following technical solutions:

[0006] Fully automatic nucleic acid extraction instrument, including:

[0007] The base module includes: a base and a foot. The base provides a supporting and fixing platform for other modules. The foot is distributed in a rectangular shape on one side of the base.

[0008] A magnetic mixing mechanism, which is fixedly connected to the other side of the base plate module and plays a role of mixing and magnetic attraction in the experiment, including: a motor device, a vertical plate, a screw, a first fixed block, a second fixed block, a linear guide rail, and a screw end seat, wherein: the vertical plate is fixedly connected to the base plate, a motor device is connected below the vertical plate, the motor device is close to the connection between the vertical plate and the base plate, the top of the motor device is connected to one end of the two screws, the other ends of the two screws are rotatably connected to the screw end seats, and two sliding blocks that can slide in the length direction of the linear guide rail are provided on the linear guide rail, which are fixed to the same side of the vertical plate as the screw end seat; one end of the first fixed block is connected to one of the screws through a thread, and the other end is connected to one of the sliding blocks of the linear guide rail; one end of the second fixed block is connected to one of the screws through a thread, and the other end is connected to another sliding block of the linear guide rail;

[0009] The turntable rotating mechanism is fixedly connected to the position of the base plate module near the magnetic mixing mechanism, and includes: a fixed support, a turntable motor, a turntable, a high-precision reducer, and a turntable connecting piece, wherein: the fixed support is fixedly connected to the base plate, the high-precision reducer is fixedly connected to the end of the fixed support away from the base plate, the turntable motor is connected to a side of the high-precision reducer close to the base plate, the side of the high-precision reducer away from the base plate is connected to a turntable connecting piece, and a turntable is connected above the turntable connecting piece.

[0010] Furthermore, the fully automatic nucleic acid extractor also includes a temperature module, which is fixedly connected to the turntable 05 and is used to control the temperature required during the experiment.

[0011] Furthermore, the fully automatic nucleic acid extractor also includes a housing module for protecting components and increasing aesthetics.

[0012] Furthermore, the turntable rotating mechanism also includes a turntable cover, which is fixed at the middle position of the turntable and is used to cover the assembly gap and fixing screws between the turntable connecting piece and the turntable.

[0013] Furthermore, the turntable rotation mechanism also includes: a limit plate and a reset photoelectric device, both of which are connected to a high-precision reducer, and the limit plate and the reset photoelectric device can complete the zero-point reset function of the turntable.

[0014] Furthermore, the turntable rotating mechanism also includes: a limit column and a limit block, the limit column is fixedly connected to the turntable connecting piece, the limit block is fixedly connected to the fixed support, and the limit column and the limit block jointly play a role of mechanical limiting.

[0015] Furthermore, the motor device includes: a magnetic rod motor, a magnetic rod sleeve motor and a motor fixing base, the motor fixing base is fixedly connected to the vertical plate, the magnetic rod motor and the magnetic rod sleeve motor are both fixedly connected to the side of the motor fixing base close to the bottom plate, and the rotating shafts pass through the motor fixing base respectively, and a screw rod is connected to the top of each of the magnetic rod motor and the magnetic rod sleeve motor.

[0016] Furthermore, the motor device also includes a coupling, and the two couplings are respectively connected to the rotating shafts of the magnetic rod motor and the magnetic rod sleeve motor, and the other ends of the two couplings are respectively connected to one end of the two screw rods.

[0017] Furthermore, a fan module is arranged on the turntable, the fan module and the temperature module are arranged symmetrically, and the fan module is used for cooling.

[0018] Furthermore, the magnetic attraction mixing mechanism also includes: a magnetic rod group fixing plate, a magnetic rod group and a magnetic rod sleeve clamp, the magnetic rod group is fixed on the magnetic rod group fixing plate, the magnetic rod group fixing plate is connected to the second fixing block, the magnetic rod sleeve clamp is connected to the first fixing block, and the magnetic rod sleeve is connected to the magnetic rod sleeve clamp;

[0019] A deep-hole plate is arranged on the turntable, and the deep-hole plate, the magnetic rod sleeve and the magnetic rod group cooperate to complete the magnetic attraction and mixing functions.

[0020] The fully automatic nucleic acid extractor of the utility model solves the problems of complex control systems, high costs, and decreased operating accuracy after long-term use of existing products. It has the advantages of low cost, high control accuracy, long-term high-precision operation, and low after-sales difficulty. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The drawings constituting part of the present application are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation on the present invention. In the drawings:

[0022] Figure 1 The figure shows the appearance and structure diagram of the fully automatic nucleic acid extractor of the present utility model.

[0023] Figure 2 The figure shows the internal structure of the fully automatic nucleic acid extractor of the utility model.

[0024] Figure 3 The utility model shows a schematic diagram of the turntable rotating mechanism structure of the fully automatic nucleic acid extractor.

[0025] The above drawings include the following reference numerals:

[0026] 01. Base plate; 02. Anchor; 03. Fixed support; 04. Turntable motor; 05. Turntable; 06. Temperature module; 07. Fan module; 08. Vertical plate; 09. Magnetic rod motor; 10. Magnetic rod sleeve motor; 11. Motor fixing seat; 12. Coupling; 13. Screw; 14. First fixing block; 15. Second fixing block; 16. Linear guide; 17. Screw end seat; 18. Magnetic rod group fixing plate; 19. Magnetic rod group; 20. Magnetic rod sleeve clamp; 21. Magnetic rod sleeve; 22. Deep hole plate; 23. High-precision reducer; 24. Turntable connector; 25. Turntable cover; 26. Limit plate; 27. Reset photoelectric; 28. Limit column; 29. ​​Limit block. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. The following description of at least one exemplary embodiment is actually only illustrative and is by no means a limitation on the utility model and its application or use. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the utility model.

[0028] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.

[0029] Unless otherwise specifically stated, the relative arrangement, numerical expressions and numerical values ​​of the parts and steps described in these embodiments do not limit the scope of the utility model. At the same time, it should be understood that, for ease of description, the sizes of the various parts shown in the accompanying drawings are not drawn according to the actual proportional relationship. The technology, methods and equipment known to ordinary technicians in the relevant field may not be discussed in detail, but in appropriate cases, the technology, methods and equipment should be regarded as a part of the authorization specification. In all examples shown and discussed here, any specific value should be interpreted as merely exemplary, rather than as a limitation. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters represent similar items in the following drawings, so once a certain item is defined in one drawing, it does not need to be further discussed in subsequent drawings.

[0030] Reference below Figures 1 to 3, the utility model is further described as follows:

[0031] Fully automatic nucleic acid extraction instrument, including:

[0032] The base module includes: a base plate 01 and a base foot 02. The base plate 01 provides a support and fixing platform for other modules. The base foot 02 is distributed in a rectangular shape on one side of the base plate 01.

[0033] Magnetic mixing mechanism, the magnetic mixing mechanism is fixedly connected to the other side of the base plate module, and plays a role of mixing and magnetic attraction in the experiment, including: a motor device, a vertical plate 08, a screw 13, a first fixed block 14, a second fixed block 15, a linear guide rail 16, and a screw end seat 17, wherein: the vertical plate 08 is fixedly connected to the base plate 01, and a motor device is connected to the bottom of the vertical plate, the motor device is close to the connection between the vertical plate 08 and the base plate 01, the top of the motor device is connected to one end of the two screw rods 13, and the other ends of the two screw rods 13 are rotatably connected to the screw end seat 17, and two sliding blocks that can slide in the length direction of the linear guide rail 16 are provided on the linear guide rail 16, and are fixed on the same side of the vertical plate 08 as the screw end seat 17; one end of the first fixed block 14 is connected to one of the screw rods 13 through a thread, and the other end is connected to one of the sliding blocks of the linear guide rail 16 One end of the second fixed block 15 is connected to one of the lead screws 13 through a thread, and the other end is connected to another sliding block of the linear guide 16. The magnetic suction mixing mechanism also includes: a magnetic rod group fixing plate 18, a magnetic rod group 19 and a magnetic rod sleeve clamp 20. The magnetic rod group 19 is fixed on the magnetic rod group fixing plate 18, the magnetic rod group fixing plate 18 is connected to the second fixed block 15, the magnetic rod sleeve clamp 20 is connected to the first fixed block 14, and the magnetic rod sleeve 21 is connected to the magnetic rod sleeve clamp 20; the motor device includes: a magnetic rod motor 09, a magnetic rod sleeve motor 10 and a motor fixing seat 11, the motor fixing seat 11 is fixedly connected to the vertical plate 08, the magnetic rod motor 09 and the magnetic rod sleeve motor 10 are both fixedly connected to one side of the motor fixing seat 11 close to the bottom plate 01, and the rotating shafts pass through the motor fixing seat 11 respectively, and a lead screw 13 is connected to the top of the magnetic rod motor 09 and the magnetic rod sleeve motor 10.

[0034] A deep hole plate 22 is provided on the turntable 05, and the deep hole plate 22, the magnetic rod sleeve 21 and the magnetic rod group 19 cooperate to complete the magnetic attraction and mixing functions. The above structure forms a linear running mechanism of the magnetic rod group and the magnetic rod sleeve in the Z-axis direction, realizing the mixing and magnetic attraction functions in the experiment.

[0035] In an optional embodiment, the motor device further includes a coupling 12 , and two couplings 12 are respectively connected to the rotating shafts of the magnetic rod motor 09 and the magnetic rod sleeve motor 10 , and the other ends of the two couplings 12 are respectively connected to one end of two screw rods 13 .

[0036] The turntable rotating mechanism is fixedly connected to the position of the base plate module near the magnetic mixing mechanism, including: a fixed support 03, a turntable motor 04, a turntable 05, a high-precision reducer 23, and a turntable connecting piece 24, wherein: the fixed support 03 is fixedly connected to the base plate 01, the high-precision reducer 23 is fixedly connected to the end of the fixed support 03 away from the base plate 01, the turntable motor 04 is connected to a side of the high-precision reducer 23 close to the base plate 01, the side of the high-precision reducer 23 away from the base plate 01 is connected to the turntable connecting piece 24, and the turntable 05 is connected above the turntable connecting piece 24.

[0037] In this embodiment, the fully automatic nucleic acid extractor also includes a temperature module 06, which is fixedly connected to the turntable 05 and is used to control the temperature required during the experiment. A fan module 07 is provided on the turntable 05, and the fan module 07 is symmetrically arranged with the temperature module 06, and the fan module 07 is used for cooling.

[0038] The temperature module 06 is composed of a heat conducting plate that carries the deep-well plate, a heating plate, a temperature probe, a fan, etc. The heating plate provides temperature to the heat conducting plate, and the heat conducting plate then transmits the temperature to the deep-well plate. The fan module has the function of cooling the heat conducting plate, and the temperature probe controls the temperature required during the experiment.

[0039] In this embodiment, the fully automatic nucleic acid extractor further includes a housing module for protecting components and increasing aesthetics. The housing module can effectively prevent dust from forming on the internal components of the device, and can also reduce damage to the internal components when a collision occurs, thereby reducing the impact on the accuracy of the device.

[0040] In this embodiment, the turntable rotating mechanism further includes a turntable cover 25, which is fixed in the middle of the turntable 05 to cover the assembly gap and fixing screws between the turntable connector 24 and the turntable 05. The turntable cover 25 not only has an aesthetic effect, but also has a certain protective effect.

[0041] In this embodiment, the turntable rotation mechanism also includes: a limit plate 26 and a reset photoelectric 27, both of which are connected to the high-precision reducer 23, and the limit plate 26 and the reset photoelectric 27 can complete the zero point reset function of the turntable. The limit plate 26 is fixed on the rotating part of the high-precision reducer 23, and the reset photoelectric 27 is fixed on the non-rotating part of the high-precision reducer 23. The reset photoelectric 27 controls the operation of the turntable motor 04 and the high-precision reducer 23 through electrical signals, and cooperates with the limit plate 26 to achieve the zero point reset function.

[0042] In this embodiment, the turntable rotation mechanism also includes: a limit column 28 and a limit block 29. The limit column 28 is fixedly connected to the turntable connecting member 24, and the limit block 29 is fixedly connected to the fixed support 03. The limit column 28 and the limit block 29 jointly play a role of mechanical limiting. When the rotation of the turntable 05 is out of control during the test, the mechanical limiting structure can timely and effectively stop the turntable 05 from continuing to rotate, thereby reducing the adverse effects caused by the loss of control.

[0043] In the description of the present utility model, it needs to be understood that the directions or positional relationships indicated by directional words such as "front, back, up, down, left, right", "lateral, vertical, perpendicular, horizontal" and "top, bottom" are usually based on the directions or positional relationships shown in the drawings, and are only for the convenience of describing the present utility model and simplifying the description. Unless otherwise specified, these directional words do not indicate or imply that the device or element referred to must have a specific direction or be constructed and operated in a specific direction, and therefore cannot be understood as limiting the scope of protection of the present utility model; the directional words "inside and outside" refer to the inside and outside relative to the contours of each component itself.

[0044] For ease of description, spatially relative terms such as "above", "above", "on the upper surface of", "above", etc. may be used here to describe the spatial positional relationship between a device or feature and other devices or features as shown in the figure. It should be understood that spatially relative terms are intended to include different orientations of the device in use or operation in addition to the orientation described in the figure. For example, if the device in the accompanying drawings is inverted, the device described as "above other devices or structures" or "above other devices or structures" will be positioned as "below other devices or structures" or "below other devices or structures". Thus, the exemplary term "above" can include both "above" and "below". The device can also be positioned in other different ways (rotated 90 degrees or in other orientations), and the spatially relative descriptions used here are interpreted accordingly.

[0045] In addition, it should be noted that the use of terms such as "first" and "second" to limit components is only for the convenience of distinguishing the corresponding components. If not otherwise stated, the above terms have no special meaning and therefore cannot be understood as limiting the scope of protection of the utility model.

[0046] The above description is only the preferred embodiment of the utility model, and is not intended to limit the utility model. For those skilled in the art, the utility model can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the utility model shall be included in the protection scope of the utility model.

Claims

1. A fully automatic nucleic acid extractor, characterized in that: include: The base plate module comprises: a base plate (01) and a foot (02), wherein the base plate (01) provides a support and fixing platform for other modules, and the foot (02) is distributed in a rectangular shape on one side of the base plate (01); A magnetic mixing mechanism, which is fixedly connected to the other side of the base plate module and plays a role of mixing and magnetic attraction in the experiment, comprises: a motor device, a vertical plate (08), a screw rod (13), a first fixed block (14), a second fixed block (15), a linear guide rail (16), and a screw rod end seat (17), wherein: the vertical plate (08) is fixedly connected to the base plate (01), a motor device is connected below the vertical plate, the motor device is close to the connection between the vertical plate (08) and the base plate (01), the top of the motor device is connected to one end of the two screw rods (13), and the two screw rods (13) are connected to the motor device. The other end of the first fixed block (14) is rotatably connected to the screw rod end seat (17); the linear guide rail (16) is provided with two sliding blocks that can slide in the length direction of the linear guide rail (16) and are fixed on the same side of the vertical plate (08) as the screw rod end seat (17); one end of the first fixed block (14) is connected to one of the screw rods (13) through a thread, and the other end is connected to one of the sliding blocks of the linear guide rail (16); one end of the second fixed block (15) is connected to one of the screw rods (13) through a thread, and the other end is connected to the other sliding block of the linear guide rail (16); A turntable rotating mechanism, the turntable rotating mechanism is fixedly connected to the position of the base plate module close to the magnetic suction mixing mechanism, comprising: a fixed support (03), a turntable motor (04), a turntable (05), a high-precision reducer (23), and a turntable connecting member (24), wherein: the fixed support (03) is fixedly connected to the base plate (01), the high-precision reducer (23) is fixedly connected to an end of the fixed support (03) away from the base plate (01), the turntable motor (04) is connected to a side of the high-precision reducer (23) close to the base plate (01), the turntable connecting member (24) is connected to a side of the high-precision reducer (23) away from the base plate (01), and the turntable (05) is connected above the turntable connecting member (24).

2. The fully automatic nucleic acid extractor according to claim 1, characterized in that: The fully automatic nucleic acid extractor also includes a temperature module, which is fixedly connected to the turntable (05) and is used to control the temperature required during the experiment.

3. The fully automatic nucleic acid extractor according to claim 1, characterized in that: The fully automatic nucleic acid extractor also includes a housing module for protecting components and improving aesthetics.

4. The fully automatic nucleic acid extractor according to claim 1, characterized in that: The turntable rotating mechanism also includes a turntable cover (25), which is fixed at the middle position of the turntable (05) and is used to cover the assembly gap and fixing screws between the turntable connecting member (24) and the turntable (05).

5. The fully automatic nucleic acid extractor according to claim 1, characterized in that: The turntable rotating mechanism further comprises: a limit plate (26) and a reset photoelectric device (27). The limit plate (26) and the reset photoelectric device (27) are both connected to the high-precision reducer (23). The limit plate (26) and the reset photoelectric device (27) can complete the zero point reset function of the turntable.

6. The fully automatic nucleic acid extractor according to claim 1, characterized in that: The turntable rotating mechanism further comprises: a limit column (28) and a limit block (29); the limit column (28) is fixedly connected to the turntable connecting member (24); the limit block (29) is fixedly connected to the fixed support (03); the limit column (28) and the limit block (29) jointly play a role of mechanical limit.

7. The fully automatic nucleic acid extractor according to claim 1, characterized in that: The motor device comprises: a magnetic rod motor (09), a magnetic rod sleeve motor (10) and a motor fixing seat (11); the motor fixing seat (11) is fixedly connected to the vertical plate (08); the magnetic rod motor (09) and the magnetic rod sleeve motor (10) are both fixedly connected to a side of the motor fixing seat (11) close to the bottom plate (01); and the rotating shafts respectively pass through the motor fixing seat (11); and the tops of the magnetic rod motor (09) and the magnetic rod sleeve motor (10) are each connected to a screw rod (13).

8. The fully automatic nucleic acid extractor according to claim 7, characterized in that: The motor device further comprises a coupling (12), wherein the two couplings (12) are respectively connected to the rotating shafts of the magnetic rod motor (09) and the magnetic rod sleeve motor (10), and the other ends of the two couplings (12) are respectively connected to one end of the two lead screws (13).

9. The fully automatic nucleic acid extractor according to claim 1, characterized in that: The rotating disk (05) is provided with a fan module (07), the fan module (07) and the temperature module (06) are symmetrically arranged, and the fan module (07) is used for cooling.

10. The fully automatic nucleic acid extractor according to claim 1, characterized in that: The magnetic attraction mixing mechanism further comprises: a magnetic rod group fixing plate (18), a magnetic rod group (19) and a magnetic rod sleeve clamp (20), wherein the magnetic rod group (19) is fixed on the magnetic rod group fixing plate (18), the magnetic rod group fixing plate (18) is connected to the second fixing block (15), the magnetic rod sleeve clamp (20) is connected to the first fixing block (14), and the magnetic rod sleeve (21) is connected to the magnetic rod sleeve clamp (20); The turntable (05) is provided with a deep-hole plate (22), and the deep-hole plate (22), the magnetic rod sleeve (21) and the magnetic rod group (19) cooperate to complete the magnetic attraction and mixing functions.

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