Improved magnetic bead cleaning and liquid absorbing device in PCR (Polymerase Chain Reaction) experiment

Abstract

The utility model discloses an improved device for magnetic bead cleaning and liquid suction in a PCR (Polymerase Chain Reaction) experiment, which comprises a suction head withdrawing sleeve and sample tubes, and further comprises a magnetic frame, a plurality of suction head withdrawing sleeves and a plurality of sample tubes, a plurality of suction head withdrawing sleeves are assembled on the sample bracket, the sample bracket is assembled on the magnetic frame in a sliding manner, and the sample bracket is positioned above the sample tube; the electric pipette is driven to move along the vertical direction; and the inserting part is assembled at the port of the electric pipette and is assembled with the suction head withdrawing sleeve in an inserting manner along the vertical direction. According to the improved magnetic bead cleaning and liquid suction device in the PCR experiment, waste liquid is sucked out through cooperation of the electric pipette and the multiple suction head withdrawing sleeves, then the electric pipette moves upwards so that the suction head withdrawing sleeves can be separated, the old suction head withdrawing sleeves can stay in the sample support, a new suction head withdrawing sleeve can be replaced by pulling the sample support, the suction head withdrawing sleeves do not need to be manually replaced many times, and the operation is simple and convenient. The suction efficiency is improved, and the probability of pollution caused by replacement of the gun head is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of nucleic acid extraction devices, in particular to an improved device for washing and aspirating magnetic beads in PCR experiments. Background Art

[0002] Magnetic bead extraction has become one of the most common nucleic acid extraction methods in clinical PCR experiments. During this process, it is often necessary to wash the magnetic beads multiple times with different washing solutions and repeatedly remove the waste liquid. The conventional use of a pipette to remove the waste liquid is inefficient and repeated operations are prone to contamination.

[0003] In conjunction with publication number CN103527528A, published on 2014-01-22, a siphon drainage device for use in a magnetic bead-based molecular purification device is disclosed.

[0004] In the prior art including the above patent, the reagent is discharged from the test tube by the principle of siphon, and its basic structure is composed of two parts, the first part is a sample test tube rack, and the second part is a siphon tube structure. The sample test tube and the siphon tube are interconnected, and the reagent can flow from the first part into the second part. The above technology adds fresh reagent to the test tube until the liquid level reaches the top of the siphon to cause siphoning, and then stops adding reagent. When the liquid level in the test tube is lower than the siphon inlet, the siphoning stops. At this time, the siphon device returns to the starting state and can enter the next cycle. It does not require a complex electric control device, but the absorption of waste liquid is completely achieved through the structure of the siphon tube. The range of the amount of liquid required for magnetic bead purification is large, and the lower limit of the liquid absorption is relatively trace. It is difficult to directly absorb it using a siphon tube, and it requires repeated operations many times, which is more troublesome. Utility Model Content

[0005] The utility model aims to provide an improved device for washing and aspirating magnetic beads in PCR experiments, so as to solve the above problems.

[0006] In order to achieve the above-mentioned purpose, the utility model provides the following technical solutions: an improved device for magnetic bead washing and liquid aspiration in PCR experiments, comprising a suction head cover and a sample tube, including:

[0007] A magnetic rack for assembling a plurality of sample tubes;

[0008] A sample holder, on which a plurality of suction head covers are mounted, the sample holder is slidably mounted on the magnetic holder, and the sample holder is located above the sample tube, and the suction head covers correspond to the sample tubes in sequence;

[0009] An electric pipette gun is driven to move in a vertical direction;

[0010] A plug-in portion, which is assembled on the port of the electric pipette gun and is plug-assembled with the suction withdrawal head cover in the vertical direction, and the number of the plug-in portion is one or a plurality of plug-in portions arranged linearly;

[0011] Wherein, magnetic parts for attracting magnetic beads in the sample tube are arranged on both sides of the inner wall of the magnetic frame.

[0012] Preferably, a negative pressure aspirator is further included, and the negative pressure aspirator is communicated with the electric pipette.

[0013] Preferably, it further comprises a disposable tube head bin fixedly arranged on the workbench, wherein a plurality of sample holders are mounted on the disposable tube head bin, and the sample holders are all arranged in parallel in a horizontal direction.

[0014] Preferably, the sample holder may have four holes, eight holes or twelve holes.

[0015] Preferably, a moving mechanism is provided in the disposable tube head compartment to move the sample holders one by one to a predetermined height, and the predetermined height corresponds to the assembly end of the magnetic rack.

[0016] Preferably, a driving mechanism for driving the electric pipette is provided on the workbench.

[0017] Preferably, the disposable tube head is plugged into the socket at the bottom of the sample holder, and the socket is provided with a stopper to limit the upward movement of the suction head cover.

[0018] In the above technical scheme, the utility model provides an improved device for magnetic bead washing and liquid aspiration in PCR experiments, which has the following beneficial effects: waste liquid is aspirated out by the cooperation of an electric pipette and a plurality of suction head covers, and then the electric pipette is moved upward to disengage the suction head cover and allow the old suction head cover to remain in the sample holder, and a new suction head cover is replaced by pulling out the sample holder, without the need to manually replace the suction head cover multiple times, thereby improving the suction efficiency and reducing the probability of contamination caused by replacing the gun tip. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present utility model. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0020] Figure 1 An overall three-dimensional schematic diagram provided for an embodiment of the utility model;

[0021] Figure 2 This is a schematic diagram of the positions of the magnetic rack, sample holder, and suction head cover provided in an embodiment of the utility model.

[0022] Description of reference numerals:

[0023] 1. Electric pipette; 2. Driving mechanism; 3. Tip cover; 4. Disposable pipette head; 5. Sample holder; 6. Negative pressure aspirator; 7. Disposable pipette head bin; 8. Magnetic stand; 81. Magnetic part. DETAILED DESCRIPTION

[0024] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0025] like Figure 1-2 As shown, an improved device for washing and aspirating magnetic beads in a PCR experiment includes a suction head cover 3 and a sample tube, including:

[0026] A magnetic rack 8, which is used to assemble multiple sample tubes;

[0027] A sample holder 5, on which a plurality of suction head covers 3 are mounted, the sample holder 5 is slidably mounted on the magnetic holder 8, and the sample holder 5 is located above the sample tubes, and the suction head covers 3 correspond to the sample tubes in sequence;

[0028] An electric pipette 1, which is driven to move in a vertical direction;

[0029] A plug-in portion 4, which is assembled on the port of the electric pipette gun 1 and plug-assembled with the suction withdrawal head cover 3 in the vertical direction. The number of the plug-in portion 4 is one or a plurality of plug-in portions 4 arranged linearly;

[0030] Wherein, magnetic parts 81 for attracting magnetic beads in the sample tube are arranged on both sides of the inner wall of the magnetic frame 8 .

[0031] Specifically, the electric pipette 1 sets the volume and time of each aspiration through a control program. The above-mentioned electronic components are common technical knowledge in the art and will not be described in detail here.

[0032] Furthermore, the sample holder 5 with multiple suction head covers 3 inserted is slidably assembled on the magnetic frame 8, and then the electric pipette gun 1 is driven to move downward. Since multiple plug-in parts 4 can be assembled at the end of the electric pipette gun 1, multiple or single plug-in parts 4 are driven to move downward in the vertical direction until the plug-in part 4 extends into the suction head cover 3 to be inserted and assembled with the suction head cover 3. Then, the electric pipette gun 1 continues to move downward to move the suction head cover 3 into the sample tube. At this time, the magnetic beads in the sample tube are adsorbed to both sides by the magnetic part 81, which facilitates the suction head cover 3 to perform suction in the middle. Liquid is then removed from the sample tube by sliding the electric pipette gun 1 upward. The suction withdrawal head cover 3 is then removed from the sample tube. When the suction withdrawal head cover 3 moves to the position of the sample holder 5, it is restricted by the block on the sample holder 5 and stays on the sample holder 5. Since the sample holder 5 has four holes, eight holes or twelve holes, multiple suction withdrawal head covers 3 can be replaced at one time. At this time, the plug-in portion 4 is disengaged from the suction withdrawal head cover 3, and the sample holder 5 is disassembled by sliding along the magnetic holder 8. The suction withdrawal head cover 3 that has completed the suction has been completely removed, and the next sample holder 5 is replaced. The above operation is repeated until the waste liquid in the sample tube is completely sucked out.

[0033] The sliding sample holder 5 can be slid manually, or driven by a linear motor to slide the sample holder 5, or by any other driving method known to those skilled in the art.

[0034] In the above technology, the waste liquid is sucked out by the cooperation of the electric pipette 1 and multiple suction head covers 3, and then the electric pipette 1 is moved upward to disengage the suction head cover 3, and the old suction head cover 3 is allowed to stay in the sample holder 5. The new suction head cover 3 is replaced by pulling the sample holder 5, and there is no need to manually replace the suction head cover 3 multiple times, which improves the suction efficiency and reduces the probability of contamination caused by replacing the gun tip.

[0035] As an embodiment further provided by the present invention, it also includes a negative pressure aspirator 6 , which is connected to the electric pipette gun 1 .

[0036] Specifically, the negative pressure suction device 6 can continuously absorb the waste liquid in the electric pipette 1 for a long time, so that the electric pipette 1 can be equipped with multiple suction head covers 3 to suck a large batch of disposable tube heads 4. At the same time, the negative pressure suction device 6 collects the waste liquid for subsequent processing.

[0037] As another embodiment further provided by the present invention, it also includes a disposable tube head bin 7 fixedly arranged on a workbench, and a plurality of sample holders 5 are mounted on the disposable tube head bin 7, and the sample holders 5 are all arranged in parallel in a horizontal direction.

[0038] Specifically, the disposable tube head bin 7 can be a sealed rectangular box-like structure, or an open rack-like structure, or any other structure that meets the sample storage requirements, so that the staff can easily take out the sample holder 5 that has not been aspirated.

[0039] As another embodiment further provided by the present invention, a moving mechanism is provided in the disposable tube head compartment 7 to move the sample holders 5 one by one to a predetermined height, and the predetermined height is opposite to the assembly end of the magnetic frame 8 .

[0040] Specifically, the assembly end of the magnetic rack 8 is the end for assembling the sample holder 5. The sample holder 5 in the disposable tube head compartment 7 is driven by the moving mechanism to reach a predetermined height. At this time, the sample holder 5 corresponds to the assembly end of the magnetic rack 8 and has the same height. When the sample holder 5 on the magnetic rack 8 is slid and removed, the sample holder 5 in the disposable tube head compartment 7 is extended out of the disposable tube head compartment 7 by manual sliding or linear motor driving. At this time, the sample holder 5 corresponds to and is assembled with the assembly end of the magnetic rack 8.

[0041] As another embodiment further provided by the present invention, it also includes a driving mechanism 2 for driving the electric pipette 1.

[0042] Specifically, the driving mechanism 2 is connected to the control program, and the control program is used to set the distance and dwell time of each movement, thereby regulating the moving path of the electric pipette 1. The driving mechanism 2 can use a linear motor, an electric telescopic rod, or a driving device known to technicians in this field.

[0043] The above only describes some exemplary embodiments of the present invention by way of illustration. It is undoubted that those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. An improved device for washing and aspirating magnetic beads in a PCR experiment, comprising a suction head cover (3) and a sample tube, characterized in that: include: A magnetic rack (8) for assembling a plurality of sample tubes; A sample holder (5) on which a plurality of suction head covers (3) are mounted, the sample holder (5) being slidably mounted on a magnetic holder (8), and the sample holder (5) being located above the sample tubes, and the suction head covers (3) corresponding to the sample tubes in sequence; An electric pipette (1) driven to move in a vertical direction; A plug-in portion (4), which is assembled on the port of the electric liquid transfer gun (1) and is plug-assembled with the suction withdrawal head cover (3) in a vertical direction, and the number of the plug-in portion (4) is one or a plurality of plug-in portions (4) arranged linearly; Wherein, magnetic parts (81) for attracting magnetic beads in the sample tube are arranged on both sides of the inner wall of the magnetic frame (8).

2. The improved device for washing and aspirating magnetic beads in a PCR experiment according to claim 1, characterized in that: It also includes a negative pressure suction device (6), and the negative pressure suction device (6) is connected to the electric pipette gun (1).

3. The improved device for washing and aspirating magnetic beads in a PCR experiment according to claim 1, characterized in that: It also comprises a disposable tube head bin (7) fixedly arranged on the workbench, wherein a plurality of sample holders (5) are mounted on the disposable tube head bin (7), and the sample holders (5) are all arranged in parallel in a horizontal direction.

4. The improved device for washing and aspirating magnetic beads in a PCR experiment according to claim 3, characterized in that: The sample holder (5) may have four holes, eight holes or twelve holes.

5. The improved device for washing and aspirating magnetic beads in PCR experiments according to claim 3, characterized in that: A moving mechanism is arranged in the disposable tube head bin (7) to move the sample holders (5) one by one to a predetermined height, and the predetermined height corresponds to the assembly end of the magnetic frame (8).

6. The improved device for washing and aspirating magnetic beads in PCR experiments according to claim 3, characterized in that: The workbench is provided with a driving mechanism (2) for driving the electric liquid transfer gun (1).

7. The improved device for washing and aspirating magnetic beads in PCR experiments according to claim 1, characterized in that: The suction withdrawal head cover (3) is plugged into a socket at the bottom of the sample holder (5), and the socket is provided with a stopper for limiting the upward movement of the suction withdrawal head cover (3).

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