Sampling needle for in-vitro blastocyst in-vivo examination
By designing a negative pressure suction device with a beveled section and support for the sampling needle for the ex vivo blastocyst biopsy test, the problem of low thermal damage and success rate during the sampling process in the prior art is solved, and rapid and stable biopsy sample acquisition and ex vivo blastocyst integrity protection are achieved.
Patent Information
- Application Number
- CN202421280257.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-05
- Publication Date
- 2025-04-15
- Estimated Expiration
- 2034-06-05
AI Technical Summary
The prior art has problems of thermal damage and low sampling success rate during the sampling process of ex vivo blastocyst biopsy samples. Laser method may lead to thermal damage, while mechanical method sampling needles are prone to shake or slide, resulting in sampling failure.
A sampling needle for ex vivo blastocyst life examination including a sampling needle and a fixing needle is designed. The biopsy sample is obtained quickly and stably and ensures the integrity of the ex vivo blastocyst through a negative pressure suction device and the mechanical shearing method of the first and second oblique surfaces and the support part.
It achieves rapid and stable acquisition of biopsy samples, improves the sampling success rate, avoids thermal damage and the problem of shaking and sliding of the sampling needle, and ensures the integrity of the ex vivo blastocyst.
Smart Images
Figure CN222758228U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of medical instruments, in particular to a sampling needle for in vitro blastocyst living test. Background Art
[0002] In preimplantation genetic testing (PGT), the sampling process of biopsy samples is crucial for the successful transfer of IVF embryos. This technology is mainly used in patients with genetic risks, especially those who face infertility problems due to chromosomal abnormalities, recurrent miscarriages, etc. The sampling of biopsy samples in ex vivo blastocysts of IVF is a technically difficult point, but it is crucial.
[0003] At present, the commonly used biopsy sample sampling methods in clinical practice include laser method and mechanical method. The laser method uses laser to cut the connection between trophoblast cells to obtain cell tissue as a biopsy sample. Usually, 10 to 20 laser shots are required to extract a biopsy sample, and when the cell tissue is tightly connected, the number of laser shots may need to be increased. However, laser shots generate heat, and high-frequency laser shot cutting may cause a certain degree of thermal damage to the in vitro blastocyst, thereby reducing its implantation potential.
[0004] Another commonly used method is the mechanical method, which uses mechanical shear force to cut off the connection between trophoblast cells to obtain cell tissue as a biopsy sample. Compared with the laser method, the mechanical method greatly reduces the use of lasers and effectively protects cells from thermal damage caused by lasers. However, when using the mechanical method for cutting and sampling, the sampling needle is prone to shaking or slipping in the cutting direction, resulting in the inability to complete the mechanical cutting, and may even drag the in vitro blastocyst out of the zona pellucida, causing physical damage to the tissue structure. At the same time, traditional mechanical sampling is mostly performed in a single way. Due to the different strengths of the in vitro blastocysts, it is impossible to flexibly sample different in vitro blastocysts, and the sampling success rate is low. Utility Model Content
[0005] The utility model provides a sampling needle for in vitro blastocyst live inspection, which can quickly and stably obtain a biopsy sample through a mechanical shearing method, ensure the integrity of the in vitro blastocyst after sampling, and improve the sampling success rate.
[0006] In order to achieve the above purpose, the utility model adopts the following technical solutions:
[0007] A sampling needle for in vitro blastocyst live inspection is used for sampling in vitro blastocyst, comprising a sampling needle, a fixing needle and a negative pressure aspirator.
[0008] The sampling needle and the fixing needle are both hollow needle tubes, one end of the sampling needle is provided with a first beveled surface, one end of the fixing needle is provided with a second beveled surface matching the first beveled surface, a supporting portion is provided on the lower side of one end of the fixing needle axially protruding, and the other end of the sampling needle and the other end of the fixing needle are respectively connected to the negative pressure aspirator.
[0009] Optionally, a sampling needle limiting groove is provided on the supporting portion, and the sampling needle limiting groove is arranged along the axial direction of the fixed needle.
[0010] Optionally, the bottom of the sampling needle limiting groove is in an arc shape that matches the outer contour of the sampling needle.
[0011] Optionally, an upper surface of the supporting portion close to an opening at one end of the sampling needle is an arc-shaped surface.
[0012] Optionally, the vacuum inner diameter of the fixing needle is matched with the outer diameter of the sampling needle.
[0013] Optionally, it further includes a movable arm, in which the negative pressure aspirator is arranged, and the other end of the sampling needle is connected to the movable arm, and the movable arm is configured to drive the sampling needle to move up and down and horizontally.
[0014] Optionally, it further includes a fixed arm, in which the negative pressure aspirator is arranged, the other end of the fixed needle is connected to the fixed arm, and the fixed arm and the movable arm are arranged at intervals.
[0015] Optionally, the sampling needle is a 304 stainless steel structural part.
[0016] Compared with the related art, the utility model has the following beneficial effects at least including:
[0017] The sampling needle for in vitro blastocyst biopsy provided by the embodiment of the utility model cooperates with the sampling needle and the fixed needle, each of which is connected to a negative pressure aspirator. First, the in vitro blastocyst is adsorbed by the fixed needle, and the sampling needle is moved to release the in vitro blastocyst to perform suction sampling of the trophoblast cells and overall adsorption of the in vitro blastocyst. After canceling the negative pressure adsorption of the fixed needle, the sampling needle is operated to adsorb the in vitro blastocyst to adjust the relative position with the fixed needle, and the trophoblast cells are cut and separated from the in vitro blastocyst by the abutment sliding of the first bevel and the second bevel from top to bottom. The shear force generated. In the cutting process, the supporting part located on the lower side of the second bevel can limit and support the sampling needle after cutting in place to avoid shaking and sliding. The biopsy sample can be obtained quickly and stably by mechanical shearing, ensuring the integrity of the in vitro blastocyst after sampling, and improving the success rate of sampling. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1A schematic diagram of the structure of the sampling needle for in vitro blastocyst living test provided by the utility model adsorbing the in vitro blastocyst through the fixing needle;
[0019] Figure 2 A schematic diagram of the structure of the sampling needle for in vitro blastocyst living test provided by the utility model, in which the in vitro blastocyst is adsorbed by the sampling needle;
[0020] Figure 3 It is a partial structural schematic diagram of the sampling needle in the utility model;
[0021] Figure 4 It is a partial structural schematic diagram of the fixing needle in the utility model;
[0022] Figure 5 It is a three-dimensional structural schematic diagram of the fixing needle in the utility model;
[0023] Figure 6 It is a three-dimensional structural schematic diagram of another fixing needle in the utility model;
[0024] Figure 7 It is a schematic diagram of the top view of the structure in which the sampling needle in the utility model is overlapped on the fixed needle supporting part;
[0025] Figure 8 This is a schematic diagram of the structure of the sampling needle and the fixing needle in the early stage of beveling when cutting an in vitro blastocyst with relatively loose cell connections according to an embodiment of the utility model;
[0026] Fig. 9 This is a schematic diagram of the structure of the sampling needle and the fixing needle after the oblique cutting is completed when cutting an in vitro blastocyst with relatively loose cell connections according to an embodiment of the utility model;
[0027] Fig.10 This is a schematic structural diagram of the initial stage of beveling of a sampling needle and a fixing needle when cutting an in vitro blastocyst with relatively tight cell connections according to an embodiment of the utility model;
[0028] Fig.11 It is a schematic diagram of the structure of the sampling needle and the fixing needle during the beveling process when cutting an in vitro blastocyst with tightly connected cells according to an embodiment of the utility model;
[0029] Fig.12 A microscopic photograph of the in vitro blastocyst adsorbed by a fixing needle and sampled by a sampling needle provided by the utility model;
[0030] Fig.13 The utility model provides a microscope photograph of the ex vivo blastocyst being cut obliquely using the sampling needle and the fixing needle.
[0031] Legend:
[0032] 1-sampling needle; 2-fixing needle; 3-ex vivo blastocyst; 4-movable arm; 5-negative pressure aspirator; 6-fixing arm; 11-first oblique section; 21-second oblique section; 22-supporting part; 221-sampling needle limiting groove; 31-trophoblast cell. DETAILED DESCRIPTION
[0033] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0034] Figure 1 A schematic diagram of the structure of the sampling needle for in vitro blastocyst living test provided by the utility model adsorbing the in vitro blastocyst through the fixing needle; Figure 2 A schematic diagram of the structure of the sampling needle for in vitro blastocyst living test provided by the utility model, in which the in vitro blastocyst is adsorbed by the sampling needle; Figure 3 It is a partial structural schematic diagram of the sampling needle in the utility model; Figure 4 It is a partial structural schematic diagram of the fixing needle in the utility model; Figure 5 It is a three-dimensional structural schematic diagram of the fixing needle in the utility model; Figure 6 It is a three-dimensional structural schematic diagram of another fixing needle in the utility model; Figure 7 It is a schematic diagram of the top view of the structure in which the sampling needle in the utility model is overlapped on the fixed needle supporting part; Figure 8 This is a schematic diagram of the structure of the sampling needle and the fixing needle in the early stage of beveling when cutting an in vitro blastocyst with relatively loose cell connections according to an embodiment of the utility model; Fig. 9 This is a schematic diagram of the structure of the sampling needle and the fixing needle after the oblique cutting is completed when cutting the in vitro blastocyst with relatively loose cell connection in the embodiment of the utility model; Fig.10 This is a schematic structural diagram of the initial stage of beveling of a sampling needle and a fixing needle when cutting an in vitro blastocyst with relatively tight cell connections according to an embodiment of the utility model; Fig.11 It is a schematic diagram of the structure of the sampling needle and the fixing needle during the beveling process when cutting an in vitro blastocyst with tightly connected cells according to an embodiment of the utility model; Fig.12 A microscopic photograph of the in vitro blastocyst adsorbed by a fixing needle and sampled by a sampling needle provided by the utility model; Fig.13 This is a photo taken under a microscope when the blastocyst is cut obliquely using a sampling needle and a fixing needle provided by the utility model. Figure 1 to Figure 1 As shown, an embodiment of the utility model provides a sampling needle for in vitro blastocyst living inspection, which is used to sample the in vitro blastocyst 3, including a sampling needle 1, a fixing needle 2 and a negative pressure aspirator 5.
[0035] Among them, the sampling needle 1 and the fixing needle 2 are both hollow needle tubes, one end of the sampling needle 1 is provided with a first beveled surface 11, one end of the fixing needle 2 is provided with a second beveled surface 21 matching the first beveled surface 11, and a supporting portion 22 is provided on the lower side of one end of the fixing needle 2 along the axial protrusion, and the other end of the sampling needle 1 and the other end of the fixing needle 2 are respectively connected to the negative pressure suction device 5.
[0036] The sampling needle for in vitro blastocyst live inspection provided by the embodiment of the utility model is first used to start the negative pressure suction device 5 at the other end of the fixed needle 2 to generate negative pressure inside the fixed needle 2, so that the in vitro blastocyst 3 to be sampled is adsorbed at the opening. At this time, the sampling needle 1 is controlled to move manually by the sampling personnel or by manipulating the sampling instrument, and the open end with the first bevel 11 is brought close to the in vitro blastocyst adsorbed on the fixed needle 2. When the first bevel 11 of the sampling needle 1 contacts the in vitro blastocyst 3, the negative pressure suction device 5 connected to the other end is started to suck the trophoblast cells 31 contained in the in vitro blastocyst 3, that is, the embryonic cell tissue required for sampling in this scheme, into the sampling needle 1. After slowly sucking in 3 to 6 trophoblast cells 31 in the in vitro blastocyst 3, the negative pressure suction device 5 is used to reduce the pressure, and the suction is stopped, but the isolated blastocyst 3 is kept adsorbed as a whole on the first bevel 11 end of the sampling needle 1. The actual working scene is referenced Fig.12 At this time, the negative pressure aspirator 5 at the other end of the fixing needle 2 is closed to release the fixing needle 2 from adsorbing the isolated blastocyst 3, and the sampling needle 1 and the adsorbed isolated blastocyst 3 are moved simultaneously by adjusting the movable arm 4 to implement the next step of mechanical cutting sampling operation.
[0037] When cutting and sampling, the sampling needle 1 is controlled to drive the in vitro blastocyst 3 to move together, so that the sampling needle 1 approaches the fixing needle 2 from top to bottom, and the first beveled surface 11 of the sampling needle 1 is in contact with the second beveled surface 21 of the fixing needle 2, and slides downward along the second beveled surface 21 of the fixing needle 2 until the bottom of the first beveled surface 11 of the sampling needle 1 abuts against the supporting portion 22, and the shear force generated during the mutual abutment and sliding process is used to separate the trophoblast cells 31 sucked into the sampling needle 1 from the external in vitro blastocyst 3, and then the sampling needle 1 is controlled to separate from the fixing needle 2, and the first beveled surface 11 is separated from the second beveled surface 21, and finally the controlled negative pressure aspirator 5 on one side of the sampling needle 1 is closed, so that the trophoblast cells 31 of the in vitro blastocyst 3 cut off in the sampling needle 1 can be squeezed out to complete the cutting sampling. For actual working scenes, please refer to Fig.13 The cut in vitro blastocyst 3 structure will also fall onto the supporting portion 22 after the sampling needle 1 is separated. At this time, the negative pressure aspirator 5 can be started to absorb and recover the sampled in vitro blastocyst 3 through the fixed needle 2 again to ensure its integrity and facilitate subsequent other experimental operations.
[0038] The sampling needle for in vitro blastocyst biopsy provided by the embodiment of the utility model cooperates with the sampling needle 1 and the fixed needle 2, each of which is connected to a negative pressure aspirator 5. First, the fixed needle 2 adsorbs the in vitro blastocyst 3, and the sampling needle 1 and the in vitro blastocyst 3 are moved to remove the suction sampling of the trophoblast cells 31 and the overall adsorption of the in vitro blastocyst 3. After the negative pressure adsorption of the fixed needle 2 is cancelled, the sampling needle 1 is operated to adsorb the in vitro blastocyst 3 to adjust the relative position with the fixed needle 2, and the trophoblast cells 31 are cut and separated from the in vitro blastocyst 3 by the abutment and sliding of the first bevel 11 and the second bevel 21 from top to bottom. The shear force generated. In the cutting process, the supporting part 22 located at the lower side of the second bevel 21 can limit and support the sampling needle 1 after cutting in place, so as to avoid shaking and sliding, and can quickly and stably obtain the biopsy sample by mechanical shearing method, ensure the integrity of the in vitro blastocyst 3 after sampling, and improve the success rate of sampling.
[0039] For example, in the above description, the in vitro blastocyst 3 is cut and sampled only by the mutual abutment and sliding of the first bevel 11 and the second bevel 21, which is generally suitable for in vitro blastocysts 3 with loose cell connections, while for in vitro blastocysts 3 with tighter connections, the adsorbed trophoblast cells 31 may not be completely separated from the external in vitro blastocysts 3 by the above operation alone. In this case, the sampling needle for in vitro blastocyst live inspection provided by the embodiment of the utility model can also achieve stable cutting by performing corresponding fine-tuning of the operation. Fig. 9 After canceling the negative pressure adsorption of the fixing needle 2, operate the sampling needle 1 to adsorb the in vitro blastocyst 3 and adjust the relative position with the fixing needle 2, and slide to the bottom from top to bottom through the abutment of the first bevel 11 and the second bevel 21. After the sampling needle 1 abuts against the supporting part 22, keep the controlled negative pressure aspirator 5 on one side of the sampling needle 1 turned on for adsorption, and at the same time turn on the negative pressure aspirator 5 on the side of the fixing needle 2 to adsorb the in vitro blastocyst 3. At the same time, the sampling needle 1 continues to be pressed down. The sampling needle 1 made of 304 stainless steel has good ductility and mechanical strength. Under the needle-like structure with a relatively thin overall diameter, it is squeezed with the supporting part 22 as a fulcrum to produce a certain bending elastic deformation. At this time, the sampling needle 1 is quickly pulled away from the fixing needle 2 along the axial direction. At the moment when the first beveled surface 11 of the sampling needle 1 leaves the second beveled surface 21 of the fixing needle 2, the sampling needle 1 will be straightened by the elastic restoring force, thereby generating radial shear force, so that the trophoblast cells 31 are separated from the in vitro blastocyst 3.
[0040] Optionally, in the embodiment of the utility model, in conjunction with the installation of the sampling needle 1 and the fixing needle 2, a movable arm 4 and a fixed arm 6 are respectively provided, and the insides of the two provide sealed installation positions for the negative pressure aspirator 5 corresponding to the sampling needle 1 and the fixing needle 2, respectively, and are arranged at intervals on a specially set sampling station to facilitate the sampling personnel to operate. When performing the coordinated cutting operation of the sampling needle 1 and the fixing needle 2, the movable arm 4 is a mechanical arm driven by an external motor structure, and is configured to drive the sampling needle 1 to lift and move horizontally, thereby adjusting the relative position of the first bevel 11 and the second bevel 21 of the fixing needle 2, and realizing the adsorption and cutting of the in vitro blastocyst 3.
[0041] Optionally, a sampling needle limiting groove 221 is provided on the supporting portion 22, and the sampling needle limiting groove 221 is arranged along the axial direction of the fixed needle 2. For example, in the embodiment of the utility model, by providing the sampling needle limiting groove 221 on the supporting portion 22, when the sampling needle 1 cuts the in vitro blastocyst 3 from top to bottom through the first bevel 11 and the second bevel 21, it will be inserted into the sampling needle limiting groove 221 on the supporting portion 22. Figure 7 The sampling needle limiting groove 221 is used to limit the lower outer side surface of the sampling needle 1, further ensuring contact stability, avoiding shaking or sliding, and further improving the stability of the sampling process.
[0042] Optionally, the bottom of the sampling needle limiting groove 221 is in an arc shape that matches the outer contour of the sampling needle 1. For example, in the embodiment of the utility model, by setting the bottom of the sampling needle limiting groove 221 to an arc shape, after the sampling needle 1 is inserted, it can be more closely fitted with the outer side surface of the pre-sampling needle, further improving the contact stability, thereby improving the stability of the sampling process.
[0043] Optionally, the upper surface of the supporting portion 22 near the opening at one end of the sampling needle 1 is an arc-shaped surface. Exemplarily, in the embodiment of the utility model, by setting the upper surface of the supporting portion 22 near the opening at one end of the sampling needle 1 to be an arc-shaped surface, in addition to the sampling needle limiting groove 221 for correspondingly supporting the sampling needle 1, when the sampling needle 1 contacts other positions of the supporting portion 22, or when the in vitro blastocyst 3 contacts the upper surface of the supporting portion 22 after sampling is completed, it can be smoother, avoiding damage by bumps, or causing the in vitro blastocyst 3 to rupture, further improving the practicality of the sampling needle for in vitro blastocyst live inspection.
[0044] Optionally, the vacuum inner diameter of the fixing needle 2 is adapted to the outer diameter of the sampling needle 1. For example, in the embodiment of the utility model, when the cells of the in vitro blastocyst 3 are tightly connected, the first bevel 11 and the second bevel 21 are abutted against each other and slided, and the elastic operation and cutting of the sampling needle 1 still cannot completely separate the adsorbed trophoblast cells 31 from the external in vitro blastocyst 3. Fig.11By matching the vacuum inner diameter of the fixing needle 2 with the outer diameter of the sampling needle 1, after the trophoblast cells 31 are adsorbed inside by the sampling needle 1, the sampling needle 1 is controlled along the radial direction of the fixing needle 2 to completely penetrate one end of the sampling needle 1 into the negative pressure opening of the fixing needle 2, and the shear force generated by the contact between the outer wall of the sampling needle 1 and the inner wall of the opening of the fixing needle 2 is used to completely physically separate the trophoblast cells 31 from the external ex vivo blastocyst 3. In this way, the ex vivo blastocyst 3 with different strengths can be sampled flexibly, the overall sampling success rate is improved, and the practicality of the sampling needle for ex vivo blastocyst live inspection is further increased.
[0045] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.
Claims
1. A sampling needle for in vitro blastocyst biopsy, used for sampling in vitro blastocyst (3), characterized in that: It comprises a sampling needle (1), a fixing needle (2) and a negative pressure aspirator (5). The sampling needle (1) and the fixing needle (2) are both hollow needle tubes. One end of the sampling needle (1) is provided with a first beveled surface (11), and one end of the fixing needle (2) is provided with a second beveled surface (21) matching the first beveled surface (11). A supporting portion (22) is provided on the lower side of one end of the fixing needle (2) protruding along the axial direction. The other end of the sampling needle (1) and the other end of the fixing needle (2) are respectively connected to the negative pressure aspirator (5).
2. The sampling needle for in vitro blastocyst biopsy according to claim 1, characterized in that: The supporting portion (22) is provided with a sampling needle limiting groove (221), and the sampling needle limiting groove (221) is arranged along the axial direction of the fixed needle (2).
3. The sampling needle for in vitro blastocyst biopsy according to claim 2, characterized in that: The bottom of the sampling needle limiting groove (221) is in an arc shape that matches the outer contour of the fixing needle (2).
4. The sampling needle for in vitro blastocyst biopsy according to claim 3, characterized in that: The upper surface of the supporting portion (22) close to the opening at one end of the sampling needle (1) is an arc-shaped surface.
5. The sampling needle for in vitro blastocyst biopsy according to any one of claims 1 to 4, characterized in that: The vacuum inner diameter of the fixing needle (2) is matched with the outer diameter of the sampling needle (1).
6. The sampling needle for in vitro blastocyst biopsy according to any one of claims 1 to 4, characterized in that: It also comprises a movable arm (4), in which the negative pressure aspirator (5) is arranged, and the other end of the sampling needle (1) is connected to the movable arm (4), and the movable arm (4) is configured to drive the sampling needle (1) to move up and down and horizontally.
7. The sampling needle for in vitro blastocyst biopsy according to claim 6, characterized in that: It also comprises a fixed arm (6), in which the negative pressure suction device (5) is arranged, and the other end of the fixed needle (2) is connected to the fixed arm (6), and the fixed arm (6) and the movable arm (4) are arranged at intervals.
8. The sampling needle for in vitro blastocyst biopsy according to any one of claims 1 to 4, characterized in that: The sampling needle (1) is a 304 stainless steel structural part.