Microfluidic immunomagnetic bead analysis system

Abstract

The utility model relates to the technical field of micro-fluidic instant detection. The microfluidic immunomagnetic bead analysis system comprises an instrument body, the instrument body comprises a shell, the shell is internally provided with: a microfluidic chip module comprising a substrate and a reaction chamber arranged on the substrate; the reaction device is characterized by further comprising a vibration module independently acting on the reaction bin; the magnetic attraction module comprises an electromagnet acting on the bottom of the reaction bin and an electromagnet support connecting the electromagnet and the shell, the electromagnet support at least partially surrounds the lower end of the electromagnet, and heat dissipation holes for discharging heat generated by the electromagnet are formed in the electromagnet support. The microfluidic immunomagnetic bead analysis system has the advantages of good heat dissipation and high detection precision.

Description

Technical Field

[0001] The utility model relates to the technical field of microfluidic instant detection, in particular to a microfluidic immunomagnetic bead analysis system. Background Art

[0002] With the development of science and technology, the demand for home health testing has increased, and the demand for home testing has expanded, making POCT testing one of the main development directions in the field of testing in recent years. POCT refers to on-site sampling, on-site detection of results, and the use of portable and compact analytical instruments and supporting reagents to quickly obtain the target test object. As a result, microfluidic detection methods have gradually developed. Microfluidics refers to the technology of integrating a series of biochemical experimental processes into a chip. It has the advantages of small size and small injection volume, but only one microfluidic chip cannot achieve both immune response and detection at the same time. Large instruments are still needed to detect the products of immune response.

[0003] In response to this problem, the Chinese utility model patent "A modular bioanalysis system based on droplet array chip and its application" with patent number CN201710978928.0 discloses a system that integrates the immune reaction and detection process into one system. The system includes an optical detection module and a reaction control module. The position of the optical detection module corresponds to the microfluidic chip detection pool and is used to transmit and receive light signals. The reaction control module applies an external field to regulate the biochemical reaction in the reaction pool, including a magnetic field control module, a chip motion control module and a temperature control module. The magnetic field control module controls the adsorption and release of immune magnetic beads in the reaction pool. The chip control module promotes the reaction in the reaction pool by driving the chip to reciprocate in a small amplitude and high frequency. The temperature control module controls the temperature of the microfluidic chip reaction pool and each channel. The system can realize full automation from sample loading to the end of detection.

[0004] Although the system in the above patent has realized the integration of immune detection function, its temperature control module can only be used to control the temperature of the microfluidic chip. After a long period of operation of the system, the high temperature environment caused by the heating of the circuit board or the components of each module will affect the service life of the adjacent modules; the integration of the magnetic field control module leads to its small size and small heat dissipation area, making it difficult for the Joule heat generated by the current to be transmitted to the outside world, resulting in frequent overheating and affecting the reaction rate in the microfluidic chip; in addition, the oscillation and mixing process of the reactants in the reaction pool in the above patent is achieved by driving the entire chip, which has an impact on some immune reactions that require the reaction pool and the detection pool to be separated during detection, such as enzyme-linked immunosorbent assay. During the oscillation process, the intermediate products that are not fully reacted in the reaction pool will flow into the detection pool along the channel, thereby affecting the detection results. Utility Model Content

[0005] The technical problem to be solved by the utility model is to provide a microfluidic immunomagnetic bead analysis system which can not only dissipate heat for a magnetic field control module but also vibrate a reaction chamber specifically in response to the above-mentioned existing technical status.

[0006] The technical solution adopted by the utility model to solve the above technical problems is: the microfluidic immunomagnetic bead analysis system includes an instrument body, the instrument body includes a shell, and the shell is provided with:

[0007] The microfluidic chip module comprises a substrate and a reaction chamber arranged on the substrate, wherein an inlet for air or liquid is provided at the upstream of the reaction chamber, and a detection chamber is connected to the downstream of the reaction chamber, and an outlet for air and liquid is provided at the downstream of the detection chamber;

[0008] It is characterized by also including:

[0009] A vibration module, acting independently on the reaction chamber, for accelerating the incubation reaction in the reaction chamber;

[0010] The magnetic attraction module includes an electromagnet acting on the bottom of the reaction chamber and an electromagnet bracket connecting the electromagnet and the shell. The electromagnet bracket at least partially surrounds the lower end of the electromagnet and is provided with a heat dissipation hole for dissipating the heat generated by the power supply magnet.

[0011] In order for the vibration module to stably act on the reaction chamber, from the perspective of simple structure, preferably, the vibration module includes:

[0012] A vibration module bracket connected to the housing;

[0013] The vibration element is hingedly connected to the vibration module bracket, and includes a first end hingedly connected to the vibration module bracket and a second end for vibrating the reaction chamber; correspondingly,

[0014] The housing includes a bottom wall for connecting the vibration module bracket and a side wall surrounding the periphery of the bottom wall, and the two together form a cavity that is fluidically connected to the heat dissipation hole. The arrangement of the vibration module bracket stabilizes the connection between the vibration module and the housing, and the hinged connection between the vibration element and the vibration module bracket enables the user to manually adjust the vibration element to be close to the reaction chamber, so that the range of movement of the vibration element becomes larger.

[0015] In order to fully dissipate the heat of the electromagnet in the cavity by convection, preferably, a support seat for supporting the microfluidic chip module is provided in the cavity of the shell, and the support seat is connected to the bottom wall; a mounting seat for mounting the magnetic module is provided in the support seat, the vibration module bracket is provided on one of the side walls of the support seat, and a connecting portion is left on the support seat for the mounting seat to communicate with the cavity fluid. The heat of the electromagnet is exchanged with the cavity in the form of heat convection through the heat dissipation holes via the connecting portion, which increases the heat dissipation efficiency of the system and reduces the risk of excessive temperature of the electromagnet.

[0016] In order to realize that the vibration element acts independently on the reaction chamber, preferably, the vibration module bracket and the vibration element are rotatably connected through a bracket arm, and correspondingly, the bracket arm is provided with a first rotating part for connecting the first end of the reaction chamber and a second rotating part connected to the vibration module bracket. The setting of the bracket arm further increases the movement range of the vibration element above the microfluidic chip module, thereby controlling the alignment of the vibration element and the reaction chamber.

[0017] Furthermore, a detection module corresponding to the detection chamber is also arranged on the support seat, and the detection module is connected to the support seat via a connecting bracket. A sensor for detecting immune response signals in the detection chamber is arranged in the detection module.

[0018] In order to integrate the detection module in the system and prevent interference from external light sources, preferably, the detection module includes:

[0019] An emission platform is arranged between the detection chamber and the mounting seat and covers the detection chamber. An emission sensor opposite to the detection chamber is arranged on the emission platform. The emission sensor serves as a light source and can adjust the wavelength of the light source.

[0020] The receiving platform is arranged on the detection chamber and covers the detection chamber. The receiving platform is provided with a receiving sensor cooperating with the transmitting sensor. The receiving sensor is used to receive the light signal emitted by the transmitting sensor and passing through the detection chamber. The sensor includes the transmitting sensor and the receiving sensor. The transmitting platform and the receiving platform cover the detection chamber to ensure that the external light source will not affect the detection result when the detection module is working. The transmitting platform is arranged between the detection chamber and the mounting seat to make full use of the space of the mounting seat and reduce the volume of the system.

[0021] Compared with the prior art, the advantages of the utility model are: the microfluidic immunomagnetic bead analysis system uses targeted vibration of the reaction chamber instead of the vibration of the entire microfluidic chip in the prior art, thereby avoiding the situation where the immunomagnetic beads flow out of the reaction chamber without reacting with the substance to be tested, resulting in a false negative; the heat dissipation holes on the electromagnet bracket increase the heat exchange area between the electromagnet and the outside world, reduce the working temperature of the electromagnet, ensure the good operation of the microfluidic biological analysis system and the effectiveness of the detection results, and have the advantages of good heat dissipation and high detection accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the structure of an embodiment of the utility model;

[0023] Figure 2 It is a schematic diagram of the internal structure of an embodiment of the utility model;

[0024] Figure 3 It is a structural explosion diagram of an embodiment of the utility model;

[0025] Figure 4 It is a structural exploded diagram of the microfluidic chip module of an embodiment of the utility model;

[0026] Figure 5 A schematic diagram of the structure of a substrate in an embodiment of the utility model;

[0027] Figure 6 This is a schematic diagram of the structure of the support base and the magnetic attraction module of an embodiment of the utility model;

[0028] Figure 7 This is a schematic diagram of the structure of a vibration module according to an embodiment of the utility model;

[0029] Figure 8 A longitudinal sectional view of an embodiment of the utility model;

[0030] Fig. 9 for Figure 8 A partial enlarged view of the middle A;

[0031] Fig.10 A longitudinal cross-sectional view from another angle of an embodiment of the utility model;

[0032] Fig.11 for Fig.10 A partial enlarged view of point B in the middle. DETAILED DESCRIPTION

[0033] The present invention will be described in further detail below in conjunction with the accompanying drawings.

[0034] like Figures 1 to 11The figure shows the best embodiment of the utility model. The microfluidic immunomagnetic bead analysis system of this embodiment is mainly used to detect the concentration of biological samples. When the system is used for field sampling, the microfluidic immunomagnetic bead analysis system must provide users with a smaller volume and sensitive and rapid detection to facilitate outdoor carrying and rapid detection results after sampling. However, the smaller volume will lead to uneven heat convection inside the system, resulting in excessive local temperature and affecting the normal operation of the system. Therefore, it will be beneficial to provide a microfluidic immunomagnetic bead analysis system with heat dissipation function while ensuring small volume and fast and accurate detection. The following will expand the structural description of the microfluidic immunomagnetic bead analysis system:

[0035] See also Figures 1 to 5 The present microfluidic immunomagnetic bead analysis system includes an instrument body, which includes a shell 1, in which a microfluidic chip module 2 is arranged, and the microfluidic chip module 2 includes a substrate 21 and a reaction chamber 22 arranged on the substrate 21, and an inlet 23 for air or liquid is provided upstream of the reaction chamber 22, and a detection chamber 24 is connected downstream of the reaction chamber 22, and an outlet 25 for air and liquid is provided downstream of the detection chamber 24; the instrument body also includes a vibration module 3 and a magnetic attraction module 4, wherein the vibration module 3 acts independently on the reaction chamber 22 to accelerate the incubation reaction in the reaction chamber 22; the magnetic attraction module 4 includes an electromagnet 41 acting on the bottom of the reaction chamber 22 and an electromagnet bracket 42 connecting the electromagnet 41 and the shell 1, and the electromagnet bracket 42 at least partially surrounds the lower end of the electromagnet 41, and is provided with a heat dissipation hole 421 for discharging the heat generated by the power supply magnet 41. The working state of the vibration element 32 can be controlled manually.

[0036] See also Figure 6 In the housing 1 of this embodiment, a support seat 6 for supporting the microfluidic chip module 2 is provided in the housing 13. The support seat 6 is connected to the bottom wall 11. A mounting seat 61 for mounting the magnetic module 4 is provided in the support seat 6. The vibration module bracket 31 is provided on one of the side walls of the support seat 6. A connecting portion 62 for fluid communication between the mounting seat 61 and the housing 13 is provided on the support seat 6. The heat of the electromagnet 41 is exchanged with the housing 13 in the form of heat convection through the heat dissipation holes 421 via the connecting portion 62, thereby increasing the heat dissipation efficiency of the system, allowing the heat of the electromagnet 41 to be fully dissipated by convection in the housing 13, and reducing the risk of the electromagnet 41 being overheated.

[0037] See also Figure 7In this embodiment, the vibration module bracket 31 and the vibration element 32 are rotatably connected through the bracket arm 33, and the bracket arm 33 is provided with a first rotating portion 331 for connecting the first end of the reaction chamber 22 and a second rotating portion 332 connected to the vibration module bracket 31. The arrangement of the bracket arm 33 further increases the range of motion of the vibration element 32 above the microfluidic chip module 2, thereby controlling the vibration element 32 to align with the reaction chamber 22, so as to achieve the vibration element 32 acting on the reaction chamber 22 independently.

[0038] See also Figures 8 to 11 A detection module 5 corresponding to the detection chamber 24 is also arranged on the support seat 6 . The detection module 5 is connected to the support seat 6 via a connecting bracket. A sensor for detecting immune response signals in the detection chamber 24 is arranged in the detection module 5 .

[0039] The detection module 5 of this embodiment includes a transmitting platform 51 and a receiving platform 52, wherein the transmitting platform 51 is arranged between the detection chamber 24 and the mounting seat 61 and covers the detection chamber 24, and an transmitting sensor 511 opposite to the detection chamber 24 is arranged on the transmitting platform 51, and the transmitting sensor 511 serves as a light source and can adjust the wavelength of the light source; the receiving platform 52 is arranged on the detection chamber 24 and covers the detection chamber 24, and a receiving sensor 521 cooperating with the transmitting sensor 511 is arranged on the receiving platform 52, and the receiving sensor 521 is used to receive the light signal emitted by the transmitting sensor 511 and passing through the detection chamber 24, and the sensor includes the transmitting sensor 511 and the receiving sensor 521. The transmitting platform 51 and the receiving platform 52 cover the detection chamber 24 to ensure that the external light source will not affect the detection result when the detection module 5 is working, and the transmitting platform 51 is arranged between the detection chamber 24 and the mounting seat 61 to make full use of the space of the mounting seat 61 and reduce the volume of the system.

[0040] The working process of the microfluidic immunomagnetic bead analysis system of this embodiment is as follows: open the shell 1 and adjust the head of the vibration element 32 to face the reaction chamber 22 through the vibration module bracket 31, close the shell 1, add magnetic beads and primary antibody solution into the reaction chamber 22 from the inlet 23, turn on the vibration module 3 to vibrate and incubate to accelerate the coupling reaction, and after the magnetic beads and the primary antibody are coupled, close the vibration element 32 and turn on the magnetic suction module 4, the electromagnet 41 generates a magnetic field to magnetically separate the magnetic beads and the primary antibody coupling from the solution to the bottom of the reaction chamber 22; let air in from the inlet 23 to empty the waste liquid in the reaction chamber 22, add the enzyme-labeled secondary antibody solution into the reaction chamber 22, and close the shell 1. Close the magnetic suction module 4 and then open the vibration element 3 to vibrate and incubate to accelerate the coupling reaction. After the coupling of the magnetic bead primary antibody coupling product and the enzyme-labeled secondary antibody is completed, close the vibration module 3 and open the magnetic suction module 4, and magnetically absorb the magnetic bead primary antibody-enzyme-labeled secondary antibody coupling product to the bottom of the reaction chamber, and let air in from the inlet 23 to drain the waste liquid in the reaction chamber 22. Add the color developing solution and the stop solution into the reaction chamber 22 in turn and close the magnetic suction module 4. After the termination reaction is completed, the liquid to be detected is obtained, and air is introduced from the inlet 23 to push the liquid to be detected in the reaction chamber 22 to the detection chamber 24. Open the detection module 5 to detect the absorbance of the liquid to be detected and record the data.

Claims

1. A microfluidic immunomagnetic bead analysis system, comprising an instrument body, wherein the instrument body comprises a housing (1), wherein the housing (1) is provided with: A microfluidic chip module (2) comprises a substrate (21) and a reaction chamber (22) arranged on the substrate (21), wherein an inlet (23) for air or liquid is provided upstream of the reaction chamber (22), and a detection chamber (24) is connected downstream of the reaction chamber (22), and an outlet (25) for air or liquid is provided downstream of the detection chamber (24); Features: Also included are: A vibration module (3), acting independently on the reaction chamber (22), for accelerating the incubation reaction in the reaction chamber (22); The magnetic attraction module (4) comprises an electromagnet (41) acting on the bottom of the reaction chamber (22) and an electromagnet bracket (42) connecting the electromagnet (41) and the housing (1); the electromagnet bracket (42) at least partially surrounds the lower end of the electromagnet (41) and is provided with a heat dissipation hole (421) for discharging heat generated by the power supply magnet (41).

2. The microfluidic immunomagnetic bead analysis system according to claim 1, characterized in that: The vibration module (3) comprises: A vibration module bracket (31) connected to the housing (1); The vibration element (32) is hingedly connected to the vibration module bracket (31), and comprises a first end hingedly connected to the vibration module bracket (31) and a second end for vibrating the reaction chamber (22); correspondingly, The housing (1) comprises a bottom wall (11) for connecting the vibration module bracket (31), and a side wall (12) surrounding the periphery of the bottom wall (11), and the two together form a cavity (13) that is fluidically connected to the heat dissipation hole (421).

3. The microfluidic immunomagnetic bead analysis system according to claim 2, characterized in that: A support seat (6) for supporting the microfluidic chip module (2) is arranged in the cavity (13) of the shell (1), and the support seat (6) is connected to the bottom wall (11); a mounting seat (61) for mounting the magnetic suction module (4) is arranged in the support seat (6), the vibration module bracket (31) is arranged on one of the side walls of the support seat (6), and a connecting portion (62) is reserved on the support seat (6) for fluid communication between the mounting seat (61) and the cavity (13).

4. The microfluidic immunomagnetic bead analysis system according to claim 2, characterized in that: The vibration module bracket (31) and the vibration element (32) are rotatably connected via a bracket arm (33); correspondingly, the bracket arm (33) is provided with a first rotating portion (331) to which the first end of the vibration element (32) is connected, and a second rotating portion (332) connected to the vibration module bracket (31).

5. The microfluidic immunomagnetic bead analysis system according to claim 3, characterized in that: The support seat (6) is also provided with a detection module (5) corresponding to the detection chamber (24); the detection module (5) is connected to the support seat (6) via a connecting bracket; and a sensor for detecting immune response signals in the detection chamber (24) is provided in the detection module (5).

6. The microfluidic immunomagnetic bead analysis system according to claim 5, characterized in that: The detection module (5) comprises: An emission platform (51) is arranged between the detection chamber (24) and the mounting seat (61) and covers the detection chamber (24); an emission sensor (511) is arranged on the emission platform (51) and is opposite to the detection chamber (24); the emission sensor (511) serves as a light source and can adjust the wavelength of the light source; A receiving platform (52) is arranged on the detection chamber (24) and covers the detection chamber (24). A receiving sensor (521) cooperating with the transmitting sensor (511) is arranged on the receiving platform (52). The receiving sensor (521) is used to receive the optical signal emitted by the transmitting sensor (511) and passing through the detection chamber (24). The sensor includes the transmitting sensor (511) and the receiving sensor (521).

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