Cell reaction liquid sampling equipment

Abstract

The utility model discloses cell reaction liquid sampling equipment which comprises a box body, a plurality of dropping pipettes, a plurality of peristaltic pumps and a supporting seat used for supporting a pore plate culture dish, an X-axis guide rail is arranged at the bottom of the box body, a sliding block is arranged on the X-axis guide rail in a sliding mode, the sliding block is provided with a Y-axis guide rail, and the supporting seat is connected along the Y-axis guide rail in a sliding mode. The dropping pipettes are located right above the supporting seat, one end of each peristaltic pump is connected with the corresponding dropping pipette through a first connecting pipe, and the other end of each peristaltic pump is connected with the bioreactor through a second connecting pipe. The liquid taking device has the advantages that liquid taking is convenient, efficiency is high, the liquid taking amount can be controlled, and reaction liquid cannot be wasted. And the reaction liquid is not polluted.

Description

Technical Field

[0001] The utility model relates to the technical field of automation equipment, in particular to a cell reaction liquid sampling device. Background Art

[0002] In order to detect the environment of cell reaction, the cell reaction liquid needs to be tested. First, the cell reaction liquid needs to be taken out and then sent to the detection equipment for detection. The existing method is to manually take out the cell reaction liquid and then send it to the detection equipment for detection. This method of taking liquid is troublesome, inefficient, and the amount of liquid taken is not easy to control. It may also contaminate the reaction liquid. Utility Model Content

[0003] The technical problem to be solved by the utility model is to provide a cell reaction liquid sampling device in view of the deficiencies of the prior art.

[0004] In order to solve the above technical problems, the utility model adopts the following technical solutions.

[0005] A cell reaction liquid sampling device comprises a box body, a plurality of dropper tubes, a plurality of peristaltic pumps and a support base for supporting a well plate culture dish, wherein an X-axis guide rail is arranged at the bottom of the box body, a slider is slidably arranged on the X-axis guide rail, the slider is provided with a Y-axis guide rail, the support base is slidably connected along the Y-axis guide rail, the dropper tube is located directly above the support base, one end of each peristaltic pump is connected to a corresponding dropper tube through a first connecting tube, and the other end is connected to a bioreactor through a second connecting tube.

[0006] A preferred solution is that a waste liquid collection tank is provided on one side of the support base.

[0007] A preferred solution is that the support seat is provided with a fixing groove, the fixing groove is used to fix the well plate culture dish, and there is a gap between the fixing groove and the waste liquid collection groove.

[0008] A preferred solution is that the cell reaction liquid sampling device also includes a three-way valve and a clamp valve, the first port of the three-way valve is connected to the peristaltic pump, the second port of the three-way valve is connected to the second connecting pipe, and the third port of the three-way valve is connected to the clamp valve.

[0009] A preferred solution is that the cell reaction liquid sampling device further comprises a second liquid sensor and a first liquid sensor, wherein the second liquid sensor is arranged between the three-way valve and the peristaltic pump, and the first liquid sensor is arranged between the peristaltic pump and the dropper.

[0010] A preferred solution is that the pinch valve is connected to a compressed air machine.

[0011] A cell reaction liquid sampling device provided by the embodiment of the utility model has at least the following beneficial effects: each peristaltic pump corresponds to a dropper, the support seat can move along the X-axis and Y-axis directions in the box, the orifice plate culture dish is provided with a plurality of transverse and longitudinal culture holes, the support seat can drive the orifice plate culture dish to move along the X-axis and Y-axis directions, when the peristaltic pump is working, the peristaltic pump draws the reaction liquid from the external container, the reaction liquid flows to the dropper along the peristaltic pump, and the dropper infuses the reaction liquid into the culture hole corresponding to the orifice plate culture dish. When one of the culture holes is filled with the reaction liquid, the orifice plate culture dish moves along the X-axis and Y-axis directions with the support seat, and the dropper infuses the reaction liquid into another culture hole, and the reaction liquid can be infused into the culture hole of the entire orifice plate culture dish. It is convenient to take the liquid, the efficiency is high, the amount of liquid taken can be controlled, and the reaction liquid will not be wasted. The reaction liquid will not be contaminated.

[0012] The above description is only an overview of the technical solution of the utility model. In order to more clearly understand the technical means of the utility model, it can be implemented in accordance with the contents of the specification. In order to make the above and other purposes, features and advantages of the utility model more obvious and easy to understand, the following preferred embodiments are specifically cited and described in detail with the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a three-dimensional diagram of the utility model;

[0014] Figure 2 It is a three-dimensional structure inside the utility model. Figure 1 ;

[0015] Figure 3 It is a three-dimensional structure inside the utility model. Figure 2 ;

[0016] Figure 4 It is a three-dimensional structure inside the utility model. Figure 3 ;

[0017] Figure 5 It is a pipeline diagram of the utility model. DETAILED DESCRIPTION

[0018] In order to explain the idea and purpose of the present application, the present application will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0019] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by technicians in the technical field of this application; the terms used in the specification of the application herein are only for the purpose of describing specific embodiments and are not intended to limit this application; the terms "including" and "having" and any variations thereof in the specification and claims of this application and the above-mentioned drawings are intended to cover non-exclusive inclusions. The terms "first", "second", "left", "right", etc. in the specification and claims of this application or the above-mentioned drawings are used to distinguish different objects, not to describe a specific order.

[0020] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0021] like Figures 1 to 5 As shown, a cell reaction liquid sampling device includes a box body 10, a plurality of dropper tubes 20, a plurality of peristaltic pumps 30 and a support base 40 for supporting a well plate culture dish 50, the box body 10 is provided with an X-axis guide rail 11 at the bottom, a slider 12 is slidably provided on the X-axis guide rail 11, the slider 12 is provided with a Y-axis guide rail 13, the support base 40 is slidably connected along the Y-axis guide rail 13, the dropper tube 20 is located directly above the support base 40, one end of each peristaltic pump 30 is connected to a corresponding dropper tube 20 through a first connecting tube 31, and the other end is connected to a bioreactor 33 through a second connecting tube 32.

[0022] like Figures 1 to 5 As shown, the cell reaction liquid sampling device also includes a first motor 14 and a second motor 15 . The first motor 14 drives the slider 12 to slide along the X-axis guide rail 11 , and the second motor 15 drives the support seat 40 to slide along the Y-axis guide rail 13 .

[0023] like Figures 1 to 5As shown, the bioreactor 33 is filled with biological reaction liquid, each peristaltic pump 30 corresponds to a dropper 20, the support seat 40 can move along the X-axis and Y-axis directions in the box 10, the orifice plate culture dish 50 is provided with a plurality of transverse and longitudinal culture holes, and the support seat 40 can drive the orifice plate culture dish 50 to move along the X-axis and Y-axis directions. When the peristaltic pump 30 is working, the peristaltic pump 30 draws the biological reaction liquid from the bioreactor 33, and the biological reaction liquid flows along the peristaltic pump 30 to the dropper 20, and the dropper 20 infuses the biological reaction liquid into the culture hole corresponding to the orifice plate culture dish 50. When one of the culture holes is filled with the biological reaction liquid, the orifice plate culture dish 50 moves along the X-axis and Y-axis directions with the support seat 40, and the dropper 20 infuses the biological reaction liquid into another culture hole, and the biological reaction liquid can be infused into the culture holes of the entire orifice plate culture dish 50.

[0024] like Figures 1 to 5 As shown, a waste liquid collection tank 41 is provided on one side of the support base 40. When the peristaltic pump 30 is working, the peristaltic pump 30 first extracts part of the biological reaction liquid, and the part of the biological reaction liquid is first collected in the waste liquid collection tank 41. Afterwards, the biological reaction liquid is infused into the culture wells corresponding to the well plate culture dish 50 to remove part of the sample that may be contaminated at the beginning.

[0025] like Figures 1 to 5 As shown, the support base 40 is provided with a fixing groove 42, and the fixing groove 42 is used to fix the well plate culture dish 50, and there is a gap between the fixing groove 42 and the waste liquid collection groove 41. The well plate culture dish 50 can be placed in the fixing groove 42, which is convenient for removing the well plate culture dish 50, and the liquid in the waste liquid collection groove 41 will not interfere with the well plate culture dish 50.

[0026] like Figures 1 to 5 As shown, the cell reaction liquid sampling device also includes a three-way valve 70 and a clamp valve 60, wherein the first port of the three-way valve 70 is connected to the peristaltic pump 30, the second port of the three-way valve 70 is connected to the second connecting tube 32, and the third port of the three-way valve 70 is connected to the clamp valve 60.

[0027] like Figures 1 to 5 As shown, the cell reaction liquid sampling device further includes a second liquid sensor 82 and a first liquid sensor 81 . The second liquid sensor 82 is arranged between the three-way valve 70 and the peristaltic pump 30 , and the first liquid sensor 81 is arranged between the peristaltic pump 30 and the drip tube.

[0028] like Figures 1 to 5 As shown, the pinch valve 60 is connected to a compressed air machine 61. When the pinch valve 60 is opened, the compressed air machine 61 blows the liquid in the second connecting pipe 32 back to the bioreactor 33 to avoid waste and loss of the reaction liquid.

[0029] like Figures 1 to 5As shown, when sampling begins, the pinch valve 60 is closed, the peristaltic pump 30 starts to rotate, and the liquid in the bioreactor 33 is pumped up.

[0030] like Figures 1 to 5 As shown, when the liquid flows through the second liquid sensor 82, the timing starts. After a sufficient amount of liquid is taken, the pinch valve 60 is opened, and the gas flows through the pinch valve 60 to press the liquid in the second connecting tube 32 back into the reactor.

[0031] like Figures 1 to 5 As shown, the liquid in the first port of the three-way valve 70 connected to the peristaltic pump 30 continues to move along the first connecting tube 31, and the timing starts after flowing through the first liquid sensor 81. After ensuring that all the liquid in the first connecting tube 31 is taken to the orifice plate culture dish 50, the clamping valve 60 is closed and the peristaltic pump 30 stops rotating.

[0032] like Figures 1 to 5 As shown, the temperature in the box 10 is maintained at about 4 degrees, which can keep the sample cold and improve the automation and manual intervention time;

[0033] like Figures 1 to 5 As shown, the first connecting tube 31 and the second connecting tube 32 are clamped on the outer wall of the box body 10, and the first connecting tube 31 and the second connecting tube 32 are detachable, which is convenient for midway disassembly for sterilization and disinfection.

[0034] like Figures 1 to 5 As shown, by adding a waste liquid collection tank 41, the sample capacity that may be initially contaminated can be flexibly designed to be removed to ensure sampling accuracy.

[0035] The above is a specific implementation of the present utility model. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present utility model. These improvements and modifications are also considered to be within the protection scope of the present utility model.

Claims

1. A cell reaction liquid sampling device, characterized in that: It includes a box body, a plurality of dripping tubes, a plurality of peristaltic pumps and a support base for supporting a well plate culture dish. The bottom of the box body is provided with an X-axis guide rail, a slider is slidably provided on the X-axis guide rail, the slider is provided with a Y-axis guide rail, the support base is slidably connected along the Y-axis guide rail, the dripping tube is located directly above the support base, one end of each peristaltic pump is connected to a corresponding dripping tube through a first connecting tube, and the other end is connected to the bioreactor through a second connecting tube.

2. The cell reaction solution sampling device according to claim 1, characterized in that: A waste liquid collecting tank is provided on one side of the support seat.

3. The cell reaction solution sampling device according to claim 2, characterized in that: The support seat is provided with a fixing groove, and the fixing groove is used to fix the orifice plate culture dish. There is a gap between the fixing groove and the waste liquid collection groove.

4. The cell reaction solution sampling device according to claim 1, characterized in that: The cell reaction liquid sampling device also includes a three-way valve and a clamp valve, wherein the first port of the three-way valve is connected to the peristaltic pump, the second port of the three-way valve is connected to the second connecting pipe, and the third port of the three-way valve is connected to the clamp valve.

5. The cell reaction solution sampling device according to claim 4, characterized in that: The cell reaction liquid sampling device further comprises a second liquid sensor and a first liquid sensor, wherein the second liquid sensor is arranged between the three-way valve and the peristaltic pump, and the first liquid sensor is arranged between the peristaltic pump and the dropper.

6. The cell reaction liquid sampling device according to claim 4 or 5, characterized in that: The pinch valve is connected to a compressed air machine.

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