EP pipe magnetic separator

Abstract

The utility model belongs to the field of biological experiment equipment, and particularly discloses an EP pipe magnetic separator. The EP pipe magnetic separator comprises a shell, the shell is provided with a plurality of installation parts, a plurality of longitudinal arc-shaped grooves are formed in the side walls of the installation parts, arc-shaped rubidium magnets are distributed in the arc-shaped grooves, and the outer arc surfaces of the arc-shaped rubidium magnets are connected with the inner walls of the arc-shaped grooves. A more uniform magnetic field can be provided for the position where the EP pipe is located, the phenomenon of agglomeration in the adsorption process of the magnetic microbeads is not prone to occurring, and separation can be better carried out. And meanwhile, a fixing piece is provided for the EP pipe, so that the EP pipe can be more stably arranged on the separator.

Description

Technical Field

[0001] The utility model belongs to the field of biological experimental equipment, in particular to an EP tube magnetic separator. Background Art

[0002] The existing 1.5ml / 2mlEP tube magnetic separators do not have a good retention design and are not tightly fitted with the 1.5ml / 2mlEP tubes. They need to be re-adsorbed after being accidentally detached. In addition, the existing 1.5ml / 2mlEP tube magnetic separators are mostly in the shape of long strips or cylinders, which are unstable on the table and prone to falling. In addition, the existing 1.5ml / 2ml magnetic racks are mostly round and square magnets located on the side of the magnetic rack. The magnetic microspheres gather together during the adsorption process. When removing the supernatant, the magnetic microspheres drop as the liquid level drops, which also leads to a large loss during the experiment. Utility Model Content

[0003] In order to solve the above problems, the utility model provides the following solutions:

[0004] EP tube magnetic separator, including

[0005] The shell has a plurality of mounting parts, the side walls of the mounting parts have a plurality of longitudinal arc grooves, the arc grooves are provided with arc-shaped nephelium magnets, and the outer arc surface of the arc-shaped nephelium magnets is connected to the inner wall of the arc groove.

[0006] In some embodiments, the arc-shaped groove has an elastic fixing member outside, and the two ends of any elastic fixing member are respectively connected to the edges of the arc-shaped groove; in any arc-shaped groove body: the EP tube is placed between the elastic fixing member and the arc-shaped rubidium magnet.

[0007] In some embodiments, the side surface of the shell is concave to form a mounting portion, and the arc-shaped groove is located on the inner concave wall of the mounting portion. The arc-shaped groove is located on the inner concave wall of the mounting portion.

[0008] In some embodiments, any arc-shaped groove is provided with a plurality of elastic fixing members from top to bottom.

[0009] In some embodiments, the arc-shaped neodymium magnet is disposed in contact with the inner wall of the arc-shaped groove.

[0010] In some embodiments, the fixed inner diameter of the elastic fixing member is smaller than the outer diameter of the 1.5 ml EP tube.

[0011] In some embodiments, it also includes a base, whose support column passes through the mounting hole of the shell; a threaded cap is provided after the support column of the base passes through the mounting hole of the shell; and the shell can rotate on the support column.

[0012] In some embodiments, the shell is in the shape of a triangular prism, and the mounting portion is located on a side wall of the shell.

[0013] In some embodiments, any side wall of the housing has a mounting portion.

[0014] In some embodiments, the arc-shaped neodymium magnet is a bar-shaped structure.

[0015] In some embodiments, the shell is made of polyethylene.

[0016] The present invention can provide a more uniform magnetic field to the location of the EP tube, which makes it less likely for the magnetic microbeads to aggregate during the adsorption process, and can better separate. At the same time, a fixing piece is provided for the EP tube, so that the EP tube can be more stably placed in the separator. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a top view of the utility model;

[0018] Figure 2 This is the AA cross-sectional view of the utility model;

[0019] Figure 3 This is the removal rate chart in Example 1;

[0020] Figure 4 This is a graph showing the CBA test results of the magnetic separator in Example 2.

[0021] In the figure:

[0022] 1 elastic fixing part, 2 arc-shaped neodymium magnet, 3 threaded cap, 4 mounting part, 5 supporting column, 6 base. DETAILED DESCRIPTION

[0023] The utility model is described in detail below in conjunction with the accompanying drawings.

[0024] EP tube magnetic separator, including

[0025] The housing has a plurality of mounting portions 4, the side walls of the mounting portions 4 have a plurality of longitudinal arc-shaped grooves, the arc-shaped neodymium magnets 2 are arranged in the arc-shaped grooves, and the outer arc surface of the arc-shaped neodymium magnets 2 is connected to the inner wall of the arc-shaped groove;

[0026] A base 6, whose support column 5 passes through the mounting hole of the shell;

[0027] Among them, there is an elastic fixing part 1 outside the arc-shaped groove, and the two ends of any elastic fixing part 1 are respectively connected to the edges of the two sides of the arc-shaped groove; in any arc-shaped groove body: the EP tube is placed between the elastic fixing part 1 and the arc-shaped rubidium magnet 2.

[0028] like Figure 1As shown in the figure, the side of the shell is concave to form a mounting portion 4, and the arc-shaped groove is located in the inner concave wall of the mounting portion 4. The arc-shaped groove is located in the inner concave wall of the mounting portion 4. Through this design, the mounting portion 4 forms a groove, and then when in use, the EP tube can be located in the mounting portion 4 as a whole, and the outer side wall of the EP tube does not protrude from the port of the mounting portion 4, which plays a comprehensive protection role. In order to further fix the EP tube, any arc-shaped groove is provided with a plurality of elastic fixing members 1 from top to bottom. The elastic fixing member 1 is elastic, and when the EP tube is placed, the elastic fixing member 1 can tighten it.

[0029] Combination Figure 2 As shown in the figure, the arc-shaped neodymium magnet 2 is arranged in contact with the inner wall of the arc-shaped groove, that is, the outer arc surface of the arc-shaped neodymium magnet 2 is in contact with the inner arc surface of the arc-shaped groove, so that the installation of the arc-shaped neodymium magnet 2 is more stable and convenient for installation. The two ends of the arc-shaped neodymium magnet 2 are respectively connected to the two edges of the arc-shaped groove.

[0030] The fixed inner diameter of the elastic fixing member 1 is smaller than the outer diameter of the 1.5ml EP tube, so the 1.5ml EP tube can be effectively fixed.

[0031] After the support column 5 of the base 6 passes through the mounting hole of the shell, a threaded cap 3 is provided. The main purpose of providing the threaded cap 3 is to prevent the shell from falling off the support column 5. The bottom plate of the base 6 can also adopt a triangular design, which not only ensures stability but also matches the triangular prism. The shell can rotate on the support column 5 to facilitate experimental operation.

[0032] like Figure 1 In the embodiment, the housing is in the shape of a triangular prism (not considering the bend), and the mounting portion 4 is located on the side wall of the housing. Any side wall of the housing has the mounting portion 4.

[0033] The arc-shaped neodymium magnet 2 is a strip-shaped structure with sufficient length to operate on EP tubes with different drug loads.

[0034] The shell is made of polyethylene.

[0035] The utility model is applied to magnetic bead sorting.

[0036] Embodiment 1:

[0037] Use this magnetic stand to remove CD8+T cells

[0038] (1) Ten C57 / 6 mice aged 6-8 weeks were selected;

[0039] (2) peripheral blood was collected from the orbit into EDTA anticoagulant tubes;

[0040] (3) mixing the collected peripheral blood;

[0041] (4) Add 5 times the volume of red lysis solution (BD: 647823);

[0042] (5) Incubate in dark for 15 min, centrifuge at 500 g for 5 min, and remove the supernatant;

[0043] (6) Add 1 ml of PBS, centrifuge at 500 g for 5 min, and remove the supernatant;

[0044] (7) Repeat step 6 once;

[0045] (8) Add 1 ml of PBS and resuspend the cells;

[0046] (9) Take 30 new EP tubes and add 100 μl of cell suspension to each tube;

[0047] (10) Take 20 tubes and add 5 μl of CD8+ removal reagent (Miltenyi Biotec: 130-104-075) and incubate in dark for 15 min;

[0048] (11) Transfer the EP tube to the magnetic stand and the conventional magnetic stand and let it stand for 1 min. Use a pipette to absorb the upper

[0049] Clear and transfer to a new EP tube;

[0050] (12) Repeat step (11) once;

[0051] (13) Centrifuge at 500 g for 5 min and discard the supernatant;

[0052] (14) Add 100 μl PBS to the EP tube;

[0053] (15) Add 2ul FITC Rat Anti-Mouse CD8a antibody (BD: 561966) to each tube;

[0054] (16) Incubate in dark for 15 min. After incubation, add 1 ml of PBS to each tube and centrifuge at 500 g for 5 min.

[0055] (17) Remove the supernatant and add 1 ml of PBS to wash once;

[0056] (18) Resuspend each tube with 200ul PBS and add 50ul absolute counting microspheres (Themo:

[0057] C36995)

[0058] (19) The flow cytometer (Longcyte) was used, and the absolute count of microspheres was set as 2000.

[0059] (20) The number of CD8a+ in 20 samples was counted, and the data are shown in Table 1

[0060] Table 1 Comparison of the removal of C8+ cells using the magnetic separator of the present invention (Group N was not removed, Group Q used the magnetic frame of the present invention to remove CD8+ cells, and Group C used a conventional separator)

[0061]

[0062] The magnetic separator of the present invention can be used as a magnetic separator to perform magnetic bead separation experiments, and the removal rate is as high as 99.77%.

[0063] Using the patented invention to perform multi-factor CBA testing

[0064] (1) Add 25ul of premixed capture microspheres (40,000 particles / ml / weight) to each tube;

[0065] (2) Add 25ul of premixed protein solution diluted in multiple proportions according to the number, namely 10000pg / ml, 2500pg / ml, 625pg / ml, 156.25pg / ml, 39pg / ml, 9.76pg / ml, 2.44pg / ml, and 0pg / ml;

[0066] (3) Add 25ul of matrix solution to each tube;

[0067] (4) Add 25ul of premixed detection antibody solution to each tube;

[0068] (5) Place the plate in a 96-well plate mixer at 850 rpm and incubate in the dark for 2 h;

[0069] (6) After the incubation, add 1 ml of PBST, place on the magnetic separator of the present invention, and let stand for 1 min;

[0070] (7) Remove the supernatant with a pipette, add 1 ml of PBST, place on the magnetic separator of the present invention, and let stand for 1 min;

[0071] (8) Add 50ul1ug / ml SA-PE (themo: 12-4317-87) to each tube

[0072] (9) Place the plate in a 96-well plate mixer at 850 rpm and incubate in the dark for 30 min;

[0073] (10) After the incubation, add 1 ml of PBST, place on the magnetic separator of the present invention, and let stand for 1 min;

[0074] (11) Remove the supernatant with a pipette, add 1 ml of PBST, place on the magnetic separator of the present invention, and let stand for 1 min;

[0075] (12) Remove the supernatant with a pipette, add 200ul PBST, set the flow cytometer to quantitatively aspirate 30ul, and load the flow cytometer (longcyte).

[0076] Using the magnetic separator of the present invention to perform CBA detection, the magnetic microspheres have an excellent recovery rate as shown in Table 2;

[0077] Table 2 Recovery rate of captured microspheres by various factors

[0078] name Theoretical value Actual value Recovery rate IL-1β microspheres 150 139 92.67％ IL-4 microspheres 150 136 90.67％ IL-5 microspheres 150 144 96.00％ IL-6 microspheres 150 141 94.00％

[0079] The magnetic separator of the present invention is used to perform CBA detection, which meets the experimental requirements, and the results show a good linear relationship. Figure 2 .

[0080] It is clear to those skilled in the art that various modifications to the above embodiments can be made without departing from the overall spirit and concept of the present invention. All of them fall within the protection scope of the present invention. The protection scheme of the present invention shall be subject to the claims attached to the present invention.

Claims

1. EP tube magnetic separator, characterized in that: include A housing having a plurality of mounting portions (4), the side walls of the mounting portions (4) having a plurality of longitudinal arc-shaped grooves, arc-shaped nephelium magnets (2) being arranged in the arc-shaped grooves, and the outer arc surface of the arc-shaped nephelium magnets (2) being connected to the inner wall of the arc-shaped grooves; The base (6) has a support column (5) passing through the mounting hole of the shell.

2. The EP tube magnetic separator according to claim 1, characterized in that: An elastic fixing member (1) is provided outside the arc-shaped groove, and the two ends of any elastic fixing member (1) are respectively connected to the edges on both sides of the arc-shaped groove; in any arc-shaped groove body: an EP tube placement position is provided between the elastic fixing member (1) and the arc-shaped rubidium magnet (2).

3. The EP tube magnetic separator according to claim 1, characterized in that: The side surface of the shell is concave to form a mounting portion (4), and the arc-shaped groove is located on the inner concave wall of the mounting portion (4).

4. The EP tube magnetic separator according to claim 2, characterized in that: Any arc-shaped groove is provided with a plurality of elastic fixing members (1) from top to bottom.

5. The EP tube magnetic separator according to claim 2, characterized in that: The arc-shaped neodymium magnet (2) is arranged to fit the inner wall of the arc-shaped groove.

6. The EP tube magnetic separator according to claim 2, characterized in that: The fixed inner diameter of the elastic fixing member (1) is smaller than the outer diameter of the 1.5 ml EP tube.

7. The EP tube magnetic separator according to claim 1, characterized in that: A threaded cap (3) is arranged after the support column (5) of the base (6) passes through the mounting hole of the shell; the shell can rotate on the support column (5).

8. The EP tube magnetic separator according to claim 1, characterized in that: The shell is in the shape of a triangular prism, and the mounting portion (4) is located on the side wall of the shell.

9. The EP tube magnetic separator according to claim 8, characterized in that: Any side wall of the shell has a mounting portion (4).

10. The EP tube magnetic separator according to claim 1, characterized in that: The arc-shaped neodymium magnet (2) is a strip-shaped structure; and the shell is made of polyethylene.

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