Cylindrical embedding mold suitable for frozen section of brain tissue of rodent

Abstract

The utility model relates to the technical field of basic medical experiments, and provides a cylindrical embedding mold suitable for frozen sections of brain tissues of rodents. Comprising a cylindrical mold, the cylindrical mold is provided with a mold bottom plate, and the whole cylindrical mold is made of aluminum-foil paper; a splitting stay wire is arranged on the inner side of an arc-shaped side plate of the cylindrical mold in an attached mode, and the whole splitting stay wire is in a closed shape. Wherein the left pull wire, the bottom pull wire, the right pull wire and the lifting U-shaped wire of the splitting pull wire are sequentially connected end to end; and a certain distance is formed between the bottom stay wire and the mold bottom plate. The cylindrical embedding mold has the beneficial effects that the aluminum-foil paper can be easily torn to take out the tissue embedding block by pulling the splitting pull line (easy-to-tear line) in the mold towards the lower part of the outer side, and if the tissue embedding block is still remained after being sliced and needs to be stored, the torn aluminum-foil paper of the original mold can be used for wrapping the tissue embedding block, so that the tissue embedding block is convenient to store again.

Description

Technical Field

[0001] The utility model relates to the technical field of basic medical experiments, in particular to a cylindrical embedding mold suitable for frozen sections of rodent brain tissue. Background Art

[0002] In the frozen section experiment, the dehydrated and fixed tissue is embedded in OCT glue. After the glue is quickly frozen into a solid, it is placed on a freezing microtome and continuously cut into tissue sections of different thicknesses according to the experimental requirements for subsequent staining experiments. The presentation of complete tissue sections requires high tissue embedding. Generally, embedding molds are used for embedding. OCT glue is poured into the mold. After the bubbles in the glue are naturally discharged, the tissue is placed vertically, and then the embedding mold is placed in liquid nitrogen or a -80℃ refrigerator for quick freezing. After the OCT glue solidifies, it is taken out, marked, and stored in a -20℃ refrigerator.

[0003] After the tissue block is embedded in the stainless steel mold, it is difficult to remove the tissue block from the mold. It needs to be placed at room temperature for a period of time or soaked in warm water to remove the embedded block, which can easily cause the OCT glue to melt. Use a marker to mark the sample name on the mold after embedding. After freezing at -80℃, the mark is easy to disappear due to the PVC and stainless steel materials, which is not conducive to distinguishing the samples later.

[0004] In view of this, the present utility model is proposed. Utility Model Content

[0005] The utility model aims to provide a cylindrical embedding mold suitable for frozen sections of rodent brain tissue, so as to solve the technical problems existing in the prior art.

[0006] To achieve the above object, the technical solution adopted by the utility model is: a cylindrical embedding mold suitable for frozen sections of rodent brain tissue, comprising: a cylindrical mold, the cylindrical mold having a mold bottom plate, the cylindrical mold being made of aluminum foil as a whole;

[0007] A split pull line is fitted on the inner side of the arc-shaped side plate of the cylindrical mold, and the split pull line is closed as a whole; wherein the left pull line, the bottom pull line, the right pull line and the U-shaped pull line of the split pull line are connected end to end in sequence; the bottom pull line is at a certain distance from the bottom plate of the mold.

[0008] In an optional embodiment, label paper is pasted on the outer side of the arc-shaped side plate of the cylindrical mold.

[0009] In an optional embodiment, the diameters of the mold bottom plate and the arc-shaped side plates of the cylindrical mold are both 25 mm; and the height of the cylindrical mold is 35 mm.

[0010] In an optional embodiment, the left pull wire is parallel to the right pull wire, and the distance between the left pull wire and the right pull wire is 5mm-8mm.

[0011] In an optional embodiment, the distance between the bottom pull wire and the mold bottom plate is greater than 5 mm.

[0012] In an optional embodiment, the pulling U-shaped line extends out of the top of the curved side plate of the cylindrical mold.

[0013] The beneficial effect of the utility model is that the cylindrical embedding mold can be easily torn open by pulling the splitting line (easy-tear line) inside the mold to the outside and below, and the aluminum foil can be easily torn open to take out the tissue embedding block. If there is still some tissue embedding block left after slicing to be stored, the torn original aluminum foil of the mold can be used to wrap the tissue embedding block for easy storage again. In addition, a label paper is attached to the outside of the mold for sample identification, and the label is firm and does not disappear due to too low storage temperature. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0015] Figure 1 The present invention is a schematic structural diagram of a cylindrical embedding mold suitable for frozen sections of rodent brain tissue provided in one embodiment of the present invention.

[0016] Among them, the figure markings are: 1-cylindrical mold, 2-label paper, 3-split pull line, 31-left pull line, 32-bottom pull line, 33-right pull line, 34-lifting U-shaped pull line. DETAILED DESCRIPTION

[0017] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0018] It should be noted that when a component is referred to as being "fixed on" or "disposed on" another component, it may be directly or indirectly located on the other component. When a component is referred to as being "connected to" another component, it may be directly or indirectly connected to the other component. The directions or positions indicated by the terms "upper", "lower", "left", "right", "front", "back", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc. are based on the directions or positions shown in the accompanying drawings and are only for the convenience of description and cannot be understood as limitations on the present technical solution. The terms "first" and "second" are only used for the convenience of description and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features. "Multiple" means two or more, unless otherwise clearly and specifically defined.

[0019] Embodiment 1

[0020] Please see attached Figure 1 The purpose of this embodiment is to provide a cylindrical embedding mold suitable for frozen sections of rodent brain tissue, including: a cylindrical mold 1, the cylindrical mold 1 has a mold bottom plate, and the cylindrical mold 1 is made of aluminum foil as a whole. Aluminum foil, as a metal, has good temperature tolerance and is not prone to wrinkling and deformation. The diameter of the mold bottom plate and the arc-shaped side plate of the cylindrical mold 1 are both 25 mm; the height of the cylindrical mold 1 is 35 mm.

[0021] Specifically, the inner side of the curved side panel of the cylindrical mold 1 is fitted with a splitting wire 3, and the splitting wire 3 is in a closed shape as a whole; wherein the left wire 31, the bottom wire 32, the right wire 33 and the lifting U-shaped wire 34 of the splitting wire 3 are connected end to end in sequence; the bottom wire 32 is at a certain distance from the mold bottom plate. In this embodiment, the distance between the bottom wire 32 and the mold bottom plate is 8 mm. After the aluminum foil between the left wire 31 and the right wire 33 is torn off, a part of the aluminum foil is still retained on the upper part of the mold bottom plate. The remaining tissue blocks after slicing can still be wrapped with the aluminum foil with the sample mark after tearing, which is convenient for storage. The aluminum foil retained on the mold bottom plate can ensure that the bottom of the remaining tissue blocks is not exposed. It should be pointed out that the lifting U-shaped wire 34 extends out of the top of the curved side panel of the cylindrical mold 1, providing space for the operator to insert his fingers, which is convenient for operation. The left pull line 31 is parallel to the right pull line 33, and the distance between the left pull line 31 and the right pull line 33 is 5 mm. The operator can easily tear open the aluminum foil to take out the tissue embedding block by pulling the split pull line 3 inside the mold downward and outward.

[0022] Specifically, a label paper 2 is pasted on the outer side of the arc-shaped side plate of the cylindrical mold 1. The operator uses a common laboratory marker pen or a marker pen to mark the label paper 2, and the mark is not easy to be lost, which is conducive to sample identification.

[0023] Embodiment 2

[0024] Please see attached Figure 1 The purpose of this embodiment is to provide a cylindrical embedding mold suitable for frozen sections of rodent brain tissue, including: a cylindrical mold 1, the cylindrical mold 1 has a mold bottom plate, and the cylindrical mold 1 is made of aluminum foil as a whole. Aluminum foil, as a metal, has good temperature tolerance and is not prone to wrinkling and deformation. The diameter of the mold bottom plate and the arc-shaped side plate of the cylindrical mold 1 are both 25 mm; the height of the cylindrical mold 1 is 35 mm.

[0025] Specifically, the inner side of the curved side panel of the cylindrical mold 1 is fitted with a splitting wire 3, and the splitting wire 3 is in a closed shape as a whole; wherein the left wire 31, the bottom wire 32, the right wire 33 and the lifting U-shaped wire 34 of the splitting wire 3 are connected end to end in sequence; the bottom wire 32 is at a certain distance from the mold bottom plate. In this embodiment, the distance between the bottom wire 32 and the mold bottom plate is 6 mm. After the aluminum foil between the left wire 31 and the right wire 33 is torn off, a part of the aluminum foil is still retained on the upper part of the mold bottom plate. The remaining tissue blocks after slicing can still be wrapped with the aluminum foil with the sample mark after tearing, which is convenient for storage. The aluminum foil retained on the mold bottom plate can ensure that the bottom of the remaining tissue blocks is not exposed. It should be pointed out that the lifting U-shaped wire 34 extends out of the top of the curved side panel of the cylindrical mold 1, providing space for the operator to insert his fingers, which is convenient for operation. The left pull line 31 is parallel to the right pull line 33, and the distance between the left pull line 31 and the right pull line 33 is 8 mm. The operator can easily tear open the aluminum foil to take out the tissue embedding block by pulling the split pull line 3 inside the mold downward and outward.

[0026] Specifically, a label paper 2 is pasted on the outer side of the arc-shaped side plate of the cylindrical mold 1. The operator uses a common laboratory marker pen or a marker pen to mark the label paper 2, and the mark is not easy to be lost, which is conducive to sample identification.

[0027] Any modifications, equivalent substitutions and improvements made within the spirit and principles of this utility model should be included in the protection scope of this utility model.

Claims

1. A cylindrical embedding mold suitable for frozen sections of rodent brain tissue, characterized in that: include: A cylindrical mold (1), wherein the cylindrical mold (1) has a mold bottom plate, and the entire cylindrical mold (1) is made of aluminum foil; A splitting pull line (3) is fitted on the inner side of the arc-shaped side plate of the cylindrical mold (1), and the splitting pull line (3) is in a closed shape as a whole; wherein the left pull line (31), the bottom pull line (32), the right pull line (33) and the lifting U-shaped line (34) of the splitting pull line (3) are connected end to end in sequence; and the bottom pull line (32) is at a certain distance from the bottom plate of the mold.

2. The cylindrical embedding mold suitable for frozen sections of rodent brain tissue according to claim 1, characterized in that: Label paper (2) is pasted on the outer side of the arc-shaped side plate of the cylindrical mold (1).

3. The cylindrical embedding mold suitable for frozen sections of rodent brain tissue according to claim 1, characterized in that: The diameters of the mold bottom plate and the arc-shaped side plates of the cylindrical mold (1) are both 25 mm; the height of the cylindrical mold (1) is 35 mm.

4. The cylindrical embedding mold suitable for frozen sections of rodent brain tissue according to claim 1, characterized in that: The left pull wire (31) is parallel to the right pull wire (33), and the distance between the left pull wire (31) and the right pull wire (33) is 5mm-8mm.

5. The cylindrical embedding mold suitable for frozen sections of rodent brain tissue according to claim 1, characterized in that: The distance between the bottom pull wire (32) and the mold bottom plate is greater than 5 mm.

6. The cylindrical embedding mold suitable for frozen sections of rodent brain tissue according to claim 1, characterized in that: The lifting U-shaped line (34) extends out of the top of the arc-shaped side plate of the cylindrical mold (1).

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