Liquid changing device for cell factory

Abstract

The utility model discloses a liquid changing device for a cell factory. The liquid changing device comprises a workbench, a peristaltic pump, an inclined device, the cell factory, a first connector, a large-opening gas conversion suite, a second connector, a large-opening three-way suite, a hose clamp, a joint, a hose, a waste liquid barrel and a new liquid barrel. The inclined devices are mounted on the surface of the worktable, so that the cell factories can be conveniently clamped, two-time inclination of the cell factories is controlled, waste liquid in the cell factories can be conveniently and quickly discharged, and the plurality of cell factories can be conveniently driven to synchronously incline and quickly discharge the waste liquid through the mounting of the plurality of inclined devices; the liquid changing speed of the cell factory is increased, then the liquid changing efficiency of the cell factory is improved, the clamping piece is arranged on the inner side of the movable seat, the cell factory can be conveniently clamped and fixed, the cell factory can be synchronously inclined along with the movable seat and the supporting plate, and the stability of the cell factory during inclination is guaranteed.

Description

Technical Field

[0001] The utility model relates to the field related to cell factories, in particular to a liquid replacement device for cell factories. Background Art

[0002] Cell culture medium replacement is a common routine operation in the process of cell culture. It replaces the metabolic waste products of cells during the growth process so that cells can grow normally and prevent cells from dying during long-term growth.

[0003] The Chinese patent with patent number CN202022500509.6 discloses "A liquid exchange system for a cell factory", which uses liquid pipes for gas exchange, so that the gas exchange resistance of the cell factory is smaller when the liquid is inlet or outlet, and the pressure inside the cell factory during operation is reduced, which can greatly speed up the liquid inlet and outlet speed, improve work efficiency, and reduce pollution; at the same time, the cell factory has no gas exchange with the external environment, which can ensure that the personnel and environment during biological positive operation will not be polluted;

[0004] This structure has certain advantages, but still has some shortcomings:

[0005] When the fluid exchange system is used for fluid exchange, the cell factory usually needs to be tilted twice so that the multiple layers of internal liquid can be concentrated in one place for full discharge, and then the liquid can be replaced. When multiple cell factories are connected in series and the fluid needs to be replaced at the same time, the single cell factory can only be tilted and drained in sequence, and the clamping state of multiple hose clamps needs to be constantly adjusted at this time. The overall fluid exchange efficiency is low and needs to be improved. Utility Model Content

[0006] Therefore, in order to solve the above-mentioned deficiencies, the utility model provides a liquid replacement device for a cell factory.

[0007] In order to achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a liquid exchange device for a cell factory, comprising a workbench, a peristaltic pump, a tilting device, a cell factory, a first connector, a large-mouth gas conversion kit, a second connector, a large-mouth three-way kit, a hose clamp, a joint, a hose, a waste liquid barrel, and a new liquid barrel. A peristaltic pump is placed on the upper end of the workbench. The cell factory is used for cell culture. The front end of the cell factory is respectively provided with a first connector and a second connector. The front end of the first connector is installed with a large-mouth gas conversion kit, and the front end of the second connector is installed with a large-mouth three-way kit. The hose clamp is arranged on the outside of the large-mouth three-way kit. The right end of the large-mouth three-way kit is installed with a joint, and its left end is connected with a hose. The hose can be connected to the waste liquid barrel and the new liquid barrel respectively. The middle part of the hose is installed inside the peristaltic pump, the tilting device is installed on the top of the workbench, and a cell factory is clamped on its inner side, the tilting device includes a base, a movable seat, a mounting plate, a bracket, an electric cylinder, a second mounting plate, a second electric cylinder, a support rod, and a clamping member, the base is bolted to the top of the workbench, and a movable seat is hinged on its top, the mounting plate is bolted and fixed to the base, and a bracket is fixed on its top, the bottom end of the electric cylinder is rotatably connected to the bracket, and the top end of the electric cylinder is rotatably connected to the movable seat, the second mounting plate is bolted to the movable seat, and a second electric cylinder is fixed on its top, the top of the second electric cylinder is fixed with a support rod, the support rod is in contact with the cell factory, the right end of the clamping member is rotatably connected to the movable seat, and the left end of the clamping member is in contact with the cell factory.

[0008] As a further solution of the utility model, the clamping member includes a support plate, a rotating shaft, a strong spring, a clamping plate, and a rubber pad. A rotating shaft is fixed to the left end of the support plate, and the rotating shaft is rotatably connected to the movable seat. A strong spring is fixed to the right end of the support plate, and the left end of the clamping plate is elastically connected to the support plate by a strong spring.

[0009] As a further solution of the utility model, a rubber pad is fixed to the right end of the clamping plate to strengthen the tightness of the connection between the clamping plate and the cell factory, making it easier for the clamping plate to clamp the cell factory and drive the cell factory to rotate synchronously, thereby tilting the cell factory forward and tilting the multi-layer cell fluid to the front end for easy discharge.

[0010] As a further solution of the utility model, a groove is provided at the front end of the movable seat, so that when the cell factory rotates forward driven by the clamping member, the groove provides downward movement space for the forward tilt of the cell factory, thereby realizing smooth forward tilt of the cell factory.

[0011] As a further solution of the utility model, the clamping members are provided with two groups, which are symmetrically distributed at the left and right ends of the movable seat, so as to facilitate left and right clamping of the cell factory, realize stable placement of the cell factory, and facilitate the cell factory to tilt to the right and forward under the drive of the tilting device, thereby discharging the multi-layer culture medium inside the cell factory.

[0012] As a further solution of the utility model, the cross section of the clamping plate is "T"-shaped, which facilitates the horizontal movement of the clamping member inside the support plate and facilitates the stable movement and efficient clamping of the cell factory.

[0013] As a further solution of the utility model, a rectangular through hole is provided in the middle of the movable seat, and a support rod is movable in the rectangular through hole. The setting of the rectangular through hole facilitates the vertical upward movement of the support rod, thereby supporting the cell factory to rotate with the rotating shaft as the fulcrum, thereby realizing the forward tilting movement of the cell factory.

[0014] As a further solution of the utility model, the support rod is made of stainless steel, is not easy to deform, and is durable.

[0015] As a further solution of the utility model, the strong spring is made of alloy spring steel, has excellent elastic restoring force, can achieve a stable clamping effect, and is convenient for stable clamping of the cell factory.

[0016] Compared with the prior art, the present invention provides a liquid replacement device for a cell factory, which has the following features:

[0017] Beneficial effects:

[0018] 1. In the utility model, a tilting device is installed on the surface of the workbench to facilitate clamping of the cell factory, thereby controlling the two tilts of the cell factory, facilitating the rapid discharge of waste liquid in the cell factory. By installing multiple tilting devices, multiple cell factories are connected in series, which facilitates driving multiple cell factories to tilt synchronously and discharge waste liquid quickly, thereby increasing the liquid replacement speed of the cell factory and further improving the liquid replacement efficiency of the cell factory.

[0019] 2. In the utility model, a clamping piece is arranged on the inner side of the movable seat, so as to facilitate clamping and fixing of the cell factory, and facilitate synchronous tilting of the cell factory with the movable seat and the support plate, thereby ensuring the stability of the cell factory when tilted. The clamping piece has a simple structure and flexible operation, and is convenient for realizing a quick connection between the cell factory and the tilting device, thereby facilitating quick removal or installation of the cell factory from the tilting device, thereby improving the convenience of operation. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a three-dimensional structural schematic diagram of the utility model;

[0021] Figure 2 This is a schematic diagram of the three-dimensional structure of the tilting device of the utility model;

[0022] Figure 3 It is a schematic diagram of the cross-sectional structure of the tilting device of the utility model;

[0023] Figure 4 This is a schematic diagram of the three-dimensional structure of the clamping member of the utility model;

[0024] Figure 5 It is a schematic diagram of the cross-sectional structure of the clamping member of the utility model.

[0025] Including: workbench-1, peristaltic pump-2, tilting device-3, cell factory-4, first connector-5, large-mouth gas conversion kit-6, second connector-7, large-mouth three-way kit-8, hose clamp-9, connector-10, hose-11, waste liquid barrel-12, new liquid barrel-13, base-31, movable seat-32, mounting plate-33, bracket-34, electric cylinder-35, second mounting plate-36, second electric cylinder-37, support rod-38, clamping piece-39, support plate-391, rotating shaft-392, strong spring-393, clamping plate-394, rubber pad-395. DETAILED DESCRIPTION

[0026] In order to further explain the technical solution of the present utility model, it is described in detail through specific embodiments below.

[0027] See also Figure 1 In the present utility model embodiment:

[0028] A liquid exchange device for a cell factory comprises a workbench 1, a peristaltic pump 2, a cell factory 4, a first connector 5, a large-mouth gas conversion kit 6, a second connector 7, a large-mouth three-way kit 8, a hose clamp 9, a joint 10, a hose 11, a waste liquid barrel 12, and a new liquid barrel 13. The peristaltic pump 2 is placed on the upper end of the workbench 1. The cell factory 4 is used for cell culture. The front end of the cell factory 4 is respectively provided with a first connector 5 and a second connector 7. The front end of the first connector 5 is installed with a large-mouth gas conversion kit 6, and the front end of the second connector 7 is installed with a large-mouth three-way kit 8. The hose clamp 9 is arranged on the outside of the large-mouth three-way kit 8. The right end of the large-mouth three-way kit 8 is installed with a joint 10, and the left end thereof is connected with a hose 11. The hose 11 can be connected with the waste liquid barrel 12 and the new liquid barrel 13 respectively, and the middle part of the hose 11 is installed inside the peristaltic pump 2.

[0029] See also Figure 2-3 , in the embodiment of the utility model:

[0030] The tilting device 3 is installed on the top of the workbench 1, and the cell factory 4 is clamped inside the tilting device 3. The tilting device 3 includes a base 31, a movable seat 32, a mounting plate 33, a bracket 34, an electric cylinder 35, a second mounting plate 36, a second electric cylinder 37, a support rod 38, and a clamping member 39. The base 31 is bolted to the top of the workbench 1, and the movable seat 32 is hinged on the top of the base 31. The mounting plate 33 is bolted and fixed to the base 31, and the bracket 34 is fixed on the top of the base 31. The bottom end of the electric cylinder 35 is rotatably connected to the bracket 34. The top of the movable seat 32 is rotatably connected, the second mounting plate 36 is bolted to the movable seat 32, and the top of the movable seat 32 is fixed with a second electric cylinder 37, the top of the second electric cylinder 37 is fixed with a support rod 38, the support rod 38 is in contact with the cell factory 4, the right end of the clamping member 39 is rotatably connected to the movable seat 32, and the left end of the clamping member 39 is in contact with the cell factory 4, and the front end of the movable seat 32 is provided with a groove, so that when the cell factory 4 rotates forward under the drive of the clamping member 39, the groove provides downward movement for the forward tilt of the cell factory 4 The movable space realizes the smooth forward tilting of the cell factory 4. Two groups of clamping members 39 are arranged, which are symmetrically distributed at the left and right ends of the movable seat 32, so as to clamp the cell factory 4 on the left and right sides, realize the stable placement of the cell factory 4, and facilitate the cell factory 4 to tilt to the right and tilt forward under the drive of the tilting device 3, so as to discharge the multi-layer culture fluid inside the cell factory 4. Moreover, through the setting of multiple tilting devices 3, it is convenient to synchronously tilt the multiple clamped cell factories 4 to discharge the internal waste liquid, realize rapid liquid replacement, and speed up the liquid replacement speed and efficiency. A rectangular through hole is arranged in the middle of the movable seat 32, and a support rod 38 is movable in the rectangular through hole. Through the setting of the rectangular through hole, the support rod 38 is convenient to move vertically upward, and then support the cell factory 4 to rotate with the rotating shaft 392 as the fulcrum, so as to realize the forward tilting activity of the cell factory 4. When tilting forward, the contact point between the cell factory 4 and the support rod 38 changes with the tilting of the cell factory 4, so as to adapt to the forward tilting degree of the cell factory 4.

[0031] See also Figure 4-5 , in the embodiment of the utility model:

[0032] The clamping member 39 includes a support plate 391, a rotating shaft 392, a strong spring 393, a clamping plate 394, and a rubber pad 395. The left end of the support plate 391 is fixed with a rotating shaft 392, and the rotating shaft 392 is rotatably connected to the movable seat 32. The right end of the support plate 391 is fixed with a strong spring 393. The left end of the clamping plate 394 is elastically connected to the support plate 391 by a strong spring 393, and the right end of the clamping plate 394 is fixed with a rubber pad 395 to strengthen the tightness of the connection between the clamping plate 394 and the cell factory 4, so that the clamping plate 394 can clamp the cell factory and drive the cell factory 4 to rotate synchronously, so as to realize the forward tilting of the cell factory and tilt the multilayer cell fluid to the front end for easy discharge. The cross section of the clamping plate 394 is in a "T" shape, so that the clamping member 394 can move horizontally inside the support plate 391, so as to facilitate the stable movement and efficient clamping of the cell factory 4.

[0033] refer to Figure 1-Figure 5 When in use, the cell factory 4 is embedded in the two groups of clamping members 39, and the cell factory 4 is clamped and fastened by the elastically connected clamping plates 394, so that the cell factory 4 is installed in the tilting device 3, and then the large-mouth gas conversion kit 6 is installed in the first connector 5, and the large-mouth three-way kit 8 is installed in the second connector 7, and the hose 11 is connected by the connector 10, and then the hose 11 is installed in the peristaltic pump 2, and the other end of the hose 11 is connected to the waste liquid bucket 12, wherein the two adjacent groups of large-mouth three-way kits 8 can be connected by the connector 10, so as to realize the series connection of multiple cell factories 4 and perform synchronous liquid replacement;

[0034] Then, by starting the peristaltic pump 2, the waste liquid in the cell factory 4 is extracted into the waste liquid barrel 12 through the wide-mouth three-way kit 8 and the hose 11, wherein the movable seat 32 is rotated by the push of the electric cylinder 35, driving the movable seat 32 and the cell factory 4 to tilt to the right, and the multi-layer cell liquid is concentrated at one end, and then the support rod 38 is pushed upward by the start of the second electric cylinder 37, thereby pushing the cell factory 4 to tilt forward with the rotating shaft 392 as the fulcrum. When tilting forward, the support plate 391, the clamping plate 394, and the cell factory 4 all tilt forward synchronously, and the cell liquid is collected at the front end for rapid drainage, wherein the flow and blocking state of the liquid can be adjusted by the clamping state of the hose clamp 9;

[0035] After the liquid in the cell factory 4 is drained, the hose 11 is connected to a new liquid barrel, and the cell factory 4 is reset from the inclined state to the horizontal state, and the new culture liquid is transferred to the cell factory 4 by starting the peristaltic pump 2. After the new culture liquid is replaced, the cell factory 4 is removed from the tilting device 3 and shaken to allow the new culture liquid to cover the wall of the cell factory 4, thereby achieving liquid replacement.

[0036] The control method of the present invention is to control by manually starting and closing the switch. The wiring diagram of the power element and the provision of power supply are common knowledge in the field, and the present invention is mainly used to protect mechanical devices, so the present invention will not explain the control method and wiring arrangement in detail.

[0037] The control method of the present invention is automatic control through a controller. The control circuit of the controller can be realized by simple programming by technicians in this field. The provision of power is also common knowledge in this field. The present invention is mainly used to protect mechanical devices, so the present invention will no longer explain the control method and circuit connection in detail.

[0038] The above description is only a preferred example of the present invention and is not intended to limit the present invention. Although the present invention is described in detail with reference to the above embodiments, those skilled in the art can still modify the technical solutions described in the above embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A liquid replacement device for a cell factory, comprising a workbench (1), a peristaltic pump (2), a cell factory (4), a first connector (5), a large-mouth gas conversion kit (6), a second connector (7), a large-mouth three-way kit (8), a hose clamp (9), a joint (10), a hose (11), a waste liquid bucket (12), and a new liquid bucket (13), wherein a peristaltic pump (2) is placed at the upper end of the workbench (1), and the cell factory (4) is used for cell culture; Features: The invention also comprises a tilting device (3), which is installed at the top of the workbench (1) and has a cell factory (4) clamped inside. The tilting device (3) comprises a base (31), a movable seat (32), a mounting plate (33), a bracket (34), an electric cylinder (35), a second mounting plate (36), a second electric cylinder (37), a support rod (38), and a clamping member (39). The base (31) is bolted to the top of the workbench (1), and has a movable seat (32) hinged on its top. The mounting plate (33) is bolted to the base (31). 1) is locked and fixed, and a bracket (34) is fixed on its top, the bottom end of the electric cylinder (35) is rotatably connected to the bracket (34), and the top end of the electric cylinder (35) is rotatably connected to the movable seat (32), the second mounting plate (36) is bolted to the movable seat (32), and a second electric cylinder (37) is fixed on its top, a support rod (38) is fixed on the top of the second electric cylinder (37), and the support rod (38) is in contact with the cell factory (4), the right end of the clamping member (39) is rotatably connected to the movable seat (32), and the left end of the clamping member (39) is in contact with the cell factory (4).

2. A liquid replacement device for a cell factory according to claim 1, characterized in that: The clamping member (39) comprises a support plate (391), a rotating shaft (392), a strong spring (393), a clamping plate (394), and a rubber pad (395); the left end of the support plate (391) is fixed with a rotating shaft (392); the rotating shaft (392) is rotatably connected to the movable seat (32); the right end of the support plate (391) is fixed with a strong spring (393); the left end of the clamping plate (394) is elastically connected to the support plate (391) by a strong spring (393).

3. A liquid replacement device for a cell factory according to claim 1, characterized in that: The front end of the cell factory (4) is respectively provided with a first connector (5) and a second connector (7); the front end of the first connector (5) is installed with a large-mouth gas conversion kit (6); the front end of the second connector (7) is installed with a large-mouth three-way kit (8); the hose clamp (9) is arranged on the outside of the large-mouth three-way kit (8); the right end of the large-mouth three-way kit (8) is installed with a joint (10), and the left end thereof is connected with a hose (11); the hose (11) can be connected with a waste liquid barrel (12) and a new liquid barrel (13) respectively; the middle part of the hose (11) is installed inside the peristaltic pump (2).

4. A liquid replacement device for a cell factory according to claim 2, characterized in that: A rubber pad (395) is fixed to the right end of the clamping plate (394).

5. The liquid replacement device of a cell factory according to claim 1, characterized in that: The front end of the movable seat (32) is provided with a groove.

6. The liquid replacement device of a cell factory according to claim 1, characterized in that: The clamping members (39) are provided in two groups and are symmetrically distributed at the left and right ends inside the movable seat (32).

7. The liquid replacement device of a cell factory according to claim 2, characterized in that: The cross section of the clamping plate (394) is in a "T" shape.

8. The liquid replacement device for a cell factory according to claim 1, characterized in that: A rectangular through hole is provided in the middle of the movable seat (32), and a support rod (38) is movable in the rectangular through hole.

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