Controllable controlled atmosphere rotating cavity solid fermentation device

Abstract

The utility model relates to the technical field of biological fermentation devices, in particular to a controllable air-conditioning rotating cavity solid fermentation device which comprises a motor, an air generating device and a double-cone cavity capable of rotating on an output shaft of the motor, the interior of the double-cone cavity is a cavity, and the inner wall of the double-cone cavity is provided with an inductive probe, a gas inlet and a spray inlet; the spraying inlet is connected with the spraying device; the gas generating device comprises a panel provided with a plurality of gas outlets, a gas filtering device, a gas mixing temperature control device, an air compressor, a pure oxygen generator and a central controller; one end of the gas outlet is connected with the gas inlet, the other end of the gas outlet is sequentially connected with the gas filtering device and the gas mixing temperature control device, and the gas mixing temperature control device is respectively connected with the air compressor, the pure oxygen generator and the central controller; and the central controller is also respectively connected with the inductive probe and the spraying device. The device is suitable for spore-producing solid fermentation of fungus biopesticide.

Description

Technical Field

[0001] The utility model relates to the technical field of biological fermentation devices, in particular to a rotating cavity solid fermentation device with controllable gas regulation. Background Art

[0002] Solid fermentation is a microbial fermentation carried out when the fermentation material is solid. For example, the solid fermentation equipment for compost fermentation disclosed in CN201820017134.8 is suitable for high-temperature microbial humification fermentation of anaerobic fermentation or facultative anaerobic fermentation of compost, but it is not suitable for solid spore fermentation of biological pesticides.

[0003] Solid spore fermentation of biopesticides is a fermentation process in which microorganisms grow on a solid matrix and then produce spores on the solid matrix. The characteristics of this process are that the microorganisms are aerobic during the growth period, generate a lot of heat, and prefer high humidity atmosphere conditions, while the spore production process does not require high oxygen and requires relatively dry atmosphere conditions. Therefore, solid spore fermentation of biopesticides usually adopts shallow tray fermentation or layer fermentation. For example, CN201310040503.7 discloses a combined solid fermentation device, which is composed of multiple solid fermentation drawers and bases stacked on each other; CN2013100 32264.0 discloses a thin layer automatic static solid fermentation tank integrated optimization method and device, which places the sterilized and inoculated solid fermentation material on a disposable thin layer of auxiliary materials and a multi-layer movable fermentation bed in the sterile fermentation room, and then automatically adjusts the sterile air volume, temperature, humidity, etc. according to different fermentation stages; CN201810972882.6 discloses a wind-controlled multifunctional solid fermentation tray device, which consists of an air humidification tank, a material sterilization drain and condensate receiving tank and a reflux device, which can adjust the air humidity to avoid ventilation causing material drying, and adopts a multi-tray solid fermentation method. These fermentation devices all adopt a disc or layered fermentation design, which is composed of multi-layer fermentation containers; the disadvantage is that the workload of loading and unloading fermentation materials for layered fermentation is large, the fermentation materials for disc fermentation or layered fermentation cannot be well mixed, the fermentation difference between the upper and lower layers of fermentation materials is large, and the fermentation material loading capacity in the fermentation equipment is small, which is not suitable for large-scale fermentation.

[0004] Considering the fermentation capacity of the fermentation equipment and the mixing of the fermented materials, some solid fermentation equipment draws on the structural design of liquid fermentation equipment and uses built-in stirring paddles to mix the materials; for example, CN91105478.2 discloses a solid fermentation process of increasing bacteria and its special equipment, and the solid fermentation tank body is a sealed double-layer structure, consisting of a tank body, a tank cover and a horizontal spiral agitator; CN201320422615.4 discloses a solid fermentation tank, whose tank body has a horizontal stirring paddle, and the discharge port is located at the lower end of the solid fermentation tank; CN202320202019.9 discloses a vertical solid fermentation device, by arranging a multi-layer stirring and partitioning group inside the tank body, the rotating stirring paddle fully stirs the solid material on the partition during solid fermentation. The common point of these fermentation equipment is that the stirring paddle inside the fermentation tank is used to stir the materials in the fermentation tank, and the uniform stirring helps the tank body to have uniform oxygen supply and heat transfer; the common disadvantage of these fermentation equipment is that in actual production, the stirring paddle cannot achieve truly uniform mass transfer and heat transfer. Solid materials are different from liquid materials, and it is easy to produce stirring dead corners, and a larger stirring force is required. The shear force generated by stirring will destroy the solid mycelium, resulting in a decrease in spore production; and due to the large resistance of stirring, it will consume a lot of power and generate heat; because solids are not like liquids, heat, gas and mass transfer are prone to unevenness, resulting in low final fermentation yield and uneven fermentation.

[0005] In summary, layer fermentation or shallow plate fermentation does not stir the solid fermentation bacteria and materials themselves, does not damage the mycelium, and is conducive to fungal spore production, but the fermentation charge volume is small; while in the stirred solid fermentation device, stirring makes it difficult to transfer air and heat evenly, destroys the mycelium, and is not conducive to fungal spore production. Utility Model Content

[0006] In view of the above problems, the purpose of the utility model is to provide a rotating cavity solid fermentation device with controllable atmosphere, which has the advantages of simple structure, easy operation and low cost, and is suitable for spore-forming solid fermentation of fungal biological pesticides.

[0007] The technical solution adopted by the utility model is: a rotary cavity solid fermentation device with controllable gas regulation, comprising a motor, a gas generating device and a double cone cavity that can rotate on the output shaft of the motor;

[0008] The interior of the double-cone cavity is a cavity, and the upper and lower ends of the double-cone cavity are respectively provided with openings, and the openings are provided with lids, and a three-way pipe is provided on the lids; a plurality of sensing probes, a gas inlet and a spray inlet are provided on the inner wall of the double-cone cavity; and the spray inlet is connected to an external spray device;

[0009] The gas generating device comprises a panel provided with a plurality of gas outlets, a gas filtering device, a gas mixing temperature control device, an air compressor, a pure oxygen oxygen generator and a central controller; one end of the gas outlet is connected to the gas inlet, and a gas filter is arranged between the gas outlet and the gas inlet; the other end of the gas outlet is connected to the gas filtering device and the gas mixing temperature control device in sequence, and the gas mixing temperature control device is respectively connected to the air compressor, the pure oxygen oxygen generator and the central controller; the central controller is also respectively connected to the sensing probe and the spray device.

[0010] Preferably, the rotating cavity solid fermentation device further comprises a fixed pillar; both ends of the double cone cavity are respectively connected to the output shaft of the motor, the motor is fixed on the fixed pillar, and the fixed pillar stands on the ground.

[0011] Preferably, a pressure gauge is also provided on the inner wall of the double-cone cavity.

[0012] Preferably, a plurality of protrusions are further provided on the inner wall of the double-cone cavity.

[0013] Preferably, the protrusion is a hemispherical protrusion.

[0014] Preferably, the spray device is connected to a sterile water storage tank.

[0015] Preferably, the gas filter is a membrane gas filtration device.

[0016] Preferably, the gas mixing temperature control device is a cavity structure, in which a gas heating device and a gas cooling device are arranged.

[0017] Preferably, the sensing probe is provided with a Bluetooth device, and the data detected by the sensing probe is transmitted to the central controller via the Bluetooth device.

[0018] Preferably, a plurality of endoscopes containing cameras are also installed on the inner wall of the double-cone cavity.

[0019] Beneficial effects of the above technical solution:

[0020] (1) The utility model comprehensively considers the characteristics of fungal solid spore fermentation and discloses a controllable gas-controlled rotating chamber solid fermentation device for biopesticide fermentation. The device is particularly suitable for the spore solid fermentation of fungal biopesticides.

[0021] (2) The controllable gas-controlled rotating cavity solid fermentation device disclosed in the utility model has the advantages of simple structure, easy operation and low cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1A schematic diagram of the main structure of a rotating cavity solid fermentation device with controllable gas regulation provided by one embodiment of the utility model;

[0023] Figure 2 A schematic diagram of the structure of a gas generating device provided in one embodiment of the utility model

[0024] Among them, 1-double cone cavity; 2-motor; 3-fixed support; 4-ground; 5-lid; 6-three-way pipe; 7-valve; 8-sensing probe; 9-gas inlet; 10-spray inlet; 11-gas filter; 12-protrusion; 13-pressure gauge; 14-gas outlet; 15-gas filtering device; 16-pipeline; 17-gas mixing temperature control device; 18-pure oxygen generator; 19-air compressor; 20-central controller. DETAILED DESCRIPTION

[0025] The present invention is further described below through specific embodiments. It should be pointed out that, for ordinary technicians in this field, several modifications and improvements can be made without departing from the principle of the present invention, and these should also be regarded as belonging to the protection scope of the present invention.

[0026] The contents not described in detail in the utility model specification belong to the common knowledge of the professional and technical personnel in this field.

[0027] Example 1

[0028] like Figure 1 and Figure 2 As shown, the utility model discloses a controllable gas-adjustable rotating cavity solid fermentation device, comprising a double-cone cavity 1 rotatable on a motor, a motor 2, a fixed support 3 and a gas generating device; both ends of the double-cone cavity 1 are respectively connected to the output shaft of the motor 2, the motor 2 is fixed on the fixed support 3, and the fixed support 3 stands on the ground 4;

[0029] Among them, Figure 1 As shown, the interior of the double-cone cavity 1 is a cavity, and the upper and lower ends of the double-cone cavity 1 are respectively provided with openings, which are covered by a cover 5, and a three-way pipe 6 is provided on the cover 5, and the opening and closing of the first pipe 6 are controlled by a valve 7; a pressure gauge 13 and a plurality of protrusions 12, a sensor probe 8, a gas inlet 9 and a spray inlet 10 are provided on the inner wall of the double-cone cavity 1; the spray inlet 10 is connected to an external spray device through a pipe;

[0030] like Figure 2As shown, the gas generating device includes a panel with multiple gas outlets 14, a gas filtering device 15, a gas mixing temperature control device 17, an air compressor 19, a pure oxygen generator 18 and a central controller 20; one end of the gas outlet 14 is connected to the gas inlet 9 on the double-cone cavity 1 through a pipeline, and a gas filter 11 is arranged between the gas outlet 14 and the gas inlet 9; the other end of the gas outlet 14 is connected to the gas filtering device 15, and the gas filtering device 15 is connected to the gas mixing temperature control device 17 through a pipeline 16, and the gas mixing temperature control device 17 is respectively connected to the air compressor 19, the pure oxygen generator 18, and the central controller 20; the central controller 20 is also respectively connected to the sensing probe 8 and the spray device.

[0031] During fermentation, the interior of the double-cone cavity 1 is used to load solid fermentation materials, and the maximum loading amount of solid fermentation materials is usually half of the total volume of the cavity; the fermentation materials can be loaded into the interior of the double-cone cavity 1 by opening the lid 5; a plurality of gas inlets 9 are provided on the inner wall of the double-cone cavity 1, and the gas inlet 9 is used to pass the gas required for fermentation into the interior of the double-cone cavity 1; a gas filter 11 is provided on the outside of the gas inlet 9, and the gas filter 11 is, for example, a membrane gas filter device, and the gas filter 11 is used to filter microorganisms that may exist in the air, thereby producing sterile air; during the fermentation process, the gas generated by the fermentation is discharged from the three-way pipe 6, and when the fermentation device is sterilized, high-pressure steam enters the interior of the double-cone cavity from the three-way pipe 6.

[0032] In order to improve the mixing of solid fermentation materials during the rotation of the cavity 1, a protrusion 12 is provided on the inner wall of the cavity. The protrusion 12 is preferably a hemispherical protrusion. The hemispherical protrusion can better increase the collision and mixing of the material with the inner wall of the cavity, so that during the fermentation process, the mixing of the solid material is more uniform by rotating the double-cone cavity 1; the sensing probe 8 is installed at multiple locations on the inner wall of the cavity, and the sensing probe 8 is used to measure the temperature, humidity, oxygen partial pressure and carbon dioxide partial pressure of each area in the double-cone cavity. The sensing probe 8 is provided with a Bluetooth device, and the data collected by the sensing probe is transmitted to the central controller 20 through the Bluetooth device.

[0033] Furthermore, in one embodiment, a plurality of endoscopes containing cameras are installed on the inner wall of the double-cone cavity 1, and the endoscopes are used to observe the interior of the double-cone cavity 1. The camera is provided with a Bluetooth device, and the data collected by the camera is transmitted to the central controller 20 via the Bluetooth device.

[0034] A plurality of spray inlets 10 (i.e., water inlets) are arranged on the inner wall of the double-cone cavity 1, and the spray inlet 10 is connected to an external spray device through a pipeline, and the spray device is connected to a central controller 20. The central controller 20 controls the spray device to spray sterile water into the fermentation material through the spray inlet 10 according to the required amount for humidification; the end of the spray device can be connected to a sterile water storage tank through a pipeline.

[0035] The gas filter device 15 is a conventional gas filter device, which is mainly used to remove possible dust and particulate matter in the gas; the gas mixing temperature control device 17 is a cavity structure, which has a gas heating device and a gas cooling device inside. The heating device can be an electric heating wire, and the gas cooling device can be an air compressor. The temperature and flow of the gas can be controlled according to the central controller 20: the central controller 20 receives the data transmitted by the sensing probe 8 (the temperature, humidity, oxygen partial pressure and carbon dioxide partial pressure of each area in the double cone cavity), and obtains the temperature, oxygen concentration, carbon dioxide concentration and humidity in the double cone cavity 1 based on the data analysis, so as to control the gas mixing control device 17 to adjust the gas flow of the air compressor 19 and the gas flow of the pure oxygen generator 18, and produce mixed air of a given temperature with any oxygen content of 20%-95%, so as to realize fine control of the solid fermentation in the cavity.

[0036] The air compressor 19 generates high-pressure air, and the proportion of oxygen in the high-pressure air is 20% of the normal proportion of oxygen in the air; the pure oxygen generator 18 generates high-pressure oxygen, and the purity of its oxygen is greater than 95%; the gas generating device uses controllable oxygen gas, and during the bacterial fermentation process, high oxygen concentration fermentation (oxygen concentration 90%-95%) is adopted in the growth period, so that the bacteria grow rapidly, and the time is reduced by more than 60% compared with the conventional fermentation. After the bacterial growth stage is completed, the oxygen concentration is restored to the normal level (oxygen concentration 20%), which is conducive to the bacteria entering the spore production stage; the controllable gas generation and gas supply method can significantly shorten the fermentation time and improve the equipment utilization rate.

[0037] The output shaft of the motor 2 is connected to the two ends of the double-cone cavity 1, for example, by flanges, bolts, etc. The motor 2 drives the double-cone cavity 1 to rotate at a required rotation rate; during the rotation process, continuous rotation is not required, and a back-and-forth rotation method can be adopted, for example, rotating 180 degrees clockwise first, and then rotating 180 degrees counterclockwise.

[0038] Example 2

[0039] Solid fermentation of Trichoderma harzianum:

[0040] Trichoderma harzianum is a biological pesticide fungus that produces green conidia and thick-walled spores; the solid fermentation process of Trichoderma harzianum is divided into a mycelium growth stage and a spore production stage; there are differences between the two stages. In the mycelium growth stage, Trichoderma harzianum grows vigorously, requires a large amount of oxygen and high humidity conditions, and produces a large amount of heat. The most suitable growth temperature for mycelium is 28-30°C; the weight ratio of the fermentation solid culture medium formula is: 8% molasses, 2% corn pulp, 35% husk, 10% bran, and 45% water.

[0041] After the fermentation solid culture medium is stirred evenly, the lid 5 of the controllable gas-controlled rotating cavity solid fermentation device is opened, the fermentation solid culture medium is added into the double cone cavity 1, the lid 5 is closed, and high-pressure steam is introduced through the three-way pipe 6. The high-pressure sterilization is carried out at 121°C for 30 minutes. During the sterilization process, the total pressure in the double cone cavity 1 is monitored by the pressure gauge 13.

[0042] After the high-pressure sterilization is completed, the double-cone cavity 1 is naturally cooled until the pressure of the cavity 1 is reduced to 1 standard atmospheric pressure. The air compressor 19 is turned on, and the gas mixing temperature control device 17 is controlled by the central controller 20 to control the gas temperature to 15°C. Sterile air at 15°C is introduced from the gas inlet 9 to cool the fermentation solid culture medium in the tank. The valve 7 is opened, and the hot air is discharged from the three-way pipe 6 to take away the heat. During the cooling process, the motor 2 is rotated back and forth. The motor 2 rotates 180 degrees clockwise first, and then rotates 180 degrees counterclockwise. It rotates back and forth in this way to evenly cool the fermentation solid culture medium in the double-cone cavity. When the sensing probe 8 determines that the material temperature of the fermentation solid culture medium has dropped to 28°C, the liquid fermentation liquid of Trichoderma harzianum is added through the three-way pipe 6 (the liquid fermentation liquid of Trichoderma harzianum is obtained by conventional liquid fermentation technology and is used as the strain for solid fermentation). The motor 2 is started and rotated 180 degrees clockwise, then 180 degrees counterclockwise, and so on, so that the fermentation solid culture medium in the double cone cavity 1 and the liquid fermentation liquid of Trichoderma harzianum are evenly mixed.

[0043] At this time, the pure oxygen generator 18 is turned on, and the gas mixing temperature control device 17 is controlled by the central controller 20 to control the gas temperature to 26°C, and the oxygen concentration of the gas is greater than 90%; at this time, Trichoderma harzianum begins to ferment, and during the fermentation process, the motor 2 is still kept rotating 180 degrees clockwise, then 180 degrees counterclockwise, and rotates back and forth to mix and ferment the solid material. The sensing probe 8 senses the temperature, humidity, oxygen concentration and carbon dioxide partial pressure in the double cone cavity 1, and the central controller 20 controls and adjusts the fermentation conditions; wherein, the temperature in the double cone cavity 1 is not higher than 30°C, the humidity is not lower than 95%, and the oxygen concentration is greater than 60%; the central controller controls the spray device to spray sterile water through the spray inlet 10 to maintain the humidity in the fermentation cavity 1; Trichoderma harzianum generates heat during the fermentation process, and the gas mixing temperature control device 17 adjusts the gas temperature and gas flow to control the temperature in the double cone cavity 1; the gas mixing temperature control device 17 adjusts the gas oxygen concentration and gas flow to control the oxygen concentration in the double cone cavity 1. The mycelial growth stage of Trichoderma harzianum is completed in 3 days under high oxygen concentration; the gas produced by fermentation is discharged from the three-way pipe 6; at this time, the concentration of carbon dioxide produced by the respiration of the bacteria shows an obvious downward inflection point, which is a judgment mark for the completion of the mycelial growth stage.

[0044] When the Trichoderma harzianum enters the spore production stage, the air compressor 19 is turned on, the oxygen generator 18 is turned off, and the gas temperature is controlled to 28°C and the oxygen concentration of the gas is 20% by the gas mixing temperature control device 17 through the central controller 20; during the fermentation process, the motor 2 is still kept rotating 180 degrees clockwise, then 180 degrees counterclockwise, and so on; the central controller controls the spray device to stop spraying sterile water, so that the humidity in the fermentation chamber 1 naturally decreases; the spore production of Trichoderma harzianum is maintained for 4 days to produce a large number of Trichoderma harzianum spores; at this time, the fermentation is completed, the lid 5 is opened, and the fermented material is released.

[0045] Example 3

[0046] Solid fermentation of Paecilomyces lilacinus:

[0047] Paecilomyces lilacinus is a biopesticide fungus that produces lilac conidia. The solid fermentation process of Paecilomyces lilacinus is divided into a mycelial growth stage and a spore production stage. There are differences between the two stages. In the mycelial growth stage, Paecilomyces lilacinus grows vigorously, requires a large amount of oxygen and high humidity conditions, and produces a large amount of heat. The most suitable growth temperature of the mycelium is 28-30°C. The weight ratio of the fermentation solid culture medium formula is: 5% sucrose, 2% soy peptone, 38% rice husk, 10% corn flour, and 45% water.

[0048] After the fermentation solid culture medium is stirred evenly, the lid 5 of the controllable gas-controlled rotating cavity solid fermentation device is opened, the fermentation solid culture medium is added into the double cone cavity 1, the lid 5 is closed, and high-pressure steam is introduced through the three-way pipe 6, and the high-pressure sterilization is carried out at 121° C. for 30 minutes.

[0049] After the high-pressure sterilization is completed, the double-cone cavity 1 is naturally cooled down until the pressure of the cavity 1 is reduced to 1 standard atmospheric pressure. The air compressor 19 is turned on, and the gas mixing temperature control device is controlled by the central controller 20 to control the gas temperature to 15°C. Sterile air at 15°C is introduced from the gas inlet 9 to cool the fermentation solid culture medium in the tank. The valve 7 is opened, and the hot air is discharged from the three-way pipe 6 to take away the heat. During the cooling process, the motor 2 is rotated back and forth. The motor 2 rotates 180 degrees clockwise first, and then rotates 180 degrees counterclockwise. It rotates back and forth in this way to evenly cool the fermentation solid culture medium in the double-cone cavity. When the sensing probe 8 determines that the material temperature of the fermentation solid culture medium has dropped to 28°C, the liquid fermentation liquid of Paecilomyces lilacinus is added through the three-way pipe 6 (the liquid fermentation liquid of Paecilomyces lilacinus is obtained by fermentation by conventional liquid technical methods and is used as the strain for solid fermentation), and the motor 2 is started to rotate 180 degrees clockwise, then 180 degrees counterclockwise, and so on, so that the fermentation solid culture medium in the double cone cavity 1 and the liquid fermentation liquid of Paecilomyces lilacinus are evenly mixed.

[0050] At this time, the pure oxygen generator 18 is turned on, and the gas mixing temperature control device 17 is controlled by the central controller 20 to control the gas temperature to 25°C, and the oxygen concentration of the gas is greater than 90%; at this time, the Paecilomyces lilacinus begins to ferment, and during the fermentation process, the motor 2 is still kept rotating 180 degrees clockwise, then 180 degrees counterclockwise, and rotates back and forth to mix and ferment the solid material. The sensing probe 8 senses the temperature, humidity, oxygen concentration and carbon dioxide partial pressure in the double cone cavity 1, and the central controller 20 controls and adjusts the fermentation conditions; wherein, the temperature in the double cone cavity 1 is not higher than 28°C, the humidity is not lower than 95%, and the oxygen concentration is greater than 50%; the central controller controls the spray device to spray sterile water through the spray inlet 10 to maintain the humidity in the fermentation cavity 1; Paecilomyces lilacinus generates heat during the fermentation process, and the gas mixing temperature control device 17 is used to adjust the gas temperature and gas flow to control the temperature in the double cone cavity 1; the gas mixing temperature control device 17 is used to adjust the gas oxygen concentration and gas flow to control the oxygen concentration in the double cone cavity 1. The hyphae growth stage of Paecilomyces lilacinus is completed in 3 days under high oxygen concentration; the gas produced by fermentation is discharged from the three-way pipe 6; at this time, the concentration of carbon dioxide produced by the respiration of the bacteria shows an obvious downward inflection point, which is a judgment mark for the completion of the hyphae growth stage.

[0051] When Paecilomyces lilacinus enters the spore production stage, the air compressor 19 is turned on, the oxygen generator 18 is turned off, and the gas temperature is controlled to 28° C. and the oxygen concentration of the gas is 20% by the gas mixing temperature control device 17 through the central controller 20; during the fermentation process, the motor 2 is still kept rotating 180 degrees clockwise, then 180 degrees counterclockwise, and so on; the central controller controls the spray device to stop spraying sterile water, so that the humidity in the fermentation chamber 1 naturally decreases; the spore production of Paecilomyces lilacinus is maintained for 4 days, and a large number of Paecilomyces lilacinus spores are produced; at this time, the fermentation is completed, the lid 5 is opened, and the fermented material is released.

[0052] Example 4

[0053] Solid fermentation of Metarhizium anisopliae:

[0054] Metarhizium is a biological pesticide fungus that produces yellow-green conidia. The solid fermentation process of Metarhizium is divided into a mycelium growth stage and a spore production stage. There are differences between the two stages. In the mycelium growth stage, Metarhizium grows vigorously, requiring a large amount of oxygen and high humidity conditions, and produces a large amount of heat. The most suitable growth temperature for mycelium is 28-30°C. The weight ratio of the fermentation solid culture medium formula is: 2% sucrose, 5% soy peptone, 38% rice husk, 10% broken rice, and 45% water.

[0055] After the fermentation solid culture medium is stirred evenly, the lid 5 of the controllable gas-controlled rotating cavity solid fermentation device is opened, the fermentation solid culture medium is added into the double cone cavity 1, the lid 5 is closed, and high-pressure steam is introduced through the three-way pipe 6, and the high-pressure sterilization is carried out at 121° C. for 30 minutes.

[0056] After the high-pressure sterilization is completed, the double-cone cavity 1 is naturally cooled until the pressure of the cavity 1 is reduced to 1 standard atmospheric pressure. The air compressor 19 is turned on, and the gas mixing temperature control device is controlled by the central controller 20 to control the gas temperature to 15°C. Sterile air at 15°C is introduced from the gas inlet 9 to cool the fermentation solid culture medium in the tank. The valve 7 is opened, and the hot air is discharged from the three-way pipe 6 to take away the heat. During the cooling process, the output shaft of the motor 2 is rotated back and forth by first rotating 180 degrees clockwise and then 180 degrees counterclockwise, and rotates back and forth in this way to evenly cool the fermentation solid culture medium in the double-cone cavity. When the sensing probe 8 determines that the material temperature of the fermentation solid culture medium has dropped to 28°C, the liquid fermentation liquid of Metarhizium anisopliae is added through the three-way pipe 6 (the liquid fermentation liquid of Metarhizium anisopliae is obtained by fermentation by conventional liquid technical methods and is used as the strain for solid fermentation). The motor 2 is started and rotated 180 degrees clockwise, then 180 degrees counterclockwise, and so on, so that the fermentation solid culture medium in the double cone cavity 1 and the liquid fermentation liquid of Metarhizium anisopliae are evenly mixed.

[0057] At this time, turn on the pure oxygen generator 18, and control the gas mixing temperature control device 17 through the central controller 20 to control the gas temperature to 25°C, and the oxygen concentration of the gas is greater than 90%; at this time, the green muscardine begins to ferment, and during the fermentation process, the output shaft of the motor 2 is still kept rotating 180 degrees clockwise, then 180 degrees counterclockwise, and rotates back and forth to mix and ferment the solid material. The sensing probe 8 senses the temperature, humidity, oxygen concentration and carbon dioxide partial pressure in the double cone cavity 1, and the central controller 20 controls and adjusts the fermentation conditions; wherein, the temperature in the double cone cavity 1 is not higher than 28°C, the humidity is not lower than 95%, and the oxygen concentration is greater than 50%; the central controller controls the spray device to spray sterile water through the spray inlet 10 to maintain the humidity in the fermentation cavity 1; the green muscardine generates heat during the fermentation process, and the gas mixing temperature control device 17 adjusts the gas temperature and gas flow to control the temperature in the double cone cavity 1; the gas mixing temperature control device 17 adjusts the gas oxygen concentration and gas flow to control the oxygen concentration in the double cone cavity 1. The mycelial growth stage of Metarhizium muscardine is completed in 3 days under high oxygen concentration; the gas produced by fermentation is discharged through the three-way pipe 6; at this time, the concentration of carbon dioxide produced by the respiration of the bacteria shows an obvious downward inflection point, which is a judgment mark for the completion of the mycelial growth stage.

[0058] When the green muscardine enters the spore production stage, turn on the air compressor 19, turn off the oxygen generator 18, and control the gas mixing temperature control device 17 through the central controller 20 to control the gas temperature to 28°C and the oxygen concentration of the gas to 20%; during the fermentation process, the output shaft of the motor 2 is still kept rotating 180 degrees clockwise, then 180 degrees counterclockwise, and so on; the central controller controls the spray device to stop spraying sterile water, so that the humidity in the fermentation chamber 1 naturally decreases; the spore production of green muscardine is maintained for 4 days, and a large number of green muscardine spores are produced; at this time, the fermentation is completed, the lid 5 is opened, and the fermented material is released.

[0059] Example 5

[0060] Solid fermentation of Beauveria bassiana:

[0061] Beauveria bassiana is a biological pesticide fungus that produces grayish white conidia. The solid fermentation process of Beauveria bassiana is divided into a mycelium growth stage and a spore production stage. There are differences between the two stages. In the mycelium growth stage, Beauveria bassiana grows vigorously, requires a large amount of oxygen and high humidity conditions, and produces a large amount of heat. The most suitable growth temperature for mycelium is 28-30°C. The weight ratio of the fermentation solid culture medium formula is: 2% sucrose, 5% bean dregs, 38% rice husks, 10% bran, and 45% water.

[0062] After the fermentation solid culture medium is stirred evenly, the lid 5 of the controllable gas-controlled rotating cavity solid fermentation device is opened, the fermentation solid culture medium is added into the double cone cavity 1, the lid 5 is closed, and high-pressure steam is introduced through the three-way pipe 6, and the high-pressure sterilization is carried out at 121° C. for 30 minutes.

[0063] After the high-pressure sterilization is completed, the double-cone cavity 1 is naturally cooled until the pressure of the cavity 1 is reduced to 1 standard atmospheric pressure. The air compressor 19 is turned on, and the gas mixing temperature control device is controlled by the central controller 20 to control the gas temperature to 15°C. Sterile air at 15°C is introduced from the gas inlet 9 to cool the fermentation solid culture medium in the tank. The valve 7 is opened, and the hot air is discharged from the three-way pipe 6 to take away the heat. During the cooling process, the output shaft of the motor 2 is rotated back and forth by first rotating 180 degrees clockwise and then 180 degrees counterclockwise, and rotates back and forth in this way to evenly cool the fermentation solid culture medium in the double-cone cavity. When the sensing probe 8 determines that the material temperature of the fermentation solid culture medium has dropped to 28° C., the liquid fermentation liquid of Beauveria bassiana is added through the three-way pipe 6 (the liquid fermentation liquid of Beauveria bassiana is obtained by fermentation using a conventional liquid technical method and is used as a solid fermentation strain), and the motor 2 is started to rotate 180 degrees clockwise, then 180 degrees counterclockwise, and so on, so that the fermentation solid culture medium in the double cone cavity 1 and the liquid fermentation liquid of Beauveria bassiana are evenly mixed.

[0064] At this time, the pure oxygen generator 18 is turned on, and the gas mixing temperature control device 17 is controlled by the central controller 20 to control the gas temperature to 25°C, and the oxygen concentration of the gas is greater than 90%; at this time, Beauveria bassiana begins to ferment, and during the fermentation process, the output shaft of the motor 2 is still kept rotating 180 degrees clockwise, then 180 degrees counterclockwise, and rotates back and forth to mix and ferment the solid material. The sensing probe 8 senses the temperature, humidity, oxygen concentration and carbon dioxide partial pressure in the double cone cavity 1, and the central controller 20 controls and adjusts the fermentation conditions; wherein, the temperature in the double cone cavity 1 is not higher than 28°C, the humidity is not lower than 95%, and the oxygen concentration is greater than 50%; the central controller controls the spray device to spray sterile water through the spray inlet 10 to maintain the humidity in the fermentation cavity 1; Beauveria bassiana generates heat during the fermentation process, and the gas mixing temperature control device 17 adjusts the gas temperature and gas flow to control the temperature in the double cone cavity 1; the gas mixing temperature control device 17 adjusts the gas oxygen concentration and gas flow to control the oxygen concentration in the double cone cavity 1. The hyphae growth stage of Beauveria bassiana is completed in 3 days under high oxygen concentration; the gas produced by fermentation is discharged from the three-way pipe 6; at this time, the concentration of carbon dioxide produced by the respiration of the bacteria shows an obvious downward inflection point, which is a judgment mark for the completion of the hyphae growth stage.

[0065] When Beauveria bassiana enters the spore production stage, the air compressor 19 is turned on, the oxygen generator 18 is turned off, and the gas temperature is controlled to 28° C. and the oxygen concentration of the gas is 20% by the gas mixing temperature control device 17 controlled by the central controller 20; during the fermentation process, the output shaft of the motor 2 is still kept rotating 180 degrees clockwise, then 180 degrees counterclockwise, and so on; the central controller controls the spray device to stop spraying sterile water, so that the humidity in the fermentation chamber 1 naturally decreases; the spore production of Beauveria bassiana is maintained for 4 days to produce a large number of Beauveria bassiana spores; at this time, the fermentation is completed, the lid 5 is opened, and the fermented material is released.

[0066] Example 6

[0067] Solid fermentation of Bacillus thunbergii:

[0068] Bacillus suyujin is a biological pesticide fungus that produces spores. The solid fermentation process of Bacillus suyujin is divided into a bacterial growth stage and a spore production stage. There are differences between the two stages. In the bacterial growth stage, the bacteria grow vigorously, requiring a large amount of oxygen and high humidity conditions, and generating a large amount of heat. The most suitable growth temperature for the bacteria is 33-35°C. The weight ratio of the fermentation solid culture medium formula is: 2% sucrose, 10% bean dregs, 33% rice husks, 5% bran, and 50% water.

[0069] After the fermentation solid culture medium is stirred evenly, the lid 5 of the controllable gas-controlled rotating cavity solid fermentation device is opened, the fermentation solid culture medium is added into the double cone cavity 1, the lid 5 is closed, and high-pressure steam is introduced through the three-way pipe 6, and the high-pressure sterilization is carried out at 121° C. for 30 minutes.

[0070] After the high-pressure sterilization is completed, the double-cone cavity 1 is naturally cooled until the pressure of the cavity 1 is reduced to 1 standard atmospheric pressure. The air compressor 19 is turned on, and the gas mixing temperature control device is controlled by the central controller 20 to control the gas temperature to 15°C. Sterile air at 15°C is introduced from the gas inlet 9 to cool the fermentation solid culture medium in the tank. The valve 7 is opened, and the hot air is discharged from the three-way pipe 6 to take away the heat. During the cooling process, the output shaft of the motor 2 is rotated back and forth by first rotating 180 degrees clockwise and then 180 degrees counterclockwise, and rotates back and forth in this way to evenly cool the fermentation solid culture medium in the double-cone cavity. When the sensing probe 8 determines that the material temperature of the fermentation solid culture medium has dropped to 30°C, the liquid fermentation liquid of Bacillus thuringiensis is added through the three-way pipe 6 (the liquid fermentation liquid of Bacillus thuringiensis is obtained by fermentation by conventional liquid technical methods and is used as a strain for solid fermentation). The motor 2 is started and rotated 180 degrees clockwise, then 180 degrees counterclockwise, and so on, so that the fermentation solid culture medium in the double cone cavity 1 and the liquid fermentation liquid of Bacillus thuringiensis are evenly mixed.

[0071] At this time, the pure oxygen generator 18 is turned on, and the gas mixing temperature control device 17 is controlled by the central controller 20 to control the gas temperature to 28°C, and the oxygen concentration of the gas is greater than 90%; at this time, Bacillus thunbergii begins to ferment, and during the fermentation process, the output shaft of the motor 2 is still kept rotating 180 degrees clockwise, then 180 degrees counterclockwise, and rotates back and forth to mix and ferment the solid material. The sensing probe 8 senses the temperature, humidity, oxygen concentration and carbon dioxide partial pressure in the double cone cavity 1, and the central controller 20 controls and adjusts the fermentation conditions; wherein, the temperature in the double cone cavity 1 is not higher than 35°C, the humidity is not lower than 98%, and the oxygen concentration is greater than 30%; the central controller controls the spray device to spray sterile water through the spray inlet 10 to maintain the humidity in the fermentation cavity 1; Bacillus thunbergii generates heat during the fermentation process, and the gas temperature and gas flow are adjusted by the gas mixing temperature control device 17 to control the temperature in the double cone cavity 1; the gas oxygen concentration and gas flow are adjusted by the gas mixing temperature control device 17 to control the oxygen concentration in the double cone cavity 1. The growth stage of Bacillus suyujinensis is completed in 36 hours under high oxygen concentration; the gas produced by fermentation is discharged from the three-way pipe 6; at this time, the concentration of carbon dioxide produced by bacterial respiration shows an obvious downward inflection point, which is a judgment mark for the completion of the bacterial growth stage.

[0072] When Bacillus thunbergii enters the spore production stage, the air compressor 19 is turned on, the oxygen generator 18 is turned off, and the gas temperature is controlled to 30°C and the oxygen concentration of the gas is 20% by the gas mixing temperature control device 17 controlled by the central controller 20; during the fermentation process, the output shaft of the motor 2 is still kept rotating 180 degrees clockwise, then 180 degrees counterclockwise, and so on; the central controller controls the spray device to stop spraying sterile water, so that the humidity in the fermentation chamber 1 naturally decreases; the spore production of Bacillus thunbergii is maintained for 2 days, and a large number of Bacillus thunbergii spores are produced; at this time, the fermentation is completed, the lid 5 is opened, and the fermented material is released.

[0073] Example 7

[0074] Solid fermentation of Bacillus subtilis:

[0075] Bacillus subtilis is a biological pesticide fungus that produces spores. The solid fermentation process of Bacillus subtilis is divided into a bacterial growth stage and a spore production stage. There are differences between the two stages. In the bacterial growth stage, the bacteria grow vigorously, requiring a large amount of oxygen and high humidity conditions, and generating a large amount of heat. The most suitable growth temperature for the bacteria is 35-37°C. The formula weight ratio of the fermentation solid culture medium is: 2% sucrose, 10% bean dregs, 33% rice husks, 7% bran, and 48% water.

[0076] After the fermentation solid culture medium is stirred evenly, the lid 5 of the controllable gas-controlled rotating cavity solid fermentation device is opened, the fermentation solid culture medium is added into the double cone cavity 1, the lid 5 is closed, and high-pressure steam is introduced through the three-way pipe 6, and the high-pressure sterilization is carried out at 121° C. for 30 minutes.

[0077] After the high-pressure sterilization is completed, the double-cone cavity 1 is naturally cooled until the pressure of the cavity 1 is reduced to 1 standard atmospheric pressure. The air compressor 19 is turned on, and the gas mixing temperature control device is controlled by the central controller 20 to control the gas temperature to 15°C. Sterile air at 15°C is introduced from the gas inlet 9 to cool the fermentation solid culture medium in the tank. The valve 7 is opened, and the hot air is discharged from the three-way pipe 6 to take away the heat. During the cooling process, the output shaft of the motor 2 is rotated back and forth by first rotating 180 degrees clockwise and then 180 degrees counterclockwise, and rotates back and forth in this way to evenly cool the fermentation solid culture medium in the double-cone cavity. When the sensing probe 8 determines that the material temperature of the fermentation solid culture medium has dropped to 33°C, the liquid fermentation liquid of Bacillus subtilis is added through the three-way pipe 6 (the liquid fermentation liquid of Bacillus subtilis is obtained by fermentation by conventional liquid technical methods and is used as the strain for solid fermentation), and the motor 2 is started to rotate 180 degrees clockwise, then 180 degrees counterclockwise, and so on, so that the fermentation solid culture medium in the double cone cavity 1 and the liquid fermentation liquid of Bacillus subtilis are evenly mixed.

[0078] At this time, the pure oxygen generator 18 is turned on, and the gas mixing temperature control device 17 is controlled by the central controller 20 to control the gas temperature to 33°C, and the oxygen concentration of the gas is greater than 90%; at this time, Bacillus subtilis begins to ferment, and during the fermentation process, the output shaft of the motor 2 is still kept rotating 180 degrees clockwise, then 180 degrees counterclockwise, and rotates back and forth to mix and ferment the solid material. The sensing probe 8 senses the temperature, humidity, oxygen concentration and carbon dioxide partial pressure in the double cone cavity 1, and the central controller 20 controls and adjusts the fermentation conditions; wherein, the temperature in the double cone cavity 1 is not higher than 37°C, the humidity is not lower than 98%, and the oxygen concentration is greater than 30%; the central controller controls the spray device to spray sterile water through the spray inlet 10 to maintain the humidity in the fermentation cavity 1; Bacillus subtilis generates heat during the fermentation process, and the gas mixing temperature control device 17 is used to adjust the gas temperature and gas flow to control the temperature in the double cone cavity 1; the gas mixing temperature control device 17 is used to adjust the gas oxygen concentration and gas flow to control the oxygen concentration in the double cone cavity 1. The growth stage of Bacillus subtilis is completed in 36 hours under high oxygen concentration; the gas produced by fermentation is discharged from the three-way pipe 6; at this time, the concentration of carbon dioxide produced by bacterial respiration shows an obvious downward inflection point, which is a judgment mark for the completion of the bacterial growth stage.

[0079] When Bacillus subtilis enters the spore production stage, the air compressor 19 is turned on, the oxygen generator 18 is turned off, and the gas temperature is controlled to 32° C. and the oxygen concentration of the gas is 20% by the gas mixing temperature control device 17 controlled by the central controller 20; during the fermentation process, the output shaft of the motor 2 is still kept rotating 180 degrees clockwise, then 180 degrees counterclockwise, and so on; the central controller controls the spray device to stop spraying sterile water, so that the humidity in the fermentation chamber 1 naturally decreases; the spore production of Bacillus subtilis is maintained for 2 days to produce a large number of Bacillus subtilis spores; at this time, the fermentation is completed, the lid 5 is opened, and the fermented material is released.

[0080] Example 8

[0081] Solid fermentation of Bacillus amyloliquefaciens:

[0082] Bacillus amyloliquefaciens is a biological pesticide fungus that produces spores. The solid fermentation process of Bacillus amyloliquefaciens is divided into a bacterial growth stage and a spore production stage. There are differences between the two stages. In the bacterial growth stage, the bacteria grow vigorously, requiring a large amount of oxygen and high humidity conditions, and generating a large amount of heat. The most suitable growth temperature for the bacteria is 35-37°C. The weight ratio of the fermentation solid culture medium formula is: 1% sucrose, 10% bean dregs, 33% rice husks, 8% bran, and 48% water.

[0083] After the fermentation solid culture medium is stirred evenly, the lid 5 of the controllable gas-controlled rotating cavity solid fermentation device is opened, the fermentation solid culture medium is added into the double cone cavity 1, the lid 5 is closed, and high-pressure steam is introduced through the three-way pipe 6, and the high-pressure sterilization is carried out at 121° C. for 30 minutes.

[0084] After the high-pressure sterilization is completed, the double-cone cavity 1 is naturally cooled down until the pressure of the cavity 1 is reduced to 1 standard atmospheric pressure. The air compressor 19 is turned on, and the gas mixing temperature control device is controlled by the central controller 20 to control the gas temperature to 15°C. Sterile air at 15°C is introduced from the gas inlet 9. The valve 7 is opened, and the hot air is discharged from the three-way pipe 6 to take away the heat, so as to cool the fermentation solid culture medium in the tank. During the cooling process, a back-and-forth rotation method is adopted. The output shaft of the motor 2 rotates 180 degrees clockwise first, and then rotates 180 degrees counterclockwise, and rotates back and forth in this way, so that the fermentation solid culture medium in the double-cone cavity is evenly cooled. When the sensing probe 8 determines that the material temperature of the fermentation solid culture medium has dropped to 33°C, the liquid fermentation liquid of Bacillus amyloliquefaciens is added through the three-way pipe 6 (the liquid fermentation liquid of Bacillus amyloliquefaciens is obtained by fermentation by conventional liquid technical methods and is used as the strain for solid fermentation), and the motor 2 is started to rotate 180 degrees clockwise, then 180 degrees counterclockwise, and so on, so that the fermentation solid culture medium in the double cone cavity 1 and the liquid fermentation liquid of Bacillus amyloliquefaciens are evenly mixed.

[0085] At this time, the pure oxygen generator 18 is turned on, and the gas mixing temperature control device 17 is controlled by the central controller 20 to control the gas temperature to 33°C, and the oxygen concentration of the gas is greater than 90%; at this time, Bacillus amyloliquefaciens begins to ferment, and during the fermentation process, the output shaft of the motor 2 is still kept rotating 180 degrees clockwise, then 180 degrees counterclockwise, and rotates back and forth to mix and ferment the solid material. The sensing probe 8 senses the temperature, humidity, oxygen concentration and carbon dioxide partial pressure in the double cone cavity 1, and the central controller 20 controls and adjusts the fermentation conditions; wherein, the temperature in the double cone cavity 1 is not higher than 37°C, the humidity is not lower than 98%, and the oxygen concentration is greater than 40%; the central controller controls the spray device to spray sterile water through the spray inlet 10 to maintain the humidity in the fermentation cavity 1; Bacillus amyloliquefaciens generates heat during the fermentation process, and the gas temperature and gas flow are adjusted by the gas mixing temperature control device 17 to control the temperature in the double cone cavity 1; the gas oxygen concentration and gas flow are adjusted by the gas mixing temperature control device 17 to control the oxygen concentration in the double cone cavity 1. The growth stage of Bacillus amyloliquefaciens is completed in 36 hours under high oxygen concentration; the gas produced by fermentation is discharged through the three-way pipe 6; at this time, the carbon dioxide concentration shows an obvious downward inflection point, which is a judgment mark for the completion of the bacterial growth stage.

[0086] When Bacillus amyloliquefaciens enters the spore production stage, the air compressor 19 is turned on, the oxygen generator 18 is turned off, and the gas temperature is controlled to 32° C. and the oxygen concentration of the gas is 20% by the gas mixing temperature control device 17 controlled by the central controller 20; during the fermentation process, the output shaft of the motor 2 is still kept rotating 180 degrees clockwise, then 180 degrees counterclockwise, and so on; the central controller controls the spray device to stop spraying sterile water, so that the humidity in the fermentation chamber 1 naturally decreases; the spore production of Bacillus amyloliquefaciens is maintained for 2 days to produce a large number of spores of Bacillus amyloliquefaciens; at this time, the fermentation is completed, the lid 5 is opened, and the fermented material is released.

[0087] The present invention is described in detail above in combination with specific implementation methods and exemplary examples. The above description is exemplary, not exhaustive, and is not limited to the disclosed embodiments; the above description cannot be understood as a limitation of the present invention. Those skilled in the art understand that, without departing from the spirit and scope of the present invention, the technical solution of the present invention and its implementation methods can be subjected to a variety of equivalent substitutions, modifications or improvements, all of which fall within the scope of the present invention; the protection scope of the present invention shall be subject to the attached claims.

Claims

1. A rotating cavity solid fermentation device with controllable atmosphere, characterized in that: It comprises a motor (2), a gas generating device and a double cone cavity (1) rotatable on the output shaft of the motor; The interior of the double-cone cavity (1) is a cavity, and the upper and lower ends of the double-cone cavity (1) are respectively provided with openings, and a cover (5) is provided at the opening, and a three-way pipe (6) is provided on the cover (5); a plurality of sensing probes (8), a gas inlet (9) and a spray inlet (10) are provided on the inner wall of the double-cone cavity (1); the spray inlet (10) is connected to an external spray device; The gas generating device comprises a panel provided with a plurality of gas outlets (14), a gas filtering device (15), a gas mixing temperature control device (17), an air compressor (19), a pure oxygen generator (18) and a central controller (20); one end of the gas outlet (14) is connected to the gas inlet (9), and a gas filter (11) is provided between the gas outlet (14) and the gas inlet (9); the other end of the gas outlet (14) is connected to the gas filtering device (15) and the gas mixing temperature control device (17) in sequence, and the gas mixing temperature control device (17) is respectively connected to the air compressor (19), the pure oxygen generator (18) and the central controller (20); the central controller (20) is also respectively connected to the sensing probe (8) and the spray device.

2. The rotating cavity solid fermentation device according to claim 1, characterized in that: The rotating cavity solid fermentation device also includes a fixed support (3); the two ends of the double cone cavity (1) are respectively connected to the output shaft of the motor (2), the motor (2) is fixed on the fixed support (3), and the fixed support (3) stands on the ground (4).

3. The rotating cavity solid fermentation device according to claim 1, characterized in that: A pressure gauge (13) is also provided on the inner wall of the double-cone cavity (1).

4. The rotating cavity solid fermentation device according to claim 1, characterized in that: A plurality of protrusions (12) are also provided on the inner wall of the double-cone cavity (1).

5. The rotating cavity solid fermentation device according to claim 4, characterized in that: The protrusion (12) is a hemispherical protrusion.

6. The rotating cavity solid fermentation device according to claim 1, characterized in that: The spray device is connected to the sterile water storage tank.

7. The rotating cavity solid fermentation device according to claim 1, characterized in that: The gas filter (11) is a membrane gas filtering device.

8. The rotating cavity solid fermentation device according to claim 1, characterized in that: The gas mixing temperature control device (17) is a cavity structure, and a gas heating device and a gas cooling device are arranged inside the cavity.

9. The rotating cavity solid fermentation device according to claim 1, characterized in that: The sensing probe (8) is provided with a Bluetooth device, and the data detected by the sensing probe (8) is transmitted to the central controller (20) via the Bluetooth device.

10. The rotating cavity solid fermentation device according to claim 1, characterized in that: A plurality of endoscopes containing cameras are also installed on the inner wall of the double-cone cavity (1).

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