Flow type pipe with enrichment device

Abstract

The utility model discloses a flow type tube with a cell enrichment device. Relates to the technical field of cell enrichment and comprises a flow tube body, a top cover is arranged at the top of the flow tube body, an opening is formed in the bottom of the flow tube body, and a filter membrane is arranged at the opening and completely covers the opening. The top of the bottom cover is connected with the opening of the flow type pipe body, a through hole is formed in the side wall of the bottom cover, a protective cover is arranged at the through hole, and the protective cover is hinged to the side wall of the bottom cover; the negative pressure vacuum pump is arranged to generate negative pressure in the bottom cover, liquid in the flow type pipe body is affected by the negative pressure and is sucked into the bottom cover, cells are intercepted in the flow type pipe body through the filter membrane, and the cells are separated from the bottom cover through the negative pressure vacuum pump. And the problem of cell loss caused by a traditional centrifugal cell enrichment method is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of cell enrichment, and more specifically to a flow tube containing a cell enrichment device. Background Art

[0002] Flow tube is a cell container specially used in flow cytometry. It is used for specimen transportation, early cell enrichment, cell staining, washing, and later sample loading.

[0003] Current technology uses cerebrospinal fluid to enrich cells through centrifugation. After the cells are enriched and stained and incubated, they are washed with PBS through centrifugation. This method is likely to cause cell loss.

[0004] Therefore, how to provide a flow tube containing a cell enrichment device that can enrich cells under negative pressure without causing cell loss is an urgent problem that needs to be solved by those skilled in the art. Utility Model Content

[0005] In view of this, the utility model provides a flow tube containing a cell enrichment device, which enriches cells under negative pressure without causing cell loss.

[0006] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0007] A flow tube containing an enrichment device, comprising:

[0008] A flow tube body, wherein a top cover is arranged on the top of the flow tube body, an opening is arranged on the bottom of the flow tube body, a filter membrane is arranged at the opening, and the filter membrane completely covers the opening;

[0009] A bottom cover, the top of which is connected to the opening of the flow tube body, a through hole is arranged on the side wall of the bottom cover, a protective cover is arranged at the through hole, and the protective cover is hinged to the side wall of the bottom cover;

[0010] A negative pressure vacuum pump is connected to the interior of the bottom cover through the through hole.

[0011] Furthermore, the bottom cover is threadedly connected to the bottom of the flow tube body.

[0012] Furthermore, it also includes a pressure sensor, which is arranged on the negative pressure vacuum pump.

[0013] Furthermore, a connecting pipeline is arranged between the negative pressure vacuum pump and the bottom cover.

[0014] Furthermore, the pore size of the filter membrane is 5 μm.

[0015] It can be seen from the above technical solution that, compared with the prior art, the utility model discloses a flow tube containing a cell enrichment device, which generates negative pressure inside the bottom cover by setting a negative pressure vacuum pump. The liquid inside the flow tube body is affected by the negative pressure and sucked into the bottom cover. The cells are retained in the flow tube body by setting a filter membrane, thereby avoiding the problem of cell loss caused by the traditional centrifugal cell enrichment method. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings in the following description are only embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0017] Figure 1 A schematic diagram of the structure of a flow tube containing a cell enrichment device provided by the utility model;

[0018] Figure 2 A schematic diagram of the structure of the components of the flow tube containing the cell enrichment device provided by the utility model;

[0019] Figure 3 A schematic diagram of the structure of the flow tube and the bottom cover provided by the utility model;

[0020] Figure 4 for Figure 3 Schematic diagram of the cross section at AA;

[0021] Figure 5 This is a structural schematic diagram of the protective cover provided by the utility model.

[0022] Among them: 1 is the flow tube body; 2 is the top cover; 3 is the opening; 4 is the filter membrane; 5 is the bottom cover; 6 is the through hole; 7 is the negative pressure vacuum pump; 8 is the pressure sensor; 9 is the connecting pipeline; 10 is the protective cover. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] See also Figure 1-5 The utility model embodiment discloses a flow tube containing a cell enrichment device, comprising:

[0025] A flow tube body 1, a top cover 2 is arranged on the top of the flow tube body 1, an opening 3 is arranged on the bottom of the flow tube body 1, a filter membrane 4 is arranged at the opening 3, and the filter membrane 4 completely covers the opening 3; the body fluid and cells inside the flow tube body 1 are separated by arranging the filter membrane 4;

[0026] The bottom cover 5, the top of the bottom cover 5 is connected to the opening 3 of the flow tube body 1, the side wall of the bottom cover 5 is provided with a through hole 6, the through hole 6 is provided with a protective cover 10, and the protective cover 10 is hinged to the side wall of the bottom cover 5; the body fluid in the flow tube is affected by the negative pressure and is sucked into the bottom cover 5, and is stored and processed through the bottom cover 5. At the same time, the bottom cover 5 protects the filter membrane 4 to prevent the filter membrane 4 from being damaged, and facilitates the transportation of the flow tube body 1; the protective cover 10 is provided to avoid the problem of specimen loss during transportation;

[0027] The negative pressure vacuum pump 7 is connected to the interior of the bottom cover 5 through the through hole 6. By setting the negative pressure vacuum pump 7, negative pressure is generated inside the bottom cover 5, and the liquid inside the flow tube body 1 is affected by the negative pressure and is sucked into the bottom cover 5.

[0028] In this embodiment, the bottom cover 5 is threadedly connected to the bottom of the flow tube body 1 to facilitate the processing of body fluids.

[0029] In this embodiment, a pressure sensor 8 is also included. The pressure sensor 8 is arranged on the negative pressure vacuum pump 7. By arranging the pressure sensor 8 to monitor the pressure generated by the negative pressure vacuum pump 7, it is prevented that the generated negative pressure is too large to cause damage to the cells.

[0030] In this embodiment, a connecting pipe 9 is provided between the negative pressure vacuum pump 7 and the bottom cover 5 , and the negative pressure vacuum pump 7 is connected with the bottom cover 5 through the connecting pipe 9 , so that the negative pressure vacuum pump 7 can generate negative pressure in the bottom cover 5 .

[0031] In this embodiment, the pore size of the filter membrane 4 is 5 μm, and the filter membrane 4 can allow body fluid to flow into the bottom cover 5 while retaining cells in the flow tube body 1, thereby completing the enrichment of cells.

[0032] Specific usage

[0033] The flow tube body 1 is transported to the laboratory for enrichment;

[0034] Open the protective cover 10 provided at the through hole 6, connect the negative pressure vacuum pump 7 with the interior of the bottom cover 5 through the connecting pipe 9, turn on the negative pressure vacuum pump 7, so that the negative pressure vacuum pump 7 can generate negative pressure in the bottom cover 5, and monitor the pressure generated by the negative pressure vacuum pump 7 through the pressure sensor 8;

[0035] The liquid inside the flow tube body 1 is affected by the negative pressure and is sucked into the bottom cover 5. At the same time, the filter membrane 4 retains the cells inside the flow tube body 1. The bottom cover 5 is unscrewed from the bottom of the flow tube body 1, and the body fluid in the bottom cover 5 is poured out for processing to complete the cell enrichment.

[0036] In this specification, each embodiment is described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the embodiments can be referred to each other. For the device disclosed in the embodiment, since it corresponds to the method disclosed in the embodiment, the description is relatively simple, and the relevant parts can be referred to the method part.

[0037] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A flow tube containing an enrichment device, characterized in that: include: A flow tube body, wherein a top cover is arranged on the top of the flow tube body, an opening is arranged on the bottom of the flow tube body, a filter membrane is arranged at the opening, and the filter membrane completely covers the opening; A bottom cover, the top of which is connected to the opening of the flow tube body, a through hole is arranged on the side wall of the bottom cover, a protective cover is arranged at the through hole, and the protective cover is hinged to the side wall of the bottom cover; A negative pressure vacuum pump is connected to the interior of the bottom cover through the through hole.

2. A flow tube containing an enrichment device according to claim 1, characterized in that: The bottom cover is threadedly connected to the bottom of the flow tube body.

3. A flow tube containing an enrichment device according to claim 1, characterized in that: It also includes a pressure sensor, which is arranged on the negative pressure vacuum pump.

4. A flow tube containing an enrichment device according to claim 1, characterized in that: A connecting pipeline is arranged between the negative pressure vacuum pump and the bottom cover.

5. A flow tube containing an enrichment device according to claim 1, characterized in that: The pore size of the filter membrane is 5 μm.

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