Ultralow-temperature pseudovirus melting and centrifuging device

Abstract

The utility model relates to the technical field of experimental equipment, in particular to an ultralow-temperature pseudovirus melting centrifugal device which comprises a shell and a cover plate arranged on the shell, a centrifugal cavity is formed in the shell, and a coaxial rod in transmission connection with an output shaft of a rotor is arranged at the bottom in the centrifugal cavity; the bearing assembly comprises a centrifugal disc detachably arranged on the coaxial rod and a plurality of placing grooves which are formed in the centrifugal disc and used for placing centrifugal tubes; and the ultralow-temperature melting assembly is detachably arranged on the coaxial rod, is arranged on the lower end surface of the bearing assembly, and is used for melting the HPV neutralizing antibody pseudovirus liquid cryopreserved in the centrifugal tube while supporting the bearing assembly. According to the utility model, through the arrangement of the ultralow-temperature melting component, the ultralow-temperature pseudovirus melting function is realized, the trouble that other equipment is matched to melt an HPV neutralizing antibody pseudovirus solution at present is solved, and the convenience in operation is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of experimental equipment, in particular to an ultra-low temperature pseudovirus melting centrifuge. Background Art

[0002] Neutralizing antibodies are antigenic neutralizing sites that can specifically recognize the surface of the virus, prevent the virus from invading cells and multiplying, and protect the body from harm. They have a real antiviral effect. HPV neutralizing antibody pseudovirus contains neutralizing antigens of the virus and has the ability to infect cells; neutralizing antibodies can recognize HPV pseudoviruses and undergo a neutralizing reaction, reducing the ability of HPV pseudoviruses to infect, resulting in a decrease in the expression of the reporter genes (such as green fluorescent protein, luciferase, etc.) carried. The number of fluorescent spots in each well is scanned and counted by an Elispot reader, or the luciferase expression level in each well is read by a multifunctional microplate reader. The values ​​of the sample wells to be tested are compared with those of the pseudovirus control group, and the inhibition percentage of the sample to be tested can be calculated, thereby calculating the reciprocal of the dilution multiple of the antibody when 50% of the pseudovirus is inhibited, that is, the neutralizing antibody titer.

[0003] Normally, the HPV neutralizing antibody pseudovirus used for neutralizing antibody titer detection needs to be sealed and stored at -80℃±5℃. When diluting the pseudovirus, the pseudovirus needs to be taken out from the -80℃ ultra-low temperature refrigerator and placed on ice or at 4℃ to melt. After it is completely melted, it should be centrifuged briefly to allow the virus liquid to accumulate at the bottom of the storage tube. Then, a series of dilutions should be performed according to the pseudovirus titer reported and the experimental design (1TCID50=1 fluorescent spot).

[0004] However, the centrifugal device used in the prior art needs to wait until the HPV neutralizing antibody pseudovirus is melted in other external equipment before performing the centrifugal operation. This not only affects the experimental process and efficiency in the entire experimental operation, but also increases the frequency of use of other equipment, resulting in inevitable operational errors in the entire operation and overall troublesome operation.

[0005] The above contents are only used to assist in understanding the technical solution of the present invention, and do not constitute an admission that the above contents are the closest prior art. Utility Model Content

[0006] The utility model aims to solve the above-mentioned shortcomings and provide an ultra-low temperature pseudovirus melting centrifugal device.

[0007] In order to solve the above technical problems, the utility model adopts the following technical solutions: an ultra-low temperature pseudovirus melting centrifuge device comprises a shell, a cover plate arranged on the shell, a centrifugal chamber is arranged on the shell, and a coaxial rod connected to the output shaft of the rotor is arranged at the bottom of the centrifugal chamber;

[0008] The bearing assembly comprises a centrifugal disc detachably arranged on the coaxial rod, and a plurality of placement slots arranged on the centrifugal disc and used for placing centrifugal tubes;

[0009] The ultra-low temperature melting component is detachably arranged on the coaxial rod and arranged on the lower end surface of the bearing component, and is used for supporting the bearing component while melting the HPV neutralizing antibody pseudovirus liquid frozen and stored in the centrifuge tube.

[0010] Furthermore, the ultra-low temperature melting component includes a base detachably arranged on the coaxial rod and an upper loading plate arranged on the base, the base is provided with a storage cavity for storing ice cubes for melting the HPV neutralizing antibody pseudovirus liquid, the bottom of the storage cavity is connected to a water collecting box, the upper loading plate is provided with a plurality of bottom brackets having the same number as the placement slots, the center of the upper loading plate is provided with a support sleeve for being mounted and fixed on the outer wall of the coaxial rod, and the side supporting surface of the support sleeve is provided with a fan for generating airflow.

[0011] Furthermore, the bearing assembly also includes a groove arranged on the lower end surface of the centrifugal disk, an assembly set is arranged at the center of the top surface of the groove, the side support surface of the groove is provided with an array of air flow holes 1 with the same number as the placement groove, and the top surface of the groove is provided with several air flow holes 2 designed to pass through from top to bottom.

[0012] Furthermore, two symmetrically distributed positioning arc grooves are provided on the base.

[0013] Furthermore, the lower end surface of the upper carrier plate is provided with two symmetrically distributed positioning pins used in conjunction with corresponding positioning arc grooves.

[0014] Furthermore, an internal thread groove is provided at the top of the coaxial rod, and a fixing screw is provided in the internal thread groove for rotational cooperation.

[0015] Furthermore, the lower end opening of the base is communicated with the interior of the storage cavity.

[0016] Compared with the prior art, the utility model has the following beneficial effects: the utility model can not only support and fix the centrifuge tube placed on the supporting component, but also melt the HPV neutralizing antibody pseudovirus liquid taken out from the -80°C ultra-low temperature refrigerator, thereby realizing the ultra-low temperature pseudovirus melting function, solving the trouble of using other equipment to cooperate in the melting operation of the HPV neutralizing antibody pseudovirus liquid, and improving the convenience of operation; and through the provision of the air flow hole 1, the air flow hole 2 and the fan, the coordinated use of the above structure can drive the internal air flow, increase the contact area between the centrifuge tube and the external air flow, so that the HPV neutralizing antibody pseudovirus liquid stored in the centrifuge tube taken out from the -80°C ultra-low temperature refrigerator is quickly melted, further improving the experimental efficiency, and facilitating the centrifugation operation of the HPV neutralizing antibody pseudovirus liquid. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The drawings constituting part of the present application are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation on the present invention. In the drawings:

[0018] Figure 1 A three-dimensional structural diagram of an embodiment of the utility model from a single perspective;

[0019] Figure 2 It is a three-dimensional structural diagram of the overall front section of an embodiment of the utility model;

[0020] Figure 3 for Figure 2 The enlarged structural diagram at A in the middle;

[0021] Figure 4 It is a three-dimensional structural diagram of the front section of the load-bearing assembly and the ultra-low temperature melting assembly of one embodiment of the utility model;

[0022] Figure 5 for Figure 4 The structure diagram at B is enlarged;

[0023] Figure 6 It is a three-dimensional structural diagram of a bearing assembly according to an embodiment of the utility model;

[0024] Figure 7 It is a three-dimensional structural diagram of an ultra-low temperature melting assembly according to an embodiment of the present invention.

[0025] In the figure: 1. outer shell; 2. cover plate; 3. centrifugal chamber; 31. coaxial rod; 311. internal thread groove; 32. fixing screw; 4. bearing assembly; 41. centrifugal disc; 42. placement groove; 43. groove; 44. assembly set; 45. air flow hole 1; 46. air flow hole 2; 5. ultra-low temperature melting assembly; 51. base; 511. positioning arc groove; 52. upper loading plate; 521. positioning pin; 53. storage chamber; 54. water collecting box; 55. bottom bracket; 56. support sleeve; 561. fan. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. In the absence of conflict, the embodiments in this application and the features in the embodiments can be combined with each other. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] It should be noted that if the embodiments of the present invention involve directional indications (such as up, down, left, right, front, back...), the directional indications are only used to explain the relative position relationship, movement status, etc. between the components in a certain specific posture. If the specific posture changes, the directional indications will also change accordingly.

[0028] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the utility model, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the meaning of "and / or" appearing in the full text includes three parallel schemes. Taking "A and / or B" as an example, it includes scheme A, or scheme B, or schemes that satisfy both A and B. In addition, "multiple" refers to more than two. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in this field to implement. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the utility model.

[0029] like Figure 1-7 As shown, the ultra-low temperature pseudovirus melting centrifuge of the utility model comprises a shell 1, a cover plate 2 arranged on the shell 1, a centrifugal chamber 3 is arranged on the shell 1, and a coaxial rod 31 is arranged at the bottom of the centrifugal chamber 3 for transmission connection with the output shaft of the rotor;

[0030] The bearing assembly 4 comprises a centrifugal disc 41 detachably arranged on the coaxial rod 31, and a plurality of placement slots 42 arranged on the centrifugal disc 41 and used for placing centrifugal tubes;

[0031] The ultra-low temperature melting component 5 is detachably arranged on the coaxial rod 31 and arranged on the lower end surface of the bearing component 4, and is used to support the bearing component 4 while melting the HPV neutralizing antibody pseudovirus liquid frozen and stored in the centrifuge tube. In this design, by using the coaxial rod 31 connected by the rotor output shaft at the bottom of the centrifuge chamber 3, and sequentially sleeved and fixed the bearing component 4 and the ultra-low temperature melting component 5 from top to bottom on the outside of the coaxial rod 31, an ultra-low temperature melting function is added on the basis of the current centrifugal function, which can not only support and fix the centrifuge tube placed on the bearing component 4, but also melt the HPV neutralizing antibody pseudovirus liquid taken out from the -80°C ultra-low temperature refrigerator, which solves the trouble of using other equipment to cooperate in the melting operation of the HPV neutralizing antibody pseudovirus liquid and improves the convenience of operation.

[0032] It should be noted that the rotor designed inside the centrifugal chamber 3 and connected to the coaxial rod 31 is a horizontal rotor, which can make the virus liquid gather at the bottom of the storage tube under the centrifugal operation.

[0033] In one embodiment, the ultra-low temperature melting assembly 5 comprises a base 51 detachably arranged on the coaxial rod 31, an upper loading plate 52 arranged on the base 51, a storage chamber 53 for storing ice cubes for melting the HPV neutralizing antibody pseudovirus liquid is arranged on the base 51, and a water collecting box 54 is arranged at the bottom of the storage chamber 53. With this design, by sleeve-fixing the base 51 on the outside of the coaxial rod 31 and turning the storage chamber 53 for storing ice cubes on the base 51, ice cubes can be placed in the turned base 51 to perform ultra-low temperature melting operation on the HPV neutralizing antibody pseudovirus inside the centrifugal chamber 3, and the water collecting box 54 connected to the outer edge of the storage chamber 53 and turned on the base 51 can timely collect the liquid melted by the ice cubes to prevent liquid water from entering the centrifugal device and causing damage to the centrifugal device.

[0034] It should be noted that the interior of the water collection box 54 can be filled with a sponge with good water absorption.

[0035] It should be noted that a heat-insulating layer is pasted on the inner wall of the storage cavity 53 to ensure the integrity of the ice cubes, reduce the melting rate of the ice cubes, and ensure a good ultra-low temperature melting effect.

[0036] Preferably, the upper loading plate 52 is provided with a plurality of bottom brackets 55 having the same number as the placement slots 42, and a support sleeve 56 for being sleeved and fixed on the outer wall of the coaxial rod 31 is provided at the center of the upper loading plate 52, and a fan 561 for generating airflow is provided on the side support surface of the support sleeve 56. With such a design, the support sleeve 56 welded at the center of the upper surface of the upper loading plate 52 and the fan 561 fixed by screws in the groove machined on the side support surface of the support sleeve 56 can generate internal air flow, increase the contact area between the centrifuge tube and the external airflow, and ensure a good melting effect; and the bottom brackets 55 welded on the upper loading plate 52 corresponding to the placement slots 42 can provide good support for the bottom of the centrifuge tube placed in the placement slots 42 to prevent displacement.

[0037] In one embodiment, the bearing assembly 4 further comprises a groove 43 arranged on the lower end surface of the centrifugal disk 41, a mounting sleeve 44 is arranged at the center of the top surface of the groove 43, a side support surface of the groove 43 is provided with an array of air flow holes 45 having the same number as the placement groove 42, and a top surface of the groove 43 is provided with a plurality of air flow holes 46 designed to penetrate from top to bottom. With such a design, the bearing assembly 4 can be assembled outside the coaxial rod 31 by turning the groove 43 formed at the lower end of the centrifugal disk 41, and the mounting sleeve 44 welded in the center of the groove 43 and sleeved on the outside of the coaxial rod 31; the air flow holes 45 and the air flow holes 46 are also formed through the side support surface and the top surface of the groove 43 by turning, so as to form an air flow path inside and outside the centrifugal disk 41, thereby improving the ultra-low temperature pseudovirus melting effect.

[0038] In one embodiment, two symmetrically distributed positioning arc grooves 511 are provided on the base 51. In this design, the positioning arc groove 511 is machined at the center of the base 51, and the arc groove circumference of the positioning arc groove 511 opened on the base 51 is one quarter of a circle.

[0039] It should be noted that an upper portion of one end of the positioning arc groove 511 is provided with an open structure.

[0040] In one embodiment, the lower end surface of the upper loading plate 52 is provided with two symmetrically distributed positioning pins 521 used in conjunction with the corresponding positioning arc grooves 511. With this design, by welding two symmetrically distributed positioning pins 521 at the lower end of the upper loading plate 52, the two positioning pins 521 welded on the upper loading plate 52 can be butt-jointed and inserted into the positioning arc grooves 511 machined on the base 51 and then rotated, thereby achieving a fixed connection of the upper loading plate 52 on the base 51, and the overall operation is simple, providing convenience for subsequent placement of ice cubes.

[0041] It should be noted that the positioning pin 521 is L-shaped.

[0042] In one embodiment, the top of the coaxial rod 31 is provided with an internal thread groove 311, and a fixed screw 32 for rotational cooperation is provided in the internal thread groove 311. With this design, the bearing assembly 4 and the ultra-low temperature melting assembly 5 sequentially sleeved on the outside of the coaxial rod 31 can be limited and fixed by turning the internal thread groove 311 opened at the top of the coaxial rod 31, and rotating the fixed screw 32 on the internal thread groove 311, so as to complete the assembly of the bearing assembly 4 and the ultra-low temperature melting assembly 5 inside the centrifugal chamber 3, so as to realize the short centrifugation operation of the centrifuge tube storing the HPV neutralizing antibody pseudovirus liquid, so that the virus liquid is gathered at the bottom of the storage tube.

[0043] It should be noted that the height of the coaxial rod 31 is equal to the assembly height of the bearing assembly 4 and the ultra-low temperature melting assembly 5 , and the top limit plate of the fixed screw 32 is larger than the aperture of the assembly sleeve 44 .

[0044] In one embodiment, the lower end opening of the base 55 is connected to the interior of the storage chamber 53. With this design, through the opening through the bottom of the base 55, when ice cubes are stored in the storage chamber 53, the ice cubes can directly contact the bottom of the centrifuge tube through the opening at the bottom of the base 55, which can further achieve the effect of ultra-low temperature pseudovirus melting.

[0045] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention.

Claims

1. An ultra-low temperature pseudovirus melting centrifuge device, comprising a housing (1), a cover plate (2) arranged on the housing (1), wherein the housing (1) is provided with a centrifugal chamber (3), characterized in that: A coaxial rod (31) is provided at the bottom of the centrifugal chamber (3) and is transmission-connected to the output shaft of the rotor; The bearing assembly (4) comprises a centrifugal disc (41) detachably arranged on the coaxial rod (31), and a plurality of placement grooves (42) arranged on the centrifugal disc (41) and used for placing centrifugal tubes; The ultra-low temperature melting component (5) is detachably arranged on the coaxial rod (31) and arranged on the lower end surface of the bearing component (4), and is used to support the bearing component (4) while melting the HPV neutralizing antibody pseudovirus liquid frozen and stored in the centrifuge tube.

2. The ultra-low temperature pseudovirus melting centrifuge device according to claim 1, characterized in that: The ultra-low temperature melting assembly (5) comprises a base (51) detachably arranged on the coaxial rod (31) and an upper loading plate (52) arranged on the base (51); the base (51) is provided with a storage chamber (53) for storing ice cubes for melting HPV neutralizing antibody pseudovirus liquid; the bottom of the storage chamber (53) is connected to a water collecting box (54); the upper loading plate (52) is provided with a plurality of bottom brackets (55) having the same number as the placement grooves (42); the center of the upper loading plate (52) is provided with a support sleeve (56) for being sleeved and fixed on the outer wall of the coaxial rod (31); the side support surface of the support sleeve (56) is provided with a fan (561) for generating air flow.

3. The ultra-low temperature pseudovirus melting centrifugation device according to claim 1, characterized in that: The bearing assembly (4) further comprises a groove (43) arranged on the lower end surface of the centrifugal disc (41); a mounting sleeve (44) is arranged at the center of the top surface of the groove (43); a side support surface of the groove (43) is provided with an array of first air flow holes (45) having the same number as the placement groove (42); and a top surface of the groove (43) is provided with a plurality of second air flow holes (46) designed to penetrate from top to bottom.

4. The ultra-low temperature pseudovirus melting centrifuge device according to claim 2, characterized in that: The base (51) is provided with two symmetrically distributed positioning arc grooves (511).

5. The ultra-low temperature pseudovirus melting centrifugation device according to claim 4, characterized in that: The lower end surface of the upper carrier plate (52) is provided with two symmetrically distributed positioning pins (521) for use in conjunction with corresponding positioning arc grooves (511).

6. The ultra-low temperature pseudovirus melting centrifugation device according to claim 1, characterized in that: An internal thread groove (311) is provided at the top of the coaxial rod (31), and a fixing screw (32) is provided in the internal thread groove (311) for rotational cooperation.

7. The ultra-low temperature pseudovirus melting centrifugation device according to claim 2, characterized in that: The lower end opening of the base (55) is in communication with the interior of the storage cavity (53).

Images