Multi-channel DNA purification device

Abstract

The utility model belongs to the technical field of DNA purification by a paramagnetic particle method, and particularly relates to a multichannel DNA purification device. The multichannel DNA purification device comprises a chip, a heating device and a magnetic bead adsorption moving mechanism, a plurality of purification channels are arranged on the chip at intervals, and each purification channel comprises a first gas inlet, an inlet paraffin valve, a reaction cavity, an outlet paraffin valve and a waste liquid cavity which are communicated in sequence; the heating device can be used for heating the inlet paraffin valve, the reaction cavity, the outlet paraffin valve and the like in the reaction stage, the purging stage and the elution stage; and the magnetic bead adsorption moving mechanism can be used for adsorbing or driving the magnetic beads in the reaction cavity to move. The multi-channel DNA purification device disclosed by the utility model is high in integration level, can be completed on a chip, and is simple, convenient and efficient to operate; a plurality of purification channels are arranged, so that a plurality of samples can be operated on the same machine at the same or different time, and the efficiency is improved.

Description

Technical Field

[0001] The utility model belongs to the technical field of DNA purification by magnetic bead method, and specifically relates to a multi-channel DNA purification device. Background Art

[0002] The traditional laboratory magnetic bead method for DNA purification requires the use of magnetic beads with unique separation effects and a unique buffer system to separate and purify genomic DNA from samples such as animal tissues. The magnetic beads will adsorb and release nucleic acids under different conditions. The experimental steps first require the mixed reagents to be heated for reaction, that is, the experimenter adds buffer GHA and proteinase K to the PCR tube, shakes and mixes, and then places it in a water bath, heating at 65°C for 30 minutes. Shaking while heating helps the cleavage reaction; after the first heating is completed, GHB is added and shaken evenly, and heated at 75°C for 15 minutes; after the second heating is completed, isopropanol and magnetic bead suspension are added and shaken and left to stand for 10 minutes. After the reaction is completed, rinse with rinse solution for 3 minutes, blow dry with nitrogen for 5 minutes, and use a magnet to adsorb the magnetic beads on the outer wall of the PCR tube while rinsing and blowing dry; finally, add elution solution to elute the DNA on the magnetic beads.

[0003] However, in the process of purifying DNA by the traditional laboratory magnetic bead method, the PCR tube needs to be transferred between the processes of sample addition, water bath, shaking, elution, etc., and the operation is relatively complicated and has a high error rate. Utility Model Content

[0004] The utility model aims to provide a multi-channel DNA purification device to solve the technical problem of complex operation of the traditional laboratory magnetic bead method DNA purification process.

[0005] In order to solve the above technical problems, the utility model provides a multi-channel DNA purification device, comprising:

[0006] A chip; a plurality of purification channels are arranged at intervals on the chip, and the purification channels include a first air inlet, an inlet paraffin valve, a reaction chamber, an outlet paraffin valve and a waste liquid chamber that are connected in sequence;

[0007] A heating device, disposed on the lower side of the chip; and

[0008] The magnetic bead adsorption and movement mechanism is used to adsorb and drive the magnetic beads in the reaction chamber.

[0009] In one embodiment of the present application, the chip is further provided with a plurality of flushing channels;

[0010] The flushing channel comprises:

[0011] Second air inlet;

[0012] a liquid storage chamber, communicated with the second air inlet, and used for storing eluent; and

[0013] A plurality of flushing channels are provided, one end of which is communicated with the liquid storage cavity and the other end of which is communicated with the inlet paraffin valve, and the flushing channel is provided with a flushing paraffin valve.

[0014] In one embodiment of the present application, the flushing channel is disposed between two purification channels.

[0015] In one embodiment of the present application, the chip includes a top plate, a middle plate and a bottom plate arranged in sequence from top to bottom; wherein

[0016] The purification channel and the flushing channel are arranged on the bottom plate;

[0017] The top plate is provided with a sample adding port, a gas outlet and a liquid adding port which are respectively connected with the reaction chamber, the waste liquid chamber and the liquid storage chamber.

[0018] In one embodiment of the present application, the heating device includes heating plates respectively used to heat the reaction chamber, the inlet paraffin valve, the outlet paraffin valve and the flushing paraffin valve.

[0019] In one embodiment of the present application, the heating device includes a mounting plate disposed on the lower side of the chip;

[0020] A plurality of the heating plates are embedded on the mounting plate.

[0021] In one embodiment of the present application, the magnetic bead adsorption movement mechanism includes:

[0022] Magnetic members located on the upper and lower sides of the chip respectively; and

[0023] The sliding brackets are respectively used for installing corresponding magnetic parts.

[0024] The beneficial effects of the utility model are that the multi-channel DNA purification device of the utility model includes a chip, a heating device and a magnetic bead adsorption moving mechanism; a plurality of purification channels are arranged at intervals on the chip, and the purification channels include a first air inlet, an inlet paraffin valve, a reaction chamber, an outlet paraffin valve and a waste liquid chamber connected in sequence; the heating device can be used to heat the inlet paraffin valve, the reaction chamber, the outlet paraffin valve, etc. in the reaction stage, the purge stage, and the elution stage; the magnetic bead adsorption moving mechanism can be used to adsorb or drive the magnetic beads in the reaction chamber to move. The multi-channel DNA purification device of the utility model has a high degree of integration, can be completed on the chip, and is easy and efficient to operate; multiple purification channels are arranged, and multiple samples can be operated together on the same machine at the same time or at different times, which improves efficiency.

[0025] Other features and advantages of the utility model will be described in the following description, and partly become apparent from the description, or understood by practicing the utility model. The purpose and other advantages of the utility model are realized and obtained by the structures particularly pointed out in the description and the drawings.

[0026] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, preferred embodiments are given below and described in detail with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the specific implementation methods of the utility model or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0028] Figure 1 It is a schematic diagram of the assembly of the multi-channel DNA purification device of the utility model;

[0029] Figure 2 It is a schematic diagram of a chip base plate of a multi-channel DNA purification device of the utility model;

[0030] Figure 3 It is a schematic diagram of the chip coordination of the multi-channel DNA purification device of the utility model;

[0031] Figure 4 It is a schematic diagram of the assembly of the heating device of the multi-channel DNA purification device of the utility model.

[0032] In the figure:

[0033] Chip 1, top plate 11, sample addition port 123, air outlet 125, liquid addition port 152, middle plate 12, bottom plate 13, purification channel 2, first air inlet 21, inlet paraffin valve 22, reaction chamber 23, outlet paraffin valve 24, waste liquid chamber 25, heating device 3, heating plate 31, mounting plate 32, magnetic bead adsorption moving mechanism 4, magnetic part 41, sliding bracket 42, flushing channel 5, second air inlet 51, liquid storage chamber 52, flushing channel 53, flushing paraffin valve 54. DETAILED DESCRIPTION

[0034] In order to make the purpose, technical solution and advantages of the embodiments of the utility model clearer, the technical solution of the utility model will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0035] See also Figure 1 and Figure 2 In one embodiment of the present invention, the multi-channel DNA purification device comprises:

[0036] A chip 1; a plurality of purification channels 2 are arranged at intervals on the chip 1, and the purification channels 2 include a first air inlet 21, an inlet paraffin valve 22, a reaction chamber 23, an outlet paraffin valve 24 and a waste liquid chamber 25 which are connected in sequence;

[0037] A heating device 3 is disposed under the chip 1; and

[0038] The magnetic bead adsorption and movement mechanism 4 is used to adsorb and fix or drive the magnetic beads in the reaction chamber 23 to move.

[0039] In this embodiment, further, see Figure 2 The chip 1 is also provided with a plurality of flushing channels 5; the flushing channels 5 include: a second air inlet 51; a liquid storage chamber 52, which is connected to the second air inlet 51 and is used to store eluent; and a plurality of flushing channels 53, one end of which is connected to the liquid storage chamber 52 and the other end is connected to the inlet paraffin valve 22, and a flushing paraffin valve 54 is provided on the flushing channel 53.

[0040] In this embodiment, both the first air inlet 21 and the second air inlet 51 can be used for connecting to an external pump.

[0041] See also Figure 2 Optionally, the flushing channel 5 is arranged between the two purification channels 2; the liquid storage chamber 52 can be connected to the two reaction chambers 23 respectively through two flushing channels 53.

[0042] It should be noted that the paraffin valve can be melted by heating.

[0043] See also Figure 2 and Figure 3 As an optional embodiment of the chip 1, the chip 1 includes a top plate 11, a middle plate 12 and a bottom plate 13 arranged in sequence from top to bottom; wherein the purification channel 2 and the flushing channel 5 are arranged on the bottom plate 13; the top plate 11 is provided with a sample addition port 123, an air outlet 125 and a liquid addition port 152 which are respectively connected to the reaction chamber 23, the waste liquid chamber 25 and the liquid storage chamber 52.

[0044] See also Figure 1 and Figure 4 The heating device 3 includes a heating plate 31 for heating the reaction chamber 23, the inlet paraffin valve 22, the outlet paraffin valve 24 and the flushing paraffin valve 54 respectively, and can heat the corresponding heating objects according to the purification process.

[0045] Optionally, the heating device 3 includes a mounting plate 32 disposed on the lower side of the chip 1 ; a plurality of the heating plates 31 are embedded on the mounting plate 32 .

[0046] See also Figure 1 Furthermore, the magnetic bead adsorption moving mechanism 4 includes: magnetic members 41 respectively located on the upper side and the lower side of the chip 1; and sliding brackets 42 respectively used to install the corresponding magnetic members 41.

[0047] In this embodiment, optionally, the magnetic member 41 may be a permanent magnet or an electromagnet, which can be used to absorb magnetic beads; the sliding bracket 42 may be, but is not limited to, a linear motor, which can drive the magnetic member 41 to move back and forth, thereby driving the magnetic beads to oscillate.

[0048] In one application scenario, the purification process is:

[0049] During the preparation work, samples, reagents, etc. can also be added to the corresponding reaction chamber 23 from the upper sample addition port 11 as needed. Because the mixed reagent is highly hydrophilic, the reaction chamber 23 needs to be sealed with a paraffin valve, that is, the inlet paraffin valve 22 and the outlet paraffin valve 24 are used to seal to prevent overflow. Then, eluent is added to the liquid storage chamber 52 through the liquid addition port 152, and the flushing flow channel 53 is sealed by flushing the paraffin valve 54; the sample addition port 11 and the liquid addition port 152 are both sealed with a backing film.

[0050] The heating plate 31 corresponding to the bottom of the reaction chamber 23 starts to heat up, and during the heating process, the upper and lower magnetic parts 41 of the chip 1 are moved to control the vibration of the magnetic beads, which is helpful for cracking; when the reaction is finished, the heating plate 31 at the bottom of the inlet paraffin valve 22 and the outlet paraffin valve 24 starts to heat up to melt the paraffin, and the first air inlet 21 can pass the flushing liquid to flush the reaction chamber 23, and the flushed liquid enters the waste liquid chamber 25; after flushing, the first air inlet 21 can pass nitrogen to blow dry the reaction chamber 23; wherein, during the flushing and drying process, the magnetic part 41 at the bottom of the reaction chamber 23 can always keep the magnetic beads adsorbed and fixed to prevent the magnetic beads from being washed away; after drying, the heating plate 31 at the bottom of the flushing paraffin valve 54 is heated to melt the paraffin, and the second air inlet 51 can be connected to a pump to push the eluent of the liquid storage chamber 52 into the reaction chamber 23 to elute the DNA on the magnetic beads.

[0051] In summary, the multi-channel DNA purification device of the present invention can realize automated DNA purification of multiple samples on an integrated microfluidic chip, including heating reaction, shaking, flushing solution cleaning, nitrogen blowing and eluent dilution and other processes, with high integration and simple operation; in addition, compared with a single-channel purification device, the multi-channel purification device can perform purification operations on multiple samples at the same time or at different times, and the entire process is operated on the same machine, which reduces a large number of operating steps and time for professionals, avoids experimental errors caused by repeated operations, and saves a lot of manpower and material resources.

[0052] The various devices selected in this application (components whose specific structures are not described) are all universal standard parts or components known to technical personnel in this field, and their structures and principles can be known to technical personnel through technical manuals or through conventional experimental methods.

[0053] In the description of the embodiments of the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected" and "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection.

[0054] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0055] Based on the above ideal embodiments of the utility model, the relevant staff can make various changes and modifications without deviating from the technical concept of the utility model through the above description. The technical scope of the utility model is not limited to the content of the specification, and its technical scope must be determined according to the scope of the claims.

Claims

1. A multi-channel DNA purification device, characterized in that: include: A chip; a plurality of purification channels are arranged at intervals on the chip, and the purification channels include a first air inlet, an inlet paraffin valve, a reaction chamber, an outlet paraffin valve and a waste liquid chamber that are connected in sequence; A heating device, disposed on the lower side of the chip; and The magnetic bead adsorption and movement mechanism is used to adsorb and fix or drive the magnetic beads in the reaction chamber to move.

2. The multi-channel DNA purification device according to claim 1, characterized in that: The chip is also provided with a plurality of flushing channels; The flushing channel comprises: Second air inlet; a liquid storage chamber, communicated with the second air inlet, and used for storing eluent; and A plurality of flushing channels are provided, one end of which is communicated with the liquid storage cavity and the other end of which is communicated with the inlet paraffin valve, and the flushing channel is provided with a flushing paraffin valve.

3. The multi-channel DNA purification device according to claim 2, characterized in that: The flushing channel is arranged between two purification channels.

4. The multi-channel DNA purification device according to claim 2, characterized in that: The chip includes a top plate, a middle plate and a bottom plate arranged in sequence from top to bottom; wherein The purification channel and the flushing channel are arranged on the bottom plate; The top plate is provided with a sample adding port, a gas outlet and a liquid adding port which are respectively connected with the reaction chamber, the waste liquid chamber and the liquid storage chamber.

5. The multi-channel DNA purification device according to claim 2, characterized in that: The heating device comprises heating plates respectively used for heating the reaction chamber, the inlet paraffin valve, the outlet paraffin valve and the flushing paraffin valve.

6. The multi-channel DNA purification device according to claim 5, characterized in that: The heating device includes a mounting plate disposed on the lower side of the chip; A plurality of the heating plates are embedded on the mounting plate.

7. The multi-channel DNA purification device according to claim 1, characterized in that: The magnetic bead adsorption moving mechanism comprises: Magnetic members located on the upper and lower sides of the chip respectively; and The sliding brackets are respectively used for installing corresponding magnetic parts.

Images