Immunohistochemical wet box convenient to clean
By designing an immunohistochemical wet box for easy cleaning, the combination of box cover, raised strips, drawers, partition plates and effluent joints is used to clean the slides in the wet box, solving the problem of cumbersome cleaning process and easy exposure of the slides in the prior art, improving the experimental efficiency and ensuring the protection of the slides.
Patent Information
- Application Number
- CN202421282872.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-05
- Publication Date
- 2025-05-02
- Estimated Expiration
- 2034-06-05
AI Technical Summary
After the incubation is completed, the existing immunohistochemical wet box needs to take out the slides for cleaning, which increases the experimental time, reduces the work efficiency, and easily leads to the exposure of other slides.
An immunohistochemical wet box is designed for easy cleaning. By setting the box cover, raised strips, drawers, partitions and effluent joints, the slides can be carried out in the wet box during cleaning, reducing the cleaning steps, and fixing the sealing cover by magnets to prevent other slides from being exposed.
It reduces working time, improves experimental efficiency, and ensures that the slides will not be exposed during the cleaning process, meeting the efficient experimental needs.
Smart Images
Figure CN222825545U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of immunohistochemical humidified boxes, in particular to an immunohistochemical humidified box which is easy to clean. Background Art
[0002] Immunohistochemistry is the application of the basic principle of immunology - antigen-antibody reaction, that is, the principle of specific binding of antigen and antibody. Through chemical reaction, the color developer of the labeled antibody is used to determine the antigen in the tissue cells, and to conduct localization, qualitative and relatively quantitative research on it. It is called immunohistochemistry. It is a branch of histochemistry. All substances with antigenicity in tissue cells, such as peptides, hormones, neurotransmitters, cytokines, receptors, surface antigens, etc. can be displayed by immunohistochemistry. Therefore, it is currently widely used in basic and clinical scientific research. The wet box is an essential instrument for immunohistochemistry. Its function is to keep it moist. After BSA blocking, add the primary antibody, usually at 37°C for one hour, or at room temperature for 2 hours, or at 4°C overnight. Since the volume of the sample liquid hole on the slide is small, the added sample liquid can easily evaporate and dry up. Once the sample liquid dries up, the immune reaction of the antigen and antibody and the staining of the tissue cells will fail. Therefore, the slide needs to be placed in a sealed box with high humidity to ensure that the sample is moist. Another function of the wet box is to avoid light. Most of the secondary antibodies used in immunohistochemistry are fluorescently labeled antibodies, which are easily quenched when exposed to light. Therefore, it should be incubated with the antigen in a dark environment to ensure the best staining effect.
[0003] However, after the incubation of the existing immunohistochemistry wet box, the slides need to be taken out for cleaning and then put back into the wet box before adding another reagent, which increases the time required for the experiment and reduces work efficiency. In addition, the commonly used wet boxes generally have a box body and a lid. If the lid is opened, all the slides in the box will be exposed at the same time, which often causes other slides to be exposed when processing one slide. Utility Model Content
[0004] The technical problem to be solved by the utility model is to overcome the existing defects and provide an immunohistochemical humidification box which is easy to clean. By arranging the box cover, raised strips, drawers, partitions and liquid seepage seams, when cleaning the glass slide, one end of the glass slide is placed on the raised strip and the other end is tilted upward, and then the cleaning liquid is poured on the glass slide for cleaning. After cleaning, the glass slide is directly placed in the incubation chamber, and there is no need to take out the glass slide for cleaning, thereby reducing working time, improving experimental efficiency, and effectively solving the problems in the background technology.
[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: an immunohistochemical humidification box that is easy to clean, comprising a box body, a box cover is arranged on the upper side of the box body, an incubation chamber is arranged on the inner side of the box body, a seepage seam is arranged on the inner bottom side of the incubation chamber, a raised strip is arranged on one side of the incubation chamber, a drawer is arranged on the inner bottom side of the box body, a partition plate is arranged on the inner side of the drawer, a sealing cover is arranged on the upper side of the incubation chamber, a No. 2 magnet is arranged on the outer ring side of the sealing cover, a groove is opened on the box cover corresponding to the position of the incubation chamber, and a No. 1 magnet is arranged on the ring side of the groove.
[0006] Furthermore, a buckle handle is provided on the upper side of the sealing cover, and a handle is provided on the outer side of the drawer.
[0007] Furthermore, the handle is fixedly connected to the drawer, and the buckle is fixedly connected to the sealing cover.
[0008] Furthermore, the drawer is movably connected to the box body, and the sealing cover is fixedly connected to the second magnet.
[0009] Furthermore, the box cover is fixedly connected to the No. 1 magnet, and the partition plate is connected to the drawer slot.
[0010] Furthermore, sealing gaskets are provided on the upper edge of the box body and the lower side of the box cover.
[0011] Compared with the prior art, the beneficial effects of the utility model are:
[0012] 1. The utility model provides a box cover, a raised strip, a drawer, a partition plate and a liquid seepage seam, so that when cleaning a glass slide, one end of the glass slide is placed on the raised strip and the other end is tilted upward, and then the cleaning liquid is poured on the glass slide for cleaning. After cleaning, the glass slide is directly placed in the incubation chamber, and there is no need to take out the glass slide for cleaning, thereby reducing working time and improving experimental efficiency.
[0013] 2. The utility model is provided with an incubation chamber, a drawer, a partition plate, a sealing cover, a No. 1 magnet and a No. 2 magnet. The sealing cover can be stably fixed on the box cover by the No. 1 magnet and the No. 2 magnet. When cleaning a single glass slide, it is only necessary to open the sealing cover of the corresponding incubation chamber for cleaning, and other glass slides in the box body will not be exposed, thereby meeting the use requirements. BRIEF DESCRIPTION OF THE DRAWINGS
[0014] Figure 1 It is a structural schematic diagram of the utility model;
[0015] Figure 2 It is a schematic diagram of the top view of the box cover in the utility model;
[0016] Figure 3 For this utility model Figure 1A schematic diagram of the enlarged structure at A in the middle;
[0017] Figure 4 For this utility model Figure 1 Schematic diagram of the enlarged structure at point B in the middle.
[0018] In the figure: 1. box body; 2. box cover; 3. drawer; 4. handle; 5. partition; 6. incubation chamber; 7. raised strip; 8. sealing cover; 9. buckle; 10. seepage seam; 11. magnet No. 1; 12. magnet No. 2. DETAILED DESCRIPTION
[0019] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0020] See also Figure 1-4 The present embodiment provides a technical solution: an immunohistochemical humidified box that is easy to clean, comprising a box body 1, a box cover 2 is arranged on the upper side of the box body 1, an incubation chamber 6 is arranged on the inner side of the box body 1, a seepage seam 10 is arranged on the inner bottom side of the incubation chamber 6, a raised strip 7 is arranged on one side of the incubation chamber 6, a drawer 3 is arranged on the inner bottom side of the box body 1, a partition plate 5 is arranged on the inner side of the drawer 3, a sealing cover 8 is arranged on the upper side of the incubation chamber 6, a No. 2 magnet 12 is arranged on the outer ring side of the sealing cover 8, a groove is opened on the box cover 2 corresponding to the position of the incubation chamber 6, and a No. 1 magnet 11 is arranged on the ring side of the groove.
[0021] like Figure 1-4 As shown, magnet No. 1 11 and magnet No. 2 12 can firmly connect the sealing cover 8 and the box cover 2, prevent light from entering the incubation chamber 6 through the gap between the sealing cover 8 and the box cover 2, and prevent the glass slide from being exposed. The partition plate 5 can divide the inner side of the drawer 3 into six separate spaces, and then collect the liquid flowing out of the corresponding six incubation chambers 6.
[0022] A buckle handle 9 is provided on the upper side of the sealing cover 8 , and a handle 4 is provided on the outer side of the drawer 3 .
[0023] like Figure 1-4 As shown, the sealing cover 8 can be opened from the box cover 2 by the buckle 9, and the drawer 3 can be pulled out from the inner side of the box body 1 by the handle 4, so as to clean the liquid inside the drawer 3.
[0024] The handle 4 is fixedly connected to the drawer 3 , and the buckle handle 9 is fixedly connected to the sealing cover 8 .
[0025] The drawer 3 is movably connected to the box body 1 , and the sealing cover 8 is fixedly connected to the second magnet 12 .
[0026] The box cover 2 is fixedly connected to the No. 1 magnet 11, and the partition plate 5 is connected to the drawer 3 by a slot.
[0027] The upper edge of the box body 1 and the lower side of the box cover 2 are both provided with sealing pads.
[0028] The working principle of the immunohistochemical wet box provided by the utility model, which is easy to clean, is as follows: Figure 1-Figure 4 As shown, the sealing cover 8 is opened by the buckle 9, and then the glass slide is placed in the incubation chamber 6, and then the sealing cover 8 is covered on the box cover 2, so that the incubation chamber 6 remains dark. When the glass slide in a certain incubation chamber 6 needs to be cleaned, the sealing cover 8 of the corresponding incubation chamber 6 is opened, and then one end of the glass slide is pressed against the raised strip 7, and the other end is lifted upward, so that the end of the glass slide close to the raised strip 7 is in a low position, and then the cleaning liquid is poured on the glass slide, so that the cleaning liquid flows along the glass slide to the bottom side of the incubation chamber 6 and passes through the seepage seam 10. It flows into the space separated by the partition plate 5 in the drawer 3, ensuring the humidity inside the box body 1, and also ensuring the normal progress of the glass slide reaction. Through the incubation chamber 6, drawer 3, partition plate 5, sealing cover 8, No. 1 magnet 11 and No. 2 magnet 12, the sealing cover 8 can be stably fixed on the box cover 2 through the No. 1 magnet 11 and No. 2 magnet 12. When cleaning a single glass slide, it is only necessary to open the sealing cover 8 of the corresponding incubation chamber 6 for cleaning, and other glass slides in the box body 1 will not be exposed, thereby meeting the use requirements.
[0029] The above description is only an embodiment of the present invention, and does not limit the patent scope of the present invention. Any equivalent structure or equivalent process transformation made by using the contents of the specification and drawings of the present invention, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present invention.
Claims
1. An immunohistochemical wet box that is easy to clean, comprising a box body (1), characterized in that: A box cover (2) is arranged on the upper side of the box body (1), an incubation chamber (6) is arranged on the inner side of the box body (1), a liquid seepage seam (10) is arranged on the inner bottom side of the incubation chamber (6), a raised strip (7) is arranged on one inner side of the incubation chamber (6), a drawer (3) is arranged on the inner bottom side of the box body (1), a partition plate (5) is arranged on the inner side of the drawer (3), a sealing cover (8) is arranged on the upper side of the incubation chamber (6), a No. 2 magnet (12) is arranged on the outer ring side of the sealing cover (8), a groove is opened on the box cover (2) corresponding to the position of the incubation chamber (6), and a No. 1 magnet (11) is arranged on the ring side of the groove.
2. The immunohistochemical wet box that is easy to clean according to claim 1, characterized in that: The upper side of the sealing cover (8) is provided with a buckle (9), and the outer side of the drawer (3) is provided with a handle (4).
3. The immunohistochemical wet box that is easy to clean according to claim 2, characterized in that: The handle (4) is fixedly connected to the drawer (3), and the buckle (9) is fixedly connected to the sealing cover (8).
4. The immunohistochemical wet box that is easy to clean according to claim 1, characterized in that: The drawer (3) is movably connected to the box body (1), and the sealing cover (8) is fixedly connected to the second magnet (12).
5. The immunohistochemical wet box that is easy to clean according to claim 1, characterized in that: The box cover (2) is fixedly connected to the No. 1 magnet (11), and the partition plate (5) is connected to the drawer (3) through a slot.
6. The immunohistochemical wet box that is easy to clean according to claim 1, characterized in that: The upper edge of the box body (1) and the lower side of the box cover (2) are both provided with sealing gaskets.