Enzyme hydrolysis device

Abstract

The utility model relates to the technical field of enzyme reaction equipment, and provides an enzyme hydrolysis device aiming at the problems of non-uniform mixing and inconvenience in repeated utilization of enzyme in the conventional hydrolysis tank, which comprises a tank body, and the tank body is provided with a sealing cover, a feeding and discharging assembly, a mixing assembly and a heating assembly for enabling the inside of the tank to reach a proper reaction temperature, the sealing cover is movably connected with the top of the tank body; and an enzyme filling assembly comprising a mounting plate and a plurality of enzyme filling columns is arranged in the inner cavity of the tank body. After being conveyed to the inner cavity of the tank body through the feeding and discharging assembly, the waste grease raw material enters the enzyme filling column through the first through hole, the waste grease raw material and immobilized lipase are subjected to full contact reaction through the mixing assembly, and the hydrolysis effect is good; and the immobilized lipase is filled in the enzyme filling column, cannot be discharged along with a product and can be remained in the tank body to continuously participate in the reaction, and when the enzyme activity is reduced, the enzyme filling column can be taken out from the mounting plate by opening the sealing cover, so that the immobilized lipase is convenient to recycle, and the device is economical and practical.

Description

Technical Field

[0001] The utility model relates to the technical field of enzyme reaction equipment, in particular to an enzyme hydrolysis device. Background Art

[0002] my country's energy resources are relatively scarce, and with the rapid development of my country's economy, the demand for energy is increasing. The physical and chemical properties of biodiesel are very similar to those of petrochemical diesel, and it has attracted widespread attention as a renewable biomass energy source. The process of producing biodiesel using the bioenzymatic method has relatively mild reaction conditions, low requirements for oil raw materials, and can use waste oil raw materials. The reaction products are easy to separate, green and pollution-free, and have broad application prospects. Lipase is an enzyme that catalyzes fat hydrolysis or synthesis reactions. Usually, waste oil is first hydrolyzed into fatty acids and glycerol by lipase, and then the hydrolysis product is used as a raw material for esterification reaction to produce oil using lipase. The hydrolysis reaction requires a special hydrolysis device, and the existing hydrolysis devices generally have problems such as uneven mixing and low reaction efficiency.

[0003] The patent with publication number CN215887038U provides a waste oil enzymatic hydrolysis tank, including an enzymatic hydrolysis tank body for waste oil hydrolysis, and a circulation mechanism arranged on the enzymatic hydrolysis tank body for circulating hydrolysis; the circulation mechanism includes a circulating main liquid outlet connected from the bottom of the enzymatic hydrolysis tank body, and a first sub-circulation pipeline and a second sub-circulation pipeline respectively connected from the circulating main liquid outlet, the first sub-circulation pipeline is connected to the top of the enzymatic hydrolysis tank body, and the second sub-circulation pipeline is connected to the middle of the enzymatic hydrolysis tank body. Although the enzymatic hydrolysis tank enables the internal materials to be circulated and mixed in the horizontal and vertical directions, it enhances the full contact between the materials and the hydrolytic enzyme at various places, makes the hydrolysis more complete, and improves the hydrolysis efficiency and hydrolysis quality, however, it uses a hydrolytic enzyme liquid, that is, a liquid enzyme in the form of a solution, and there are still problems such as high cost of biological enzyme use, short life, difficulty in separation and recovery, and inconvenience in reuse.

[0004] Therefore, there is an urgent need to provide an enzymatic hydrolysis device that has good hydrolysis effect, recyclable enzymes, and is economical and practical. Utility Model Content

[0005] The purpose of the utility model is to solve the problems of uneven mixing and inconvenient enzyme reuse in existing hydrolysis tanks. The application provides an enzyme hydrolysis device, which can achieve good hydrolysis effect and the technical effect of enzyme recycling.

[0006] The utility model is realized through the following technical scheme: an enzyme hydrolysis device, comprising a tank body, wherein the tank body is provided with a sealing cover, a material inlet and outlet assembly, a material mixing assembly and a heating assembly, wherein the sealing cover is movably connected to the top of the tank body; an enzyme filling assembly is arranged in the inner cavity of the tank body, wherein the enzyme filling assembly comprises a mounting plate and a plurality of enzyme filling columns, wherein the mounting plate is mounted in the inner cavity of the tank body, the bottoms of the plurality of enzyme filling columns are detachably connected to the surface of the mounting plate, and the wall surface of the enzyme filling column is provided with a plurality of first through holes.

[0007] Preferably, the mixing assembly includes a speed regulating oscillator, an oscillation frame and multiple oscillation springs, one end of each of the oscillation springs is detachably connected to the top of the speed regulating oscillator, and the other end of each of the oscillation springs is detachably connected to the bottom of the oscillation frame, and the tank body is placed in the oscillation frame.

[0008] Preferably, the enzyme loading column is provided with a top cover, and the top of the top cover is provided with a pull ring.

[0009] Preferably, a plurality of mounting holes are provided on the surface of the mounting plate, internal threads are provided in the mounting holes, external threads are provided at the bottom of the enzyme loading column, and the internal threads cooperate with the external threads.

[0010] Preferably, a pair of limit rods are arranged in the oscillation frame, and the limit rods are arranged on both sides of the tank body. One end of the limit rod is detachably connected to the inner wall surface of the oscillation frame, and the other end of the limit rod is detachably connected to the outer wall surface of the tank body.

[0011] Preferably, the heating assembly comprises a jacket and an electric heating rod, the jacket is arranged on the outer wall surface of the tank body, and the electric heating rod is arranged in a containing space formed between the jacket and the tank body.

[0012] Preferably, the feed and discharge components include a raw material feed pipe and a product discharge pipe, the raw material feed pipe and the product discharge pipe are both connected to the top of the sealing cover, and the raw material feed pipe and the product discharge pipe are both equipped with a control valve and a liquid pump.

[0013] Preferably, the mounting plate is mounted at a position close to the bottom of the tank body, and the mounting plate is provided with a plurality of second through holes and long openings, and the product discharge pipe passes through the mounting plate through the long openings.

[0014] Preferably, an observation window is installed on the outer wall surface of the tank body.

[0015] The technical solution of the utility model has the following beneficial effects:

[0016] (1) When in use, the immobilized lipase is loaded into the inner cavity of the enzyme loading column, and then the enzyme loading column is installed in the mounting plate of the inner cavity of the tank; after the waste grease raw material is transported to the inner cavity of the tank through the material inlet and outlet assembly, the waste grease raw material enters the enzyme loading column through the first through hole, and the mixing assembly allows the waste grease raw material to fully contact and react with the immobilized lipase in the enzyme loading column. During the reaction process, the heating assembly is used to make the tank reach a suitable reaction temperature to promote the smooth progress of the hydrolysis reaction.

[0017] (2) The immobilized lipase loaded in the enzyme loading column will not be discharged with the product, but can remain in the tank to continuously participate in the reaction. When the enzyme activity decreases after multiple uses, the enzyme loading column can be removed from the mounting plate by opening the sealing cover, so that the immobilized lipase can be recycled through washing, regeneration and other steps, which is economical and practical.

[0018] (3) Turn on the speed regulating oscillator, adjust the oscillation frequency, and oscillate the oscillation frame and the tank placed inside the oscillation frame through the oscillation spring transmission. The oscillation mixing and the enzyme loading column following the oscillation can avoid the accumulation of glycerol with high viscosity and density on the immobilized lipase, and the enzyme has high stability, high reaction efficiency, and good hydrolysis effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions of the embodiments of the utility model, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the utility model and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without paying creative work.

[0020] Figure 1 It is a partial cross-sectional structural schematic diagram of the enzymatic hydrolysis device in Example 1;

[0021] Figure 2 This is a schematic diagram of the structure of the enzyme-packed column in Example 1;

[0022] Figure 3 It is a structural schematic diagram of the mounting plate in Example 1;

[0023] Figure 4 It is a structural schematic diagram of the tank body in Example 1 in a front view state.

[0024] Figure markings: 1-tank body, 11-observation window, 2-sealing cover, 21-pipe joint, 3-inlet and outlet assembly, 31-raw material feed pipe, 32-product discharge pipe, 4-mixing assembly, 41-speed regulating oscillator, 42-oscillation frame, 43-oscillation spring, 44-limiting rod, 5-heating assembly, 51-jacket, 52-electric heating rod, 6-enzyme loading assembly, 61-mounting plate, 611-second through hole, 612-mounting hole, 613-internal thread, 614-long strip opening, 62-enzyme loading column, 621-first through hole, 622-top cover, 623-pull ring, 624-external thread. DETAILED DESCRIPTION

[0025] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Generally, the components of the embodiments of the utility model described and shown in the drawings here can be arranged and designed in various different configurations.

[0026] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the present invention to be protected, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0028] Example 1

[0029] like Figures 1 to 4 As shown, this embodiment provides an enzymatic hydrolysis device, including a tank body 1, the tank body 1 is equipped with a sealing cover 2, a material inlet and outlet assembly 3, a material mixing assembly 4 and a heating assembly 5, and the sealing cover 2 is movably connected to the top of the tank body 1; the inner cavity of the tank body 1 is provided with an enzyme filling assembly 6, and the enzyme filling assembly 6 includes a mounting plate 61 and a plurality of enzyme filling columns 62, the mounting plate 61 is mounted in the inner cavity of the tank body 1, and the bottoms of the plurality of enzyme filling columns 62 are all screwed to the surface of the mounting plate 61, and the wall surface of the enzyme filling column 62 is provided with a plurality of first through holes 621, and the mounting plate 61 is provided with a plurality of second through holes 611. The mounting plate 61 and the enzyme filling column 62 are both made of stainless steel anti-corrosion materials.

[0030] When in use, the immobilized lipase is loaded into the inner cavity of the enzyme loading column 62, and then the enzyme loading column 62 is installed in the mounting plate 61 of the inner cavity of the tank body 1; after the waste grease raw material is transported to the inner cavity of the tank body 1 through the material inlet and outlet assembly 3, the waste grease raw material enters the enzyme loading column 62 through the first through hole 621, and the waste grease raw material is fully contacted and reacted with the immobilized lipase in the enzyme loading column 62 through the mixing assembly 4. During the reaction process, the heating assembly 5 is used to make the tank reach a suitable reaction temperature to promote the smooth progress of the hydrolysis reaction.

[0031] Moreover, the immobilized lipase loaded in the enzyme loading column 62 will not be discharged with the product, but can remain in the tank 1 to continuously participate in the reaction. When the enzyme activity decreases after multiple uses, the enzyme loading column 62 can be taken out from the mounting plate 61 by opening the sealing cover 2, so that the immobilized lipase can be recycled through washing, regeneration and other steps, which is economical and practical.

[0032] In this embodiment, the mixing component 4 includes a speed regulating oscillator 41, an oscillation frame 42 and a plurality of oscillation springs 43, one end of each of the plurality of oscillation springs 43 is screwed to the top of the speed regulating oscillator 41, and the other end of each of the plurality of oscillation springs 43 is screwed to the bottom of the oscillation frame 42, and the tank body 1 is placed in the oscillation frame 42. The speed regulating oscillator 41 is turned on, the oscillation frequency is adjusted, and the oscillation frame 42 and the tank body 1 placed in the oscillation frame 42 are oscillated through the transmission of the oscillation spring 43. The oscillation mixing is adopted, and the enzyme loading column 62 follows the oscillation to avoid the accumulation of glycerol with high viscosity and density on the immobilized lipase, and the enzyme has high stability, high reaction efficiency and good hydrolysis effect.

[0033] In this embodiment, the top cover 622 configured on the enzyme loading column 62 can facilitate loading or taking out the immobilized lipase. The pull ring 623 configured on the top of the top cover 622 can facilitate taking out the enzyme loading column 62.

[0034] In this embodiment, internal threads 613 are provided in the plurality of mounting holes 612 opened on the surface of the mounting plate 61, and external threads 624 are provided at the bottom of the enzyme loading column 62. The internal threads 613 and the external threads 624 cooperate with each other to facilitate the installation of the enzyme loading column 62 into the mounting plate 61, and also to facilitate the removal of the enzyme loading column 62.

[0035] In this embodiment, a pair of limiting rods 44 are disposed in the oscillation frame 42 and are disposed on both sides of the tank body 1. One end of the limiting rod 44 is screwed to the inner wall surface of the oscillation frame 42, and the other end of the limiting rod 44 is in contact with the outer wall surface of the tank body 1. An elastic rubber sleeve (not shown) is sleeved on one end of the limiting rod 44 close to the tank body 1, which is convenient for placing the tank body 1 and can increase friction. The limiting rod 44 can stably place the tank body 1 in the oscillation frame 42.

[0036] In this embodiment, the heating assembly 5 includes a jacket 51 and an electric heating rod 52. The jacket 51 is arranged on the outer wall of the tank body 1, and the electric heating rod 52 is arranged in the accommodation space formed between the jacket 51 and the tank body 1. The electrode of the electric heating rod 52 is arranged outside the jacket 51, and the temperature inside the tank is increased by the electric heating rod 52, which is conducive to promoting the hydrolysis reaction.

[0037] In this embodiment, the feed and discharge assembly 3 includes a raw material feed pipe 31 and a product discharge pipe 32. The raw material feed pipe 31 and the product discharge pipe 32 are both connected to the top of the sealing cover 2. The raw material feed pipe 31 and the product discharge pipe 32 are both equipped with a control valve (not shown) and a liquid pump (not shown). Raw materials can be introduced or products can be discharged in time according to the reaction situation to improve the reaction efficiency.

[0038] In this embodiment, the mounting plate 61 is set up near the bottom of the tank body 1, and the mounting plate 61 is provided with a plurality of second through holes 611 and a long strip opening 614. The glycerol generated by hydrolysis has a large density and high viscosity, and is easily deposited at the bottom of the tank body 1. Under natural conditions, it is difficult to penetrate the second through hole 611 and enter the tank body 1 space above the mounting plate 61 again, which can reduce the inhibitory effect of glycerol on the hydrolysis reaction, and further promote the forward reaction of hydrolysis, and improve the reaction rate and reaction equilibrium conversion rate. The product discharge pipe 32 passes through the mounting plate 61 through the long strip opening 614 and extends into the bottom of the tank body 1, which can discharge the heavy phase of glycerol first and promote the continuous progress of the hydrolysis reaction.

[0039] In this embodiment, an observation window 11 is installed on the outer wall of the tank body 1 to facilitate observation and adjustment of the position of the product discharge pipe 32 extending into the tank body 1, and also to facilitate observation of the deposition of the heavy phase of glycerol after hydrolysis so as to extract glycerol in time.

[0040] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may be subject to various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. An enzyme hydrolysis device, characterized in that: The invention comprises a tank body (1), wherein the tank body (1) is provided with a sealing cover (2), a material inlet and outlet assembly (3), a material mixing assembly (4) and a heating assembly (5), wherein the sealing cover (2) is movably connected to the top of the tank body (1); The inner cavity of the tank body (1) is provided with an enzyme loading assembly (6), and the enzyme loading assembly (6) comprises a mounting plate (61) and a plurality of enzyme loading columns (62). The mounting plate (61) is mounted in the inner cavity of the tank body (1), and the bottoms of the plurality of enzyme loading columns (62) are detachably connected to the surface of the mounting plate (61), and the wall surface of the enzyme loading columns (62) is provided with a plurality of first through holes (621).

2. The enzymatic hydrolysis device according to claim 1, characterized in that: The mixing component (4) comprises a speed regulating oscillator (41), an oscillation frame (42) and a plurality of oscillation springs (43), one end of each of the plurality of oscillation springs (43) being detachably connected to the top of the speed regulating oscillator (41), and the other end of each of the plurality of oscillation springs (43) being detachably connected to the bottom of the oscillation frame (42), and the tank body (1) being placed in the oscillation frame (42).

3. The enzymatic hydrolysis device according to claim 1, characterized in that: The enzyme loading column (62) is provided with a top cover (622), and a pull ring (623) is provided at the top of the top cover (622).

4. The enzymatic hydrolysis device according to claim 1, characterized in that: The surface of the mounting plate (61) is provided with a plurality of mounting holes (612), the mounting holes (612) are provided with internal threads (613), the bottom of the enzyme loading column (62) is provided with external threads (624), and the internal threads (613) and the external threads (624) cooperate with each other.

5. The enzymatic hydrolysis device according to claim 2, characterized in that: A pair of limit rods (44) are arranged in the oscillation frame (42), and the limit rods (44) are arranged on both sides of the tank body (1). One end of the limit rod (44) is detachably connected to the inner wall surface of the oscillation frame (42), and the other end of the limit rod (44) is detachably connected to the outer wall surface of the tank body (1).

6. The enzymatic hydrolysis device according to claim 1, characterized in that: The heating assembly (5) comprises a jacket (51) and an electric heating rod (52); the jacket (51) is arranged on the outer wall surface of the tank body (1); and the electric heating rod (52) is arranged in a receiving space formed between the jacket (51) and the tank body (1).

7. The enzymatic hydrolysis device according to any one of claims 1 to 6, characterized in that: The feed and discharge assembly (3) comprises a raw material feed pipe (31) and a product discharge pipe (32), wherein the raw material feed pipe (31) and the product discharge pipe (32) are both connected to the top of the sealing cover (2), and the raw material feed pipe (31) and the product discharge pipe (32) are both equipped with a control valve and a liquid pump.

8. The enzymatic hydrolysis device according to claim 7, characterized in that: The mounting plate (61) is mounted at a position close to the bottom of the tank body (1), and the mounting plate (61) is provided with a plurality of second through holes (611) and a long opening (614), and the product discharge pipe (32) passes through the mounting plate (61) through the long opening (614).

9. The enzymatic hydrolysis device according to claim 7, characterized in that: An observation window (11) is installed on the outer wall surface of the tank body (1).

Images