Chromatographic column
By using the inner layer made of inert materials and the outer layer made of metal materials in the chromatographic column, the problems of insufficient chemical stability and structural strength in high temperature and high pressure environments are solved, and better peak output effect and service life are achieved.
Patent Information
- Application Number
- CN202421538116.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-01
- Publication Date
- 2025-05-09
- Estimated Expiration
- 2034-07-01
AI Technical Summary
Existing chromatographic columns have poor chemical stability in high temperature and high pressure environments, and are prone to metal ions precipitation, affecting the peaking effect; at the same time, insufficient structural strength may lead to deformation of the column and affect performance.
An inner layer made of an inert material and an outer layer made of a metal material form a channel defined by the inner layer, the inner wall is composed of an inert material, and the outer layer provides structural strength.
It improves the chemical stability of the chromatographic column, prevents metal ions from precipitating, and improves peak output effect; at the same time, it enhances the structural strength of the column and extends the service life.
Smart Images
Figure CN222850574U_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the technical field of chromatography, and in particular to a chromatography column. Background Art
[0002] The contents of this section merely provide background information related to the present application and may not constitute prior art.
[0003] High performance liquid chromatography (HPLC) is a separation and analysis technology developed in the late 1960s and is an important means of modern separation and determination. Since its advent, HPLC has been widely used in biochemistry, medicine and clinical analysis due to its high separation efficiency, fast analysis speed, good detection sensitivity, and the ability to analyze thermally unstable physiologically active substances with high boiling points but not vaporized.
[0004] Chromatographic columns are the heart of HPLC. In the use of HPLC, it is very important to maintain the column efficiency, capacity and permeability of the chromatographic columns and extend their service life. Although after decades of development, a variety of special chromatographic columns have emerged, such as preparative columns, analytical columns and various micro columns, the main body of the chromatographic column is still composed of the column body, column filler and other parts.
[0005] In the related art, the body of a common chromatography column is generally made of metal (eg, stainless steel) or plastic (eg, PEEK).
[0006] As for chromatographic columns using metal columns, although they have good performance in working environments such as high temperature and high pressure, the chemical stability of such chromatographic columns is poor. In actual application, metal ions may precipitate and react with samples flowing through the column, thereby affecting the peak elution effect of the chromatographic column; at the same time, when the sample contains alkaline compounds, the metal ions will form a secondary retention effect on the alkaline compounds, thereby affecting the morphology of the chromatographic peak.
[0007] As for chromatographic columns using plastic columns, although they have good chemical stability and no ion precipitation, the structural strength of such columns is poor, so that the columns may deform in high temperature, high pressure and other working environments, thereby affecting the performance of the chromatographic columns. Utility Model Content
[0008] In view of this, the purpose of the present application is to provide a chromatographic column having both good chemical stability and structural strength, so as to improve the performance and service life of the chromatographic column.
[0009] The purpose of this application is achieved through the following technical solutions:
[0010] The present application discloses a chromatographic column, comprising:
[0011] A column having a first end and a second end opposite to each other; the column comprising an inner layer extending between the first end and the second end and an outer layer surrounding the inner layer, the inner layer defining a channel suitable for accommodating a filler, the inner wall of the channel comprising an inert material, and the outer layer being made of a metal material;
[0012] Two connectors are respectively arranged at the first end and the second end.
[0013] In some possible embodiments, the inner layer is made of the inert material.
[0014] In some possible embodiments, the inert material is glass, ceramic or sapphire.
[0015] In some possible embodiments, a filling layer is provided between the inner layer and the outer layer.
[0016] In some possible embodiments, the filling layer is epoxy resin.
[0017] In some possible embodiments, the channel has two drainage ports located at the first end and the second end respectively, and the two drainage ports are both connected to the channel;
[0018] The connector comprises:
[0019] Screen plate;
[0020] A sieve plate cap is configured to position the sieve plate at the corresponding drainage port; the sieve plate cap defines a first drainage channel aligned with the sieve plate;
[0021] A locking member is detachably connected to the column; the locking member is configured to lock or unlock the screen plate cap.
[0022] In some possible embodiments, the sieve plate cap is provided with an inserting groove adapted to the end portion corresponding to the inner layer, the innermost side of the inserting groove is provided with a receiving groove suitable for receiving the sieve plate, and the first drainage channel is connected to the receiving groove.
[0023] In some possible embodiments, the locking member is provided with a connecting groove adapted to the end portion corresponding to the outer layer, the innermost side of the connecting groove is provided with a limiting groove adapted to the sieve plate cap, and the locking member is provided with a second drainage channel connected to the limiting groove, and the second drainage channel is aligned with and connected to the first drainage channel.
[0024] In some possible embodiments, the accommodating groove is provided with an arc-shaped guide wall, the guide wall is recessed in a direction away from the sieve plate, and the first drainage channel is connected to a position of the guide wall farthest from the sieve plate.
[0025] In some possible embodiments, the metal material is titanium or stainless steel.
[0026] The technical solution of the embodiment of the present application has at least the following advantages and beneficial effects:
[0027] The chromatographic column disclosed in the present application uses an inert material to form the inner wall of the channel defined by the inner layer, so that the inner wall of the channel has good chemical stability, so that when separating samples, it can effectively prevent the inner wall of the channel from having adverse phenomena such as metal ion precipitation, thereby making the chromatographic column have a good peak effect and improving the column efficiency of the chromatographic column. At the same time, by using an outer layer made of a metal material to surround the inner layer, the structural strength of the entire column can be effectively improved, so that the chromatographic column still has good performance when used in working environments such as high temperature and high pressure, and the service life of the chromatographic column is improved. BRIEF DESCRIPTION OF THE DRAWINGS
[0028] Figure 1 A schematic diagram of the structure of a chromatographic column provided in an embodiment of the present application;
[0029] Figure 2 for Figure 1 A cross-sectional view of a chromatography column is shown;
[0030] Figure 3 A schematic diagram of the structure of a column provided in an embodiment of the present application;
[0031] Figure 4 A partial structural cross-sectional view of a column provided in an embodiment of the present application;
[0032] Figure 5 for Figure 4 The enlarged view of point A in the middle;
[0033] Figure 6 for Figure 2 The enlarged view of point B in the middle;
[0034] Figure 7 for Figure 6 A schematic diagram of the structure of the sieve plate cap shown in FIG.
[0035] Figure 8 for Figure 6 A schematic structural diagram of a locking member shown in FIG.
[0036] Fig. 9 for Figure 6 Enlarged view of point C in the middle.
[0037] Icon: 10-column, 11-first end, 12-second end, 13-inner layer, 14-outer layer, 15-channel, 16-drainage port, 17-filling layer, 20-connector, 21-sieve plate, 22-sieve plate cap, 221-first drainage channel, 222-plug-in slot, 223-accommodating slot, 2231-guide wall, 23-locking piece, 231-second drainage channel, 232-connecting slot, 233-limiting slot. DETAILED DESCRIPTION
[0038] In order to make the purpose, technical solutions and advantages of the present application clearer, the technical solutions of the present application will be clearly and completely described below in conjunction with specific implementation methods. The same figure marks in the accompanying drawings represent the same components. It should be noted that the described embodiments are part of the embodiments of the present application, rather than all of the embodiments. Based on the described embodiments of the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.
[0039] Compared to the embodiments shown in the drawings, feasible implementations within the scope of protection of the present application may have fewer components, other components not shown in the drawings, different components, differently arranged components, or differently connected components, etc. In addition, two or more components in the drawings may be implemented in a single component, or a single component shown in the drawings may be implemented as multiple separate components.
[0040] Unless otherwise defined, the technical or scientific terms used herein shall have the ordinary meanings understood by persons with ordinary skills in the field to which this application belongs. The words "first", "second" and similar words used in the specification and claims of this application do not indicate any order, quantity or importance, but are only used to distinguish different components.
[0041] like Figure 1 and Figure 2 As shown, it shows the general structure of the exemplary chromatographic column disclosed in the embodiment of the present application. Figure 1 The appearance of the chromatographic column is shown. Figure 2 Generally speaking, the chromatographic column disclosed in the embodiments of the present application may include a column body 10 and two joints 20 .
[0042] Combination Figure 3 and Figure 4As shown, the column 10 is generally tubular and has a first end 11 and a second end 12. At the same time, the column 10 may include an inner layer 13 extending between the first end 11 and the second end 12 and an outer layer 14 surrounding the inner layer 13. In the embodiment of the present application, both the inner layer 13 and the outer layer 14 may be tubular, and the inner layer 13 is surrounded by the outer layer 14, that is, the outer layer 14 is sleeved outside the inner layer 13.
[0043] Further, the inner layer 13 defines a channel 15 suitable for accommodating fillers, and the channel 15 has two drainage ports 16 respectively located at the first end 11 and the second end 12, and the two drainage ports 16 are both connected to the channel 15. It can be understood that the two drainage ports 16 provided at both ends of the channel 15 are respectively used for allowing external samples to enter the channel 15 and for allowing the sample flowing through the channel 15 to flow out of the channel 15. In other words, the external sample can enter the channel 15 through one of the drainage ports 16 (for example, the drainage port 16 located at the first end 11), and can flow out from the other drainage port 16 (for example, the drainage port 16 located at the second end 12) after flowing through the channel 15 and being separated by the fillers in the channel 15. Among them, the fillers filled in the channel 15 can be fillers used in chromatographic columns known in the prior art, and the type of fillers is not limited here.
[0044] In addition, the inner wall of the channel 15 (i.e., the wall of the channel 15 that contacts the sample after the sample enters the channel 15) includes an inert material. It is understood that by using an inert material to form the inner wall of the channel 15, the inner wall of the channel 15 can have good chemical stability, thereby effectively preventing the inner wall of the channel 15 from having adverse phenomena such as metal ion precipitation when separating the sample, thereby making the chromatographic column have a good peak effect and avoiding the morphology of the chromatographic peak from being affected, so as to improve the column efficiency of the chromatographic column.
[0045] The above-mentioned inert material can be glass, ceramic or sapphire. Such inert materials can provide good dimensional stability while providing good chemical stability, which is convenient for the processing and manufacturing of the chromatographic column. In addition, in some embodiments of the present application, the entire inner layer 13 can be made of inert materials to further reduce the difficulty of processing and manufacturing the chromatographic column. For example, the inner layer 13 can be a glass tube, a ceramic tube or a sapphire tube.
[0046] On this basis, the outer layer 14 surrounding the inner layer 13 mentioned above can be made of a metal material. It is understandable that by using the outer layer 14 made of a metal material to surround the inner layer 13, based on the good structural strength of the metal material, the structural strength of the entire column 10 can be effectively improved, so that the chromatographic column still has good performance when used in working environments such as high temperature and high pressure, and abnormal conditions such as deformation of the column 10 will not easily occur, so as to improve the service life of the chromatographic column. Among them, the metal material can be titanium or stainless steel. For example, the outer layer 14 can be a titanium tube or a stainless steel tube.
[0047] like Figure 5 As shown, the column 10 in the embodiment of the present application may further include a filling layer 17 disposed between the inner layer 13 and the outer layer 14. It is understandable that, by providing the filling layer 17, on the one hand, a gap between the inner layer 13 and the outer layer 14 can be avoided, thereby further improving the structural strength of the column 10, and on the other hand, the filling layer 17 can be used to effectively bond the inner layer 13 and the outer layer 14 together, thereby forming an integrated structure of the column 10. Among them, the filling layer 17 can be epoxy resin. Of course, in other embodiments of the present application, the filling layer 17 can also be glue or other thermosetting plastics.
[0048] In the embodiments of the present application, Figure 1 and Figure 2 As shown in the figure, two connectors 20 are respectively arranged at the first end 11 and the second end 12 of the column 10. The two connectors 20 are respectively used to guide the external sample into the channel 15 and the sample flowing through the channel 15 to flow out of the channel 15, and the two connectors 20 can at least provide the function of filtering the sample entering the channel 15 and the sample flowing out of the channel 15.
[0049] For ease of explanation, the embodiment of the present application defines the drainage port 16 located at the first end 11 as an inlet for the sample to enter the channel 15, and defines the drainage port 16 located at the second end 12 as an outlet for the sample to flow out of the channel 15. At the same time, based on the fact that the two connectors 20 in the embodiment of the present application have a substantially identical structure, the structure of the connector 20 will be described in detail below by taking one connector 20 as an example.
[0050] Combination Figure 6As shown in the figure, the joint 20 may include a sieve plate 21, a sieve plate cap 22 and a locking member 23. The sieve plate 21 may be disposed on the sieve plate cap 22, so that the sieve plate 21 is positioned at the corresponding drainage port 16 through the sieve plate cap 22. At the same time, the sieve plate cap 22 also defines a first drainage channel 221 aligned with the sieve plate 21. The locking member 23 is detachably connected to the column 10, so that the sieve plate cap 22 is locked or unlocked by the locking member 23, that is, the sieve plate cap 22 is fixed or released by the locking member 23.
[0051] For example, for the connector 20 corresponding to the flow inlet 16 as the injection port, the sieve plate cap 22 of the connector 20 can position the sieve plate 21 at the flow inlet 16 as the injection port, and the locking member 23 of the connector 20 can be connected to the column 10 to lock the sieve plate cap 22, thereby temporarily fixing the sieve plate cap 22 and the sieve plate 21 of the connector 20. In this way, when the chromatographic column is used, the external sample can flow through the first flow inlet 221 of the connector 20 and the sieve plate 21 in sequence and then enter the channel 15 from the flow inlet 16 as the injection port.
[0052] It can be understood that by providing a removable sieve plate cap 22 to locate the position of the sieve plate 21, and by using a detachable locking piece 23 to lock or unlock the sieve plate cap 22, the sieve plate cap 22 and the sieve plate 21 are both easily replaceable components, thereby facilitating replacement of the sieve plate 21 of different specifications and materials according to usage requirements.
[0053] In order to simplify the process of positioning the sieve plate 21 using the sieve plate cap 22 as much as possible, Figure 7 As shown, the sieve plate cap 22 of a single joint 20 can be provided with a plug-in groove 222 adapted to the end corresponding to the inner layer 13. At this time, the innermost side of the plug-in groove 222 is also provided with a receiving groove 223 suitable for accommodating the sieve plate 21, and the first drainage channel 221 is connected with the receiving groove 223. For example, for the joint 20 provided at the first end 11, when it is necessary to position the sieve plate 21 of the joint 20 at the drainage port 16 located at the first end 11 and serving as the injection port, the sieve plate 21 can be first placed in the receiving groove 223 of the sieve plate cap 22 to temporarily fix the sieve plate 21 on the sieve plate cap 22, and then the plug-in groove 222 of the sieve plate cap 22 can be aligned with the end corresponding to the inner layer 13, so that the sieve plate cap 22 is plugged into the inner layer 13 to achieve temporary fixation of the sieve plate cap 22, thereby achieving the purpose of positioning the sieve plate 21 at the drainage port 16 serving as the injection port by using the sieve plate cap 22, and the operation is simple and convenient.
[0054] In the embodiment of the present application, for a single joint 20, the above-mentioned detachable connection between the locking piece 23 and the column 10 can further be a detachable connection between the locking piece 23 and the outer layer 14. Since the outer layer 14 is made of metal material, the detachable connection between the locking piece 23 and the outer layer 14 can effectively improve the connection strength between the two.
[0055] Specifically, Figure 8 As shown, the locking piece 23 is provided with a connecting groove 232 adapted to the end corresponding to the outer layer 14, wherein the connecting groove 232 can be an internal threaded hole with an internal thread, so that the end corresponding to the outer layer 14 can be inserted into the connecting groove 232 and threadedly connected to the connecting groove 232.
[0056] At the same time, the innermost side of the connection groove 232 is provided with a limiting groove 233 adapted to the sieve plate cap 22. In addition, the locking member 23 is provided with a second drainage channel 231 connected to the limiting groove 233, and the second drainage channel 231 can be aligned with and connected to the first drainage channel 221 on the sieve plate cap 22. In this way, for a single joint 20, when the sieve plate cap 22 is temporarily plugged into the inner layer 13 in a plug-in manner to position the sieve plate 21 at the corresponding drainage port 16, the connection groove 232 of the locking member 23 is aligned with the corresponding end of the outer layer 14, so that the corresponding end of the outer layer 14 is threadedly connected to the connection groove 232. At this time, the sieve plate cap 22 will be located in the limiting groove 233 of the locking member 23 and limited by the locking member 23, so as to lock the position of the sieve plate cap 22, and the first drainage channel 221 on the sieve plate cap 22 is just aligned with and connected to the second drainage channel 231 on the locking member 23.
[0057] In this way, for the connector 20 arranged at the first end 11 of the column 10, the external sample can flow through the second drainage channel 231, the first drainage channel 221 and the corresponding sieve plate 21 in sequence and then enter the channel 15 from the drainage port 16 serving as the sample inlet; correspondingly, for the connector 20 arranged at the second end 12 of the column 10, the sample that flows through the channel 15 and is separated will flow out from the drainage port 16 serving as the sample outlet, and flow through the sieve plate 21, the first drainage channel 221 and the second drainage channel 231 of the connector 20 in sequence and then be discharged.
[0058] Furthermore, the locking member 23 may be a locking nut that can be threadedly connected to the outer layer 14 to enhance the structural strength and wear resistance of the joint 20 .
[0059] In addition, combined Figure 7 and Fig. 9As shown in the figure, the receiving groove 223 may be provided with an arc-shaped guide wall 2231, and the guide wall 2231 is recessed in the direction away from the sieve plate 21, at which time, the first guide channel 221 on the sieve plate cap 22 is connected to the position of the guide wall 2231 farthest from the sieve plate 21. In this way, for the connector 20 provided at the first end 11 of the column 10, when the external sample flows out through the first guide channel 221 of the connector 20, under the guiding effect of the guide wall 2231, the sample can be quickly diffused to various areas of the sieve plate 21 of the connector 20, thereby facilitating the external sample to quickly enter the channel 15 through the guide port 16 serving as the sample inlet. Correspondingly, for the connector provided at the second end 12 of the column 10, when the sample in the channel 15 flows through the drainage port 16 serving as the sample outlet and the sieve plate 21 of the connector 20 in sequence, the sample can quickly converge to the first drainage channel 221 of the connector 20 under the guiding action of the guide wall 2231, thereby accelerating the discharge of the sample.
[0060] The above are only preferred embodiments of the present application and are not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Claims
1. A chromatographic column, characterized in that include: A column having a first end and a second end opposite to each other; the column comprising an inner layer extending between the first end and the second end and an outer layer surrounding the inner layer, the inner layer defining a channel suitable for accommodating a filler, the inner wall of the channel comprising an inert material, and the outer layer being made of a metal material; Two connectors are respectively arranged at the first end and the second end.
2. The chromatographic column according to claim 1, characterized in that The inner layer is made of the inert material.
3. The chromatographic column according to claim 1 or 2, characterized in that The inert material is glass, ceramic or sapphire.
4. The chromatographic column according to claim 1, characterized in that A filling layer is provided between the inner layer and the outer layer.
5. The chromatographic column according to claim 4, characterized in that The filling layer is epoxy resin.
6. The chromatographic column according to claim 1, characterized in that The channel has two drainage ports located at the first end and the second end respectively, and both of the drainage ports are connected to the channel; The connector comprises: Screen plate; A sieve plate cap is configured to position the sieve plate at the corresponding drainage port; the sieve plate cap defines a first drainage channel aligned with the sieve plate; A locking member is detachably connected to the column; the locking member is configured to lock or unlock the screen plate cap.
7. The chromatographic column according to claim 6, characterized in that The sieve plate cap is provided with an inserting groove adapted to the end portion corresponding to the inner layer, the innermost side of the inserting groove is provided with a receiving groove suitable for receiving the sieve plate, and the first drainage channel is connected to the receiving groove.
8. The chromatographic column according to claim 7, characterized in that The locking piece is provided with a connecting groove adapted to the end portion corresponding to the outer layer, the innermost side of the connecting groove is provided with a limiting groove adapted to the sieve plate cap, the locking piece is provided with a second drainage channel connected to the limiting groove, and the second drainage channel is aligned with and connected to the first drainage channel.
9. The chromatographic column according to claim 7, characterized in that The receiving groove is provided with an arc-shaped guide wall, the guide wall is recessed in a direction away from the sieve plate, and the first drainage channel is communicated with a position of the guide wall farthest from the sieve plate.
10. The chromatographic column according to claim 1, characterized in that The metal material is titanium or stainless steel.