Trace plant specimen sample DNA adsorption and collection device

Abstract

The utility model discloses a trace plant specimen DNA (deoxyribonucleic acid) adsorption and collection device, which belongs to the technical field of molecular biology and comprises a base, a grinding bowl is fixedly connected to the upper end of the base, and a first blanking hole and a second blanking hole are respectively formed in the bottom end of the inner wall of the grinding bowl. According to the scheme, powdery plant tissue is pushed into the second collecting tank or the first collecting tank from the interior of the grinding bowl, then powder in the powdery plant tissue is poured into the centrifugal tube at a time to be collected, and compared with the prior art that a digging spoon is used for transferring for multiple times, the powder collecting device has the advantages that the powder collecting device is simple in structure and convenient to use. Compared with the prior art, the extraction device has the advantages that the extraction speed is obviously increased, the retention time of plant tissues in air can be effectively shortened, the extraction speed is increased, the degradation degree of DNA (deoxyribonucleic acid) is reduced, the amount of extracted powder can be accurately controlled within two intervals of 50-200mg and 15-50mg through the second collection tank and the first collection tank, and convenience is brought to extraction work.

Description

Technical Field

[0001] The utility model relates to the field of molecular biotechnology, and more specifically to a device for collecting DNA adsorption of trace plant specimen samples. Background Art

[0002] DNA is the carrier of genetic information and the basic genetic material. It plays an important role in genetic variation, metabolic regulation, etc. It is the object of molecular biology and genetic engineering research. However, whether it is to study the structure and function of plant DNA or to carry out research on the transformation and transduction of exogenous DNA, the first thing to do is to extract high-molecular-weight purified DNA in its natural state from plant tissues.

[0003] Among the steps of extracting plant DNA, one of them is to use a centrifuge tube to collect powder of plant tissue. The user needs to put the plant tissue into a grinding mortar and add liquid nitrogen into the grinding mortar. The plant tissue is ground into powder while the liquid nitrogen is submerged. Then, an appropriate amount of powdered plant tissue is weighed into a 1.5m centrifuge tube pre-cooled with liquid nitrogen. The DNA concentration in the plant tissue may vary depending on the type, part and treatment method of the plant. A higher concentration DNA sample may require a smaller amount of powder, while a lower concentration DNA sample may require a larger amount of powder, which is generally divided into two ranges: 50-200mg and 15-50mg.

[0004] The process of transferring the ground powdered plant tissue to the centrifuge bottle must be completed quickly. This is because after the plant tissue is broken, the nuclease in the cells will begin to degrade the DNA. If the tissue is exposed to the environment for a long time, the DNA quality may be further degraded, thereby affecting the subsequent DNA extraction and analysis. In the prior art, users mostly transfer the powdered plant tissue in multiple times using a scooping spoon, which is slow and easily causes further DNA degradation. In addition, the amount of the extracted powdered plant tissue cannot be accurately determined by the scooping spoon, which is inconvenient to use. Therefore, a DNA adsorption and collection device for trace plant specimen samples is proposed to address the above problems. Utility Model Content

[0005] 1. Technical issues to be solved

[0006] In view of the problems existing in the prior art, the purpose of the utility model is to provide a device for adsorbing and collecting DNA of trace plant specimen samples. The present invention pushes the powdered plant tissue from the grinding bowl into a second collecting tank or a first collecting tank, and then pours the powder into a centrifuge tube for collection at one time. Compared with the prior art of using a scoop for multiple transfers, the speed is significantly improved, which can effectively reduce the time that the plant tissue stays in the air, speed up the extraction speed, and reduce the degree of DNA degradation. Moreover, the second collecting tank and the first collecting tank can accurately control the amount of extracted powder within the two ranges of 50-200 mg and 15-50 mg, which brings convenience to the extraction work.

[0007] 2. Technical solution

[0008] To solve the above problems, the utility model adopts the following technical solutions.

[0009] A device for collecting DNA adsorption of trace plant specimen samples comprises a base, a grinding bowl is fixedly connected to the upper end of the base, a first feeding hole and a second feeding hole are respectively provided at the bottom end of the inner wall of the grinding bowl, and a second collecting tank and a first collecting tank are respectively provided at the lower side of the grinding bowl, a limiting groove is provided on the base, the first feeding hole and the second feeding hole are both connected to the limiting groove, a baffle plate matching the limiting groove is provided in the limiting groove, and a through hole is provided on the baffle plate.

[0010] Furthermore, the first material discharge hole, the second material discharge hole and the through hole are arranged in a ring shape with the center point of the grinding bowl as the center, and the first material discharge hole and the second material discharge hole are set to have different inner diameters.

[0011] Furthermore, the outer end of the baffle is fixedly connected with a lever, and the lever extends to the outside of the grinding bowl.

[0012] Furthermore, a pair of mounting blocks are fixedly connected to the bottom end of the grinding bowl, and the pair of mounting blocks are respectively arranged on the outer sides of the first feeding hole and the second feeding hole.

[0013] Further, a pair of the mounting blocks are matched with the second collection tank and the first collection tank respectively.

[0014] Furthermore, the capacity range of the first collection tank is set between 15-50 mg, and the capacity range of the second collection tank is set between 50-200 mg.

[0015] 3. Beneficial effects

[0016] Compared with the prior art, the advantages of the present invention are:

[0017] In this solution, the powdered plant tissue is pushed from the grinding bowl into the second collection tank or the first collection tank, and the powder is poured into a centrifuge tube for collection at one time. Compared with the prior art of using a scoop for multiple transfers, the speed is significantly improved, which can effectively reduce the time that the plant tissue stays in the air, speed up the extraction speed, and reduce the degree of DNA degradation. In addition, the amount of powder extracted can be accurately controlled within the two ranges of 50-200 mg and 15-50 mg through the second collection tank and the first collection tank, which brings convenience to the extraction work. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0019] Figure 2 This is a schematic diagram of the internal structure of the grinding bowl of the utility model;

[0020] Figure 3 This is a schematic diagram of the partial explosion structure of the utility model;

[0021] Figure 4 It is a schematic diagram of the lower structure of the grinding bowl of the utility model.

[0022] Description of the numbers in the figure:

[0023] 1. Base; 2. Grinding bowl; 3. First feeding hole; 4. Second feeding hole; 5. Baffle; 6. Through hole; 7. Push rod; 8. Limiting groove; 9. Mounting block; 10. Second collecting tank; 11. First collecting tank. DETAILED DESCRIPTION

[0024] The technical solutions in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model; it is obvious that the described embodiments are only part of the embodiments of the utility model, rather than all of the embodiments, and all other embodiments obtained by ordinary technicians in this field based on the embodiments of the utility model without making creative work are within the scope of protection of the utility model.

[0025] In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "top / bottom" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific position, be constructed and operated in a specific position, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0026] In the description of the present utility model, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "provided with", "set / connected", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0027] Example:

[0028] See also Figure 1-3 A device for collecting DNA adsorption of trace plant specimen samples comprises a base 1, a grinding bowl 2 is fixedly connected to the upper end of the base 1, a first material discharge hole 3 and a second material discharge hole 4 are respectively provided at the bottom end of the inner wall of the grinding bowl 2, and a second collecting tank 10 and a first collecting tank 11 are respectively provided at the lower side of the grinding bowl 2, a limiting groove 8 is provided on the base 1, the first material discharge hole 3 and the second material discharge hole 4 are both connected to the limiting groove 8, a baffle 5 matching the limiting groove 8 is provided in the limiting groove 8, and a through hole 6 is provided on the baffle 5.

[0029] DNA is the carrier of genetic information and the basic genetic material. Whether it is to study the structure and function of plant DNA or to carry out research on the transformation and transduction of exogenous DNA, the first thing to do is to extract high-molecular-weight purified DNA in a natural state from plant tissues. In the steps of extracting plant DNA, one of them is to use a centrifuge tube to collect the powder of plant tissue. In this solution, the user can put the plant tissue into a grinding bowl 2, and add liquid nitrogen into the grinding bowl 2, and grind the plant tissue into powder when the liquid nitrogen submerges the plant tissue. Next, the user needs to use a 1.5m centrifuge tube pre-cooled with liquid nitrogen to quickly weigh an appropriate amount of powdered plant tissue. The DNA concentration in the plant tissue may vary depending on the type, part and treatment method of the plant. A DNA sample with a higher concentration may require a smaller amount of powder, while a DNA sample with a lower concentration may require a larger amount of powder, which is generally divided into two ranges of 50-200 mg and 15-50 mg. However, in the prior art, users mostly transfer the powdered plant tissue in multiple times by using a scooping spoon, which is slow and easy to further cause DNA degradation. In addition, the amount of the extracted powdered plant tissue cannot be accurately determined by the scooping spoon, which is inconvenient to use.

[0030] The first material discharge hole 3, the second material discharge hole 4 and the through hole 6 are arranged in a circle with the center point of the grinding bowl 2 as the center. The first material discharge hole 3 and the second material discharge hole 4 are set to have different inner diameters. The outer end of the baffle 5 is fixedly connected to a lever 7, and the lever 7 extends to the outside of the grinding bowl 2.

[0031] In the present embodiment, the baffle 5 is located inside the limiting groove 8, and the surface of the baffle 5 covers the first feed hole 3, so that the powder in the grinding bowl 2 will not fall into the first feed hole 3 or the second feed hole 4. After grinding the plant tissue into powder, the user can turn the lever 7 to drive the through hole 6 to rotate in the limiting groove 8 through the lever 7. By changing the rotation direction, the through hole 6 is selected to coincide with the first feed hole 3 or the second feed hole 4. With the user's simple turning of the powder, the powder can fall from the first feed hole 3 or the second feed hole 4. The inner diameters of the first feed hole 3 and the second feed hole 4 are different, which is convenient for the user to distinguish them.

[0032] See also Figure 4 A pair of mounting blocks 9 are fixedly connected to the bottom end of the grinding mortar 2, and the pair of mounting blocks 9 are respectively arranged on the outside of the first discharge hole 3 and the second discharge hole 4. The pair of mounting blocks 9 are respectively matched with the second collection tank 10 and the first collection tank 11. The capacity range of the first collection tank 11 is set between 15-50 mg, and the capacity range of the second collection tank 10 is set between 50-200 mg.

[0033] The second collecting tank 10 and the first collecting tank 11 can be respectively clamped in a pair of mounting blocks 9, so that the powder dropped from the first discharge hole 3 or the second discharge hole 4 can fall into the corresponding second collecting tank 10 or the first collecting tank 11, wherein the first discharge hole 3 corresponds to the first collecting tank 11, and the second discharge hole 4 corresponds to the second collecting tank 10. The second collecting tank 10 and the first collecting tank 11 can be set to transparent materials. In the process of the powder entering the second collecting tank 10 or the first collecting tank 11, the user can clearly observe the amount of powder. When the powder is almost filling the second collecting tank 10 or the first collecting tank 11, the user can rotate the baffle 5 to reset it, so that it can re-block the first discharge hole 3 and the second discharge hole 4, and then remove the second collecting tank 10 or the first collecting tank 11, and then collect the powder therein. The powder can be directly poured into the centrifuge tube. With this arrangement, the user can choose the second collecting tank 10 or the first collecting tank 11 to fill the powder according to the plant type and the required amount of powder, so as to ensure the correct amount of powder. In this solution, the user only needs to push the powder in the grinding bowl 2 into the first discharge hole 3 or the second discharge hole 4, and then pour the powder in the second collecting tank 10 or the first collecting tank 11 into the centrifuge tube at one time. Compared with the prior art of using a scoop for multiple transfers, the speed is significantly improved, which can reduce the time that plant tissue stays in the air, speed up the extraction speed, and reduce the degree of DNA degradation. The second collecting tank 10 and the first collecting tank 11 can accurately control the amount of extracted powder within the two ranges of 50-200 mg and 15-50 mg, which brings convenience to the extraction work.

[0034] Working principle:

[0035] The user puts the plant tissue into the grinding bowl 2, and adds liquid nitrogen into the grinding bowl 2, and grinds the plant tissue into powder when the liquid nitrogen submerges the plant tissue. Then the user can turn the lever 7 to drive the through hole 6 to rotate in the limiting groove 8, and select the through hole 6 to coincide with the first feeding hole 3 or the second feeding hole 4 by changing the rotation direction. With the user's simple turning of the powder, the powder can fall from the first feeding hole 3 or the second feeding hole 4, and the powder falling from the first feeding hole 3 or the second feeding hole 4 can fall into the corresponding second collecting tank 10 or the first collecting tank 11, wherein the first feeding hole 3 and the first collecting hole 4 are respectively The collecting tank 11 corresponds to the second discharge hole 4 and the second collecting tank 10. When the powder enters the second collecting tank 10 or the first collecting tank 11, the user can clearly observe the amount of powder. When the powder is almost filling the second collecting tank 10 or the first collecting tank 11, the user can rotate the baffle 5 to reset it so that it can re-block the first discharge hole 3 and the second discharge hole 4, and then remove the second collecting tank 10 or the first collecting tank 11, and then pour the powder therein directly into the centrifuge tube. With this arrangement, the amount of extracted powder can be accurately controlled within the two ranges of 50-200 mg and 15-50 mg.

[0036] The above is only a preferred specific implementation of the utility model; however, the protection scope of the utility model is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical solution and improved ideas of the utility model within the technical scope disclosed by the utility model, which should be included in the protection scope of the utility model.

Claims

1. A device for collecting DNA from trace plant specimens by adsorption, comprising a base (1), characterized in that: A grinding bowl (2) is fixedly connected to the upper end of the base (1), a first material discharge hole (3) and a second material discharge hole (4) are respectively provided at the bottom end of the inner wall of the grinding bowl (2), and a second collecting tank (10) and a first collecting tank (11) are respectively provided on the lower side of the grinding bowl (2), a limiting groove (8) is provided on the base (1), the first material discharge hole (3) and the second material discharge hole (4) are both connected to the limiting groove (8), a baffle (5) matching the limiting groove (8) is provided in the limiting groove (8), and a through hole (6) is provided on the baffle (5).

2. The device for collecting DNA from trace plant specimens according to claim 1, characterized in that: The first material discharge hole (3), the second material discharge hole (4) and the through hole (6) are arranged in a ring shape with the center point of the grinding bowl (2) as the center of the circle, and the first material discharge hole (3) and the second material discharge hole (4) are set to have different inner diameters.

3. The device for collecting DNA from trace plant specimens according to claim 1, characterized in that: The outer end of the baffle (5) is fixedly connected to a lever (7), and the lever (7) extends to the outside of the grinding bowl (2).

4. The device for collecting DNA from trace plant specimens according to claim 1, characterized in that: A pair of mounting blocks (9) are fixedly connected to the bottom end of the grinding bowl (2), and the pair of mounting blocks (9) are respectively arranged on the outside of the first material discharge hole (3) and the second material discharge hole (4).

5. The device for collecting DNA from trace plant specimens according to claim 4, characterized in that: A pair of mounting blocks (9) are matched with the second collection tank (10) and the first collection tank (11) respectively.

6. The device for collecting DNA from trace plant specimens according to claim 1, characterized in that: The capacity range of the first collection tank (11) is set between 15 and 50 mg, and the capacity range of the second collection tank (10) is set between 50 and 200 mg.

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