Single-cell suspension preparation device

Abstract

The utility model belongs to the technical field of cell suspension preparation, and particularly relates to a single-cell suspension preparation device which comprises a shell, a base, a motor assembly, a dissociation device and a heating device, the motor assembly is fixed with the base, and the shell covers the base; the motor assembly comprises at least one rotatable motor, the dissociation device is installed above the motor and connected with the output end of the motor, the heating device is arranged outside the dissociation device in a sleeving mode and clamped on the motor assembly, a control system is further arranged on one side of the shell, and the motor assembly and the heating device are both electrically connected with the control system. According to the utility model, a plurality of groups of dissociation devices are respectively arranged on the motors which can be independently controlled, so that a plurality of groups of single-cell suspensions can be automatically prepared while the temperature is constant, and the device is small in size, simple in structure, convenient to disassemble, short in time consumption and high in efficiency.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell suspension preparation, and in particular relates to a single cell suspension preparation device. Background Art

[0002] Single cell suspension refers to a suspension in which single cells are dispersed in culture medium or other balanced salt solutions or buffer solutions. The preparation method is to digest and beat adherent cells, cell clusters, and suspension cells to disperse the adherent cells. Adherent cells generally need to be digested and detached before being beaten. Cells that are suspended cultures do not need to be digested and can be beaten directly.

[0003] The preparation of single-cell suspension is an important method for preparing samples for cell molecular biology experiments. Single-cell suspension is used in hundreds of experimental operations such as cell flow cytometry, plasmid extraction, gel recovery, PCR product purification, genome extraction, RNA separation and purification, PCR / RT-PCR, fluorescence quantification, cloning vectors and competent cell preparation. When preparing single-cell suspension, the first thing to look at is the sample type. For cells that are already in suspension, cells enriched with magnetic beads or cells sorted by flow cytometry, only washing and counting are required. When processing tissue samples, they must first be dissociated by mechanical or enzymatic methods. In order to achieve the best processing effect, the experimental method needs to be optimized multiple times.

[0004] The existing technology is to prepare single-cell suspension by manual operation, and the main operation steps include: first, sampling pretreatment is performed, that is, small pieces of tissue of 2-4 mm are taken with scissors or a knife; then separation treatment is performed, and the tissue is decomposed into a single-cell suspension by shearing, grinding or enzymatic hydrolysis for subsequent experimental operations. Utility Model Content

[0005] The utility model aims to overcome the deficiencies of the prior art and provide a single cell suspension preparation device, which can automatically prepare multiple groups of single cell suspensions while keeping the temperature constant, has a small size, a simple structure, is easy to disassemble, takes a short time and is highly efficient.

[0006] To achieve the above-mentioned purpose, the technical solution adopted by the utility model is: a single-cell suspension preparation device, including a shell, a base, a motor assembly, a dissociation device and a heating device, the motor assembly is fixed to the base, and the shell cover is arranged on the base; the motor assembly includes at least one rotatable motor, the dissociation device is installed above the motor and connected to the output end of the motor, the heating device is sleeved outside the dissociation device and clamped on the motor assembly, and a control system is also provided on one side of the shell, and the motor assembly and the heating device are electrically connected to the control system.

[0007] Preferably, the motor assembly includes a bracket, a motor and a coupling. The bottom of the bracket is fixed to the base. The motor can be arranged on the bracket in a forward and reverse manner. The output end of the motor is arranged to pass through the top of the bracket. The dissociation device is connected to the output end of the motor through a coupling. At least one positioning block is also provided on the top of the bracket, and the heating device is docked with the positioning block.

[0008] Preferably, 2 to 4 motors are provided, and each of the motors is electrically connected to the control system.

[0009] Preferably, the dissociation device includes a dissociation seat, a dissociation tube and a rotating assembly, the dissociation seat is sleeved outside the rotating assembly, and the bottom is fixed to the motor assembly, one end of the rotating assembly is connected to the motor assembly, and the other end is in contact with the outer surface of the dissociation seat; the detachable cover of the dissociation tube is arranged on the dissociation seat.

[0010] Preferably, the rotating assembly comprises a rotating shaft, one end of which is connected to the motor assembly, and the other end of which is provided with at least one set of rotating cutter heads, wherein the rotating cutter heads are in contact with the outer edge surface of the dissociation seat.

[0011] Preferably, the heating device includes a heating shell, on which mounting holes are evenly arranged, and the number of mounting holes is adapted to the number of motors. The dissociation device is installed above the motor, and the heating shell cover is arranged outside the dissociation device; a positioning groove is opened on the outer ring of the heating device, and the heating device is clamped on the motor assembly through the positioning groove; and a plurality of heating tubes are also evenly arranged in the heating shell.

[0012] Preferably, the control system includes a control panel and a display screen, wherein the control panel is mounted on the base and the display screen is embedded on the front side of the housing.

[0013] Preferably, it also includes a dustproof mechanism, which is hinged on the shell.

[0014] Preferably, the dustproof mechanism comprises a dustproof cover which is hinged on the outer shell, a handle is provided on the outside of the dustproof cover, a buffer column is provided inside the dustproof cover, one end of the buffer column is fixed in the dustproof cover, and the other end is in contact with the top of the outer shell.

[0015] Preferably, the dust cover is hinged to the housing via a damping hinge.

[0016] Compared with the prior art, the above technical solution has the following beneficial effects:

[0017] 1. The utility model sets the heating device outside the dissociation device, and controls the temperature of the heating device to be constant through the control system. The dissociation device at a constant temperature grinds the sample through the rotation of the motor.

[0018] 2. The utility model is equipped with multiple motors, and the multiple motors can be controlled independently, so that multiple groups of single cell suspensions can be automatically prepared while the temperature is constant, which is time-saving and efficient.

[0019] 3. The utility model has a simple structure, is easy to disassemble, occupies little space and is easy to carry. The design of the dust cover can effectively prevent dust from entering when the device is not in use. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a cross-sectional view of the single cell suspension preparation device of the utility model.

[0021] Figure 2 It is a side view of the single cell suspension preparation device of the utility model.

[0022] Figure 3 It is a top view of the single cell suspension preparation device of the utility model.

[0023] Figure 4 It is a partial structural schematic diagram of the single cell suspension preparation device of the utility model.

[0024] Figure 5 for Figure 4 Cross-sectional view at AA in the middle.

[0025] Figure 6 for Figure 5 Enlarged view of point B in the middle.

[0026] Figure 7 It is a cross-sectional view of the dissociation device of the utility model.

[0027] Figure 8 It is a structural schematic diagram of the heating device of the utility model.

[0028] Among them: 1. Shell; 2. Dissociation device; 201. Dissociation tube; 202. Rotating axis; 203. Dissociation seat; 3. Heating device; 301. Heating shell; 302. Mounting hole; 303. Positioning slot; 304. Heating tube; 4. Dustproof mechanism; 401. Dust cover; 402. Handle; 403. Buffer column; 5. Damping hinge; 6. Motor assembly; 601. Bracket; 602. Positioning block; 603. Motor; 604. Shock pad; 605. Coupling; 7. Power supply; 8. Base; 9. Control panel; 10. Display screen. DETAILED DESCRIPTION

[0029] Figures 1 to 8 It is the best embodiment of the utility model, and the following Figures 1 to 8 The utility model is further described.

[0030] like Figures 1 to 3As shown, a single cell suspension preparation device of the utility model includes a shell 1 and a base 8. The shell 1 is covered on the base 8 and fixed to the base 8 by bolts. The shell 1 can be disassembled from the base 8 during maintenance, which is convenient for maintenance. A through hole is opened on the top of the shell 1, and the bracket 601 of the motor 603 is placed in the through hole, and the bottom is fixed to the base 8. At least one rotatable motor 603 is arranged on the bracket 601, and the dissociation device 2 is installed above the motor 603 and connected to the output end of the motor 603. The heating device 3 is set outside the dissociation device 2 and clamped on the motor 603 component 6. A power supply 7 is set on the base 8, and the power supply 7 supplies power to the device.

[0031] Furthermore, a dust cover 401 is hingedly provided on the housing 1. When the device is not in use, the dust cover 401 is closed to prevent dust from entering the interior of the housing 1. A handle 402 is provided on the outside of the dust cover 401. The handle 402 is set as a hidden handle 402, which does not increase the volume of the device. A buffer column 403 is provided in the dust cover 401. One end of the buffer column 403 is fixed in the dust cover 401, and the other end is in contact with the top of the housing 1. The setting of the buffer column 403 prevents the dust cover 401 from colliding with the housing 1 when closed, thereby avoiding damage to the housing 1. In this embodiment, the dust cover 401 is hinged to the housing 1 through a damping hinge 5, so that the dust cover 401 can be opened at any angle for easy use. In this embodiment, the heating device 3, the dissociation device 2 and the motor 603 are all controlled by a control system. The control system includes a control panel 9 and a display screen 10. The control panel 9 is mounted on the base 8, and the display screen 10 is embedded in the front side of the housing 1.

[0032] Combination Figures 4 to 6 Specifically, the outer edge of the upper side of the bracket 601 is arranged close to the upper surface of the housing 1, and the bottom of the bracket 601 is fixed to the base 8 by bolts. In this embodiment, four groups of motors 603 are arranged on the bracket 601, and the motors 603 are respectively fixed to the bracket 601 by four bolts. The output end of the motor 603 is arranged through the top of the bracket 601, and the dissociation device 2 is connected to the output end of the motor 603 through the coupling 605. A shock-absorbing pad 604 is also arranged between the motor 603 and the bracket 601 to reduce vibration and noise.

[0033] See also Figure 7Further, the dissociation device 2 includes a dissociation seat 203, a dissociation tube 201 and a rotating shaft 202. The dissociation seat 203 is sleeved outside the rotating shaft 202, and a plug-in is provided at the bottom. The dissociation seat 203 is inserted into the bracket 601 through the plug-in and fixedly arranged with the bracket 601. One end of the rotating shaft 202 is connected to the output end of the motor 603, and at least one set of rotating cutter heads is installed at the other end, and the rotating cutter heads are in contact with the outer surface of the dissociation seat 203. In this embodiment, there is a protrusion with a special structure on the dissociation seat 203. When the rotating shaft 202 rotates, it drives the rotating cutter head to rub against the protrusion on the dissociation seat 203, thereby playing a role in dissociating and grinding cell tissues. The dissociation tube 201 is a transparent plastic tube, and the upper end has a thread that can be tightened and sealed with the dissociation seat 203, and the detachable cover of the dissociation tube 201 is arranged on the dissociation seat 203.

[0034] See also Figure 8 , at least one positioning block 602 is also provided on the top of the bracket 601, and the heating device 3 is docked with the positioning block 602. The heating device 3 is integrally formed and sleeved outside the dissociation device 2. The heating device 3 includes a heating shell 301, and mounting holes 302 are evenly provided on the heating shell 301. In this embodiment, the motor 603 and the dissociation tube 201 are each provided with four, and four mounting holes 302 are also provided. The heating shell 301 is covered outside the dissociation tube 201. A positioning groove 303 is provided on the outer ring of the heating device 3. The heating device 3 is clamped on the positioning block 602 at the top of the bracket 601 through the positioning groove 303. A number of heating tubes 304 are also evenly provided in the heating shell 301. The power supply 7 is connected through a quick-connect plug to achieve uniform heating of the dissociation tube 201. At the same time, the device is also equipped with a temperature control sensor, and the temperature is maintained at 37°C through the control system to ensure the constant temperature of the dissociation process.

[0035] When the utility model is used, the dust cover 401 is first opened, and then the power supply 7 is turned on. After the power supply 7 is turned on, the temperature of the heating tube 304 of the heating device 3 is controlled to be constant at 37°C through the display screen 10. The sample is loaded into the dissociation tube 201, and then the dissociation tube 201 containing the sample is installed on the dissociation seat 203. The rotating shaft 202 is driven to rotate by controlling the forward and reverse rotation of the motor 603, and the rotating cutter head is used to grind the sample, thereby obtaining a single cell suspension. When grinding, the number of samples to be ground can be selected according to demand.

[0036] The single cell suspension preparation device of the utility model has a shell 1 and a base 8 that can be detached and set, which is easy to disassemble and convenient to install and maintain; the dust cover 401 can prevent dust from entering when not in use, and the handle 402 is hidden and does not occupy extra volume; the heating device 3, the dissociation device 2 and the motor 603 can be accurately controlled by the display screen 10, the rotating shaft 202 of the dissociation device 2 is connected to the output end of the motor 603, driving the rotating cutter head to rub against the protrusion on the dissociation seat 203 to achieve dissociation and grinding, and the dissociation tube 201 is detachable and transparent; the heating shell 301 of the heating device 3 is integrally formed and sleeved outside the dissociation device 2, and the integrally formed design is convenient for disassembly and installation, the heating tube 304 can heat evenly, and the temperature control sensor ensures that the temperature is constant at 37°C. The utility model can automatically prepare multiple groups of single cell suspensions while keeping the temperature constant by installing multiple groups of dissociation devices 2 on the motor 603 that can be controlled separately, with small size, simple structure, convenient disassembly, short time and high efficiency.

[0037] The above is only the preferred embodiment of the utility model, and does not limit the utility model in other forms. Any technician familiar with the profession may use the technical content disclosed above to change or modify it into an equivalent embodiment with equivalent changes. However, any simple modification, equivalent change and modification made to the above embodiment based on the technical essence of the utility model without departing from the technical solution of the utility model still belongs to the protection scope of the technical solution of the utility model.

Claims

1. A single cell suspension preparation device, characterized in that: The invention comprises a housing (1), a base (8), a motor assembly (6), a dissociation device (2) and a heating device (3), wherein the motor assembly (6) is fixed to the base (8), and the housing (1) is disposed on the base (8); the motor assembly (6) comprises at least one rotatable motor (603); the dissociation device (2) is mounted above the motor (603) and connected to the output end of the motor (603); the heating device (3) is sleeved outside the dissociation device (2) and clamped on the motor assembly (6); a control system is also disposed on one side of the housing (1); and the motor assembly (6) and the heating device (3) are both electrically connected to the control system.

2. A single cell suspension preparation device according to claim 1, characterized in that: The motor assembly (6) comprises a bracket (601), a motor (603) and a coupling (605); the bottom of the bracket (601) is fixed to the base (8); the motor (603) is arranged on the bracket (601) in a forward and reverse manner; the output end of the motor (603) is arranged to pass through the top of the bracket (601); the dissociation device (2) is connected to the output end of the motor (603) via the coupling (605); at least one positioning block (602) is also arranged on the top of the bracket (601); and the heating device (3) is connected to the positioning block (602) to achieve docking.

3. A single cell suspension preparation device according to claim 1 or 2, characterized in that: The motors (603) are provided in 2 to 4 numbers, and each of the motors (603) is electrically connected to a control system.

4. The single cell suspension preparation device according to claim 1, characterized in that: The dissociation device (2) comprises a dissociation seat (203), a dissociation tube (201) and a rotating assembly; the dissociation seat (203) is sleeved outside the rotating assembly and fixedly arranged at the bottom with the motor assembly (6); one end of the rotating assembly is connected to the motor assembly (6) and the other end is in contact with the outer surface of the dissociation seat (203); and a detachable cover of the dissociation tube (201) is arranged on the dissociation seat (203).

5. A single cell suspension preparation device according to claim 4, characterized in that: The rotating assembly comprises a rotating shaft (202), one end of the rotating shaft (202) is connected to the motor assembly (6), and the other end is equipped with at least one set of rotating cutter heads, the rotating cutter heads are in contact with the outer edge surface of the dissociation seat (203).

6. The single cell suspension preparation device according to claim 1, characterized in that: The heating device (3) comprises a heating shell (301), mounting holes (302) are evenly arranged on the heating shell (301), the number of the mounting holes (302) is adapted to the number of the motor (603), the dissociation device (2) is mounted above the motor (603), and the heating shell (301) is covered outside the dissociation device (2); a positioning groove (303) is arranged on the outer ring of the heating device (3), and the heating device (3) is clamped on the motor assembly (6) through the positioning groove (303); and a plurality of heating tubes (304) are evenly arranged inside the heating shell (301).

7. The single cell suspension preparation device according to claim 1, characterized in that: The control system comprises a control panel (9) and a display screen (10); the control panel (9) is mounted on a base (8), and the display screen (10) is embedded on the front side of the housing (1).

8. The single cell suspension preparation device according to claim 1, characterized in that: It also includes a dustproof mechanism (4), which is hinged on the housing (1).

9. The single cell suspension preparation device according to claim 8, characterized in that: The dustproof mechanism (4) comprises a dustproof cover (401), the dustproof cover (401) being hinged on the outer shell (1), a handle (402) being provided on the outside of the dustproof cover (401), a buffer column (403) being provided inside the dustproof cover (401), one end of the buffer column (403) being fixed inside the dustproof cover (401), and the other end being in contact with the top of the outer shell (1).

10. The single cell suspension preparation device according to claim 9, characterized in that: The dust cover (401) is hinged to the outer shell (1) via a damping hinge (5).

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