Protein membrane transfer device for western blot

Abstract

The utility model discloses a protein membrane transfer device for western blot, which belongs to the technical field of western blot and comprises a containing box, a limiting component is slidably clamped in a cavity of the containing box, a bubble removing component is arranged at the top of the containing box and comprises a sliding rod, a limiting block acting on the sliding rod is arranged on one side of the top of the containing box, and the limiting block is connected with the sliding rod. Two connecting rods are fixed to the bottom of the sliding rod, rollers are rotationally connected to the bottoms of the opposite sides of the two connecting rods, the side wall of the sliding rod is rotationally sleeved with a traction sleeve, connecting plates for controlling the connecting rods to rotate are fixed to the tops of the opposite sides of the two connecting rods, and a traction rod facilitating force application is arranged on the side wall of the traction sleeve. When the protein transfer membrane device for western blot is used, the roller can be always in a state parallel to a laid material to discharge bubbles, so that the probability that the bubbles in part of positions cannot be discharged due to non-uniform stress of the material caused by inclination of the roller is reduced.

Description

Technical Field

[0001] The utility model belongs to the technical field of protein immunoblotting, and in particular relates to a protein membrane transfer device for protein immunoblotting. Background Art

[0002] In life science research, protein transfer apparatus is a key laboratory equipment used to transfer proteins separated by electrophoresis from gels to fixed support carriers, such as transferring proteins to PVDF membranes.

[0003] In Western Blot experiments, a transfer splint is required to transfer proteins to membranes. Materials such as sponges, filter paper, gels, and PVDF membranes are stacked between the two electrodes of the transfer splint. During the stacking process, it is necessary to ensure that there are no bubbles between each layer to ensure that the proteins in the gel can be completely transferred to the PVDF membrane. Therefore, laboratories generally use glass rods to drive out bubbles, and the operation is all done manually.

[0004] However, during the operation, it cannot be guaranteed that the glass rod is parallel to the materials to be stacked. If the glass rod is tilted relative to the sponge, filter paper, gel or PVDF membrane, the force may be too weak in some positions when the bubbles are driven out. Utility Model Content

[0005] The utility model aims to provide a protein transfer device for protein immunoblotting, so as to solve the problem that when a glass rod is used to drive out bubbles in the prior art mentioned in the above background technology, bubbles in some positions cannot be discharged because the glass rod is tilted relative to the sponge, filter paper, gel or PVDF membrane.

[0006] To achieve the above object, the utility model provides the following technical solutions: a protein transfer device for protein immunoblotting, comprising a containing box, a limiting component is slidably engaged in the cavity of the containing box, and a bubble-repelling component is arranged on the top;

[0007] The bubble-eliminating assembly comprises a slide bar, a limit block acting on the slide bar is arranged on one side of the top of the containing box, two connecting rods are fixed at the bottom of the slide bar, and rollers are rotatably connected at the bottom of the two connecting rods facing each other;

[0008] The sliding rod side wall rotating sleeve is provided with a traction sleeve.

[0009] As a preferred embodiment of the present utility model, a connecting plate for controlling the rotation of the connecting rods is fixed to the top of the two connecting rods on the opposite side.

[0010] As a preferred embodiment of the present utility model, the side wall of the traction sleeve is provided with a traction rod for facilitating force application.

[0011] As a preferred embodiment of the present invention, a groove for accommodating the slide rod is provided on the top of the containing box below the limiting block.

[0012] As a preferred embodiment of the present invention, limit plates are provided on the two opposite side walls of the containing box cavity, and the limit assembly includes a clamping plate, which is slidably clamped on the two limit plates.

[0013] As a preferred embodiment of the utility model, a pressing block is fixed at the bottom of the above-mentioned clamping plate, and a notch matched with the connecting rod is opened at the top of the above-mentioned clamping plate.

[0014] As a preferred embodiment of the present invention, a clamping block is provided on the side wall of the limit plate fixed in the containing box cavity, and the clamping block is used to support the clamping plate.

[0015] Beneficial Effects

[0016] Technical effects and advantages of this protein transfer device for protein immunoblotting:

[0017] When the transfer splint needs to be assembled, the transfer splint body is unfolded and placed in the cavity. When laying materials such as sponges, filter paper, gels, PVDF membranes, etc., each time a layer of material is laid, a roller parallel to the bottom of the containing box is used to roll once on the top of the laid material to discharge bubbles generated during laying. When the protein transfer device for protein immunoblotting is in use, the roller can be kept parallel to the laid material to discharge bubbles, thereby reducing the probability that bubbles in some positions cannot be discharged due to uneven force on the material caused by the tilt of the roller.

[0018] When using a roller to remove bubbles, the forces on different positions of the laid material can be kept in a relatively similar state, and the forces will not be too large, thereby reducing the probability of gel rupture due to excessive force on the laid material.

[0019] After the bubbles generated when one layer of material is laid are discharged, the slide rod is slid to the groove on the top of the containing box to temporarily limit the position of the bubble-removing component. This structure allows the bubble-removing component to be immersed in the buffer solution in the cavity when not discharging bubbles, thereby reducing the possibility of the roller being contaminated by other substances. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the specific implementation methods of the utility model or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0021] Figure 1This is a schematic diagram of the structure of the protein transfer device used for protein immunoblotting;

[0022] Figure 2 for Figure 1 Partial structural decomposition diagram;

[0023] Figure 3 for Figure 2 A schematic diagram of the structure of the middle limit assembly;

[0024] Figure 4 for Figure 2 Schematic diagram of the structure of the middle bubble-chasing component;

[0025] Figure 5 A schematic diagram of the structure for loosening the limit assembly and temporarily limiting the position of the bubble-eliminating assembly.

[0026] In the figure:

[0027] 11. Container; 12. Limiting plate; 13. Block; 14. Limiting block; 15. Groove;

[0028] 2. Transfer splint body;

[0029] 3. Limiting assembly; 31. Card plate; 32. Pressing block; 33. Notch;

[0030] 4. Bubble-removing assembly; 41. Sliding rod; 42. Connecting rod; 421. Connecting plate; 43. Roller; 44. Traction sleeve; 441. Traction rod. DETAILED DESCRIPTION

[0031] In the following description, a large number of specific details are given to provide a more thorough understanding of the present invention. However, it is obvious to those skilled in the art that the present invention can be implemented without one or more of these details. In order to avoid confusion with the present invention, some technical features known in the art are not described.

[0032] Unless otherwise defined, the directions of up, down, left, right, front, back, inside and outside involved in this document are based on the directions of up, down, left, right, front, back, inside and outside in the figures shown in the present utility model, and are explained here together.

[0033] The connection method can adopt existing methods such as bonding, welding, bolt connection, etc., depending on actual needs.

[0034] Please refer to Figure 1-Figure 5 The protein transfer device for protein immunoblotting shown includes a containing box 11. When it is necessary to transfer the protein separated by electrophoresis from the gel to a fixed support carrier, the buffer solution is first introduced into the containing box 11, and the transfer splint body 2 is selected and placed in the containing box 11.

[0035] The material herein refers to any one between sponge, filter paper, gel and PVDF membrane.

[0036] In this embodiment, a bubble-eliminating assembly 4 is provided on the top of the containing box 11, and the bubble-eliminating assembly 4 includes a slide bar 41. A limit block 14 acting on the slide bar 41 is provided on one side of the top of the containing box 11. Two connecting rods 42 are fixed to the bottom of the slide bar 41, and the bottoms of the two connecting rods 42 facing each other are rotatably connected with rollers 43.

[0037] like Figure 1 , Figure 4 As shown, the bubble-eliminating component 4 is constrained on the containing box 11 by the limiting block 14, and each time a layer of material is laid, the roller 43 is dragged once to discharge bubbles.

[0038] In this embodiment, a groove 15 for accommodating the slide rod 41 is provided at the top of the containing box 11 below the limiting block 14 .

[0039] like Figure 5 As shown, when laying materials, the slide rod 41 of the bubble-eliminating component 4 can be slid and placed in the groove 15. At this time, the roller 43 is in the buffer solution, which can reduce the probability of the roller 43 contacting other impurities and causing it to be contaminated.

[0040] In this embodiment, a traction sleeve 44 is rotatably provided on the side wall of the slide rod 41 , and a traction rod 441 is provided on the side wall of the traction sleeve 44 for applying force.

[0041] like Figure 4 As shown, when it is necessary to move the roller 43 to discharge bubbles, force can be applied to the traction rod 441. Since the traction sleeve 44 and the slide rod 41 are rotatably connected, and the slide rod 41 is constrained by the limit block 14, when force acts on the traction rod 441, the direction of the force will not affect the slide rod 41 in the vertical direction. The direction of the force is only used to change the direction of movement of the slide rod 41, so that the force applied to the traction rod 441 will not affect the roller 43 in the vertical direction, thereby ensuring that the roller 43 acts on the material only under its own gravity, reducing the possibility of a sudden increase in the force acting on the gel.

[0042] In this embodiment, a connecting plate 421 for controlling the rotation of the connecting rods 42 is fixed to the top of the two connecting rods 42 on the opposite side.

[0043] like Figure 4 As shown, when laying materials during use, the slide bar 41 of the bubble-eliminating component 4 can be temporarily placed in the groove 15 to temporarily restrict its movement. After laying part of the material, the height of the top of the material will be higher than the height of the roller 43. At this time, it is necessary to drive the roller 43 to rotate and stick to the top of the material through the connecting plate 421, and then drive the roller 43 to move and discharge bubbles through the traction rod 441.

[0044] In this embodiment, the limiting component 3 is slidably clamped in the cavity, and limiting plates 12 are arranged on the opposite side walls of the cavity of the containing box 11. The limiting component 3 includes a clamping plate 31, and the clamping plate 31 is slidably clamped on the two limiting plates 12. A pressing block 32 is fixed at the bottom of the clamping plate 31, and a groove 33 matching the connecting rod 42 is opened at the top of the clamping plate 31. Clamping blocks 13 are arranged on the side walls of the limiting plates 12 fixed in the cavity of the containing box 11, and the clamping blocks 13 are used to support the clamping plate 31.

[0045] like Figure 1-Figure 3 As shown, when the material is stacked on the transfer splint body 2, the limiting assembly 3 is in the limiting plate 12. At this time, the pressing block 32 will press against the top of the transfer splint body 2 and constrain the position of the transfer splint body 2. When laying the material, one side of the material is close to the card board 31. During the bubble discharge process, when the roller 43 is dragged close to the limiting assembly 3, the roller 43 will press against the card board 31 to prevent it from falling from the top of the material. At this time, the connecting rod 42 is located in the slot 33, so that the roller 43 can be attached to the card board 31.

[0046] Working principle: when the transfer splint needs to be assembled, unfold the transfer splint body 2 and place it in the cavity of the containing box 11. When laying materials such as sponge, filter paper, gel, PVDF membrane, etc., each time a layer of material is laid, the connecting rod 42 and the roller 43 are rotated and lifted through the connecting plate 421, and then the roller 43 is placed on the laid material. Then, the sliding rod 41, the connecting rod 42 and the roller 43 are pulled and rotated through the traction rod 441, so that the roller 43 can roll on the top of the laid material to discharge the bubbles generated during laying.

[0047] After the bubbles generated when one layer of material is laid are exhausted, the slide bar 41 is slid to the groove 15 at the top of the containing box 11 to temporarily limit the position of the bubble-eliminating component 4 .

[0048] Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A protein transfer device for protein immunoblotting, comprising a containing box (11), characterized in that: The containing box (11) is slidably engaged with a limiting component (3) in the cavity, and a bubble-removing component (4) is arranged on the top; The bubble-eliminating component (4) comprises a slide bar (41), a limit block (14) acting on the slide bar (41) is arranged on one side of the top of the containing box (11), two connecting rods (42) are fixed on the bottom of the slide bar (41), and rollers (43) are rotatably connected at the bottoms of the two connecting rods (42) facing each other; The side wall of the slide bar (41) is rotatably sleeved with a traction sleeve (44).

2. The protein transfer device for protein immunoblotting according to claim 1, characterized in that: A connecting plate (421) for controlling the rotation of the connecting rods (42) is fixed to the tops of the two connecting rods (42) on the opposite sides.

3. The protein transfer device for protein immunoblotting according to claim 1, characterized in that: The side wall of the traction sleeve (44) is provided with a traction rod (441) for facilitating force application.

4. The protein transfer device for protein immunoblotting according to claim 1, characterized in that: A groove (15) for accommodating the slide rod (41) is provided at the top of the containing box (11) below the limiting block (14).

5. The protein transfer device for protein immunoblotting according to claim 1, characterized in that: Limiting plates (12) are arranged on opposite side walls of the cavity of the containing box (11), and the limiting assembly (3) comprises a clamping plate (31), and the clamping plate (31) is slidably clamped on the two limiting plates (12).

6. The protein transfer device for protein immunoblotting according to claim 5, characterized in that: A pressing block (32) is fixed at the bottom of the clamping plate (31), and a notch (33) matching with the connecting rod (42) is formed at the top of the clamping plate (31).

7. The protein transfer device for protein immunoblotting according to claim 6, characterized in that: A clamping block (13) is provided on the side wall of the limit plate (12) fixed in the cavity of the containing box (11), and the clamping block (13) is used to support the clamping plate (31).

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