Ultrafiltration centrifuge tube for secondary separation

Abstract

The ultrafiltration centrifugal tube comprises an outer tube, a tube cap connected to the opening end of the outer tube and an inner tube assembly coaxially arranged in the outer tube, the inner tube assembly comprises a first inner tube and a second inner tube coaxially and detachably installed at the bottom end of the first inner tube, a first ultrafiltration membrane is obliquely installed on the inner wall of the first inner tube, and a second ultrafiltration membrane is obliquely installed on the inner wall of the second inner tube. A second ultrafiltration membrane is obliquely mounted on the inner wall of the second inner pipe, the inclination angles and the inclination directions of the first ultrafiltration membrane and the second ultrafiltration membrane are the same, and the cutting molecular weight of the first ultrafiltration membrane is larger than that of the second ultrafiltration membrane. According to the ultrafiltration centrifugal tube, the first ultrafiltration membrane and the second ultrafiltration membrane are arranged, and the cut molecular weight of the first ultrafiltration membrane is greater than that of the second ultrafiltration membrane, so that secondary separation is realized by primary centrifugation of the ultrafiltration centrifugal tube, the operation steps of centrifugation are reduced, and the operation time of centrifugation is shortened.

Description

Technical Field

[0001] The utility model relates to the technical field of ultrafiltration centrifuge tubes, and more specifically to an ultrafiltration centrifuge tube for secondary separation. Background Art

[0002] Centrifugal technology has been widely used in biological sciences, especially in the fields of biochemistry and molecular biology research. Centrifuge tubes are important equipment in centrifugal technology, generally tubular containers with lids. Ultrafiltration centrifuge tubes are centrifuge tubes with ultrafiltration membranes. The ultrafiltration membranes can trap particles and achieve rapid separation of particles from solutions.

[0003] Existing ultrafiltration centrifuge tubes are generally ultrafiltration centrifuge tubes with a single ultrafiltration membrane. One centrifugation can only separate the sample once. If multiple separations are required, multiple centrifugations are required, which increases the number of centrifugation operation steps and operation time.

[0004] Therefore, it is necessary to propose a new solution to solve the above problems. Utility Model Content

[0005] The utility model aims to overcome the above-mentioned deficiencies of the prior art and provide an ultrafiltration centrifuge tube for secondary separation.

[0006] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0007] A secondary separation ultrafiltration centrifugal tube comprises an outer tube, a tube cap connected to the open end of the outer tube, and an inner tube assembly coaxially arranged in the outer tube, the inner tube assembly comprises a first inner tube, and a second inner tube coaxially and detachably installed on the bottom end of the first inner tube, a first ultrafiltration membrane is obliquely installed on the inner wall of the first inner tube, a second ultrafiltration membrane is obliquely installed on the inner wall of the second inner tube, the first ultrafiltration membrane and the second ultrafiltration membrane have the same inclination angle and inclination direction, and the cut-off molecular weight of the first ultrafiltration membrane is greater than the cut-off molecular weight of the second ultrafiltration membrane.

[0008] Furthermore, a first inclined plate for installing a first ultrafiltration membrane is obliquely installed on the inner wall of the first inner tube, and a second inclined plate for installing a second ultrafiltration membrane is obliquely installed on the inner wall of the second inner tube, and the first inclined plate and the second inclined plate have the same inclination angle and inclination direction.

[0009] Furthermore, a first mounting groove is provided on the upper surface of the first inclined plate, a first supporting rib is installed in the middle of the first mounting groove, a first through hole is provided in the lower part of the first mounting groove, the first ultrafiltration membrane is installed in the first mounting groove, the first ultrafiltration membrane abuts against the first supporting rib and covers the first through hole.

[0010] Furthermore, a second mounting groove is provided on the upper surface of the second inclined plate, a second supporting rib is installed in the middle of the second mounting groove, a second through hole is provided in the lower part of the second mounting groove, the second ultrafiltration membrane is installed in the second mounting groove, the second ultrafiltration membrane abuts the second supporting rib and covers the second through hole.

[0011] Furthermore, a rubber ring is provided at the bottom end of the first inner tube, an outer wall of the rubber ring is provided with an external thread, an inner wall of the top end of the second inner tube is provided with an internal thread, and the first inner tube is connected to the second inner tube through a threaded connection of the external thread and the internal thread.

[0012] Furthermore, two radially symmetrical support blocks are provided on the inner wall of the lower part of the outer tube, and the top end of the first inner tube is provided with a convex edge that can be placed on the open end of the outer tube. When the inner tube assembly is placed in the outer tube, the convex edge of the first inner tube abuts the open end of the outer tube, and the bottom end of the second inner tube abuts the support block.

[0013] Furthermore, the tube cap is threadedly connected to the open end of the outer tube, and the inner wall of the tube cap abuts against the first inner tube.

[0014] Furthermore, the cut-off molecular weight of the first ultrafiltration membrane is at least 5 times that of the cut-off molecular weight of the second ultrafiltration membrane.

[0015] The beneficial effects of the utility model are:

[0016] 1. In the utility model, by providing a first ultrafiltration membrane and a second ultrafiltration membrane, and the cut-off molecular weight of the first ultrafiltration membrane is greater than the cut-off molecular weight of the second ultrafiltration membrane, the ultrafiltration centrifuge tube can achieve secondary separation by one centrifugation, thereby reducing the operation steps of centrifugation and shortening the operation time of centrifugation.

[0017] 2. In the utility model, the first ultrafiltration membrane and the second ultrafiltration membrane are tilted so that when the sample is centrifuged, the sample flows tangentially on the surfaces of the first ultrafiltration membrane and the second ultrafiltration membrane, thereby improving the filtration effect; at the same time, the first ultrafiltration membrane and the second ultrafiltration membrane are tilted at the same angle and in the same direction to avoid turbulence when the sample is centrifuged, thereby avoiding damage to the sample cells.

[0018] 3. In the utility model, a rubber ring and a threaded connection between an external thread and an internal thread are provided to achieve a sealed connection between the first inner tube and the second inner tube, so that when the sample is centrifuged, the sample can only flow from the second inner tube through the first through hole and the first ultrafiltration membrane to the first inner tube, and from the first inner tube through the first ultrafiltration membrane and the first through hole to the second inner tube.

[0019] 4. In the utility model, a support pad is provided on the inner wall of the lower part of the outer tube, and a convex edge is provided on the top end of the first inner tube. When the tube cap is threadedly connected to the open end of the outer tube, the tube cap abuts against the convex edge of the first inner tube, and the support pad abuts against the bottom end of the second inner tube, so as to limit the first inner tube and the second inner tube, thereby preventing the first inner tube and the second inner tube from shaking in the outer tube when the sample is centrifuged, thereby affecting the centrifugal effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 A schematic diagram of the structure of the ultrafiltration centrifuge tube in this embodiment;

[0021] Figure 2 A schematic cross-sectional view of the ultrafiltration centrifuge tube in this embodiment;

[0022] Figure 3 is a structural schematic diagram of the first inner tube in this embodiment;

[0023] Figure 4 This is a schematic diagram of a connection structure between the first inclined plate and the first inner tube in this embodiment;

[0024] Figure 5 is a structural schematic diagram of the first inclined plate in this embodiment;

[0025] Figure 6 is a structural schematic diagram of the second inner tube in this embodiment;

[0026] Figure 7 Schematic diagram of the structure of the second inclined plate in this embodiment.

[0027] Figure numerals: outer tube 1, support pad 101, tube cap 2, inner tube assembly 3, first inner tube 301, first ultrafiltration membrane 302, first inclined plate 303, first mounting groove 304, first support rib 305, first through hole 306, second inner tube 307, second ultrafiltration membrane 308, second inclined plate 309, second mounting groove 310, second support rib 311, second through hole 312, rubber ring 313, external thread 314, internal thread 315, flange 316. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0029] Embodiment: A secondary separation ultrafiltration centrifuge tube, such as Figure 1-Figure 7As shown, it includes an outer tube 1, a tube cap 2 connected to the open end of the outer tube 1, and an inner tube assembly 3 coaxially arranged in the outer tube 1, wherein the inner tube assembly 3 includes a first inner tube 301, and a second inner tube 307 coaxially and detachably installed on the bottom end of the first inner tube 301, the first inner tube 301 and the second inner tube 307 are made of plastic material, the inner wall of the first inner tube 301 is obliquely installed with a first ultrafiltration membrane 302, the inner wall of the second inner tube 307 is obliquely installed with a second ultrafiltration membrane 308, the first ultrafiltration membrane 302 and the second ultrafiltration membrane 308 have the same inclination angle and inclination direction, and the cut-off molecular weight of the first ultrafiltration membrane 302 is greater than the cut-off molecular weight of the second ultrafiltration membrane 308.

[0030] By setting the first ultrafiltration membrane 302 and the second ultrafiltration membrane 308, and the cut-off molecular weight of the first ultrafiltration membrane 302 is greater than the cut-off molecular weight of the second ultrafiltration membrane 308, the ultrafiltration centrifuge tube can achieve secondary separation in one centrifugation, reduce the operation steps of centrifugation, and shorten the operation time of centrifugation. If the cut-off molecular weight of the first ultrafiltration membrane 302 is set to at least 5 times the cut-off molecular weight of the second ultrafiltration membrane 308, the mixed protein can be separated twice in one centrifugation.

[0031] By tilting the first ultrafiltration membrane 302 and the second ultrafiltration membrane 308, when the sample is centrifuged, the sample flows tangentially on the surfaces of the first ultrafiltration membrane 302 and the second ultrafiltration membrane 308, thereby improving the filtration effect; at the same time, the first ultrafiltration membrane 302 and the second ultrafiltration membrane 308 are tilted at the same angle and in the same direction to avoid turbulence when the sample is centrifuged, thereby preventing the sample cells from being destroyed.

[0032] like Figure 2 As shown, the inner wall of the first inner tube 301 is obliquely installed with a first inclined plate 303 for installing the first ultrafiltration membrane 302, and the inner wall of the second inner tube 307 is obliquely installed with a second inclined plate 309 for installing the second ultrafiltration membrane 308, and the first inclined plate 303 and the second inclined plate 309 have the same inclination angle and inclination direction. The first inclined plate 303 and the second inclined plate 309 are made of plastic material; the first inclined plate 303 and the second inclined plate 309 are provided to facilitate the installation of the first ultrafiltration membrane 302 and the second ultrafiltration membrane 308; at the same time, the edge of the first inclined plate 303 is connected to the inner wall of the first inner tube 301 to achieve a sealed connection between the first inclined plate 303 and the first inner tube 301, and the edge of the second inclined plate 309 is connected to the inner wall of the second inner tube 307 to achieve a sealed connection between the second inclined plate 309 and the second inner tube 307.

[0033] Further, such as Figure 4 , Figure 5As shown, the upper surface of the first inclined plate 303 is provided with a first mounting groove 304, the middle part of the first mounting groove 304 is provided with a first support rib 305, the lower part of the first mounting groove 304 is provided with a first through hole 306, the first ultrafiltration membrane 302 is installed in the first mounting groove 304, the first ultrafiltration membrane 302 abuts against the first support rib 305, and covers the first through hole 306. By providing the first support rib 305, the first ultrafiltration membrane 302 can be supported to prevent the first ultrafiltration membrane 302 from deforming under the centrifugal impact of the sample. By providing the first through hole 306, the sample can flow from the second inner tube 307 through the first through hole 306 and the first ultrafiltration membrane 302 to the first inner tube 301, and from the first inner tube 301 through the first ultrafiltration membrane 302 and the first through hole 306 to the second inner tube 307.

[0034] like Figure 6 , Figure 7 As shown, the upper surface of the second inclined plate 309 is provided with a second mounting groove 310, the middle part of the second mounting groove 310 is provided with a second support rib 311, the lower part of the second mounting groove 310 is provided with a second through hole 312, the second ultrafiltration membrane 308 is installed in the second mounting groove 310, the second ultrafiltration membrane 308 abuts the second support rib 311, and covers the second through hole 312. By providing the second support rib 311, the second ultrafiltration membrane 308 can be supported to prevent the second ultrafiltration membrane 308 from deforming under the centrifugal impact of the sample. By providing the second through hole 312, the sample can flow from the outer tube 1 through the second through hole 312 and the second ultrafiltration membrane 308 to the second inner tube 307, and from the second inner tube 307 through the second ultrafiltration membrane 308 and the second through hole 312 to the outer tube 1.

[0035] Further, such as Figure 3 , Figure 6 , Figure 7 As shown, a rubber ring 313 is provided at the bottom end of the first inner tube 301, and an external thread 314 is provided on the outer wall of the rubber ring 313. An internal thread 315 matching the external thread 314 is provided on the inner wall of the top end of the second inner tube 307. The first inner tube 301 is connected to the second inner tube 307 through the threaded connection of the external thread 314 and the internal thread 315. The rubber ring 313 and the threaded connection of the external thread 314 and the internal thread 315 are provided to achieve a sealed connection between the first inner tube 301 and the second inner tube 307, so that when the sample is centrifuged, the sample can only flow from the second inner tube 307 to the first inner tube 301 through the first through hole 306 and the first ultrafiltration membrane 302, and flow from the first inner tube 301 to the second inner tube 307 through the first ultrafiltration membrane 302 and the first through hole 306.

[0036] like Figure 2 , Figure 3 , Figure 4 , Figure 5As shown, two support blocks 101 are provided on the inner wall of the lower part of the outer tube 1, and the two support blocks 101 are radially symmetrical. The top end of the first inner tube 301 is provided with a flange 316 that can be placed on the open end of the outer tube 1. When the inner tube assembly 3 is placed in the outer tube 1, the flange 316 of the first inner tube 301 abuts against the open end of the outer tube 1, and the bottom end of the second inner tube 307 abuts against the support block 101. The support block 101 and the flange 316 cooperate to limit the installation position of the inner tube assembly 3.

[0037] Further, such as Figure 2 As shown, the tube cap 2 is threadedly connected to the open end of the outer tube 1, and the inner wall of the tube cap 2 abuts the first inner tube 301. With the above design, on the one hand, the open end of the outer tube 1 can be sealed by the tube cap 2, and on the other hand, the tube cap 2 abuts against the convex edge 316 of the first inner tube 301, and the support pad 101 abuts against the bottom end of the second inner tube 307, so as to limit the first inner tube 301 and the second inner tube 307, so as to prevent the first inner tube 301 and the second inner tube 307 from shaking in the outer tube 1 when the sample is centrifuged, thereby affecting the centrifugal effect.

[0038] The above is only a preferred embodiment of the present invention. The protection scope of the present invention is not limited to the above embodiments. All technical solutions under the concept of the present invention belong to the protection scope of the present invention. It should be pointed out that for ordinary technicians in this technical field, some improvements and modifications without departing from the principle of the present invention should also be regarded as the protection scope of the present invention.

Claims

1. An ultrafiltration centrifuge tube for secondary separation, comprising an outer tube (1), a tube cap (2) connected to the open end of the outer tube (1), and an inner tube assembly (3) coaxially arranged in the outer tube (1), characterized in that: The inner tube assembly (3) comprises a first inner tube (301) and a second inner tube (307) coaxially and detachably mounted on the bottom end of the first inner tube (301); a first ultrafiltration membrane (302) is obliquely mounted on the inner wall of the first inner tube (301); a second ultrafiltration membrane (308) is obliquely mounted on the inner wall of the second inner tube (307); the first ultrafiltration membrane (302) and the second ultrafiltration membrane (308) have the same inclination angle and inclination direction, and the cut-off molecular weight of the first ultrafiltration membrane (302) is greater than the cut-off molecular weight of the second ultrafiltration membrane (308).

2. The secondary separation ultrafiltration centrifuge tube according to claim 1, characterized in that: A first inclined plate (303) for mounting a first ultrafiltration membrane (302) is obliquely installed on the inner wall of the first inner tube (301), and a second inclined plate (309) for mounting a second ultrafiltration membrane (308) is obliquely installed on the inner wall of the second inner tube (307), wherein the first inclined plate (303) and the second inclined plate (309) have the same inclination angle and inclination direction.

3. The secondary separation ultrafiltration centrifuge tube according to claim 2, characterized in that: A first mounting groove (304) is provided on the upper surface of the first inclined plate (303), a first supporting rib (305) is installed in the middle of the first mounting groove (304), a first through hole (306) is provided in the lower part of the first mounting groove (304), and the first ultrafiltration membrane (302) is installed in the first mounting groove (304), the first ultrafiltration membrane (302) abuts against the first supporting rib (305) and covers the first through hole (306).

4. The secondary separation ultrafiltration centrifuge tube according to claim 2, characterized in that: A second mounting groove (310) is provided on the upper surface of the second inclined plate (309), a second supporting rib (311) is installed in the middle of the second mounting groove (310), a second through hole (312) is provided in the lower part of the second mounting groove (310), and the second ultrafiltration membrane (308) is installed in the second mounting groove (310), the second ultrafiltration membrane (308) abuts against the second supporting rib (311) and covers the second through hole (312).

5. The secondary separation ultrafiltration centrifuge tube according to claim 1, characterized in that: A rubber ring (313) is provided at the bottom end of the first inner tube (301), an external thread (314) is provided on the outer wall of the rubber ring (313), an internal thread (315) is provided on the inner wall of the top end of the second inner tube (307), and the first inner tube (301) is connected to the second inner tube (307) through the threaded connection of the external thread (314) and the internal thread (315).

6. The secondary separation ultrafiltration centrifuge tube according to claim 1, characterized in that: Two radially symmetrical support blocks (101) are provided on the inner wall of the lower part of the outer tube (1); a convex edge (316) which can be placed on the open end of the outer tube (1) is provided at the top end of the first inner tube (301); when the inner tube assembly (3) is placed in the outer tube (1), the convex edge (316) of the first inner tube (301) abuts against the open end of the outer tube (1), and the bottom end of the second inner tube (307) abuts against the support block (101).

7. The secondary separation ultrafiltration centrifuge tube according to claim 1, characterized in that: The pipe cap (2) is threadedly connected to the open end of the outer pipe (1), and the inner wall of the pipe cap (2) abuts against the first inner pipe (301).

8. The secondary separation ultrafiltration centrifuge tube according to claim 1, characterized in that: The cut-off molecular weight of the first ultrafiltration membrane (302) is at least 5 times the cut-off molecular weight of the second ultrafiltration membrane (308).

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