Patch clamp dosing device

Abstract

The utility model provides a patch clamp dosing device which comprises an installation support, a medicine pushing cylinder, a medicine feeding head, a pneumatic push rod, a gas cylinder, a water tank and a flushing pipe, the medicine pushing cylinder is arranged on the installation support, a pushing rod is connected in the medicine pushing cylinder in a sliding mode, the pneumatic push rod is connected to the upper end of the pushing rod, and the gas cylinder is connected with the pneumatic push rod through a gas supply header pipe. A lower opening of the medicine pushing cylinder is connected with the medicine feeding head through a liquid feeding pipe, the flushing pipe is connected between the water tank and the liquid feeding pipe, a branch pipe is connected between the air supply header pipe and the water tank, and an airflow control valve is arranged on the branch pipe. According to the patch clamp dosing device provided by the utility model, the pushing rod of the medicine pushing cylinder is pushed and pressed down by virtue of the pneumatic push rod, so that liquid medicine in the medicine pushing cylinder enters the liquid feeding pipe, and when the liquid medicine needs to be replaced, the airflow control valve is opened to supply air into the water tank, so that water in the water tank flows into the flushing pipe and the liquid feeding pipe under the action of air pressure; the liquid feeding pipe is flushed, so that different liquid medicines can be conveniently replaced subsequently, the cross contamination is avoided, and the test accuracy is improved.

Description

Technical Field

[0001] The utility model belongs to the technical field of patch clamp structures, and more specifically relates to a patch clamp drug adding device. Background Art

[0002] As a device that can measure the cell membrane surface, patch clamp is widely used. Patch clamp technology reflects the activity of single or multiple ion channels on the cell membrane by recording the ion currents of various ion channels on the cell membrane. The application of patch clamp technology can conduct in-depth research on the existence of ion channels on the cell membrane, their electrophysiological characteristics, molecular structure, drug action mechanism, etc.

[0003] In the patch clamp experiment, the effect of the drug to be tested on the ion channel can be measured by local perfusion of the whole cell or the membrane. The existing patch clamp dosing device is prone to electromagnetic interference, which in turn has an adverse effect on the normal operation of the patch clamp. In addition, the above-mentioned dosing device also has the problem of inconvenient pipeline flushing, which easily causes cross contamination of different reagents, affecting the accuracy of the test data. Utility Model Content

[0004] The utility model aims to provide a patch clamp drug adding device, which can conveniently flush the pipeline, avoid cross contamination of different reagents, and avoid the influence of electromagnetic interference.

[0005] To achieve the above-mentioned purpose, the technical solution adopted by the utility model is: to provide a patch clamp dosing device, including a mounting bracket, a drug pushing cartridge, a drug delivery head, a pneumatic push rod, a gas cylinder, a water tank and a flushing pipe, the drug pushing cartridge is arranged on the mounting bracket along the up and down directions, a push rod is slidably connected in the drug pushing cartridge, the pneumatic push rod is connected to the upper end of the push rod and is used to push the push rod downward, the gas cylinder is connected to the pneumatic push rod through the gas supply main pipe, the lower end of the drug pushing cartridge is connected to the drug delivery head through the liquid delivery pipe, the flushing pipe is connected between the water tank and the liquid delivery pipe, and a control ball valve is provided at the lower part of the drug pushing cartridge;

[0006] Among them, a branch pipe is connected between the gas supply main pipe and the water tank, and an air flow control valve is arranged on the branch pipe. The water tank can supply flushing water to the liquid delivery pipe when the air flow control valve is opened.

[0007] In a possible implementation, a plurality of drug push cartridges and a plurality of pneumatic push rods are provided respectively, and the plurality of drug push cartridges and the plurality of pneumatic push rods are provided in one-to-one correspondence, and the plurality of pneumatic push rods are provided at intervals along the axial direction of the gas supply main pipe.

[0008] In some embodiments, the upper end of the pneumatic push rod is connected to the main air supply pipe through an air supply branch pipe, and a pneumatic throttle valve and a pneumatic solenoid valve are provided on the air supply branch pipe, and the pneumatic throttle valve is located above the pneumatic solenoid valve.

[0009] In some embodiments, the gas cylinder is connected to the middle of the gas supply main pipe through the gas delivery pipe, and the branch pipe is connected to the end of the gas supply main pipe.

[0010] In a possible implementation, the flushing pipe is connected to the inlet end of the liquid delivery pipe via a flushing branch, and a sub-control valve is provided on the flushing branch.

[0011] In a possible implementation, a buffer damper is provided between the medicine pushing cartridge and the pneumatic push rod, the upper end of the buffer damper is connected to the pushing end of the pneumatic push rod, and the lower end of the buffer damper is connected to the upper end of the push rod.

[0012] In a possible implementation, the patch clamp dosing device also includes an operating cabinet, the mounting bracket is connected to the inner wall of the operating cabinet, the drug pushing cartridge, the pneumatic push rod and the drug delivery head are respectively located in the operating cabinet, and the water tank and the gas cylinder are respectively arranged on the top of the operating cabinet.

[0013] In a possible implementation, the mounting bracket includes a frame body, a first transverse plate and a second transverse plate, the first transverse plate and the second transverse plate are respectively connected to the front side of the frame body, the first transverse plate is provided with a first slot that is clamped to the body of the drug pushing cartridge, the first transverse plate is used to support the bottom of the upper end plate of the drug pushing cartridge, the second transverse plate is located below the first transverse plate, the second transverse plate is provided with a second slot that is clamped to the body of the drug pushing cartridge, the second slot is arranged correspondingly to the first slot up and down, and the second slot is clamped to the lower periphery of the drug pushing cartridge.

[0014] In some embodiments, a limiting column extending in the up-down direction is provided on the frame, and two sides of the drug pushing cartridge are symmetrically provided with engaging claws capable of clamping on both sides of the limiting column, and the extending ends of the two engaging claws extend toward adjacent sides.

[0015] In a possible implementation, a liquid collecting seat connected to the liquid outlet pipe of the liquid delivery pipe and used to collect the liquid medicine in the liquid delivery pipe is provided above the drug delivery head, and a collecting cavity connected to the drug delivery head is provided in the liquid collecting seat.

[0016] Compared with the prior art, the scheme shown in the embodiment of the present application is a patch clamp dosing device provided in the embodiment of the present application, which uses a mounting bracket to install and fix the drug push cartridge, and uses a pneumatic push rod to push down the push rod of the drug push cartridge, so that the drug liquid in the drug push cartridge enters the liquid delivery tube, avoiding the problem of electromagnetic interference during the driving process. When the drug liquid needs to be replaced, the air flow control valve is opened to supply gas into the water tank, and then the water in the water tank flows into the flushing tube and the liquid delivery tube under the action of air pressure, thereby flushing the liquid delivery tube, facilitating the subsequent replacement of different drug liquids, avoiding cross contamination during the drug change process, improving the accuracy of the test, and improving the operating efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0018] Figure 1 A schematic diagram of the front and cross-sectional structure of a patch clamp dosing device provided in an embodiment of the utility model;

[0019] Figure 2 For the utility model embodiment Figure 1 The partially enlarged structural diagram of middle Ⅰ;

[0020] Figure 3 For the utility model embodiment Figure 1 Schematic diagram of the local structure of AA;

[0021] Figure 4 For the utility model embodiment Figure 1 The left side structural diagram of the mounting bracket and the cartridge pusher;

[0022] Figure 5 For the utility model embodiment Figure 4 Schematic diagram of the cross-sectional structure of the middle BB;

[0023] Figure 6 For the utility model embodiment Figure 1 Schematic diagram of the left side cross-sectional structure of the mounting bracket.

[0024] Among them, the reference numerals in the figure are:

[0025] 1. Mounting bracket; 11. Frame; 12. First horizontal plate; 13. Second horizontal plate; 14. First card slot; 15. Second card slot; 16. Limiting column; 2. Push cartridge; 21. Push rod; 22. Control ball valve; 23. Liquid delivery pipe; 24. Clamping claw; 3. Drug delivery head; 4. Pneumatic push rod; 41. Buffer damper; 5. Gas cylinder; 51. Gas supply main pipe; 52. Branch pipe; 53. Air flow control valve; 54. Gas supply branch pipe; 541. Pneumatic throttle valve; 542. Pneumatic solenoid valve; 543. Pneumatic regulating joint; 55. Air delivery pipe; 6. Water tank; 7. Flushing pipe; 71. Flushing branch; 72. Sub-control valve; 8. Operation cabinet; 9. Liquid collection seat. DETAILED DESCRIPTION

[0026] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0027] It should be noted that when an element is referred to as being "disposed on" another element, it may be directly on the other element or indirectly on the other element. It should be understood that the orientation or positional relationship indicated by the terms "length", "width", "upper", "lower", "front", "back", "top", "bottom", "inside", "outside", etc. is based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing the utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the utility model. The terms "first" and "second" are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the utility model, the meaning of "several" is two or more, unless otherwise clearly and specifically defined.

[0028] Please also read Figures 1 to 6 , the patch clamp dosing device provided by the utility model is now described. The patch clamp dosing device includes a mounting bracket 1, a drug pushing cartridge 2, a drug delivery head 3, a pneumatic push rod 4, a gas cylinder 5, a water tank 6 and a flushing pipe 7. The drug pushing cartridge 2 is arranged on the mounting bracket 1 along the up and down directions. A push rod 21 is slidably connected inside the drug pushing cartridge 2. The pneumatic push rod 4 is connected to the upper end of the push rod 21 and is used to push the push rod 21 downward. The gas cylinder 5 is connected to the pneumatic push rod 4 through the gas supply main pipe 51. The lower end of the drug pushing cartridge 2 is connected to the drug delivery head 3 through the liquid delivery pipe 23. The flushing pipe 7 is connected between the water tank 6 and the liquid delivery pipe 23. A control ball valve 22 is provided at the lower part of the drug pushing cartridge 2;

[0029] Among them, a branch pipe 52 is connected between the air supply main pipe 51 and the water tank 6, and an air flow control valve 53 is provided on the branch pipe 52. The water tank 6 can supply flushing water to the liquid delivery pipe 23 when the air flow control valve 53 is opened.

[0030] Compared with the prior art, the patch clamp dosing device provided in this embodiment uses a mounting bracket 1 to mount and fix the drug pusher cartridge 2, and uses a pneumatic push rod 4 to push and press down the push rod 21 of the drug pusher cartridge 2, so that the drug solution in the drug pusher cartridge 2 enters the liquid delivery tube 23, avoiding the problem of electromagnetic interference during the driving process. When the drug solution needs to be replaced, the air flow control valve 53 is opened to supply gas into the water tank 6, and then the water in the water tank 6 flows into the flushing tube 7 and the liquid delivery tube 23 under the action of air pressure, so as to flush the liquid delivery tube 23, facilitate the subsequent replacement of different drug solutions, avoid cross contamination during the drug change process, improve the accuracy of the test, and improve the operating efficiency.

[0031] In this embodiment, the lower opening of the drug pusher 2 is connected to the drug delivery head 3 through the liquid delivery tube 23, and the flushing tube 7 is connected between the water tank 6 and the liquid delivery tube 23. The above connection relationship and position setting avoid the waste of medicine caused by the excessive length of the pipeline. The flushing tube 7 is connected to the inlet end of the liquid delivery tube 23, which is convenient for the subsequent flushing of the liquid delivery tube 23 and also avoids the problem of drug waste. In addition, the method of driving the drug pusher 2 by the pneumatic push rod 4 can effectively solve the electromagnetic interference problem that is easily generated in the traditional driving process, ensure the accuracy of the experimental data, meet the cleaning requirements of the pipeline during the dressing change process, and improve the operating efficiency during the test process.

[0032] In one possible implementation, please also refer to Figures 1 to 6 There are several medicine push cartridges 2 and several pneumatic push rods 4 respectively, and several medicine push cartridges 2 are respectively arranged in one-to-one correspondence with several pneumatic push rods 4, and several pneumatic push rods 4 are arranged at intervals along the axial direction of the gas supply main pipe 51.

[0033] In this embodiment, in order to improve the supply efficiency of the liquid medicine, the liquid medicine can be supplied synchronously through multiple medicine pushing cylinders 2, and each corresponding liquid medicine cylinder is connected to a starting push rod 21, and the liquid medicine in each medicine withdrawal barrel is realized through the pushing action of the pneumatic push rod 4. It meets the supply demand of the liquid medicine and improves.

[0034] The time is shortened. Experimental operation efficiency

[0035] In some embodiments, please refer to Figures 1 to 6 The upper end of the pneumatic push rod 4 is connected to the gas supply main pipe 51 through the gas supply branch pipe 54. The gas supply branch pipe 54 is provided with a pneumatic throttle valve 541 and a pneumatic solenoid valve 542. The pneumatic throttle valve 541 is located above the pneumatic solenoid valve 542.

[0036] In this embodiment, the pneumatic solenoid valve 542 is used to control the gas supply of the pneumatic push rod 4. A gas pipeline is connected between the pneumatic solenoid valve 542 and the pneumatic push rod 4. The gas pipeline is provided with a pneumatic regulating joint 543 for controlling the flow rate of the gas pipeline, so as to make fine adjustments to the gas pressure and improve the accuracy of the pneumatic drive action. A pressure reducing valve is provided at the upper end of the gas cylinder 5, and the pressure reducing valve can adjust the pressure of the gas delivered by the gas cylinder 5 so that the gas pressure meets the pushing demand of the pneumatic push rod 4.

[0037] In some embodiments, please refer to Figures 1 to 6 The gas cylinder 5 is connected to the middle of the gas supply main pipe 51 through the gas supply pipe 55, and the branch pipe 52 is connected to the end of the gas supply main pipe 51. The outlet end of the gas supply pipe 55 is connected to the middle of the gas supply main pipe 51, so as to distribute the gas flow in an orderly manner at various axial locations of the gas supply main pipe 51, and then synchronously supply gas to multiple gas supply branch pipes 54.

[0038] The branch pipe 52 is connected to the end of the air supply main pipe 51, and can pressurize the water tank 6 when the air flow control valve 53 is opened, so that the water in the water tank 6 is sent to the liquid delivery pipe 23 through the flushing pipe 7, thereby achieving effective flushing of the liquid delivery pipe 23.

[0039] In one possible implementation, please also refer to Figures 1 to 6 The inlet end of the flushing pipe 7 is connected to the liquid delivery pipe 23 through a flushing branch 71, and a sub-control valve 72 is provided on the flushing branch 71. When the liquid delivery pipe 23 needs to be flushed, the control ball valve 22 on the drug pushing cylinder 2 is first closed to prevent water from entering the drug pushing cylinder 2, and then the sub-control valve 72 is opened, and the water in the flushing pipe 7 flows into the liquid delivery pipe 23 through the flushing branch, so as to flush the liquid delivery pipe 23, facilitate the subsequent replacement of the liquid medicine, and avoid cross contamination between different liquid medicines.

[0040] In one possible implementation, please also refer to Figures 1 to 6 A buffer damper 41 is provided between the medicine pushing barrel 2 and the pneumatic push rod 4, the upper end of the buffer damper 41 is connected to the pushing end of the pneumatic push rod 4, and the lower end of the buffer damper 41 is connected to the upper end of the push rod 21. The buffer damper 41 provided between the medicine pushing barrel 2 and the pneumatic push rod 4 is used for buffering, so as to avoid the rigid impact between the medicine pushing barrel 2 and the pneumatic push rod 4 affecting the uniformity of the medicine supply, thereby improving the accuracy of the test data.

[0041] In one possible implementation, please also refer to Figures 1 to 6The patch clamp dosing device also includes an operating cabinet 8, a mounting bracket 1 is connected to the inner wall of the operating cabinet 8, a drug pusher 2, a pneumatic push rod 4 and a drug delivery head 3 are respectively located in the operating cabinet 8, and a water tank 6 and a gas cylinder 5 are respectively arranged on the top of the operating cabinet 8. The operating cabinet 8 is used to accommodate the above components, so as to improve the cleanliness of the operating environment. The water tank 6 and the gas cylinder 5 are arranged on the top of the operating cabinet 8, which is convenient for the replenishment of water and gas. The front side of the operating cabinet 8 is hinged with a transparent door, which is convenient for replacing the internal components of the operating cabinet 8 and for observing the operation of the equipment, and has good practicality.

[0042] In one possible implementation, please also refer to Figures 1 to 6 The mounting bracket 1 includes a frame body 11, a first transverse plate 12 and a second transverse plate 13. The first transverse plate 12 and the second transverse plate 13 are respectively connected to the front side of the frame body 11. The first transverse plate 12 is provided with a first card groove 14 which is clamped with the cylinder body of the pushing cartridge 2. The first transverse plate 12 is used to support the lower part of the upper end plate of the pushing cartridge 2. The second transverse plate 13 is located below the first transverse plate 12. The second transverse plate 13 is provided with a second card groove 15 which is clamped with the cylinder body of the pushing cartridge 2. The second card groove 15 is arranged corresponding to the first card groove 14 up and down, and the second card groove 15 is clamped on the lower outer periphery of the pushing cartridge 2.

[0043] In this embodiment, the peripheral wall of the drug pusher 2 is clamped and installed by utilizing the first clamping groove 14 on the first transverse plate 12 and the second clamping groove 15 on the second transverse plate 13. An upper end plate is provided at the upper end of the drug pusher 2. The upper end plate can be supported on the top of the first transverse plate 12, so that the axial position of the drug pusher 2 is further limited, thereby meeting the reliability of its installation.

[0044] In some embodiments, please refer to Figures 1 to 6 The frame 11 is provided with a limit column 16 extending in the up-down direction, and the two sides of the push cartridge 2 are symmetrically provided with engaging claws 24 that can be clamped on both sides of the limit column 16, and the extending ends of the two engaging claws 24 extend to the adjacent sides.

[0045] In this embodiment, a limiting column 16 is connected between the first horizontal plate 12 and the second horizontal plate 13, and the engaging claws 24 on both sides of the push cartridge 2 can engage with both sides of the outer periphery of the limiting column 16, so that the installation position of the push cartridge 2 is further limited, thereby ensuring the stability of the installation position of the push cartridge 2.

[0046] In one possible implementation, please also refer to Figures 1 to 6A liquid medicine collecting seat 9 is provided above the medicine delivery head 3, which is connected to the liquid outlet pipe of the liquid delivery pipe 23 and is used to collect the liquid medicine in the liquid delivery pipe 23. A collecting cavity connected to the medicine delivery head 3 is provided in the liquid medicine collecting seat 9. The liquid medicine collecting box provided above the medicine delivery head 3 can centrally collect the liquid medicine in multiple liquid delivery pipes 23 and uniformly deliver them through the medicine delivery head 3, thereby realizing the centralized supply of liquid medicine, helping to reduce the operation time and improve the convenience of operation.

[0047] The above-mentioned patch clamp dosing device uses the mounting bracket 1 to install and fix the push cartridge 2, and uses the pneumatic push rod 4 to push and press the push rod 21 of the push cartridge 2, so that the medicine in the push cartridge 2 enters the liquid delivery tube 23, avoiding the problem of electromagnetic interference during the driving process. When the medicine needs to be replaced, the air flow control valve 53 is opened to supply gas into the water tank 6, and then the water in the water tank 6 flows into the flushing tube 7 and the liquid delivery tube 23 under the action of air pressure, so as to flush the liquid delivery tube 23, facilitate the subsequent replacement of different medicines, avoid cross contamination during the medicine change process, improve the accuracy of the test, and improve the operating efficiency.

[0048] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A patch clamp dosing device, characterized in that: It includes a mounting bracket, a drug-pushing cartridge, a drug-delivering head, a pneumatic push rod, a gas cylinder, a water tank and a flushing pipe. The drug-pushing cartridge is arranged on the mounting bracket in the up-down direction. A push rod is slidably connected in the drug-pushing cartridge. The pneumatic push rod is connected to the upper end of the push rod and is used to push the push rod downward. The gas cylinder is connected to the pneumatic push rod through an air supply main pipe. The lower end of the drug-pushing cartridge is connected to the drug-delivering head through a liquid delivery pipe. The flushing pipe is connected between the water tank and the liquid delivery pipe. A control ball valve is provided at the lower part of the drug-pushing cartridge. Wherein, a branch pipe is connected between the gas supply main pipe and the water tank, and an air flow control valve is provided on the branch pipe. The water tank can supply flushing water to the liquid delivery pipe when the air flow control valve is opened.

2. The patch clamp dosing device according to claim 1, characterized in that: There are several medicine pushing cartridges and several pneumatic push rods respectively, several medicine pushing cartridges and several pneumatic push rods are respectively arranged in one-to-one correspondence, and several pneumatic push rods are arranged at intervals along the axial direction of the gas supply main pipe.

3. The patch clamp dosing device according to claim 2, characterized in that: The upper end of the pneumatic push rod is connected to the gas supply main pipe through an air supply branch pipe, and a pneumatic throttle valve and a pneumatic solenoid valve are arranged on the air supply branch pipe. The pneumatic throttle valve is located above the pneumatic solenoid valve.

4. The patch clamp dosing device according to claim 3, characterized in that: The gas cylinder is communicated with the middle part of the gas supply main pipe through the gas delivery pipe, and the branch pipe is connected to the end of the gas supply main pipe.

5. The patch clamp dosing device according to claim 2, characterized in that: The flushing pipe is connected to the inlet end of the liquid delivery pipe through a flushing branch, and a sub-control valve is provided on the flushing branch.

6. The patch clamp dosing device according to claim 1, characterized in that: A buffer damper is provided between the medicine pushing cartridge and the pneumatic push rod, the upper end of the buffer damper is connected to the pushing end of the pneumatic push rod, and the lower end of the buffer damper is connected to the upper end of the push rod.

7. The patch clamp dosing device according to claim 1, characterized in that: The patch clamp dosing device also includes an operating cabinet, the mounting bracket is connected to the inner wall of the operating cabinet, the drug pushing cylinder, the pneumatic push rod and the drug delivery head are respectively located in the operating cabinet, and the water tank and the gas cylinder are respectively arranged on the top of the operating cabinet.

8. The patch clamp dosing device according to any one of claims 1 to 7, characterized in that: The mounting bracket includes a frame body, a first transverse plate and a second transverse plate, the first transverse plate and the second transverse plate are respectively connected to the front side of the frame body, the first transverse plate is provided with a first slot that is clamped with the barrel of the pushing cartridge, the first transverse plate is used to support the bottom of the upper end plate of the pushing cartridge, the second transverse plate is located below the first transverse plate, the second transverse plate is provided with a second slot that is clamped with the barrel of the pushing cartridge, the second slot is arranged corresponding to the first slot up and down, and the second slot is clamped to the lower outer periphery of the pushing cartridge.

9. The patch clamp dosing device according to claim 8, characterized in that: The frame is provided with a limit column extending in the up-down direction, and the two sides of the drug pushing cartridge are symmetrically provided with engaging claws that can be clamped on both sides of the limit column, and the extending ends of the two engaging claws extend to adjacent sides.

10. The patch clamp dosing device according to any one of claims 1 to 7, characterized in that: A liquid medicine collecting seat connected to the liquid feeding tube and used for collecting liquid medicine in the liquid feeding tube is provided above the medicine feeding head, and a collecting cavity connected to the medicine feeding head is provided in the liquid medicine collecting seat.

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