Cooling device used in stem cell cryopreservation process

Abstract

The utility model belongs to the field of cooling devices, and particularly relates to a cooling device used in the stem cell cryopreservation process. The technology comprises a storage tank, the top of the storage tank is provided with a tank cover for sealing the storage tank, a partition plate is fixed in the storage tank and is of a tubular structure integrally, a thermal insulation layer is arranged between the partition plate and the storage tank, and a fixing plate is horizontally fixed to the bottom in the partition plate and divides the partition plate into a first area and a second area from top to bottom; a vertically-through channel is formed in the fixing plate, an adjusting assembly is arranged in the channel, and a liquid injection assembly is arranged on the outer side wall of the storage tank. By means of the design, the stem cell cryopreservation tube can be conveniently and rapidly placed in the storage tank to be cooled through liquid nitrogen, and meanwhile operators can be conveniently prevented from being injured when the stem cell cryopreservation tube is stored and taken out.

Description

Technical Field

[0001] The utility model belongs to the field of cooling devices, and particularly relates to a cooling device used in the process of cryopreserving stem cells. Background Art

[0002] Stem cells are a class of pluripotent cells with self-renewal ability. Stem cells need to be mixed during the process of slow cooling, so that the stem cells and the cryopreservation solution are mixed evenly while cooling.

[0003] Usually, stem cells are first placed in a cryopreservation tube and then cooled with liquid nitrogen. However, the temperature of liquid nitrogen is very low, and it is easy for liquid nitrogen to overflow from the container during the process of taking out and placing. Once the operation is improper, the operator will be frostbitten, which makes it dangerous for the operator to take out and place the stem cell test tube, and at the same time, the efficiency of storing and taking the stem cell cryopreservation tube is low. Content of the Utility Model

[0004] The purpose of the utility model is to provide a cooling device used in the process of cryopreserving stem cells, which is convenient for reducing the risk of storing and taking stem cells by operators, and improving the working efficiency at the same time.

[0005] The cooling device used in the process of cryopreserving stem cells includes a storage tank. A tank cover for closing it is arranged at the top of the storage tank. A partition is fixed in the storage tank, and the partition is integrally in a tubular structure. A heat insulation layer is arranged between the partition and the storage tank. A fixing plate is horizontally fixed at the inner bottom of the partition. The fixing plate divides the inside of the partition into a first area and a second area from top to bottom. The first area is used to place the stem cell cryopreservation tube, and the second area is used to inject liquid nitrogen. A channel communicating up and down is opened on the fixing plate, and an adjusting component for preventing liquid nitrogen from entering the first area from the second area is arranged in the channel. When the stem cell cryopreservation tube is located in the partition and the heat insulation layer closes the storage tank, the first area and the second area are connected and communicated. A liquid injection component for injecting liquid nitrogen into the second area is arranged on the outer side wall of the storage tank.

[0006] Further, the adjusting component includes at least two fixing rods, and the channel is correspondingly opened into two for the fixing rods. The fixing rods are independently inserted into the channel. A placing plate is horizontally fixed at the top of the fixing rod above the fixing plate. A spring is sleeved on the fixing rod between the placing plate and the fixing plate. First through holes for liquid nitrogen to pass through are opened on both the placing plate and the fixing plate. A baffle is horizontally fixed at the bottom of the fixing rod below the fixing plate. A slot for the baffle to insert is opened at the bottom of the fixing plate, and a sealing ring is arranged in the slot. In the natural state, the top of the baffle is closely attached to the sealing ring.

[0007] Further, the liquid injection assembly includes a connecting pipe. Second through holes that communicate with each other left and right are provided in the storage tank, the partition plate, and the heat insulation layer. The connecting pipe is hermetically inserted through the second through hole, and a sealing plug for closing it is detachably arranged on the connecting pipe located outside the storage tank.

[0008] Further, the second through hole is inclined with the outer side higher and the inner side lower, and the horizontal height of the highest point of the second through hole is lower than the lowest point of the baffle in the natural state.

[0009] Further, the tank cover is in threaded fit with the storage tank, and a groove for the top of the stem cell cryopreservation tube to be inserted is provided at the bottom of the tank cover, and a groove body that can rotate freely left and right with it is arranged in the groove.

[0010] Further, sliding blocks are fixed on the left and right side walls of the placement plate, sliding grooves for the sliding blocks to slide up and down are vertically provided on the left and right side walls of the partition plate, and the horizontal height of the top of the fixing plate is lower than the lowest point horizontal height of the sliding grooves.

[0011] Further, the heat insulation layer is made of rigid polyurethane foam material.

[0012] Compared with the prior art, the utility model has the following beneficial effects:

[0013] Through the arrangement of the partition plate and the heat insulation layer, the utility model is convenient for avoiding frostbite caused by the too low temperature of the storage tank when the operator touches it; furthermore, through the arrangement of the first area, the second area and the adjustment assembly, when the stem cell cryopreservation tube is located inside the partition plate and the heat insulation layer closes the storage tank, the first area and the second area are connected and communicated, which is convenient for avoiding injury to the operator caused by the leakage of liquid nitrogen when opening the tank cover to place the stem cell cryopreservation tube, thereby reducing the risk while effectively improving the efficiency. Through the arrangement of the liquid injection assembly, it is convenient to inject and extract liquid nitrogen, so that while cooling the stem cell cryopreservation tube, when taking out the stem cell cryopreservation tube, the liquid nitrogen in the storage tank can be first extracted through the liquid injection assembly to avoid frostbite to the operator when taking out the stem cell cryopreservation tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 is a schematic structural diagram of the utility model;

[0015] Figure 2 is Figure 1 the enlarged view at A in

[0016] Figure 3 is a diagram of the utility model in the use state;

[0017] Figure 4 is Figure 3 the enlarged view at B in

[0018] Names of each component in the figure: 1. Storage tank; 2. Partition board; 3. Heat insulation layer; 4. Tank cover; 5. Tank body; 6. Object placement board; 7. Sealing plug; 8. Connecting pipe; 9. Baffle; 10. Fixed plate; 11. Spring; 12. Fixed rod; 13. Sealing ring; 14. Slide block. Detailed implementation manners

[0019] The following further illustrates the present utility model through specific embodiments in conjunction with the accompanying drawings, but it is not used to limit the present utility model. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present utility model shall be included within the protection scope of the present invention.

[0020] Embodiment 1

[0021] A cooling device used during the cryopreservation of stem cells described in this embodiment, as Figure 1 shown, includes a storage tank 1. A tank cover 4 for closing it is provided at the top of the storage tank 1, which is convenient for closing the storage tank 1 after putting the stem cell cryopreservation tube into it, making its heat preservation better and at the same time avoiding the leakage of liquid nitrogen.

[0022] A partition board 2 is fixed inside the storage tank 1, and the partition board 2 is integrally in a tubular structure. A heat insulation layer 3 is provided between the partition board 2 and the storage tank 1, which is convenient for carrying the storage tank 1 and avoiding frostbite when the operator touches the storage tank 1 due to the too low internal temperature of the storage tank 1.

[0023] A fixed plate 10 is horizontally fixed at the inner bottom of the partition board 2. The fixed plate 10 divides the inside of the partition board 2 into a first area and a second area from top to bottom. The first area is used for placing the stem cell cryopreservation tube, and the second area is used for injecting liquid nitrogen. A channel communicating up and down is opened on the fixed plate 10, and an adjusting component for preventing liquid nitrogen from entering the first area from the second area is arranged in the channel. When the stem cell cryopreservation tube is located inside the partition board 2 and the heat insulation layer 3 closes the storage tank 1, the first area and the second area are connected and communicated, which is convenient for avoiding the leakage of liquid nitrogen when opening the tank cover 4 to place the stem cell cryopreservation tube and causing injury to the operator, thereby reducing the risk and effectively improving the efficiency at the same time.

[0024] A liquid injection component for injecting liquid nitrogen into the second area is arranged on the outer side wall of the storage tank 1, which is convenient for injecting and extracting liquid nitrogen. Thus, while cooling the stem cell cryopreservation tube, when taking out the stem cell cryopreservation tube, the liquid nitrogen in the storage tank 1 can be first extracted through the liquid injection component, and then the tank cover 4 can be opened to take out the stem cell cryopreservation tube, thereby avoiding frostbite to the operator when taking out.

[0025] Embodiment 2

[0026] This embodiment further illustrates the technology, as Figure 1 , Figure 2 , Figure 3 andFigure 4 As shown in the figure, the adjusting assembly includes at least two fixing rods 12, and the channels are correspondingly opened into two for the fixing rods 12. The fixing rods 12 are independently inserted into the channels. At the top of the fixing rod 12 above the fixing plate 10, a placing plate 6 is horizontally fixed. A spring 11 is sleeved on the fixing rod 12 between the placing plate 6 and the fixing plate 10. Both the placing plate 6 and the fixing plate 10 are provided with first through holes for liquid nitrogen to pass through. At the bottom of the fixing rod 12 below the fixing plate 10, a baffle 9 is horizontally fixed. A slot for the baffle 9 to be inserted is opened at the bottom of the fixing plate 10, and a sealing ring 13 is arranged in the slot. In the natural state, the top of the baffle 9 is in close contact with the sealing ring 13, so that there is no liquid nitrogen in the first area before the cryopreservation tube is placed, and thus no liquid nitrogen will leak out to cause harm to the operator when the tank cover 4 is opened to place the stem cell cryopreservation tube;

[0027] The adjusting assembly includes three fixing rods 12, and the channels are correspondingly opened into three for the fixing rods 12. The fixing rods 12 are independently inserted into the channels. At the top of the fixing rod 12 above the fixing plate 10, a placing plate 6 is horizontally fixed. A spring 11 is sleeved on the fixing rod 12 between the placing plate 6 and the fixing plate 10. Both the placing plate 6 and the fixing plate 10 are provided with first through holes for liquid nitrogen to pass through. At the bottom of the fixing rod 12 below the fixing plate 10, a baffle 9 is horizontally fixed. A slot for the baffle 9 to be inserted is opened at the bottom of the fixing plate 10, and a sealing ring 13 is arranged in the slot. In the natural state, the top of the baffle 9 is in close contact with the sealing ring 13, and the baffle 9 is made of rubber material, so that there is no liquid nitrogen in the first area before the cryopreservation tube is placed, and thus no liquid nitrogen will leak out to cause harm to the operator when the tank cover 4 is opened to place the stem cell cryopreservation tube. At the same time, it is also convenient to increase the degree of fit between the baffle 9 and the slot, so as to improve the sealing effect and reduce the leakage of liquid nitrogen.

[0028] In order to better use the spring 11 in the low-temperature environment of liquid nitrogen, stainless steel springs and alloy steel springs are used

[0029] Embodiment 3

[0030] This embodiment will further illustrate the technology, as Figure 1 As shown in the figure, the liquid injection assembly includes a connecting pipe 8. Second through holes that communicate with each other left and right are opened in the storage tank 1, the partition plate 2 and the heat insulation layer 3. The connecting pipe 8 is hermetically inserted into the second through holes. A sealing plug 7 that closes it is detachably arranged on the connecting pipe 8 outside the storage tank 1, which is convenient for injecting liquid nitrogen into the second area, then convenient for placing the stem cell cryopreservation tube into the storage tank 1 for cooling, and also convenient for first pumping out the liquid nitrogen and then taking out the stem cell cryopreservation tube, so as to avoid the operator from being injured.

[0031] Embodiment 4

[0032] This embodiment further illustrates the technology. The second through hole is inclined with the outer part higher and the inner part lower, which facilitates the rapid injection of liquid nitrogen into the second area and can, to a certain extent, prevent overflow during the injection process.

[0033] In the natural state, the horizontal height of the highest point of the second through hole is lower than the lowest point of the baffle 9, which helps to prevent liquid nitrogen from entering the first area through the gap between the baffle 9 and the fixing plate 10 during the injection.

[0034] Embodiment 5

[0035] This embodiment further illustrates the technology. As Figure 1 shown, the tank cover 4 is in threaded fit with the storage tank 1, and a groove for the top of the stem cell cryopreservation tube to insert is provided at the bottom of the tank cover 4. A slot 5 that can rotate freely left and right is arranged in the groove, which facilitates the downward movement of the stem cell cryopreservation tube driving the object placing plate 6 while rotating the connection between the tank cover 4 and the storage tank 1, so that the baffle 9 disengages from the slot to connect the first area and the second area, thus making it more labor-saving for the operator.

[0036] Embodiment 6

[0037] This embodiment further illustrates the technology. As Figure 2 and Figure 4 shown, sliding blocks 14 are fixed on the left and right side walls of the object placing plate 6. Slide grooves for the sliding blocks 14 to slide up and down are vertically provided on the left and right side walls of the partition plate 2, and the horizontal height of the top of the fixing plate 10 is lower than the lowest point horizontal height of the slide grooves, which helps to make the up and down sliding of the object placing plate 6 more stable and makes the operator's work easier.

[0038] Embodiment 7

[0039] This embodiment further illustrates the technology. As Figure 1 and Figure 3 shown, the heat insulation layer 3 is made of rigid polyurethane foam material, which helps to effectively isolate the low temperature of liquid nitrogen and prevent the operator from being injured or feeling uncomfortable due to the too low temperature when touching the storage tank 1.

[0040] The heat insulation layer 3 can also be made of polyurethane foam, polystyrene, aluminum silicate fiber felt or aerogel material.

[0041] Working principle: First, inject liquid nitrogen into the second area through the connecting pipe 8, then seal it with the sealing plug 7. After that, open the tank cover 4, put the stem cell cryopreservation tube into the storage tank 1, and then fasten the tank cover 4 on the top of the storage tank 1 and the stem cell cryopreservation tube. Then press to connect the tank cover 4 with the top of the storage tank 1. After the tank cover 4 is threadedly connected to the top of the storage tank 1, it is convenient to drive the placement plate 6 to move downward while rotating the connection between the tank cover 4 and the storage tank 1, so that the baffle 9 disengages from the slot to connect the first area and the second area, enabling the liquid nitrogen in the second area to enter the first area through the first through holes on the fixing plate 10 and the placement plate 6 to cool the stem cell cryopreservation tube; when taking out the stem cell cryopreservation tube, first pump out the liquid nitrogen through the connecting pipe 8, and then open the tank cover 4. During the opening process of the tank cover 4, driven by the spring 11, the stem cell cryopreservation tube pops out of the storage tank 1, and then the stem cell cryopreservation tube can be taken out.

Claims

1. A cooling device used in the cryopreservation of stem cells, comprising a storage tank (1), the top of which is provided with a tank cover (4) for sealing the storage tank (1), characterized in that: A partition (2) is fixed inside the storage tank (1), and the partition (2) is of tubular structure as a whole. A thermal insulation layer (3) is arranged between the partition (2) and the storage tank (1). A fixed plate (10) is horizontally fixed to the bottom of the partition (2). The fixed plate (10) divides the inside of the partition (2) into a first area and a second area from top to bottom. The first area is used for placing a stem cell cryopreservation tube, and the second area is used for injecting liquid nitrogen. A channel communicating with each other from top to bottom is opened on the fixed plate (10). A regulating component for preventing liquid nitrogen from entering the first area from the second area is arranged in the channel. When the stem cell cryopreservation tube is located in the partition (2) and the thermal insulation layer (3) closes the storage tank (1), the first area and the second area are connected and communicated. An injection component for injecting liquid nitrogen into the second area is arranged on the outer wall of the storage tank (1).

2. The cooling device used in the cryopreservation of stem cells according to claim 1, characterized in that: The adjustment assembly comprises at least two fixing rods (12), and two channels are formed corresponding to the fixing rods (12). The fixing rods (12) are independently installed in the channels. A storage plate (6) is horizontally fixed to the top of the fixing rod (12) located above the fixing plate (10). A spring (11) is sleeved on the fixing rod (12) between the storage plate (6) and the fixing plate (10). A first through hole for liquid nitrogen to pass through is formed on both the storage plate (6) and the fixing plate (10). A baffle (9) is horizontally fixed to the bottom of the fixing rod (12) located below the fixing plate (10). A slot for inserting the baffle (9) is formed at the bottom of the fixing plate (10), and a sealing ring (13) is provided in the slot. In a natural state, the top of the baffle (9) is tightly fitted with the sealing ring (13).

3. The cooling device used in the cryopreservation of stem cells according to claim 2, characterized in that: The liquid injection assembly comprises a connecting pipe (8), a second through hole communicating left and right is opened on the storage tank (1), the partition (2) and the thermal insulation layer (3), the connecting pipe (8) is sealed and inserted into the second through hole, and a sealing plug (7) for sealing the connecting pipe (8) located outside the storage tank (1) is detachably provided.

4. The cooling device used in the cryopreservation of stem cells according to claim 3, characterized in that: The second through hole is in an inclined shape with a high outside and a low inside, and in a natural state, the horizontal height of the highest point of the second through hole is lower than the lowest point of the baffle (9).

5. The cooling device used in the cryopreservation of stem cells according to claim 1, characterized in that: The tank cover (4) is threadably matched with the storage tank (1), and a groove for inserting the top of the stem cell cryopreservation tube is provided at the bottom of the tank cover (4), and a groove body (5) is provided in the groove so as to freely rotate leftward and rightward with the groove.

6. The cooling device used in the cryopreservation of stem cells according to claim 2, characterized in that: The left and right side walls of the storage plate (6) are fixed with sliders (14), and the left and right side walls of the partition plate (2) are vertically provided with sliding grooves for the sliders (14) to slide up and down, and the horizontal height of the top of the fixed plate (10) is lower than the horizontal height of the lowest point of the sliding groove.

7. The cooling device for use in the cryopreservation of stem cells according to claim 2, characterized in that: The thermal insulation layer (3) is made of hard polyurethane foam material.

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