Stem cell exosome enrichment device

Abstract

The utility model relates to the technical field of exosome enrichment, in particular to a stem cell exosome enrichment device which comprises a centrifugal machine body, an operation center is arranged on the front face of the centrifugal machine body, a protective cover covers the operation end of the operation center, and a machine cover is hinged to the upper portion of the centrifugal machine body. The rotating shaft is arranged at the driving end of the centrifugal machine body, a rotor seat is connected to the top of the rotating shaft, a pressing plate is hinged to the end of the rotor seat through a hinge, and hand screws are connected to the interiors of the rotor seat and the pressing plate. Through the arrangement of the protective cover, the operation screen of the device can be covered and protected, collision of the screen in the idle process of the device is avoided, meanwhile, through the arrangement of the split type rotor base, the centrifugal cup can be conveniently and rapidly disassembled and assembled, the disassembling and assembling convenience of the centrifugal cup is improved, and the working efficiency of the centrifugal cup is improved. And meanwhile, switching treatment of different types of centrifugal cups can be achieved, and the adaptability and flexibility of the device in use are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of exosome enrichment, in particular to a device for enriching stem cell exosomes. Background Technique

[0002] The separation and enrichment of exosomes mainly rely on traditional methods such as differential ultracentrifugation, density gradient ultracentrifugation, filtration, ultrafiltration, the combination of ultrafiltration and ultracentrifugation, and kit extraction. Among them, the centrifuge is one of the commonly used devices for its enrichment treatment.

[0003] When the existing centrifuge for exosome enrichment is in operation, according to different treatment situations, centrifuge cups of different models need to be installed on the rotor seat to achieve centrifugation operations for different containers. However, the setting of the integral structure rotor seat makes it inconvenient to disassemble the centrifuge cup, and only the replacement of the internal partition can be realized, thus reducing the convenience of changing the centrifuge cup model and making it inconvenient for rapid operation of model switching, thereby reducing the adaptability and flexibility of the device in use. Summary of the Utility Model

[0004] The purpose of the utility model is to provide a device for enriching stem cell exosomes to solve the problem that the setting of the integral structure rotor seat in the above-mentioned background technique makes it inconvenient to disassemble the centrifuge cup, thereby reducing the convenience of changing the centrifuge cup model and making it inconvenient for rapid operation of model switching.

[0005] To achieve the above purpose, the utility model provides the following technical solution: A device for enriching stem cell exosomes, comprising:

[0006] A centrifuge body, an operation center is arranged on the front of the centrifuge body, and a protective cover covers the operation end of the operation center. A machine cover is hinged above the centrifuge body;

[0007] A rotating shaft, the rotating shaft is arranged at the driving end of the centrifuge body. The top of the rotating shaft is connected with a rotor seat. One end of the rotor seat is hinged with a pressing plate through a hinge. The inside of the rotor seat and the pressing plate is connected with a hand-tightening screw. A pin shaft is clamped between the rotor seat and the pressing plate. One end of the pin shaft is fixedly connected with a centrifuge cup, and both ends of the centrifuge cup are connected with pin shafts.

[0008] Preferably, the centrifuge body and the machine cover are rotatably connected through a hinge, and anti-slip foot pads are arranged at the bottom of the centrifuge body.

[0009] Preferably, the center of the protective cover is made of transparent acrylic material. A first magnetic strip is arranged on one side of the non-hinged end of the protective cover close to the operation center, and a second magnetic strip is arranged on one side of the operation center close to the first magnetic strip.

[0010] Preferably, the branches of the rotor seat are in a Y-shaped structure, and a set of centrifuge cups are hung between adjacent Y-shaped structures.

[0011] Preferably, the top of the rotating shaft is in a stepped shape, and a nut is threadedly connected to the outer part of the stepped shape. A horizontal abutting surface is provided on the outer wall of the stepped shape at the top of the rotating shaft.

[0012] Preferably, the center of the rotor seat is sleeved and engaged with the stepped shape of the rotating shaft. Through holes for adapting to the pin shafts are provided at the Y-shaped structure of the rotor seat.

[0013] Preferably, threaded holes are provided at the positions of the rotor seat corresponding to the hinge steps. Positioning holes are provided at the corresponding positions of the hinge. A hand-tightening screw penetrates through the positioning hole into the threaded hole. External threads adapted to the threaded holes are provided on the outer wall of the hand-tightening screw.

[0014] Compared with the prior art, the beneficial effects of the present utility model are as follows: Through the setting of the protective cover, the operation screen of the device can be covered and protected, avoiding the screen from being knocked during its idle process. At the same time, the setting of the split-type rotor seat can facilitate the quick disassembly and assembly of the centrifuge cup, thereby improving the convenience of the disassembly and assembly of the centrifuge cup. At the same time, the switching process of centrifuge cups of different models can be realized, improving the adaptability and flexibility of the device in use.

[0015] (1) Through the setting of the rotor seat, the pressing plate and the hand-tightening screw in the present utility model, the switching process of the centrifuge cup can be facilitated, thereby improving the adaptability and flexibility of the device in use. At the same time, through the clamping and positioning at its end, the convenience of its disassembly and assembly is realized.

[0016] (2) Through the setting of the protective cover in the present utility model, the operation screen of the device can be covered and protected, thereby improving the safety of the device in use, avoiding accidental external knocking of the operation screen during its idle process. At the same time, the setting of the transparent material at the center is convenient for observing the data on the screen. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 is the overall structural schematic diagram of the present utility model;

[0018] Figure 2 is the sectional structural schematic diagram of the present utility model;

[0019] Figure 3 is the Figure 2 enlarged structural schematic diagram of A in the present utility model;

[0020] Figure 4 is the exploded structural schematic diagram of the rotor seat of the present utility model;

[0021] Figure 5 is the Figure 4Schematic diagram of the enlarged structure of B.

[0022] In the figure: 1. Centrifuge body; 2. Machine cover; 3. Operation center; 31. Protective cover; 32. First magnetic strip; 33. Second magnetic strip; 4. Rotating shaft; 41. Rotor seat; 42. Hinge; 43. Pressing plate; 44. Hand-tightening screw; 45. Centrifugal cup; 46. Pin shaft. Specific implementation mode

[0023] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0024] Please refer to Figures 1-5 , an embodiment provided by the present invention: A stem cell exosome enrichment device, comprising:

[0025] A centrifuge body 1, an operation center 3 is arranged on the front surface of the centrifuge body 1, and the operation end of the operation center 3 is covered with a protective cover 31. A machine cover 2 is hinged above the centrifuge body 1. The specific model of the centrifuge body 1 is TDD5KR;

[0026] A rotating shaft 4, the rotating shaft 4 is arranged at the driving end of the centrifuge body 1. The top of the rotating shaft 4 is connected with a rotor seat 41. The end of the rotor seat 41 is hinged with a pressing plate 43 through a hinge 42. The inside of the rotor seat 41 and the pressing plate 43 is connected with a hand-tightening screw 44. A pin shaft 46 is clamped between the rotor seat 41 and the pressing plate 43. The end of the pin shaft 46 is fixedly connected with a centrifugal cup 45, and both ends of the centrifugal cup 45 are connected with a pin shaft 46. Through the setting of this structure, by loosening the hand-tightening screw 44, the pressing plate 43 can be flipped, and then the pressing on the pin shaft 46 can be released. At this time, the centrifugal cup 45 can be disassembled and replaced, so as to realize the use of different models of centrifugal cups 45 and improve the adaptability of the device.

[0027] Furthermore, the centrifuge body 1 and the machine cover 2 are rotatably connected through a hinge. Anti-slip foot pads are arranged at the bottom of the centrifuge body 1 to increase the friction of its placement and ensure the stability of its placement.

[0028] Furthermore, the center of the protective cover 31 is made of transparent acrylic material. A first magnetic strip 32 is provided on one side of the non-hinged end of the protective cover 31 close to the operation center 3, and a second magnetic strip 33 is provided on one side of the operation center 3 close to the first magnetic strip 32. Through the covering of the protective cover 31, the safety protection of the operation center 3 is realized. At the same time, the setting of the transparent acrylic material in the center facilitates the observation of the data on the operation center 3. Through the magnetic attraction setting of the first magnetic strip 32 and the second magnetic strip 33, the magnetic attraction stability after the protective cover 31 is covered is improved.

[0029] Furthermore, the branches of the rotor seat 41 are in a Y-shaped structure, and a group of centrifuge cups 45 are hung between adjacent Y-shaped structures. The top of the rotating shaft 4 is in a stepped shape, and the external thread of the stepped shape is connected with a nut. A horizontal abutting surface is provided on the outer wall of the stepped shape at the top of the rotating shaft 4. The center of the rotor seat 41 is sleeved and engaged with the stepped shape of the rotating shaft 4. Through holes for adapting the pin shaft 46 are provided at the Y-shaped structure of the rotor seat 41. Through the mutual cooperation of the rotor seat 41 and the pressing plate 43, the covering and positioning of the pin shaft 46 can be completed, and then the supporting and positioning of the centrifuge cup 45 can be completed. Moreover, the disassembly and assembly method is simple and convenient, which can facilitate the model switching process of the centrifuge cup 45.

[0030] Furthermore, threaded holes are provided at the steps of the hinge 42 corresponding to the rotor seat 41, positioning holes are provided at the hinge 42 corresponding to the threaded holes, and a hand-tightening screw 44 penetrates through the positioning holes to the inside of the threaded holes. External threads adapted to the threaded holes are provided on the outer wall of the hand-tightening screw 44. Through the threaded connection of the hand-tightening screw 44, the pressing and positioning of the pressing plate 43 can be facilitated. Then, through the cooperation of the pressing plate 43 and the rotor seat 41, the limit installation of the pin shaft 46 can be completed, and thus the hanging installation of the centrifuge cup 45 can be completed.

[0031] Working principle: The centrifuge body 1, the operation center 3, and the rotating shaft 4 used in this application are all products that can be directly purchased on the market. Their principles and connection methods are all well-known prior arts to those skilled in the art, so they will not be elaborated here. When this utility model is in use, first select and install the model of the centrifuge cup 45 according to the operation type, etc. First, release the pressing and positioning of the hand-tightening screw 44, and then through the rotation of the pressing plate 43, the disassembly and assembly of the centrifuge cup 45 can be realized. Further, insert the pin shaft 46 on the outer wall of the new centrifuge cup 45 into the corresponding through hole of the rotor seat 41. Through the rotation of the pressing plate 43, the pin shaft 46 can be tightened. With the cooperation of the pressing and positioning of the hand-tightening screw 44, the stable installation of the pressing plate 43 can be completed, and thus the installation of the centrifuge cup 45 can be completed. After the sample is placed, close the machine cover 2 and start the device to perform the centrifugation operation.

[0032] For those skilled in the art, it is obvious that the present utility model is not limited to the details of the above-mentioned exemplary embodiments, and the present utility model can be implemented in other specific forms without departing from the spirit or basic characteristics of the present utility model. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting. The scope of the present utility model is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be embraced within the present utility model. Any reference signs in the claims should not be construed as limiting the claims involved.

Claims

1. A stem cell exosome enrichment device, characterized in that: include: A centrifuge body (1), wherein an operation center (3) is arranged on the front of the centrifuge body (1), and an operation end of the operation center (3) is covered with a protective cover (31), and a cover (2) is hingedly connected to the top of the centrifuge body (1); A rotating shaft (4) is arranged at the driving end of the centrifuge body (1), the top of the rotating shaft (4) is connected to a rotor seat (41), the end of the rotor seat (41) is hingedly connected to a pressure plate (43) through a hinge (42), the rotor seat (41) and the pressure plate (43) are internally connected with a hand screw (44), a pin shaft (46) is engaged between the rotor seat (41) and the pressure plate (43), the end of the pin shaft (46) is fixedly connected to a centrifugal cup (45), and both ends of the centrifugal cup (45) are connected to the pin shaft (46).

2. A stem cell exosome enrichment device according to claim 1, characterized in that: The centrifuge body (1) and the cover (2) are rotatably connected via a hinge, and a non-slip foot pad is provided at the bottom of the centrifuge body (1).

3. A stem cell exosome enrichment device according to claim 1, characterized in that: The center of the protective cover (31) is made of a transparent acrylic material, a first magnetic strip (32) is arranged on a side of the non-hinged end of the protective cover (31) close to the operation center (3), and a second magnetic strip (33) is arranged on a side of the operation center (3) close to the first magnetic strip (32).

4. A stem cell exosome enrichment device according to claim 1, characterized in that: The branches of the rotor seat (41) are in a Y-shaped structure, and a group of centrifugal cups (45) are hung between adjacent Y-shaped structures.

5. The stem cell exosome enrichment device according to claim 1, characterized in that: The top of the rotating shaft (4) is in a step shape, and the stepped external thread is connected to a nut, and the stepped outer wall at the top of the rotating shaft (4) is provided with a horizontal abutment surface.

6. A stem cell exosome enrichment device according to claim 5, characterized in that: The center of the rotor seat (41) and the rotating shaft (4) are mutually sleeved and engaged in a step-like manner, and a through hole for adapting to the pin shaft (46) is opened at the Y-shaped structure of the rotor seat (41).

7. A stem cell exosome enrichment device according to claim 5, characterized in that: A threaded hole is provided at the rotor seat (41) at the step corresponding to the hinge (42), a positioning hole is provided at the hinge (42) at the threaded hole, and a hand screw (44) passes through the interior of the positioning hole to the interior of the threaded hole, and an outer wall of the hand screw (44) is provided with an external thread that matches the threaded hole.

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