Exosome extraction device

Abstract

An exosome extraction device, comprising: a base (4); a filter column placement disc (1), wherein the filter column placement disc (1) comprises a placement disc body (11), a plurality of extraction column placement holes (111) are formed in the placement disc body (11), the extraction column placement holes (111) are used for placement of exosome extraction columns (100), and the placement disc body (11) can be rotatably mounted on the base (4) relative to the base (4); an exosome collection disc (2), wherein the exosome collection disc (2) comprises a collection disc body (21), a plurality of collection tube placement holes (211) are formed in the collection disc body (21), the collection tube placement holes (211) are used for placement of exosome collection tubes (200), and the collection disc body (21) is rotatably mounted on the base (4) relative to the base (4); and a collection disc translation mechanism for driving the collection disc body (21) to move to a position below the placement disc body (11) or move away from the position below the placement disc body (11), wherein in the state where the collection disc body (21) is located below the collection disc body (11), the placement disc body (11) and the collection disc body (21) rotate, so that the collection tube placement holes (211) can be aligned with the extraction column placement holes (111) so as to collect exosomes dripping from the exosome extraction columns (100).

Description

Exosome extraction equipment Technical Field

[0001] The present application belongs to the field of medical devices, and in particular relates to an exosome extraction device. Background Art

[0002] Extracellular vesicles are generally divided into three types based on their size and biogenesis: exosomes (30-150 nm), microvesicles (100-1000 nm), and apoptotic bodies (greater than 1000 nm).

[0003] Exosomes are tiny vesicles with a lipid bilayer membrane that are secreted by cells within the body. Most cells secrete exosomes, and they are widely distributed in various body fluids, including serum, plasma, saliva, urine, cerebrospinal fluid, and breast milk. Studying the composition of exosomes requires high-quality extraction. However, due to their small particle size and the presence of various other biomolecules, their isolation and purification are difficult, hindering further research.

[0004] In recent years, researchers have proposed a variety of separation and purification methods based on the physical and chemical properties of exosomes. Common methods include ultracentrifugation, density gradient ultracentrifugation, ultrafiltration, polymer precipitation (PEG), immunomagnetic beads, and kit separation.

[0005] In general, the drawbacks of existing exosome extraction technologies include high reliance on instruments such as ultracentrifuges, low separation efficiency, long processing time, low separation purity, and incomplete exosome vesicles. This has significantly limited the application and research of exosomes in various fields, especially in the field of liquid biopsy.

[0006] Summary of the Invention

[0007] This application aims to propose an exosome extraction device to improve the efficiency of exosome extraction.

[0008] The embodiments of the present application provide an exosome extraction device, comprising:

[0009] base;

[0010] A filter column placement tray, comprising a placement tray body, the placement tray body being provided with a plurality of extraction column placement holes, the plurality of extraction column placement holes being arranged along the circumference of the placement tray body, the extraction column placement holes being used to place exosome extraction columns, the placement tray body being rotatably mounted on the base relative to the base;

[0011] An exosome collection tray, comprising a tray body, the tray body being provided with a plurality of collection tube placement holes, the plurality of collection tube placement holes being arranged along the circumference of the tray body, the collection tube placement holes being used to place exosome collection tubes, the tray body being rotatably mounted on the base relative to the base; and

[0012] The collecting tray translation mechanism is used to drive the collecting tray body to move to the bottom of the placing tray body or move away from the bottom of the placing tray body.

[0013] When the collection tray body is located below the placement tray body, the placement tray body and the collection tray body are rotated so that the collection tube placement hole can be aligned with the extraction column placement hole to collect the exosomes dripping from the exosome extraction column.

[0014] In at least one possible embodiment, the number of the extraction column placement holes and the collection tube placement holes is the same, and the placement plate body and the collection plate body are rotated so that the exosome collection tubes in the multiple collection tube placement holes respectively collect the exosomes dripping from the exosome extraction columns in the multiple extraction column placement holes.

[0015] In at least one possible embodiment, the collection tray translation mechanism includes a guide rail and a sliding seat, the collection tray body can be rotatably connected to the sliding seat, and the sliding seat can be slidably connected to the base through the guide rail, and the extension direction of the guide rail is parallel to the horizontal line connecting the central axis of the placement tray body and the central axis of the collection tray body.

[0016] In at least one possible embodiment, the collection tray translation mechanism further includes a collection tray translation motor, a translation driving pulley, a translation driven pulley, and a translation synchronous belt.

[0017] The collection tray translation motor is connected to the base, the translation driving pulley is connected to the output shaft of the collection tray translation motor, the translation driven pulley is rotatably connected to the base, the translation synchronous belt is sleeved on the translation driving pulley and the translation driven pulley, the sliding seat is connected to the translation synchronous belt, and the translation synchronous belt can drive the sliding seat to slide along the guide rail, so that the collection tray body moves to the bottom of the placement tray body or moves away from the bottom of the placement tray body.

[0018] In at least one possible embodiment, the filter column placement tray includes a waste liquid collection box, which is located below the placement tray body, and the multiple extraction column placement holes are all located within the vertical projection range of the waste liquid collection box.

[0019] In at least one possible embodiment, the waste liquid collection box is provided with a notch, so that the collection tray body and the waste liquid collection box can partially overlap in a vertical direction.

[0020] In at least one possible embodiment, the exosome extraction device further includes a liquid adding mechanism.

[0021] The liquid adding mechanism includes a plurality of liquid adding needles, each of which is connected to an independent liquid channel. The liquid adding needles are located above the extraction column placement holes, and the number of the liquid adding needles is less than the number of the extraction column placement holes.

[0022] In at least one possible embodiment, the filter column placement plate further includes a placement plate motor, a placement plate fixing frame and a placement plate transmission mechanism, the placement plate fixing frame is connected to the base, the placement plate motor is fixedly connected to the placement plate fixing frame, and the output end of the placement plate motor is connected to the placement plate body through the placement plate transmission mechanism for driving the placement plate body to rotate.

[0023] In at least one possible embodiment, the placement disk drive mechanism includes two placement disk drive pulleys and a placement disk drive synchronous belt, one of the placement disk drive pulleys is connected to the output end of the placement disk motor, and the other placement disk drive pulley is connected to the placement disk body, and the placement disk drive synchronous belt is sleeved on the two placement disk drive pulleys.

[0024] In at least one possible embodiment, the exosome collection tray further includes a collection tray rotating motor, which is fixedly connected to the sliding seat, and an output end of the collection tray rotating motor is connected to the collection tray body through a collection tray transmission mechanism, for driving the collection tray body to rotate.

[0025] By adopting the above technical solution, the exosome extraction device can extract exosomes from samples in batches with high efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] FIG1 shows a schematic structural diagram of an exosome extraction device according to an embodiment of the present application.

[0027] FIG2 shows a schematic structural diagram of an exosome extraction device according to an embodiment of the present application from another angle.

[0028] FIG3 shows a schematic structural diagram of a filter column placement plate of an exosome extraction device according to an embodiment of the present application.

[0029] FIG4 shows a schematic structural diagram of a filter column placement plate of an exosome extraction device according to an embodiment of the present application from another angle.

[0030] FIG5 shows a schematic structural diagram of an exosome collection plate of an exosome extraction device according to an embodiment of the present application.

[0031] FIG6 shows a schematic structural diagram of an exosome collection plate of an exosome extraction device according to an embodiment of the present application from another angle.

[0032] FIG. 7 shows a flow chart of extracting exosomes using the exosome extraction device according to an embodiment of the present application.

[0033] FIG8 shows a flow chart of cleaning an exosome extraction column using the exosome extraction device according to an embodiment of the present application.

[0034] LIST OF REFERENCE NUMERALS 1 Filter column placement tray 11 Placement tray body 111 Extraction column placement hole 12 Placement tray motor 13 Placement tray fixing frame 14 Placement tray transmission mechanism 141 Placement tray transmission pulley 142 Placement tray transmission synchronous belt 15 Waste liquid collection box 151 Drain hole 2 Exosome collection tray 21 Collection tray body 211 Collection tube placement hole 22 Collection tray rotation motor 23 Collection tray translation motor 24 Guide rail 25 Sliding seat 26 Translation driving pulley 27 Translation driven pulley 28 Translation synchronous belt 29 Collection tray transmission mechanism 3 Liquid adding mechanism 31 Liquid adding needle 32 Pump 4 Base 5 Control panel 100 Exosome extraction column 200 Exosome collection tube Z vertical direction DETAILED DESCRIPTION

[0035] In order to more clearly illustrate the above-mentioned purposes, features and advantages of the present application, this section describes in detail the specific embodiments of the present application in conjunction with the accompanying drawings. In addition to the various embodiments described in this section, the present application can also be implemented in other different ways. Without violating the spirit of the present application, those skilled in the art can make corresponding improvements, deformations and substitutions. Therefore, the present application is not limited to the specific embodiments disclosed in this section. The scope of protection of this application shall be based on the claims.

[0036] As shown in Figures 1 to 8, embodiments of the present application provide an exosome extraction device, which includes a filter column placement tray 1, an exosome collection tray 2, a liquid addition mechanism 3, a base 4, and a control board 5. The filter column placement tray 1, the exosome collection tray 2, the liquid addition mechanism 3, and the control board 5 can all be connected to the base 4.

[0037] As shown in FIG. 1 to FIG. 4 , the filter column placement tray 1 includes a placement tray body 11 , a placement tray motor 12 , a placement tray fixing frame 13 , a placement tray transmission mechanism 14 and a waste liquid collection box 15 .

[0038] The tray mounting bracket 13 is connected to the base 4, and the tray body 11 is rotatably connected to the tray mounting bracket 13. The tray body 11 is provided with a plurality of extraction column placement holes 111. The plurality of extraction column placement holes 111 can be evenly arranged along the circumference of the tray body 11. In this embodiment, nine extraction column placement holes 111 can be provided, for example. The extraction column placement holes 111 can be used to place exosome extraction columns 100, and each extraction column placement hole 111 can accommodate one exosome extraction column 100. The exosome extraction column 100 can be a chromatography column.

[0039] The placement tray motor 12 can be fixedly connected to the placement tray mounting frame 13. The output end of the placement tray motor 12 can be connected to the placement tray body 11 via the placement tray transmission mechanism 14, thereby driving the placement tray body 11 to rotate. In this embodiment, the placement tray transmission mechanism 14 can include two placement tray transmission pulleys 141 and a placement tray transmission timing belt 142. One placement tray transmission pulley 141 is connected to the output end of the placement tray motor 12, and the other placement tray transmission pulley 141 is connected to the placement tray body 11. The placement tray transmission timing belt 142 is sleeved around both placement tray transmission pulleys 141. The placement tray motor 12 can drive the placement tray body 11 to rotate. The control panel 5 can control the rotation angle of the placement tray motor 12 through the control system, thereby precisely controlling the rotation angle of the placement tray body 11, so that the extraction column placement hole 111 can be aligned with the liquid addition needle and collection tube placement hole 211 described later after rotation.

[0040] The waste liquid collection box 15 can be fixedly connected to the placement tray bracket 13 and positioned below the placement tray body 11. The multiple extraction column placement holes 111 are all located within the vertical projection of the waste liquid collection box 15. The waste liquid collection box 15 can collect waste liquid dripping from the exosome extraction columns 100. The bottom of the waste liquid collection box 15 can be provided with a drainage hole 151. The drainage hole 151 can be connected to a drainage tube, which can be connected to a drainage pump. The drainage pump can drain the waste liquid collected by the waste liquid collection box 15 into a waste liquid bottle for storage. The drainage pump can use a diaphragm pump, for example.

[0041] As shown in FIG1 , FIG2 , FIG5 and FIG6 , the exosome collection tray 2 includes a collection tray body 21 , a collection tray rotating motor 22 , and a collection tray translation mechanism.

[0042] The collection tray body 21 is provided with a plurality of collection tube placement holes 211 for placing exosome collection tubes 200, each of which can accommodate a single exosome collection tube 200. The plurality of collection tube placement holes 211 can be evenly arranged along the circumference of the collection tray body 21. In this embodiment, for example, nine collection tube placement holes 211 can be provided. The collection tray translation mechanism can drive the collection tray body 21 to move below or away from the collection tray body 11.

[0043] The collection tray translation mechanism may include a collection tray translation motor 23 , a guide rail 24 , a sliding seat 25 , a translation driving pulley 26 , a translation driven pulley 27 and a translation synchronous belt 28 .

[0044] The collection tray body 21 is rotatably connected to the sliding base 25, and the collection tray rotation motor 22 is fixedly connected to the sliding base 25. The collection tray rotation motor 22 is connected to the collection tray body 21 via a collection tray transmission mechanism 29, thereby driving the collection tray body 21 to rotate. In this embodiment, the collection tray transmission mechanism 29 may include a pulley and a timing belt. The collection tray rotation motor 22 drives the collection tray body 21 to rotate. The control panel 5 controls the rotation angle of the collection tray rotation motor 22 through a control system, thereby precisely controlling the rotation angle of the collection tray body 21.

[0045] The guide rail 24 can be connected to the base 4, and the sliding seat 25 can slide along the guide rail 24. The guide rail 24 can extend horizontally. Specifically, the extension direction X of the guide rail 24 can be parallel to the horizontal line connecting the central axis of the placement tray body 11 and the central axis of the collection tray body 21.

[0046] The collection tray translation motor 23 can be mounted on the base 4. The output shaft of the collection tray translation motor 23 is connected to a translation drive pulley 26. A translation driven pulley 27 is rotatably connected to the base 4. A translation timing belt 28 is mounted on the translation drive pulley 26 and the translation driven pulley 27. The sliding seat 25 is connected to the translation timing belt 28. The translation timing belt 28 drives the sliding seat 25 to slide along the guide rail 24, moving the collection tray body 21 toward or away from the placement tray body 11. When the collection tray body 21 is positioned toward the placement tray body 11, the placement tray body 11 and the collection tray body 21 rotate to align the collection tube placement hole 211 with the extraction column placement hole 111, allowing the liquid in the exosome extraction column 100 to drip into the exosome collection tube 200. When the collection tray body 21 is positioned away from the placement tray body 11, the liquid in the exosome extraction column 100 can drip into the waste liquid collection box 15.

[0047] The sidewalls of the waste liquid collection box 15 may have a notch, allowing the collection tray body 21 and the waste liquid collection box 15 to partially overlap in the vertical direction Z. When the sliding seat 25 drives the collection tray body 21 away from the placement tray body 11, liquid dripping from the exosome extraction column can fall into the waste liquid collection box 15. When the sliding seat 25 drives the collection tray body 21 toward the placement tray body 11, by rotating the collection tray body 21 and the placement tray body 11, the collection tube placement hole 211 can be positioned directly below the extraction column placement hole 111, allowing liquid dripping from the exosome extraction column to fall into the exosome collection tube 200.

[0048] As shown in FIG1 and FIG2 , the liquid adding mechanism 3 includes a liquid adding needle 31 , a pump 32 and a valve.

[0049] The adding needle 31 is installed on the needle holder, which can be connected to the placement tray fixing frame 13. The adding needle 31 is located above the placement tray body 11. By rotating the placement tray body 11, the extraction column placement hole 111 can be aligned with the adding needle 31, and liquid is added to the exosome extraction column 100 through the adding needle 31.

[0050] The dosing needle 31 can be connected to the reagent bottle through a pump 32 and a pipeline (not shown), and the pump 32 can pump the reagent in the reagent bottle to the dosing needle 31. The pipeline connecting the pump 32 and the dosing needle 31 and / or the pipeline connecting the pump 32 and the reagent bottle can be provided with a valve such as a one-way valve or a solenoid valve.

[0051] Multiple dosing needles 31 can be provided, with the number of dosing needles 31 being less than the number of extraction column placement holes 111. Each dosing needle 31 connects to an independent liquid channel. Using multiple dosing needles 31 allows for precise dosing of various reagents during the exosome extraction process. For example, four dosing needles 31 can be provided, each used to add a blocking solution, a washing solution, an equilibration solution, and an eluent to the exosome extraction column 100.

[0052] The dosing needles 31 may include a blocking solution dosing needle 311, a cleaning solution dosing needle 312, an equilibration solution dosing needle 313, and an eluent dosing needle 314. In this embodiment, the blocking solution dosing needle 311, the cleaning solution dosing needle 312, the equilibration solution dosing needle 313, and the eluent dosing needle 314 are arranged in sequence, but the present application does not limit the arrangement order of the multiple dosing needles 31.

[0053] The sealing solution is a solution containing a preservative. The main function of the sealing solution is to protect the exosome extraction column 100 and facilitate the long-term storage of the exosome extraction column 100.

[0054] The cleaning solution is composed of a weak base and is mainly used to elute the residual protein in the exosome extraction column 100 .

[0055] The balancing solution includes a phosphate buffer solution with a pH value between 6.5 and 8.0. The balancing solution is mainly used to flush and replace the preservation solution in the exosome extraction column 100 so that the exosome extraction column 100 reaches a filtering state.

[0056] The eluent includes a phosphate buffer having a pH value between 7.0 and 7.4, and the eluent can simulate the pH environment of the human body. The main function of the eluent is to elute the sample through the exosome extraction column 100 and preserve the integrity of the proteins in the sample.

[0057] The samples for extracting exosomes can be body fluids such as plasma, serum, urine, cerebrospinal fluid, milk, or supernatant of exogenously incubated cells.

[0058] The pump 32 may include a diaphragm pump and a plunger pump. Optionally, the balancing solution and the eluent may be pumped using a plunger pump, while the cleaning solution and the blocking solution may be pumped using a diaphragm pump. The pumping accuracy of the plunger pump is better than that of the diaphragm pump.

[0059] An example of a process for extracting exosomes is described with reference to FIG7 , however, this is merely exemplary and not restrictive.

[0060] ( S11 ) Open the upper cover and the lower cover of the exosome extraction column 100 .

[0061] (S12) The solution is allowed to stand for 3 minutes and the solution is dripped into the waste liquid collection box 15.

[0062] (S13) Then, 2.5 ml of balancing solution is added, and the mixture is allowed to stand for 3 minutes, and then dripped into the waste liquid collection box 15. Step S13 is repeated (circulated) 8 times, that is, a total of 20 ml of balancing solution is added.

[0063] ( S14 ) For example, 0.5 to 1.5 ml of sample is added manually, left to stand for 3 minutes, and then dripped into the waste liquid collection box 15 .

[0064] (S15) Add 2.5 ml of eluent, let it stand for 3 minutes, and drip it into the waste liquid collection box 15.

[0065] (S16) Add 1.5 ml of eluent, let it stand for 3 minutes, and then drip it into the waste liquid collection box 15.

[0066] (S17) 1 ml of eluent is added, the collection plate body 21 is brought close to the placement plate body 11, the extraction column placement hole 111 is aligned with the collection tube placement hole 211, and the solution is dripped into the exosome collection tube 200 for 2 minutes to collect 1 ml of exosomes.

[0067] ( S18 ) For example, 1.5 ml of eluent is manually added to cover the upper and lower covers of the exosome extraction column 100 .

[0068] The exosome extraction column also needs to be cleaned. An example of a process for cleaning the exosome extraction column is described with reference to FIG8 . However, this is merely illustrative and not restrictive.

[0069] ( S21 ) The upper cover and the lower cover of the exosome extraction column 100 are opened.

[0070] (S22) The solution is allowed to stand for 3 minutes and the solution is dripped into the waste liquid collection box 15.

[0071] (S23) Add 2.5 ml of cleaning solution, let it stand for 3 minutes, and then drip it into the waste liquid collection box 15. Here, step S23 is repeated (circulated) 4 times, that is, a total of 10 ml of cleaning solution is added.

[0072] (S24) Then, 2.5 ml of balancing solution is added, and the mixture is allowed to stand for 3 minutes before dripping into the waste liquid collection box 15. Step S24 is repeated (circulated) 8 times, i.e., a total of 20 ml of balancing solution is added.

[0073] (S25) Add 2.5 ml of blocking solution, let it stand for 3 minutes, and then drip it into the waste liquid collection box 15. Here, step S25 is repeated (circulated) 3 times, that is, a total of 7.5 ml of blocking solution is added.

[0074] ( S26 ) 1.5 ml of blocking solution was added to cover the upper and lower caps of the exosome extraction column 100 .

[0075] It is understood that while the exosome extraction column 100 is placed at any position in the tray body 11, the exosome extraction columns 100 at other positions can be added with liquid, which is equivalent to fully utilizing the waiting time for the static dripping. The exosome extraction device of the present application can be used to extract exosomes from samples in batches with high efficiency.

[0076] The present application is not limited to the above embodiments, and those skilled in the art can make various modifications to the above embodiments under the guidance of the present application without departing from the scope of the present application.

[0077] (1) In the above embodiment, the extension direction of the guide rail 24 is parallel to the horizontal line connecting the central axis of the placement tray body 11 and the central axis of the collection tray body 21. However, the present application is not limited to this. The extension direction of the guide rail can also be perpendicular to the extension direction of the guide rail in the above embodiment.

[0078] (2) In the above embodiment, the number of the extraction column placement holes 111 and the collection tube placement holes 211 are both 9, which is exemplary and not restrictive.

[0079] (3) The placement tray transmission mechanism and the collection tray transmission mechanism are not limited to the form of belts and pulleys, and other components or mechanisms including gears, chains, etc. can also be used.

[0080] (4) The collection tray translation motor 23, the guide rail 24 and the sliding seat 25 can constitute a collection tray translation mechanism, but the present application is not limited thereto. The collection tray translation mechanism can also use other components or mechanisms including gears, racks, linear motors, screws, etc.

[0081] (5) In the above embodiment, the filter column tray 1 and the exosome collection tray 2 are both mounted on a base. It is understood that the base may be a single integral component or may be a plurality of separate components. For example, the base or base portion to which the filter column tray 1 is mounted may be separate from the base or base portion to which the exosome collection tray 2 is mounted.

[0082] It should be understood that at least some aspects or features of the above-mentioned embodiments, examples or examples may be appropriately combined.

[0083] It is understood that in this application, when the number of parts or components is not specifically limited, the number may be one or more, and the term "plurality" herein refers to two or more. Where the number of parts or components is shown in the drawings and / or described in the specification as a specific number, such as two, three, or four, the specific number is generally illustrative and not restrictive, and may be understood as a plurality, i.e., two or more. However, this does not mean that this application excludes the case of one.

[0084] In this application, unless otherwise clearly stated or limited, terms such as "install", "assemble", "connect", "connect", "couple", "link", "abut", "connect", "interconnect", "communicate", "conduct", "fix", "fasten", etc. should be understood in a broad sense, for example, they can be direct or indirect. For example, with respect to connection, it can be a fixed connection, a detachable connection, or an integrated connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly stated or limited. For example, with respect to connectivity / conduction, it can be direct connectivity / conduction or indirect connectivity / conduction through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to the specific circumstances.

[0085] In the present application, unless otherwise clearly stated or limited, a component provided on / installed on / located on / accommodated on / placed in, within, inside, etc. another component may be any of the following two situations: a part or most of the one component is located in the other component; and the one component is completely accommodated in the other component.

[0086] While the present application has been described in detail using the above-described embodiments, it will be apparent to those skilled in the art that the present application is not limited to the embodiments described in this specification. The present application can be modified and implemented as modified embodiments without departing from the subject matter and scope of the present application as defined by the claims. Therefore, the descriptions in this specification are for illustrative purposes only and do not have any limiting meaning with respect to the present application.

Claims

1. An exosome extraction device, characterized in that, Comprising: A base (4); A filter column placement tray (1), the filter column placement tray (1) includes a placement tray body (11), the placement tray body (11) is provided with a plurality of extraction column placement holes (111), the plurality of extraction column placement holes (111) are arranged along the circumferential direction of the placement tray body (11), the extraction column placement holes (111) are used for placing exosome extraction columns (100), and the placement tray body (11) is rotatably mounted on the base (4) relative to the base (4); An exosome collection tray (2), the exosome collection tray (2) includes a collection tray body (21), the collection tray body (21) is provided with a plurality of collection tube placement holes (211), the plurality of collection tube placement holes (211) are arranged along the circumferential direction of the collection tray body (21), the collection tube placement holes (211) are used for placing exosome collection tubes (200), and the collection tray body (21) is rotatably mounted on the base (4) relative to the base (4); And A collection tray translation mechanism for driving the collection tray body (21) to move below the placement tray body (11) or to move away from below the placement tray body (11), In a state where the collection tray body (21) is located below the placement tray body (11), by rotating the placement tray body (11) and the collection tray body (21), the collection tube placement holes (211) can be aligned with the extraction column placement holes (111) to collect exosomes dripping from the exosome extraction columns (100).

2. The exosome extraction device according to claim 1, wherein The number of the extraction column placement holes (111) is the same as that of the collection tube placement holes (211). By rotating the placement tray body (11) and the collection tray body (21), the exosome collection tubes (200) in the plurality of collection tube placement holes (211) respectively collect the exosomes dripping from the exosome extraction columns (100) in the plurality of extraction column placement holes (111).

3. The exosome extraction device according to claim 1, wherein, The collection tray translation mechanism includes a guide rail (24) and a sliding seat (25). The collection tray body (21) is rotatably connected to the sliding seat (25). The sliding seat (25) is slidably connected to the base (4) through the guide rail (24). The extending direction (X) of the guide rail (24) is parallel to the horizontal connection line of the central axis of the placement tray body (11) and the central axis of the collection tray body (21).

4. The exosome extraction device according to claim 3, wherein, The collection tray translation mechanism further includes a collection tray translation motor (23), a translation driving pulley (26), a translation driven pulley (27), and a translation synchronous belt (28). The collecting tray translation motor (23) is connected to the base (4). The translation driving pulley (26) is connected to the output shaft of the collecting tray translation motor (23). The translation driven pulley (27) is rotatably connected to the base (4). The translation synchronous belt (28) is sleeved on the translation driving pulley (26) and the translation driven pulley (27). The sliding seat (25) is connected to the translation synchronous belt (28). The translation synchronous belt (28) can drive the sliding seat (25) to slide along the guide rail (24), so that the collecting tray body (21) moves below the placement tray body (11) or moves away from below the placement tray body (11).

5. The exosome extraction device according to claim 1, wherein The filter column placement tray (1) includes a waste liquid collection box (15). The waste liquid collection box (15) is located below the placement tray body (11). The plurality of extraction column placement holes (111) are all within the vertical projection range of the waste liquid collection box (15).

6. The exosome extraction device according to claim 5, wherein, The waste liquid collection box (15) is provided with a notch, so that the collecting tray body (21) and the waste liquid collection box (15) can partially overlap in the vertical direction (Z).

7. The exosome extraction device according to claim 1, characterized in that, The exosome extraction device further includes a liquid adding mechanism (3). The liquid adding mechanism (3) includes a plurality of liquid adding needles (31). Each liquid adding needle (31) communicates with an independent liquid channel. The liquid adding needles (31) are located above the extraction column placement holes (111). The number of liquid adding needles (31) is less than the number of extraction column placement holes (111).

8. The exosome extraction device according to claim 1, characterized in that, The filter column placement tray (1) further includes a placement tray motor (12), a placement tray fixing frame (13), and a placement tray transmission mechanism (14). The placement tray fixing frame (13) is connected to the base (4). The placement tray motor (12) is fixedly connected to the placement tray fixing frame (13). The output end of the placement tray motor (12) is connected to the placement tray body (11) through the placement tray transmission mechanism (14) for driving the placement tray body (11) to rotate.

9. The exosome extraction device according to claim 8, characterized in that, The placement tray transmission mechanism (14) includes two placement tray transmission pulleys (141) and a placement tray transmission synchronous belt (142). One placement tray transmission pulley (141) is connected to the output end of the placement tray motor (12). The other placement tray transmission pulley (141) is connected to the placement tray body (11). The placement tray transmission synchronous belt (142) is sleeved on the two placement tray transmission pulleys (141).

10. The exosome extraction device according to claim 3, characterized in that, The exosome collection tray (2) further includes a collection tray rotation motor (22). The collection tray rotation motor (22) is fixedly connected to the sliding seat (25). The output end of the collection tray rotation motor (22) is connected to the collection tray body (21) through a collection tray transmission mechanism (29) for driving the collection tray body (21) to rotate.

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