Cell lysis system

Abstract

The utility model discloses a cell lysis system, which is provided with a coil pipe frame and a carrier for bearing a coil pipe, a support frame provides a bearing surface for winding the coil pipe, an accommodating area for accommodating the coil pipe is formed between the support frame and a main body, and meanwhile, the support frame is rotationally connected with the main body, so that when the coil pipe is mounted, the support frame can be rotated, and the coil pipe can be mounted on the main body. The coil pipes are evenly wound on the supporting frame, even distribution of the coil pipes on the supporting frame is achieved, the possibility that the coil pipes extrude one another is reduced, in addition, the supporting frame can rotate, the winding difficulty of the coil pipes is reduced, the whole coil pipes do not need to be turned over, and when the coil pipes are installed, only one ends of the coil pipes need to be fixedly connected with the supporting frame, and the other ends of the coil pipes are free ends. The winding device is convenient to replace, high in efficiency and uniform in winding.

Description

Technical Field

[0001] The utility model relates to the technical field of cell lysis, in particular to a cell lysis system. Background Art

[0002] With the rapid development of the field of molecular biology in recent years, especially the gradual large-scale commercialization in the biopharmaceutical industry, cells (such as animals, plants, and microorganisms) as one of the key research objects in the field of molecular biology have shown rapid and quantitative research in the directions of genetic engineering and cell engineering. Among them, the technical demand for cell lysis to obtain intracellular substances has increased sharply.

[0003] In particular, taking Escherichia coli, a model organism widely used in molecular biology research at present, as an example, its expression product plasmid is used as a genetic engineering expression vector and a gene editing tool. Currently, high-density fermentation of Escherichia coli is usually used in production, that is, alkaline lysis of the bacterial cells is carried out to release the plasmid. The specific process is well known in the art and will not be elaborated here. However, due to the complex and variable and environment-sensitive biological characteristics of Escherichia coli and the plasmid, the difficulty and requirements of the bacterial cell lysis process have been increased. Especially, the performance of the lysis solution, the mixing time and effect of the feed liquid can directly affect the lysis efficiency and effect, as well as the activity and purity of the plasmid, and then have an adverse impact on the subsequent purification process and process difficulty, thereby reducing the stability of the plasmid production process and the plasmid yield.

[0004] According to the prior art disclosed in Patent EP1628749B1: During the cell lysis process, in order to ensure sufficient cell lysis and lysis effect, the cell suspension is usually mixed with the lysis solution and then passed through a coiled pipe with a determined length to ensure the cell lysis effect. Therefore, the length of the coiled pipe can adjust the duration of the lysis process (i.e., the length of time), that is, different lysis durations correspond to coiled pipes of different lengths. When different lysis processes are carried out to meet different lysis requirements (such as the amount or ratio of the lysate, etc.), generally, the coiled pipe needs to be replaced, which results in the need to rewind the coiled pipe every time the lysis process is changed.

[0005] Currently, it mainly relies on manual winding of the coiled pipe, which is time-consuming and laborious, greatly reducing the replacement efficiency; and due to human error, it is easy to cause uneven coiling of the coiled pipe or even mutual overlapping; especially when the material of the coiled pipe is flexible, the overlapping of the coiled pipes affects the flow rate of the feed liquid in the coiled pipe, resulting in different outlet flow rates of the coiled pipe and the neutralizing liquid, and causing the mixing process in the second static mixer to be out of sync, affecting the mixing effect; moreover, the too long coiled pipe also leads to time-consuming and laborious coiling.

[0006] Therefore, it is necessary to design a cell lysis system to solve the problems of inconvenient replacement, low efficiency, uneven winding and even mutual overlapping of the existing coiled pipes. Summary of the Utility Model

[0007] Aiming at the deficiencies of the prior art, the purpose of the present utility model is to provide a cell lysis system, which solves the problems of inconvenient replacement, low efficiency, uneven winding and even overlapping of existing coiled pipes.

[0008] In order to achieve the above purpose, the present utility model adopts the following technical solutions:

[0009] A cell lysis system includes a bacterial liquid supply device, a lysis liquid supply device, a first mixing device, a second mixing device, and a neutralizing liquid supply device. The bacterial liquid supply device and the lysis liquid supply device are both connected to the first mixing device. The first mixing device further has an outlet end for communicating with the inlet of the coiled pipe. The neutralizing liquid supply device is connected to the second mixing device. The second mixing device further has an inlet end for communicating with the outlet of the coiled pipe;

[0010] The cell lysis system further includes a coiled pipe rack, and the coiled pipe rack includes:

[0011] A main body;

[0012] A support frame, rotatably connected to the main body, and an accommodating area is formed between the support frame and the main body;

[0013] The support frame has a bearing surface for carrying the coiled pipe, so that when the support frame rotates relative to the main body, the coiled pipe surrounds and covers the bearing surface and at least partially fills the accommodating area.

[0014] The cell lysis system of the present utility model uses a coil rack as a carrier for the coil. Among them, the support frame provides a bearing surface for the coil to wind around. The support frame and the main body together form an accommodating area for the coil. At the same time, the support frame is rotatably connected to the main body. When installing the coil, the support frame can be rotated so that the coil is evenly wound around the support frame, realizing the uniform distribution of the coil on the support frame, reducing the possibility of the coils squeezing each other. In addition, the rotatability of the support frame also reduces the difficulty of winding the coil. When installing the coil, only one end of the coil needs to be fixed on the support frame (the fixing method can be directly connected to the communicating part of the support frame or assisted by mechanical components, and the fixing method can be detachable or non-detachable, as long as it is any one of the fixing methods well-known to those skilled in the art that can realize the fixation between the coil and the support frame). The other end is a free end, which winds around the bearing surface as the support frame rotates. Finally, the free end of the coil is fixed so that the coil is stably installed in the accommodating area, ensuring that the coil will not move randomly during use. Such a setting makes the coils not overlap each other, avoiding affecting the flow rate of the liquid material filled in the coil during the operation of the subsequent cell lysis system, which is beneficial to ensuring the stable flow rate of the liquid material and its adaptation to the liquid discharge flow rate of the neutralizing liquid, beneficial to improving the synchronization of the process of the two entering the second mixing device and mixing, with a better mixing effect. At the same time, the coil winding process is convenient to replace, efficient, and evenly wound.

[0015] Preferably, at least one positioning shaft is provided between the main body and the support frame. The main body is provided with a positioning part to limit the position of the positioning shaft. The support frame rotates around the positioning shaft to form a rotation center.

[0016] The positioning shaft has a first inner cavity that penetrates through both ends along its axis; the positioning shaft has a first communicating part for connecting with the coil so that the coil communicates with the first inner cavity of the positioning shaft.

[0017] The positioning part on the main body can limit the position of the positioning shaft, ensuring the stable connection between the support frame connected to it and the main body and enabling smooth rotation; the functions of the positioning shaft include: on the one hand, as the rotation center of the support frame, the support frame rotates around the axis of the positioning shaft, making the process of winding the coil more labor-saving; on the other hand, since the positioning shaft has a first communicating part, the positioning shaft can also serve as the communicating part of the coil to realize the connection between the coil and the first mixing device or the second mixing device. Since the positioning shaft is the rotation center of the support frame, when the support frame rotates, the distance between the positioning shaft and the bearing surface on the support frame basically does not change. Therefore, if the positioning shaft is connected and fixed to the fixed end of the coil, when the support frame rotates, the fixed end of the coil is relatively stationary with respect to the support frame, and will not cause excessive pulling on the positioning shaft and the fixed end of the coil, resulting in damage to the coil.

[0018] Preferably, after the bearing surface is covered by the coil pipe, a winding center is formed, and an area covered by the coil pipe is formed;

[0019] The winding center is concentrically arranged with the positioning shaft; alternatively, the winding center is eccentrically arranged with the positioning shaft.

[0020] The covered area is the projection area of the coil pipe on the axial direction after the coil pipe is wound on the support frame. The winding center is actually the center of the projection area. In some embodiments, the bearing surface is circumferentially distributed around the positioning shaft, and the formed winding center is also concentric with the positioning shaft. After the coil pipe is wound on the support frame, it is evenly distributed around the positioning shaft, and the weight is relatively balanced; in other embodiments, the bearing surface is eccentrically arranged relative to the positioning shaft, and the formed winding center is also eccentrically arranged relative to the positioning shaft. After the coil pipe is wound on the support frame, it is also eccentrically arranged relative to the positioning shaft, so that the coil pipe is naturally located in the lower half of the accommodation space when placed in the accommodation space, which is beneficial to protecting the coil pipe.

[0021] Preferably, the first communication part is located within the covered area, that is, the positioning shaft is located within the covered area, and the connection part between the coil pipe and the positioning shaft is also located within the covered area. After the coil pipe is wound on the support frame, the first communication part can be covered, thereby avoiding accidental damage to the connection part between the coil pipe and the positioning shaft and ensuring the connection stability between the coil pipe and the positioning shaft and other devices.

[0022] Preferably, at least one communication pipe is further provided on the main body. The communication pipe penetrates through the main body along its axial direction. The communication pipe has a second inner cavity penetrating through both ends along its axis. The communication pipe further has a second communication part located in the accommodation space for connecting with the coil pipe, so that the coil pipe is communicated with the second inner cavity.

[0023] The positioning shaft and the communication pipe are not the same pipe. The coil pipe may not be communicated with the positioning shaft, but is communicated with the first mixing device or the second mixing device through the communication pipe. The installation position of the communication pipe is more flexible. The user can set the position of the communication pipe according to their own needs, and the installation is more convenient and applicable to different first mixing devices and second mixing devices.

[0024] Preferably, after the coil pipe is wound on the support frame, the communication pipe is located within the covered area, or the communication pipe is located outside the covered area.

[0025] In some embodiments, when the coil pipe is communicated with the communication pipe, the communication pipe can be located within the covered area, and its connection with the coil pipe is protected by being wound on the support frame, ensuring the connection stability between the coil pipe and the positioning shaft and other devices; in other embodiments, it is not easy to connect the coil pipe with the communication pipe located within the covered area. For example, after the winding of the coil pipe is completed, the free end of the coil pipe is not easy to be placed within the covered area, and it is more convenient to communicate with the communication pipe located outside the covered area without the need to adjust the already wound coil pipe.

[0026] Preferably, when the winding center is concentric with the positioning shaft,

[0027] The positioning shaft located at one axial end of the support frame has the first communication part for connecting with one end of the coil pipe, and the first communication part is located within the coverage area;

[0028] A communication pipe is provided on the main body located at the other axial end of the support frame. The communication pipe passes through the main body along its axial direction. The communication pipe has a second inner cavity penetrating through both ends along its axis and a second communication part for connecting with the other end of the coil pipe. The communication pipe and the second communication part are located outside the coverage area.

[0029] Preferably, the positioning part is a positioning hole, and the positioning shaft passes through the positioning hole;

[0030] The positioning shaft is fixedly connected with the positioning hole, and the support frame rotates circumferentially around the positioning shaft;

[0031] Or, the positioning shaft is rotatably connected with the positioning hole, and the support frame is fixedly connected with the positioning shaft.

[0032] With the above structure, on the one hand, whether the support frame rotates around the positioning shaft or the support frame and the positioning shaft rotate together in the positioning hole, the positioning hole can play a role in limiting the installation of the positioning shaft and the support frame. On the other hand, one end of the positioning shaft is located within the accommodation space of the main body, and the other end of the positioning shaft passes through the positioning hole and is located outside the main body and not within the coverage area of the coil pipe. After the coil pipe is wound, the end part of the positioning shaft that exposes the main body and is not within the coverage area of the coil pipe can be conveniently connected with an external first mixing device or second mixing device;

[0033] In some specific embodiments, the coil pipe is in a communication state with the positioning shaft during use. The positioning shaft and the positioning hole can be rotatably connected, and the positioning shaft and the support frame are fixedly connected. Thus, during the process of the coil pipe winding around the support frame, the connection part between the coil pipe and the positioning shaft rotates together with the support frame, without circumferentially pulling the coil pipe or the positioning shaft, and the rotation is smoother, avoiding the twisting of the fixed end of the coil pipe.

[0034] In some other embodiments, the positioning shaft and the positioning hole are fixedly connected, the positioning shaft and the support frame are rotatably connected, and the coiled pipe is in a communicating state with the positioning shaft during use. Then, during the winding process of the coiled pipe, there is axial pre-fixation between the fixed end of the coiled pipe and the positioning shaft (i.e., the fixed end of the coiled pipe and the positioning shaft are detachably fixed). At this time, the distance between the fixed end of the coiled pipe and the bearing surface on the support frame remains unchanged. As the support frame rotates relative to the positioning shaft, the free end of the coiled pipe will be evenly wound around the support frame, and the fixed end of the coiled pipe is connected to the first communicating part of the positioning shaft and rotates relative to the positioning shaft following the support frame. If the fixed end of the coiled pipe is distorted during the rotation, after the coiled pipe is wound, the positioning shaft or the support frame with the wound coiled pipe can be removed, the fixed end of the coiled pipe can be restored, and then it can be completely fixed to the first communicating part of the positioning shaft again to achieve the communication between the positioning shaft and the coiled pipe; or, during the winding of the coiled pipe, the fixed end of the coiled pipe is not connected to the positioning shaft but is pre-fixed to other fixed parts or fixed components on the support frame, and then after the coiled pipe is completely wound, the fixed end is connected to the positioning shaft to achieve the communication between the positioning shaft and the coiled pipe. The other end of the positioning shaft is communicated with an external first mixing device or second mixing device. Therefore, generally, the other end of the positioning shaft is exposed outside the main body to facilitate the connection with the first mixing device or the second mixing device.

[0035] Preferably, when the positioning shaft is rotatably connected to the support frame, a wear-resistant member is provided between the positioning shaft and the support frame, so that the wear-resistant member can reduce the rotational friction between the positioning shaft and the support frame, extend the service life of the positioning shaft and the support frame, and at the same time can also protect the first inner cavity in the positioning shaft to avoid leakage at the positioning shaft where the coiled pipe is communicated with the first mixing device or the second mixing device.

[0036] Preferably, the positioning shaft, the winding center, and the rotation center are all horizontally arranged. On the one hand, during the process of winding the coiled pipe around the support frame, the support frame is horizontally arranged, which is convenient for the coiled pipe to be evenly wound, and it is more labor-saving for the operator to rotate the support frame; on the other hand, the wound coiled pipe is also in a horizontal state during use, and the coiled pipe is evenly stressed at each part, avoiding the coiled pipe being stacked and deformed due to uneven stress at each part, thereby affecting the flow rate of the liquid material.

[0037] Preferably, among all the positioning shafts, the sizes of the first inner cavity are at least two kinds, and positioning shafts with different-sized first inner cavities can be correspondingly connected to coiled pipes with different inner diameters, so that the coiled pipe rack is suitable for coiled pipes with different inner diameters.

[0038] Preferably, the support frame includes at least one support plate and at least two support rods extending axially along the positioning shaft. A plurality of the support rods are connected to the support plate, and the bearing surface is located on the surface of the support rods and the inner side surface where the support plate is connected to the support rods. The support frame is in an assembled form, and the processing process is simple and feasible; the coiled pipe can be evenly wound around the support rods, and the axial two ends of the wound coiled pipe can abut against the inner side surface of the support plate to axially limit the coiled pipe and further stabilize the coiled pipe.

[0039] In one embodiment, a plurality of the support rods are circumferentially and evenly distributed around the axis of the positioning shaft, that is, the bearing surface is circumferentially and evenly distributed around the positioning shaft, and the coiled pipe is wound around the positioning shaft. When the support frame is rotated, it is more stable and labor-saving;

[0040] In another embodiment, a plurality of the support rods are eccentrically arranged on the support plate relative to the axis of the positioning shaft, that is, the bearing surface is eccentrically arranged relative to the positioning shaft, and the coiled pipe is also eccentrically arranged relative to the positioning shaft after being wound on the support frame. When the coiled pipe is placed in the main body, it naturally lies in the lower half of the accommodating space under the action of gravity, which is beneficial to protecting the coiled pipe.

[0041] Regardless of whether the support rods are circumferentially and evenly distributed or eccentrically arranged relative to the positioning shaft, the bearing surface formed by a plurality of support rods is axisymmetrically distributed, ensuring the uniformity of the position of the coiled pipe on the support frame, avoiding partial indentation and causing folding or twisting of the pipe body, etc. And a plurality of support rods provide a suitable winding space for the coiled pipe, avoiding too small a winding radius of the coiled pipe and causing damage to the turning position, affecting the internal flow channel of the coiled pipe.

[0042] Preferably, a circumferential gap is provided between a plurality of the support rods. On the one hand, it can reduce the weight of the support frame. On the other hand, in an embodiment where the positioning shaft is communicated with the coiled pipe, the circumferential gap can also allow the fixed end of the coiled pipe to pass through between the support rods and be communicated with the positioning shaft.

[0043] Preferably, the support rods and the support plate are detachably fixed and can be fixed after being moved to a set position, and the size of the enclosure formed by the support rods can be determined according to needs; for a coiled pipe with a longer length, the support rods can be relatively far away from each other, so that the winding radius of the coiled pipe is larger and it can be wound only in one layer, avoiding the coiled pipes being stacked and squeezed against each other when wound in multiple layers, affecting the size of the internal flow channel of the coiled pipe; for a coiled pipe with a shorter length, the support rods can be relatively close to each other, so that the coiled pipe is distributed as much as possible on the surface surrounded by a plurality of support rods, and the two axial sides of the coiled pipe abut against two support plates respectively, avoiding leaving extra space on the support frame and causing the coiled pipe to axially move on the support rods, pulling the connection at both ends of the coiled pipe and affecting the stable connection between the coiled pipe and the positioning shaft or the connecting pipe.

[0044] Preferably, the bearing surface formed on the surface of the support rod is a continuous closed curved surface, so as to avoid sharp protrusions on the surface of the support rod that may damage the coil, and the coil can be more flat when it is wound on the support rod.

[0045] Preferably, the main body includes two vertical plates and a surrounding piece, the two vertical plates are arranged opposite to each other along the axial direction of the support frame, the vertical plates and the surrounding piece form a receiving space with one side open, and as the support frame rotates, the coil is allowed to at least partially fill the receiving space; the vertical plates and the surrounding piece surround the support frame, thereby forming a receiving space to surround the coil and protect the coil from external force damage; the vertical plates are also used to install the positioning shaft and the support frame.

[0046] Preferably, the support frame and / or the main body are provided with a plurality of openings, so that the coil rack is lightweight as a whole and easy to transport. Meanwhile, the light weight of the support frame also makes it easier to rotate the support frame to wind the coil, and is convenient to operate.

[0047] Preferably, the cell lysis system further comprises a control cabinet carrying a software control system, and the coil rack is embedded in the control cabinet, which is conducive to the structural intensiveness of the entire cell lysis system.

[0048] In summary, compared with the prior art, the utility model has at least the following beneficial effects:

[0049] The cell lysis system of the utility model is provided with a coil rack as a carrier for carrying the coil, wherein the support frame provides a bearing surface for winding the coil, and the support frame and the main body together form a accommodating area for accommodating the coil, and at the same time, the support frame is rotatably connected to the main body. When installing the coil, the support frame can be rotated so that the coil is evenly wound on the support frame, so that the coil is evenly distributed on the support frame, and the possibility of the coils squeezing each other is reduced. In addition, the rotatable support frame also reduces the difficulty of winding the coil. When installing the coil, only one end of the coil needs to be fixed on the support frame (the fixing method can be directly connected to the connecting part of the support frame, or assisted by mechanical parts, and the fixing method can be detachable or non-detachable). The coil is detachable, and any method known to those skilled in the art that can achieve the fixing between the coil and the support frame is sufficient), and the other end is a free end, which is wound on the bearing surface as the support frame rotates, and finally the free end of the coil is fixed, so that the coil is stably installed in the accommodating area, ensuring that the coil will not move randomly during use. This arrangement prevents the coils from overlapping each other, avoiding affecting the flow rate of the feed liquid filled into the coil in the working state of the subsequent cell lysis system, thereby ensuring that the flow rate of the feed liquid is stable and compatible with the flow rate of the neutralization liquid, and is conducive to improving the synchronization of the process of the two entering the second mixing device and mixing, with a good mixing effect. At the same time, the winding process is convenient to replace, efficient, and uniform. Brief Description of the Figures

[0050] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for use in the description of the specific embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0051] Figure 1 It is a schematic framework diagram of the cell lysis system according to an embodiment of the present invention.

[0052] Figure 2 It is a schematic structural diagram of the coil rack according to an embodiment of the present invention.

[0053] Figure 3 It is an axial sectional view of the coil rack according to an embodiment of the present invention.

[0054] Figure 4 It is a sectional view of the coil rack from another angle according to an embodiment of the present invention, showing that the positioning shaft is concentrically arranged with the winding center.

[0055] Figure 5 It is a sectional view of the coil rack according to another embodiment of the present invention, showing that the positioning shaft is eccentrically arranged with the winding center, and the positioning shaft is located in the covering area.

[0056] Figure 6 It is a sectional view of the coil rack according to another embodiment of the present invention, showing that the positioning shaft is eccentrically arranged with the winding center, and the positioning shaft is located outside the covering area.

[0057] Explanation of reference numerals

[0058] 1. Bacterial liquid supply device; 2. Lysis liquid supply device; 3. First mixing device; 4. Second mixing device; 5. Neutralizing liquid supply device;

[0059] 10. Coil; 11. Fixed end; 12. Free end;

[0060] 20. Main body; 21. Positioning hole; 22. Winding center; 23. Covering area; 24. Connecting pipe; 241. Second inner cavity; 242. Second connecting part; 25. Vertical plate; 26. Enclosing member;

[0061] 30. Support frame; 31. Bearing surface; 32. Positioning shaft; 321. First inner cavity; 322. First connecting part; 323. Wear-resistant part; 34. Support plate; 35. Support rod; 351. Circumferential gap; 36. Opening; 37. Rotation center;

[0062] 40. Control cabinet. Detailed embodiments

[0063] The technical solution of the present utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the present utility model, rather than all embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative work shall fall within the protection scope of the present utility model.

[0064] In the description of the present utility model, it should be noted that the orientation or positional relationship indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as a limitation of the present utility model. In addition, the terms "first", "second", "third" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance.

[0065] In the description of the present utility model, it should be noted that unless otherwise clearly specified and defined, the terms "installed", "connected", "connected" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and it can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations.

[0066] The general process of cell lysis is as follows: First, the cell suspension is fully mixed with the lysis solution to lyse the cells and release the substances inside the cells. The substances may include cell debris, cell organelles, and cell products, etc. In the present utility model, the preparation of plasmid by lysing Escherichia coli is taken as an example for illustration. In the alkaline lysis plasmid extraction method for obtaining plasmids from Escherichia coli, the cell suspension is generally called the bacterial solution, and the cells in it are Escherichia coli. The alkaline denaturation extraction of plasmid DNA is based on the differences in denaturation and renaturation between chromosomal DNA and plasmid DNA to achieve separation. Therefore, this method generally includes three steps: 1. Add an alkaline reagent solution (i.e., the lysis solution) to the bacterial solution, and quickly and fully mix the lysis solution and the bacterial solution to form a primary mixture; 2. After the bacterial solution and the lysis solution are fully mixed and reacted, a large amount of plasmid DNA is released; 3. Add an acidic salt solution to neutralize the pH value, fully mix and react to form a final mixture. The final mixture is subjected to subsequent purification treatment to obtain the cell product.

[0067] Based on the above process, as shown in the attached Figure 1As shown in the figure, the cell lysis system of the present embodiment of the utility model includes a bacterial liquid supply device 1, a lysis liquid supply device 2, a first mixing device 3, a second mixing device 4, and a neutralizing liquid supply device 5. The bacterial liquid supply device 1 is used to provide a bacterial liquid containing cells and cell products, the lysis liquid supply device 2 is used to provide a lysis liquid, generally an alkaline reagent solution, and the neutralizing liquid supply device 5 is used to provide a neutralizing liquid, generally an acidic reagent solution. Both the bacterial liquid supply device 1 and the lysis liquid supply device 2 are connected to the first mixing device 3. The bacterial liquid and the lysis liquid are mixed and reacted in the first mixing device 3 to form a primary mixture. The first mixing device 3 also has an outlet end for communicating with the inlet of the coil 10. The primary mixture enters the coil 10 for full reaction to form a transition mixture. The neutralizing liquid supply device 5 is connected to the second mixing device 4. The second mixing device 4 also has an inlet end for communicating with the outlet of the coil 10. The transition mixture and the neutralizing liquid enter the second mixing device 4 for full mixing to form a final mixture.

[0068] It should be noted that the coil 10 is detachably connected to the first mixing device 3 and the second mixing device 4. The coil 10 may not be included when the cell lysis system is sold at the factory. During the use of the cell lysis system, the user can use the coil 10 prepared by himself. Generally, the above-mentioned various components need to be assembled on-site during use, and the winding of the coil 10 is very troublesome. Therefore, as shown in the appendix Figure 2 As shown in the figure, the cell lysis system of the present embodiment further includes a coil rack, which includes: a main body 20; a support frame 30 rotatably connected to the main body 20 and forming an accommodating area with the main body 20; the support frame 30 has a bearing surface 31 for carrying the coil 10, so that when the support frame 30 rotates relative to the main body 20, the coil 10 surrounds and covers the bearing surface 31 and at least partially fills the accommodating area.

[0069] Specifically, the support frame 30 is provided for the coil pipe 10 to wind around. As a carrier for carrying the coil pipe 10, the support frame 30 can be located inside, outside, or on the side of the main body 20, and an accommodation area for accommodating the coil pipe 10 is formed between the support frame 30 and the main body 20, which is jointly formed by the support frame 30 and the main body 20. The support frame 30 is rotatably connected to the main body 20. When installing the coil pipe 10, first place the coil pipe 10 on the bearing surface 31 of the support frame 30. The bearing surface 31 includes all parts of the support frame 30 that come into contact with the coil pipe 10. The support frame 30 can be rotated, and the coil pipe 10 is evenly wound around the support frame 30. At the same time, the coil pipe 10 is also evenly and orderly filled in the accommodation area, so as to realize the uniform and orderly storage of the coil pipe 10, which is time-saving and labor-saving. Finally, fix the free end of the coil pipe 10 so that the coil pipe 10 is stably installed in the accommodation area, ensuring that the coil pipe 10 will not move randomly during use. Such a setting makes the coil pipes 10 not overlap each other and affect the flow rate of the liquid material, avoiding affecting the flow rate of the liquid material filled into the coil pipe 10 under the working state of the subsequent cell lysis system, ensuring that the flow rate of the liquid material is stable and adapted to the liquid discharge flow rate of the neutralizing liquid, and the process of the two entering the second mixing device 4 and mixing is relatively synchronous, and the mixing effect is good.

[0070] It should be noted that the axial direction, circumferential direction, and radial direction in this embodiment are based on the axial direction, circumferential direction, and radial direction of the support frame 30, and the orientation standards of each component in the support frame 30 are the same.

[0071] It should be noted that the free end of the coil pipe 10 refers to the end that can move freely during the winding process of the coil pipe. After the coil pipe is wound, the free end also needs to be fixed.

[0072] At least one positioning shaft 32 is provided between the main body 20 and the support frame 30. The main body 20 is provided with a positioning portion to limit the position of the positioning shaft 32. The support frame 30 rotates around the positioning shaft 32 to form a rotation center 37. In a specific embodiment, only one positioning shaft 32 can be provided between the main body 20 and the support frame 30. The support frame 30 is vertically arranged, and the positioning shaft 32 is arranged at the bottom connection of the main body 20 and the support frame 30; or in another embodiment, only one positioning shaft 32 can be provided between the main body 20 and the support frame 30. The support frame 30 is horizontally arranged, and the positioning shaft 32 is arranged at one axial end of the connection between the main body 20 and the support frame 30; in this embodiment, two positioning shafts 32 can also be provided between the main body 20 and the support frame 30. The support frame 30 is horizontally arranged, and the positioning shafts 32 are arranged at the two-side connections of the main body 20 and the support frame 30, so that the support frame 30 rotates more stably relative to the main body 20.

[0073] The positioning part can limit the positions of the positioning shaft 32 and the support frame 30, ensuring the stable connection between the support frame 30 connected thereto and the main body 20 and enabling smooth rotation; the positioning part can be a positioning hole 21, or structures such as a positioning groove or a positioning rib. In this embodiment, the positioning part is the positioning hole 21, and the positioning shaft 32 passes through the positioning hole 21; the positioning shaft 32 is fixedly connected to the positioning hole 21, and the support frame 30 rotates circumferentially around the positioning shaft 32; specifically, one end of the positioning shaft 32 is located in the accommodation space of the main body 20, and the other end passes through the positioning hole 21 and is located outside the main body 20. Of course, in other embodiments, the positioning shaft 32 can also be rotatably connected to the positioning hole 21, and the support frame 30 is fixedly connected to the positioning shaft 32. With the above structure, on the one hand, whether the support frame 30 rotates around the positioning shaft 32 or the support frame 30 and the positioning shaft 32 rotate together in the positioning hole 21, the positioning hole 21 can play a role in limiting the installation of the positioning shaft 32 and the support frame 30.

[0074] As shown in the Figure 3 accompanying drawings, in this embodiment, the positioning shaft 32 has a first inner cavity 321 that penetrates through both ends along its axis; the positioning shaft 32 has a first communication part 322 for connecting with the coil pipe 10, so that the coil pipe 10 communicates with the first inner cavity 321 of the positioning shaft 32. Based on this, the positioning shaft 32 can also be used as a connection joint of the coil pipe 10 to realize the communication between the coil pipe 10 and the first mixing device 3 or the second mixing device 4. Since the positioning shaft 32 is the rotation center 37 of the support frame 30, when the support frame 30 rotates, the distance between the positioning shaft 32 and the bearing surface 31 on the support frame 30 basically does not change. Therefore, if the positioning shaft 32 is connected and fixed to the fixed end 11 of the coil pipe 10, when the support frame 30 rotates, the fixed end 11 of the coil pipe 10 is relatively stationary with respect to the support frame 30, and excessive pulling on the positioning shaft 32 and the fixed end 11 of the coil pipe 10 will not occur, thus preventing damage to the coil pipe 10.

[0075] It should be noted that the positioning shaft 32 can communicate with the coil pipe 10 through the first communication part 322. In actual use, the positioning shaft 32 does not necessarily need to communicate with the coil pipe 10 and can only serve as the rotation center 37 of the support frame 30.

[0076] In some specific embodiments, when the coil pipe 10 is in use, it is in a communicating state with the positioning shaft 32. The positioning shaft 32 can be rotatably connected to the positioning hole 21, and the positioning shaft 32 is fixedly connected to the support frame 30. Thus, during the process of the coil pipe 10 being wound around the support frame 30, the connection part between the coil pipe 10 and the positioning shaft 32 rotates together with the support frame 30, without circumferentially pulling the coil pipe 10 or the positioning shaft 32, and the rotation is smoother, avoiding twisting of the fixed end 11 of the coil pipe 10.

[0077] In some other embodiments, the positioning shaft 32 and the positioning hole 21 are fixedly connected, the positioning shaft 32 and the support frame 30 are rotatably connected, and the coil pipe 10 is in a communicating state with the positioning shaft 32 during use. Then, during the winding process of the coil pipe 10, the fixed end of the coil pipe 10 and the positioning shaft 32 are not completely fixed, only axially pre-positioned. At this time, the distance between the fixed end 11 of the coil pipe 10 and the bearing surface 31 on the support frame remains unchanged. The support frame 30 rotates relative to the positioning shaft, and the free end of the coil pipe 10 will be evenly wound on the support frame 30. The fixed end 11 of the coil pipe 10 is sleeved on the first communicating portion 322 of the positioning shaft 32 and rotates relative to the positioning shaft following the support frame 30. If the fixed end 11 of the coil pipe 10 is distorted during the rotation process, after the coil pipe 10 is wound, the positioning shaft 32 or the support frame 30 with the wound coil pipe 10 can be removed, the fixed end 11 of the coil pipe 10 can be restored, and then it is completely fixed to the first communicating portion 322 of the positioning shaft 32 again to achieve the communication between the positioning shaft 32 and the coil pipe 10; or, when the coil pipe 10 is wound, the fixed end 11 of the coil pipe 10 is not connected to the positioning shaft 32, but after the coil pipe 10 is completely wound, the fixed end 11 is connected to the positioning shaft 32 to achieve the communication between the positioning shaft 32 and the coil pipe 10.

[0078] In this embodiment, one end of the positioning shaft 32 is in communication with the coil pipe 10, and the other end is in communication with the external first mixing device 3 or second mixing device 4 after the coil pipe 10 is wound. Generally, the other end of the positioning shaft 32 is exposed outside the main body 20 to facilitate the communication with the first mixing device 3 or second mixing device 4; the positioning shaft 32 and the positioning hole 21 can be fixedly connected to ensure that during the use of the coil pipe rack and the coil pipe 10, the connection between the positioning shaft 32 and the coil pipe 10, the first mixing device 3 or the second mixing device 4 does not rotate, ensuring the stable connection between the positioning shaft 32 and the coil pipe 10, and between the positioning shaft 32 and the first mixing device 3 or the second mixing device 4.

[0079] Preferably, when the positioning shaft 32 is rotatably connected to the support frame 30, a wear-resistant member 323 is provided between the positioning shaft 32 and the support frame 30. Thus, the wear-resistant member 323 can reduce the rotational friction between the positioning shaft 32 and the support frame 30, extend the service life of the positioning shaft 32 and the support frame 30, and at the same time protect the first inner cavity 321 in the positioning shaft 32 to avoid leakage at the connection between the coil pipe 10 and the first mixing device 3 or the second mixing device 4 at the positioning shaft 32.

[0080] After the bearing surface 31 is covered by the coil pipe 10, a winding center 22 is formed, and a covering area 23 is formed after the bearing surface 31 is covered by the coil pipe 10. The covering area 23 is the axial projection area after the coil pipe 10 is wound on the support frame 30, and the winding center 22 is actually the center of the projection area.

[0081] In some embodiments, as shown in the attached Figure 4As shown, the winding center 22 is concentrically arranged with the positioning shaft 32, the bearing surface 31 is circumferentially distributed around the positioning shaft 32. After the coil pipe 10 is wound on the support frame 30, it is evenly distributed around the positioning shaft 32, and the weight is relatively balanced.

[0082] In some other embodiments, as shown in the appended Figure 5 and the appended Figure 6 figures, the winding center 22 is eccentrically arranged with the positioning shaft 32, the bearing surface 31 is eccentrically arranged relative to the positioning shaft 32. After the coil pipe 10 is wound on the support frame 30, it is also eccentrically arranged relative to the positioning shaft 32, so that the coil pipe 10 is naturally located in the lower half of the accommodation space when placed in the main body 20, which is beneficial to protecting the coil pipe 10.

[0083] Based on the above several embodiments, there are also different effects corresponding to different positions of the first communication part 322. Specifically, in the two embodiments shown in the appended Figure 4 and the appended Figure 5 figures, the first communication part 322 is located within the covering area 23, that is, the positioning shaft 32 is located within the covering area 23, and the connection part between the coil pipe 10 and the positioning shaft 32 is also located within the covering area 23. After the coil pipe 10 is wound on the support frame 30, the first communication part 322 can be covered, thus avoiding accidental damage to the connection part between the coil pipe 10 and the positioning shaft 32 and ensuring the connection stability between the coil pipe 10, the positioning shaft 32 and other devices. In the embodiment shown in the appended Figure 6 figure, the first communication part 322 is located outside the covering area 23. For the free end of the coil pipe 10, after the coil pipe 10 is wound, it is not easy to be placed into the covering area 23, and it is more convenient to communicate with the first communication part 322 located outside the covering area 23.

[0084] In the above embodiments, the positioning shaft 32 is used as the communication component connecting the coil pipe 10. In other embodiments, referring back to Figure 2 and Figure 3 , the communication component is an independent component. The main body 20 is also provided with at least one communication pipe 24. The communication pipe 24 passes through the main body 20 along its axis direction. The communication pipe 24 has a second inner cavity 241 penetrating through its two ends along its axis. The communication pipe 24 also has a second communication part 242 placed in the accommodation space for connecting with the coil pipe 10, so that the coil pipe 10 is communicated with the second inner cavity 241. The positioning shaft 32 and the communication pipe 24 are not the same pipe. The coil pipe 10 may not be communicated with the positioning shaft 32, but is communicated with the first mixing device 3 or the second mixing device 4 through the communication pipe 24. The installation position of the communication pipe 24 is more flexible. The user can select the position of the communication pipe 24 according to his own needs, and the installation is more convenient and applicable to different first mixing devices 3 and second mixing devices 4.

[0085] Of course, after the coil pipe 10 is wound around the support frame 30, the connecting pipe 24 is located inside the covering area 23, or the connecting pipe 24 is located outside the covering area 23. Specifically, several installation situations are generated therefrom: the connecting pipe 24 and the positioning shaft 32 are integrated and both are located inside the covering area 23 of the coil pipe 10; the connecting pipe 24 and the positioning shaft 32 are integrated and both are no longer inside the covering area 23 of the coil pipe 10; the connecting pipe 24 and the positioning shaft 32 are two components, the connecting pipe 24 is inside the covering area 23 of the coil pipe 10, and the positioning shaft 32 is not inside the covering area 23; the connecting pipe 24 and the positioning shaft 32 are two components, the connecting pipe 24 is not inside the covering area 23 of the coil pipe 10, and the positioning shaft 32 is inside the covering area 23. The user can select whether to set the connecting pipe 24 and the connection mode of the coil pipe 10 according to the requirements of their own equipment.

[0086] As shown in the Figure 3 embodiment shown, the winding center 22 and the positioning shaft 32 are concentrically arranged. The positioning shaft 32 located at one axial end of the support frame 30 has a first connecting part 322 for connecting with one end of the coil pipe 10, and the first connecting part 322 is located inside the covering area 23. This end of the coil pipe 10 is the fixed end 11; a connecting pipe 24 is provided on the main body 20 located at the other axial end of the support frame 30. The connecting pipe 24 passes through the main body 20 along its axial direction. The connecting pipe 24 has a second inner cavity 241 penetrating through both ends along its axis and a second connecting part 242 for connecting with the other end of the coil pipe 10. The connecting pipe 24 and the second connecting part 242 are located outside the covering area 23. This end of the coil pipe 10 is the free end 12. Thus, the effects of protecting the fixed end 11 of the coil pipe 10 and facilitating the installation of the free end 12 can be taken into account.

[0087] Preferably, the positioning shaft 32, the winding center 22, and the rotation center 37 are all horizontally arranged. On the one hand, during the process of winding the coil pipe 10 around the support frame 30, the support frame 30 is horizontally arranged, which is convenient for the coil pipe 10 to be evenly wound, and it is more labor-saving for the operator to rotate the support frame 30; on the other hand, the wound coil pipe 10 is also in a horizontal state during use, and the coil pipe 10 is evenly stressed everywhere, avoiding the coil pipe 10 from being stacked and deformed due to uneven stress everywhere.

[0088] Preferably, among all the positioning shafts 32, the size of the first inner cavity 321 is at least two kinds. The positioning shafts 32 with different sizes of the first inner cavity 321 can be correspondingly connected to coil pipes 10 with different inner diameters, so that the coil pipe rack is applicable to coil pipes 10 with different inner diameters. A variety of positioning shafts 32 with different first inner cavities 321 can be prepared. These positioning shafts 32 can be simultaneously arranged on the main body 20, or a positioning shaft 32 with a suitable size of the first inner cavity 321 can be selected by replacement and installed on the main body 20 and connected to the coil pipe 10.

[0089] As shown in the Figure 2In the illustrated embodiment, the support frame 30 includes at least one support plate 34 and at least two support rods 35 extending axially along the positioning shaft 32. A plurality of support rods 35 are connected to the support plate 34, and the bearing surface 31 is located on the surface of the support rods 35 and the inner side surface where the support plate 34 is connected to the support rods 35. The support frame 30 is in an assembled form, which is conducive to a simple and feasible processing process; the coiled pipe 10 can be evenly wound around the support rods 35, and the axial two ends of the wound coiled pipe 10 can abut against the inner side surface of the support plate 34 to axially limit the coiled pipe 10 and further stabilize the coiled pipe 10. In this embodiment, for the fixed end 11 and the free end 12 of the coiled pipe 10, with the winding direction of the coiled pipe 10 as the reference, the first-fixed end is the fixed end 11, and the free end during the winding process is the free end 12, that is, the last-fixed end is the free end 12.

[0090] Specifically, the number of support plates 34 is two, which are respectively located at the axial two ends of the support rods 35. The number of support rods 35 is greater than three and is evenly distributed circumferentially. As shown in the appendix Figure 4 As shown, the number of support rods 35 is 5, which are evenly distributed circumferentially with the positioning shaft 32 as the center. Based on the winding method of the coiled pipe 10, whether it is the bearing surface 31 on the support rods 35 or the bearing surface 31 on the inner side surface of the support plate 34 in contact with the coiled pipe 10, they are all evenly distributed circumferentially with the positioning shaft 32 as the center. The coiled pipe 10 is wound with the positioning shaft 32 as the center, and it is more stable and labor-saving when rotating the support frame 30.

[0091] As shown in the appendix Figure 5 In the specific example shown, the number of support rods 35 is 5, which are eccentrically arranged on the support plate 34 relative to the axis of the positioning shaft 32, that is, the bearing surface 31 is eccentrically arranged relative to the positioning shaft 32. After the coiled pipe 10 is wound on the support frame 30, it is also eccentrically arranged relative to the positioning shaft 32, so that when the coiled pipe 10 is placed in the main body 20, it naturally lies in the lower half of the accommodation space under the action of gravity, which is conducive to protecting the coiled pipe 10.

[0092] As shown in the appendix Figure 6 In the specific example shown, the number of support rods 35 is 4, which are eccentrically symmetrically arranged with respect to the positioning shaft 32, that is, the bearing surface 31 is eccentrically arranged relative to the positioning shaft 32. After the coiled pipe 10 is wound on the support frame 30, it is also eccentrically arranged relative to the positioning shaft 32, so that when the coiled pipe 10 is placed in the main body 20, it naturally lies in the lower half of the accommodation space under the action of gravity, which is conducive to protecting the coiled pipe 10.

[0093] Regardless of whether the support rods 35 are evenly distributed circumferentially or eccentrically arranged relative to the positioning shaft 32, the support rods 35 are axially symmetrically distributed, ensuring the uniformity of the position of the coiled pipe 10 on the support frame 30, avoiding partial indentation and causing the pipe body to fold or twist, etc. And a plurality of support rods 35 provide a suitable winding space for the coiled pipe 10, avoiding damage to the turning position due to too small a winding radius of the coiled pipe 10 and affecting the internal flow channel of the coiled pipe 10.

[0094] As shown in the appended Figure 3 figure, there is a circumferential gap 351 between multiple support rods 35. On the one hand, it can reduce the weight of the support frame 30. On the other hand, in the embodiment where the positioning shaft 32 communicates with the coil pipe 10, the circumferential gap 351 also allows the fixed end 11 of the coil pipe 10 to pass through between the support rods 35 and communicate with the positioning shaft 32.

[0095] In some embodiments, the support rod 35 and the support plate 34 are detachably fixed and can be fixed after moving to the set position, and the size of the enclosure formed by the support rods 35 can be determined as needed; for the coil pipe 10 with a longer length, the support rods 35 can be relatively far away from each other, so that the winding radius of the coil pipe 10 is larger and it can be wound only in one layer, avoiding the coil pipe 10 being wound in multiple layers and causing stacking and extrusion with each other, which affects the size of the internal flow channel of the coil pipe 10; for the coil pipe 10 with a shorter length, the support rods 35 can be relatively close to each other, so that the coil pipe 10 can be distributed as fully as possible on the surface surrounded by the multiple support rods 35, and both axial sides of the coil pipe 10 are abutted against the two support plates 34 respectively, avoiding leaving extra space on the support frame 30 and causing the coil pipe 10 to axially move on the support rods 35, pulling the connection parts at both ends of the coil pipe 10 and affecting the stable connection between the coil pipe 10 and the positioning shaft 32 or the connecting pipe 24.

[0096] Specifically, mounting holes at different positions or radially extending chutes can be provided on the support plate 34. The support rod 35 is fixedly connected to different mounting holes, or is fixed after sliding to the set position in the chute, both of which can realize the adjustment of the size of the enclosure formed by the support rods 35. In practical applications, other conventional structures can also be used to realize the adjustment of the size of the enclosure formed by the support rods 35.

[0097] As shown in the appended Figure 4 figure, the bearing surface 31 formed on the surface of the support rod 35 is a continuous closed surface, avoiding sharp protrusions on the surface of the support rod 35 that may damage the coil pipe 10, and the coil pipe 10 can be wound more smoothly on the support rod 35.

[0098] As shown in the appended Figure 2 figure, the main body 20 includes two vertical plates 25 and an enclosing member 26. The two vertical plates 25 are arranged opposite to each other along the axial direction of the support frame 30. The vertical plates 25 and the enclosing member 26 form an accommodating space with one side open. The support frame 30 is rotatably arranged inside the main body 20, and at least part of the coil pipe 10 is allowed to be filled into the accommodating space as the support frame 30 rotates; the vertical plates 25 and the enclosing member 26 enclose the support frame 30, thereby forming an accommodating space to enclose the coil pipe 10 and protecting the coil pipe 10 from external damage. The vertical plate 25 is also used for installing the positioning shaft 32 and the support frame 30.

[0099] The support frame 30 and the main body 20 are provided with a number of openings 36, making the whole coil rack lightweight and facilitating transportation. At the same time, the relatively light weight of the support frame 30 also makes it more labor-saving and convenient to operate when winding the coil 10 around the support frame 30. Of course, in some embodiments, the openings 36 may be provided only on the support frame 30 or the main body 20.

[0100] As shown in the Figure 2 accompanying drawings, the cell lysis system further includes a control cabinet 40 carrying a software control system, and the coil rack is fitted with the control cabinet 40, which is beneficial to the structural intensification of the whole cell lysis system. The control cabinet 40 is a hollow cabinet structure, and internally has a controller for controlling the whole cell lysis system, facilitating the electrical connection of devices such as the bacterial liquid supply device 1, the lysis liquid supply device 2, and the neutralization liquid supply device 5 to the controller in the vicinity.

[0101] The above embodiments are only the preferred embodiments of the present invention, and the scope of protection of the present invention cannot be limited thereby. Any non-substantive changes and substitutions made by those skilled in the art based on the present invention fall within the scope of protection required by the present invention.

Claims

1. A cell lysis system, comprising a bacterial liquid supply device, a lysis liquid supply device, a first mixing device, a second mixing device, and a neutralizing liquid supply device, wherein the bacterial liquid supply device and the lysis liquid supply device are both connected to the first mixing device, the first mixing device also has a liquid outlet for communicating with a liquid inlet of a coil, the neutralizing liquid supply device is connected to the second mixing device, and the second mixing device also has a liquid inlet for communicating with a liquid outlet of the coil; It is characterized in that Also included is a coil rack, the coil rack comprising: main body; A support frame, rotatably connected to the main body, and forming a receiving area between the support frame and the main body; The support frame has a bearing surface for bearing the coil, so that when the support frame rotates relative to the main body, the coil surrounds and covers the bearing surface and at least partially fills the accommodating area.

2. The cell lysis system according to claim 1, characterized in that At least one positioning shaft is provided between the main body and the support frame, the main body is provided with a positioning portion to limit the position of the positioning shaft, and the support frame rotates about the positioning shaft to form a rotation center; The positioning shaft has a first inner cavity running through both ends of the positioning shaft along its axis; the positioning shaft has a first connecting portion for connecting with the coil, so that the coil is connected with the first inner cavity of the positioning shaft.

3. The cell lysis system according to claim 2, characterized in that The bearing surface is covered by the coil to form a winding center, and the bearing surface is covered by the coil to form a covering area; The winding center is concentrically arranged with the positioning axis; or, the winding center is eccentrically arranged with the positioning axis.

4. The cell lysis system according to claim 3, characterized in that The first connecting portion is located within the coverage area.

5. The cell lysis system according to claim 3, characterized in that The main body is also provided with at least one connecting pipe, which passes through the main body along its axial direction. The connecting pipe has a second inner cavity that runs through both ends along its axis. The connecting pipe also has a second connecting portion placed in the accommodating space for connecting with the coil, so that the coil is connected with the second inner cavity.

6. The cell lysis system according to claim 5, characterized in that After the coil is wound on the support frame, the connecting pipe is located in the covering area, or the connecting pipe is located outside the covering area.

7. The cell lysis system according to claim 3, characterized in that When the winding center is arranged concentrically with the positioning shaft, the positioning shaft located at one axial end of the support frame has the first connecting portion for connecting with one end of the coil, and the first connecting portion is located in the covering area; A connecting pipe is provided on the main body located at the other axial end of the support frame, and the connecting pipe passes through the main body along its axial direction. The connecting pipe has a second inner cavity running through both ends along its axis and a second connecting part for connecting with the other end of the coil, and the connecting pipe and the second connecting part are located outside the covering area.

8. The cell lysis system according to claim 2, characterized in that The positioning portion is a positioning hole, and the positioning shaft passes through the positioning hole; The positioning shaft is fixedly connected to the positioning hole, and the support frame rotates circumferentially around the positioning shaft; Alternatively, the positioning shaft is rotatably connected to the positioning hole, and the support frame is fixedly connected to the positioning shaft.

9. The cell lysis system according to claim 8, characterized in that When the positioning shaft is rotatably connected to the support frame, a wear-resistant part is provided between the positioning shaft and the support frame.

10. The cell lysis system according to claim 3, characterized in that The positioning axis, the winding center and the rotation center are all arranged horizontally.

11. The cell lysis system according to claim 2, characterized in that: In all the positioning shafts, the first inner cavity has at least two sizes.

12. The cell lysis system according to claim 2, characterized in that The support frame includes at least one support plate and at least two support rods extending axially along the positioning axis, multiple support rods are connected to the support plate, and the bearing surface is located on the surface of the support rod and the inner side surface where the support plate is connected to the support rod.

13. The cell lysis system according to claim 12, characterized in that The plurality of support rods are evenly distributed circumferentially with the axis of the positioning shaft as the center; or, the plurality of support rods are eccentrically arranged on the support plate relative to the axis of the positioning shaft.

14. The cell lysis system according to claim 12, characterized in that A circumferential gap is provided between the plurality of support rods, and / or the support rods and the support plate are detachably fixed and can be fixed after being moved to a set position.

15. The cell lysis system according to claim 12, characterized in that The bearing surface formed on the surface of the support rod is a continuous closed curved surface.

16. The cell lysis system according to claim 1, characterized in that The main body includes two vertical plates and a surrounding member, the two vertical plates are arranged opposite to each other along the axial direction of the support frame, the vertical plates and the surrounding member form a receiving space with one side open, and as the support frame rotates, the coil is allowed to at least partially fill into the receiving space; and / or; The support frame and / or the main body are provided with a plurality of openings.

17. The cell lysis system according to claim 1, characterized in that It also includes a control cabinet that carries a software control system, and the coil rack is embedded in the control cabinet.

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