Rapid separation device for peripheral blood mononuclear cells

Abstract

The utility model discloses a peripheral blood mononuclear cell rapid separation device which comprises a machine body, a centrifugal cylinder is rotatably connected to the inner wall of the machine body, an electric control valve is installed on the inner bottom wall of the centrifugal cylinder, an outer gear ring is fixedly installed on the outer side of the centrifugal cylinder, a driving motor is fixedly installed on the top of the machine body, and a driving motor is fixedly installed on the outer side of the driving motor. An output shaft of the driving motor penetrates through the machine body and is meshed with the outer gear ring through a gear, and an inner gear ring is fixedly installed on the inner wall of the centrifugal cylinder. According to the utility model, the gear rings are arranged on the inner side and the outer side of the centrifugal cylinder, the synchronous rotation of the plurality of groups of stirring shafts and the centrifugal cylinder is realized by the single group of driving motors, and the rotating directions of the plurality of groups of stirring shafts are inconsistent, so that sufficient stirring can be realized, and the separation efficiency of mononuclear cell discharge can be improved and cell separation can be realized by matching with the rotating centrifugal cylinder; meanwhile, by arranging the temperature control assembly, the internal temperature of the machine body can be kept within a set interval according to specific situations, and cell separation is better facilitated.

Description

Technical Field

[0001] The utility model relates to the technical field of biological cell separation, and particularly relates to a rapid separation device for peripheral blood mononuclear cells. Background Technique

[0002] Peripheral blood mononuclear cells are cells with a single nucleus in peripheral blood, including lymphocytes and monocytes; the main separation method for peripheral blood mononuclear cells is density gradient centrifugation; peripheral blood mononuclear cells include lymphocytes and monocytes; the volume, morphology, and specific gravity of mononuclear cells are different from other cells in peripheral blood. The specific gravity of red blood cells and polymorphonuclear leukocytes is about 1.092, the specific gravity of mononuclear cells is 1.075 - 1.090, and that of platelets is 1.030 - 1.035; therefore, by using a solution with an osmotic pressure close to isotonicity between 1.075 and 1.092 for density gradient centrifugation, cells with a certain density can be distributed according to the corresponding density gradient, and various blood cells can be separated from mononuclear cells.

[0003] Peripheral blood mononuclear cells include lymphocytes and monocytes, with a specific gravity of about 1.070, while the specific gravity of red blood cells and polymorphonuclear leukocytes exceeds 1.080; thus, by using a solution with a specific gravity between the two for density gradient centrifugation, mononuclear cells can be obtained; the most commonly used layering solution is a solution of sucrose and sodium diatrizoate with a specific gravity of 1.070 ± 0.001, and meglumine diatrizoate is commonly used in China to replace sodium diatrizoate.

[0004] After retrieval, a patent with the application number CN201922205534.9 discloses a rapid separation device for peripheral blood mononuclear cells, including a machine body. The top of the machine body is helically installed with a machine cover. The surface of the machine cover is installed with a rotating rod, the top of the machine cover is installed with a motor, one side of the motor is installed with a liquid medicine tank, the bottom of the liquid medicine tank is installed with a liquid outlet pipe, the surface of the machine body is installed with a power supply, the bottom of the machine body is installed with support legs, and one side of the rotating column is installed with a first filter screen. The liquid medicine can directly enter the interior of the machine body through the liquid medicine tank and the liquid outlet pipe. The stirring blades on the surface of the rotating column can fully mix the peripheral blood mononuclear cell solution and the liquid medicine. The peripheral blood mononuclear cell solution can be fully separated through the first filter screen and the second filter screen. The interior of the machine body can be fully heated through the electric heating net and the heat conduction plate to make the separation effect better. When the separation is completed, the machine cover can be opened through the helically installed machine cover to facilitate cleaning the interior of the machine body.

[0005] This structure realizes cell separation by adding peripheral blood solution into the interior of the first filter net and then adding the medicinal solution for stirring to obtain mononuclear cells. However, the stirring effect in this structure is not good, and there is a lack of power to discharge the mononuclear cells from the first filter net after separation, resulting in a poor separation effect. Moreover, after the electric heating net in this structure is turned on, it is not convenient to control the temperature, so that the internal temperature of the body cannot be controlled within the set range, affecting the separation efficiency. Summary of the Utility Model

[0006] Aiming at the problems existing in the prior art, the purpose of the present utility model is to provide a rapid separation device for peripheral blood mononuclear cells to solve the problems in the background technology.

[0007] To achieve the above purpose, the present utility model adopts the following technical solutions;

[0008] A rapid separation device for peripheral blood mononuclear cells, including a body. An inner wall of the body is rotatably connected with a centrifuge cylinder. An electric control valve is installed on an inner bottom wall of the centrifuge cylinder. An outer gear ring is fixedly installed on an outer side of the centrifuge cylinder. A driving motor is fixedly installed on a top of the body. An output shaft of the driving motor penetrates through the body and meshes with the outer gear ring through a gear. An inner gear ring is fixedly installed on an inner wall of the centrifuge cylinder. Synchronous gears are rotatably connected to an inner top wall of the body at even intervals. The synchronous gears mesh with the inner gear ring. A stirring shaft is fixedly connected to a bottom of the synchronous gears. A feeding pipe and a medicinal solution pipe are fixedly connected to a top of the body. A guiding groove is formed on an inner bottom wall of the body. A discharge pipe is connected to an inner bottom wall of the guiding groove. A temperature control component is arranged inside the body;

[0009] The temperature control component includes an electric heating net installed on an inner wall of the body. A temperature sensor and a processor are installed on the inner wall of the body. The temperature sensor and the electric heating net are both in signal connection with the processor.

[0010] As a further description of the above technical solution: A push plate is fixedly connected to an outer side of the centrifuge cylinder. The push plate is located inside the guiding groove.

[0011] As a further description of the above technical solution: A status indicating lamp is fixedly installed on a top of the body. The status indicating lamp is in signal connection with the processor.

[0012] As a further description of the above technical solution: A control panel is fixedly installed on an outer side of the body. The electric heating net, the driving motor and the electric control valve are all electrically connected to the control panel.

[0013] As a further description of the above technical solution: Hinged covers are installed at tops of the feeding pipe and the medicinal solution pipe. Handles are installed on the covers.

[0014] As a further description of the above technical solution: The connection position between the centrifugal cylinder and the machine body is rotationally sealed.

[0015] Compared with the prior art, the advantages of the present utility model are as follows:

[0016] In the present utility model, by arranging tooth rings on both the inner and outer sides of the centrifugal cylinder, a single set of drive motors is used to achieve the synchronous rotation of multiple stirring shafts and the centrifugal cylinder, and the rotation directions of the multiple stirring shafts are inconsistent, which can achieve sufficient stirring. Cooperating with the rotating centrifugal cylinder can improve the separation efficiency of monocyte discharge and achieve cell separation. At the same time, by setting the temperature control component, the temperature inside the machine body can be maintained within a set range according to specific situations, which is more conducive to cell separation. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 is a three-dimensional structural schematic diagram of the present utility model;

[0018] Figure 2 is a front-view sectional structural schematic diagram of the present utility model;

[0019] Figure 3 is for the Figure 2 enlarged structural schematic diagram at position A in the present utility model;

[0020] Figure 4 is a schematic diagram of the principle of the present utility model.

[0021] Description of the reference numerals in the drawings:

[0022] 1. Machine body; 2. Centrifugal cylinder; 3. Electric control valve; 4. Outer tooth ring; 5. Drive motor; 6. Inner tooth ring; 7. Synchronous gear; 8. Stirring shaft; 9. Feeding pipe; 10. Liquid medicine pipe; 11. Guide groove; 12. Discharge pipe; 13. Temperature control component; 1301. Electric heating mesh; 1302. Temperature sensor; 1303. Processor; 14. Push plate; 15. Status indicator light; 16. Control panel; 17. Cover plate. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0023] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the drawings in the embodiments of the present utility model.

[0024] Please refer to Figures 1 to 4, in the present utility model, a rapid separation device for peripheral blood mononuclear cells includes a body 1. A centrifuge cylinder 2 is rotatably connected to the inner wall of the body 1. An electric control valve 3 is installed on the inner bottom wall of the centrifuge cylinder 2. An external gear ring 4 is fixedly installed on the outside of the centrifuge cylinder 2. A driving motor 5 is fixedly installed on the top of the body 1. The output shaft of the driving motor 5 penetrates through the body 1 and meshes with the external gear ring 4 through a gear. An internal gear ring 6 is fixedly installed on the inner wall of the centrifuge cylinder 2. Uniformly distributed synchronous gears 7 are rotatably connected to the inner top wall of the body 1. The synchronous gears 7 mesh with the internal gear ring 6. A stirring shaft 8 is fixedly connected to the bottom of the synchronous gears 7. A feeding pipe 9 and a liquid medicine pipe 10 are fixedly connected to the top of the body 1. A guiding groove 11 is opened on the inner bottom wall of the body 1. A discharge pipe 12 is connected to the inner bottom wall of the guiding groove 11. A temperature control component 13 is arranged inside the body 1.

[0025] The temperature control component 13 includes an electric heating net 1301, which is installed on the inner wall of the body 1. A temperature sensor 1302 and a processor 1303 are installed on the inner wall of the body 1. The temperature sensor 1302 and the electric heating net 1301 are both in signal connection with the processor 1303.

[0026] Hinged covers 17 are installed at the tops of the feeding pipe 9 and the liquid medicine pipe 10, and handles are installed on the covers 17;

[0027] The connection position between the centrifuge cylinder 2 and the body 1 is rotationally sealed.

[0028] A valve is installed on the discharge pipe 12 to achieve sealing. When it is necessary to complete the separation of peripheral blood mononuclear cells, the user introduces the peripheral blood cell solution into the interior of the body 1 through the addition pipe 9, and then closes the cover plate 17 to achieve sealing. At this time, the cell solution is inside the centrifuge tube 2. Subsequently, a medicinal solution is added into the interior of the body 1 through the top medicinal solution pipe 10. Then, the temperature control component 13 and the drive motor 5 are controlled to operate. The temperature sensor 1302 monitors the temperature of the solution inside the body 1 in real time and transmits the value to the processor 1303. The processor 1303 controls the electrothermal grid 1301 to be energized, causing the temperature of the electrothermal grid 1301 to rise, thereby heating the solution. Due to the setting of the temperature sensor 1302, temperature monitoring can be achieved. The processor 1303 can change the energy consumption of the electrothermal grid 1301 according to the set temperature to keep the internal solution within the set range. When the drive motor 5 is energized to work, it drives the gear fixedly connected thereto to rotate, pushing the external gear ring 4 to rotate synchronously, so that the centrifuge tube 2 and the inner internal gear ring 6 rotate synchronously. Driven by the internal gear ring 6, multiple synchronous gears 7 rotate simultaneously, and then multiple stirring shafts 8 rotate synchronously. Moreover, the multiple stirring shafts 8 rotate in different directions, which can achieve efficient stirring of the solution. Subsequently, the mononuclear cell solution overflows to the top of the guiding groove 11 through the holes on the centrifuge tube 2, while lymphocytes and the like remain inside the centrifuge tube 2. After the separation is completed, the separated peripheral blood mononuclear cells can be obtained by opening the valve on the discharge pipe 12, and the part inside the centrifuge tube 2 is discharged through the electric control valve 3.

[0029] In the present utility model, by arranging gear rings on both the inner and outer sides of the centrifuge tube 2, a single drive motor 5 realizes the synchronous rotation of multiple stirring shafts 8 and the centrifuge tube 2, and the multiple stirring shafts 8 rotate in different directions, which can achieve sufficient stirring. Cooperating with the rotating centrifuge tube 2 can improve the separation efficiency of the discharge of mononuclear cells, realize cell separation. At the same time, by setting the temperature control component 13, the temperature inside the body 1 can be maintained within the set range according to specific situations, which is more conducive to cell separation.

[0030] Please refer to Figure 2 , wherein: a push plate 14 is fixedly connected to the outer side of the centrifuge tube 2, and the push plate 14 is located inside the guiding groove 11.

[0031] In the present utility model, when the centrifuge tube 2 rotates, the push plate 14 will also rotate accordingly. The push plate 14 is inside the guiding groove 11 to push the peripheral blood cells to move to the position of the discharge pipe 12 for discharge, preventing slow discharge or accumulation.

[0032] Please refer to Figure 1 and 4 , wherein: a status indicating lamp 15 is fixedly installed on the top of the body 1, and the status indicating lamp 15 is signal-connected to the processor 1303.

[0033] In the present utility model, during the operation of the temperature control component 13, the processor 1303 will control the operation of the status indicator light 15 in real time to generate corresponding colors, so that the user can timely know whether the temperature control component 13 is operating normally.

[0034] Please refer to Figure 1 , wherein: a control panel 16 is fixedly installed on the outer side of the body 1, and the electric heating grid 1301, the drive motor 5 and the electric control valve 3 are all electrically connected to the control panel 16.

[0035] In the present utility model, the setting of the control panel 16 enables the user to operate this device more simply and quickly, thereby reducing the steps that the personnel need to operate, and thus improving the separation efficiency of peripheral blood mononuclear cells.

[0036] The above is only the preferred specific implementation manner of the present utility model; however, the protection scope of the present utility model is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present utility model, according to the technical solution of the present utility model and its improved concept, makes equivalent substitutions or changes, and should be covered by the protection scope of the present utility model.

Claims

1. A peripheral blood mononuclear cell rapid separation device, comprising a body (1), characterized in that: A centrifugal cylinder (2) is rotatably connected to the inner wall of the machine body (1), an electric control valve (3) is installed on the inner bottom wall of the centrifugal cylinder (2), an outer gear ring (4) is fixedly installed on the outer side of the centrifugal cylinder (2), a driving motor (5) is fixedly installed on the top of the machine body (1), an output shaft of the driving motor (5) passes through the machine body (1) and meshes with the outer gear ring (4) through a gear, an inner gear ring (6) is fixedly installed on the inner wall of the centrifugal cylinder (2), and an inner top wall of the machine body (1) is fixedly installed with a gear. The upper part of the machine body (1) is rotatably connected to evenly distributed synchronous gears (7), the synchronous gears (7) mesh with the inner gear ring (6), the bottom of the synchronous gears (7) is fixedly connected to a stirring shaft (8), the top of the machine body (1) is fixedly connected to an addition pipe (9) and a liquid medicine pipe (10), a guide groove (11) is provided on the inner bottom wall of the machine body (1), a discharge pipe (12) is connected to the inner bottom wall of the guide groove (11), and a temperature control component (13) is provided inside the machine body (1); The temperature control component (13) comprises an electric heating network (1301), the electric heating network (1301) is installed on the inner wall of the machine body (1), a temperature sensor (1302) and a processor (1303) are installed on the inner wall of the machine body (1), and the temperature sensor (1302) and the electric heating network (1301) are both connected to the processor (1303) by signal.

2. A peripheral blood mononuclear cell rapid separation device according to claim 1, characterized in that: A push plate (14) is fixedly connected to the outer side of the centrifugal cylinder (2), and the push plate (14) is located inside the guide groove (11).

3. A peripheral blood mononuclear cell rapid separation device according to claim 1, characterized in that: A status indicator light (15) is fixedly mounted on the top of the machine body (1), and the status indicator light (15) is connected to the processor (1303) by signal.

4. A peripheral blood mononuclear cell rapid separation device according to claim 1, characterized in that: A control panel (16) is fixedly mounted on the outer side of the machine body (1), and the electric heating network (1301), the drive motor (5) and the electric control valve (3) are all electrically connected to the control panel (16).

5. The device for rapid separation of peripheral blood mononuclear cells according to claim 1, characterized in that: The top ends of the addition tube (9) and the medicine liquid tube (10) are both hingedly connected with a cover plate (17), and a handle is installed on the cover plate (17).

6. A peripheral blood mononuclear cell rapid separation device according to claim 1, characterized in that: The connection position between the centrifugal cylinder (2) and the machine body (1) is subjected to a rotary sealing treatment.

Images