Culture device for cells

Abstract

The utility model discloses a culture device for cells, which relates to the technical field of cell culture and comprises a culture box, a fixing frame is fixedly connected to the inner wall of the left side of the culture box, a plurality of mounting grooves are formed in the fixing frame, culture test tubes are clamped in the mounting grooves, openings are formed in the tops of the culture test tubes, the openings are sealed by sealing plugs, and the sealing plugs are sleeved on top connecting tubes. The other end of the top connecting pipe is connected with a control valve, the control valve is in threaded connection with the top connecting pipe, the other end of the control valve is in threaded connection with a branch pipe of a multi-way pipe I, and one end of the branch pipe of the multi-way pipe I is fixedly connected with an infusion tube; a control valve is in threaded connection with one end of the bottom connecting pipe away from the culture test tube; according to the utility model, the infusion pump, the infusion pump and the pipelines are matched for use, so that the culture solution does not need to be manually replaced, and bacteria or impurities are prevented from entering the culture solution to influence cell culture in the process of replacing the culture solution.

Description

Technical Field

[0001] The utility model relates to the technical field of cell culture, in particular to a culture device for cells. Background Technique

[0002] Cells (English name: cell) do not have a unified definition. A more common statement is that cells are the basic structural and functional units of organisms. It is known that all organisms except viruses are composed of cells, but the life activities of viruses can only be reflected in cells. Cell culture work has now been widely applied in various fields such as biology, medicine, and new drug research and development, becoming one of the most important basic sciences.

[0003] Cells will produce metabolism during the culture process, and some wastes such as lactic acid and ammonia will be produced during the metabolism process. These metabolic wastes accumulate continuously in the culture medium. When the concentration reaches a certain level, it will have a toxic effect on the cells, inhibiting the growth and function of the cells. At this time, the culture medium needs to be replaced to remove the metabolic wastes.

[0004] Most of the existing replacement of the culture medium relies on manual replacement. Manual replacement is likely to cause bacteria or impurities to enter the cell culture vessels and is also likely to cause damage to the vessels. Content of the Utility Model

[0005] The purpose of the utility model is to provide a culture device for cells to solve the problems put forward in the above background technique.

[0006] To solve the above technical problems, a culture device for cells provided by the utility model includes an incubator. A fixed frame is fixedly connected to the left inner wall of the incubator. The fixed frame is provided with a plurality of installation slots. Culture test tubes are clamped in the installation slots. The tops of the culture test tubes are open and are sealed by a sealing plug. The sealing plug is sleeved on the top connecting pipe. The other end of the top connecting pipe is connected to a control valve. The control valve is threadedly connected to the top connecting pipe. The other end of the control valve is threadedly connected to a branch pipe of a multi-way pipe one. One end of a branch pipe of the multi-way pipe one is fixedly connected to an infusion pipe. A bottom connecting pipe is fixedly connected through the side wall of the bottom of the culture test tube. The end of the bottom connecting pipe away from the culture test tube is threadedly connected to a control valve. The other end of the control valve is threadedly connected to a branch pipe of a multi-way pipe two. One end of a branch pipe of the multi-way pipe two is fixedly connected to a liquid extraction pipe. A support plate is fixedly connected to the inner wall of the incubator on the side away from the fixed frame. A liquid storage tank is fixedly connected to the top of the support plate. An infusion pump is provided at one end of the liquid storage tank. The infusion pump is connected to the liquid storage tank through a pipeline. A pipeline is provided at the top of the infusion pump. The other end of the pipeline is fixedly connected to the infusion pipe. A one-way valve one is provided at the connection. A waste liquid collection tank fixedly connected to the bottom inner wall of the incubator is provided below the support plate. A liquid extraction pump is provided at one end of the waste liquid collection tank. The liquid extraction pump is connected to the waste liquid collection tank through a pipeline. A pipeline is provided at the top of the liquid extraction pump. The other end of the pipeline is fixedly connected to the liquid extraction pipe. A one-way valve two is provided at the connection.

[0007] Further, the number of the mounting grooves is six to eight, and they are horizontally symmetrically arranged on the fixing frame.

[0008] Further, filters are snap-fitted in the plurality of bottom connecting pipes.

[0009] Further, the filter is made of a polycarbonate membrane or a cellulose acetate membrane, and the pore diameter of the filter is between... microns and... microns.

[0010] Further, the check valve one only allows the culture medium to flow from the liquid storage tank along the infusion tube into the culture test tube, and the check valve two only allows the waste liquid to flow from the culture test tube along the extraction tube into the waste liquid collection tank.

[0011] Further, a liquid filling hole is provided on one side wall of the liquid storage tank close to the infusion pump, and a cover plate is provided at the liquid filling hole. The cover plate is threadedly connected to the outer wall of the liquid storage tank.

[0012] Further, sealing rings are provided at the connection between the multi-way pipe one and the infusion tube, the connection between the multi-way pipe two and the extraction tube, the connection between the infusion tube and the top pipe of the infusion pump, and the connection between the extraction tube and the top pipe of the extraction pump.

[0013] Further, the mounting groove is a semi-circular groove vertically symmetrically arranged, and the mounting groove is adapted to the culture test tube.

[0014] Compared with the prior art, the beneficial effects of the present utility model are as follows: By the combined use of the infusion pump, the extraction pump and each pipeline, there is no need for manual replacement of the culture medium, which avoids the entry of bacteria or impurities during the replacement process of the culture medium and affects cell culture; The control valve is used to achieve precise control of the cell culture test tube; By the setting of the check valve, the cells are prevented from being contaminated by the extracted waste liquid. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 is a three-dimensional structural schematic diagram of a cell culture device;

[0016] Figure 2 is a structural schematic diagram of the placement of the culture test tube in a cell culture device;

[0017] Figure 3 is a structural schematic diagram of the liquid storage tank and the waste liquid collection tank in a cell culture device;

[0018] Figure 4 is a cross-sectional view of the bottom connecting pipe in a cell culture device.

[0019] In the figure:

[0020] 10. Incubator;

[0021] 20. Support plate; 21. Liquid storage tank; 22. Waste liquid collection tank; 23. Infusion pump; 24. Liquid extraction pump; 25. Liquid filling hole;

[0022] 30. Fixing frame; 31. Installation groove; 32. Culture test tube; 33. Sealing plug; 34. Top connecting pipe; 35. Bottom connecting pipe; 36. Filter;

[0023] 40. Control valve; 41. Liquid extraction pipe; 42. Multi-way pipe one; 43. Infusion pipe; 44. Multi-way pipe two;

[0024] 45. Check valve one; 46. Check valve two. Detailed implementation manners

[0025] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0026] Please refer to Figures 1-4 , the present invention provides a technical solution:

[0027] Refer to Figures 1-4As shown in the figure, a cell culture device includes an incubator 10. A fixing rack 30 is fixedly connected to the left inner wall of the incubator 10. The fixing rack 30 is provided with a plurality of installation grooves 31. A culture test tube 32 is clamped in the installation groove 31. The top of the culture test tube 32 is open, and the opening is sealed by a sealing plug 33. The sealing plug 33 is sleeved on the top connecting pipe 34. The other end of the top connecting pipe 34 is connected to a control valve 40. The control valve 40 is threadedly connected to the top connecting pipe 34. The other end of the control valve 40 is threadedly connected to a branch pipe of a multi-way pipe 42. One end of a branch pipe of the multi-way pipe 42 is fixedly connected to an infusion pipe 43. A bottom connecting pipe 35 is fixedly connected through the side wall of the bottom of the culture test tube 32. The end of the bottom connecting pipe 35 away from the culture test tube 32 is threadedly connected to a control valve 40. The other end of the control valve 40 is threadedly connected to a branch pipe of a multi-way pipe 44. One end of a branch pipe of the multi-way pipe 44 is fixedly connected to a liquid extraction pipe 41. A support plate 20 is fixedly connected to the inner wall of the incubator 10 on the side away from the fixing rack 30. A liquid storage tank 21 is fixedly connected to the top of the support plate 20. An infusion pump 23 is provided at one end of the liquid storage tank 21. The infusion pump 23 is connected to the liquid storage tank 21 through a pipeline. A pipeline is provided at the top of the infusion pump 23, and the other end of the pipeline is fixedly connected to the infusion pipe 43. A one-way valve 45 is provided at the connection. A waste liquid collection tank 22 fixedly connected to the bottom inner wall of the incubator 10 is provided below the support plate 20. A liquid extraction pump 24 is provided at one end of the waste liquid collection tank 22. The liquid extraction pump 24 is connected to the waste liquid collection tank 22 through a pipeline. A pipeline is provided at the top of the liquid extraction pump 24, and the other end of the pipeline is fixedly connected to the liquid extraction pipe 41. A one-way valve 46 is provided at the connection. The one-way valve 45 only allows the culture medium to flow from the liquid storage tank 21 along the infusion pipe 43 into the culture test tube 32, and the one-way valve 46 only allows the waste liquid to flow from the culture test tube 32 along the liquid extraction pipe 41 into the waste liquid collection tank 22. The flow direction of the liquid is controlled by the one-way valve to create a suitable cell culture environment. The installation groove 31 is a semi-circular groove symmetrically arranged vertically, and the installation groove 31 is adapted to the culture test tube 32. The culture test tube 32 is fixed through the adaptation of the culture test tube 32 and the installation groove 31. The number of the installation grooves 31 is six to eight, and they are symmetrically arranged horizontally on the fixing rack 30.

[0028] Refer to Figures 1-4 As shown in the figure, a filter 36 is clamped in each of the plurality of bottom connecting pipes 35. The filter 36 is made of polycarbonate membrane or cellulose acetate membrane, and the pore diameter of the filter 36 is between 0.2 microns and 0.45 microns. The filter 36 avoids the cells being drawn out together during the liquid extraction process.

[0029] Refer to Figures 1-4As shown in the figure, a cell culture device is provided. A liquid filling hole 25 is provided on a side wall of the liquid storage tank 21 close to the infusion pump 23. A cover plate is provided at the liquid filling hole 25. The cover plate is threadedly connected to the outer wall of the liquid storage tank 21 to cover the liquid filling hole 25 and prevent the culture medium from being contaminated. Sealing rings are provided at the connection between the multi-way pipe 1 42 and the infusion pipe 43, the connection between the multi-way pipe 2 44 and the liquid extraction pipe 41, the connection between the infusion pipe 43 and the top pipe of the infusion pump 23, and the connection between the liquid extraction pipe 41 and the top pipe of the liquid extraction pump 24. The sealing rings are used to seal the connections of each part to achieve a sterile environment.

[0030] Working principle: When culturing cells, first clean and disinfect the culture device, then snap the culture test tube 32 into the installation groove 31 of the fixing frame 30, and then connect the control valve 40 to each pipeline, while paying attention to the sealing condition of the connections; during the cell culture process, when the metabolic waste produced by the cells exceeds the threshold or the application components of the culture medium are too low, it is necessary to replace the culture medium in the culture test tube 32. First, use the liquid extraction pump 24 to extract the waste liquid. The waste liquid enters the waste liquid collection tank 22 through the bottom connecting pipe 35, the multi-way pipe 2 44, and the liquid extraction pipe 41. At this time, the filter 36 in the bottom connecting pipe 35 prevents the cells from being extracted together. Then use the infusion pump 23 to send the culture medium from the liquid storage tank 21 into the culture test tube 32 through the infusion pipe 43.

Claims

1. A cell culture device, comprising an incubator (10), characterized in that: The left inner wall of the incubator (10) is fixedly connected to a fixing frame (30), the fixing frame (30) is provided with a plurality of mounting grooves (31), a culture test tube (32) is clamped in the mounting groove (31), the top of the culture test tube (32) is open, the opening is sealed by a sealing plug (33), the sealing plug (33) is sleeved on the top connecting pipe (34), the other end of the top connecting pipe (34) is connected to a control valve (40), the control valve (40) is threadedly connected to the top connecting pipe (34), the other end of the control valve (40) is threadedly connected to a branch pipe of a multi-way pipe (42), one end of the branch pipe of the multi-way pipe (42) is fixedly connected to the infusion pipe (43), the bottom side wall of the culture test tube (32) is fixedly connected to a bottom connecting pipe (35), one end of the bottom connecting pipe (35) away from the culture test tube (32) is threadedly connected to the control valve (40), the other end of the control valve (40) is threadedly connected to the branch pipe of the multi-way pipe (44), The incubator (10) is connected to the incubator (10) by a plurality of channels, one end of the branch pipe of the multi-channel pipe (44) is fixedly connected to the liquid extraction pipe (41); the inner wall of the incubator (10) on the side away from the fixing frame (30) is fixedly connected to a support plate (20); the top of the support plate (20) is fixedly connected to a liquid storage tank (21); one end of the liquid storage tank (21) is provided with an infusion pump (23); the infusion pump (23) is connected to the liquid storage tank (21) through a pipeline; the top of the infusion pump (23) is provided with a pipeline; the other end of the pipeline is connected to the infusion pipe ( 43) is fixedly connected, and a one-way valve (45) is provided at the connection. A waste liquid collection box (22) fixedly connected to the inner wall of the bottom of the incubator (10) is provided below the support plate (20). A liquid pump (24) is provided at one end of the waste liquid collection box (22). The liquid pump (24) is connected to the waste liquid collection box (22) through a pipeline. A pipeline is provided on the top of the liquid pump (24). The other end of the pipeline is fixedly connected to the liquid extraction pipe (41), and a one-way valve (46) is provided at the connection.

2. A cell culture device according to claim 1, characterized in that: The number of the mounting grooves (31) is six to eight, and they are horizontally symmetrically arranged on the fixing frame (30).

3. A cell culture device as claimed in claim 1, characterized in that: Filters (36) are clamped in each of the multiple bottom connecting pipes (35).

4. A cell culture device as claimed in claim 3, characterized in that: The material of the filter (36) is a polycarbonate membrane or a cellulose acetate membrane, and the pore size of the filter (36) is between 0.2 microns and 0.45 microns.

5. A cell culture device as claimed in claim 1, characterized in that: The one-way valve 1 (45) only allows the culture fluid to flow from the fluid storage tank (21) along the fluid delivery tube (43) into the culture test tube (32), and the one-way valve 2 (46) only allows the waste fluid to flow from the culture test tube (32) along the fluid extraction tube (41) into the waste fluid collection tank (22).

6. A cell culture device as claimed in claim 1, characterized in that: A liquid filling hole (25) is provided on one side wall of the liquid storage box (21) close to the infusion pump (23), and a cover plate is provided at the liquid filling hole (25), and the cover plate is threadedly connected to the outer wall of the liquid storage box (21).

7. A cell culture device as claimed in claim 1, characterized in that: Sealing rings are provided at the connection between the multi-way tube 1 (42) and the infusion tube (43), the connection between the multi-way tube 2 (44) and the extraction tube (41), the connection between the infusion tube (43) and the top pipe of the infusion pump (23), and the connection between the extraction tube (41) and the top pipe of the extraction pump (24).

8. The cell culture device according to claim 1, characterized in that: The mounting groove (31) is a semicircular groove arranged vertically symmetrically, and the mounting groove (31) is adapted to the culture test tube (32).

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