Stem cell culture perfusion device

Abstract

The utility model relates to the technical field of stem cell culture, in particular to a stem cell culture perfusion device which comprises a working table, a nutrition box, an output pipe and a perfusion assembly, the nutrition box is fixedly connected with the working table, the output pipe is fixedly connected with the nutrition box, and the perfusion assembly comprises a containing box, an upper cover, an installation fan, an electromagnetic valve, an opening and closing piece, a driving piece and a plurality of sets of containing pieces. The placing box is fixedly connected with the working table, the upper cover is fixedly connected with the placing box, the mounting fan is detachably connected with the placing box, the electromagnetic valve is fixedly connected with the mounting fan, the opening and closing piece is hinged to the upper cover, the driving piece is fixedly connected with the placing box, and the placing pieces are fixedly connected with the driving piece. The risk of cell infection is effectively reduced, the culture bottle is convenient to place and take out, and the use convenience and practicability of the device are improved.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell culture, in particular to a stem cell culture perfusion device. Background Art

[0002] At present, stem cell tissue fluid needs nutrient solution for cultivation during testing and other medical effects, and a batch of stem cell test tubes needs a certain amount of nutrient solution for cultivation. In the prior art, the perfusion of stem cell nutrient solution is generally performed manually to perfuse multiple culture bottles in sequence. The nutrient solution is prone to leakage during the perfusion process, and the work efficiency is relatively low. In addition, the existing sealing of the culture bottles is generally only performed by sealing plugs, and the sealing effect is relatively insufficient. They need to be opened one by one during perfusion, which complicates the operation and reduces the linkage of the device.

[0003] In order to solve the above problems, the prior art patent (CN217948150U) discloses a stem cell culture perfusion device, comprising a base, a frame body is fixedly connected to the upper end of the base, a fixed plate is fixedly connected between the multiple inner walls of the frame body, a plurality of through holes are provided on the fixed plate, and culture bottles are arranged in the multiple through holes, through grooves are provided on the side walls on both sides of the frame body, a movable plate is slidably connected between the two through grooves, a groove is provided at the lower end of the movable plate, a plurality of perfusion ports arranged at equal intervals are arranged in the groove, and solenoid valves are arranged in the multiple perfusion ports, a feeding mechanism for feeding the multiple perfusion ports is provided at the upper end of the movable plate, and a moving mechanism for moving the movable plate is provided on the right side wall of the frame body; the utility model moves the movable plate so that the multiple perfusion ports at the lower end of the movable plate perfuse the multiple culture bottles in the frame body, thereby improving the perfusion efficiency and avoiding the leakage of nutrient solution during the perfusion process.

[0004] However, in the above-mentioned prior art, when the outermost culture bottle is perfused, the mouth of the innermost culture bottle will be in an open state for a long time, which is likely to increase the risk of cell infection, and the device is not convenient for taking out the culture bottle. Summary of the invention

[0005] The utility model aims to provide a stem cell culture perfusion device, which solves the technical problems in the prior art that when perfusing the outermost culture bottle, the mouth of the innermost culture bottle will be in an open state for a long time, which easily increases the risk of cell infection, and the device is not convenient for taking out the culture bottle.

[0006] To achieve the above object, a stem cell culture perfusion device adopted by the present utility model includes an operating table, a nutrient box, an output pipe, and a perfusion assembly. The nutrient box is fixedly connected to the operating table and is located above the operating table. The output pipe is fixedly connected to the nutrient box and is located outside the nutrient box. The perfusion assembly includes a placement box, an upper cover, an installation fan, a solenoid valve, an opening and closing member, a driving member, and multiple groups of placement members. The placement box is fixedly connected to the operating table and is located outside the nutrient box. The upper cover is fixedly connected to the placement box and is located above the placement box. The installation fan is detachably connected to the placement box and is located outside the upper cover. The solenoid valve is fixedly connected to the installation fan and is located below the output pipe. The opening and closing member is hinged to the upper cover and is located outside the upper cover. The driving member is fixedly connected to the placement box and is located inside the placement box. Multiple groups of the placement members are fixedly connected to the driving member and are located above the driving member.

[0007] Among them, the opening and closing member includes an opening and closing fan, a lifting buckle, and a sealing strip. The opening and closing fan is hinged to the upper cover and is located outside the upper cover. The lifting buckle is fixedly connected to the opening and closing fan and is located above the opening and closing fan. The sealing strip is fixedly connected to the opening and closing fan and is located above the placement box.

[0008] Among them, the driving member includes a driving motor, a driving rod, a positioning seat, and a rotating disk. The output end of the driving motor is fixedly connected to one end of the driving rod and is located inside the placement box. The other end of the driving rod is rotatably connected to the positioning seat and is located above the driving motor. The positioning seat is fixedly connected to the upper cover and is located below the upper cover. The rotating disk is fixedly connected to the driving rod and is located outside the driving rod, and the driving rod penetrates through the rotating disk.

[0009] Among them, each group of the placement members includes a placement plate, a fixing frame, and a wrapping pad. The placement plate is fixedly connected to the rotating disk and is located above the rotating disk. The fixing frame is fixedly connected to the placement plate and is located above the placement plate. The wrapping pad is fixedly connected to the fixing frame and is located inside the fixing frame.

[0010] Among them, the upper cover has a through groove adapted to the installation fan.

[0011] Among them, the stem cell culture perfusion device also includes four groups of auxiliary components, and the four groups of auxiliary components are distributed circumferentially along the axial center line of the rotating disk. Each group of auxiliary components includes a connecting rod, a connecting frame and a roller. The connecting rod is fixedly connected to the rotating disk and is located below the rotating disk. The connecting frame is fixedly connected to the connecting rod and is located below the connecting rod. The roller is rotatably connected to the connecting frame and is located in the placement box.

[0012] The utility model provides a stem cell culture perfusion device. When it is used, a culture bottle is placed in the placement member, the mounting fan fixes the electromagnetic valve below the output pipe, the nutrient box discharges the nutrient solution through the output pipe, the electromagnetic valve is opened, and the nutrient solution flows into the culture bottle, the driving member drives the placement member to rotate, so as to facilitate the perfusion operation of multiple culture bottles in turn, and the opening and closing member is opened to facilitate the removal of the culture bottle. This method can effectively solve the problem that the mouth of the culture bottle is in an open state for a long time during perfusion and it is inconvenient to take out the culture bottle. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0014] Figure 1 It is a structural schematic diagram of the first embodiment of the utility model.

[0015] Figure 2 It is a structural sectional view of the first embodiment of the utility model.

[0016] Figure 3 It is a partial structural schematic diagram of the first embodiment of the utility model.

[0017] Figure 4 It is a structural sectional view of the second embodiment of the utility model.

[0018] 101-workbench, 102-nutrient box, 103-output pipe, 104-placement box, 105-upper cover, 106-installation fan, 107-solenoid valve, 108-opening and closing fan, 109-lift buckle, 110-sealing strip, 111-drive motor, 112-drive rod, 113-positioning seat, 114-rotating disk, 115-placement plate, 116-fixed frame, 117-wrapping pad, 118-through groove, 201-connecting rod, 202-connecting frame, 203-roller. DETAILED DESCRIPTION

[0019] Embodiments of the present utility model will be described in detail below. Examples of the embodiments are shown in the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to explain the present utility model, and should not be construed as a limitation to the present utility model.

[0020] The first embodiment of the present application is as follows:

[0021] Please refer to Figures 1 to 3 , wherein Figure 1 is a schematic structural view of the first embodiment of the present utility model, Figure 2 is a structural sectional view of the first embodiment of the present utility model, Figure 3 is a schematic partial structural view of the first embodiment of the present utility model.

[0022] The present utility model provides a stem cell culture perfusion device, including an operation table 101, a nutrient box 102, an output pipe 103, and a perfusion assembly. The perfusion assembly includes a placement box 104, an upper cover 105, an installation fan 106, a solenoid valve 107, an opening and closing member, a driving member, and multiple groups of placement members. The opening and closing member includes an opening and closing fan 108, a lifting buckle 109, and a sealing strip 110. The driving member includes a driving motor 111, a driving rod 112, a positioning seat 113, and a rotating disk 114. Each group of the placement members includes a placement plate 115, a fixed frame 116, and a wrapping pad 117. The foregoing solution solves the problem that when perfusing the outermost culture bottle, the bottle mouth of the innermost culture bottle will be in an open state for a long time, which easily increases the risk of cell infection, and the device is not convenient for taking out the culture bottle.

[0023] According to the present specific embodiment, the nutrient box 102 is fixedly connected to the workbench 101 and is located above the workbench 101. The output pipe 103 is fixedly connected to the nutrient box 102 and is located on the outside of the nutrient box 102. The perfusion assembly includes a placement box 104, an upper cover 105, a mounting fan 106, a solenoid valve 107, an opening and closing member, a driving member and multiple groups of placement members. The placement box 104 is fixedly connected to the workbench 101 and is located on the outside of the nutrient box 102. The upper cover 105 is fixedly connected to the placement box 104 and is located above the placement box 104. The mounting fan 106 is detachably connected to the placement box 104 and is located on the outside of the upper cover 105. The solenoid valve 107 is fixedly connected to the mounting fan 106 and is located below the output pipe 103. The opening and closing member is hinged to the upper cover 105 and is located on the outside of the upper cover 105. The driving member is hinged to the placement box 104. The placement box 104 is fixedly connected and located in the placement box 104. Multiple groups of the placement parts are fixedly connected to the driving parts and are located above the driving parts. The culture bottles are placed in the placement parts. The placement box 104 reduces the contact between external air and the culture bottles. The upper cover 105 seals the culture bottles. The mounting fan 106 fixes the solenoid valve 107 below the output pipe 103. The output pipe 103 has a valve for controlling the discharge of the nutrient solution in the nutrient box 102. The nutrient box 102 discharges the nutrient solution through the output pipe 103. The solenoid valve 107 is opened to allow the nutrient solution to flow into the culture bottles below the upper cover 105. The driving part drives the placement parts to rotate, which is convenient for taking turns to perfuse multiple culture bottles. Opening the opening and closing parts is convenient for taking out the culture bottles. This method can effectively solve the problem that the mouth of the culture bottle is in an open state for a long time during perfusion and it is inconvenient to take out the culture bottle.

[0024] Among them, the opening and closing fan 108 is hinged to the upper cover 105 and is located on the outside of the upper cover 105, the lifting buckle 109 is fixedly connected to the opening and closing fan 108 and is located above the opening and closing fan 108, and the sealing strip 110 is fixedly connected to the opening and closing fan 108 and is located above the placement box 104. The opening and closing fan 108 is opened by the lifting buckle 109, which makes it convenient to take out the culture bottle or place it under the opening and closing fan 108. After the operation is completed, the opening and closing fan 108 is closed, and the sealing strip 110 improves the airtightness of the opening and closing fan 108.

[0025] Secondly, the output end of the drive motor 111 is fixedly connected to one end of the drive rod 112 and is located inside the placement box 104. The other end of the drive rod 112 is rotatably connected to the positioning seat 113 and is located above the drive motor 111. The positioning seat 113 is fixedly connected to the upper cover 105 and is located below the upper cover 105. The rotating disk 114 is fixedly connected to the drive rod 112 and is located outside the drive rod 112, and the drive rod 112 penetrates through the rotating disk 114. The drive motor 111 drives the drive rod 112 to rotate, the drive rod 112 rotates within the positioning seat 113, and the positioning seat 113 prevents the drive rod 112 from shifting during rotation. The drive rod 112 drives the rotating disk 114 to rotate.

[0026] Meanwhile, the placement plate 115 is fixedly connected to the rotating disk 114 and is located above the rotating disk 114. The fixed frame 116 is fixedly connected to the placement plate 115 and is located above the placement plate 115. The wrapping pad 117 is fixedly connected to the fixed frame 116 and is located inside the fixed frame 116. The placement plate 115 fixes the fixed frame 116. The culture flask is placed within the wrapping pad 117 to enhance the protection of the culture flask, and the culture flask is fixed by the fixed frame 116.

[0027] In addition, the upper cover 105 has a through slot 118 adapted to the mounting fan 106, and the mounting fan 106 is installed within the through slot 118, facilitating the maintenance and replacement of the solenoid valve 107.

[0028] When using a stem cell culture perfusion device of this embodiment, by providing the nutrient box 102, the output pipe 103, and the perfusion assembly, during specific use, the opening and closing fan 108 is opened through the lifting buckle 109, the culture flask is placed within the wrapping pad 117, the fixed frame 116 fixes the culture flask, the mounting fan 106 fixes the solenoid valve 107 below the output pipe 103, the nutrient box 102 exports the nutrient solution through the output pipe 103, the solenoid valve 107 is opened, enabling the nutrient solution to flow into the culture flask below the upper cover 105. After perfusion is completed, it stops. The drive motor 111 drives the drive rod 112 to rotate, the drive rod 112 drives the rotating disk 114 to rotate, and the rotating disk 114 drives the placement plate 115 to rotate, thereby rotating another culture flask to below the solenoid valve 107, facilitating continuous perfusion operations for multiple culture flasks. In this way, only when perfusion operations are performed on the culture flask, the bottle mouth is in an open state, effectively reducing the risk of cell infection, and facilitating the placement and removal of the culture flask, enhancing the convenience and practicality of device use.

[0029] The second embodiment of the present application is as follows:

[0030] Based on the first embodiment, please refer to Figure 4 , Figure 4 which is a structural cross-sectional view of the second embodiment of the present utility model.

[0031] The present utility model provides a stem cell culture perfusion device which further includes four groups of auxiliary components. The four groups of auxiliary components are circumferentially distributed along the axial center line of the rotating disk 114. Each group of auxiliary components includes a connecting rod 201, a connecting frame 202, and a roller 203.

[0032] For this specific embodiment, the connecting rod 201 is fixedly connected to the rotating disk 114 and is located below the rotating disk 114. The connecting frame 202 is fixedly connected to the connecting rod 201 and is located below the connecting rod 201. The roller 203 is rotatably connected to the connecting frame 202 and is located inside the placement box 104. When the rotating disk 114 rotates, the connecting rod 201 drives the connecting frame 202 to move, and the roller 203 rolls inside the placement box 104.

[0033] When using the stem cell culture perfusion device of this embodiment, by setting four groups of auxiliary components, during specific use, when the rotating disk 114 rotates, the connecting rod 201 drives the connecting frame 202 to move, and the roller 203 rolls inside the placement box 104. In this way, it can effectively assist the rotation of the rotating disk 114, improve the smoothness of the device during use, and support the rotating disk 114 to improve the stability of the device.

[0034] The above-disclosed is only a preferred embodiment of the present utility model. Of course, it cannot be used to limit the scope of rights of the present utility model. Those of ordinary skill in the art can understand the entire or part of the process of implementing the above embodiment, and the equivalent changes made according to the claims of the present utility model still fall within the scope covered by the utility model.

Claims

1. A stem cell culture perfusion device, comprising a workbench, characterized in that: It also includes a nutrient box, an output pipe and a perfusion assembly. The nutrient box is fixedly connected to the workbench and is located above the workbench. The output pipe is fixedly connected to the nutrient box and is located outside the nutrient box. The perfusion assembly includes a placement box, an upper cover, a mounting fan, a solenoid valve, an opening and closing member, a driving member and multiple groups of placement members. The placement box is fixedly connected to the workbench and is located outside the nutrient box. The upper cover is fixedly connected to the placement box and is located above the placement box. The mounting fan is detachably connected to the placement box and is located outside the upper cover. The solenoid valve is fixedly connected to the mounting fan and is located below the output pipe. The opening and closing member is hinged to the upper cover and is located outside the upper cover. The driving member is fixedly connected to the placement box and is located inside the placement box. Multiple groups of placement members are fixedly connected to the driving member and are located above the driving member.

2. The stem cell culture perfusion device according to claim 1, characterized in that: The opening and closing member includes an opening and closing fan, a lifting buckle and a sealing strip. The opening and closing fan is hinged to the upper cover and is located on the outer side of the upper cover. The lifting buckle is fixedly connected to the opening and closing fan and is located above the opening and closing fan. The sealing strip is fixedly connected to the opening and closing fan and is located above the placement box.

3. The stem cell culture perfusion device according to claim 2, characterized in that: The driving member includes a driving motor, a driving rod, a positioning seat and a rotating disk. The output end of the driving motor is fixedly connected to one end of the driving rod and is located in the placement box. The other end of the driving rod is rotatably connected to the positioning seat and is located above the driving motor. The positioning seat is fixedly connected to the upper cover and is located below the upper cover. The rotating disk is fixedly connected to the driving rod and is located on the outside of the driving rod, and the driving rod passes through the rotating disk.

4. The stem cell culture perfusion device according to claim 3, characterized in that: Each group of the placing parts includes a placing plate, a fixed frame and a wrapping pad. The placing plate is fixedly connected to the rotating disk and is located above the rotating disk. The fixed frame is fixedly connected to the placing plate and is located above the placing plate. The wrapping pad is fixedly connected to the fixed frame and is located inside the fixed frame.

5. The stem cell culture perfusion device according to claim 4, characterized in that: The upper cover has a through slot adapted to the mounting fan.

6. The stem cell culture perfusion device according to claim 5, characterized in that: The stem cell culture perfusion device also includes four groups of auxiliary components, which are distributed circumferentially along the axial center line of the rotating disk. Each group of auxiliary components includes a connecting rod, a connecting frame and a roller. The connecting rod is fixedly connected to the rotating disk and is located below the rotating disk. The connecting frame is fixedly connected to the connecting rod and is located below the connecting rod. The roller is rotatably connected to the connecting frame and is located in the placement box.

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