Cell resuscitator based on dry-type anti-pollution metal bath

Abstract

The utility model provides a cell resuscitation instrument based on a dry-type anti-pollution metal bath. The cell resuscitation instrument comprises a resuscitation instrument body, a bearing groove is formed in the resuscitator body; a driving motor and a heating device are mounted at the groove bottom of the bearing groove; a rotating tray is arranged at the output end of the driving motor; the rotary tray is used for bearing and rotating the heat conduction cup; the heating device is used for heating the heat conduction cup; a metal heat conduction ball is arranged in the heat conduction cup and is used for wrapping a cell cryopreservation tube or a cell cryopreservation bag so as to heat and resuscitate cells in the cell cryopreservation tube or the cell cryopreservation bag. According to the utility model, the cells in the cell cryopreservation tube or the cell cryopreservation bag can be heated and resuscitated, so that the pollution to the cells in the cell cryopreservation tube or the cell cryopreservation bag is avoided, and clean and dry resuscitation is realized.

Description

Technical Field

[0001] The utility model relates to a high-throughput cell resuscitation device, and particularly to a cell resuscitator based on a dry anti-pollution metal bath. Background Technique

[0002] With the development of life science and technology, cell therapy technology is advancing rapidly, mainly including stem cell therapy and immune cell therapy represented by CAR-T cells. The preparation process of cell products involves cell cryopreservation and thawing. When thawing cells, the best operation is to quickly warm the cell cryopreservation bag or cell cryopreservation tube just taken out from the liquid nitrogen tank to 37°C, so that it quickly passes through the -5°C to 0°C range where cells are most vulnerable to damage, to avoid damage to cells caused by recrystallization of ice crystals and resulting in cell death.

[0003] Common cell cryopreservation containers are cell cryopreservation tubes and cell cryopreservation bags. The cryopreservation capacity of cell cryopreservation tubes is mostly less than 5 ml, and for cell cryopreservation requirements exceeding 5 ml, cell cryopreservation bags are mostly used. Cell cryopreservation bags are more suitable for commercial applications of stem cells and immune cells, such as CAR-T cell therapy. Currently, the thawing of cell cryopreservation bags can adopt the water bath thawing method or the dry thawing method. The former water bath thawing method requires full manual operation, cannot implement standardized operation, and is prone to cell contamination, and has gradually been phased out. The latter dry thawing method currently has limited products, a single thawing method, and the thawing efficiency is not yet satisfactory, and there is still a great need for improvement and development.

[0004] Currently, both cell cryopreservation tubes and cell cryopreservation bags are widely used, but there is no product that can simultaneously meet the anhydrous dry thawing of cell cryopreservation bags and high-throughput cell cryopreservation tubes, nor is there a product that can simultaneously meet the safe and convenient transportation problems of cell cryopreservation bags and cell cryopreservation tubes. To solve the aforementioned problems, the present utility model comes into being.

[0005] When retrieving the prior art, in the patent "A Dry Resuscitation Device for Cell Freezing Bags" applied by the applicant, with the application number 201910119077.3, there are still areas for further optimization and improvement in the actual use of this cell resuscitation device. For example, the product lacks universality and can only warm up and resuscitate cell freezing bags, but not cell freezing tubes; after the cell freezing bag is rewarmed, it cannot perform rapid response cooling treatment, which may affect the cell resuscitation effect in the high-temperature resuscitation mode; due to the presence of the temperature measurement probe on the heating tray, some heating area has to be sacrificed, affecting the heating efficiency of the tray; the temperature measurement probe can only measure the temperature at one point and cannot monitor the overall temperature change of the cell freezing bag, which is prone to misjudgment and affects the cell resuscitation effect; the heating tray is in direct contact with the cell freezing bag to be resuscitated, and there may be an air gap, which affects heat conduction; only the problem of anhydrous dry resuscitation of cell freezing bags is considered, and the problem of safe, efficient, and convenient short-distance and short-time transportation requirements during the process of transporting cell freezing bags or cell freezing tubes from low-temperature storage facilities to the cell resuscitation operation table is not considered. Utility Model Content

[0006] Aiming at the deficiencies in the prior art, the purpose of the present utility model is to provide a cell resuscitator based on a dry anti-pollution metal bath.

[0007] The cell resuscitator based on a dry anti-pollution metal bath provided by the present utility model includes: a resuscitator body;

[0008] A carrying groove is provided on the resuscitator body; a driving motor and a heating device are installed at the bottom of the carrying groove;

[0009] A rotating tray is provided at the output end of the driving motor;

[0010] The rotating tray is used to carry and rotate a heat conduction cup;

[0011] The heating device is used for heating the heat conduction cup;

[0012] A metal heat conduction ball is arranged in the heat conduction cup, and the metal heat conduction ball is used to wrap the cell freezing tube or the cell freezing bag to heat and resuscitate the cells in the cell freezing tube or the cell freezing bag.

[0013] Preferably, the metal heat conduction ball is made of aluminum, copper or alloy.

[0014] Preferably, the metal heat conduction ball is made of aerospace aluminum alloy.

[0015] Preferably, the diameter of the metal heat conduction ball is 100 microns to 5000 microns.

[0016] Preferably, the heating device adopts an air flow rotary heating device.

[0017] Preferably, the air flow rotation heating device includes: a heating air flow generating device and a cooling air flow generating device;

[0018] The heating air flow generating device is used for heating and raising the temperature of the heat-conducting cup;

[0019] The cooling air flow generating device is used for cooling the heat-conducting cup;

[0020] An air flow through hole is provided at the bottom of the bearing groove.

[0021] Preferably, the heat-conducting cup is made of aluminum, copper or an alloy.

[0022] Preferably, the heat-conducting cup is arranged in the bearing groove;

[0023] A clamping block for clamping the heat-conducting cup is provided on the groove wall of the bearing groove.

[0024] Preferably, an interaction panel is provided on the resuscitator body;

[0025] The interaction panel is used for controlling the heating device and the driving motor.

[0026] Preferably, a limiting member is provided on the outer side wall of the heat-conducting cup, and the limiting member is used for limiting the depth of the heat-conducting cup inserted into the bearing groove.

[0027] Preferably, an ice tray box is provided on the resuscitator body, and tube holes arranged in a matrix are provided on the ice tray box.

[0028] Compared with the prior art, the utility model has the following beneficial effects:

[0029] A bearing groove is provided on the resuscitator body of the utility model. A driving motor and a heating device are installed at the bottom of the bearing groove. A rotating tray is arranged at the output end of the driving motor. The heat-conducting cup is carried and rotated by the rotating tray. The heat-conducting cup is heated by the heating device. A metal heat-conducting ball is arranged in the heat-conducting cup. The cell cryopreservation tube or the cell cryopreservation bag is wrapped by the metal heat-conducting ball, so that the cells in the cell cryopreservation tube or the cell cryopreservation bag can be heated and resuscitated, the pollution of the cells in the cell cryopreservation tube or the cell cryopreservation bag is avoided, and clean and dry resuscitation is realized. Description of the Drawings

[0030] By reading the detailed description of the non-restrictive embodiments with reference to the following drawings, other features, purposes and advantages of the utility model will become more obvious:

[0031] Figure 1Schematic diagram of the structure of a cell resuscitator based on a dry anti-pollution metal bath in an embodiment of the present utility model;

[0032] Figure 2 Schematic diagram of the working state of a cell resuscitator based on a dry anti-pollution metal bath in an embodiment of the present utility model;

[0033] Figure 3 Schematic diagram of the structure of a cell resuscitator based on a dry anti-pollution metal bath in a variant embodiment of the present utility model;

[0034] Figure 4 Schematic diagram of the structure of a cell resuscitator based on a dry anti-pollution metal bath in another variant embodiment of the present utility model;

[0035] Figure 5 First schematic diagram of the structure of an ice tray box in a variant embodiment of the present utility model;

[0036] Figure 6 First cross-sectional schematic diagram of the structure of an ice tray box in a variant embodiment of the present utility model;

[0037] Figure 7 Second schematic diagram of the structure of an ice tray box in a variant embodiment of the present utility model;

[0038] Figure 8 Third schematic diagram of the structure of an ice tray box in a variant embodiment of the present utility model;

[0039] Figure 9 Fourth schematic diagram of the structure of an ice tray box in a variant embodiment of the present utility model;

[0040] Figure 10 Fifth schematic diagram of the structure of an ice tray box in a variant embodiment of the present utility model;

[0041] Figure 11 Schematic diagram of the structure of a cell resuscitator based on a dry anti-pollution metal bath in still another variant embodiment of the present utility model;

[0042] Figure 12 Schematic diagram of the structure of a heat-conducting cup in still another variant embodiment of the present utility model; and

[0043] Figure 13 Comparison chart of cell resuscitation mode survival rates in an embodiment of the present utility model.

[0044] In the figure:

[0045] 1 is the resuscitator body; 2 is the bearing groove; 3 is the interaction panel; 4 is the heat-conducting cup; 5 is the metal heat-conducting ball; 6 is the cell cryopreservation tube; 7 is the ice tray box; 701 is the tube hole. Detailed implementation manners

[0046] The present utility model will be described in detail below in conjunction with specific embodiments. The following embodiments will help those skilled in the art to further understand the present utility model, but do not limit the present utility model in any form. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present utility model, several deformations and improvements can still be made. These all belong to the protection scope of the present utility model.

[0047] Figure 1 As shown in the structural schematic diagram of the cell resuscitator based on a dry anti-pollution metal bath in the embodiment of the present utility model, Figure 1 as shown, the cell resuscitator based on a dry anti-pollution metal bath provided by the present utility model includes: a resuscitator body 1;

[0048] A bearing groove 2 is provided on the resuscitator body 1; a driving motor and a heating device are installed at the bottom of the bearing groove 2;

[0049] The output end of the driving motor is provided with a rotating tray for driving the rotating tray to rotate and shake;

[0050] The rotating tray is used for carrying and rotating a heat-conducting cup 4;

[0051] The heating device is used for heating the heat-conducting cup 4;

[0052] Figure 2 As shown in the working state schematic diagram of the cell resuscitator based on a dry anti-pollution metal bath in the embodiment of the present utility model, Figure 2 as shown, a metal heat-conducting ball 5 is arranged in the heat-conducting cup 4, and the metal heat-conducting ball 5 is used for wrapping a cell cryopreservation tube 6 or a cell cryopreservation bag to heat and resuscitate the cells in the cell cryopreservation tube 6 or the cell cryopreservation bag.

[0053] In an embodiment of the present utility model, the metal heat-conducting ball 5 is made of aluminum, copper or an alloy.

[0054] In an embodiment of the present utility model, the metal heat-conducting ball 5 is made of aerospace aluminum alloy.

[0055] In an embodiment of the present utility model, the diameter of the metal heat-conducting ball 5 is 100 microns to 5000 microns.

[0056] In an embodiment of the present utility model, the heating device adopts an air-flow rotation heating device. The air-flow rotation heating device includes: a heating air-flow generating device and a cooling air-flow generating device;

[0057] The heating air-flow generating device is used for heating and raising the temperature of the heat-conducting cup 4;

[0058] The cooling air-flow generating device is used for cooling the heat-conducting cup 4;

[0059] An air circulation hole is provided at the bottom of the bearing groove 2.

[0060] In an embodiment of the present utility model, the heat-conducting cup 4 is arranged in the bearing groove 2; a limiting member is arranged on the outer side wall of the heat-conducting cup, and the limiting member is used to limit the depth of the heat-conducting cup inserted into the bearing groove;

[0061] A clamping block for clamping the heat-conducting cup 4 is arranged on the groove wall of the bearing groove 2.

[0062] The heat-conducting cup 4 is made of aluminum, copper or alloy.

[0063] In an embodiment of the present utility model, an interaction panel 3 is arranged on the resuscitator body 1;

[0064] The interaction panel 3 is used to control the heating device and the driving motor.

[0065] When using the cell resuscitator based on the dry anti-pollution metal bath provided by the present utility model, adjust the resuscitation temperature to 37°C through the interaction panel 3 and preheat for 10 minutes until the micron-level aviation heat-conducting aluminum alloy particles are also at 37°C, then put in the cell cryopreservation tube 6 or the cell cryopreservation bag, and press the resuscitation button to achieve one-key resuscitation of the cells. It can also be directly resuscitated at the Pasteur disinfection temperature of 60°C. One-key resuscitation can be used in the first minute, and then take it out to check every once in a while. Once it is found that the volume of the ice block is less than 1 cm, take it out directly and shake it by hand until the ice block disappears. As long as there is ice in the tube, the temperature in the tube is 0°C of the ice-water mixture, and there is no need to worry that the cells will be scalded to death at 60°C.

[0066] Figure 3 It is a schematic structural diagram of the cell resuscitator based on the dry anti-pollution metal bath in the variant of the present utility model, as Figure 3 shown, an ice tray box 7 is arranged on one side of the resuscitator body 1, and tube holes 701 arranged in a matrix are arranged on the ice tray box 7.

[0067] The ice tray box 7 is arranged adjacent to the bearing groove 2. The tube holes 701 can be used to place metal heat-conducting balls 5 or test tubes. Figure 6 It is a schematic cross-sectional view of the first structure of the ice tray box in the variant of the present utility model, as Figure 6 shown, a heat preservation liquid can be placed in the gap between adjacent tube holes 701 to realize flexible placement of various test tubes in the tube holes 701 in the range of 2°C to 100°C.

[0068] Figure 4 It is a schematic structural diagram of the cell resuscitator based on the dry anti-pollution metal bath in the variant of the present utility model, as Figure 4As shown, an ice tray box receiving groove is provided on the other side of the resuscitator body 1. The ice tray box receiving groove is used to place the ice tray box 7 and can also accommodate the metal heat conduction balls 5.

[0069] Figure 5 This is the first structural schematic diagram of the ice tray box in the variant of the present utility model. Figure 7 This is the second structural schematic diagram of the ice tray box in the variant of the present utility model. Figure 8 This is the third structural schematic diagram of the ice tray box in the variant of the present utility model. Figure 9 This is the fourth structural schematic diagram of the ice tray box in the variant of the present utility model. Figure 10 This is the fifth structural schematic diagram of the ice tray box in the variant of the present utility model. As Figure 5 shown, the ice tray box is provided with 4 50-ml centrifuge tube holes, 6 15-ml centrifuge tube holes, 36 2-ml centrifuge tube cell cryopreservation tube holes, 12 1.5- or 2-ml centrifuge tube holes, 96 0.2- or 0.5-ml centrifuge tubes, one 0.1- or 0.2-ml PCR plate hole or 12 PCR eight-tube strips; as Figures 7 to 10 shown, the tube holes can be provided with different numbers and different sizes to adapt to different test tubes.

[0070] Figure 11 This is the structural schematic diagram of the cell resuscitator based on a dry-type anti-pollution metal bath in another variant of the present utility model. Figure 12 This is the structural schematic diagram of the heat conduction cup in another variant of the present utility model. As Figure 11 、 Figure 12 shown, the heat conduction cup 4 is columnar, and is provided with a plurality of cryopreservation tube holes, such as 3, which can be used for the placement and resuscitation of cryopreservation tubes.

[0071] Figure 13 This is the comparison chart of the survival rate of the cell resuscitation mode in the embodiment of the present utility model. As Figure 13 shown, by comparing the present utility model with an ordinary water bath for various cells from the Cell Bank of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, the survival rates of the cell resuscitator based on a dry-type anti-pollution metal bath in the present utility model in the 37° normal gear and 60° fast gear are significantly higher than those of the ordinary water bath mode.

[0072] In the present utility model, a carrying groove is provided on the resuscitator body. A driving motor and a heating device are installed at the bottom of the carrying groove. The output end of the driving motor is provided with a rotating tray. The heat conduction cup is carried and rotated by the rotating tray. The heat conduction cup is heated by the heating device. Metal heat conduction balls are arranged in the heat conduction cup. By wrapping the cell cryopreservation tube or cell cryopreservation bag with the metal heat conduction balls, the cells in the cell cryopreservation tube or cell cryopreservation bag can be heated and resuscitated, avoiding the pollution of the cells in the cell cryopreservation tube or cell cryopreservation bag, and realizing clean and dry resuscitation.

[0073] The specific embodiments of the present utility model have been described above. It should be understood that the present utility model is not limited to the above specific embodiments, and those skilled in the art can make various deformations or modifications within the scope of the claims, which do not affect the essence of the present utility model.

Claims

1. A cell recovery instrument based on a dry anti-pollution metal bath, characterized in that: include: Resuscitation device body; The resuscitation instrument body is provided with a bearing slot; a driving motor and a heating device are installed at the bottom of the bearing slot; The output end of the driving motor is provided with a rotating tray; The rotating tray is used to carry and rotate the thermal cup; The heating device is used to heat the thermally conductive cup; A metal heat-conducting ball is arranged in the heat-conducting cup, and the metal heat-conducting ball is used to wrap the cell freezing tube or the cell freezing bag to heat and revive the cells in the cell freezing tube or the cell freezing bag.

2. The cell recovery instrument based on dry anti-pollution metal bath according to claim 1, characterized in that: The metal heat-conducting ball is made of aluminum, copper or alloy.

3. The cell recovery instrument based on dry anti-pollution metal bath according to claim 1, characterized in that: The metal heat-conducting ball is made of aviation aluminum alloy.

4. The cell recovery instrument based on dry anti-pollution metal bath according to claim 1, characterized in that: The diameter of the metal heat-conducting ball is 100 microns to 5000 microns.

5. The cell recovery instrument based on dry anti-pollution metal bath according to claim 1, characterized in that: The heating device is an airflow rotating heating device.

6. The cell recovery instrument based on dry anti-pollution metal bath according to claim 5, characterized in that: The airflow rotating heating device comprises: a heating airflow generating device and a cooling airflow generating device; The heating airflow generating device is used to heat the thermal cup; The cooling airflow generating device is used to cool the thermal cup; The bottom of the bearing groove is provided with air flow holes.

7. The cell recovery instrument based on dry anti-pollution metal bath according to claim 1, characterized in that: The heat-conducting cup is made of aluminum, copper or alloy.

8. The cell recovery instrument based on dry anti-pollution metal bath according to claim 1, characterized in that: The heat conducting cup is arranged in the bearing groove; A clamping block for clamping the heat-conducting cup is arranged on the groove wall of the bearing groove.

9. The cell recovery instrument based on dry anti-pollution metal bath according to claim 1, characterized in that: An interactive panel is provided on the resuscitation instrument body; The interactive panel is used to control the heating device and the driving motor.

10. The cell recovery instrument based on dry anti-pollution metal bath according to claim 1, characterized in that: The resuscitation instrument body is provided with an ice tray box, and the ice tray box is provided with tube holes arranged in a matrix.

Images