Flow fluorescent liquid path system

Abstract

The utility model relates to the field of biological detection, in particular to a liquid path system for flow fluorescence, which comprises a flow chamber component, a sheath flow system, a sample loading system, a cleaning system and a waste discharge system, the flow chamber assembly comprises a flow chamber, a rectifying cavity and a sample injection needle which are communicated in sequence, a first connecting port and a second connecting port are formed in the side face of the rectifying cavity, a waste liquid outlet is formed in the top of the flow chamber, the first connecting port is connected with the sheath flow system, the second connecting port is connected with the cleaning system, and the sample injection needle is connected with the sample loading system. And the waste liquid outlet is connected with a waste discharge system. According to the liquid path system, the function of completely emptying liquid in the flowing chamber in the cleaning process can be achieved, in this way, even if large-particle impurities exist in the flowing chamber, the large-particle impurities can be discharged, the flowing chamber is prevented from being blocked, and in the instrument storage process, due to the fact that no liquid exists in the flowing chamber, mould breeding can be restrained, and the flowing chamber can be clean for a longer time.

Description

Technical Field

[0001] The utility model relates to the field of biological detection, in particular to a liquid path system for flow-through fluorescence luminescence immunoassay analysis. Background Art

[0002] Flow-through fluorescence detection technology, also known as liquid-phase chip technology or microsphere suspension chip, is a new type of biochip technology based on the xMAP technology developed by Luminex Corporation in the United States, and is a new generation of high-throughput diagnostic technology platform following chemiluminescence technology. The liquid-phase chip system is composed of many uniformly sized circular microspheres (diameter 4-8 um), and each microsphere corresponds to a separate fluorescence code. The instrument is used to detect and analyze the microspheres. Flow-through fluorescence products integrate fluorescence-coded microsphere technology, laser analysis technology, flow cytometry technology, high-speed digital signal processing technology, and data processing algorithms, and have the advantages of free combination, high throughput, high speed, low cost, high accuracy, good repeatability, high sensitivity, wide linear range, simple operation, and the ability to complete multiple project detections on the same platform, representing the development direction of basic life science research and medical diagnostic technology, and is one of the trend technologies in clinical diagnosis.

[0003] Flow-through fluorescence luminescence immunoassay analysis requires a liquid path system to arrange the coded microspheres in a single column under the wrapping of sheath fluid and pass through the flow cell at a uniform speed in sequence. The center of the flow cell is a laser beam after shaping. When the coded microspheres pass through the laser beam, scattered light is generated by the microsphere size, and fluorescence is generated by the excitation of the fluorescent dye. The scattered light is collected and detected in the forward direction, and the fluorescence enters the semiconductor micro-light detection module after being collected, split by the optical system.

[0004] The flow-through fluorescence liquid path system is one of the core systems of a flow cytometer, and the stability and automation degree of the liquid path system have an important impact on the overall test and throughput.

[0005] The existing system has the following defects or deficiencies:

[0006] 1. The cleaning is not thorough enough. If there are large-diameter impurities in the cleaning liquid, it is easy to cause blockage of the flow cell. At the same time, if the inside of the flow cell cavity has been filled with liquid from the production of the instrument to the time when the user finally starts to use it, there may be a risk of long-term bacteria contamination on the surface of the detection chamber;

[0007] 2. In traditional cleaning, the internal cavity of the flow cell is filled with sheath fluid before cleaning. Then, a cleaning mechanism is used to perfusion-cleaning fluid. During this process, the cleaning fluid will be diluted by the sheath fluid and flow through the flushing flow cell, thereby achieving the purpose of cleaning. Finally, sheath fluid is perfused into the flow cell again to dilute and replace the internal liquid of the flow cell with sheath fluid. However, it takes a long time to completely replace it with sheath fluid. If the perfusion volume of the sheath fluid is insufficient during this process, bubbles may be generated during the sample loading process due to the influence of the cleaning fluid, which will affect the formation of the sheath flow and lead to test failure;

[0008] 3. During the sample loading and detection process, there is no internal pressure monitoring of the flow cell or the pressure compensation function cannot be realized before each sample loading test.

[0009] 4. The cost of the liquid path system is relatively high. Summary of the Invention

[0010] In order to solve the above problems existing in the prior art and further improve the degree of automation, the present utility model provides a liquid path system for flow cytometry fluorescence.

[0011] To achieve the above technical effects, the present utility model is realized through the following technical solutions:

[0012] A liquid path system for flow cytometry fluorescence includes a flow cell assembly, a sheath flow system, a sample loading system, a cleaning system, and a waste discharging system; the flow cell assembly includes a flow cell, a rectifying cavity, and a sampling needle that are connected in sequence. A first connection port and a second connection port are respectively arranged on the side of the rectifying cavity. A waste liquid outlet is arranged at the top of the flow cell. Among them, the first connection port is connected to the sheath flow system, the second connection port is connected to the cleaning system, the sampling needle is connected to the sample loading system, and the waste liquid outlet is connected to the waste discharging system through.

[0013] Further, the sheath flow system includes a three-way solenoid valve SV2, a three-way solenoid valve SV9, a peristaltic pump P1, a three-way solenoid valve SV8, a normally closed two-way solenoid valve SV5, a filter F1, and a sheath fluid pressure sensor PS1. The three-way solenoid valve SV2 is respectively connected to a sheath fluid barrel and the three-way solenoid valve SV9. The three-way solenoid valve SV9 is connected to the three-way solenoid valve SV8. A peristaltic pump P1 is connected between the three-way solenoid valve SV9 and the three-way solenoid valve SV8. The three-way solenoid valve SV8 is connected to the liquid inlet of the filter F1. The liquid outlet of the filter F1 is connected to the normally closed two-way solenoid valve SV5. The exhaust port of the filter F1 is connected to the normally closed two-way solenoid valve SV1. The normally closed two-way solenoid valve SV5 is connected to the first connection port. The sheath fluid pressure sensor PS1 is connected to the second connection port. As Figure 3 shown.

[0014] Further, the sample loading system uses a plunger pump P2 for quantitatively sucking and pushing out samples. The sample loading system includes a sampling needle, a first sample loading pipeline, a three-way pinch valve solenoid valve SV6, a second sample loading pipeline, a plunger pump P2, and a third sample loading pipeline. The plunger pump P2 is connected to the three-way solenoid valve SV4. The solenoid valve SV4 is connected to the common end of the three-way pinch valve solenoid valve SV6 through the second sample loading pipeline. The normally closed end of the three-way pinch valve solenoid valve SV6 is connected to the sampling needle of the flow cell assembly through the third sample loading pipeline. The normally open end of the three-way pinch valve solenoid valve SV6 is connected to the sampling needle through the first sample loading pipeline.

[0015] Furthermore, the inner diameter of the first sample loading pipeline is 0.5 mm - 0.8 mm, the inner diameter of the second sample loading pipeline is 0.5 mm - 1.0 mm, and the inner diameter of the third sample loading pipeline is approximately 0.3 mm - 0.8 mm.

[0016] Further, the cleaning system includes the three-way solenoid valve SV2, the three-way solenoid valve SV9, the three-way solenoid valve SV8, and the peristaltic pump P1 shared with the sheath flow system, and also includes the three-way solenoid valve SV3, the cleaning tank W1, and the three-way solenoid valve SV7. The cleaning liquid barrel is connected to the three-way solenoid valve SV2. The three-way solenoid valve SV2 is connected to the three-way solenoid valve SV9. The three-way solenoid valve SV9 is connected to the three-way solenoid valve SV8. And a peristaltic pump P1 is arranged between the three-way solenoid valve SV9 and the three-way solenoid valve SV8. The solenoid valve SV8 is connected to the three-way solenoid valve SV3. The three-way solenoid valve SV3 is respectively connected to the second connection port of the flow cell assembly and the cleaning tank W1. The common end of the three-way solenoid valve SV7 is connected to the waste liquid outlet of the flow cell assembly. It is composed as Figure 5 shown.

[0017] Further, the waste discharging system includes a diaphragm pump P3, a cleaning tank W1, and a normally closed two-way solenoid valve SV10. The waste liquid barrel is connected to the diaphragm pump P3. The diaphragm pump P3 is connected to the cleaning tank W1.

[0018] The beneficial effects of the present utility model are as follows:

[0019] 1. Compared with the comparative patent in terms of the liquid path of the present application, the implementation method of the cleaning system is different. The liquid path system of the present application can achieve the function of completely emptying the liquid inside the flow cell. In this way, even if there are large particle impurities inside the flow cell, they can be discharged, avoiding the blockage of the flow cell. During the storage of the instrument, since there is no liquid inside the flow cell, the growth of mold can be inhibited, keeping the flow cell clean for a longer time.

[0020] 2. Cleaning process of this application: Empty the flow cell -> Clean the flow cell with cleaning solution -> Soak -> Empty the flow cell -> Clean the flow cell with sheath fluid -> Empty the flow cell -> Fill the flow cell with sheath fluid. After emptying the flow cell, when using "clean the flow cell with cleaning solution", there will be no problem of the cleaning solution being diluted, so the cleaning effect is better. Executing the "empty the flow cell" instruction again before "fill the flow cell with sheath fluid" can reduce the influence caused by the residue of the cleaning solution in the sheath fluid.

[0021] 3. The power pump of the cleaning system of this application shares the power pump of the sheath flow system. Through the switching combination of different valves, various functions such as sample loading, cleaning, and filling can be achieved. There are fewer components, the liquid path system is stable, and the overall cost is low. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the overall structure of this application.

[0023] Figure 2 It is a schematic diagram of the flow cell assembly.

[0024] Figure 3 It is a schematic diagram of the structure of the sheath flow system.

[0025] Figure 4 It is a schematic diagram of the structure of the sample loading system.

[0026] Figure 5 It is a schematic diagram of the structure of the cleaning system.

[0027] Figure 6 It is a schematic diagram of the structure of the waste discharge system.

[0028] In the drawings:

[0029] 10 - Flow cell assembly, 20 - Sheath flow system, 30 - Sample loading system, 40 - Cleaning system, 50 - Waste discharge system, 100 - Flow cell, 101 - Rectifying cavity, 102 - Sampling needle, 103 - First connection port, 104 - Second connection port, 105 - Waste liquid outlet, 300 - Sampling needle, 301 - First sample loading pipeline, 302 - Second sample loading pipeline, 303 - Third sample loading pipeline. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0030] The following provides a detailed description of the embodiments of the present invention. These embodiments are implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operation processes are given. However, the protection scope of the present invention is not limited to the following embodiments.

[0031] It should be noted that all the directional indications in the embodiments of the present invention (such as both sides, edges, upper, lower, left, right, front, rear, middle, top end, bottom end, tail, axial direction, radial direction... are only used to explain the relative positional relationship and motion state between various components in a specific posture (as shown in the attached drawings). If this specific posture changes, the directional indications will also change accordingly.

[0032] Embodiment 1

[0033] As Figure 1 and Figure 2 shown, a liquid path system for flow cytometry includes a flow cell assembly 10, a sheath flow system 20, a sampling system 30, a cleaning system 40, and a waste discharge system 50; the flow cell assembly 10 includes a flow cell 100, a rectifying cavity 101, and a sampling needle 102 that are connected in sequence. A first connection port 103 and a second connection port 104 are respectively arranged on the side surface of the rectifying cavity 101. A waste liquid outlet 105 is arranged at the top of the flow cell 100. Among them, the first connection port 103 is connected to the sheath flow system 20, the second connection port 104 is connected to the cleaning system 40, the sampling needle 102 is connected to the sampling system 30, and the waste liquid outlet 105 is connected to the waste discharge system 50 through.

[0034] Compared with the comparative patent, the implementation method of the cleaning system 40 in the liquid path of the present application is different. The liquid path system of the present application can realize the function of completely emptying the liquid inside the flow cell assembly 10 during the cleaning process. In this way, even if there are large particle impurities inside the flow cell assembly 10, they can be discharged to avoid clogging of the flow cell 100. During the storage of the instrument, since there is no liquid inside the flow cell assembly 10, the growth of mold can be inhibited, keeping the flow cell 100 clean for a longer time.

[0035] Embodiment 2

[0036] As Figure 1 and Figure 2 shown, a liquid path system for flow cytometry includes a flow cell assembly 10, a sheath flow system 20, a sampling system 30, a cleaning system 40, and a waste discharge system 50; the flow cell assembly 10 includes a flow cell 100, a rectifying cavity 101, and a sampling needle 102 that are connected in sequence. A first connection port 103 and a second connection port 104 are respectively arranged on the side surface of the rectifying cavity 101. A waste liquid outlet 105 is arranged at the top of the flow cell 100. Among them, the first connection port 103 is connected to the sheath flow system 20, the second connection port 104 is connected to the cleaning system 40, the sampling needle 102 is connected to the sampling system 30, and the waste liquid outlet 105 is connected to the waste discharge system 50 through.

[0037] The sheath flow system 20 includes a three-way solenoid valve SV2, a three-way solenoid valve SV9, a peristaltic pump P1, a three-way solenoid valve SV8, a normally-closed two-way solenoid valve SV5, a filter F1, and a sheath fluid pressure sensor PS1. The three-way solenoid valve SV2 is respectively connected to the sheath fluid barrel and the three-way solenoid valve SV9. The three-way solenoid valve SV9 is connected to the three-way solenoid valve SV8. A peristaltic pump P1 is connected between the three-way solenoid valve SV9 and the three-way solenoid valve SV8. The three-way solenoid valve SV8 is connected to the liquid inlet of the filter F1. The liquid outlet of the filter F1 is connected to the normally-closed two-way solenoid valve SV5. The exhaust port of the filter F1 is connected to the normally-closed two-way solenoid valve SV1. The normally-closed two-way solenoid valve SV5 is connected to the first connection port 103. The sheath fluid pressure sensor PS1 is connected to the second connection port 104, as Figure 3 shown.

[0038] Sheath flow formation process: The three-way solenoid valves SV2 and SV9 are normally open and conducting. The normally-closed end of the three-way solenoid valve SV8 is conducting. The normally-closed two-way solenoid valve SV5 is opened. After a delay of 200 ms, the peristaltic pump P1 rotates at the sheath flow formation speed. After a certain period of time, according to the feedback of the pressure value of the sheath fluid pressure sensor PS1, the rotation speed of the peristaltic pump P1 is automatically adjusted to increase or decrease. After the pressure of the peristaltic pump PS1 meets the requirements, it rotates at a constant speed again at the sheath flow formation speed. The sheath flow passes through the three-way solenoid valve SV2, the three-way solenoid valve SV9, the peristaltic pump P1, the three-way solenoid valve SV8, the filter F1, and the normally-closed two-way solenoid valve SV5 in sequence from the sheath fluid barrel, and finally enters the flow cell assembly 10 to form the required sheath flow.

[0039] The sample loading system 30 uses a plunger pump P2 for quantitative aspiration and ejection of the sample. The sample loading system 30 includes a sampling needle 300, a first sample loading pipeline 301, a three-way pinch valve solenoid valve SV6, a second sample loading pipeline 302, a plunger pump P2, and a third sample loading pipeline 303. The plunger pump P2 is connected to the three-way solenoid valve SV4. The solenoid valve SV4 is connected to the common end of the three-way pinch valve solenoid valve SV6 through the second sample loading pipeline 302. The normally-closed end of the three-way pinch valve solenoid valve SV6 is connected to the sampling needle 102 of the flow cell assembly 10 through the third sample loading pipeline 303. The normally-open end of the three-way pinch valve solenoid valve SV6 is connected to the sampling needle 300 through the first sample loading pipeline 301.

[0040] The inner diameter of the first sample loading pipeline 301 is 0.5 mm - 0.8 mm, the inner diameter of the second sample loading pipeline 302 is 0.5 mm - 1.0 mm, and the inner diameter of the third sample loading pipeline 303 is about 0.3 mm - 0.8 mm.

[0041] When loading samples, the sampling needle 300 first moves into the sample to be tested. At this time, the normally open ends of the three-way solenoid valve SV4 and the three-way pinch valve solenoid valve SV6 are in the conducting state. The plunger pump P2 is turned on to quantitatively aspirate the sample. After the sample is aspirated, the sampling needle 300 is lifted out of the sample. The plunger pump P2 aspirates again, and this time the aspirated is air. When the sample aspiration just exceeds the normally open end joint of the three-way pinch valve solenoid valve SV6, the normally closed end of the three-way pinch valve solenoid valve SV6 is put into the conducting state. The plunger pump P2 pushes the sample into the flow cell assembly 10, and cooperates with the sheath flow system 20 to form a testable sample flow. As Figure 4 shown.

[0042] The cleaning system 40 includes the three-way solenoid valve SV2, the three-way solenoid valve SV9, the three-way solenoid valve SV8, and the peristaltic pump P1 shared with the sheath flow system 20. It also includes the three-way solenoid valve SV3, the cleaning tank W1, and the three-way solenoid valve SV7. The cleaning liquid barrel is connected to the three-way solenoid valve SV2. The three-way solenoid valve SV2 is connected to the three-way solenoid valve SV9. The three-way solenoid valve SV9 is connected to the three-way solenoid valve SV8. And a peristaltic pump P1 is arranged between the three-way solenoid valve SV9 and the three-way solenoid valve SV8. The solenoid valve SV8 is connected to the three-way solenoid valve SV3. The three-way solenoid valve SV3 is respectively connected to the second connection port 104 of the flow cell assembly 10 and the cleaning tank W1. The common end of the three-way solenoid valve SV7 is connected to the waste liquid outlet 105 of the flow cell 100 assembly. The composition is as Figure 5 shown.

[0043] During the cleaning process, the following instructions will be executed: evacuate the flow cell -> clean the flow cell with cleaning liquid -> soak -> evacuate the flow cell -> clean the flow cell with sheath liquid -> evacuate the flow cell -> fill the flow cell with sheath liquid.

[0044] When "evacuating the flow cell", the normally closed ends of the three-way solenoid valve SV7 and the three-way solenoid valve SV9 are put into the conducting state, and the normally open ends of the three-way solenoid valve SV3 and the three-way solenoid valve SV8 are put into the conducting state. The rear end of the flow cell assembly 10 is connected to the atmosphere. By rotating the peristaltic pump P1 in the reverse direction, the liquid inside the moving chamber is evacuated, and the evacuated liquid flows through the three-way solenoid valve SV9 into the cleaning tank and is discharged with the waste liquid. A one-way valve is connected to the normally closed end of the three-way solenoid valve SV7 to prevent liquid from flowing out from this end;

[0045] When "cleaning the flow cell with cleaning liquid", the normally closed end of the three-way solenoid valve SV2 is put into the conducting state, so that the cleaning liquid is in a conducting connection state with the three-way solenoid valve SV2. The normally open ends of the three-way solenoid valve SV9, the three-way solenoid valve SV8, and the three-way solenoid valve SV3 are put into the conducting state. The peristaltic pump P1 is rotated to make the cleaning liquid enter the flow cell assembly 10 to achieve cleaning the flow cell with cleaning liquid. After the cleaning liquid fills the flow cell assembly 10, the rotation of the peristaltic pump P1 can be stopped to soak the flow cell assembly 10 to enhance the cleaning effect;

[0046] When "cleaning the flow cell with sheath fluid", the normally open end of the three-way solenoid valve SV2 is turned on, so that the sheath fluid is in a conducting connection state with SV2. The normally open ends of the three-way solenoid valve SV9, the three-way solenoid valve SV8, and the three-way solenoid valve SV3 are turned on. The peristaltic pump P1 is rotated to allow the sheath fluid to enter the flow cell assembly 10. After a certain period of time, the rotation of the peristaltic pump P1 is stopped. At this time, the sheath fluid is between the pipeline of SV3 and the flow cell assembly 10, and its function is to further clean, and at the same time, the cleaning fluid that may remain between the three-way solenoid valve SV9 and the peristaltic pump P1, and between the peristaltic pump P1 and the three-way solenoid valve SV8 is discharged into the cleaning fluid pipeline to ensure that no cleaning fluid flows into the corresponding pipeline when the sheath flow system 20 works;

[0047] When "filling the flow cell with sheath fluid", it is necessary to use the sheath flow system 20 to inject the sheath fluid into the flow cell assembly 10. At this time, the normally closed end of the three-way solenoid valve SV3 is opened, and the normally closed two-way solenoid valve SV10 is closed. In this way, during the filling process, the sheath flow will enter from the first connection port 103 of the flow cell assembly 10 and flow out from the second connection port 104. After the pipeline between the flow cell assembly 10 and the three-way solenoid valve SV3 is completely filled, then the normally closed two-way solenoid valve SV10 is opened and the three-way solenoid valve SV3 is closed. At this time, the sheath flow enters from the first connection port 103 of the flow cell assembly 10, and the waste liquid outlet 105 of the flow cell assembly 10 discharges. After a certain period of time, it is ensured that the inside of the flow cell assembly 10 is completely filled with the sheath fluid.

[0048] The waste discharge system 50 includes a diaphragm pump P3, a cleaning pool W1, and a normally closed two-way solenoid valve SV10. The waste liquid bucket is connected to the diaphragm pump P3, and the diaphragm pump P3 is connected to the cleaning pool W1. See Figure 6 。

[0049] The waste liquid enters the cleaning pool through the inlet 1 of the cleaning pool W1. The outlet of the cleaning pool is connected to the diaphragm pump P3. During the waste discharge process, the diaphragm pump P3 works to achieve the purpose of active waste discharge. At the same time, the top of the cleaning pool is open, and the continuous operation of the diaphragm pump P3 will not affect the liquid path system.

[0050] The above are only the preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims

1. A fluid path system for flow fluorescence, characterized in that: The invention comprises a flow chamber assembly (10), a sheath flow system (20), a sample loading system (30), a cleaning system (40) and a waste discharge system (50); the flow chamber assembly (10) comprises a flow chamber (100), a rectifying cavity (101) and an injection needle (102) which are connected in sequence, the side of the rectifying cavity (101) is respectively provided with a first connecting port (103) and a second connecting port (104), the top of the flow chamber (100) is provided with a waste liquid outlet (105), wherein the first connecting port (103) is connected to the sheath flow system (20), the second connecting port (104) is connected to the cleaning system (40), the injection needle (102) is connected to the sample loading system (30), and the waste liquid outlet (105) is connected to the waste discharge system (50).

2. A flow fluorescence liquid path system according to claim 1, characterized in that: The sheath flow system (20) includes a three-way solenoid valve SV2, a three-way solenoid valve SV9, a peristaltic pump P1, a three-way solenoid valve SV8, a normally closed two-way solenoid valve SV5, a filter F1 and a sheath liquid pressure sensor PS1. The three-way solenoid valve SV2 is connected to the sheath liquid barrel and the three-way solenoid valve SV9 respectively, the three-way solenoid valve SV9 is connected to the three-way solenoid valve SV8, a peristaltic pump P1 is connected between the three-way solenoid valve SV9 and the three-way solenoid valve SV8, the three-way solenoid valve SV8 is connected to the liquid inlet of the filter F1, the liquid outlet of the filter F1 is connected to the normally closed two-way solenoid valve SV5, the exhaust port of the filter F1 is connected to the normally closed two-way solenoid valve SV1, the normally closed two-way solenoid valve SV5 is connected to the first connecting port (103), and the sheath liquid pressure sensor PS1 is connected to the second connecting port (104).

3. A flow fluorescence liquid path system according to claim 1, characterized in that: The sample loading system (30) uses a plunger pump P2 to quantitatively absorb and push out samples. The sample loading system (30) includes a sampling needle (300), a first sample loading pipeline (301), a three-way clamp solenoid valve SV6, a second sample loading pipeline (302), a plunger pump P2 and a third sample loading pipeline (303). The plunger pump P2 is connected to the three-way solenoid valve SV4. The solenoid valve SV4 is connected to the common end of the three-way clamp solenoid valve SV6 through the second sample loading pipeline (302). The normally closed end of the three-way clamp solenoid valve SV6 is connected to the injection needle (102) of the flow chamber (100) assembly through the third sample loading pipeline (303). The normally open end of the three-way clamp solenoid valve SV6 is connected to the sampling needle (300) through the first sample loading pipeline (301).

4. A flow fluorescence liquid path system according to claim 3, characterized in that: The inner diameter of the first sample loading pipeline (301) is 0.5 mm-0.8 mm, the inner diameter of the second sample loading pipeline (302) is 0.5 mm-1.0 mm, and the inner diameter of the third sample loading pipeline (303) is approximately 0.3 mm-0.8 mm.

5. The flow fluorescence liquid path system according to claim 1, characterized in that: The cleaning system (40) includes a three-way solenoid valve SV2, a three-way solenoid valve SV9, a three-way solenoid valve SV8, and a peristaltic pump P1 shared with the sheath flow system (20), and also includes a three-way solenoid valve SV3, a cleaning pool W1 and a three-way solenoid valve SV7. The cleaning liquid barrel is connected to the three-way solenoid valve SV2, the three-way solenoid valve SV2 is connected to the three-way solenoid valve SV9, the three-way solenoid valve SV9 is connected to the three-way solenoid valve SV8, and a peristaltic pump P1 is arranged between the three-way solenoid valve SV9 and the three-way solenoid valve SV8. The solenoid valve SV8 is connected to the three-way solenoid valve SV3, and the three-way solenoid valve SV3 is respectively connected to the second connection port (104) of the flow chamber component (10) and the cleaning pool W1, and the common end of the three-way solenoid valve SV7 is connected to the waste liquid outlet (105) of the flow chamber component (10).

6. A flow fluorescence liquid path system according to claim 1, characterized in that: The waste discharge system (50) comprises a diaphragm pump P3, a cleaning tank W1 and a normally closed two-way solenoid valve SV10; the waste liquid tank is connected to the diaphragm pump P3, and the diaphragm pump P3 is connected to the cleaning tank W1.

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