Microplate reader device capable of automatically taking and placing elisa plate

Abstract

A microplate reader device capable of automatically taking and placing an elisa plate comprises an electric linear sliding table, a microplate reader body, an electric push rod, a finger cylinder, a PLC (programmable logic controller), a motor speed reducing mechanism, a detection rack and an air compressor, a shaft hole is formed in one side of a sample placing opening of the microplate reader body, one side of a bin door is rotatably mounted in the shaft hole, and the motor speed reducing mechanism is mounted in a mounting groove; the rotating shaft side end of the motor reducing mechanism and the other side of the bin door are mounted together; the detection rack comprises a supporting plate and a sample bin, a first electric linear sliding table is mounted at the rear lower part in a shell of the microplate reader body, and a second electric linear sliding table is mounted at the upper part of a sliding block of the first electric linear sliding table; the rear side end of the electric push rod is installed at the front side end of the sliding block of the second electric linear sliding table, and the rear side end of the finger air cylinder is installed at the front side end of the electric push rod. According to the utility model, a plurality of samples can be detected one by one, and the samples are placed in situ after detection, so that convenience is brought to workers, and the detection efficiency is correspondingly improved.

Description

Technical Field

[0001] The utility model relates to the technical field of detection equipment, in particular to an enzyme labeling instrument device which can automatically take and place an enzyme labeling plate. Background Art

[0002] The microplate reader is a very important testing equipment with great application value. It is widely used in major hospitals, university research institutions, disease control centers, technical quality supervision bureaus, animal and plant inspection and quarantine, food and feed industry and many other industries. In the application of the microplate reader, the inspector opens the door of the sample opening at the front of the shell, and then puts the sample to be tested (located in the plastic microplate) on the tray inside the test chamber. After closing the door, the inspector operates the test button at the front of the shell (only one press is needed to test the sample) to test the sample. The core component of the existing microplate reader, the photoelectric detector, is a single detector, either based on a CCD detector (photodiode array) or a photomultiplier tube detector. During the test, the light wave emitted by the light source lamp at the upper end of the microplate reader is converted into a monochromatic light through a filter or a monochromator, and enters the sample to be tested in the plastic microplate. Then part of the monochromatic light is absorbed by the sample, and the other part passes through the sample through a transparent tray to irradiate the photoelectric detector at the lower end of the microplate reader. The photoelectric detector inputs the different light signals of the sample to be tested and the different strengths into the signal input end of the microplate reader conversion board, and the conversion board converts it into a corresponding electrical signal. After pre-amplification, logarithmic amplification, analog-to-digital conversion and other signal processing, the electrical signal is sent to the microprocessor for data processing and calculation. Finally, the data results of the test sample (such as allergens, thyroid, inorganic toxins, etc.) are displayed on the display screen, or the data results are printed out through an external printer.

[0003] Although the existing ELISA instruments meet the detection needs to a certain extent, they are limited by the structure. Each time a sample is detected, the door is opened manually to put the sample in the plastic microplate to be detected into the device for detection. After the detection is completed, the door is opened, the sample is taken out and closed. When a large number of samples need to be detected, it will cause inconvenience to the detection personnel, which is not conducive to improving the detection efficiency. With the advancement of industrial technology, the technology based on PLC or single-chip microcomputer and host computer to control the operation of related electrical equipment (such as motor reduction mechanism, electric lead screw slide, solenoid valve, etc.) according to the program has been widely used in industrial production, etc., but it has not been applied in ELISA instrument technology. In summary, it is particularly necessary to provide an ELISA instrument device that does not require manual operation and can automatically detect a certain number of different samples. Utility Model Content

[0004] In order to overcome the drawbacks of existing ELISA instruments due to structural limitations as described in the background technology, the utility model provides an ELISA instrument device that is based on the ELISA instrument body. During application, relevant mechanisms work together to automatically open the door and place the samples to be tested one by one into the testing station for testing, and put the samples back to their original positions after testing, thereby bringing convenience to the staff and correspondingly improving the testing efficiency. The device can automatically take and place ELISA plates.

[0005] The technical solution adopted by the utility model to solve its technical problems is:

[0006] An ELISA device capable of automatically taking and placing ELISA plates comprises an electric linear slide, an ELISA body, an electric push rod, a finger cylinder, a PLC, a motor reduction mechanism, a test rack, and an air compressor. The electric linear slide has two sets, the multi-channel power output end of the PLC is electrically connected to the two sets of electric linear slides, the electric push rod, the motor reduction mechanism, and the electromagnetic valves on both sides of the finger cylinder, the exhaust pipe of the air compressor and the air inlet pipes of the electromagnetic valves on both sides of the finger cylinder are connected via hoses, and the device is characterized in that a shaft hole is provided on one side of the sample opening of the shell of the ELISA body, a mounting groove is provided on the other side of the sample opening, and a compartment door on one side of the ELISA body is rotatably installed. In the shaft hole, the motor reduction mechanism is installed in the installation groove, and the side end of the rotating shaft of the motor reduction mechanism is installed together with the other side of the bin door; the detection material rack includes a support plate and a sample bin, and multiple sample bins are installed together from top to bottom, and the lower end sample bin is installed at the upper end of the support plate, each sample bin has multiple sample placement boxes, the first set of electric linear slides is installed in the rear lower part of the outer shell of the ELISA instrument body, and the second set of electric linear slides is installed on the upper part of the sliding block of the first set of electric linear slides; the rear side end of the electric push rod is installed on the front side end of the sliding block of the second set of electric linear slides, and the rear side end of the finger cylinder is installed on the front side end of the electric push rod.

[0007] Furthermore, the height and width of the sample placement opening are respectively greater than the height and width of the detection rack.

[0008] Furthermore, when the push column of the electric push rod is located at the rear dead point, the finger of the finger cylinder is located at the rear side end of the tray inside the detection chamber of the ELISA instrument body.

[0009] Furthermore, the detection material rack is placed at the front side end of the detection chamber of the ELISA instrument body.

[0010] Furthermore, the front ends of the plurality of sample placement boxes are open structures, and each sample placement box is loaded with a test sample.

[0011] Furthermore, one of the power output ends of the PLC is electrically connected to both ends of the power input of a relay, and the relay control contact end and the normally open contact end are electrically connected to two contacts under the detection button of the ELISA instrument body.

[0012] The beneficial effects of the utility model are as follows: the utility model is based on the body of the microplate reader, combined with the existing mature PLC control technology of related electrical equipment, and can automatically control the opening and closing of the compartment door through the motor reduction mechanism. Through the coordinated action of two sets of electric linear slides, electric push rods, finger cylinders, etc., the plastic microplates loaded with test samples placed in each sample placement box of the test material rack can be placed one by one from top to bottom, and the test station in the body of the microplate reader can be tested. After the test, the samples are put back in place, which brings convenience to the staff and correspondingly improves the test efficiency. Based on the above, the utility model has a good application prospect. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] The utility model is further described below in conjunction with the accompanying drawings and embodiments.

[0014] Figure 1 It is a schematic diagram of the overall structure and the partially enlarged structure of the utility model.

[0015] Figure 2 , 3 4 is a schematic diagram of the local structure of the utility model.

[0016] Figure 5 This is a circuit diagram of the utility model. DETAILED DESCRIPTION

[0017] Figure 1 , 2As shown in , 3, 4, and 5, an ELISA device capable of automatically taking and placing ELISA plates includes small electric linear slides M1 and M2, an ELISA body 1, a small electric push rod M3, a small finger cylinder 3, a PLC W2, a small motor reduction mechanism M4, a detection rack 4, a power module W1, and a small air compressor (not shown in the figure, 300W). There are two sets of electric linear slides M1 and M2, a multi-channel power output end of the PLC W2 and two sets of electric linear slides M1 and M2 (3, 4 feet and 5, 6 feet of the PLC), an electric push rod M3 (PLC The power input ends of the electromagnetic valves DC1 and DC2 (pins 11 and 12 and pins 13 and 14 of the PLC) at both sides of the finger cylinder 3 are connected via wires, respectively. The exhaust pipe of the air compressor's air storage tank and the air inlet pipes of the electromagnetic valves at both sides of the finger cylinder 3 are connected in parallel via hoses, and the hose with a length margin connected to the finger cylinder 3 is led out through the opening at the rear side end of the detection chamber of the microplate reader body 1 (the opening is sealed with a sealant); the lower left end of the sample opening 101 of the shell of the microplate reader body There is an axial hole, and the right end of the sample opening 101 has a recessed mounting groove 102. The lower left end of the door 103 of the ELISA instrument body is rotatably mounted in the axial hole. The motor reduction mechanism M4 is horizontally mounted in the mounting groove 102. The left end of the rotating shaft of the motor reduction mechanism M2 and the lower right end of the door 103 are welded together; the detection rack includes a support plate 41 and a sample bin 42. The four sample bins 42 are welded together from top to bottom, and the lower end sample bin 42 is welded to the upper end of the support plate 41. Four partitions are distributed at equal distances in each sample bin to divide the sample bin into five An independent sample placement box 43; the first set of electric linear slides M1 is horizontally installed at the lower rear end of the detection chamber of the ELISA instrument body 1, and the second set of electric linear slides M2 is vertically distributed, and the lower end of its shell is vertically installed on the upper part of the sliding block of the first set of electric linear slides M1; the rear end of the cylinder of the electric push rod M3 is longitudinally and horizontally installed on the front end of the sliding block of the second set of electric linear slides M2, and the middle part of the rear end of the finger cylinder 3 is horizontally and longitudinally installed on the front end of the push column of the electric push rod M3; the power module W1 is installed in the component chamber of the ELISA instrument body 1.

[0018] Figure 1 , 2As shown in , 3, 4, and 5, the height and width of the sample opening 101 are respectively greater than the height and width of the detection rack 4. When the push column of the electric push rod M3 is at the rear dead point, the finger of the finger cylinder 3 is located at the rear end of the tray inside the detection chamber of the ELISA instrument body 1. The detection rack 4 is placed at the front end of the chamber door 103 of the ELISA instrument body. The front ends of the twenty sample placement boxes 43 are open structures, and each sample placement box 43 is placed with a plastic microplate 2 loaded with the detection sample. One of the power output terminals 15 and 16 of the PLC and the two ends of the power input of a relay K1 are connected by wires, and the control contact end and the normally open contact end of the relay K1 are connected to the two contacts under the detection button S1 of the ELISA instrument body by wires. The power input terminals 1 and 2 of the power module W1 are connected to the two poles of the AC 220V power supply by wires; the power output terminals 3 and 4 of the power module W1 and the power input terminals 1 and 2 of the PLC are connected by wires.

[0019] Figure 1 , 2 As shown in , 3, 4, and 5, the present invention is based on an ELISA body 1. Before the test, the door 103 on the front side of the sample opening of the ELISA body 1 is opened, and a sample 2 to be tested (located in a plastic microplate) is placed on a tray inside the test chamber. After the door 103 is closed, the sample can be tested by pressing the test button S1 of the ELISA body 1. During the test, the light wave emitted by the light source lamp at the upper end of the ELISA body 1 is converted into a monochromatic light through a filter or a monochromator, and enters the sample 2 to be tested in the plastic microplate. Then, part of the monochromatic light is absorbed by the sample, and the other part is absorbed by the sample. The specimen is irradiated through the transparent tray onto the photoelectric detector at the lower end of the microplate reader body 1. The photoelectric detector inputs the light signal of different strengths of the specimen to be tested into the signal input end of the conversion board of the microplate reader body 1. The conversion board converts it into a corresponding electrical signal. The electrical signal is sent to the microprocessor for data processing and calculation after pre-amplification, logarithmic amplification, analog-to-digital conversion and other signal processing. Finally, the data results of the test sample (such as allergens, thyroid, inorganic toxins, etc.) are displayed on the display screen, or the data results are printed out through a peripheral supporting printer. The above is an existing mature technology, and this application does not provide any protection.

[0020] Figure 1 , 2As shown in , 3, 4, and 5, after the 220V AC power enters the power input terminal of the power module W1, the 3rd and 4th pins of the power module W1 output a DC 24V power supply and enter the power input terminal of the PLC. In this application, the specific detection process is as follows. (1): The 9th and 10th pins of the PLC output positive and negative power to the two-pole power input terminal of the motor reduction mechanism M4 for a period of time, and the shaft of the motor reduction mechanism M4 controls the warehouse door 103 to rotate counterclockwise about 90 degrees and stop, so that the warehouse door opens. (2): The 3rd and 4th pins of the PLC output positive and negative power or negative and positive power to the power input terminal of the electric linear slide M1 for a period of time, so that the electric linear slide M1 will move from left to right or from right to left, and the detection sample 2 in a plastic microplate on the detection rack 4 is clamped by the finger cylinder 3 and removed from the detection rack 4 and placed on the tray inside the detection bin for detection (after detection, the PLC reverses the control to place the detection sample 2 into the original corresponding sample placement box 43); the 5th and 6th pins of the PLC output Output positive and negative power or negative and positive power to the power input terminal of the electric linear slide M2 ​​for a period of time, so that the electric linear slide M2 ​​will move from top to bottom or from bottom to top, and take the test sample 2 in a plastic microplate on the test rack 4, clamp it from the test rack 4 through the finger cylinder 3 and put it on the tray inside the test bin for testing (after testing, PLC reverses control to put the test sample 2 into the original corresponding sample placement box 43); the 7th and 8th pins of the PLC output positive and negative power or negative and positive power for a period of time. The bipolar power supply is connected to the power input end of the electric push rod M3, so that the push column of the electric push rod M3 will move from back to front or from front to back, and the test sample 2 in a plastic microplate on the test rack 4 is clamped by the finger cylinder 3 and taken out from the test rack 4 and placed on the tray inside the test chamber for testing (after testing, the PLC reverses the control to put the test sample 2 into the original corresponding sample placement box 43); in this process of the present application, the PLC controls the electric linear slides M1, M2 and the electric push rods M3, the finger cylinder 3, etc. , a test sample 2 in a plastic microplate on the test rack 4 is clamped by the finger cylinder 3 and taken out from the test rack 4 and placed on the tray inside the test chamber for testing. Four test samples 2 are taken out from the sample placement box 43 on the left side of the test rack 4 from top to bottom for testing, and then four test samples 2 are taken out from the sample placement box 43 in the middle of the test rack 4 from top to bottom for testing, and finally four test samples 2 are taken out from the sample placement box 43 on the right side of the test rack 4 from top to bottom for testing.(3): When the electric linear slides M1, M2 and the electric push rod M3 drive the finger cylinder 3 to the inside of one of the sample placement boxes 43 of the test rack 4 (two fingers are located at the two sides of a test sample 2) or the test sample 2 needs to be clamped on the tray (two fingers are located at the two sides of a test sample 2), the PLC (pins 11 and 12 output power for a period of time) controls the solenoid valve DC1 on the left side of the clamping cylinder to be energized and work for a period of time, and its valve core opens. In this way, the compressed air in the air compressor tank enters the air inlet duct at the left end of the finger cylinder 3, and the left end finger of the finger cylinder 3 moves toward the right end to clamp the test sample 2 (the air in the air inlet duct at the right end of the finger cylinder 3 is discharged through the exhaust port of the solenoid valve DC2); when the electric linear slides M1, M2 and the electric push rod M3 When the finger cylinder 3 is driven to the inner side of a corresponding sample placement box 43 of the detection rack 4 or the tray where the detection sample 2 needs to be put down (two fingers are respectively located at the two side ends of a detection sample 2), the PLC (13 and 14 feet output power for a period of time) controls the solenoid valve DC2 at the right end of the clamping cylinder to be energized and work for a period of time, and its valve core opens. In this way, the compressed air of the air compressor tank enters the air inlet duct at the right end of the finger cylinder 3, and the left end finger of the finger cylinder 3 moves toward the left end to release the detection sample 2 (the air in the air inlet duct at the left end of the finger cylinder 3 is discharged through the exhaust port of the solenoid valve DC1, and the air is discharged to the outside from the opening at the upper rear end of the shell), and the detected detection sample 2 can be placed in the inner side of a corresponding sample placement box 43, or the detection sample 2 to be detected is placed on the tray for detection. (4): Pins 9 and 10 of the PLC output a positive and negative power supply to the power input terminal of the motor reduction mechanism M4 for a period of time. The shaft of the motor reduction mechanism M4 controls the compartment door 103 to rotate clockwise about 90 degrees and stop. In this way, the compartment door 103 will be closed and enter the detection process. (6) Pins 15 and 16 of the PLC output a positive and negative power supply to the power input terminal of the relay K1 for a period of time. The relay K1 will be energized and attracted for a period of time. Its control contact end and normally open contact end are closed. In this way, during the time when the relay K1 is energized, the two contacts under the detection button S1 of the microplate reader body 1 will be closed, and the microplate reader body 1 can automatically perform the sample detection process. The above steps are continuously cycled, and the new type can place the plastic microplate containing the detection sample placed in the box of each sample rack from top to bottom, and place it in the detection station in the microplate reader body for detection. After the detection, the sample is placed in place, which brings convenience to the staff and correspondingly improves the detection efficiency.

[0021] Figure 1 , 2As shown in , 3, 4, and 5, the power module W1 is a finished product of a switch module from AC 220V to DC 24V; the relay K1 model is 4123 / DC24V; the electric push rod M3 is a finished product of a small reciprocating electric telescopic rod (power 10W); the electric linear slides M1 and M2 are finished products of electric lead screw slides with a power of 20W; the motor reduction mechanism M4 is a finished product of a coaxial motor gear reducer (power 10W); and the PLC model is S7-200SMART. It should be noted that the PLC controls the interval time (including cyclic output) of its multiple power output terminals to output power to the power-consuming equipment, which is an extremely mature technology. This application does not provide any protection for the PLC to control the interval time (including cyclic output) of its multiple power output terminals to output power to the power-consuming equipment. This application protects the technical solution that the PLC can control the relevant components to automatically detect multiple samples in a cycle.

[0022] The above shows and describes the basic principles and main features of the utility model and the advantages of the utility model. For those skilled in the art, it is obvious that the utility model is limited to the details of the above exemplary embodiments, and the utility model can be implemented in other specific forms without departing from the spirit or basic features of the utility model. Therefore, no matter from which point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the utility model is defined by the attached claims rather than the above description, and it is intended to include all changes within the meaning and scope of the equivalent elements of the claims. Any figure mark in the claims should not be regarded as limiting the claims involved.

[0023] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. An ELISA device capable of automatically taking and placing ELISA plates, comprising an electric linear slide, an ELISA body, an electric push rod, a finger cylinder, a PLC, a motor reduction mechanism, a test rack, and an air compressor. The electric linear slide has two sets, the multi-channel power output end of the PLC and the two sets of electric linear slides, the electric push rod, the motor reduction mechanism and the electromagnetic valves on both sides of the finger cylinder are electrically connected, and the exhaust pipe of the air compressor and the air inlet pipe of the electromagnetic valve on both sides of the finger cylinder are connected via hoses, respectively. The invention is characterized in that: The shell of the enzyme marker body has an axial hole on one side of the sample opening, and a mounting groove on the other side of the sample opening. One side of the chamber door of the enzyme marker body is rotatably mounted in the axial hole, and the motor reduction mechanism is mounted in the mounting groove. The side end of the rotating shaft of the motor reduction mechanism and the other side of the chamber door are mounted together; the detection material rack includes a support plate and a sample chamber, and a plurality of sample chambers are mounted together from top to bottom, and the lower end sample chamber is mounted on the upper end of the support plate, and each sample chamber has a plurality of sample placement boxes. The first set of electric linear slides is mounted on the lower rear part of the shell of the enzyme marker body, and the second set of electric linear slides is mounted on the upper part of the sliding block of the first set of electric linear slides; The rear side end of the electric push rod is installed on the front side end of the sliding block of the second set of electric linear slides, and the rear side end of the finger cylinder is installed on the front side end of the electric push rod.

2. The ELISA instrument capable of automatically taking and placing ELISA plates according to claim 1, characterized in that: The height and width of the layout opening are respectively greater than the height and width of the detection material rack.

3. The ELISA instrument capable of automatically taking and placing ELISA plates according to claim 1, characterized in that: When the push column of the electric push rod is located at the rear dead point, the finger of the finger cylinder is located at the rear side end of the tray inside the detection chamber of the enzyme labeling instrument body.

4. The ELISA instrument capable of automatically taking and placing ELISA plates according to claim 1, characterized in that: The test material rack is placed at the front side of the test chamber of the ELISA instrument body.

5. The ELISA instrument capable of automatically taking and placing ELISA plates according to claim 1, characterized in that: The front ends of the multiple sample placement boxes are open structures, and each sample placement box is loaded with a test sample.

6. The ELISA instrument capable of automatically taking and placing ELISA plates according to claim 1, characterized in that: One of the power output terminals of the PLC is electrically connected to both ends of the power input of a relay, and the control contact terminal and the normally open contact terminal of the relay are electrically connected to two contacts under the detection button of the ELISA instrument body.

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