Gel supernatant filter
By designing a gel supernatant filter, the filter mesh and frozen storage tube are used to separate the supernatant from the gel, solving the problems of complex separation process and easy contamination in the prior art, and improving the separation efficiency and sterility.
Patent Information
- Application Number
- CN202421611738.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-09
- Publication Date
- 2025-05-30
- Estimated Expiration
- 2034-07-09
AI Technical Summary
In the prior art, the process of making PRP gel supernatant is complicated, the operation is difficult to absorb the supernatant, there is a risk of misoperation, and it is easy to cause secondary contamination, affecting the quality of the PRP gel and supernatant.
A gel supernatant filter is designed, including preparation tubes, frozen storage tubes, filter mesh and injection tubes. By injecting PRP and calcium chloride solutions, the gel is left to stand, and the supernatant is collected inverted. The filter blocks the gel to achieve separation of the supernatant and gel.
The separation process of the supernatant is simplified, the operation difficulty and secondary contamination risk are reduced, the sterile state is ensured, the separation time is shortened, and the separation efficiency is improved.
Smart Images
Figure CN222918265U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of experimental filtration devices, particularly to a gel supernatant filter. Background Art
[0002] PRP (platelet-rich plasma) gel is a biomaterial widely used in the fields of medicine and beauty. It shows significant efficacy in aspects such as wound repair, skin regeneration, and anti-aging. During the production of PRP gel, there is a small amount of liquid residue, and this supernatant is rich in a large number of cell growth factors. Research shows that separating and using the supernatant separately from the gel can better exert its therapeutic effect. Therefore, how to effectively separate and utilize this part of the supernatant has become an important topic.
[0003] Currently, the method for producing PRP gel supernatant is relatively complex and requires strict adherence to multiple operating steps. First, extract PRP from a blood sample and place it in a clean centrifuge tube for standby. Prepare a calcium chloride solution and also place it in a centrifuge tube for standby. Next, use a syringe to draw the PRP solution and the calcium chloride solution respectively, and slowly add the two into a container and gently mix them evenly. Let the activated PRP stand at room temperature for a period of time. At this time, the calcium ions provided by calcium chloride activate the platelets in the PRP, prompting them to release growth factors and promoting the conversion of fibrinogen into fibrin, thereby forming a network structure that captures platelets and cells, and finally converting the PRP solution into a gel-like substance while producing a small amount of liquid supernatant. To obtain the supernatant, a syringe or pipette is needed to suck out the supernatant from the gel.
[0004] However, the overall process is rather cumbersome, the operation of sucking out the supernatant is difficult, there is a risk of misoperation, and secondary contamination is likely to occur during the operation, which may affect the quality of both the PRP gel and the supernatant. Utility Model Content
[0005] In order to reduce the difficulty of separating the supernatant from the gel, this application provides a gel supernatant filter.
[0006] The gel supernatant filter provided by this application adopts the following technical solution:
[0007] A gel supernatant filter includes a preparation tube with an open upper end and a cryopreservation tube that covers the opening of the preparation tube from the upper end. The preparation tube is in communication with the cryopreservation tube. The upper end of the preparation tube is detachably connected with a filter screen that shields the opening of the preparation tube. The side wall of the preparation tube is in communication with an injection tube below the filter screen, and the opening of the injection tube is detachably connected with a tube plug.
[0008] By adopting the above technical solution, the cryopreservation tube is used to cover and prepare the tube. At this time, the upper end of the preparation tube is not connected to the outside. Subsequently, PRP and calcium chloride solution are injected into the preparation tube through the nozzle of the injection tube respectively. After standing for a period of time until a gel-like substance is produced, the nozzle of the injection tube is then sealed, and the preparation tube is inverted so that the gel supernatant is collected into the cryopreservation tube, and the gel is blocked by the filter screen. Next, only the cryopreservation tube needs to be disassembled to achieve the separation of the supernatant and the gel. Compared with the existing steps, the solution of the present application can not only simplify the steps, but also prevent secondary contamination during operation, ensure the aseptic state, thereby shortening the separation time and improving the separation efficiency.
[0009] Optionally, the nozzle plug is provided with a syringe socket.
[0010] By adopting the above technical solution, during injection, the syringe can be inserted into the syringe socket to fix the orientation of the syringe, thereby avoiding the decrease in the final yield caused by the injection liquid hanging on the wall of the preparation tube.
[0011] Optionally, the upper end of the preparation tube is threadedly connected to the nozzle of the cryopreservation tube.
[0012] Optionally, the upper end of the preparation tube is provided with an internal thread interface, and the nozzle of the cryopreservation tube is provided with an external thread interface that matches the internal thread interface.
[0013] By adopting the above technical solution, the cryopreservation tube is inserted into the opening of the preparation tube, which helps to avoid the overflow of liquid from the threaded connection when inverted.
[0014] Optionally, the nozzle of the cryopreservation tube is detachably connected with a tube cap.
[0015] By adopting the above technical solution, after the supernatant is separated, the nozzle of the cryopreservation tube is covered with a tube cap, which is convenient for later independent preservation of the supernatant and batch processing.
[0016] Optionally, the preparation tube includes a columnar part and a reaction part arranged in sequence from top to bottom. The columnar part is communicated with the injection tube, and the inner diameter of the reaction part gradually decreases from top to bottom.
[0017] By adopting the above technical solution, the gradually decreasing inner diameter makes the peripheral surface of the reaction part form an inward inclined surface, which helps to guide the liquid added into the preparation tube to react.
[0018] In summary, the beneficial technical effects of the present application are as follows: The present application combines a preparation device and a cryopreservation device, and uses a filter screen arranged at the upper end of the preparation tube to separate the supernatant and the gel. The operation is simple and easy, which can effectively ensure the aseptic state during the reaction and liquid extraction, and is not easy to cause secondary contamination during operation. Generally speaking, it shortens the separation time and improves the separation efficiency. Description of the Drawings
[0019] Figure 1 It is a cross-sectional schematic view of the gel supernatant filter in the embodiment of the present application.
[0020] Explanation of reference numerals: 1, preparation tube; 11, columnar part; 12, reaction part; 13, injection tube; 2, cryopreservation tube; 3, tube mouth plug; 31, syringe socket; 4, filter screen. Detailed implementation manners
[0021] The following will further describe the present application in detail Figure 1 with reference to the accompanying drawings.
[0022] The embodiment of the present application discloses a gel supernatant filter. Referring to Figure 1 , the gel supernatant filter includes a preparation tube 1 with an open upper end and a cryopreservation tube 2 that covers the tube mouth of the preparation tube 1 from the upper end, and the preparation tube 1 is communicated with the cryopreservation tube 2. The preparation tube 1 includes a columnar part 11 and a reaction part 12 that are integrally formed in sequence from top to bottom, and the inner diameter of the reaction part 12 gradually decreases from top to bottom.
[0023] An injection tube 13 for injecting PRP and calcium chloride solution is communicated with the side wall of the columnar part 11, and a tube mouth plug 3 is detachably connected to the tube mouth of the injection tube 13. The tube mouth plug 3 can be a conventional silicone plug or glass plug. When it is necessary to add liquid into the preparation tube 1, the tube mouth plug 3 can be pulled out for injection, and the injected liquid does not hang on the wall during injection; the tube mouth plug 3 can also be provided with a syringe socket 31 through it. During injection, the syringe is inserted into the syringe socket 31 to fix the orientation. The present application shows an example with the latter.
[0024] A filter screen 4 that shields the tube mouth of the preparation tube 1 is detachably connected to the upper end of the columnar part 11, and the filter screen 4 is arranged above the injection tube 13. In a specific implementation manner, the filter screen 4 is embedded in the upper end of the columnar part 11, and the outer peripheral surface of the filter screen 4 abuts against the tube wall of the columnar part 11; in another specific implementation manner, the middle part of the outer periphery of the filter screen 4 is recessed and an O-ring is embedded. When the filter screen 4 is installed on the upper end of the columnar part 11, the O-ring abuts against the tube wall of the columnar part 11. The present application shows an example with the first specific implementation manner.
[0025] An internal thread interface is processed and formed at the upper end of the columnar part 11, and an external thread interface that matches the internal thread interface is processed and formed at the tube mouth of the cryopreservation tube 2, so that the upper end of the columnar part 11 is threadedly connected to the tube mouth of the cryopreservation tube 2. After the supernatant has been separated and the cryopreservation tube 2 is detached from the preparation tube 1, a tube cap can be threadedly installed on the cryopreservation tube 2, which is convenient for realizing the independent preservation of the supernatant later and is not shown in the drawings; when the preparation tube 1 is not needed, the tube mouth of the columnar part 11 can be sealed with a silicone plug or a glass plug.
[0026] The implementation principle of the gel supernatant filter in the embodiment of this application is as follows: Keep the freezing tube 2 covering the preparation tube 1. At this time, the upper end of the preparation tube 1 is not connected to the outside. Subsequently, inject PRP and calcium chloride solution into the preparation tube 1 through the nozzle of the injection tube 13 respectively, and let it stand for a period of time until a gel-like substance is produced. Then, seal the nozzle of the injection tube 13 with a silica gel plug or a glass plug, invert the preparation tube 1, so that the gel supernatant is collected into the freezing tube 2, and the gel is blocked by the filter screen 4. Next, just disassemble the freezing tube 2 and install the tube cap 5 to achieve the separation of the supernatant; then remove the filter screen 4 to take out the PRP gel.
[0027] The above are all the preferred embodiments of this application, and the protection scope of this application is not limited thereby. Therefore, all equivalent changes made according to the structure, shape, and principle of this application shall be covered within the protection scope of this application.
Claims
1. A gel supernatant filter, characterized in that: The invention comprises a preparation tube (1) with an opening at the upper end and a cryopreservation tube (2) covering the tube mouth of the preparation tube (1) from the upper end, wherein the preparation tube (1) is connected to the cryopreservation tube (2), the upper end of the preparation tube (1) is detachably connected to a filter screen (4) for shielding the tube mouth of the preparation tube (1), the side wall of the preparation tube (1) is connected to an injection tube (13) below the filter screen (4), and the tube mouth of the injection tube (13) is detachably connected to a tube mouth plug (3).
2. The gel supernatant filter according to claim 1, characterized in that: The tube mouth plug (3) is provided with a syringe socket (31).
3. The gel supernatant filter according to claim 1, characterized in that: The upper end of the preparation tube (1) is threadedly connected to the tube mouth of the freezing tube (2).
4. The gel supernatant filter according to claim 3, characterized in that: The upper end of the preparation tube (1) is provided with an internal thread interface, and the tube mouth of the freezing tube (2) is provided with an external thread interface that matches the internal thread interface.
5. The gel supernatant filter according to claim 4, characterized in that: The tube mouth of the freezing tube (2) is detachably connected with a tube cover.
6. The gel supernatant filter according to claim 1, characterized in that: The preparation tube (1) comprises a columnar portion (11) and a reaction portion (12) arranged in sequence from top to bottom, the columnar portion (11) is connected to the injection tube (13), and the inner diameter of the reaction portion (12) gradually decreases from top to bottom.