Efficient magnetic separation elution automation equipment for IVD coating

Abstract

The utility model discloses high-efficiency magnetic separation elution automation equipment for IVD coating, which comprises a bin body and a bin door hinged with the bin body, the bin body is provided with two groups of reactors, the bin body comprises an upper space and a lower space, and the upper space and the lower space are separated by an operation table to form independent operation spaces. A mechanical arm is arranged in the upper space of the bin body and arranged on an operation table, an ultrasonic degasser is further arranged in the bin body and arranged on one side of the mechanical arm, and a weighing liquid adding position and a suction pipe support position are arranged between the two reactors arranged on the side away from the ultrasonic degasser. A liquid suction pump and a liquid adding pump are arranged in the lower space of the bin body, a solvent in the reactor is pumped through the liquid suction pump and discharged into a waste liquid barrel arranged in the lower space of the bin body, and a solvent in a reagent barrel arranged in the lower space of the bin body is added into a solution bottle through the liquid adding pump.

Description

Technical Field

[0001] The utility model belongs to the technical field of IVD in-vitro diagnostic magnetic bead coating production, and particularly relates to an efficient magnetic separation dewashing automation device for IVD coating. Background Technique

[0002] In-vitro diagnosis (IVD) refers to taking samples such as blood, body fluids, and tissues out of the human body and using in-vitro detection reagents, test kits, etc. to detect the samples.

[0003] With the maturity of magnetic bead IVD technology, this method has become the most mainstream technical method. The production demand has gradually increased. Bio-magnetic beads need to be coated with antibodies of target extracts, so as to extract the target substances in later blood, body fluids, and tissues. The separation device is an essential device for magnetic bead antibody coating. In the prior art, manual production is usually used for operation. However, the single operation time of manual pipetting is long, and the work efficiency is low, which can no longer meet the batch demand and consumes manpower and material resources.

[0004] In view of the above factors, an efficient magnetic separation dewashing automation device for IVD coating is specially designed. It is fast. Compared with the traditional manual pipetting operation of personnel, the operation time is greatly shortened. One bottle can be completed in 20 minutes, and 4 bottles can be completed in 80 minutes, while manual operation requires 8 hours, improving the work efficiency. Summary of the Utility Model

[0005] The purpose of the utility model is to provide an efficient magnetic separation dewashing automation device for IVD coating to solve the problems put forward in the above background technique.

[0006] The purpose of the utility model is realized by the following technical solutions: An efficient magnetic separation dewashing automation device for IVD coating includes a bin body and a bin door hinged to the bin body. The bin body is provided with two groups of reactors. The bin body includes an upper space and a lower space, and the upper space and the lower space are separated by an operating platform to form independent operating spaces;

[0007] A robot is arranged in the upper space of the bin body. The robot is placed on the operating platform. An ultrasonic degasser is also arranged in the bin body. The ultrasonic degasser is placed on one side of the robot. A weighing plus liquid level and a pipette support position are respectively arranged between the two groups of reactors arranged on the side far from the ultrasonic degasser;

[0008] A liquid suction pump and a liquid addition pump are arranged in the lower space of the bin body. The solvent in the reactor is pumped into a waste liquid bucket arranged in the lower space of the bin body through the liquid suction pump, and the solvent in a reagent bucket arranged in the lower space of the bin body is added into a solution bottle through the liquid addition pump;

[0009] The warehouse body is also provided with an upper / lower material level and a straw material silo, which are placed on the operating table and close to the warehouse door.

[0010] Furthermore, the robot comprises a first manipulator and a second manipulator, the first manipulator is a clamping claw structure, the clamping claw structure is a three-claw hook-shaped claw design, and it adopts pneumatic or electric three-claws, and the second manipulator is a pipe opening tooling manipulator;

[0011] The second manipulator includes a pipe holding end and a pipe withdrawing end. The pipe holding end adopts a pneumatic or electric two-claw structure. The pipe withdrawing end cooperates with the connecting end of the pipe mouth tooling. The pipe withdrawing end controls the up and down movement of the withdrawing plate by pneumatic or electric means. The front end of the withdrawing plate is concave and cooperates with the outer diameter of the pipe mouth tooling.

[0012] Furthermore, the first manipulator is provided with a spring fixture, and the spring fixture cooperates with the clamping structure of the first manipulator to fix the bottle cap;

[0013] The spring tooling includes a positioning column, which is fixedly arranged on the first manipulator and located between the clamping claw structure of the first manipulator. A positioning nut is arranged at the end of the positioning column. A spring is sleeved on the positioning column. A star-shaped positioning plate is sleeved on the positioning column. The star-shaped positioning plate is located at the bottom of the spring.

[0014] Furthermore, the upper / lower material position is rectangular, and a plurality of circular receiving grooves for receiving solution bottles are arranged in an array on the upper / lower material position.

[0015] Furthermore, a water absorption structure is arranged adjacent to one side of the ultrasonic degasser, and the water absorption structure includes a hollow outer frame connected to the operating table, the space formed by the outer frame is filled with a water-absorbing sponge, and the water-absorbing sponge on the outer frame forms a circular receiving groove for accommodating a solution bottle.

[0016] Further, the straw support position is a rectangular groove structure extending downward, and the edge of the straw support position is connected to the straw support by bolts;

[0017] The pipette support is detachably connected to a pipe opening tooling, and the pipe opening tooling is plug-fitted with the test tube.

[0018] Furthermore, a hollow waste straw collection trough is provided adjacent to the straw support, and the waste straw collection trough is rectangular or circular and fixed on the operating table.

[0019] Furthermore, the liquid suction pump is connected to the waste liquid bucket and the pipeline opening tooling respectively through connecting pipelines.

[0020] Further, the liquid addition pump is respectively connected to the reagent barrel and the pipe orifice tooling through connecting pipes.

[0021] Further, universal wheels and positioning angles are provided at the bottom of the bin body, and a UPS uninterruptible power supply is provided inside the bin body. The UPS uninterruptible power supply is arranged in the lower space below the loading / unloading position;

[0022] The bin body also includes a robot control box, and the robot control box is connected to a control platform connected to the outside of the bin body through a bracket through a connecting wire;

[0023] The control platform adopts a PIC / single-chip microcomputer controller, and the control platform is electrically connected to an ultrasonic degasser inside the bin body;

[0024] The control platform is electrically connected to a liquid suction pump and a liquid addition pump inside the bin body;

[0025] The control platform is electrically connected to two groups of reactors inside the bin body.

[0026] Compared with the prior art, the beneficial effects of the present utility model are as follows:

[0027] The system of the present utility model can record more than 200 processes. After different solvents and magnetic bead processes are determined, they can be stored in the system and directly retrieved during work, which has higher stability compared with traditional manual processes.

[0028] The present utility model does not require employees to manually record key separation, shaking, and ultrasonic time. The machine automatically records them, without errors or misrecording problems, and there is no human reading error.

[0029] The present utility model is fast. Compared with manual pipetting operations by personnel, the operation time is greatly shortened. One bottle can be completed in 20 minutes, and 4 bottles can be completed in 80 minutes, while manual operation requires 8 hours, improving work efficiency.

[0030] The present utility model adopts a jaw design to prevent the bottle from falling off. The jaw adopts a three-jaw hook-shaped jaw design and uses pneumatic or electric three jaws.

[0031] The tube connector tooling of the present utility model adopts a non-standard design for quickly connecting test tubes in the straw bin, and does not drop tubes or leak water during the liquid suction or liquid addition process.

[0032] The present utility model is provided with a straw bracket for quick tube replacement. The bracket can fix the tube connector and can achieve the purpose of stable fixation. After the liquid suction is completed, the tube withdrawal device on the second robot quickly withdraws the liquid suction tube into the waste tube barrel, which is convenient for operation.

[0033] On the operating table of the present utility model, a balance is placed below the working position to ensure accurate addition of the solution in the bottle.

[0034] Even when the power is off, the gripper of this utility model will not open and cause the phenomenon of the bottle falling off. It is equipped with a UPS, and even when the power is off, it can still operate for 1 hour. Brief Description of the Drawings

[0035] Figure 1 is a three-dimensional schematic diagram of this utility model;

[0036] Figure 2 is a top view schematic diagram of the upper space of this utility model;

[0037] Figure 3 is a three-dimensional schematic diagram of the upper / lower space of this utility model;

[0038] Figure 4 is a schematic diagram of the structure of the first manipulator of this utility model;

[0039] Figure 5 is a schematic diagram of the structure of the second manipulator of this utility model;

[0040] Figure 6 is a schematic diagram of the cooperation between the straw support and the pipe orifice tooling of this utility model;

[0041] Figure 7 is a schematic diagram of the spring tooling of this utility model;

[0042] Figure 8 is a plan view schematic diagram of the spring tooling of this utility model. Detailed Description of the Preferred Embodiments

[0043] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present utility model.

[0044] In the description of the present utility model, it should be noted that unless otherwise clearly defined and limited, the terms "installation", "connection", and "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a direct connection, or an indirect connection through an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific situations.

[0045] In the description of the present utility model, it should be understood that the orientation or positional relationship indicated by the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present utility model.

[0046] As Figure 1-8 shown, an automated device for efficient magnetic separation and washing in IVD coating includes a bin body 1 and a bin door 2 hinged to the bin body 1. The bin body 1 is provided with two groups of reactors. The bin body 1 includes an upper space and a lower space, and the upper space and the lower space are separated by an operating table 3 to form independent operating spaces.

[0047] A robot 4 is arranged in the upper space of the bin body 1. The robot 4 is placed on the operating table 3. An ultrasonic degasser 5 is also arranged in the bin body 1. The ultrasonic degasser 5 is placed on one side of the robot 4. A weighing and liquid level device 7 and a pipette support position 8 are respectively arranged between two groups of reactors 6 arranged on the side far from the ultrasonic degasser 5.

[0048] A liquid suction pump 9 and a liquid adding pump 10 are arranged in the lower space of the bin body 1. The solvent in the reactor 6 is pumped into a waste liquid bucket 11 arranged in the lower space of the bin body 1 through the liquid suction pump 9, and the solvent in a reagent bucket 12 arranged in the lower space of the bin body 1 is added into a solution bottle through the liquid adding pump 10.

[0049] An upper / lower material position 13 and a pipette magazine 14 are also arranged in the bin body 1. The pipette magazine 14 is used to place pipettes, and the upper / lower material position 13 is used to store solution bottles.

[0050] The upper / lower material position 13 and the pipette magazine 14 are placed on the operating table and near the bin door 2.

[0051] In order to facilitate the separation and clamping operation of the solution by the robot in the use state, the robot 4 includes a first manipulator 4-1 and a second manipulator 4-2. The first manipulator 4-1 is provided with a claw structure. The claw structure is designed with three-claw hook-shaped claws, and it adopts pneumatic or electric three claws. The second manipulator 4-2 is a pipe orifice tooling manipulator.

[0052] The second manipulator 4-2 includes a tube-holding end 4-2-1 and a tube-withdrawing end 4-2-2. The tube-holding end 4-2-1 adopts a pneumatic or electric two-jaw structure. The tube-withdrawing end 4-2-2 is matched with the connection port end of the pipe orifice tooling 18. The tube-withdrawing end 4-2-2 controls the up and down movement of the withdrawal plate by adopting a pneumatic or electric method. The front end of the withdrawal plate is concave and is matched with the outer diameter of the pipe orifice tooling 18.

[0053] The above-mentioned different manipulators respectively clamp and operate the solution bottle and the straw, which is simple and fast, saving manpower operation.

[0054] In order to fix the solution through the cooperation of the first manipulator and the spring tooling in the use state, a spring tooling is arranged on the first manipulator 4-1. The spring tooling is matched with the jaw structure of the first manipulator 4-1 to fix the bottle cap.

[0055] The spring tooling includes a positioning column 4A. The positioning column 4A is fixedly arranged on the first manipulator 4-1 and is located between the jaw structures of the first manipulator 4-1. A positioning nut is arranged at the end of the positioning column 4A. A spring is sleeved on the positioning column 4A. A star-shaped positioning plate 4B is sleeved on the positioning column 4A, and the star-shaped positioning plate 4B is located at the bottom of the spring.

[0056] With the above-mentioned spring tooling set, during use, the bottle cap is grabbed by the jaws of the first manipulator 4-1, and then fixed by the star-shaped positioning plate 4B against the bottle cap. The star-shaped positioning plate 4B can slide on the positioning column, and the bottle cap is clamped through the action of the spring.

[0057] In order to facilitate storing the solution bottle through the setting of the loading / unloading position 13 in the use state and save the loading operation time, the loading / unloading position 13 is rectangular, and a number of circular accommodating grooves for accommodating the solution bottle are arranged in an array on the loading / unloading position 13.

[0058] In order to facilitate taking out the remaining solution in the bottle body in a short time through the setting of the water absorption structure 15 in the use state, a water absorption structure 15 is arranged adjacent to one side of the ultrasonic degasser 5. The water absorption structure 15 includes a hollow outer frame 15-1 connected to the operating table. The space formed by the outer frame 15-1 is filled with a water absorption sponge 15-2. The water absorption sponge 15-2 on the outer frame 15-1 forms a circular accommodating groove for accommodating the solution bottle.

[0059] The straw support position 8 extends downward to a rectangular groove structure, and the edge of the straw support position 8 is connected to the straw support 17 by bolts.

[0060] The pipe orifice tooling 18 is detachably connected to the straw support 17, and the pipe orifice tooling 18 is inserted and matched with the straw.

[0061] Near the position of the straw holder 8, a hollow waste straw collection tank 19 is also provided. The waste straw collection tank 19 is fixed to the operating table in a rectangular or circular shape.

[0062] In order to facilitate the external discharge of waste liquid by the liquid suction pump in the use state, the liquid suction pump 9 is connected to the waste liquid bucket 11 and the pipe orifice tooling 18 respectively through connecting pipes.

[0063] In order to facilitate the addition of reagents into the solution bottle by the liquid addition pump 10 in the use state, the liquid addition pump 10 is connected to the reagent bucket 12 and the pipe orifice tooling 18 respectively through connecting pipes.

[0064] In order to facilitate movement and positioning in the use state, universal wheels and positioning corners are provided at the bottom of the cabinet 1. A UPS uninterruptible power supply is provided inside the cabinet 1, and the UPS uninterruptible power supply is arranged in the lower space below the loading / unloading position.

[0065] In order to facilitate automatic control in the use state, a robot control box is further included inside the cabinet 1. The robot control box is connected to a control platform connected to the outside of the cabinet 1 through a bracket through a connecting wire;

[0066] The control platform adopts a PIC / single-chip microcomputer controller, and the control platform is electrically connected to the ultrasonic degasser 5 inside the cabinet 1;

[0067] The control platform is electrically connected to the liquid suction pump 9 and the liquid addition pump 10 inside the cabinet 1;

[0068] The control platform is electrically connected to two groups of reactors 6 inside the cabinet 1. The reactor 6 is a separator disclosed in the prior art.

[0069] The utility model realizes automation and is used for producing a magnetic bead coating kit. The biological magnetic beads need to be coated with antibodies of the target extract, so as to extract the target substance in later blood, body fluids and tissues. The separation device is an essential device for coating magnetic beads with antibodies.

[0070] In the working state of the utility model, an operator manually places a bottle filled with a mixed solution at the loading position. The first manipulator identifies the bottle and picks it up, and shakes the bottle evenly for 30 seconds (the time needs to be set). The first manipulator places the bottle into the No. 1 separator (near the rear end of the bin of the first reactor) and puts it down. The solution in the bottle is separated in the separator for about 3 - 10 minutes (the time needs to be set). Then the first manipulator places the bottle into the No. 2 separator (near the position of the suction pipe bin of the second reactor) and puts it down. The solution in the bottle is separated in the separator for about 3 - 10 minutes (the time needs to be set). The second manipulator grabs a straw from the straw bin and inserts the straw into the connecting tooling of the suction pipe of the No. 1 separator through the second manipulator. The second manipulator holds the connecting tooling of the suction pipe and inserts the straw into the center bottom of the separator. The suction pump pumps the solvent in the separator into the waste liquid bucket. The manipulator puts the used straw into the waste straw bin, puts the connecting tooling of the suction pipe back to its original position, takes out the bottle and places it at the working position, grabs a straw from the straw bin, inserts the straw into the connecting tooling of the liquid adding pump, holds the connecting tooling of the liquid adding pump, rotates and rinses the precipitate at the bottle mouth, adds the solvent, holds the bottle, puts it into the ultrasonic cleaner for more than 1 minute (the time needs to be set) and shakes it evenly. Repeat the above operations 3 - 4 times (the number of times needs to be set). Put the completed bottle into the discharging position. When the bottles at the discharging position are full, an alarm will remind the staff to take the materials.

[0071] Among them, during the idle time of the separation in the No. 1 separator, the operation of the No. 2 separator can be carried out. The two workstations are carried out alternately to improve the efficiency.

[0072] In the working state, a disposable straw is used to extract the solvent each time. If there are more than 10 kinds of washing solvents, the injection pipe, hose and joint need to be replaced each time the solvent is changed. To ensure cleanliness, the suction pipe needs to be replaced each time during the 3 - 4 washing processes.

[0073] Among them, a sealed outer cover is added on the operation table, a bin door is set in the front, and side opening doors are set on both the left and right sides of the bin body, with PC board transparent glass, which is convenient for taking materials.

[0074] Among them, the bin body is equipped with a UPS. Even if there is a power failure, it can still run for 1 hour and has an anti - misoperation function. Even if there is a power failure, the gripper will not open and the bottle will not fall.

[0075] For those skilled in the art, it is obvious that the present utility model is not limited to the details of the above-described exemplary embodiments, and the present utility model can be implemented in other specific forms without departing from the spirit or basic characteristics of the present utility model. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting. The scope of the present utility model is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be embraced within the present utility model. Any reference signs in the claims should not be construed as limiting the claims involved.

[0076] In addition, it should be understood that although this specification is described according to embodiments, not every embodiment only contains an independent technical solution. This narrative way of the specification is only for clarity. Those skilled in the art should regard the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. An efficient magnetic separation and washing automated device for IVD coating, comprising a bin body (1) and a bin door (2) hingedly connected to the bin body (1), wherein the bin body (1) is provided with two groups of reactors, characterized in that: The warehouse body (1) comprises two parts, an upper space and a lower space, and the upper space and the lower space are separated by an operating table (3) to form an independent operating space; A robot (4) is arranged in the upper space of the warehouse (1), and the robot (4) is placed on the operating table (3). An ultrasonic degasser (5) is also arranged in the warehouse (1), and the ultrasonic degasser (5) is placed on one side of the robot (4). A weighing and liquid adding position (7) and a pipette support position (8) are respectively arranged between two groups of reactors (6) arranged on the side away from the ultrasonic degasser (5); The lower space of the warehouse body (1) is provided with a liquid suction pump (9) and a liquid adding pump (10), and the solvent in the reactor (6) is extracted by the liquid suction pump (9) and discharged into a waste liquid bucket (11) provided in the lower space of the warehouse body (1), and the solvent in the reagent bucket (12) provided in the lower space of the warehouse body (1) is added into the solution bottle by the liquid adding pump (10); The warehouse body (1) is also provided with an upper / lower material position (13) and a straw material bin (14), and the upper / lower material position (13) and the straw material bin (14) are placed on the operating table and close to the warehouse door (2).

2. The IVD coating high-efficiency magnetic separation and washing automated equipment according to claim 1, characterized in that: The robot (4) comprises a first manipulator (4-1) and a second manipulator (4-2); the first manipulator (4-1) is a clamping claw structure, the clamping claw structure is a three-claw hook-shaped claw design, and adopts a pneumatic or electric three-claw; the second manipulator (4-2) is a pipe opening tooling manipulator; The second manipulator (4-2) comprises a pipe holding end (4-2-1) and a pipe withdrawing end (4-2-2); the pipe holding end (4-2-1) adopts a pneumatic or electric two-claw structure; the pipe withdrawing end (4-2-2) cooperates with the connecting end of the pipe opening tooling (18); the pipe withdrawing end (4-2-2) controls the upward and downward movement of the withdrawing plate by pneumatic or electric means; the front end of the withdrawing plate is concave and cooperates with the outer diameter of the pipe opening tooling (18).

3. The IVD coating high-efficiency magnetic separation and washing automated equipment according to claim 2, characterized in that: The first manipulator (4-1) is provided with a spring fixture, and the spring fixture cooperates with the clamping claw structure of the first manipulator (4-1) to fix the bottle cap; The spring tooling comprises a positioning column (4A), the positioning column (4A) is fixedly arranged on the first manipulator (4-1) and is located between the clamping jaw structures of the first manipulator (4-1), a positioning nut is arranged at the end of the positioning column (4A), a spring is sleeved on the positioning column (4A), a star-shaped positioning plate (4B) is sleeved on the positioning column (4A), and the star-shaped positioning plate (4B) is located at the bottom of the spring.

4. The IVD coating high-efficiency magnetic separation and washing automated equipment according to claim 3, characterized in that: The upper / lower material position (13) is rectangular in shape, and a plurality of circular receiving grooves for receiving solution bottles are arranged in an array on the upper / lower material position (13).

5. The IVD coating high-efficiency magnetic separation and washing automated equipment according to claim 4, characterized in that: A water absorption structure (15) is arranged adjacent to the ultrasonic degasser (5), the water absorption structure (15) comprising a hollow outer frame (15-1) connected to an operating table, the space formed by the outer frame (15-1) being filled with a water absorption sponge (15-2), the water absorption sponge (15-2) on the outer frame (15-1) forming a circular receiving groove for receiving a solution bottle.

6. The IVD coating high-efficiency magnetic separation and washing automated equipment according to claim 5, characterized in that: The straw support position (8) is a rectangular groove structure extending downward, and the edge of the straw support position (8) is connected to the straw support (17) by bolts; The pipette support (17) is detachably connected to a pipe opening tool (18), and the pipe opening tool (18) is plug-fitted with the test tube.

7. The IVD coating high-efficiency magnetic separation and washing automated equipment according to claim 6, characterized in that: A hollow waste straw collection trough (19) is also arranged adjacent to the straw support position (8), and the waste straw collection trough (19) is rectangular or circular and fixed on the operating table.

8. The IVD coating high-efficiency magnetic separation and washing automated equipment according to claim 7, characterized in that: The liquid suction pump (9) is respectively connected to the waste liquid bucket (11) and the pipeline opening tooling (18) via connecting pipelines.

9. The IVD coating high-efficiency magnetic separation and washing automated equipment according to claim 8, characterized in that: The liquid adding pump (10) is connected to the reagent barrel (12) and the pipeline opening tooling (18) respectively through connecting pipelines.

10. The IVD coating high-efficiency magnetic separation and washing automated equipment according to claim 9, characterized in that: The bottom of the warehouse body (1) is provided with a universal wheel and a positioning angle, and the warehouse body (1) is provided with a UPS uninterruptible power supply, and the UPS uninterruptible power supply is arranged in the lower space below the upper / lower material level; The warehouse body (1) also includes a robot control box, and the robot control box is connected to a control platform connected to the outside of the warehouse body (1) via a bracket via a connecting line; The control platform adopts a PIC / single-chip microcomputer controller, and the control platform is electrically connected to the ultrasonic degasser (5) in the warehouse (1); The control platform is electrically connected to the liquid suction pump (9) and the liquid adding pump (10) in the warehouse body (1); The control platform is electrically connected to the two groups of reactors (6) in the warehouse (1).

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