Efficient cellulose saccharification equipment
By using multiple sets of uniform heating pipe networks and pH adjustment tubes in cellulose saccharification equipment, combined with agitating devices and online measurement systems, the problem of uneven distribution of traditional equipment in temperature and pH control is solved, effectively protecting cellulase activity, and enzymatic lysis efficiency and product quality are improved.
Patent Information
- Application Number
- CN202421894835.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-06
- Publication Date
- 2025-06-06
- Estimated Expiration
- 2034-08-06
AI Technical Summary
During the cellulose saccharification process, traditional equipment has problems of uneven distribution in temperature and pH control, which affects the activity of cellulase, increases the cost of enzymes and affects product yield.
A highly efficient cellulose saccharification equipment was designed, using multiple sets of uniform heating pipe networks and pH adjustment tubes, combined with a stirring device and an online measurement system, to achieve precise control of the internal temperature and pH of the equipment.
Through uniform temperature and pH distribution, the activity of cellulase is effectively protected, the enzymatic lysis efficiency is improved, the production cost is reduced, and the product quality is improved.
Smart Images
Figure CN222948363U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of enzymatic saccharification equipment, in particular to a high-efficiency cellulose saccharification equipment. Background Art
[0002] Cellulose saccharification refers to the process of enzymatically hydrolyzing cellulose-rich materials, such as xylose residue, by cellulase to obtain cellulosic glucose. Cellulosic glucose is a non-food bio-based glucose that can effectively solve the problems of "competing with people for food and competing with livestock for feed" and carbon emissions, and can have a positive effect on food security and environmental issues.
[0003] Cellulase is the key to the enzymatic hydrolysis of cellulose and glucose. The activity of cellulase is directly related to the yield and comprehensive cost of the product. Therefore, how to ensure the activity of cellulase in the process of cellulose enzymatic hydrolysis is particularly critical. It has been found that the activity of cellulase is closely related to temperature and pH. After long-term practical exploration, the suitable temperature for cellulase is 52-54°C, and the suitable pH is 4.7-5.3. Therefore, how to strictly control the temperature and pH to ensure the activity of cellulase is a problem faced in actual production.
[0004] In actual production, since the saccharification tank is generally large, uneven temperature and pH distribution often occur during temperature and pH control. For example, when the reaction liquid is heated by the traditional jacket heating method, it is inevitable that the temperature of the bottom material is high and the temperature of the upper material is low. When the pH is adjusted, if a single-point pH control method is used above the tank body, the pH of the upper material will be high and the pH of the lower material will be low. This has a great impact on the activity of cellulase during production, increasing the enzyme cost while also affecting the product yield.
[0005] Therefore, a saccharification device that can more accurately measure and control the pH or temperature inside the device during the cellulose saccharification process, thereby effectively ensuring the activity of cellulase, and ensuring the reaction efficiency, cost and product quality, is a problem that needs to be solved. Utility Model Content
[0006] The purpose of the utility model is to provide a high-efficiency cellulose saccharification device to address the deficiencies of the above-mentioned prior art, so as to achieve more accurate regulation of the temperature or pH in the device to ensure the activity of cellulase, thereby improving the saccharification efficiency.
[0007] To achieve the above-mentioned purpose, the utility model adopts the following technical scheme: a high-efficiency cellulose saccharification equipment, including a saccharification tank, in which a stirring paddle is arranged, a tank body of the saccharification tank is provided with a feed port, a feed funnel, a water inlet, a pH adjustment port, a pH electrode installation port and a temperature probe installation port, a pH electrode is installed through the pH electrode installation port, and a temperature probe is installed through the temperature probe installation port; a heating pipe network is arranged in the tank body, a plurality of heating pipe networks are arranged, each heating pipe network is independent of each other in the tank body and is evenly arranged along the inner periphery of the tank body; a discharge port is arranged at the bottom of the tank body.
[0008] Furthermore, the pH adjustment port is connected to an alkali solution delivery pipe outside the tank body, and a peristaltic pump is arranged on the alkali solution delivery pipe.
[0009] Furthermore, the pH adjustment port is connected to the pH adjustment tube inside the tank body, the lower end of the pH adjustment tube extends to the middle of the tank body, and the probe of the pH electrode is located at the upper layer of the material liquid layer in the tank body.
[0010] Furthermore, there are four heating pipe networks, each of which is provided with a heat medium inlet and a heat medium outlet, the heat medium inlet is connected to the heat medium input pipe, and the heat medium outlet is connected to the heat medium output pipe.
[0011] Furthermore, the heat medium inlet of each heating pipe network is connected to the same heat medium input pipe, and the heat medium outlet is connected to the same heat medium output pipe.
[0012] Furthermore, the discharge port is connected to a discharge pipe, and a control valve and a delivery pump are installed on the discharge pipe.
[0013] Beneficial effects of the utility model:
[0014] 1. It can realize the control of temperature and pH during the enzymatic hydrolysis of cellulose, so that the temperature and pH distribution are more uniform, which plays a positive role in ensuring the activity of cellulose, improving the enzymatic hydrolysis efficiency, reducing production costs and ensuring product quality. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] Figure 1 It is a schematic diagram of the main cross-section of the structure of the utility model;
[0016] Figure 2 This is a schematic diagram of a top view of a saccharification tank of the utility model;
[0017] Figure 3 This utility model Figure 1 Schematic diagram of the cross section of the AA surface.
[0018] The names corresponding to the marks in the figure are:
[0019] 1. Saccharification tank; 11. Feed funnel; 12. Water inlet; 13. pH adjustment port; 131. pH adjustment tube; 14. Discharge port; 15. Feed port; 16. Temperature probe installation port; 17. pH electrode installation port; 2. Stirring device; 21. Stirring motor; 22. Stirring paddle; 3. Water inlet pipe; 4. Alkali solution delivery pipe; 41. Peristaltic pump; 5. Discharge pipe; 51. Control valve; 52. Delivery pump; 6. Heating network; 61. Heat medium inlet; 62. Heat medium outlet; 7. Heat medium input pipe; 8. Heat medium output pipe. DETAILED DESCRIPTION
[0020] The technical solutions in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field belong to the scope of protection of the utility model.
[0021] Embodiments of the present utility model:
[0022] like Figure 1-3 As shown, in the present embodiment, a saccharification tank 1 is provided, a support leg is provided below the saccharification tank 1, a stirring device 2 is provided on the saccharification tank 1, wherein the stirring device 2 includes a stirring motor 21, and the stirring motor 21 is installed on the saccharification tank 1 through a mounting bracket, and the stirring motor 21 is connected to a stirring paddle 22, and the stirring paddle 22 extends to the lower part of the tank body of the saccharification tank 1.
[0023] A feed port 15, a feed funnel 11, a pH adjustment port 13 and a water inlet 12 are provided on the tank body of the saccharification tank 1, wherein the pH adjustment port 13 is connected to an alkali solution delivery pipe 4, the alkali solution delivery pipe 4 is connected to an alkali solution storage tank (sodium hydroxide storage tank), and a peristaltic pump 41 is installed on the alkali solution delivery pipe 4; the pH adjustment port 13 is connected to a pH adjustment pipe 131 in the saccharification tank 1, and the pH adjustment pipe 131 extends to the middle position of the tank body of the saccharification tank 1; the water inlet 12 is connected to the water inlet pipe 3, and a temperature probe installation port 16 and a pH electrode installation port 17 are provided on the tank body of the saccharification tank 1, a temperature probe is installed through the temperature probe installation port 16, and a pH electrode is installed through the pH electrode installation port 17.
[0024] A discharge port 14 is provided at the bottom of the saccharification tank 1 , and the discharge port 14 is connected to a discharge pipe 5 , on which a control valve 51 and a delivery pump 52 are installed.
[0025] A heating pipe network 6 is arranged in the tank body of the saccharification tank 1, wherein four heating pipe networks 6 are provided and are evenly distributed around the inner periphery of the tank body, the heat medium inlets 61 of the heating pipe network 6 are respectively connected to the heat medium input pipe 7, and the heat medium outlets 62 of the heating pipe network 6 are respectively connected to the heat medium output pipe 8.
[0026] The principle of this utility model is:
[0027] The utility model adjusts the pH of the saccharification tank 1 during enzymatic hydrolysis through the pH regulating tube 131, and uniformly heats the tank through the heating pipe network 6, thereby ensuring the activity of the enzyme and thus ensuring that the enzymatic hydrolysis saccharification reaction proceeds stably and efficiently.
[0028] During the reaction, a proper amount of water is added into the tank through the water inlet 12, and then materials are added into the tank through the feed inlet 15, wherein the materials are xylose residues, etc., and stirring is started during the addition process to ensure stable addition of the materials.
[0029] After the material is added, the material in the tank is heated by the heating pipe network 6. The heat medium in the process can be hot water, steam, etc., and the temperature is controlled by the temperature probe (the temperature probe transmits a signal to the on-site PLC, and the on-site PLC controls the flow of the heat medium to ensure the stability of the temperature. The electric valve and flow meter involved in the process are not shown in the attached figure). When the temperature is appropriate (53±1°C), alkali solution is added to adjust the pH of the material. During the process, the pH electrode is used for online measurement of the pH and the measurement results are transmitted to the on-site PLC. The on-site PLC regulates the peristaltic pump 41 to achieve closed-loop control of the pH. During the process, the pH is controlled at 4.7-5.3.
[0030] After the above-mentioned regulation is completed, cellulase is added to the tank through the feed funnel 11, so that under stable temperature and pH conditions, the cellulase can ensure high activity and achieve enzymatic hydrolysis of cellulose in the material, thereby producing glucose; after the reaction is completed, the liquid is pumped to the next stage through the delivery pump 52 for treatment, such as filter pressing and crystallization, to obtain glucose.
[0031] In the utility model, since the saccharification tank 1 is generally large, directly adding alkali solution into the tank body requires time for the alkali solution to disperse, thus causing inaccurate regulation. In the utility model, the alkali solution adding position, i.e., the outlet of the pH regulating tube 131, is arranged in the middle of the tank body, and the pH electrode is installed in the upper layer of the material liquid layer in the tank body, which can effectively avoid the occurrence of large regulation errors; at the same time, multiple groups of evenly distributed heating pipe networks 6 are used to achieve uniform heating of the material in the tank body, which can effectively avoid the temperature difference between the upper and lower layers of the material liquid caused by traditional jacket heating and the like.
Claims
1. A high-efficiency cellulose saccharification device, comprising a saccharification tank (1), wherein a stirring paddle (22) is arranged in the saccharification tank (1), characterized in that: The saccharification tank (1) is provided with a feed port (15), a feed funnel (11), a water inlet (12), a pH adjustment port (13), a pH electrode installation port (17) and a temperature probe installation port (16) on its tank body. A pH electrode is installed through the pH electrode installation port (17), and a temperature probe is installed through the temperature probe installation port (16). A heating pipe network (6) is provided in the tank body. A plurality of heating pipe networks (6) are provided. Each heating pipe network (6) is independent of each other in the tank body and is evenly arranged along the inner periphery of the tank body. A discharge port (14) is provided at the bottom of the tank body.
2. The high-efficiency cellulose saccharification equipment according to claim 1, characterized in that: The pH adjustment port (13) is connected to the alkali solution delivery pipe (4) outside the tank body, and a peristaltic pump (41) is arranged on the alkali solution delivery pipe (4).
3. The high-efficiency cellulose saccharification equipment according to claim 2, characterized in that: The pH adjustment port (13) is connected to a pH adjustment tube (131) inside the tank body, the lower end of the pH adjustment tube (131) extends to the middle of the tank body, and the probe of the pH electrode is located at the upper layer of the liquid layer in the tank body.
4. The high-efficiency cellulose saccharification equipment according to claim 1, characterized in that: There are four heating pipe networks (6), each of which is provided with a heat medium inlet (61) and a heat medium outlet (62). The heat medium inlet (61) is connected to the heat medium input pipe (7), and the heat medium outlet (62) is connected to the heat medium output pipe (8).
5. The high-efficiency cellulose saccharification equipment according to claim 4, characterized in that: The heat medium inlet (61) of each heating pipe network (6) is connected to the same heat medium input pipe (7), and the heat medium outlet (62) is connected to the same heat medium output pipe (8).
6. The high-efficiency cellulose saccharification equipment according to claim 1, characterized in that: The discharge port (14) is connected to a discharge pipe (5), and a control valve (51) and a delivery pump (52) are installed on the discharge pipe (5).