Structure capable of being used for loading flaky carrier on bioreactor tank body

Abstract

The utility model relates to the technical field of cell culture, and particularly discloses a structure capable of being used for loading a flaky carrier on a bioreactor tank body, which comprises a support frame, a tank body reactor and a carrier plate, the reactor tank cover is mounted on the outer wall of the top of the tank reactor, a plurality of mounting ports are inserted into the outer wall of the top of the reactor tank cover, and sealing plates are mounted in the mounting ports; a top fixed clamping jaw is mounted in the center of the outer wall of the top of the tank reactor; according to the utility model, the disposable membrane bracket and the sheet-shaped carrier mounting chamber are used as disposable appliances and can be directly mounted inside the tank reactor, so that the suspension culture reactor is changed into an adherent culture reactor, a special adherent reactor does not need to be purchased, and the disposable membrane bracket and the sheet-shaped carrier mounting chamber are convenient to mount and dismount.

Description

Technical Field

[0001] The utility model belongs to the technical field of cell culture, and particularly relates to a structure that can be used for loading sheet carriers in a bioreactor tank body. Background Art

[0002] Large-scale cell culture processes in the field of biopharmaceuticals can be divided into suspension culture and adherent culture. Suspension culture is convenient to operate, but the cells need to be acclimated to suspension, which is a time-consuming and laborious process. Moreover, there is a possibility of cell atavism, and the shear force on the suspension-cultured cells is large, which will affect the cell viability and thus the yield of the final product. Adherent culture requires providing a surface for the cells to attach and grow. Small-scale adherent cell culture can be carried out through cell culture flasks and culture dishes; large-scale adherent cell culture requires the use of cell factories or bioreactors. Cell factories cover a large area, have many manual operation links, high pollution risks, and lack precise control of various parameters during the culture process. In addition, when carrying out large-scale production, the application of cell factories is more inconvenient.

[0003] That is, most of the devices are used for cell culture in the form of suspension culture, which is also the mainstream culture method. When adherent culture is required, a dedicated reactor for adherent culture needs to be selected, which not only occupies more space but also increases the use cost.

[0004] Therefore, those skilled in the art have proposed a structure that can be used for loading sheet carriers in a bioreactor tank body to solve the above-mentioned problems. Content of the Utility Model

[0005] The purpose of the utility model is to provide a structure that can be used for loading sheet carriers in a bioreactor tank body to solve the problems raised in the above background art.

[0006] To achieve the above purpose, the utility model provides the following technical solutions:

[0007] A structure that can be used for loading sheet carriers in a bioreactor tank body, comprising:

[0008] A support frame, inside which a tank reactor is installed;

[0009] A reactor tank cover, which is installed on the outer wall of the top of the tank reactor. A plurality of mounting ports are inserted on the outer wall of the top of the reactor tank cover, and sealing plates are installed inside the mounting ports;

[0010] Sheet carrier installation chamber, a top fixing jaw is installed at the center position of the outer wall of the top of the tank reactor. A stirring motor drive shaft is vertically inserted into the inner wall of the top fixing jaw. The bottom end of the stirring motor drive shaft is located inside the tank reactor. A hollow cylinder is sleeved on the circumferential outer wall of the stirring motor drive shaft. The top end of the hollow cylinder is installed on the bottom outer wall of the top fixing jaw. Disposable membrane supports are sleeved on the circumferential outer wall of the hollow cylinder near the upper and lower positions. The sheet carrier installation chamber is installed on the outer wall of the disposable membrane support. Multiple layers of sheet carrier membranes are wound around the circumferential outer wall of the hollow cylinder. There is a gap between adjacent layers of the sheet carrier membranes. The top of the sheet carrier installation chamber is designed to be open. A through hole is opened at the center position of the bottom outer wall of the sheet carrier installation chamber.

[0011] Preferably, a flow equalizing plate is horizontally installed near the middle position inside the sheet carrier installation chamber.

[0012] Preferably, a propeller is installed on the circumferential outer wall of the stirring motor drive shaft near the lower position. A spoiler is installed outside the propeller. The propeller is located below the flow equalizing plate, and the sheet carrier membrane is located above the flow equalizing plate.

[0013] Preferably, a sampling hole is opened on one side of the outer wall of the top of the reactor tank cover, and an orifice plate is installed on the inner wall of the sampling hole.

[0014] Compared with the prior art, the beneficial effects of the present utility model are:

[0015] (1) The utility model is provided with a disposable membrane support and a sheet carrier installation chamber. The disposable membrane support and the sheet carrier installation chamber, as disposable appliances, can be directly installed inside the tank reactor, thereby turning the suspension culture reactor into an adherent culture reactor without the need to purchase a dedicated adherent reactor. Moreover, the disposable membrane support and the sheet carrier installation chamber are convenient for installation and disassembly. The sheet carrier membrane is installed on the outer side of the hollow cylinder inside the sheet carrier installation chamber and wound into a cylinder. The culture medium is fed into the interior of the tank reactor. The top end of the transmission shaft of the stirring motor is connected to the motor, and the rotation of the stirring motor transmission shaft is driven by the motor, thereby driving the rotation of the bottom propeller, so that the culture medium circulates inside the tank reactor. The propeller and the spoiler generate a liquid flow moving upward from the bottom, and the seed cells can be quickly and evenly inoculated onto the sheet carrier membrane by means of this liquid flow. With this structure, the cell perfusion culture process can be easily realized, further reducing the material cost of cell culture and increasing the product yield. When cell sampling is required, a slender sampling strip made of the same material as the sheet carrier is inserted into the gap between adjacent layers of the sheet carrier membrane, and one end of the sampling strip is longer, with a height reaching near the sampling hole. The sampling strip can be pulled out through the sampling hole using sterile forceps for aseptic sampling, reducing the sampling error and solving the technical problem of difficult sampling in sheet carrier cell culture. Sampling can be carried out at any time during the production cycle of cell culture to detect the cell state. During the stirring process, the liquid inside the tank reactor enters through the through holes at the bottom of the sheet carrier installation chamber, and under the action of the propeller and the spoiler, it moves upward through the flow equalizing plate, then reaches the position of the sheet carrier membrane, and forms a waterfall flow from the top of the sheet carrier membrane and flows back down to the interior of the reactor tank body, and the cells adhere to the surface of the sheet carrier membrane. Description of the Drawings

[0016] Figure 1 is the overall structural schematic diagram of the present utility model;

[0017] Figure 2 is the internal structural schematic diagram of the device of the present utility model;

[0018] Figure 3 is the unfolded structural schematic diagram of the device of the present utility model;

[0019] Figure 4 is the sectional structural schematic diagram after the sheet carrier membrane of the present utility model is installed inside the sheet carrier installation chamber;

[0020] In the figure: 1, tank reactor; 2, propeller; 3, sheet carrier installation chamber; 4, top fixed jaw; 5, reactor tank cover; 6, hollow cylinder; 7, stirring motor transmission shaft; 8, spoiler; 9, orifice plate; 10, support frame; 11, sampling hole; 12, disposable membrane support; 13, through hole; 14, flow equalizing plate. Detailed Description of the Invention

[0021] The following will clearly and completely describe the technical solutions in the embodiments of the present utility model in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present utility model without creative efforts shall fall within the protection scope of the present utility model.

[0022] Please refer to Figures 1-4 As shown, a structure for loading sheet carriers in a bioreactor tank includes:

[0023] A support frame 10, inside which a tank reactor 1 is installed;

[0024] A reactor tank cover 5, which is installed on the outer wall of the top of the tank reactor 1. On the outer wall of the top of the reactor tank cover 5, a plurality of mounting ports are inserted, and sealing plates are installed inside the mounting ports;

[0025] A sheet carrier installation chamber 3, a top fixing jaw 4 is installed at the center of the outer wall of the top of the tank reactor 1. A stirring motor transmission shaft 7 is vertically inserted into the inner wall of the top fixing jaw 4. The bottom end of the stirring motor transmission shaft 7 is located inside the tank reactor 1. A hollow cylinder 6 is sleeved on the circumferential outer wall of the stirring motor transmission shaft 7. The top end of the hollow cylinder 6 is installed on the bottom outer wall of the top fixing jaw 4. Disposable membrane supports 12 are sleeved on the circumferential outer wall of the hollow cylinder 6 near the upper and lower positions. The sheet carrier installation chamber 3 is installed on the outer wall of the disposable membrane support 12. A multi-layer sheet carrier membrane is wound around the circumferential outer wall of the hollow cylinder 6, and there are gaps between adjacent layers of the sheet carrier membrane. The top of the sheet carrier installation chamber 3 is designed to be open, and a through hole 13 is opened at the center of the bottom outer wall of the sheet carrier installation chamber 3.

[0026] As can be seen from the above, the disposable membrane support 12 and the sheet carrier installation chamber 3 are installed inside the tank reactor 1, converting the suspension culture device into an adherent culture reactor. The feeding pipe is inserted through the mounting port at the top of the reactor tank cover 5 to feed the culture medium into the tank reactor 1. Then, the top end of the stirring motor transmission shaft 7 is connected to the motor, and the motor drives the rotation of the stirring motor transmission shaft 7, so that the culture medium circulates inside the tank reactor 1. Then, driven by the stirring motor transmission shaft 7, the culture medium enters the inside of the sheet carrier installation chamber 3. When the liquid passes through the sheet carrier membrane, cells adhere to the surface of the sheet carrier membrane, and thus adherent culture begins.

[0027] Specifically, a flow equalizing plate 14 is horizontally installed near the middle inside the sheet carrier installation chamber 3, and the sheet carrier membrane is located above the flow equalizing plate 14.

[0028] As can be seen from the above, the liquid is stably transported upward through the flow equalizing plate 14.

[0029] Specifically, a propeller 2 is installed on the outer circumference of the stirring motor drive shaft 7 near the lower part, a spoiler 8 is installed outside the propeller 2, and the propeller 2 is located below the flow equalizing plate 14.

[0030] As can be seen from the above, the stirring motor drive shaft 7 drives the propeller 2 and the spoiler 8 to rotate. The propeller 2 and the spoiler 8 generate a liquid flow moving upward. The seed cells can be quickly and evenly inoculated onto the sheet carrier membrane by means of this liquid flow. With the structures of the disposable membrane support 12 and the sheet carrier installation chamber 3, the cell perfusion culture process can be easily realized, further reducing the material cost of cell culture and increasing the product yield. When cell sampling is required, a slender sampling strip made of the same material as the sheet carrier is inserted into the gap between adjacent layers of the sheet carrier membrane, and one end of the sampling strip is longer, with a height reaching near the sampling hole 11. The sampling strip can be pulled out through the sampling hole 11 using sterile forceps for aseptic sampling, reducing sampling errors and solving the technical problem of difficult sampling in sheet carrier cell culture. Sampling can be carried out at any time during the production cycle of cell culture to detect the cell state.

[0031] Specifically, a sampling hole 11 is opened on one side of the outer wall of the reactor tank cover 5, and an orifice plate 9 is installed on the inner wall of the sampling hole 11.

[0032] As can be seen from the above, the orifice plate 9 is removed, and sampling can be carried out at any time during the production cycle of cell culture through the sampling hole 11 to detect the cell state.

[0033] Working principle: The disposable membrane support 12 and the sheet carrier installation chamber 3 are used as disposable appliances and are directly installed inside the tank reactor 1, thus turning the suspension culture reactor into an adherent culture reactor without the need to purchase a dedicated adherent reactor. Moreover, the disposable membrane support 12 and the sheet carrier installation chamber 3 are convenient for installation and disassembly. The sheet carrier membrane is installed on the outside of the hollow cylinder 6 inside the sheet carrier installation chamber 3 and wound into a cylinder. The feeding pipe is inserted through the installation opening at the top of the reactor tank cover 5 to send the culture medium into the inside of the tank reactor 1. Then, the top end of the stirring motor transmission shaft 7 is connected to the motor, and the rotation of the stirring motor transmission shaft 7 is driven by the motor, so that the culture medium circulates inside the tank reactor 1. Then, driven by the stirring motor transmission shaft 7, the culture medium enters the inside of the sheet carrier installation chamber 3. When the liquid passes through the sheet carrier membrane, cells adhere to the surface of the sheet carrier membrane, thus completing adherent culture. When cell sampling is required, the orifice plate 9 is removed. A slender sampling strip made of the same material as the sheet carrier is inserted into the gap between adjacent layers of the sheet carrier membrane, and one end of the sampling strip is longer, with a height reaching near the sampling hole 11. The sampling strip can be pulled out through the sampling hole 11 using sterile forceps for sterile sampling, reducing sampling errors and solving the technical problem of difficult sampling in sheet carrier cell culture. Sampling can be carried out at any time during the production cycle of cell culture through the sampling hole 11 to detect the cell state.

[0034] Although the embodiments of the present invention have been shown and described, those of ordinary skill in the art can understand that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A structure that can be used for loading a sheet carrier on a bioreactor tank, characterized in that: include: A support frame (10), wherein a tank reactor (1) is installed inside the support frame (10); A reactor tank cover (5), the reactor tank cover (5) being mounted on the top outer wall of the tank reactor (1), the top outer wall of the reactor tank cover (5) being provided with a plurality of mounting openings, and a sealing plate being mounted inside the mounting openings; A sheet carrier installation chamber (3), a top fixed clamp (4) is installed at the center position of the top outer wall of the tank reactor (1), a stirring motor drive shaft (7) is vertically inserted on the inner wall of the top fixed clamp (4), the bottom end of the stirring motor drive shaft (7) is located inside the tank reactor (1), a hollow cylinder (6) is sleeved on the circumferential outer wall of the stirring motor drive shaft (7), the top end of the hollow cylinder (6) is installed on the bottom outer wall of the top fixed clamp (4), and a disposable membrane bracket (12) is sleeved on the circumferential outer wall of the hollow cylinder (6) near the upper and lower positions, the sheet carrier installation chamber (3) is installed on the outer wall of the disposable membrane bracket (12), and a plurality of layers of sheet carrier membranes are wound on the circumferential outer wall of the hollow cylinder (6), and gaps are left between adjacent layers of the sheet carrier membrane. The top of the sheet carrier installation chamber (3) is designed to be open, and a through hole (13) is opened at the center of the bottom outer wall of the sheet carrier installation chamber (3).

2. A structure for loading sheet carriers on a bioreactor tank according to claim 1, characterized in that: A flow balancing plate (14) is horizontally installed near the middle of the sheet carrier installation chamber (3), and the sheet carrier membrane is located above the flow balancing plate (14).

3. A structure for loading sheet carriers on a bioreactor tank according to claim 2, characterized in that: A propeller (2) is installed near the bottom of the circumferential outer wall of the stirring motor transmission shaft (7), a spoiler (8) is installed on the outer side of the propeller (2), and the propeller (2) is located below the flow equalizing plate (14).

4. The structure for loading a sheet carrier on a bioreactor tank according to claim 1, characterized in that: A sampling hole (11) is opened on one side of the outer wall at the top of the reactor tank cover (5), and a perforated plate (9) is installed on the inner wall of the sampling hole (11).

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