Double-rotating-disc type pipetting and centrifuging integrated device for flaking

Abstract

The double-rotating-disc type pipetting and centrifuging integrated device comprises a bottom frame, the bottom frame is provided with a first rotating disc and a second rotating disc which are coaxially arranged and can rotate, the first rotating disc is provided with a plurality of sampling bottle containing holes in the circumferential direction, the sampling bottle containing holes are used for containing sampling bottles, and the second rotating disc is provided with a plurality of flaking bin containing grooves in the circumferential direction. The bottom frame is further provided with a multi-axis moving mechanism, the multi-axis moving mechanism is provided with a liquid transferring device, and the liquid transferring device is used for transferring liquid between the sampling bottles and the flaking bins, so that the problems that a traditional flaking machine is large in occupied area and high in efficiency due to the fact that the positions of centrifugal stations and liquid transferring stations are distributed loosely are solved. And the structure is not compact enough.

Description

Technical Field

[0001] The utility model relates to the field of cell detection, in particular to a dual-rotary-table pipetting and centrifuging integrated device for slide preparation. Background Technique

[0002] Liquid-based thin-layer cytology test is a cervical cancer screening technique that is widely used at present. Cervical exfoliated cells are put into a sampling bottle containing cell preservation solution, and the cells are made into a thin layer and fixed on a glass slide through centrifugation and other methods. After staining and covering the slide, the test can be completed. This cervical cancer screening method has a series of advantages such as low price, accurate results, and fast detection time. Since centrifugation usually requires a dedicated slide preparation chamber, this involves the process of transferring the cell sample from the sampling bottle to the slide preparation chamber.

[0003] In traditional slide preparation stations, pipetting and centrifugation belong to two different upstream and downstream workstations, and they are usually separately arranged in terms of layout. Since the centrifugation process takes a long time in the cycle time, the centrifugation mechanism must be set as a rotary table type, and the slide preparation chambers are centrifuged in batches to evenly distribute the cycle time. Therefore, the centrifugation mechanism itself requires a large area. Coupled with the pipetting workstation and the sampling bottle buffer workstation upstream of the pipetting workstation, the overall equipment occupies a large area and the structure is not compact enough. Content of the Utility Model

[0004] The utility model provides a dual-rotary-table pipetting and centrifuging integrated device for slide preparation, which solves the problem that the positions of the centrifugation workstation and the pipetting workstation in traditional slide preparation machines are relatively loose, resulting in a large floor area and an insufficiently compact structure of the slide preparation machine.

[0005] To solve the above technical problems, the technical solution adopted by the utility model is: a dual-rotary-table pipetting and centrifuging integrated device for slide preparation, including a bottom frame. On the bottom frame, a first rotary table and a second rotary table which are coaxially arranged and rotatable are provided. The first rotary table is provided with a plurality of sampling bottle placement holes along the circumferential direction, and the sampling bottle placement holes are used for placing sampling bottles. The second rotary table is provided with a plurality of slide preparation chamber placement grooves along the circumferential direction, and the slide preparation chamber placement grooves are used for placing slide preparation chambers. The bottom frame is further provided with a multi-axis moving mechanism, and the multi-axis moving mechanism is provided with a pipetting device, and the pipetting device is used for transferring liquid between the sampling bottle and the slide preparation chamber.

[0006] In a preferred solution, the bottom frame includes a bottom plate and an upper plate. A first rotary motor is provided on the bottom plate, and the first rotary motor drives the first rotary table to rotate. A second rotary motor is provided on the upper plate, and the second rotary motor drives the second rotary table to rotate.

[0007] In a preferred embodiment, the upper plate is provided with a first through hole and a second through hole. The pipetting device includes a pipette tube. The inner diameters of the first through hole and the second through hole are greater than the outer diameter of the pipette tube. The first through hole is aligned with the slide-making chamber below, and the second through hole is aligned with the sampling bottle below. The pipette tube is inserted into the slide-making chamber or the sampling bottle from below through the first through hole or the second through hole.

[0008] In a preferred embodiment, the multi-axis moving mechanism includes a first linear module, which is connected to the upper plate. A second linear module is provided on the first linear module, and a third linear module is provided on the second linear module. The displacement directions of the sliding plates of the first linear module, the second linear module, and the third linear module are perpendicular to each other in pairs. The pipetting device is connected to the sliding plate of the third linear module. The upper plate is further provided with a pipette tube storage bin, which includes a plurality of pipette tube placement holes arranged in rows and columns for placing pipette tubes.

[0009] In a preferred embodiment, the pipetting device includes a base block. A tube clamp is provided at the lower end of the base block. The tube clamp includes a cylindrical tube. The outer diameter of the outer wall of the cylindrical tube is smaller than the inner diameter of the pipette tube. One end of the inner cavity of the cylindrical tube is closed. An opening is provided at one end of the cylindrical tube close to the tube clamp. A plurality of telescopic tongues are provided along the circumferential direction at the open end of the cylindrical tube. A wedge surface is provided on the inner side of each telescopic tongue close to the inside. A conical top block is provided in the cylindrical tube. A spring is provided at one end of the conical top block, and the spring abuts against the closed end of the cylindrical tube. The wedge surfaces of the telescopic tongues abut against the conical surface of the conical top block. An annular arc groove is provided on the outer wall of the telescopic tongue, and a flange is provided on the inner wall of the upper port of the pipette tube. The annular arc groove is stuck on the flange. A filling block is provided between the telescopic tongues in the cylindrical tube. A second air passage is provided in the base block, and one end of the second air passage communicates with the open end of the cylindrical tube.

[0010] In a preferred embodiment, a sealing block is provided at the lower end of the base block to block the upper port of the pipette tube. A second ventilation hole is provided in the center of the sealing block, and the second ventilation hole communicates with the second air passage. The sealing block is further provided with a plurality of first ventilation holes arranged axially. The base block is provided with a ventilation ring groove, and each first ventilation hole communicates through the ventilation ring groove. A first air passage is further provided in the base block, and one end of the first air passage communicates with the ventilation ring groove.

[0011] In a preferred embodiment, a central fixing plate is further provided. The first rotating motor is installed on the central fixing plate. An intermediate connecting ring is provided between the central fixing plate and the first turntable. The intermediate connecting ring is lower than the central fixing plate and the first turntable. A plurality of first connecting columns are provided along the circumferential direction at the lower end of the first turntable, and a plurality of second connecting columns are provided along the circumferential direction at the lower end of the central fixing plate. The lower ends of the first connecting columns and the second connecting columns are connected to the intermediate connecting ring.

[0012] The beneficial effects of the present utility model are as follows: The sampling bottle placement station is concentrically arranged with the centrifugal turntable, saving planar position space, making the structure of the tablet making machine more compact, and facilitating the miniaturization of the equipment; A transfer mechanism is arranged above the centrifugal turntable. The pipette is used to transfer the cell sample in the sampling bottle to the tablet making chamber, and at the same time, the pipette can also be used to suck out the waste liquid after centrifugation, eliminating the need to set up a turning mechanism for the tablet making chamber and saving costs. Description of the Drawings

[0013] The present utility model will be further described below in conjunction with the drawings and embodiments.

[0014] Figure 1 It is a schematic diagram of the inside of the pipetting and centrifuging integrated device.

[0015] Figure 2 It is a top view schematic diagram of the pipetting and centrifuging integrated device.

[0016] Figure 3 It is an oblique view structural diagram of the pipetting and centrifuging integrated device.

[0017] Figure 4 It is a structural diagram of the peripheral avoidance type first turntable.

[0018] Figure 5 It is a schematic diagram of the centrifugal state of the tablet making chamber.

[0019] Figure 6 It is a schematic diagram of the pipetting device.

[0020] Figure 7 It is a cross-sectional view at the pipette clamp.

[0021] Figure 8 It is a schematic diagram of the distribution of the telescopic tongues of the pipette clamp.

[0022] In the figure: bottom frame 1; bottom plate 101; upper plate 102; first through hole 103; second through hole 104; first turntable 2; first rotating motor 201; sampling bottle placement hole 202; first connecting column 203; central fixing plate 204; second connecting column 205; intermediate connecting ring 206; second turntable 3; second rotating motor 301; tablet making chamber placement groove 302; tablet making chamber resting plate 303; resting plate rotating shaft 304; lower extending part 305; multi-axis moving mechanism 4; first linear module 401; second linear module 402; third linear module 403; pipetting device 5; pipette 501; flange 502; stop shoulder 503; sealing block 504; first ventilation hole 505; ventilation ring groove 506; first air passage 507; second ventilation hole 508; second air passage 509; base block 510; pipette clamp 511; cylinder 512; telescopic tongue 513; cone top block 514; spring 515; clearance part 516; filling block 517; pipette storage bin 6; pipette placement hole 601. Detailed Embodiment

[0023] As shown in Figure 1-8 In [[ID=]], a double - turntable pipetting and centrifuging integrated device for slide preparation includes a bottom frame 1. On the bottom frame 1, a first turntable 2 and a second turntable 3 which are coaxially arranged and rotatable are provided. The first turntable 2 is provided with a plurality of sampling bottle placement holes 202 along the circumferential direction. The sampling bottle placement holes 202 are used for placing sampling bottles. The second turntable 3 is provided with a plurality of slide preparation chamber placement grooves 302 along the circumferential direction. The slide preparation chamber placement grooves 302 are used for placing slide preparation chambers. The bottom frame 1 is further provided with a multi - axis moving mechanism 4. The multi - axis moving mechanism 4 is provided with a pipetting device 5. The pipetting device 5 is used for transferring liquid between the sampling bottles and the slide preparation chambers.

[0024] The number of the sampling bottle placement holes 202 and the slide preparation chamber placement grooves 302 is equal and they are both evenly distributed along the circumferential direction. Each sampling bottle corresponds to a slide preparation chamber on the radial line.

[0025] In a preferred solution, the bottom frame 1 includes a bottom plate 101 and an upper plate 102. A first rotating motor 201 is provided on the bottom plate 101. The first rotating motor 201 drives the first turntable 2 to rotate. A second rotating motor 301 is provided on the upper plate 102. The second rotating motor 301 drives the second turntable 3 to rotate.

[0026] Both the first rotating motor 201 and the second rotating motor 301 are motors with angle encoders, and the circumferential angles of the first turntable 2 and the second turntable 3 can be adjusted.

[0027] The second rotating motor 301 can adopt a high - speed torque motor to ensure the centrifugal rotation speed.

[0028] In a preferred solution, the upper plate 102 is provided with a first through - hole 103 and a second through - hole 104. The pipetting device 5 includes a pipetting tube 501. The inner diameters of the first through - hole 103 and the second through - hole 104 are larger than the outer diameter of the pipetting tube 501. The first through - hole 103 is aligned with the slide preparation chamber below, and the second through - hole 104 is aligned with the sampling bottle below. The pipetting tube 501 is inserted into the slide preparation chamber or the sampling bottle from the first through - hole 103 or the second through - hole 104 downward.

[0029] The first through - hole 103 and the second through - hole 104 are respectively on the diameter lines of the distribution of the slide preparation chamber and the sampling bottle. When liquid needs to be aspirated, only the second turntable 3 and the first turntable 2 need to be rotated below the upper plate 102.

[0030] In a preferred solution, the multi-axis moving mechanism 4 includes a first linear module 401, the first linear module 401 is connected to the upper plate 102, a second linear module 402 is provided on the first linear module 401, and a third linear module 403 is provided on the second linear module 402. The sliding plate displacement directions of the first linear module 401, the second linear module 402, and the third linear module 403 are perpendicular to each other in pairs. The pipetting device 5 is connected to the sliding plate of the third linear module 403. A pipette storage bin 6 is further provided on the upper plate 102. The pipette storage bin 6 includes a plurality of pipette placement holes 601 arranged in rows and columns for placing pipettes 501.

[0031] A stop shoulder 503 is provided on the outer side of the open end of the pipette 501, which can be stuck at the port of the pipette placement hole 601 to prevent the liquid suction port at the lower end of the pipette 501 from contacting the tabletop.

[0032] The first linear module 401, the second linear module 402, and the third linear module 403 all adopt a lead screw-guide rail module driven by a servo motor, with good integration. The three form a Cartesian structure, which is convenient for picking and placing the pipette 501 and pipetting into the slide preparation chamber or the sampling bottle.

[0033] The multi-axis moving mechanism 4 drives the pipetting device 5 to load a clean pipette 501 from the pipette placement hole 601 and displace it above the second through hole 104. The first turntable 2 and the second turntable 3 rotate so that the sampling bottle and the slide preparation chamber are respectively aligned with the upper second through hole 104 and the first through hole 103. The third linear module 403 drives the pipette 501 to descend, pass through the second through hole 104 and insert into the sampling bottle to aspirate the sample. The third linear module 403 drives the pipette 501 to move up, and the second linear module 402 drives the pipette 501 to reach above the first through hole 103. The pipette 501 is inserted downward into the slide preparation chamber to inject the sample. The multi-axis moving mechanism 4 drives the pipette 501 to reach the waste bin on one side of the bottom frame 1 and discard the pipette 501.

[0034] Subsequently, the second rotary motor 301 drives the second turntable 3 to rotate rapidly for centrifugation. A slide preparation chamber support plate 303 is provided at the slide preparation chamber placement groove 302. The slide preparation chamber is stably fixed on the slide preparation chamber support plate 303 to ensure safety during centrifugation. Rotating shafts 304 of the slide preparation chamber support plate 303 are provided in the middle of both sides of the slide preparation chamber support plate 303. A downward extension 305 is provided on one side of the slide preparation chamber support plate 303 close to the rotating shaft of the second turntable 3, so the center of gravity is biased backward. The slide preparation chamber includes a liquid injection cylinder and a slide below. During centrifugation, the slide preparation chamber will tilt so that the slide at the bottom of the slide preparation chamber is biased outward, so that the sample substance adheres to the slide under the action of centrifugal force. After centrifugation is completed, the multi-axis moving mechanism 4 drives the pipetting device 5 to load a new pipette 501 again, aspirate the waste liquid in the slide preparation chamber, discharge it to the waste liquid tank, and discard the pipette 501 into the waste bin. Clean the slide preparation chamber and take out the slide for the next staining process.

[0035] In a preferred embodiment, the pipetting device 5 includes a base block 510. A pipe clamp 511 is provided at the lower end of the base block 510. The pipe clamp 511 includes a cylinder 512. The outer diameter of the cylinder 512 is smaller than the inner diameter of the pipette 501. One end of the inner cavity of the cylinder 512 is closed. An opening is provided at one end of the cylinder 512 close to the pipe clamp 511. A plurality of telescopic tongues 513 are provided along the circumferential direction at the opening end of the cylinder 512. A wedge surface is provided on the inner side of the telescopic tongue 513. A cone top block 514 is provided in the cylinder 512. A spring 515 is provided at one end of the cone top block 514. The spring 515 abuts against the closed end of the cylinder 512. The wedge surfaces of the telescopic tongues 513 abut against the conical surface of the cone top block 514. An annular arc groove is provided on the outer wall of the telescopic tongue 513. A flange 502 is provided on the inner wall of the upper port of the pipette 501. The annular arc groove is stuck on the flange 502. A filling block 517 is provided between the telescopic tongues 513 in the cylinder 512. A second air passage 509 is provided in the base block 510. One end of the second air passage 509 communicates with the opening end of the cylinder 512.

[0036] In a preferred embodiment, a sealing block 504 is provided at the lower end of the base block 510. The sealing block 504 plugs the upper port of the pipette 501. A second ventilation hole 508 is provided in the center of the sealing block 504. The second ventilation hole 508 communicates with the second air passage 509. The sealing block 504 is also provided with a plurality of first ventilation holes 505 arranged axially. The base block 510 is provided with a ventilation ring groove 506. Each first ventilation hole 505 communicates through the ventilation ring groove 506. A first air passage 507 is also provided in the base block 510. One end of the first air passage 507 communicates with the ventilation ring groove 506.

[0037] Quick connectors are provided at one ends of the first air passage 507 and the second air passage 509 for connecting a control valve of an external air circuit.

[0038] The filling block 517 can reduce the gap between the telescopic tongues 513 in the cylinder 512.

[0039] A gap portion 516 is provided between the flange 502 and the outer wall of the cylinder 512. Each first ventilation hole 505 is aligned with each gap portion 516.

[0040] When the pipetting device 5 sucks liquid, the first air passage 507 is connected to negative pressure alone, and the liquid enters the pipette 501. Since the pipette 501 is relatively long, the liquid level will not reach the cylinder 512 far away.

[0041] When the pipetting device 5 discharges liquid, the first air passage 507 is connected to positive pressure alone.

[0042] When removing the pipette 501, positive pressure is applied to both the first air passage 507 and the second air passage 509 simultaneously. Gas enters the open end of the cylinder 512 through the central first ventilation hole 505. Since the filling block 517 reduces the gap between the telescopic tongues 513, the air pressure inside the inner cavity of the cylinder 512 is lower than that at the open end in a short time. Under the combined action of the air pressure difference and the gas impact force, the cone top block 514 moves downward to compress the spring 515. The cone top block 514 and the telescopic tongues 513 can be made of magnets. When the cone top block 514 moves downward, it can drive the telescopic tongues 513 to contract, releasing the flange 502. At the same time, since the liquid suction port at the lower end of the pipette 501 is relatively small, the positive pressure has a downward impact force on the pipette 501. At this time, the telescopic tongues 513 are in a released state. Under the combined action of gravity and the gas impact force, the pipette 501 drops. Under the action of the spring 515, the cone top block 514 resets. The telescopic tongues 513 are provided with limiting protrusions to limit the lateral displacement of the telescopic tongues 513, prevent the telescopic tongues 513 from being pushed out, and also prevent the pipette 501 from being squeezed and deformed.

[0043] When loading the pipette 501, the multi-axis moving mechanism 4 drives the base block 510 to move downward so that the sealing block 504 abuts against the open end of the pipette 501. At the same time, the second air passage 509 is separately connected to positive pressure to release the telescopic tongues 513. Subsequently, when the sealing block 504 moves down in place, the positive pressure is closed, and the telescopic tongues 513 are clamped on the flange 502.

[0044] In a preferred embodiment, a central fixing plate 204 is further provided. The first rotating motor 201 is installed on the central fixing plate 204. An intermediate connecting ring 206 is provided between the central fixing plate 204 and the first turntable 2. The intermediate connecting ring 206 is lower than the central fixing plate 204 and the first turntable 2. A plurality of first connecting columns 203 are provided along the circumferential direction at the lower end of the first turntable 2. A plurality of second connecting columns 205 are provided along the circumferential direction at the lower end of the central fixing plate 204. The lower ends of the first connecting columns 203 and the second connecting columns 205 are connected to the intermediate connecting ring 206.

[0045] The first turntable 2, the intermediate connecting ring 206, and the central fixing plate 204 form a concave disk structure to avoid the position of the second turntable 3.

[0046] The above embodiments are only the preferred technical solutions of the present invention and should not be regarded as limitations on the present invention. The protection scope of the present invention should be the technical solutions recorded in the claims, including the equivalent replacement solutions of the technical features in the technical solutions recorded in the claims. That is, the equivalent replacement improvements within this scope are also within the protection scope of the present invention.

Claims

1. A dual-turntable liquid transfer and centrifugation integrated device for film preparation, characterized in that: The invention comprises a bottom frame (1), wherein a first rotating disk (2) and a second rotating disk (3) are coaxially arranged and rotatable, the first rotating disk (2) is provided with a plurality of sampling bottle placement holes (202) along the circumference, the sampling bottle placement holes (202) are used to place sampling bottles, the second rotating disk (3) is provided with a plurality of film making bin placement grooves (302) along the circumference, the film making bin placement grooves (302) are used to place film making bins, and the bottom frame (1) is also provided with a multi-axis moving mechanism (4), the multi-axis moving mechanism (4) is provided with a liquid transfer device (5), and the liquid transfer device (5) is used to transfer liquid between the sampling bottle and the film making bin.

2. The dual-turntable liquid transfer and centrifugation integrated device for slice preparation according to claim 1, characterized in that: The bottom frame (1) comprises a bottom plate (101) and an upper plate (102); a first rotating motor (201) is provided on the bottom plate (101); the first rotating motor (201) drives the first rotating disk (2) to rotate; and a second rotating motor (301) is provided on the upper plate (102); the second rotating motor (301) drives the second rotating disk (3) to rotate.

3. The dual-turntable liquid transfer and centrifugation integrated device for slice preparation according to claim 2, characterized in that: A first through hole (103) and a second through hole (104) are provided on the upper plate (102); the pipetting device (5) comprises a pipette (501); the inner diameters of the first through hole (103) and the second through hole (104) are larger than the outer diameter of the pipette (501); the first through hole (103) is aligned with the film making chamber below; the second through hole (104) is aligned with the sampling bottle below; the pipette (501) is inserted into the film making chamber or the sampling bottle from below the first through hole (103) or the second through hole (104).

4. The dual-turntable liquid transfer and centrifugation integrated device for slice preparation according to claim 3, characterized in that: The multi-axis moving mechanism (4) comprises a first linear module (401), the first linear module (401) is connected to an upper plate (102), a second linear module (402) is provided on the first linear module (401), a third linear module (403) is provided on the second linear module (402), the displacement directions of the sliding plates of the first linear module (401), the second linear module (402) and the third linear module (403) are perpendicular to each other, a pipetting device (5) is connected to the sliding plate of the third linear module (403), a pipette storage bin (6) is further provided on the upper plate (102), the pipette storage bin (6) comprises a plurality of pipette placement holes (601) arranged in rows and columns, and the pipette placement holes (601) are used to place pipettes (501).

5. The dual-turntable liquid transfer and centrifugation integrated device for slice preparation according to claim 3, characterized in that: The liquid transfer device (5) comprises a base block (510), a tube holder (511) is provided at the lower end of the base block (510), the tube holder (511) comprises a column (512), the outer wall diameter of the column (512) is smaller than the inner diameter of the pipette (501), one end of the inner cavity of the column (512) is closed, an end of the column (512) close to the tube holder (511) is provided with an opening, a plurality of telescopic tongues (513) are provided along the circumferential direction of the open end of the column (512), a wedge-shaped surface is provided on the inner side of the telescopic tongue (513), a cone top block (514) is provided in the column (512), and the cone top block (514) is provided. 4) A spring (515) is provided at one end, the spring (515) abuts against the closed end of the column (512), the wedge-shaped surface of each telescopic tongue (513) abuts against the conical surface of the conical top block (514), an annular arc groove is provided on the outer wall of the telescopic tongue (513), a flange (502) is provided on the inner wall of the upper end of the pipette (501), the annular arc groove is clamped on the flange (502), a filling block (517) is provided between the telescopic tongues (513) in the column (512), a second air channel (509) is provided in the base block (510), and one end of the second air channel (509) is connected to the open end of the column (512).

6. The dual-turntable liquid transfer and centrifugation integrated device for slice preparation according to claim 5, characterized in that: A sealing block (504) is provided at the lower end of the base block (510), the sealing block (504) blocks the upper end of the pipette (501), a second vent hole (508) is provided in the center of the sealing block (504), the second vent hole (508) is communicated with the second air channel (509), the sealing block (504) is further provided with a plurality of first vent holes (505) arranged along the axial direction, the base block (510) is provided with a vent ring groove (506), the first vent holes (505) are communicated through the vent ring groove (506), and a first air channel (507) is further provided in the base block (510), one end of the first air channel (507) is communicated with the vent ring groove (506).

7. The dual-turntable liquid transfer and centrifugation integrated device for slice preparation according to claim 2, characterized in that: A central fixing plate (204) is also provided, the first rotating motor (201) is mounted on the central fixing plate (204), an intermediate connecting ring (206) is provided between the central fixing plate (204) and the first rotating disk (2), the intermediate connecting ring (206) is lower than the central fixing plate (204) and the first rotating disk (2), a plurality of first connecting columns (203) are provided along the circumferential direction at the lower end of the first rotating disk (2), a plurality of second connecting columns (205) are provided along the circumferential direction at the lower end of the central fixing plate (204), and the lower ends of the first connecting columns (203) and the second connecting columns (205) are connected to the intermediate connecting ring (206).

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