Polygonatum sibiricum seedling tissue culture device

Abstract

The utility model discloses a sealwort seedling tissue culture device and belongs to the technical field of seedling tissue culture. A sealwort seedling tissue culture device comprises a bottle body and further comprises a bottle cap detachably connected to the bottle body, an air hole communicated with the bottle body is formed in the bottle cap, a conversion plate is rotationally connected to the bottle cap, and the air hole can be blocked by the conversion plate; the connecting cylinder is fixedly connected to the conversion plate, the conversion plate is attached to the outer wall of the bottle cap, and a communicating hole is formed in the sleeve; when the conversion plate rotates and the through hole coincides with the air hole, the communicating hole is communicated with the cavity of the bottle body; the air holes can be blocked by rotating the conversion plate, at the moment, the sleeve can be detached from the connecting cylinder, then the tissue culture film is replaced, then the interior of the bottle body is blocked by the conversion plate, and the phenomenon that the tissue culture film is in contact with outside air when being replaced is prevented; part of dust in the air is prevented from entering the bottle body, and the sterile state of the interior of the bottle body is guaranteed.

Description

Technical Field

[0001] The utility model relates to the technical field of seedling tissue culture, in particular to a tissue culture device for polygonatum sibiricum seedlings. Background Art

[0002] Seedling tissue culture generally refers to tissue culture, also known as in vitro culture, which means separating the required tissues, organs or cells, protoplasts, etc. from the plant body, and through aseptic operation, culturing them under artificial control conditions to obtain regenerated complete plants or produce other products with economic value.

[0003] After retrieval, a patent with the Chinese patent publication number CN220756017U discloses a tissue culture device for polygonatum kingianum seedlings, which relates to the technical field of polygonatum kingianum seedlings. The tissue culture device for polygonatum kingianum seedlings includes a tissue culture bottle, a first bottle cap is arranged at the top of the tissue culture bottle, a first pipe penetrates and is fixedly connected to the top of the first bottle cap, a rotating shaft is rotatably connected to the top of the first pipe, and a baffle is fixedly connected to the outer surface of the rotating shaft; a clamping and fixing device is arranged inside the first pipe, and the clamping and fixing device includes a plug.

[0004] However, in the above patent, when the filter membrane of the tissue culture bottle needs to be replaced, the inside of the tissue culture bottle may come into contact with the outside air, resulting in some dust entering the inside of the tissue culture bottle and affecting the sterile state inside the tissue culture bottle. Summary of the Utility Model

[0005] The purpose of the utility model is to solve the problem that when the filter membrane needs to be replaced in the prior art, the inside of the tissue culture bottle may come into contact with the outside air, resulting in some dust entering the inside of the tissue culture bottle and affecting the sterile state inside the tissue culture bottle, and to propose a tissue culture device for polygonatum sibiricum seedlings.

[0006] In order to achieve the above purpose, the utility model adopts the following technical scheme:

[0007] A tissue culture device for polygonatum sibiricum seedlings includes a bottle body, and also includes: a bottle cap detachably connected to the bottle body, wherein, an air vent communicating with the bottle body is opened on the bottle cap, a conversion plate is rotatably connected to the bottle cap, and the conversion plate can block the air vent; a connecting cylinder fixedly connected to the conversion plate, and the conversion plate is attached to the outer wall of the bottle cap, a through hole is opened on the connecting cylinder, a sleeve is detachably connected to the connecting cylinder, and a communication hole is opened on the sleeve; when the conversion plate rotates and the through hole coincides with the air vent, the communication hole is communicated with the cavity of the bottle body.

[0008] In order to facilitate the connection of the conversion plate, preferably, a connecting column is arranged on the bottle cap, a connecting hole is opened on the conversion plate, and the connecting hole is attached to the outside of the connecting column.

[0009] In order to improve the sealing effect of the conversion plate, further, a groove is provided on the bottle cap, the connecting column is fixedly connected in the groove, and the conversion plate is fitted with the groove.

[0010] In order to facilitate the limiting and fixing of the limiting plate, preferably, a limiting plate is fixedly connected in the groove, and the conversion plate is matched with the limiting plate.

[0011] In order to facilitate the convenient replacement or installation of the tissue culture membrane, preferably, a clamping groove is provided on the connecting cylinder, a connecting groove is provided at the bottom of the sleeve, the connecting cylinder is clamped in the connecting groove, a clamping block is fixedly connected in the connecting groove, and the clamping block is detachably connected in the clamping groove.

[0012] In order to facilitate the detachable connection of the bottle cap, preferably, a threaded groove is provided on the outer wall of the bottle body, and the bottle cap is threadedly connected to the threaded groove.

[0013] Compared with the prior art, the utility model provides a tissue culture device for polygonatum sibiricum seedlings, which has the following beneficial effects:

[0014] 1. For this tissue culture device for polygonatum sibiricum seedlings, by rotating the conversion plate, the ventilation holes can be blocked. At this time, the sleeve can be detached from the connecting cylinder, and then the tissue culture membrane can be replaced. Furthermore, the conversion plate seals the inside of the bottle body, preventing contact with the outside air when replacing the tissue culture membrane, preventing some dust in the air from entering the inside of the bottle body, and ensuring the sterile state inside the bottle body.

[0015] 2. For this tissue culture device for polygonatum sibiricum seedlings, by placing the tissue culture membrane on the connecting cylinder and then sleeving the sleeve on the connecting cylinder, at this time, the clamping block will press the tissue culture membrane and push it into the clamping groove. At this time, the connecting groove fits with the outside of the connecting cylinder, and thus the tissue culture membrane can be conveniently installed.

[0016] In the device, the parts not involved are the same as or can be implemented by the prior art. In the utility model, by rotating the conversion plate, the ventilation holes can be blocked. At this time, the sleeve can be detached from the connecting cylinder, and then the tissue culture membrane can be replaced. Furthermore, the conversion plate seals the inside of the bottle body, preventing contact with the outside air when replacing the tissue culture membrane, preventing some dust in the air from entering the inside of the bottle body, and ensuring the sterile state inside the bottle body. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a three-dimensional structural schematic diagram of a tissue culture device for polygonatum sibiricum seedlings proposed by the utility model;

[0018] Figure 2 It is a structural schematic diagram of the bottle body of a tissue culture device for polygonatum sibiricum seedlings proposed by the utility model;

[0019] Figure 3 Schematic diagram of the structure of a partial tissue culture device for Polygonatum sibiricum seedlings proposed by the present utility model Figure 1 ;

[0020] Figure 4 Schematic diagram of the structure of a partial tissue culture device for Polygonatum sibiricum seedlings proposed by the present utility model Figure 2 ;

[0021] Figure 5 Schematic diagram of the structure of a partial tissue culture device for Polygonatum sibiricum seedlings proposed by the present utility model Figure 3 。

[0022] In the figure: 1, bottle body; 2, thread groove; 3, bottle cap; 4, groove; 5, connecting column; 6, ventilation hole; 7, limiting plate; 8, conversion plate; 9, connecting hole; 10, through hole; 11, connecting cylinder; 12, card slot; 13, sleeve; 14, communication hole; 15, connecting groove; 16, clamping block. Specific implementation mode

[0023] Next, the technical solutions in the embodiments of the present utility model will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present utility model. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments.

[0024] In the description of the present utility model, it should be understood that the orientation or positional relationship indicated by the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present utility model.

[0025] Embodiment:

[0026] Referring to Figures 1 - 5 , a tissue culture device for Polygonatum sibiricum seedlings includes a bottle body 1, and further includes: a bottle cap 3 detachably connected to the bottle body 1, wherein a ventilation hole 6 communicating with the bottle body 1 is provided on the bottle cap 3, a conversion plate 8 is rotatably connected to the bottle cap 3, and the conversion plate 8 can block the ventilation hole 6; a connecting cylinder 11 fixedly connected to the conversion plate 8, wherein the conversion plate 8 is attached to the outer wall of the bottle cap 3, a through hole 10 is provided on the connecting cylinder 11, a sleeve 13 is detachably connected to the connecting cylinder 11, and a communication hole 14 is provided on the sleeve 13; when the conversion plate 8 rotates and the through hole 10 coincides with the ventilation hole 6, the communication hole 14 is communicated with the cavity of the bottle body 1.

[0027] The Polygonatum sibiricum seedlings can be placed inside the bottle body 1. A threaded groove 2 is provided on the outer wall of the bottle body 1, and the bottle cap 3 is threadedly connected to the threaded groove 2. Subsequently, the bottle cap 3 is detachably connected to the threaded groove 2 of the bottle body 1, and the ventilation holes 6 on the bottle cap 3 can communicate with the cavity inside the bottle body 1. By rotating the conversion plate 8 on the bottle cap 3, the conversion plate 8 can block the ventilation holes 6. When it is necessary to seal the cavity inside the bottle cap 3, the conversion plate 8 can be rotated while being attached to the bottle cap 3 to misalign the connecting cylinder 11 on the bottle cap 3 with the ventilation holes 6, thereby using the conversion plate 8 to close the passage of the ventilation holes 6.

[0028] In addition, when it is necessary to connect the bottle body 1 to the external air, the conversion plate 8 can be rotated on the bottle cap 3 to align the connecting cylinder 11 with the ventilation holes 6. At this time, the cavity inside the bottle body 1 communicates with the external air. Meanwhile, a tissue culture membrane is provided on the connecting cylinder 11. This ventilation membrane can be a Viers tissue culture ventilation membrane, which is welded using a Viers polymer film. This material has the characteristics of being breathable but not permeable to water and bacteria, and can also make the bottle body 1 ventilate and isolate bacteria in a sealed state, providing a good environment for the propagation of tissue-cultured Polygonatum sibiricum. At the same time, the outside of the tissue culture membrane on the connecting cylinder 11 can be pressed and limited by the sleeve 13, so that the tissue culture membrane is arranged between the through hole 10 and the communication hole 14 for subsequent tissue culture work.

[0029] When it is necessary to conveniently replace the tissue culture membrane, the connecting hole 9 on the conversion plate 8 can be rotated to one side of the ventilation hole 6 to misalign the connecting hole 9 with the ventilation hole 6. During this process, the conversion plate 8 will block the ventilation hole 6. At this time, the sleeve 13 can be removed from the connecting cylinder 11, and then the tissue culture membrane can be replaced. The conversion plate 8 can block the inside of the bottle body 1 to prevent contact with the external air during the replacement of the tissue culture membrane and prevent some dust in the air from entering the inside of the bottle body 1, ensuring the aseptic state inside the bottle body 1.

[0030] Furthermore, in order to facilitate the convenient connection of the conversion plate 8 and improve the sealing performance of the conversion plate 8 during rotation, a connecting column 5 is provided on the bottle cap 3. A connecting hole 9 is provided on the conversion plate 8, and the connecting hole 9 fits externally with the connecting column 5. A groove 4 is provided on the bottle cap 3, and the connecting column 5 is fixedly connected in the groove 4, and the conversion plate 8 fits with the groove 4.

[0031] The conversion plate 8 can be connected and installed through the connecting column 5, and the conversion plate 8 is arranged in the groove 4, which can improve the sealing effect of the conversion plate 8 during rotation and can block or unseal the ventilation holes 6 at the same time.

[0032] Refer to Figure 1 and Figure 3As shown in the figure, a limiting plate 7 is fixedly connected in the groove 4, and the conversion plate 8 can cooperate with the limiting plate 7.

[0033] When the bottle body 1 needs to be in contact with the outside air through the tissue culture membrane, the right side wall of the conversion plate 8 can be abutted against the limiting plate 7. At this time, the through hole 10 coincides with the air permeable hole 6, thereby realizing the air permeable effect.

[0034] It should be explained that a clamping groove 12 is formed in the connecting cylinder 11, a connecting groove 15 is formed at the bottom of the sleeve 13, the connecting cylinder 11 is clamped in the connecting groove 15, a clamping block 16 is fixedly connected in the connecting groove 15, and the clamping block 16 is detachably connected in the clamping groove 12.

[0035] By placing the tissue culture membrane on the connecting cylinder 11 and then sleeving the sleeve 13 on the connecting cylinder 11, at this time, the clamping block 16 will press the tissue culture membrane and push it into the clamping groove 12. At this time, the connecting groove 15 fits with the outside of the connecting cylinder 11, thereby facilitating the installation of the tissue culture membrane.

[0036] The above is only a preferred specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution of the present invention and its inventive concept, makes equivalent substitutions or changes, and should be covered by the protection scope of the present invention.

Claims

1. A device for tissue culture of polygonatum sibiricum seedlings, comprising a bottle body (1), characterized in that: Also includes: a bottle cap (3) detachably connected to the bottle body (1), The bottle cap (3) is provided with a vent hole (6) communicating with the bottle body (1), and a conversion plate (8) is rotatably connected to the bottle cap (3), and the conversion plate (8) can block the vent hole (6); a connecting tube (11) fixedly connected to the conversion plate (8), The conversion plate (8) is fitted with the outer wall of the bottle cap (3), the connecting tube (11) is provided with a through hole (10), the connecting tube (11) is detachably connected with a sleeve (13), and the sleeve (13) is provided with a communicating hole (14); When the conversion plate (8) rotates and the through hole (10) coincides with the air-permeable hole (6), the communication hole (14) is connected to the cavity of the bottle body (1).

2. A device for tissue culture of polygonatum seedlings according to claim 1, characterized in that: The bottle cap (3) is provided with a connecting column (5), and the conversion plate (8) is provided with a connecting hole (9), and the connecting hole (9) is fitted with the outside of the connecting column (5).

3. A device for tissue culture of polygonatum seedlings according to claim 2, characterized in that: The bottle cap (3) is provided with a groove (4), the connecting column (5) is fixedly connected in the groove (4), and the conversion plate (8) is in close contact with the groove (4).

4. A device for tissue culture of polygonatum seedlings according to claim 3, characterized in that: A limit plate (7) is fixedly connected in the groove (4), and the conversion plate (8) cooperates with the limit plate (7).

5. The device for tissue culture of polygonatum sibiricum seedlings according to claim 1, characterized in that: The connecting tube (11) is provided with a clamping groove (12), the bottom of the sleeve (13) is provided with a connecting groove (15), the connecting tube (11) is clamped in the connecting groove (15), a connecting clamping block (16) is fixed in the connecting groove (15), and the clamping block (16) is detachably connected in the clamping groove (12).

6. A device for tissue culture of polygonatum seedlings according to claim 1, characterized in that: A thread groove (2) is provided on the outer wall of the bottle body (1), and the bottle cap (3) is threadably connected to the thread groove (2).

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