Affinity purification centrifugal column

Abstract

The affinity purification centrifugal column comprises a sample inner tube and a filtrate collecting tube arranged outside the sample inner tube in a sleeving manner, an affinity filler filling section is arranged at the lower part of the sample inner tube, an upper-layer filtering and fixing device is arranged on the upper side of the affinity filler filling section, and a lower-layer filtering and fixing device is arranged on the lower side of the affinity filler filling section; according to the affinity purification centrifugal column, different samples can be purified according to different fillers, the operation of the centrifugal column is simple, a user only needs to carry out simple coarse treatment on the samples to obtain centrifugal purification conditions, the purification can be completed without being matched with an ultra-speed centrifuge and a protein purification instrument, and the cost is low. And the experiment time and cost are greatly reduced.

Description

Technical Field

[0001] The utility model relates to the field of centrifuge columns, in particular to an affinity purification centrifuge column. Background Art

[0002] In R & D work, centrifuge columns are often used to perform affinity purification on samples. Most of the existing centrifuge columns add samples and reagents into the centrifuge columns and then centrifuge them to obtain purified samples. However, this type of centrifuge column has complex operations, low purification rate of the extracted samples, low efficiency of the existing centrifuge columns when performing large-scale sample purification, and the existing centrifuge columns are not convenient to carry, and also need to be used in conjunction with an ultracentrifuge and a protein purifier, making it inconvenient to use. Therefore, this application provides an affinity purification centrifuge column with high purification efficiency and wide applicability to meet market demands. Summary of the Utility Model

[0003] The technical problem to be solved by the utility model is: to solve the problem of low virus purification efficiency in the prior art, the utility model provides an affinity purification centrifuge column.

[0004] The technical solution adopted by the utility model to solve its technical problems is: an affinity purification centrifuge column, including a sample inner tube and a filtrate collection tube sleeved outside the sample inner tube; an affinity filler loading section is provided at the lower part of the sample inner tube, and different fillers can be filled in the affinity filler section to perform affinity purification of adeno-associated virus; purification of virus-like particles; purification of recombinant antibodies, purification of recombinant proteins, and nucleic acid purification, etc.; an upper layer filtration and fixing device is provided above the affinity filler loading section, and a lower layer filtration and fixing device is provided below it. The upper layer filtration and fixing device and the lower layer filtration and fixing device are used in cooperation to fix the filler in the affinity filler section and effectively filter out impurities in the buffer solution and the sample.

[0005] Further, the upper layer filtration and fixing device includes an upper layer snap ring, an upper layer filter sheet, and an upper layer mesh plastic gasket arranged in sequence from top to bottom. The upper layer snap ring can be used to fix the upper layer filter sheet to prevent it from floating. The upper layer filter sheet is used to filter the buffer solution or the sample and can fix the filler in the affinity filler loading section at the same time. The upper layer mesh plastic gasket is used to support the upper layer filter sheet to prevent the upper layer filter sheet from collapsing.

[0006] Further, the lower layer filtration and fixing device includes a lower layer snap ring, a lower layer filter sheet, and a lower layer mesh plastic gasket arranged in sequence from top to bottom. The lower layer snap ring is used to fix the lower layer filter sheet to prevent it from floating. The lower layer filter sheet is used to filter the buffer solution or the sample and fix the filler in the affinity filler section at the same time. The lower layer mesh plastic gasket is used to support the lower layer filter sheet to prevent the lower layer filter sheet from collapsing.

[0007] Further, the pore diameter of the upper filter is 0.5 - 2 microns. Preferably, the pore diameter of the upper filter is 0.5 microns. By setting the pore diameter of the upper filter to 0.5 microns, impurities in the buffer or sample can be filtered while effectively preventing the dispersion of the packing material.

[0008] Further, the pore diameter of the lower filter is 0.5 - 2 microns. Preferably, the pore diameter of the lower filter is 0.5 microns. Setting the pore diameter of the lower filter to 0.5 microns can filter out impurities in the buffer or sample well and also prevent the dispersion of the packing material.

[0009] Further, a liquid outlet is provided at the bottom of the sample inner tube. A clamping portion that is clamped to the filtrate collection tube is provided at the upper part of the sample inner tube, and the outer diameter of the clamping portion is the same as the inner diameter of the filtrate collection tube.

[0010] Further, a leak - proof cap for sealing the sample inner tube is connected to the top of the sample inner tube. The leak - proof cap includes a connecting portion for connecting to the sample inner tube, an opening - closing portion for facilitating the opening or closing of the cap body, and a sealing portion for plugging the sample inner tube to prevent the sample from flowing out during centrifugation; the sealing portion is a columnar structure, and the outer diameter of the sealing portion matches the inner diameter of the upper part of the sample inner tube.

[0011] Further, a threaded port is provided on the outer circumference of the top of the filtrate collection tube, and the threaded port is connected to a threaded cap.

[0012] Advantages of the present utility model:

[0013] The affinity purification centrifuge column of the present utility model can purify different samples according to different packing materials, and the operation of the centrifuge column is simple. The user only needs to perform simple rough treatment on the sample to meet the conditions for centrifugation purification, and it can complete purification without the need to cooperate with an ultra - speed centrifuge and a protein purification instrument, greatly reducing the experimental time and cost. Description of the drawings

[0014] The present utility model will be further described below in conjunction with the drawings and embodiments.

[0015] Figure 1 It is a schematic diagram of the affinity purification centrifuge column according to Embodiment 1 of the present utility model;

[0016] Figure 2 It is a schematic diagram of the sample inner tube according to Embodiment 1 of the present utility model;

[0017] Figure 3 It is a schematic diagram of the affinity purification centrifuge column according to Embodiment 2 of the present utility model;

[0018] Figure 4Schematic diagram of the filtrate collection tube described in Embodiment 2 of the present utility model;

[0019] In the figure, 1 is the sample inner tube, 2 is the filtrate collection tube, 3 is the affinity packing filling section, 4 is the upper layer filtering and fixing device, 41 is the upper layer snap ring, 42 is the upper layer filter, 43 is the upper layer mesh plastic gasket, 5 is the lower layer filtering and fixing device, 51 is the lower layer snap ring, 52 is the lower layer filter, 53 is the lower layer mesh plastic gasket, 6 is the liquid outlet, 7 is the clamping part, 8 is the leak-proof cover, 81 is the connecting part, 82 is the opening and closing part, 83 is the sealing part, 9 is the threaded port, and 10 is the threaded cover. Detailed implementation mode

[0020] The embodiments of the present utility model will be described in detail below. The examples of the embodiments are shown in the drawings, in which the same or similar reference numerals denote the same or similar elements or elements with the same or similar functions throughout. The embodiments described below with reference to the drawings are exemplary and are only used to explain the present utility model and should not be construed as a limitation of the present utility model.

[0021] In the description of the present utility model, it should be understood that the orientation or positional relationships indicated by the terms "upper", "lower", "horizontal", "top", "inner", etc. are based on the orientation or positional relationships shown in the drawings, and are only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present utility model.

[0022] In the description of the present utility model, it should be noted that unless otherwise clearly specified and defined, the term "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium. For those of ordinary skill in the art, the specific meanings of the above terms in the present utility model can be understood according to specific circumstances.

[0023] Embodiment 1

[0024] As Figure 1-2 shown, an affinity purification centrifuge column includes a sample inner tube 1 and a filtrate collection tube 2 sleeved outside the sample inner tube 1; an affinity packing filling section 3 is provided at the lower part of the sample inner tube 1, an upper layer filtering and fixing device 4 is provided on the upper side of the affinity packing filling section 3, and a lower layer filtering and fixing device 5 is provided on the lower side.

[0025] The upper - layer filtering and fixing device 4 includes an upper - layer clamping ring 41, an upper - layer filter sheet 42, and an upper - layer mesh plastic gasket 43 arranged in sequence from top to bottom; the lower - layer filtering and fixing device 5 includes a lower - layer clamping ring 51, a lower - layer filter sheet 52, and a lower - layer mesh plastic gasket 53 arranged in sequence from top to bottom. The pore diameter of the filter holes of the upper - layer filter sheet 42 is 0.5 microns; the pore diameter of the filter holes of the lower - layer filter sheet 52 is 0.5 microns.

[0026] A liquid outlet 6 is provided at the bottom of the sample inner tube 1, and a clamping portion 7 for clamping with the filtrate collection tube 2 is provided at the upper part of the sample inner tube 1. The outer diameter of the clamping portion 7 is the same as the inner diameter of the filtrate collection tube 2.

[0027] A leak - proof cap 8 for facilitating the sealing of the sample inner tube 1 is connected to the top of the sample inner tube 1. The leak - proof cap 8 includes a connecting portion 81 for connecting the sample inner tube 1, an opening - closing portion 82 for facilitating the opening or closing of the cap body, and a sealing portion 83 for plugging the sample inner tube 1 to prevent the sample from flowing out during centrifugation; the sealing portion 83 is a columnar structure, and the outer diameter of the sealing portion 83 matches the inner diameter of the upper part of the sample inner tube 1.

[0028] During use, first plug the lower - layer gasket, the lower - layer filter sheet 52, and the lower - layer clamping ring 51 at the lower half of the sample inner tube 1, add 50 μl of affinity purification filler, and after the filler settles under the action of gravity, then load the upper - layer gasket, the upper - layer filter sheet 42, and the upper - layer clamping ring 41 at the upper half; then sleeved the filtrate collection tube 2 outside the sample inner tube 1, and after tightening the lid, the assembly of the centrifugal column is completed.

[0029] Example 2

[0030] On the basis of Example 1, this example also provides an affinity purification centrifugal column, whose structure is substantially the same as that of the affinity purification centrifugal column described in Example 1. The difference is that, as Figure 3-4 shown, a threaded port 9 is provided on the outer ring at the top of the filtrate collection tube 2, and the threaded port 9 is connected to a threaded cap 10.

[0031] Example 3

[0032] On the basis of Example 1, this example also provides an affinity purification centrifugal column, whose structure is substantially the same as that of the affinity purification centrifugal column described in Example 1. The difference is that the pore diameter of the filter holes of the upper - layer filter sheet 42 is 1 micron, and the pore diameter of the filter holes of the lower - layer filter sheet 52 is 1 micron.

[0033] In this specification, the schematic description of the terms does not necessarily refer to the same embodiment. Moreover, the specific features, structures, materials, or characteristics described can be combined in a suitable manner in any one or more embodiments.

[0034] Based on the above-mentioned ideal embodiments of the present utility model as inspiration, through the above description, relevant staff can completely make various changes and modifications without departing from the technical idea of this utility model. The technical scope of this utility model is not limited to the content in the specification, and its technical scope must be determined according to the scope of the claims.

Claims

1. An affinity purification spin column, characterized in that: The invention comprises a sample inner tube (1) and a filtrate collecting tube (2) sleeved on the outside of the sample inner tube (1); an affinity filler loading section (3) is provided at the lower part of the sample inner tube (1); an upper filter fixing device (4) is provided on the upper side of the affinity filler loading section (3), and a lower filter fixing device (5) is provided on the lower side.

2. The affinity purification spin column according to claim 1, characterized in that The upper filter fixing device (4) comprises an upper clamping ring (41), an upper filter disc (42) and an upper mesh plastic gasket (43) which are arranged in sequence from top to bottom.

3. The affinity purification spin column according to claim 1, characterized in that The lower layer filter fixing device (5) comprises a lower layer clamping ring (51), a lower layer filter disc (52) and a lower layer mesh plastic gasket (53) which are arranged in sequence from top to bottom.

4. The affinity purification spin column according to claim 2, characterized in that The filter pores of the upper filter (42) have a diameter of 0.5-2 microns.

5. The affinity purification spin column according to claim 3, characterized in that The filter pore size of the lower filter (52) is 0.5-2 microns.

6. The affinity purification spin column according to claim 1, characterized in that The bottom of the sample inner tube (1) is provided with a liquid outlet (6), and the upper part of the sample inner tube (1) is provided with a clamping portion (7) for clamping with the filtrate collection tube (2), and the outer diameter of the clamping portion (7) is the same as the inner diameter of the filtrate collection tube (2).

7. The affinity purification spin column according to claim 6, characterized in that The top of the sample inner tube (1) is connected to a leakproof cover (8) for sealing the sample inner tube (1), and the leakproof cover (8) comprises a connecting portion (81) connected to the sample inner tube (1), an opening and closing portion (82) for opening or closing the cover body, and a sealing portion (83) for plugging the sample inner tube (1) to prevent the sample from flowing out during centrifugation; the sealing portion (83) is a columnar structure, and the outer diameter of the sealing portion (83) matches the inner diameter of the upper portion of the sample inner tube (1).

8. The affinity purification spin column according to claim 6, characterized in that The top outer ring of the filtrate collecting tube (2) is provided with a threaded opening (9), and the threaded opening (9) is connected to a threaded cover (10).

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