Separating and washing system for PHA fermentation liquor

Abstract

The utility model discloses a separating and washing system for PHA fermentation liquor, which is characterized in that a fermentation tank is respectively connected with a pipeline of a separator system, and the separator system is respectively connected with a first separation transfer tank and a second separation transfer tank; the first separation transfer tank and the second separation transfer tank are connected with the first washing buffer tank and the second washing buffer tank through pipelines; the first washing buffer tank and the second washing buffer tank are connected with a separator system pipeline; and the first separation transfer tank and the second separation transfer tank are connected with an enzymolysis system pipeline. Through the separator system, the separation transfer tank and the washing buffer tank, circulating separation and washing of the PHA fermentation liquor can be realized, the treatment efficiency is improved, the impurity content is reduced, and the pressure of a subsequent treatment process is relieved.

Description

Technical Field

[0001] The utility model belongs to the technical field of PHA production, and particularly relates to a separation and washing system for PHA fermentation broth. Background Art

[0002] PHA (polyhydroxyalkanoates) is a storage particulate synthesized by microorganisms under the condition of other nutrient limitations and excess carbon sources. It is also a biodegradable biopolymer polyester compound, with the characteristics of ordinary thermoplastic plastics. Different from traditional petrochemical plastics, PHA has good biocompatibility, biodegradability, piezoelectricity and optical activity, making it have broad application prospects in the fields of industry, agriculture, medicine and health, food, etc. In the fields of degradable agricultural films, degradable plastic products and medicine, PHA has shown great potential as the best biodegradable material at present.

[0003] In the process of fermentative production of PHA, it is necessary to separate cells from the fermentation broth, and then purify PHA in the separated bacteria. The current separation method generally uses a high-speed centrifuge for centrifugal separation. However, a large amount of reagents need to be added for lysis separation, resulting in high costs and serious pollution, making it difficult to achieve industrial production.

[0004] Chinese Patent CN1070534C discloses a method for separating and purifying intracellular polyhydroxyalkanoates from bacterial cells, in which it is necessary to use a solid-liquid separation method to concentrate the bacterial cells treated with an alkaline solution containing a surfactant for subsequent treatment processes.

[0005] Chinese Patent CN1800235A discloses a method for extracting high-purity PHAs from wet bacteria. First, it is necessary to concentrate the fermentation broth to collect the bacteria, and then dissolve the bacteria, separate and remove residues, and concentrate and separate to obtain PHA.

[0006] It can be seen that separation and concentration processes are required in the existing PHA extraction technologies. However, traditional membrane separation and concentration equipment is prone to pollution, low efficiency, and poor stability; a simple centrifuge has poor separation effects on light particles, and the above-mentioned equipment can only complete the separation and concentration process, and cannot help remove impurities contained in the fermentation broth, resulting in a large amount of reagents and time being consumed in subsequent PHA treatment processes to complete cell lysis and PHA extraction and purification. Therefore, it is necessary to develop a device that can increase the concentration of useful substances in PHA fermentation broth and reduce the impurity content. Summary of the Invention

[0007] In view of the above technical problems, the utility model provides a separation and washing system for PHA fermentation broth. Through the separator system and the washing buffer tank, the PHA fermentation broth can be separated and washed multiple times, so as to increase the concentration of the active ingredient and reduce the influence of impurities on the orderly process.

[0008] To achieve the above object, the utility model provides a separation and washing system for PHA fermentation broth. The fermentation tank is connected to the separator system through pipelines. The separator system is respectively connected to the first separation transfer tank and the second separation transfer tank; the first separation transfer tank and the second separation transfer tank are connected to the first washing buffer tank and the second washing buffer tank through pipelines; the first washing buffer tank and the second washing buffer tank are connected to the separator system through pipelines; the first separation transfer tank and the second separation transfer tank are connected to the enzymatic hydrolysis system through pipelines.

[0009] Preferably, the separator system includes a first separator, a second separator, a third separator and a fourth separator.

[0010] Further preferably, the first separator is connected in series with the second separator; the third separator is connected in series with the fourth separator.

[0011] Even more preferably, a fifth control valve is provided on the series pipeline of the first separator and the second separator; a sixth control valve is provided on the series pipeline of the third separator and the fourth separator.

[0012] Further preferably, first control valves, second control valves, third control valves and fourth control valves are respectively provided on the pipelines connecting the fermentation tank to the first separator, the second separator, the third separator and the fourth separator.

[0013] Further preferably, light phase outlets are respectively provided on the first separator, the second separator, the third separator and the fourth separator, and are connected to the wastewater treatment system through pipelines.

[0014] Further preferably, water inlets are respectively provided on the first separator, the second separator, the third separator and the fourth separator, and the water inlets are connected to the clean water storage tank through pipelines.

[0015] Preferably, washing water inlets are respectively provided on the first washing buffer tank and the second washing buffer tank, and the washing water inlets are connected to the washing water storage tank through pipelines.

[0016] Preferably, a seventh control valve is provided on the pipeline connecting the first separation transfer tank and the second separation transfer tank to the enzymatic hydrolysis system.

[0017] Preferably, the first separation transfer tank and the second separation transfer tank are connected to the separator system through pipelines.

[0018] The beneficial effects of the utility model are as follows:

[0019] 1. A separator system composed of four separators can accommodate all the fermentation broth produced by the fermenter at one time, empty the fermentation broth at one time, enable the fermenter to quickly enter the next production process, and improve production efficiency; at the same time, the simultaneous operation of the four separators can significantly improve the separation efficiency.

[0020] 2. The separator is connected to the separation transfer tank and the washing buffer tank to collect and temporarily store the separated liquid, and then wash it. The washed solution is transported back to the separator through the pipeline for separation and concentration. Through the control of pipelines and valves, multiple cycles of washing and concentration can be achieved, reducing the content of impurities in the fermentation broth, increasing the concentration of effective substances, and reducing the pressure of subsequent treatment processes. Brief Description of the Drawings

[0021] Figure 1 It is a schematic diagram of the overall structure of the present invention. In the figure, 1 is the fermenter, 2 is the first separator, 3 is the second separator, 4 is the third separator, 5 is the fourth separator, 6 is the first separation transfer tank, 7 is the second separation transfer tank, 8 is the first washing buffer tank, 9 is the second washing buffer tank, 10 is the wastewater treatment system, 11 is the enzymolysis system, 12 is the first control valve, 13 is the second control valve, 14 is the third control valve, 15 is the fourth control valve, 16 is the fifth control valve, 17 is the sixth control valve, 18 is the seventh control valve, 19 is the washing water storage tank, and 20 is the clean water storage tank. Detailed Embodiments

[0022] The technical solutions of the present invention will be further explained below with reference to the drawings and specific embodiments. It should be noted that the following embodiments are only the preferred embodiments of the present invention and should not be construed as a limitation of the present invention. The protection scope of the present invention should be based on the content recorded in the claims. Modifications and substitutions made by those skilled in the art to the technical solutions of the present invention without creative efforts fall within the protection scope of the present invention.

[0023] Embodiment 1

[0024] As Figure 1As shown in the figure, a separation and washing system for PHA fermentation broth. The fermenter 1 is respectively connected to the separator system through pipelines. The separator system is respectively connected to the first separation transfer tank 6 and the second separation transfer tank 7 to preliminarily separate and concentrate the fermentation broth, and then store the concentrated fermentation broth in the separation transfer tank. The first separation transfer tank 6 and the second separation transfer tank 7 are connected to the first washing buffer tank 8 and the second washing buffer tank 9 through pipelines to perform a primary washing on the separated and concentrated fermentation broth. The first washing buffer tank 8 and the second washing buffer tank 9 are connected to the separator system through pipelines to separate and concentrate the washed liquid again. The first separation transfer tank 6 and the second separation transfer tank 7 are connected to the enzymolysis system 11 through pipelines to perform enzymolysis on the separated and concentrated liquid for subsequent processing.

[0025] Preferably, the separator system includes a first separator 2, a second separator 3, a third separator 4 and a fourth separator 5. The capacity of the four separators ≥ the volume of the fermenter 1, and all the fermentation broth in the fermenter 1 can be accommodated at one time, ensuring that the fermenter 1 is emptied at one time. The emptied fermenter 1 can be immediately put into the next round of production, improving production efficiency.

[0026] More preferably, the first separator 2 is connected in series with the second separator 3; the third separator 4 is connected in series with the fourth separator 5 to perform multiple separations on the liquid, improving the separation and concentration efficiency.

[0027] Even more preferably, a fifth control valve 16 is provided on the series pipeline of the first separator 2 and the second separator 3; a sixth control valve 17 is provided on the series pipeline of the third separator 4 and the fourth separator 5. When the liquid needs to be separated multiple times synchronously, the fifth control valve 16 and the sixth control valve 17 are opened to perform multiple separation and concentration, improving the efficiency.

[0028] More preferably, first control valves 12, second control valves 13, third control valves 14 and fourth control valves 15 are respectively provided on the pipelines connecting the fermenter 1 to the first separator 2, the second separator 3, the third separator 4 and the fourth separator 5 to control the amount of liquid entering the separator, avoiding overlimit, and at the same time, the liquid can be dispersed for separation treatment, improving the treatment efficiency.

[0029] More preferably, light phase outlets are respectively provided on the first separator 2, the second separator 3, the third separator 4 and the fourth separator 5, and are connected to the wastewater treatment system 10 through pipelines to discharge the separated light phase solution that does not contain or contains a small amount of bacteria for wastewater treatment.

[0030] More preferably, water inlets are respectively provided on the first separator 2, the second separator 3, the third separator 4 and the fourth separator 5. The water inlets are connected to the clean water storage tank 20 through pipelines to add clean water to the separator system, concentrating while separating and performing preliminary washing.

[0031] Preferably, the first washing buffer tank 8 and the second washing buffer tank 9 are respectively provided with washing water inlets, and the washing water inlets are connected to the washing water storage tank 19 through pipelines. During operation, clear water is continuously replenished into the washing buffer tanks for in-depth washing.

[0032] Preferably, a seventh control valve 18 is provided on the pipelines connecting the first separation transfer tank 6 and the second separation transfer tank 7 to the enzymatic hydrolysis system 11. After separation and washing are completed, the solution is temporarily stored in the first separation transfer tank 6 and the second separation transfer tank 7, and then the seventh control valve 18 is opened to discharge the solution after separation and washing into the enzymatic hydrolysis system for the next process treatment.

[0033] Preferably, the first separation transfer tank 6 and the second separation transfer tank 7 are connected to the separator system through pipelines.

[0034] Example 2

[0035] During operation, the fermentation broth is completely discharged from the fermentation tank 1 and enters the first separator 2, the second separator 3, the third separator 4, and the fourth separator 5 respectively for synchronous separation and concentration. The light phase separated is discharged to the wastewater treatment system to obtain a primary concentrated solution.

[0036] The primary concentrated solution is respectively discharged into the first separation transfer tank 6 and the second separation transfer tank 7 for storage, and then enters the first washing buffer tank 8 and the second washing buffer tank 9 respectively. Clear water is added into them by using the washing water storage tank 19 for the first in-depth washing to obtain a primary washing solution.

[0037] The primary washing solution is respectively transported to the first separator 2 and the second separator 3 through pipelines for concentration separation, and then the treated solution is stored in the first separation transfer tank 6, and then sent to the third separator 4 and the fifth separator 5. Clear water is added online to the third separator 4 and the fifth separator 5 by using the clear water storage tank 20 for washing and concentrating simultaneously for the second washing to obtain a secondary washing solution.

[0038] The secondary washing solution is stored in the second separation transfer tank 7, and then is sent into the enzymatic hydrolysis system 11 through the seventh control valve 18 for enzymatic hydrolysis treatment.

Claims

1. A separation and washing system for PHA fermentation broth, characterized in that: The fermentation tank (1) is respectively connected to the separator system pipeline, and the separator system is respectively connected to the first separation transfer tank (6) and the second separation transfer tank (7); the first separation transfer tank (6) and the second separation transfer tank (7) are respectively connected to the first washing buffer tank (8) and the second washing buffer tank (9) through pipelines; the first washing buffer tank (8) and the second washing buffer tank (9) are connected to the separator system pipeline; the first separation transfer tank (6) and the second separation transfer tank (7) are connected to the enzymolysis system (11) through pipelines.

2. A separation and washing system for PHA fermentation broth according to claim 1, characterized in that: The separator system comprises a first separator (2), a second separator (3), a third separator (4) and a fourth separator (5).

3. A separation and washing system for PHA fermentation broth according to claim 2, characterized in that: The first separator (2) is connected in series with the second separator (3); the third separator (4) is connected in series with the fourth separator (5).

4. A separation and washing system for PHA fermentation broth according to claim 3, characterized in that: A fifth control valve (16) is provided on the serial pipeline between the first separator (2) and the second separator (3); and a sixth control valve (17) is provided on the serial pipeline between the third separator (4) and the fourth separator (5).

5. A separation and washing system for PHA fermentation broth according to claim 2, characterized in that: A first control valve (12), a second control valve (13), a third control valve (14) and a fourth control valve (15) are respectively provided on pipelines connecting the fermentation tank (1) with the first separator (2), the second separator (3), the third separator (4) and the fourth separator (5).

6. A separation and washing system for PHA fermentation broth according to claim 2, characterized in that: The first separator (2), the second separator (3), the third separator (4) and the fourth separator (5) are respectively provided with a light phase outlet which is connected to a pipeline of a wastewater treatment system (10).

7. A separation and washing system for PHA fermentation broth according to claim 2, characterized in that: The first separator (2), the second separator (3), the third separator (4) and the fourth separator (5) are respectively provided with a water inlet, which is connected to a pipeline of a clean water storage tank (20).

8. The separation and washing system for PHA fermentation broth according to claim 1, characterized in that: The first washing buffer tank (8) and the second washing buffer tank (9) are respectively provided with washing water inlets, and the washing water inlets are connected to the washing water storage tank (19) by pipeline.

9. A separation and washing system for PHA fermentation broth according to claim 1, characterized in that: A seventh control valve (18) is provided on the pipelines connecting the first separation transfer tank (6) and the second separation transfer tank (7) with the enzymatic hydrolysis system (11).

10. A separation and washing system for PHA fermentation broth according to claim 1, characterized in that: The first separation transfer tank (6) and the second separation transfer tank (7) are connected to the separator system via pipelines.

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