Temperature-control flat plate pouring device
Through the design of the temperature-controlled inverted plate device, the problems of inverted plate temperature control and uneven inverted plates are solved, the effectiveness and safety of antibiotics are improved, and the risk of exposure to high temperatures by experimenters is reduced.
Patent Information
- Application Number
- CN202422329012.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-24
- Publication Date
- 2025-07-01
- Estimated Expiration
- 2034-09-24
AI Technical Summary
In the prior art, the temperature of the culture medium cannot be controlled, resulting in antibiotic inactivation and medium solidification, and the thickness is uneven during the inverting plate, which poses a risk of manual contact between experimenters.
A temperature-controlled inverted flat plate device is designed, including a box rack, oscillator, sterilization bottle, heating coil and thermometer. The temperature of the sterilization bottle is controlled through the thermometer, and combined with a fixed volume tube and oscillator to achieve accurate control of flow rate and volume to avoid solidification of the culture medium and antibiotic inactivation.
Accurate control of the medium temperature is achieved, antibiotic inactivation and medium solidification is avoided, the accuracy and safety of the inverted plates are improved, and the work intensity and danger of the experimenter are reduced.
Smart Images

Figure CN223047527U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of test instrument equipment, in particular to a temperature-controlled plate pouring device. Background Art
[0002] In laboratory-related activities, antibiotics are often added to LB solid medium to screen specific resistant colonies. "Pouring a plate" means pouring the liquid agar medium sterilized at high temperature into a culture dish and cooling it to solidify into a solid medium. In the traditional plate pouring process, since antibiotics will lose their activity at high temperatures and the LB medium will solidify at lower temperatures, it is necessary to pour the plate within a suitable temperature range. Experimenters often rely on hand touch to empirically judge the temperature. This process may not only result in misjudgment, but also make the experimenter's hands feel extremely uncomfortable at higher temperatures. In addition, the amount of medium poured and the shaking all need to be completed based on experience, and there are often situations where the thickness of the solid medium is uneven and the plane is inclined.
[0003] As disclosed in the patent application No. CN213943169U, "A Plate Pouring Device for Laboratory Use", it includes an inner container located in a cavity. A small steam sterilization device is provided inside the main body. A steam outlet connected to the small steam sterilization device through a steam pipe is provided in the cavity. The lower end of the inner container is connected to an outlet pipe, and a valve plate for blocking the outlet pipe is provided on the side of the main body.
[0004] Although the prior art avoids hand contact with the medium and reduces the labor intensity and danger of personnel, during the LB plate pouring operation, it is impossible to judge when to add antibiotics, and there may also be a situation where the medium solidifies after cooling and blocks the outlet, making it difficult to clean. At the same time, its automatic pouring can only control the switch, and the water pressure plus the steam pressure result in inconsistent water flow rates, easily causing overflow or being too thin. Summary of the Invention
[0005] The utility model mainly solves the deficiencies in the prior art that the temperature of the medium cannot be controlled and the thickness of the poured plate is uneven, and provides a temperature-controlled plate pouring device, which has the characteristics of simple structure, convenient adjustment operation and good operation stability. It solves the problems of antibiotic inactivation and medium solidification during the process. It avoids the danger of experimental personnel manually contacting the high-temperature medium.
[0006] The above technical problems of the utility model are mainly solved by the following technical solutions:
[0007] A temperature-controlled plate-pouring device, comprising a box frame. An oscillator is provided inside the box frame. A sterilization bottle extending out of the upper end of the box frame is provided above the box frame. A heating coil nested with the sterilization bottle is provided around the sterilization bottle. A constant-volume tube extending above the oscillator is provided at the lower end of the sterilization bottle. A support frame opening Ⅰ inserted with the sterilization bottle is provided between the box frame and the sterilization bottle. A bottle mouth is provided at the upper end of the sterilization bottle. A liquid outlet pipe inserted with the constant-volume tube is provided at the lower end of the sterilization bottle.
[0008] Preferably, a thermometer is provided on the side of the front end of the box frame and is in communication with the heating coil. A support frame opening Ⅱ is provided on the side of the support frame opening Ⅰ. A temperature-measuring probe electrically connected to the thermometer is provided on the support frame opening Ⅱ.
[0009] Preferably, the heating coil includes an insulating housing. A ceramic heating wall is provided between the insulating housing and the sterilization bottle. A heating coil plug in communication with the thermometer is provided on the insulating housing.
[0010] Preferably, the insulating housing is fastened by a locking member. The locking member consists of a pair of locking blocks and a screw pin.
[0011] Preferably, a thermometer display screen is provided above the thermometer. A thermometer plug is provided at the lower end of the thermometer. A heating coil socket electrically connected to the heating coil plug is provided below the thermometer. A temperature-setting button is provided between the heating coil socket and the thermometer display screen.
[0012] Preferably, an oscillation tray is provided at the upper end of the oscillator. An oscillator display screen is provided at the front end of the oscillator. A number of speed-adjusting buttons are provided on the side of the oscillator display screen.
[0013] Preferably, the constant-volume tube includes a constant-volume tube body. A rubber sleeve is provided between the upper end of the constant-volume tube body and the liquid outlet pipe. A two-way cock Ⅱ is provided at the lower part of the constant-volume tube body.
[0014] Preferably, a two-way cock Ⅰ is provided on the liquid outlet pipe.
[0015] Preferably, handles are provided on both sides of the upper part of the sterilization bottle. A number of support legs distributed in a ring shape are provided at the lower end of the sterilization bottle.
[0016] The utility model can achieve the following effects:
[0017] The utility model provides a temperature-controlled pour plate device, which has the characteristics of simple structure, convenient adjustment operation and good running stability compared with the prior art. Through an externally connected temperature control device, the temperature of the culture medium is maintained within a set range, avoiding the problems of antibiotic inactivation and medium solidification midway, and improving the effectiveness and utilization rate of the resistant medium. Through the sterilization bottle piston and the constant volume device, the control of the flow rate and the volume of the culture medium is completed, improving the accuracy of the work. By replacing manual shaking with an oscillator, the contact between the experimenter and the high-temperature culture medium is avoided, reducing the work intensity and danger of the experimenter. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] Figure 1 is a schematic structural diagram of the utility model.
[0019] Figure 2 is a schematic structural diagram of the temperature-controlled dropping liquid assembly of the utility model.
[0020] Figure 3 is a schematic structural diagram of the temperature-controlled dropping liquid assembly of the utility model.
[0021] Figure 4 is a schematic structural diagram of the temperature-controlled dropping liquid assembly of the utility model.
[0022] In the figure: box frame 1, support frame opening I 101, support frame opening II 102, sterilization bottle 2, bottle mouth 201, handle 202, liquid outlet pipe 203, two-way cock I 204, support leg 205, thermometer 3, thermometer display screen 301, temperature setting button 302, heating coil socket 303, thermometer plug 304, temperature measuring probe 305, oscillator 4, oscillation tray 401, oscillator display screen 402, speed regulation button 403, constant volume tube 5, rubber sleeve 501, constant volume tube body 502, two-way cock II 503, heating coil 6, locking part 601, insulating shell 602, heating coil plug 603, ceramic heating wall 604. DETAILED DESCRIPTION OF THE EMBODIMENTS
[0023] The technical solutions of the present utility model will be further specifically described below through examples and in conjunction with the drawings.
[0024] Example: As Figures 1-4As shown in the figure, a temperature-controlled pour plate device includes a box frame 1. Inside the box frame 1, there is an oscillator 4. At the upper end of the oscillator 4, there is an oscillation tray 401. At the front end of the oscillator 4, there is an oscillator display screen 402. There are 4 speed adjustment buttons 403 on the side of the oscillator display screen 402. There are also a power-on button and a power-off button beside the speed adjustment buttons 403. Above the box frame 1, there is a sterilization bottle 2 extending out of the upper end of the box frame 1. Between the box frame 1 and the sterilization bottle 2, there is a support frame opening I 101 inserted into the sterilization bottle 2. At the upper end of the sterilization bottle 2, there is a bottle mouth 201. At the lower end of the sterilization bottle 2, there is a liquid outlet pipe 203 inserted into a constant volume tube 5. On the liquid outlet pipe 203, there is a two-way cock I 204. On both sides of the upper part of the sterilization bottle 2, there are handles 202. At the lower end of the sterilization bottle 2, there are 3 support legs 205 distributed in a ring. Around the sterilization bottle 2, there is a heating coil 6 nested with the sterilization bottle 2. The heating coil 6 includes an insulating shell 602, and the insulating shell 602 is fastened by a locking part 601. The locking part 601 consists of a pair of locking blocks and a screw pin. Between the insulating shell 602 and the sterilization bottle 2, there is a ceramic heating wall 604. On the insulating shell 602, there is a heating coil plug 603 in communication with a thermometer 3. At the lower end of the sterilization bottle 2, there is a constant volume tube 5 extending above the oscillator 4. The constant volume tube 5 includes a constant volume tube body 502. Between the upper end of the constant volume tube body 502 and the liquid outlet pipe 203, there is a rubber sleeve 501. At the lower part of the constant volume tube body 502, there is a two-way cock II 503. On the side of the front end of the box frame 1, there is a thermometer 3 in communication with the heating coil 6. Beside the support frame opening I 101, there is a support frame opening II 102. On the support frame opening II 102, there is a temperature measuring probe 305 in electrical communication with the thermometer 3. At the upper part of the thermometer 3, there is a thermometer display screen 301. At the lower end of the thermometer 3, there is a thermometer plug 304. At the lower part of the thermometer 3, there is a heating coil socket 303 in electrical communication with the heating coil plug 603. Between the heating coil socket 303 and the thermometer display screen 301, there is a temperature setting button 302.
[0025] Working principle: When the two-way cock I 204 is closed, the sterilization bottle 2 is poured with agar medium through the bottle mouth 201 for high-pressure steam sterilization. The support legs 205 support the sterilization bottle 2 during sterilization. After sterilization, the sterilization bottle 2 is supported on the opening I 101 of the support frame through the handle 202, and the shape of the opening I 101 of the support frame allows the bottle body and the support legs 205 of the sterilization bottle 2 to pass through. The temperature measurement probe 305 of the thermometer 3 enters the bottle mouth 201 through the opening II 102 of the support frame to measure the temperature. The radius of the heating coil 6 is slightly smaller than the radius of the bottle body. After opening the locking member 601, it is sleeved on the bottle body of the sterilization bottle 2, and the two locking blocks on both sides are tightened by relying on the locking member 601 through the threaded pin, thereby increasing the pressure on the sterilization bottle 2. The ceramic heating wall 604 is heated, and the insulating shell 602 is used to avoid high-temperature scalding when contacting. The heating coil plug 603 is connected to the thermometer 3 through the heating coil socket 303, and the thermometer 3 sets the upper and lower limits of the temperature range through the temperature setting button 302. The set temperature value and the measured temperature value are both displayed on the thermometer display screen 301. When the measured temperature is lower than the lower limit of the set value, the heating coil socket 303 is powered on to control the heating of the heating coil 6. When the measured temperature is higher than the upper limit of the set value, the heating coil socket 303 is powered off to cool down. After reaching the appropriate temperature range, antibiotics are added from the bottle mouth 201 at the set temperature.
[0026] When pouring the flat plate, the constant volume tube 5 is connected to the end of the two-way cock I 204 through the rubber sleeve 501 to realize the assembly and disassembly of the constant volume tube 5. Open the two-way cock I 204, and the agar medium located in the set temperature value range and with antibiotics added is introduced into the constant volume tube 5 through the liquid outlet pipe 203 and the orifice of the two-way cock I 204. The constant volume tube body 502 is marked with scales. When the target scale is reached, close the two-way cock I 204 and open the two-way cock II 503 to drip a certain volume of agar culture on the culture dish placed on the oscillation tray 401. The oscillation speed is displayed on the oscillation instrument display screen 402. The operator adjusts the speed control button 403 to adjust the oscillation instrument 4 to a certain speed so that the culture medium dropped on the culture dish is evenly distributed around the culture dish.
[0027] In summary, the temperature-controlled flat plate pouring device has the characteristics of simple structure, convenient adjustment operation and good operation stability. Through the external temperature control device, the temperature of the culture medium is maintained within the set range, avoiding the problems of antibiotic inactivation and medium solidification midway, and improving the effectiveness and utilization rate of the resistant medium. Through the sterilization bottle piston and the constant volume device, the control of the flow rate and the volume of the culture medium is completed, improving the accuracy of the work. By replacing manual shaking with an oscillator, the contact between the experimenter and the high-temperature culture medium is avoided, reducing the work intensity and danger of the experimenter.
[0028] For those skilled in the art, it is obvious that the present utility model is not limited to the details of the above exemplary embodiments, and the present utility model can be implemented in other specific forms without departing from the basic characteristics of the utility model. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting. The scope of the present utility model is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be embraced within the present utility model. Any reference signs in the claims should not be construed as limiting the claims involved.
[0029] In summary, the above are only specific embodiments of the present utility model, but the structural features of the present utility model are not limited thereto. Any changes or modifications made by those skilled in the art within the field of the present utility model are covered by the patent scope of the present utility model.
Claims
1. A temperature-controlled flat plate inverting device, characterized in that: The invention comprises a box frame (1), wherein an oscillator (4) is arranged inside the box frame (1), a sterilization bottle (2) extending from the upper end of the box frame (1) is arranged on the upper part of the box frame (1), a heating coil (6) nested with the sterilization bottle (2) is arranged on the outer periphery of the sterilization bottle (2), and a constant volume tube (5) extending to the upper part of the oscillator (4) is arranged at the lower end of the sterilization bottle (2); a support frame opening I (101) plugged into the sterilization bottle (2) is arranged between the box frame (1) and the sterilization bottle (2), a bottle mouth (201) is arranged at the upper end of the sterilization bottle (2), and a liquid outlet tube (203) plugged into the constant volume tube (5) is arranged at the lower end of the sterilization bottle (2).
2. A temperature-controlled inverted plate device according to claim 1, characterized in that: The front end side of the box frame (1) is provided with a thermometer (3) which is in circuit communication with the heating coil (6), the side of the support frame opening I (101) is provided with a support frame opening II (102), and the support frame opening II (102) is provided with a temperature measuring probe (305) which is in circuit communication with the thermometer (3).
3. A temperature-controlled inverted plate device according to claim 2, characterized in that: The heating coil (6) comprises an insulating shell (602), a ceramic heating wall (604) is provided between the insulating shell (602) and the sterilization bottle (2), and a heating coil plug (603) which is in communication with the thermometer (3) is provided on the insulating shell (602).
4. A temperature-controlled inverted plate device according to claim 3, characterized in that: The insulating housing (602) is fastened by a locking member (601), and the locking member (601) is composed of a pair of locking blocks and a screw pin.
5. A temperature-controlled inverted plate device according to claim 3, characterized in that: The thermometer (3) is provided with a thermometer display screen (301) at the top, a thermometer plug (304) is provided at the bottom of the thermometer (3), a heating coil socket (303) which is in circuit communication with the heating coil plug (603) is provided at the bottom of the thermometer (3), and a temperature setting button (302) is provided between the heating coil socket (303) and the thermometer display screen (301).
6. A temperature-controlled inverted plate device according to claim 1, characterized in that: An oscillator tray (401) is provided at the upper end of the oscillator (4), an oscillator display screen (402) is provided at the front end of the oscillator (4), and a plurality of speed adjustment buttons (403) are provided on the side of the oscillator display screen (402).
7. The temperature-controlled inverted plate device according to claim 1, characterized in that: The constant volume tube (5) comprises a constant volume tube body (502), a rubber sleeve (501) is provided between the upper end of the constant volume tube body (502) and the liquid outlet pipe (203), and a two-way cock II (503) is provided at the lower part of the constant volume tube body (502).
8. The temperature-controlled inverted plate device according to claim 1, characterized in that: The liquid outlet pipe (203) is provided with a two-way cock I (204).
9. The temperature-controlled inverted plate device according to claim 1, characterized in that: The sterilization bottle (2) is provided with handles (202) on both sides of the upper portion, and the sterilization bottle (2) is provided with a plurality of supporting legs (205) distributed in a ring shape at the lower end.
Citation Information
Patent Citations
Flat plate reversing device for laboratory
CN213943169U